Forcing vine regrowth and shifting fruit ripening in a warm region to enhance fruit quality in ‘Cabernet Sauvignon’ grapevine (Vitis vinifera L.)

Summary

In warmer regions, winegrapes ripen their fruit during the hottest portion of the growing season, producing wines of high pH, low acidity, less intense colour, and a less complex flavour. Experiments were conducted in a commercial vineyard of ‘Cabernet Sauvignon’ at Fresno, CA, USA in 2009, and in 2010, to determine if vines could be forced to enhance fruit quality. Forcing was conducted by hedging growing shoots to six nodes and removing summer laterals, leaves, and primary clusters between 14 – 70 d after anthesis in late-May, June, and July. Vines grown under conventional practices were used as controls. Forcing in June shifted fruit ripening from the hot (July and August) to the cool (October through early-November) portion of the growing season, a period with more hours at lower, more favourable temperatures. Fruit from the forced crop had smaller berries, a lower pH, higher acidity, and higher contents of anthocyanins, tannins, and total phenolics, compared with non-forced fruit. The yield and vigour of forced vines were comparable to, or slightly lower than, control vines. The best forcing treatment consisted of hedging growing shoots to six nodes and removing summer laterals, leaves, and primary clusters. Our study demonstrates the potential of forcing to address the detrimental effects of high temperatures on fruit quality in warmer regions of winegrape production.     

Effects of crop load and time of thinning on the incidence of split pits,fruit yield,fruit quality,and leaf mineral contents in ‘Andross’ peach

Summary

The effects of light, moderate, or heavy thinning (5, 10, or 20 cm between fruit, respectively), conducted before (7 d or 15 d), during, or after (7 d or 15 d) pit hardening (PH), on the incidence of split pits, fruit yield, fruit quality characteristics, and leaf mineral contents, were studied in the canning peach (Prunus persica L. Batsch.) cultivar ‘Andross’ over two growing seasons. The percentage of fruit with split pits increased by 58.2% in heavily-thinned trees compared with moderately- or lightly-thinned trees, and by 22.9% for the earliest time of thinning (15 d before PH) compared with thinning during, or after PH. Fruit fresh weight (FW) was greater in moderately- and heavily-thinned trees compared with lightly-thinned trees, but yields were similar among the different crop-load treatments. The latest time of thinning (15 d after PH) also had a negative impact on yield at first harvest and on total yield, fruit FW, and delayed fruit ripening compared to thinning during, and 15 d before PH. Total anti-oxidant capacities and phenolic contents were usually greater in fruit from heavily-thinned compared with lightly- or moderately-thinned trees only when thinning was conducted during, or 15 d after PH. Moreover, anti-oxidant levels were highest in fruit from the earliest-thinned trees. There was no significant effect of crop load, or of time of thinning application treatment on fruit colour, or on the K, P, Fe, Mn, and Cu contents of leaf tissues. In conclusion, light or moderate thinning during PH resulted in minimal split pits during processing, and in optimal yields and fruit quality characteristics in the canning peach cultivar ‘Andross’.     

Dried fruit and its functional properties from a consumer’s point of view

Summary

Modern drying technology provides the opportunity to obtain dried fruits with high concentrations of bioactive compounds. Such products may also be fortified with functional ingredients. The adoption of dried fruit as a carrier of functional ingredients for consumers is indispensable to launch this kind of product successfully on the market. Thus, the aim of this study was to collect data on consumer perceptions and interests in dried fruits (plain or fortified) through a questionnaire distributed on the Internet to 1,092 Dutch, French, and Polish respondents. In this quantitative study, the respondents were first asked to rank statements about a product with different positive influences on human health. Products which lowered the risk of cancer or heart diseases were mainly of interest to Polish and Dutch respondents, whereas French consumers emphasised the prevention of intestinal problems. Furthermore, we checked the level of consumer interest in dried fruits enriched with a particular functional ingredient (e.g., anti-oxidants, natural fruit sugars, or prebiotics). Products with anti-oxidants seemed to be the most promising in all three countries investigated. Among five different forms of product (i.e., candy, fruit teas, cereals, bars, or cookies) in which dried fruit could be incorporated, cereals were selected by approx. 33% of all respondents as the best product to which a functional dried fruit could be added. In summary, dried fruits can be adopted as carriers of functional ingredients, especially when promoted as a source of anti-oxidants.     

