Diversity, distribution and management of yam landraces (Dioscorea spp.) in Southern Ethiopia

Yam (Dioscorea spp.) is widely grown in many parts of Ethiopia and plays a vital role in local subsistence. Nevertheless, its diversity has not been studied in detail. A survey covering 339 farm households and eight districts was conducted in the major yam growing regions of Southern Ethiopia to investigate the diversity and distribution of yam landraces using structured and semi-structured questionnaires. A total of 37 named landraces were recorded, with a range from one to six (mean 2.9) on individual farms. Farmers’ decisions regarding the number and type of landraces maintained was influenced by tolerance of the landraces to drought, their maturity time and market demand. Most landraces had limited abundance and distribution, and only a few dominant landraces were widely grown. There was also variation amongst districts with respect to diversity, distribution and abundance of the landraces found. In the majority of the localities surveyed, farmers reported a decreasing trend in the number of landraces maintained on individual farms and in the overall yam production. Besides, in those limited areas where yam production is expanding, farmers are increasingly relying on a few selected landraces that mature early. Findings of this study suggest that local farmers in Wolayita and Gamo-Gofa zones maintain considerable yam diversity that remains to be further explored for sustainable utilization and conservation of the available genetic resources.     

The enset (<Emphasis Type="Italic">Ensete ventricosum</Emphasis>) gardens of Sidama: composition,structure and dynamics of a traditional poly-variety system

The composition and structure of 300 enset gardens from 10 different locations in Sidama were examined. Average size of the gardens was 0.74 ha. The gardens are composed of mixtures of various named enset varieties and different groups recognisable on the basis of ‘sex’ (male and female) and use (cooking and processing). The gardens exhibit high diversity, with a given garden containing up to 24 varieties (mean = 8.2). However, the components are not represented equally. Typically, the gardens consisted of one or two very abundant varieties and a much larger number of less common ones. Enset gardens are dynamic because the number and composition of varieties and different types changed from garden to garden, from location to location and across time. Their composition, structure and dynamics have been influenced by human, socio-economic factors (farm size, land per capita, cropping patterns and size of livestock), temporal circumstances and landscape elements (elevation) in which they are found. The nature, characteristics, organization and functions of Sidama enset gardens are outlined and the importance of maintaining mixtures of varieties for farmers is discussed.     

Genetic erosion of Ethiopian tetraploid wheat landraces in Eastern Shewa,Central Ethiopia

Ethiopia is a centre of diversity and hosts rich genetic resources of tetraploid wheats. Through time, the wheat materials were subject to genetic erosion. Closer investigation was made to assess the status of loss, and identify the possible causes by studying two districts from East Shewa. Information from primary and secondary sources was reviewed and analysed. Farmers identified 26 tetraploid wheat landraces (21 from Akaki and 17 from Ejere), which were once widely grown in the area. Of these, only six were currently available. Compared to the formerly available number of landraces, the loss of diversity in the study area was estimated to be 77%. In the Ejere locality, the loss was 100% before the launching of the on farm landrace conservation programme, and for Akaki it was 95%. Major factors that contributed to the loss include: (1) introduction and expansion of bread wheat varieties; (2) expansion of tef; (3) lack of a mechanism to re-supply seeds of tetraploid wheat landraces; (4) decline in size of landholdings; (5) changes in land use and cropping patterns; (6) lack of policy support; and (7) expansion of improved tetraploid wheat varieties. The lessons from this study underscore the importance of strengthening the local seed supply system as a prerequisite for sustaining on farm conservation of landraces. Moreover, it is necessary to initiate diversity studies focusing on the distribution and status of tetraploid wheat landraces across the country. Complementing these by molecular analyses is essential in order to assess the genetic distinctness of the landraces.     

