Can plantations develop understory biological and physical attributes of naturally regenerated forests?

With an increasing proportion of natural forests being replaced by plantations, there is a need to determine their potential to fulfill ecological purposes other than wood production. This study evaluated the extent to which deciduous and coniferous plantations develop understory attributes comparable to those of naturally regenerated stands. A functional group approach was used to synthesise species responses in terms of their ecological traits. Multivariate analyses of ecological traits revealed 16 emergent groups that shared common traits associated with a similar life history strategy. Responses of these groups, understory structure, and understory environmental conditions to plantation types and stand stages were analyzed and compared to naturally regenerated stands. Clear associations of trait responses to stand developmental stages and plantation types emerged. Light-demanding and wind-dispersed species groups were associated with early-successional stages, while woody groups, ferns and ant-dispersed spring-flowering herbs were associated with late-successional stages. Analyses also revealed an indicator group associated with old naturally regenerated forest. The understory functional groups and environmental conditions of deciduous plantations converged toward those of old naturally regenerated forests. However, understory structure in deciduous plantations remained poorly developed and richness of the indicator group was low compared to unplanted stands. Conifer plantations, currently the most common plantation type in the northern hardwood biome, showed a completely different pathway of understory development. Modifications to current plantation management practices are proposed to help recreate or maintain natural understory biological and physical attributes.     

Understory plant species and functional diversity in the degraded wet tropical forests of Kolombangara Island,Solomon Islands

Anthropogenic activities have resulted in extensive deforestation and forest degradation on many tropical oceanic islands. For instance, some islands in the Solomon archipelago have as little as 10% of primary forests remaining with few of these remnants protected from future land use change. We examine the plant species and functional diversity (excluding adult canopy trees) of 48 sites from four forest land use types (two types of primary forest, secondary forest and abandoned tree plantations) and two common human-maintained land use types (coconut plantations and grazed pastures) across three elevation bands on Kolombangara Island, Solomon Islands. In total, we surveyed 384 species from 86 families of which only 6.5% were non-native. Species richness was lowest in coconut plantations and grazed pastures and declined with increasing elevation across all land use types. Functional diversity was similar between primary and secondary forest (high richness, high evenness and unaltered dispersion) and lowest in coconut plantations and grazed pastures. Our results suggest that species and functional richness have had divergent responses to land use change in forest land uses indicative of a loss of functional redundancy. Despite structural and compositional similarities among primary forests and degraded forest land uses, full recovery of secondary and commercial plantations has not been achieved. We suggest that conservation of Kolombangara’s forest understory flora will require reserves across the island’s elevation gradient and may require active restoration in the future, particularly if degrading activities continue at the current rate.     

Urbanization and riparian forest woody communities: Diversity, composition, and structure within a metropolitan landscape

Understanding how urban land-use structure contributes to the abundance and diversity of riparian woody species can inform management and conservation efforts. Yet, previous studies have focused on broad-scale (e.g., urban to exurban) land-use types and have not examined more local-scale changes in land use (e.g., the variation within “urban”), which could be important in urban areas. In this paper we examine how local-scale characteristics or fine-scale urban heterogeneity affect(s) the diversity, composition, and structure of temperate woody riparian vegetation communities in the highly urbanized area of Cincinnati, Ohio, USA. We use an information-theoretic approach to compare vegetation models and canonical correspondence analyses to compare species responses to urban variables. We found that urban riparian areas can harbor a high diversity of native canopy and shrub species (38 and 41, respectively); however, native and exotic woody plant species responded differently to urbanization. Exotic canopy species increased with the level of urbanization while native canopy and understory species declined. Understory species diversity displayed a greater response to urbanization than did canopy diversity, suggesting temporal lags in canopy response to disturbances associated with present and recent land-use changes. Certain native and exotic woody species represent ecological indicators of different levels of urbanization. Native species characteristic of pre-European settlement conditions were restricted to the wide riparian forests with little urban encroachment. Several native early-successional species appear tolerant to urbanization. Two exotic species, the tree Ailanthus altissima and the shrub Lonicera maackii, were the most abundant and ubiquitous woody species and appear to exploit urban disturbances. These exotic species invasions have the potential to modify forest composition and ecological function of urban riparian systems. In addition, altered hydrology may be a contributing factor as canopy and understory stem density of high-moisture-requiring species decreased with an increase in impervious surface and grass cover and with proximity to roads and railroads. In the face of urbanization, maintaining wide riparian forests and limiting building, road and railroad development within these areas may help reduce the invasion of exotic species and benefit hydrological function in temperate riparian areas.     