Improvement of fruit quality and storage potential of ‘Braeburn’ apple through deficit irrigation

Summary

We explored the potential of deficit irrigation (DI) applied at different times during the growing season for improving fruit quality and storage potential of ‘Braeburn’ apple growing in a commercial orchard. Because DI often reduces fruit size, effects of fruit size on fruit quality were also examined. The irrigation treatments were: commercially irrigated control (C), early deficit irrigation (EDI) applied from 63 to 118 d after full bloom (DAFB), and late decifit irrigation (LDI) applied from 118 DAFB to final harvest on 201 DAFB. Both EDI and LDI improved fruit quality in terms of increases in: dry-matter concentration (DMC) at harvest, flesh firmness, total soluble solids, and total soluble sugars both at harvest and after storage. The DI fruit had less weight loss during storage than did C fruit. Deficit irrigation affected the concentrations of a few individual aroma volatiles but not total volatile concentration. Incidence of physiological disorders was not affected by irrigation treatments. Mean fruit weight was lower in DI than in C but the difference was not significant. Among the quality attributes studied, only firmness and DMC were affected by fruit size with their values being higher in smaller fruit.     

Determination of optimal harvest boundaries for ‘Ambrosia’ apple fruit using a delta-absorbance meter

In this study, a new chlorophyll measurement tool, the delta absorbance (DA) meter, was used to develop an optimal harvest maturity model for ‘Ambrosia’ apple (Malus × domestica Borkh.) fruit. Fruit from four commercial orchards in the Annapolis Valley, Nova Scotia, Canada, were sampled (25 fruit from three or four trees per location) over nine consecutive weekly harvests during the 2011 and 2012 growing seasons, and 8 weeks in the 2013 season. At each harvest, five fruit from each orchard site had their index of absorbance difference (I_AD) values, firmness, mass, titratable acidity (TA), soluble solids content (SSC), red skin colouration and internal core ethylene concentrations measured. Following approx. 3 months of storage at 3.5°C, 20 fruit from each site were removed and assessed for the incidence of disorders such as senescent breakdown, cortical browning and coreflush. Chlorophyll concentrations in the epidermis were strongly and positively related to I_AD values in the same tissue (P ≤ 0.001), confirming the assumption that chlorophyll was the basis for the DA meter I_AD signal. In addition, I_AD values declined significantly during fruit maturity and were negatively related to harvest week (P ≤ 0.001). The optimum harvest period was identified by aligning all ‘at harvest’ I_AD values, fruit quality measurements, and ‘post-storage’ disorder data with the corresponding harvest week. I_AD values associated with harvests having the highest commercial fruit quality then delineated the optimal harvest boundaries. The upper boundary I_AD value of 0.47 was defined as ‘when to begin harvest’, while the lower boundary I_AD value of 0.28 was considered to be ‘when to end harvest’ for long-term storage. The use of a DA meter and its I_AD value to define the optimal harvest boundaries may be applicable to all commercial apple cultivars, but should be developed for each cultivar and growing region.     

Postharvest handling practices and irradiation increase lenticel discolouration in ‘B74’ mango fruit

‘B74’ mango is a recently commercialised cultivar in Australia, with an appealing skin colour and firm fibreless flesh. However, fruit can develop lenticel discolouration (LD) after harvest, with loss of commercial value, especially after γ-irradiation as a disinfestation treatment. We hypothesised that postharvest practices could increase fruit sensitivity to LD and tested that by sequentially sampling fruit between the orchard and the end of the packing line over two seasons, followed by ripening without and with irradiation treatment. Exposure of 441–610 Gy γ-irradiation significantly increased the severity of LD by 6.8-fold in commercially picked and packed ripe fruit, reducing the proportion of marketable fruit from 98% to 2%, compared to irradiated fruit harvested directly from the trees and not exposed to de-sapping solution and packing operations. Also, LD increased progressively as the fruit passed through the harvesting and packing processes, and exposure to only bore water increased LD severity compared with no water contact. Results suggest that the typical de-sapping process used during harvesting is a major contributor to skin sensitivity to LD in ‘B74’ mango fruit, and that other packing operations involving wetting of the fruit have an additive effect on it. These effects are exacerbated if fruit is irradiated.     