Species Diversity in Wheat Landrace Populations from two Regions of Ethiopia

Wheat (Triticum spp.) landrace populations in Ethiopia are mostly species mixtures. However, no quantitative data is available with regard to their species components. We studied here 32 wheat landrace populations originating from two regions (Bale and Wello). A total of 2559 individual plants, 45–110 plants representing each population, were classified into their species components. Five tetraploid (2n = 4x = 28) and one hexaploid (2n = 6x = 42) wheat species were found in mixtures of varying proportions. These included the tetraploids Triticum durum Desf., Triticum turgidum L., Triticum aethiopicum Jakubz., Triticum polonicum L., Triticum dicoccon Schrank and the hexaploid Triticum aestivum L. Also found, however in a rare frequency, in two populations from Wollo was T. durum Desf. convar. durocompactoides Flaksb. (Triticum pyramidale Percival), which is a very dense spiked durum. Discriminant analysis using seven qualitative traits revealed 91.5% correct classification of the wheat species, beak awn and awn length with the most significant importance. Single species were found in eight of the populations; six were for T. durum and two for T. aethiopicum. Two to three species-combinations were the most frequent; a maximum of four species was recorded in one population. The highest diversity index (H′) observed was 0.44. T. durum was the most predominant species. The hexaploid T. aestivum was found in nine of the Wollo populations and, in one population, its frequency reached up to 35.5%. On altitudinal basis, no clear trend of clinal variation was observed both from the frequency distributions and H′ estimates. The results confirmed that Ethiopian wheats, despite the morphological overlaps, could be classified into their species components with high degree of certainty. For the future, therefore, genetic diversity estimations should be dissolved into their species components for more expeditious utilization and conservation of this important genetic resource.     

Multivariate Analysis of Diversity of Tetraploid Wheat Germplasm from Ethiopia

The variability of 2420 plants of tetraploid (2n = 4x = 28, AABB genome) wheat germplasm representing 121 accessions from Ethiopia were evaluated for 23 agro-morphological traits at Debre Zeit Agricultural Research Center area, Akaki substation, during the 2003 main season using multivariate methods. This enabled to assess the extent and pattern of variation of the germplasm with respect to regions, altitude classes and species and to identify the major traits contributing to the diversity. The first three and two principal components explained about 83 and 80% of the total variance among regions and altitudinal class, respectively. In the first principal component plant height, days to heading, the length and density of the spike, and kernel color were the most important traits contributing to variation that explained about 50% of regional variance. Cluster analysis grouped the accessions into 15 clusters, with 9 accessions remaining ungrouped. Based on the observed patterns of variation, it is concluded that there exists great variation in the germplasm, which provides opportunities to be utilized for genetic improvement.     

Changes of<Emphasis Type="Italic"> Sorghum bicolor</Emphasis> landrace diversity and farmers’ selection criteria over space and time,Ethiopia

Temporal and spatial changes of sorghum landrace diversity and related factors in five farming communities in north Shewa and south Welo, between 1992/1993 and 2000/2001, were studied in order to ascertain the stability of a range of factors that support the maintenance of the crop genetic diversity. The same farmers were interviewed and the same sorghum fields were surveyed both times. Over the 8-year period: the total area planted to sorghum decreased drastically in all five farming communities and a large percentage of the farmers in the communities (Hayk, 69%; Borkena, 68%; Epheson, 51%; Merewa Adere, 72%; and Bati, 54%) decreased the field size planted to sorghum, because of population growth, land redistribution policy, seasonal changes, and stagger cropping followed by interspecies crop displacement; landrace richness increased significantly in Merewa and Borkena, but decreased significantly in Bati, Epheson and Hayk; and farmers’ selection criteria, the reasons for growing specific landraces, increased significantly (10 in 1992/1993 vs. 22 in 2000/2001). Significant differences in field size distribution occur among the five farming communities. In 2000/2001, 22 “generalist” landraces (grown widely across three or more communities), and 46 “specialist” landraces (niche specific, restricted to certain microhabitats in one or two communities) were found. Landrace gains and losses from the farms surveyed in individual communities over the period are demonstrated.     

Microsatellite Markers Revealed the Genetic Diversity of an Old Japanese Rice Landrace ‘Echizen’

Landraces of rice (Oryza sativa L.) are valuable sources of genetic variation that have been lost in advanced cultivars. Seeds of a rice landrace stored for almost 100 years were found on Sado Island in Niigata prefecture, Japan. This report aims to present basic data on the genetic variation of this landrace, which was known as ‘Echizen’. Five samples of ‘Echizen’, consisting of two old samples, one sample maintained on farm, and two lines regenerated from old seeds were compared with other advanced cultivars and landraces using 19 microsatellite markers. Among the five samples of Echizen, the two stored samples showed greater diversity than the other samples. Cluster analysis based on the UPGMA method also showed that old Echizen was a diverse landrace that could cover the genetic diversity of most Japanese rice cultivars.     