Eucalypt plantations as habitat for birds on previously cleared farmland in south-eastern Australia

In parts of Australia, extensive areas of cleared land are now being planted with commercial plantations of native eucalypts. Questions arise about the extent to which such plantations can rectify previous loss of habitat and contribute to biodiversity conservation. This study assessed abundance of bird species (as one aspect of biodiversity) on 105 sites (25 cleared land, 58 plantations and 22 native forest) in two regions of rural Victoria, south-eastern Australia. Generalised linear modelling was used to assess some of the landscape and habitat variables that contributed to the value of plantation sites for particular groups of bird species. These models demonstrated the importance of on-site habitat variables in explaining the abundance of groups of bird species, with landscape context making small additional contributions.Mean abundance of forest and woodland birds was higher in eucalypt plantations than cleared farmland, and marginally lower than in native forest. Patterns differed between bird guilds. For example, insectivores that forage in the canopy and tall shrub layers were at least as common in plantations as in native forest, with birds in the latter group using young eucalypts as if they were tall shrubs. Birds that forage from open ground among trees were more common in plantations than native forest, and may benefit substantially from the new habitat fortuitously provided for them. This group includes several species that have declined in natural woodland habitats. Nectarivores, carnivores and birds that forage among low shrubs were less common in plantations than in native forest. Insectivores that forage from eucalypt bark made little use of plantations. Different approaches to plantation design and management would be needed to cater for groups such as these. Specific measures include planting of rough-barked eucalypts in addition to smooth-barked species, and provision of artificial hollows. Retention of existing remnants of native forest (e.g. old trees and forest patches) is a priority, to supply habitat elements that would otherwise be missing for long periods.     

不同人工造林树种及其配置方式对土壤理化性质影响分析

人工造林是黄土高原改善生态环境,减少水土流失的重要手段。不同树种及其配置方式下地表植被的生长、土壤理化性质的差异影响着生态水文功能的强弱。以长武王东沟流域8种造林树种和不同配置径流小区为研究对象,采用样方法进行造林地及林下地表植被调查,分层采样测定0—40cm土层土壤容重、孔隙度、有机质含量,分析不同人工造林模式下土壤理化性质,并初步分析了植被特征与土壤理化性质间的关系。结果表明:不同造林方式小区内林下草本层虽然覆盖度区别很大,但物种丰富度、多样性、均匀度指数差异不显著。0—20cm表层土壤理化性质变异性小于20—40cm土层。不同人工造林方式间土壤容重差异显著,且对20—40cm层土壤的毛管孔隙度、总孔隙度有显著性影响。不同造林方式下草本层丰富度、多样性指数与林下土壤毛管孔隙度相关性显著。草本层丰富度、多样性与0—20cm表层土壤的保水作用存在良好的对应关系。相比较而言,0—40cm土壤剖面上,草地和侧柏刺槐混交林地下的土壤孔隙度和有机质等理化性质,以及相关的蓄水性和入渗性等生态水文功能要好于其他造林林种。     

Conserving ground-dwelling beetles in an endangered woodland community: Multi-scale habitat effects on assemblage diversity

Patterns of biodiversity are influenced by habitat features at multiple spatial scales, yet few studies have used a multi-scale approach to examine ground-dwelling beetle diversity patterns. We trapped and quantified ground-dwelling beetle assemblages at two spatial scales: (1) microhabitat elements, represented by open ground, ground under trees and ground next to logs and (2) macrohabitat, represented by three vegetation types in a box-gum grassy woodland in south-eastern Australia. Species richness and evenness was highest at samples from under trees and lowest at samples in the open. At the macrohabitat scale, species richness and evenness did not differ among vegetation types. Assemblage composition was significantly different between trees, logs and open elements. Assemblage composition was different only between vegetation types with contrasting high and low shrub cover. Estimation of true species richness indicated assemblages at logs may have a higher number of species compared to trees and open elements, and implied greater spatial heterogeneity in assemblages at logs. Significant spatial autocorrelation in beetle assemblages was detected for logs at up to 400 m, but not for ground under trees or in the open. In agreement with previous studies, a mix of vegetation types at the macrohabitat scale is important for beetle conservation. Assemblage composition, however, appears to be more closely linked with habitat elements at the microhabitat scale, where logs support a high diversity of beetle species. This strongly supports the idea that restoring logs to box-gum grassy woodlands would be useful for increasing beetle species richness and assemblage heterogeneity.     