The effect of gibberellins and cytokinins on fruit development of Bramley’s Seedling apple

Two applications of a 1 : 1 mixture of gibberellins A₄ and A₇ to either frost damaged or artificially decapitated Bramley's Seedling apple flowers resulted in increased fruit set and yield. It also increased the crop in an orchard without pollinators. Gibberellic acid was not effective. The addition of cytokinins had no beneficial effects and caused malformation.     

Aroma volatiles emission in relation to chilling injury in ‘Kensington Pride’ mango fruit

Summary

Mature green mango fruit (Mangifera indica L. ‘Kensington Pride’) were stored at 0, 5, 10, 15 or 20°C for 1, 3, 7, 14, 21 or 28 d to induce different levels of chilling injury (CI) and to elucidate its relationship with aroma volatile production. The fruit were removed from storage and allowed to ripen at 22 ± 1°C and CI index was assessed on fully ripe fruit. Aroma volatile compounds were estimated from the pulp of fully ripe fruit. CI index significantly increased as the storage temperature was lowered and the storage period was extended, particularly in the fruit stored at 0, 5 or 10°C. CI symptoms did not develop on fruit stored at 15 or 20°C. Fifty-six aroma volatile compounds were identified from mango fruit pulp using headspace solid phase microextraction (SPME) technique with gas chromatography (GC-FID) and GC combined with mass spectrometry (GC-MS). Among the 56 aroma volatile compounds, 25 were quantified using GC, which included monoterpenes (±-pinene, ²-pinene, myrcene, 2-carene, ±-phellandrene, 3-carene, ±-terpinene, limonene, ocimine, ³-terpinene, ±-terpinolene, and a-terpineol), sesquiterpenes (±-copaene, ±-gurjunene, trans-carophyllene, aromadendrane, ±-humulene, alloaromadendrane, ³-gurjunene, and ledene), hydrocarbon (p-cymene), esters (methyl octanoate and ethyl caprylate), aldehyde (decanal), and norisoprenoid (²-ionone). A significant reduction in total aroma volatiles, monoterpenes, sesquiterpenes, hydrocarbon, esters, aldehyde, and norisoprenoid production were observed in fruit stored at 0, 5, 10 or 15°C compared with fruit stored at 20°C. The degree of reduction in aroma volatile compounds depended on the severity of CI, induced with different storage temperatures and storage periods. In conclusion lower temperature storage induced CI in mango fruit and adversely affected the aroma volatiles production.     

Progress in research on meteorological disaster indicators of major fruit trees in China北大核心CSCD