Diversity in reproductive characteristics of potato landraces and cultivars for producing true seed

Potato production from true seed, or true potato seed (TPS) is not a new system for this vegetatively propagated crop. Andean farmers have grown potatoes in the cool highlands from true seed for many centuries. TPS appears today as a promising technology in the warm tropics, where diseases impede the production of pathogen-free potato propagules. However, the identification of suitable genetic resources for potato seed production requires the selection of clones that lack antagonism between tuber growth and seed embryo development. Furthermore, the potential TPS parents need to possess characteristics of a sexually propagated crop that is grown for the harvest of its vegetative part, i.e., the tuber. This new process of domestication requires a change in the selection methods, which starts by assessing the potential of available potato clones for both reproductive characteristics and agronomic traits, particularly those of the tuber. Tetraploid landraces and cultivars of the pathogen-tested collection of the Centro Internacional de la Papa were assessed for reproductive and agronomic characteristics during three consecutive years in San Ramón, a Peruvian location in the warm lowland tropics. The clones showed significant variation for all characteristics recorded. The Shannon–Weaver diversity index (H) was highest for flowering duration (0.617) and intensity (0.600). The lowest H was calculated for bee attraction in this location. The overall H was not significantly affected by the year-to-year variation, but some of the characteristics recorded (e.g. flowers per inflorescence, anther type, style length, plus fruit and berry set) showed distinct H according to the year of assessment. Some of the cultivars with promising TPS traits, as determined by their reproductive characteristics, also had outstanding tuber set, weight and yield. Furthermore, hybrid offspring from the selected clones from the pathogen-tested elite sample, did show high tuber yield with appropriate tuber set and reproductive traits as shown by progeny testing. Hence, the approach followed for identifying parental sources as discussed in this article appears to be appropriate.     

Geographic variation in tetraploid wheat (Triticum turgidum spp. turgidum convar. durum) landraces from two provinces in Ethiopia

There is considerable wealth of genetical and morphological variation in tetraploid wheat collected from Ethiopia by past expeditions. Several authors have speculated on the reasons for this concentration of diversity so far removed from the centre of origin of wheats in West Asia. The present study reports results of evaluation carried out on material collected in the early 1970's from Ethiopia. The landraces from the two provinces Shewa and Tigray were found to be distinctly different. This divergence was attributed to the differences in environmental conditions between them. Wide differentiation among landraces within each province was also present. The Ethiopian farmer's practice of growing composite cultivars in the field has resulted in bulk samples of Ethiopian landraces containing several agrotypes. The proportion of total variance due to differences among agrotypes within landraces was by far the greatest found in this study, followed in most cases by the variation among landrace populations within provinces which exceeded, in turn, the variation between province gene pools. The optimal strategy for further germplasm collections and implications for improvement and conservation in locally-based community gene banks are discussed.     

Morphological and farmers cognitive diversity of barley (Hordeum vulgare L. [Poaceae]) at Bale and North Shewa of Ethiopia

Twenty two accessions of barley landrace/farmers' varieties collected from Bale and North Shewa in situ conservation zones were characterised using 18 qualitative and quantitative morphological traits. Phenotypic frequencies for individual qualitative characters across in situ conservation zones, districts, and strategic sites (localities) have shown mixed and some peculiar patterns. Varieties from Bale conservation zone are predominantly white-yellow seeded whereas varieties from North Shewa are purple-black seeded. White-yellow seeded varieties are more frequent in the lower altitude examined, and the purple-black seeded varieties are more frequent at the highest altitude ( 2650 ). While six row types occur at higher frequencies in almost all the sites in North Shewa, the irregular types are dominant at Bale. The highest frequency of six row types is found at an altitude 2650 , whereas the two rowed and irregular types are found below 2650 . Two row types occur at low frequency at both sites. At Bale, glume awn shorter than glume is the most frequent character whereas glume awn longer than glume is more frequent at North Shewa. Glume awn longer than glume appears to increase with increasing altitude. Caryopsis cover is relatively monomorphic with respect to distribution across zones. The covered types are most frequent in almost all altitudinal ranges. Estimates of diversity index (H) for individual qualitative characters suggest that polymorphism is common in varying degrees for most characters, which indicates the existence of wide range of variation. On the basis of cross validation using discriminant function among the quantitative characters, the landrace varieties from North Shewa seem to be more diverse than the ones from Bale. From the results of multiple regression analysis, the zonal variation is significantly associated with all the quantitative morpho-agronomic characters except plant height. The great majority of the varieties from Bale were perfectly identified and named by farmers. The reliability analysis confirmed that there was a remarkable positive degree of consistency between farmers naming of landrace varieties.     

