The effect of some carbon substrates on denitrification rates and carbon utilization in soil

Summary Soil was amended with a variety of carbon sources, including four soluble compounds (glucose, sucrose, glycerol and mannitol) and two plant residues (straw and alfalfa).. Potential denitrification rates, measured both as N₂O accumulation and NO₃ ^– disappearance, were compared, and the predicted values of available C, measured as CO₂ production and water-extractable C, were assessed.The two measures of denitrification agreed well although N₂O accumulation was, found to be most sensitive. Soil treated with the four soluble C compounds resulted in the same rate of denitrification although glycerol was not as rapidly oxidized. Alfalfa-amended soil produced a significantly higher rate of denitrification than the same amount of added straw. CO₂ evolution was found to be a good predictor of denitrification over the first 2 days of sampling, but neither measure of available substrate C correlated well with denitrification rate beyond 4 days, when NO₃ ^– was depleted in most treatments. The data with alfalfa-amended soil suggested that denitrifiers used water-extractable C. materials produced by other organisms under anaerobic conditions.     

Elevated CO2 stimulates N2O emissions in permanent grassland

To evaluate climate forcing under increasing atmospheric CO₂ concentrations, feedback effects on greenhouse gases such as nitrous oxide (N₂O) with a high global warming potential should be taken into account. This requires long-term N₂O flux measurements because responses to elevated CO₂ may vary throughout annual courses. Here, we present an almost 9 year long continuous N₂O flux data set from a free air carbon dioxide enrichment (FACE) study on an old, N-limited temperate grassland. Prior to the FACE start, N₂O emissions were not different between plots that were later under ambient (A) and elevated (E) CO₂ treatments, respectively. However, over the entire experimental period (May 1998–December 2006), N₂O emissions more than doubled under elevated CO₂ (0.90 vs. 2.07 kg N₂O-N ha⁻¹ y⁻¹ under A and E, respectively). The strongest stimulation occurred during vegetative growth periods in the summer when soil mineral N concentrations were low. This was surprising because based on literature we had expected the highest stimulation of N₂O emissions due to elevated CO₂ when mineral N concentrations were above background values (e.g. shortly after N application in spring). N₂O emissions under elevated CO₂ were moderately stimulated during late autumn–winter, including freeze–thaw cycles which occurred in the 8th winter of the experiment. Averaged over the entire experiment, the additional N₂O emissions caused by elevated CO₂ equaled 4738 kg CO₂-equivalents ha⁻¹, corresponding to more than half a ton (546 kg) of CO₂ ha⁻¹ which has to be sequestered annually to balance the CO₂-induced N₂O emissions. Without a concomitant increase in C sequestration under rising atmospheric CO₂ concentrations, temperate grasslands may be converted into greenhouse gas sources by a positive feedback on N₂O emissions. Our results underline the need to include continuous N₂O flux measurements in ecosystem-scale CO₂ enrichment experiments.     

Effect of addition of various carbon substrates on denitrification in a vertic Mollisol

Summary Glucose, acetate, malate, and citrate were added to an agricultural soil. The pe values (-log e^-; calculated from the redox potential) obtained 30 min after the addition of C were not correlated with the theoretical reducing power nor with the theoretical total energy of the C compounds. By contrast the number of electron (e^-) equivalents was correlated with pe⁷, indicating that the proton number affected the redox potential (Eh) measurement. After 24 h of incubation, denitrification rates followed the order citrate>malate>glucose and control. No N₂O production was detected with acetate. Denitrification was not correlated with the theoretical reducing power of the added C compounds but was correlated with pe+pH. Similar numbers of e^- equivalents were measured with all treatments. After 72 h of incubation, the order of the denitrification rates was malate>citrate >acetate>glucose and control. The Eh values (lower than after 24h) did not differ with treatment while the number of e^- equivalents was influenced by the quality of the C source. This also demonstrates that the proton number affected the measured Eh. Our results suggest that the different C substrates did not directly influence the soil physicochemical and biological conditions through their degree of oxidation. Any effects appeared to be indirect, arising from the ability of the substrates to generate new metabolites, and consequently initiate different metabolic pathways that modified the soil physicochemical conditions, reducing power and microbial activity.     

Denitrification in intact subsoil cores

 Intact, unamended subsoil cores taken from two contrasting field sites were incubated in the laboratory at 12  °C under aerobic (21% O₂) and anaerobic (1.1–5.5% O₂) conditions. Denitrification of ¹⁵N-NO₃ ^– diffusing into the cores across a semi-permeable membrane was estimated by: (1) direct observation of ¹⁵N-labelled N₂ and N₂O, and (2) mass balance following sectioning at the end of the experiment. The in situ denitrification potential (rates where the supply of NO₃ ^– is not limited by diffusion) was estimated using a finite-difference approximation to a diffusion reaction equation based on comparison of time and depth profiles of NO₃ ^– and Br^– in the incubated cores. Potentials between 0.2 and 2.5 mg N kg^–1 day^–1 were obtained under low O₂ and natural C concentrations. These results indicate the potential for denitrification in glacial till subsoils to reduce NO₃ ^– leaching to ground or surface waters to levels unlikely to result in a pollution hazard. The major product of NO₃ ^– reduction in these subsoils was observed to be N₂, rather than the greenhouse gas and catalyst of stratospheric O₃ removal, N₂O. Received: 31 October 1997     