Positive relationship between herbaceous layer diversity and the performance of soil biota in a temperate forest

The current decline in biodiversity is particularly pronounced in the herbaceous layer of forest ecosystems. We explored the relationship between a naturally occurring plant diversity gradient in the understory vegetation of a deciduous forest and several above-and belowground ecosystem processes. We show that particularly soil microbial parameters and microarthropod densities are positively correlated with plant species richness. These results confirm recent findings in grassland ecosystems and highlight the intimate interconnectance between the diversity and functioning of above-and belowground compartments. We conclude that irrespective of a potential causal relationship between plant species richness and belowground processes, it is essential to consider the performance of soil biota in order to understand the relationship between herbaceous layer composition and ecosystem function.     

林分密度对毛乌素沙地樟子松人工林林分结构和林下植被的影响

[目的]研究不同密度下,樟子松人工林林分结构和林下物种多样性的变化,以期为毛乌素沙地以樟子松为建群种的植物固沙模式提供理论依据。[方法]以毛乌素沙地榆林地区5种不同密度25a生樟子松人工林为研究对象,采用样方调查和测试分析法,探究樟子松人工林林分结构和林下物种多样性随林分密度的变化特征。[结果]樟子松人工林密度与平均胸径呈幂函数递减关系,与高径比呈显著的线性正相关关系。樟子松人工林林下共出现植物8科13属16种。草本以禾本科的狗尾草为优势种,草本丰富度、多样性指数随着密度的不断减小,表现出明显的增加趋势。林分密度与物种丰富度指数、多样性指数呈显著的负相关关系,Shannon多样性指数对密度变化最为敏感。[结论]毛乌素沙地樟子松人工林的林分密度与林分结构、林下物种多样性的相关关系十分显著,可以将林下物种多样性指数作为衡量林分密度是否合理的参考指标。     

Grazing promotes dung beetle diversity in the xeric landscape of a Mexican Biosphere Reserve

We analyze the impact of grazing on dung beetle diversity at the Barranca de Metztitlán Biosphere Reserve, a xeric ecosystem in central Mexico with a long history of use by humans. We compared the community structure, as well as the alpha and beta diversity between two cover conditions (open and closed vegetation) that represent the impact of grazing within a habitat, and between habitat types (submountainous and crassicaule scrublands). From 576 samples we collected 75,605 dung beetles belonging to 20 taxa. While mean species richness and diversity were different between habitat types, cumulative species richness was not. The effects of grazing on vegetation structure influenced the cumulative species richness and diversity of dung beetles in the submountainous scrubland, where grazing has created land mosaics of a grassland matrix with scrubland patches. This was not the case in the crassicaule scrubland where the impact of grazing is not as evident. Beta diversity significantly responds to the effects of grazing on habitat conditions. We discuss the ecological factors that may promote these responses by landscape diversity components. We also identify the species that could act as useful indicators to monitor the effect of land management on biodiversity. Our results indicate cattle farming maintains a diversified land mosaic, and these areas support more diverse dung beetle ensembles than homogeneous areas of closed, shrubby vegetation cover. Thus, controlled grazing activity could certainly favour the conservation of dung beetle biodiversity and improve ecosystem functioning by maintaining dung decomposition rates.     

西安市城市公园树木多样性特征研究

生物多样性的维持是城市公园的一项重要生态功能,在城市环境建设和城市绿地生态效益发挥中具有重要的价值。通过对西安市21个公园40个样方的调查,发现西安城市公园共有57种树木,分属31科46属,本土树种占46.2%,国内引进种占总数的27.2%,国外引进种占总数的26.6%。所调查的21个公园物种丰富度Margalef指数为1.87,多样性指数Simpson指数为0.76,Shannon-Wiener指数为1.66,Pielou均匀度指数为0.80,表现出中等水平的多样性。西安城市公园以引进种占优势的这种树木组成,新建公园的较大树径,主要是人工配置的结果。说明社会经济的发展对城市公园的生物多样性有着重要的影响。     

Altered vegetation structure and composition linked to fire frequency and plant invasion in a biodiverse woodland