Genetic Diversity in AA and CC Genome Oryza Species in Southern South Asia

The CC genome of Oryza is found in nine species of Oryza that are distributed on all continents having a tropical climate. Three diploid Oryza species with CC genome are found in Africa and Asia to Papua New Guinea. In southern South Asia these three CC genome diploid species can be found, O. eichingeri and O. rhizomatis in Sri Lanka and O. officinalis in India. AA genome wild relatives of rice are also found in the same geographic region. Germplasm of both diploid CC and AA genome Oryza germplasm has recently been collected from Sri Lanka. AFLP analysis was used to compare the genetic diversity of the two Oryza genomes from a similar geographic region in southern South Asia. In addition, the diploid CC Oryza germplasm was also analyzed by RAPD and SSR methodologies and the combined results were analyzed. The results show that in southern South Asia the diploid CC genome species have a high level of genetic diversity compared to the diploid AA genome species. Molecular marker analysis revealed that populations of O. rhizomatis from northern and southeastern Sri Lanka are genetically differentiated. One accession of O. rhizomatis was aligned with O. eichingeri. This accession was collected from the site of O. rhizomatis that is the closest to a population of O. eichingeri. O. eichingeri showed lower genetic diversity than the other two diploid Oryza CC genome species. O. officinalis accessions from Assam, India, and China were genetically less diverged from O. eichingeri and O. rhizomatis than two accessions of O. officinalis from Kerala state, India. The first two authors contributed equally to this research     

Inverse Sequence-tagged Repeat (ISTR) Analysis of Genetic Variability in Forest Coffee (Coffea arabica L.) from Ethiopia

Genetic diversity in forest coffee (Coffea arabica L.) was estimated using inverse sequence-tagged repeat (ISTR) markers. One hundred ninety two samples representing 16 populations of C. arabica from four regions of Ethiopia were analyzed with 12 pairs of forward and backward ISTR primer combinations. A total of 144 reproducible bands were generated out of which 37 (25%) were polymorphic and scored as present (1) or absent (0) data matrix. This data was used to compute Jaccard coefficient to estimate genetic variability among all possible pairs of samples. The proportion of polymorphic bands within populations ranged from 19% for Bale-3, to 54% for Walega-2 populations. Un-weighted pair group method with arithmetic mean (UPGMA) based cluster analysis generated two clusters at 56% similarity value. The samples were clustered on the basis of their geographical origin, which could be attributed to a few region specific banding patterns detected. However, within regions most of the samples failed to cluster on the basis of their respective populations, which may be due to the presence of substantial gene flow between local populations in the form of seedlings carried out by farmers. The results may provide information to develop strategies for in situ conservation.     

Variation of leaf esterases in some Ethiopian tetraploid wheat landraces

Summary Esterase isozymes of tetraploid wheat landraces originating from four localities in the central highlands of Ethiopia were studied using the horizontal starch gel electrophoresis. The landraces studied consisted of 60 Triticum turgidum L., one T. dicoccon Schrank, one T. polonicum L. and an improved local cultivar. One introduced durum cultivar was also included for comparison.A total of 12 esterase zymograms were detected in the landraces. Among these, the types C, A and G were found to be the most prevalent. The esterase isozymes exhibited high variation in the landraces. The esterase zymograms of the introduced durum cultivar and T. dicoccon differed from those of the landraces while the patterns attained in T. polonicum and the improved local cultivar were identical to those of the other landraces.The total number of bands detected was 10, of which 2 were rare, being displayed by only 1 pattern each. The band content of zymograms was in the range of 3–6 and the average was 4. The unique bands were observed in zymograms with both high and low prevalences in the landraces. Two zymograms, including zymogram C which had the highest frequency, occurred in all the localities. The high yielding landraces displayed 4 of the zymograms, one of which belonged to two of the zymograms with widest distribution.     

Quantitave evaluation of Ethiopian landraces of lentil (Lens culinaris)

Summary One hundred and fifty-six landrace populations of lentil (Lens culinaris Medikus) collected from 10 provinces in Ethiopia were evaluated for a set of six quantitative traits at three sites contrasting in altitude. Consistent regional differences among landraces were found for time to flower and maturity, 100-seed weight, number of seeds/pod and plant height. The regional differences were clarified by a discriminant analysis based on 100-seed weight, time to flower and plant height. The lentil of the West Highlands was early and short, that of the North Highlands was large-seeded, whereas lentils from the Central Highlands were the least distinctive group. Selection for seed size was the result of local human preferences. Humans were probably also responsible for the lack of adaptive value of plant phenology in relation to altitude. Selection for seed yield at the low and middle elevation sites gave a positive response to selection at both sites. However, selection for yield at the highland site did not give a positive response elsewhere, indicating that adaptation to highland conditions differed from that at lower elevations.     