China is an important fruit-producing country in the world. In recent years, the fruit planting area in China increased significantly, and the total fruit output has also rapidly increased. In this context of development, the characteristic fruit industry has become an important source of rural agricultural economic income in China. The stable production of characteristic fruits and disaster prevention and mitigation are of great significance for improving people's living standards and promoting the development of agricultural economy. At the same time, China has a vast territory, spanning three main climatic zones from north to south, and the damage caused by meteorological disasters of fruit trees is extremely serious and frequent. Therefore, research on meteorological disasters of fruit trees is one of the hot topics in agrometeorological disasters research. Especially, the research on meteorological disaster indicator of economic fruit trees is particularly important, which is an inevitable link in disaster monitoring, early warning, defense, planting planning, risk zoning and other research. In this paper, the main meteorological disaster indicators of fruit trees in China are classified and systematically expounded, by using the method of classification and summary. In this paper, the basic concept and significance of meteorological disaster indicators of fruit trees are defined firstly, and then the research history and progress of meteorological disaster indicators of fruit trees in China are summarized. The main research methods of meteorological disaster indicator of fruit trees in China, including fruit trees and disaster types, technology level and main achievements, were summarized in two periods before and after the 21st century. On this basis, the existing meteorological disaster indicators of fruit trees in China are divided into three categories according to their construction methods: the empirical indicator construction method based on disaster verification and disaster investigation; the comprehensive indicator construction method based on the relationship between disaster-causing factors and yields; and the experimental construction method based on artificial climate chamber or artificial climate box. According to the composition types of meteorological disasters of fruit trees in China, the existing meteorological disasters indicators of fruit trees are divided into three categories: single factor indicators, multi-factor indicators and comprehensive indicators. According to the key meteorological factors causing meteorological disasters of fruit trees, the existing meteorological disasters indicators of fruit trees are divided into several categories: low temperature disaster indicators, high temperature disaster indicators, drought disaster indicators, rainstorm disaster indicators and so on. Secondly, aiming at the northern fruit trees, this paper summarizes the research on late frost damage indicator of apple, pear, apricot, plum and grape in spring, drought disaster indicators of apple and walnut, hot damage indicators of apple and kiwifruit in summer, rainstorm disaster indicator of apple and jujube in autumn, and freezing injury indicators of pear, apricot and jujube in winter. For southern fruit trees, it summarizes the research on high temperature damage indicator of citrus, and the research on freezing damage indicator of citrus, litchi, longan, mango and loquat in winter. At the same time, this paper summarizes the types, construction methods, uses, advantages and disadvantages of the above meteorological disaster indicators of fruit trees in agrometeorological service. Finally, this paper discusses the existing problems and the future development direction of the research on meteorological disaster indicators of fruit trees, from the aspects of indicator composition, indicator construction method, fruit tree species, industrial development needs, and new technology and methods. It is expected that the results of this study can provide reference for the distribution of main fruit trees, industrial optimization, disaster prevention and mitigation, and provide scientific guarantee for the healthy, stable and sustainable development of fruit tree growing industry in China. © 2019 Journal of Fruit Science     

The effects of hydrogen cyanamide on budburst and fruit maturity of ‘Thompson Seedless’ grapevine

Summary

The effect of hydrogen cyanamide (H₂CN₂) on budburst and fruit maturity of `Thompson Seedless' grapes was examined. Nine year old vines of cv. Thompson Seedless grafted on R.110 rootstock and supported on overhead tendone trellis (parral), were treated with either 2% or 4% of H₂CN₂, 53 and 67 d before expected budbreak and compared with unsprayed vines. The earliest treatment with 4% H₂CN₂ enhanced budbreak and advanced fruit maturity by 17 d. However, the same concentration applied 53 d before natural budbreak advanced the date of maturity by only 4 d. All the treatments increased significantly the percentage of budburst compared with the control. H

² CN₂ had no significant effect on yield, but a positive effect on fruit quality. A higher index of maturity was obtained by the treatment: 4% H₂CN₂, 67 d before budbreak. All the treatments of H₂CN₂ induced more uniform budburst compared with the control. The best uniformity was obtained by the earliest spray using the higher concentration (4%).     

Effect of calcium supply on the physiology of fruit tissue in ‘Andesu’ netted melon

Summary

Fruit development and physiological traits were evaluated in ‘Andesu’ netted melon plants grown with and without calcium. Calcium exclusion accelerated softening, alcoholic fermentation and ethylene evolution of fruit compared with those supplied with calcium, but the opposite trend occurred with sucrose accumulation. A significant difference of calcium concentrations in the fruit between treatments was observed at the developing stage of the fruit (P<0.05), but the difference became smaller as fruit matured. In the mesocarp tissue from the basal hemisphere, calcium concentrations in the NaCl soluble fraction at ripe fruit stage differed significantly (P<0.05) between calcium treatments but no significant difference was detected in the inner mesocarp of the distal hemisphere. Calcium exclusion did not lead to the development of water-soaked symptoms in the mesocarp tissue of ripe fruit. Uronic acid concentrations in ionically bound pectin fraction did not differ between calcium treatments throughout fruit development. In covalently-bound pectin fraction, however, uronic acid concentration of ripe fruit was significantly lower in calcium-excluded plants than in calcium-supplied plants (P<0.05). Our results suggest that: first, accelerated fruit softening under calcium deficient conditions might result from promoted ethylene evolution rather than from the shortage of ionically-bound calcium in cell walls, second, calcium deficient condition does not necessarily lead to water-soaked symptoms in tissue of ‘Andesu’ melon fruit.     