Relationships between fire history, vegetation structure and composition, and invasion by introduced plant species have received limited attention in Australian woodlands. A study in a Mediterranean, fire adapted urban Banksia woodland remnant in the biodiversity hotspot of southwest Australia investigated: (1) Have significant changes occurred in the woodland tree canopy between 1963 and 2000? (2) Do correlations exist between fire frequency and canopy cover? (3) If there is a difference in the vegetation composition of Banksia woodland invaded by the South African Ehrharta calycina (PCe) and Pelargonium capitatum (PCp) compared to largely intact remnants (GC)? and (4) Do correlations exist between vegetation condition, composition, fire frequency and invasion? Aerial photography, processed in a Geographical Information System, was used to establish fire history and changes in canopy cover over time (1963–2000). PCe and PCp sites experienced the greatest number of fires, with a net reduction in canopy cover in all areas experiencing four or more fires (60% of all woodlands). Frequent fire corresponded with a decline in native cover, richness and diversity, a shift from native to introduced species, changes in the relative importance of fire response categories, and loss of native resprouting shrub cover. Life forms of introduced species, which included no trees, shrubs and perennial sedges, contrasted strongly with those of native species, which had poor representation of annual and perennial grasses. Clear ecological and conservation consequences due to the loss of species diversity, changes in fire ecology and invasion have occurred in the Banksia woodlands. This study provides an understanding of the invasion process, enhancing conservation knowledge to improve the adaptive management of the key threatening process of invasion in biodiverse communities.     

Wood-inhabiting macrofungi in Danish beech-forests - conflicting diversity patterns and their implications in a conservation perspective

Macrofungal diversity was investigated on 281 decaying beech trees distributed across 14 forests in Denmark, based on sporocarp inventories. Two aspects of diversity were considered, i.e., species richness per fallen tree and the incidence of red-listed species occurrence per tree. For both diversity measures the effects of both tree and site variables were tested. In total, 319 fungal species were identified, including 28 red-listed. Decay stage and wood volume were identified as key variables influencing species richness as well as red-listed species incidence. Red-listed species, however, showed a preference for more decayed trees than non red-listed species. Further, red-listed species incidence was found to be significantly higher on broken trees, compared to fallen trees with a distinct root-plate, indicating tree death cause to be important for some red-listed species. The relations between diversity measures and site variables were conflicting. Species richness per tree decreased with increasing maximum tree age and dead wood continuity, possibly a consequence of competitive exclusion of unspecialised opportunistic species in old-grown stands. For red-listed species the opposite trend was evident, and it is concluded that forest history may have fundamental effects on the community structure of wood-inhabiting fungi. Accordingly, simple species richness may be a misleading conservation measure if the aim is to conserve the most threatened aspects of forest biodiversity.     

Habitat fragmentation alters the structure of dung beetle communities in the Atlantic Forest

Habitat loss and fragmentation have turned into the most important threats to biodiversity and ecosystem function worldwide. Here we investigate the effects of habitat fragmentation and drastic changes in tree communities on dung beetle richness and community structure. This study was carried out in a severely fragmented 670-km² forest landscape of the Atlantic Forest of north-eastern Brazil. Sampling was carried out in 19 forest fragments between September 2007 and March 2008 with the use of pitfall traps and flight interception traps. A total of 5893 individuals and 30 species of dung beetle were collected. Fragment area and isolation were the most significant explanatory variables for predictable and conspicuous changes in dung beetle species richness. Smaller and isolated fragments presented lower number of species, but fragments with lower tree species richness and lower proportion of shade-tolerant species were also considerably impoverished in terms of dung beetle species richness. The body mass of dung beetles were explained by fragment area and the percentage of emergent trees with smaller and less stratified fragments being dominated by small-bodied dung beetles. An ordination analysis segregated dung beetle communities between small fragments (<100 ha) and the control area. Seventy-seven percent of the species were recorded in the control area and 22% of all species were unique to this habitat. Our findings indicate that large fragments in the Atlantic Forest appear to consist in a sort of irreplaceable habitats for particular groups of dung beetle species, as well as for the integrity of their communities.     