Diversity in landraces of summer squash from Jordan

Summary Forty one half-sib families of summer squash (Cucurbita pepo L.) landraces collected from Jordan were evaluated for 17 morphological and horticultural traits at the Agricultural Experimental Station in the Jordan Valley. A large diversity both within and among families was observed for many traits including plant growth, flowering and sex expression, colour of photosynthetic organs, fruit shape and fruit number per plant. Correlation coefficients were significant among many useful traits. The possible utilization of germplasm collections is discussed.     

Rice Diversity Collection,Conservation and Management in Northeastern India

Rice is the principal crop of northeastern region of India, where a considerable range of diversity exists. The germplasm collection has also unfold the occurrence of large number of rice landraces in the region. Between 1985 and 2002, a total 2639 accessions of rice germplasms including their wild relatives have been collected. Most of these germplasm material have been conserved in the National Gene Bank, New Delhi following their necessary characterization and evaluation. With the establishment of the Regional Gene Bank Module with medium-term storage facilities (6–7°C with 40–45% relative humidity) at Barapani, Meghalaya, the rice germplasm conservation (ex situ) have been initiated in the region.     

Diversity distribution and collection of genetic resources of cultivated and weedy type in <Emphasis Type="Italic">Perilla frutescens</Emphasis> (L.) Britton var. <Emphasis Type="Italic">frutescens</Emphasis> and their uses in Indian Himalaya

Perilla frutescens (L.) Britton is a traditional crop of East Asia and sporadically cultivated in different parts of the Himalayas. The Western and the Eastern Himalayan regions of India exhibit rich variability in oilseed Perilla, Perilla frutescens (L.) Britton var. frutescens. The present communication deals with the distribution pattern of diversity and survey, exploration and germplasm collection and its uses in India. Brief information on botanical characterization on the basis of systematic study of cultivated and weedy types and thrust on areas for germplasm collection from diverse habitats have been discussed.     

Genetic Diversity and Phylogenic Relations Among Sahelian Sorghum Accessions

To establish the genetic relationship among Sahelian sorghum [Sorghum bicolor (L.) Moench S.L.] landraces from Burkina Faso were submitted to electrophoretic analysis for 10 enzymatic systems and 18 loci. Four enzymatic systems (ADH, LAP, MDH, PGD) and five loci revealed polymorphism both within and among landraces. Thirty-eight per cent of the landraces were monomorphic in all the 18 loci. The genotypic frequencies in most of the landraces deviated markedly from Hardy–Weinberg proportions due to a major heterozygote deficit, the landrace being homozygous or a mixture of homozygotes. Multivariate analysis yielded three main groups, containing native landraces and five minors, containing introduced cultivars, randomly distributed over the territory. The pattern of allelic occurrence was random and unrelated to external selection pressures. The major diversity among landraces appears to be from genetic shift caused by farmers’ selection of their seeds. It could also be due to the low rates of outcrossing (19%) and migration (0.06) prevailing in the set.     

Genetic Diversity and Relationships of Wheat Landraces from Oman Investigated with SSR Markers

Little is known about genetic diversity and geographic origin of wheat landraces from Oman, an ancient area of wheat cultivation. The objectives of this study were to investigate the genetic relationships and levels of diversity of six wheat landraces collected in Oman with a set of 30 evenly distributed SSR markers. The total gene diversity, (H_T), conserved in the three durum wheat (Triticum durum desf.) landraces (H_T = 0.46) was higher than in the three bread wheat (Triticum aestivum L.) landraces (H_T = 0.37), which were similar to Turkish and Mexican bread wheat landraces calculated in previous studies. Genetic variation partitioning (G_ST) showed that variation was mainly distributed within rather than among the durum (G_ST = 0.30) and bread wheat (G_ST = 0.19) landraces. Based on modified Rogers’ distance (MRD), the durum and bread wheat landraces were distinct from each other except for a few individuals according to principal coordinate analysis (PCoA). One bread wheat landrace (Greda) was separated into two distinct sub-populations. A joint cluster analysis with other landraces of worldwide origin revealed that Omani bread wheat landraces were different from other landraces. However, two landraces from Pakistan were grouped somewhat closer to Omani landraces indicating a possible, previously unknown relationship. Implications of these results for future wheat landrace collection, evaluation and conservation are discussed.