Influence of rootstock on orchard productivity and fruit quality in peach cv. ‘Redhaven’

Summary

We have evaluated the influence of four different peach rootstocks (Prunus pumila,‘GF 655/2’ ,‘Missour’ and ‘GF 677’ as a standard) planted in medium-heavy to heavy soil on phenological events (flowering and harvest date), growth, yield, fruit quality and mortality of peach (P. persica L.) scions of cv. ‘Redhaven’. At the end of flowering, there were no significant differences between the different rootstocks in the years 1999, 2000, 2003 and 2004. In 2001, trees grafted on P. pumila reached the end of flowering 2 d before trees grafted on the other rootstocks. ‘Redhaven’ fruits ripened on the same day on trees grafted on the different rootstocks. The differences were only between years. The results showed that significantly less vigorous trees, according to their trunk cross-sectional area (TCSA) and canopy volume, with the lowest yield, were observed on P. pumila rootstock. The rootstock P. pumila had a significant negative influence on fruit dimensions and mortality was 50%. Trees grafted on ‘GF 655/2’ rootstock produced significantly lower yields than those grafted on ‘GF 677’ or ‘Missour’ rootstocks. Only the ‘GF 655/2’ rootstock had root suckers. Trees grafted on ‘GF 677’ rootstock were significantly more vigorous than on the other rootstocks, and produced the highest yield with good fruit quality. The final canopy volumes of peach trees grafted on the different rootstocks were very different. The most vigorous trees were those grafted on ‘GF 677’ rootstock, where the final tree volume was 12.8 m³. Trees grafted on P. pumila and ‘GF 655/2’ had significantly lower TCSA values than on the other rootstocks.     

Response of the phenylpropanoid pathway to Venturia inaequalis infection in maturing fruit of ‘Braeburn’ apple

Summary

This study investigated the activities of key enzymes in the phenylpropanoid pathway and the accumulation of phenolic products in apple peel in response to infection with Venturia inaequalis [(Cooke) G. Wint.]. We compared healthy apple peel with apple peel showing symptomatic scab lesions, and with peel tissue from 1 – 2 mm around the scab lesions in fruit 1 month before maturity [140 d after full bloom (DAFB)] and at physiological maturity (175 DAFB). Infection with V. inaequalis enhanced the synthesis of some phenolic compounds. Compared to healthy peel, scab lesion tissue had ≤ 3.1-times higher hydroxycinnamic acid content, ≤ 1.3-times higher dihydrochalcone content, and ≤ 3.9-times higher flavan-3-ol content. Scab lesions showed slightly higher phenylalanine ammonia-lyase, chalcone synthase, chalcone isomerase, flavonol synthase, and dihydroflavonol 4-reductase activities. The total amount of phenolics remained relatively stable between the two sampling dates, except for epicatechin and caffeic acid which decreased in amount toward fruit maturity, and levels of catechin which increased in more mature fruit. During fruit maturation, only chalcone synthase and chalcone isomerase activities decreased in all tissues examined. This study showed that the phenylpropanoid pathway in apple fruit peel changed significantly following infection with the apple scab pathogen.     

The effect of selective picking on the harvest fruit size distribution of ‘Royal Gala‘ apples

Summary

The size distribution of fruit on an apple tree on any potential harvest date can be estimated by measuring a sample of fruit early in the season and projecting forward in time using standard growth curves. However, ‘Royal Gala‘ apples grown in New Zealand are picked in multiple harvests spread over about three weeks, so the overall size distribution of harvested fruit can differ significantly from that on any single harvest date. On each havest date, fruit are selected for picking according to background colour, not size. By measuring the sizes at harvest of all fruit on selected limbs of eight ‘Royal Gala‘ trees in Hawkes Bay, New Zealand, we show that the relationship between the size distributions of picked and unpicked fruit can be described by a single-parameter model. Although size is not used directly in fruit selection, we find that larger fruit still tend to be picked first, with the odds of a fruit being selected approximately doubling for each 20.g increase in size. The mean harvest fruit size is largest for the first harvest, and decreases in later harvests, despite ongoing fruit growth. Because the smaller fruit left on the trees continue to grow, the standard deviation of the overall fruit size distribution from multiple harvests can be expected to be significantly smaller than it would have been had all fruit been harvested on the same date. The model is tested using size and background colour measurements collected on tagged fruit at three sites in two years, including a range of shading and crop load treatments. For most combinations of site, year and treatment, the model predicted the size distribution of picked fruit well, but in a few cases a different parameter value was need to obtain a good fit.     