Management strategies to increase stand structural diversity and enhance biodiversity in coastal rainforests of Alaska

Coastal rainforests of southeast Alaska have relatively simple species composition but complex structures with high diversity of tree ages, sizes and forest canopy layers, and an abundant understory plant community. Wildlife and fisheries resources also play an important role in the ecological functioning of forest and aquatic systems. Clearcutting has greatly altered these forest ecosystems with significant decreases in structural diversity of forest stands and greatly reduced wildlife habitat. This paper synthesizes information on management options in older forests that have never been actively managed, and in younger forests to increase diversity of stand structures and their associated effects on biodiversity. Light to moderate levels of partial cutting in old-growth forests can maintain the original diversity of overstory stand structures and understory plant communities. In younger forests that develop after clearcutting, mixed alder-conifer stands provide more heterogeneous structures and significantly higher understory biomass than in pure conifer forests. Research has shown that red alder increases diversity and abundance of understory plants, and provides forage for deer and small mammals. Results also show a clear linkage between alder and improved invertebrate diversity in aquatic systems. A combination of light partial cutting in older forests along with inclusion of red alder in conifer-dominated forests could provide the greatest amount of diversity and maintain the complex stand structures that are an important component of these forest ecosystems.     

Maximising biodiversity in plantation forests: Insights from long-term changes in clearfell-sensitive beetles in a Pinus radiata plantation

Forest management practices can be detrimental to the long-term persistence of native species in exotic tree plantations. Knowledge of population recovery times post-harvest and dispersal capabilities of native species are critical to the development of spatially-explicit stand management plans that maximise biodiversity opportunities at a landscape scale. To quantify post-harvest recovery patterns we studied the relative capture rates of seven beetle species known to be sensitive to clearfell harvesting. A chronosequence sampling approach was implemented, with sampling along 580-m long edge gradients from the interior of multiple replicated mature stands of 26 years of age into adjacent regenerating stands of 1, 2, 4, 8, 16, and 26 years of age. Beetle capture rates were strongly affected by clearfell harvesting, however, population recovery of all species was possible within the timeframe of a single rotation when adjacent mature or closed-canopy stands were present. Recovery time was closely linked to the development of a closed canopy (8–16 years), with distinct differences in the responses of individual beetle taxa reflecting habitat preferences for open or closed forest. Such time-to-recovery analyses provide insights into temporal aspects of insect–plant–habitat interactions and can give forest managers better guidelines to maximise biodiversity opportunities at the landscape scale. Although the time to canopy closure provides a useful proxy for recolonisation by forest species, specific timeframes will vary biogeographically and are dependent on the original native vegetation context and species assemblages present. In addition, forest managers and public advocacy groups will need to make qualitative decisions about what biodiversity attributes should be conserved.     

The value of primary, secondary and plantation forests for Amazonian birds

Secondary forests and exotic tree plantations are rapidly expanding across tropical landscapes, yet we currently have a very poor understanding of the value of these human-dominated forest landscapes for biodiversity conservation. Mist netting, point counts and transect walks were used to compare the bird communities of these habitats and neighboring primary forest in north-east Brazilian Amazonia. The extensive spatial scale of plantations and second-growth in our study area enabled us to implement a robust replicated design, with survey plots approximately two to three orders of magnitude larger than most previous studies of land-use change in the tropics, thus minimising the influence of the surrounding landscape. Species richness was highest in primary forest and lowest in Eucalyptus plantations, and community turnover between habitats was very high whether based upon matrices of relative abundance or species presence-absence data, and for both point count and mist net data. Monthly line-transect censuses conducted over an annual cycle showed an increase in the detection of canopy frugivores and seed predators during the peak of flower and fruit availability in primary forest, but failed to suggest that second-growth or Eucalyptus stands provide suitable foraging habitat at any time of the year. The conservation value of both secondary forest and plantations was low compared to conclusions from previous studies. Our results indicate that while large-scale reforestation of degraded land can increase regional levels of diversity, it is unlikely to conserve most primary forest species, such as understorey insectivores and canopy frugivores.     

The effects of mixed broad-leaved trees on the collembolan community in larch plantations of central Japan

The effects of broad-leaved trees on the collembolan community in larch plantations were investigated at the foot of Mt. Yatsugatake (1200–1400 m a.s.l.) in Japan. The study sites comprised five pure larch plantation plots (larch dominated more than 95% of the area at breast height) and five mixed forest plots (larch dominated between 50% and 80% of the area at breast height). We compared the collembolan community structures between stand types and related them to the plant community composition and soil properties at each plot. Density and species richness of Collembola were not significantly different between pure larch and mixed plots. Using partial redundancy analysis (pRDA), the variance of collembolan species data in the litter layer was explained by the biomass of grass on the forest floor, and the variance in the soil layer was explained by the biomass of total forest floor plants. These results suggest that the biomass or the composition of forest floor plants influence the collembolan community more than the crown trees in this area.     

