Nonsurgical removal of urethral uroliths using a self-retaining retractor with elastic stays in female guinea pigs (Cavia porcellus): 16 Cases (2006–2019)

Sixteen cases of client-owned female guinea pigs (Cavia porcellus) involved urethrolith removal directly from the urinary orifice or urethra with the aid of a lightweight self-retaining retractor ring with elastic stays. The procedure was performed in fifteen guinea pigs and repeated once due to reoccurrence of urolith formation. Urethrolithiasis was diagnosed by direct palpation of the urethrolith, and in all but one case confirmed radiographically. Uroliths were removed whole or in sections after direct visualization with the help of the retractor. Only minor complications associated with the procedure were observed and most patients survived to discharge. This is the first case series to describe the use of a self-retaining retractor to facilitate simple nonsurgical removal of urethroliths in female guinea pigs. This procedure is rapid, minimally invasive, technically simple, and cost effective in female guinea pigs with uroliths in the urinary orifice or distal urethra.     

Equus caballus papillomavirus‐2 (EcPV‐2): An infectious cause for equine genital cancer?

Reasons for performing study: The aetiology of genital squamous cell carcinoma (SCC) in horses remains unknown, but the similarity to the disease in man, for which papillomavirus infection has been shown to be a causal factor, requires to be investigated in horses. Hypothesis: One or more novel papillomaviruses cause equine genital SCC and its associated premalignant lesions. Methods: DNA was extracted from samples of equine genital SCC and performed rolling circle amplification, in order to identify closed circular DNA viral genomes within the samples. The amplified DNA was subcloned and sequenced and the DNA sequence compared to that of other papillomavirus genomes. Using PCR primers developed from these genomic DNA sequences, studies were then carried out in order to identify the frequency at which the viral DNA could be identified in equine genital cancer samples from horses in both the UK, Australia and Austria. Finally, in situ hybridisation using specific probes developed from this DNA sequence were used to confirm the presence of the viral RNA sequences in the neoplastic cells in these lesions. Results: The full length genome of a novel papillomavirus species was characterised from the equine genital SCC tissue and termed Equus caballus papillomavirus‐2 (EcPV‐2). Viral DNA and RNA was identified in the genital tumour samples, but not in the adjacent histologically normal tissue. EcPV‐2 DNA could not be identified in equine ocular or nasal carcinomas or within the scrotal skin or in most smegma samples obtained from tumour‐free horses. Sequencing of amplicons, generated from the archived equine genital tumours, identified variations within E1 and E6 on DNA and predicted protein level. Conclusions: A novel papillomavirus, EcPV‐2, is likely to play a causal role in the pathogenesis of equine genital epithelial tumours. Potential relevance: Identification of a papillomavirus causal for genital carcinomas in horses may lead to development of a vaccine that could be used to prevent this serious disease in horses. This would be analogous to man, where vaccination against oncogenic papillomavirus species is currently being used to help prevent cervical cancer.     

Infection level of monogenean gill parasite, Diplozoon kashmirensis (Monogenea, Polyopisthocotylea) in the Crucian Carp, Carassius carassius from lake ecosystems of an altered water quality: What factors do have an impact on the Diplozoon infection?

Monogeneans are of great interest to the ecologists because of their simple life cycle and are considered as one of the important and sensitive parasites to any changes in water quality. The monogenean gill parasite, Diplozoon kashmirensis of the Carassius carassius was examined with the aim to evaluate the infection level and the factors influencing the infection. Our results showed that highest prevalence (34.22%) of D. kashmirensis was in the lake having high trophic status and least prevalence (10.90%) in the lake having least trophic status. Infection levels were significantly higher at the basins/sites having deteriorated water quality in comparison to the basins/sites having better water quality. The combined effect of pollutants and eutrophication showed decrease in intensity of D. kashmirensis in one of the polluted/hypertrophied site in the hypertrophic lake (Anchar Lake), while as synergistic effect (i.e. increase) on prevalence and intensity was observed in one of the polluted/hypertrophied site in the eutrophic lake (Dal Lake). Sex was not an important factor influencing the D. kashmirensis in three lakes. The study showed that condition factor was significantly higher in the hypertrophic lake and in an uninfected fish. In addition microhabitat influenced the Diplozoon infection. Most preferable site for the D. kashmirensis was middle sector of the gill. Furthermore, the gill parasite showed significantly positive correlation (r(p)=0.6, P<0.05) with water temperature in the hypertrophic lake, thus showing the impact of water temperature on this parasite. It was concluded that intra lake differences, season, condition factor, microhabitat and water temperature influenced the infection of D. kashmirensis. It seems from the data that eutrophic and hypertrophic habitats were favourable for the D. kashmirensis. The D. kashmirensis showed both antagonistic and synergistic response to the combined effect of pollution and eutrophication.