Post-harvest putrescine treatments extend the storage-life of apricot (Prunus armeniaca L.) ‘Tokhm-sefid’ fruit

Summary

The aim of this study was primarily to increase the storability of apricot which is normally a very short storage-life stone fruit. Polyamines are well-known to improve the storability of many horticultural crops. The effect of exogenously applied putrescine on the post-harvest storage-life of apricot (Prunus armeniaca L.) ‘Tokhm-sefid’ fruit at 2°C was therefore investigated. The rate of ethylene production, fresh weight (FW) loss, tissue firmness, soluble solids content (SSC), titratable acidity (TA), and the pH of fruit were determined 5, 10, 15, 20 and 25 d after the beginning of storage. Overall, the application of putrescine caused a reduction in ethylene production, as well as an increase in fruit flesh firmness. SSC and pH were reduced, and TA was increased in putrescine-treated fruits. The loss of fruit FW was affected by putrescine in a concentration-dependent manner. Thus, fruit treated with higher concentrations of putrescine showed lower FW loss.     

Physiological responses and quality attributes of Japanese pear ‘Kosui’ fruit kept in low oxygen atmospheres

Summary

Japanese pear ‘Kosui’ fruits were stored under a continuous flow of 0%, 1%, 3%, 5% and 10% O₂ (balance N₂) or air for 7 d at 20°C to study the effects of low O₂ on their physiological responses and quality attributes. Low O₂ treatments did not significantly influence changes in skin colour and soluble solids content. However, weak off-flavours were detected in the fruits stored at 0% O₂ on day 3, and the intensity of these off-flavours increased as storage progressed. The concentrations of acetaldehyde in fruit increased throughout the storage period. The ethanol concentration was greatly increased in fruits stored at 0% O₂. Moreover, ethanol concentrations were much higher than those of acetaldehyde and remained very low during storage in air, but their concentration were just slightly increased in fruits exposed to 1%, 3%, 5% and 10% O₂. Pyruvate decarboxylase activity was greatly increased in fruits exposed to 1% and 3% O₂, while its activity in fruits exposed to 5% and 10% O₂ were only slightly higher than that of the control and at 0% O₂ at the same level as the control. Alcohol dehydrogenase (ADH) activity greatly increased in fruit exposed to 0%, 1%, 3% and 5% O₂, while at 10% O₂, ADH was only slightly higher than the control. Changes in ADH isozymes correlated well with changes in ADH activity. The homogenate pH of fruits exposed to 1%, 3%, 5% and 10% O₂ and air remained constant, while in fruit stored at 0% O₂ their pH increased. The potential for using low O₂ atmospheres to help in maintaining the quality of Japanese pear ‘Kosui’ is discussed.     

Comparison of sampling strategies for determining fruit mineral concentrations in ‘Hass’ avocado (Persea americana Mill.)

Summary

Spatial and temporal variation in mineral and dry matter (DM: % dry weight) concentrations were examined in ‘Hass’ avocado (Persea americana Mill.) fruit to identify sampling strategies for examining fruit mineral, DM and fruit quality relationships. Calcium (Ca), magnesium (Mg), potassium (K), and DM concentrations were compared from different parts of the same fruit, from fruit harvested from different shoot types and locations within the same tree, and at different stages during the growing season, from fruit set to commercial harvest. The main source of variation within fruit was longitudinal, with K and DM concentrations being higher at the distal end of the fruit. Sampling methods based on equatorial plugs of tissue provided the best estimates of whole fruit values for DM and mineral nutrients. While there was much variability in the internal distribution of minerals within the fruit, the distribution was the same for fruit from both the northern and southern aspects of the tree. During the first 4 months after fruit set, fruit from determinate floral shoots (i.e., with a flower in the terminal position) had higher Ca and Mg concentrations than fruit from indeterminate shoots (i.e., with a shoot in the terminal position). After this time, fruit Mg and Ca concentrations were higher on indeterminate shoots, possibly because of changes in sink-strength and increased transpirational flow associated with new leaf growth on indeterminate shoots. The data showed that shoot type was a source of fruit-tofruit variability in an avocado crop, and that care should be taken in sampling fruit tissues for nutrient analyses.     