Local and landscape factors determine functional bird diversity in Indonesian cacao agroforestry

Large-scale intensification of smallholder cacao management is currently affecting the agroforestry landscapes of Sulawesi (Indonesia), the world’s third largest cacao producer. Little is known about how this shift from diverse plantations to full-sun cacao will affect functionally important biodiversity within the agroecosystem, and how this is related to landscape-wide patterns in land-use and natural ecosystems. We recorded birds in 43 cacao plots differing in woody and herbaceous vegetation as well as distance to forest in two valleys around the Lore Lindu National Park in Central Sulawesi. Species richness of frugivores and nectarivores decreased with increasing distance to forest, whereas granivorous birds increased in richness. Forest specialists, but not habitat generalists, responded positively to forest edge proximity. Species richness of all functional groups except seed eaters increased at higher density of tall shade trees. Greater species richness of shade trees was associated with higher species richness of frugivores and nectarivores, while herbaceous vegetation did not have a strong impact on the avifauna. The positive effect of shade trees was independent of distance to forest. In conclusion, our study shows the relative importance of local and landscape effects on bird diversity with shade trees being critical for bird conservation in cacao agroforestry landscapes.     

Biodiversity and ecosystem function of tropical forest dung beetles under contrasting logging regimes

A key challenge for tropical conservation biologists is to assess how forest management practices affect biodiversity and associated ecosystem functions. Dung beetles (Scarabaeidae: Scarabaeinae) provide an ideal focal guild for such studies. We compared dung beetle assemblages and experimentally assessed rates of dung removal and seed burial in undisturbed forest, low-intensity selectively logged forest under sustainable forest management, and high-intensity logged forest, not under sustainable management in Malaysian Borneo. In total, 7923 individuals from 39 species of dung beetle were collected. There were no significant differences in abundance, biomass or diversity across sites. Species richness was significantly lower in the high-intensity logged sites. Beta diversity was low: of 35 species recorded in undisturbed forest sites, 32 were also captured in low-intensity sites and 29 in high-intensity sites. The environmental and vegetation variables measured had little influence on species composition. An average of 63–99% of dung was removed over 24 h. Mean dung and seed removal were significantly lower in the high-intensity logged sites. Dung removal rates were significantly and positively correlated with dung beetle species richness, but not with dung beetle biomass or abundance. However, the biomass of large-bodied, nocturnal dung beetles was positively correlated with dung removal. In contrast to previous studies, dung beetle biomass and abundance were not correlated with species richness, indicative of density compensation. Overall, dung beetle communities and associated ecosystem functions were robust to low-intensity but not high-intensity selective logging. These differences may be related to changes in the abundance and biomass of particular dung beetle species or guilds rather than community-wide measures of abundance and biomass, highlighting the need to move beyond simplistic biodiversity-ecosystem functioning correlations to understand the functional consequences of habitat modification in high-diversity ecosystems.     

Changes of the functional diversity of soil microbial community during the colonization of abandoned grassland by a forest

The impact of secondary succession of grassland communities towards a Norway spruce forest on soil microbial community was studied on a belt transect established in the Pol’ana Mts., Central Europe. Data on understory vegetation, light availability, soil properties and microbial activity were collected on 147 plots distributed over regular grid. Moreover, distributions of functional groups of microorganisms were assessed using BIOLOG analysis on a subset of 27 plots. Mantel partial correlations between microbial community indicators and environmental variables showed that microbial activity generally decreased with increasing tree density and size, whereas it increased with increasing radiation at the soil surface, soil temperature, and cover and diversity of understory vegetation. Functional richness and diversity of microorganisms were positively correlated with solar radiation, but also with plant species richness and diversity. Abundance of several functional groups correlated closely with succession-related variables. Redundance analysis of microbial data provided slightly different outcomes. Forward selection yielded only two environmental variables significantly influencing the composition of the microbial community: tree influence potential and organic carbon content. Abundances of several functional microbial groups correlated with tree influence, documenting that microbial community changes are at least partially driven by the colonization of grassland by trees. Nevertheless, the relative importance of abiotic environment change and plant community succession on microbial community dynamics remains unresolved.