The influence of endothal and 6-benzyladenine on crop load and fruit quality of red ‘Delicious’ apple

Summary

Trial work over a two year period examined the aquatic herbicide endothal as a blossom thinner in conjunction with 6-benzyladenine (CyLex?) as a post-bloom thinner. The aim was to develop an effective thinning programme using endothal and CyLex?, to examine any interactions between the two chemicals, and to assess the impact of endothal on fruit quality. Time of application of endothal was shown to be critical in achieving a satisfactory level of thinning. Multiple applications of lower chemical rates were also successful, and may have potential to provide an effective programme for cultivars with long flowering periods, which are traditionally most difficult to thin. Addition of a surfactant to the spray mix significantly increased the thinning effect of endothal, resulting in severe over-thinning. This work has demonstrated that endothal is an effective thinner of red ‘Delicious’ apples. It is most effective when applied twice during the blossom period, the first application at 20% bloom and the second at 80% bloom. There is no advantage in increasing the concentration above 1.0 cm³ dm^-3 (v/v). Endothal can also be effectively combined in a programme with the post-bloom thinner CyLex? applied at 150 mg l”¹ at a spray volume of 12001 ha^-1. Addition of CyLex? to the spray programme has the added benefits of increasing fruit weight and size. Fruit sugar content and firmness were both increased by application of endothal. The greater the number of applications the higher the firmness and sugar content of the fruit. Both these findings are additional benefits to the thinning effect. Both pip number and fruit length/diameter ratio were slightly reduced by endothal. Cylex and some endothal treatments increased the incidence of fruit russet.     

Efficacy of Natureseal® AS1 browning inhibitor in fresh-cut fruit salads applications,with emphasis on apple wedges

Summary

Prevention of browning in fresh-cut fruit is of major commercial significance as fresh-cut fruit salads are major players in the international marketplace. Natureseal^® AS1 and AS5 browning inhibitors out-performed ascorbic acid and citric acid in tests with fresh-cut wedges of ‘Bramley’ apple. A dipping regime of 2 min in 6% (w/v) AS1 solution was found to be optimal, as longer dip-times and higher concentrations led to significant residues of AS1 on the fresh-cut ‘Bramley’ wedges. Tests on fresh-cut wedges from ten apple cultivars indicated that AS1 was significantly beneficial (P < 0.001) to maintain wedge colour during chill-storage in all cultivars, with the exception of ‘Shampion’, which had a low browning tendency and performed equally well with or without AS1. Tests on the anti-oxidant status of a generic fruit salad, which was not treated with AS1 and stored for 15 d, indicated a significant reduction in anti-oxidant status, due largely to the depletion of vitamin C (from 137 to 97 mg 100 g^?1 DW). Total phenolics contents remained relatively constant over the 15-d storage period. The outcomes from these trials have been presented to commercial food companies with a view to their uptake.     

The effect of various storage temperatures on peroxidase activity and protein PAGE gel electrophoresis in ‘Queen’ pineapple fruit

Mature ‘Queen’ pineapple fruit were stored for 30 days at various temperatures and afterwards examined for internal quality. A storage temperature of 8°C is at present generally accepted as the optimum. It was found that peroxidase (PO) activity is correlated with storage temperature and the appearance of storage injury symptoms. High storage temperatures of 10°C, 12°C and 16°C resulted in a high PO activity with internal browning of the core and pulp whereas temperatures of 2°C, 4°C and 6°C resulted in a low PO activity and white watery pulp. To verify these results, polyacrylamide gel electrophoresis was carried out with the proteins in the fruit. The difference in the two extreme temperatures of 2°C and 16°C resulted in a high intensity of high molecular mass proteins at 2°C and an increase in intensity of low molecular mass proteins at 16°C. There is a distinct “change over” of these protein band intensities at 8°C to 12°C. It is presumed that the correct storage temperature will be within these two limits.