Denitrification,N2-fixation and fermentation during anaerobic incubation of soils amended with glucose and nitrate

Nitrate and glucose additions were investigated for their role in the C and N dynamics during anaerobic incubation of soil. A gas-flow soil core method was used, in which the net production of N₂, N₂O, NO, CO₂, and CH₄ under a He atmosphere could be monitored both accurately and frequently. In all experiments clayey silt loam soil samples were incubated for 9 days at 25 °C. Addition of nitrate (50 mg KNO₃-N kg^-1 soil) had no effect on total denitrification and CO₂ production rates, while the N₂O/N₂ ratio was affected considerably. The cumulative N₂O production exceeded the cumulative N₂ production for 6 days in the treatment with nitrate addition, compared to 1.2 days in the unamended treatment. Glucose addition stimulated the microbial activity considerably. The denitrification rates were limited by the growth rate of the denitrifying population. During denitrification no significant differences were observed between the treatments with 700 mg glucose-C kg^-1 and 4200 mg glucose-C kg^-1, both in combination with 50 mg KNO₃-N kg^-1. The N₂ production rates were remarkably low, until NO _inf3 ^sup- exhaustion caused rapid reduction of N₂O to N₂ at day 2. During the denitrification period 15–18 mg N kg^-1 was immobilised in the growing biomass. After NO _inf3 ^sup- shortage, a second microbial population, capable of N₂-fixation, became increasingly important. This change was clearly reflected in the CO₂ production rates. Net volatile fatty acid (VFA) production was monitored during the net N₂-fixation period with acetate as the dominant product. N₂-fixation faded out, probably due to N₂ shortage, followed by increased VFA production. In the high C treatment butyrate became the most important VFA, while in the low C treatment acetate and butyrate were produced at equal rates. During denitrification no VFA accumulation occurred; this does not prove, however, that denitrification and fermentation appeared sequentially. The experiments illustrate clearly the interactions of C-availability, microbial population and nitrate availability as influencing factors on denitrification and fermentation.Dedicated to Professor J. C. G. Ottow on the occasion of his 60th birthday     

不同利用方式红壤反硝化势和气态产物排放特征

采用厌氧培养-乙炔抑制法测定了4种不同利用方式红壤的反硝化势和气态产物N₂O和N₂的排放速率。结果表明，不同利用方式红壤反硝化势和N₂O和N₂的排放速率差异明显，土壤反硝化势强弱顺序依次为：竹林>茶园>林地>旱地。反硝化势与土壤有机碳(P<0.05)、厌氧培养期间土壤CO₂累积排放量(P<0.01)、nirS基因丰度( P<0.05)和nirK基因丰度(P<0.05) 呈显著正相关关系。逐步回归分析结果表明，CO₂累积排放量表征的易矿化碳是造成不同利用方式红壤反硝化势差异的主要原因，可以解释反硝化势变化的66%(P<0.01)。不同利用方式红壤N₂O和N₂排放速率差异明显，旱地红壤N₂O和N₂排放速率均最低，表明土壤pH的提升并没有增加旱地红壤的反硝化损失风险和N₂O排放速率。土壤易矿化有机碳含量也是影响不同利用方式红壤N₂O和N₂排放速率的主要因素。反硝化功能基因nirS、nirK和nosZ的丰度均与CO₂累积排放量呈显著正相关关系，进一步支持了土壤易矿化有机碳含量是影响不同利用方式红壤反硝化势和气态产物排放的主要因子。土壤pH是影响不同利用方式红壤反硝化气态产物N₂/N₂O的主要因素，但是pH影响红壤N₂/N₂O的微生物机制仍需要进一步研究。     

Saccharidic compounds as soil redox effectors and their influence on potential N2O production

Cellulose, xylan, and glucose were compared in waterlogged soil as modifying factors of the redox potential (Eh), of the quantity of reducing equivalents, and of the soil capacity to produce N₂O and CO₂. During the study period (168 h) soils supplied with glucose and xylan showed a higher Eh decrease than the control soil and the soil treated with cellulose. In samples taken after 0, 24, 48, and 168 h, the soils supplied with C showed a higher number of reducing equivalents than the control soil did. These quantities were not correlated with Eh values, nor with N₂O production. N₂O production was increased compared with the control soil over the entire experimental period in the glucose-amended soils but only after 48 h in the xylan-amended soils and not until 168 h in the cellulose-treated soils. The CO₂:N₂O ratio was consistently higher than the theoretical value of 2, suggesting that denitrification and CO₂ production via fermentation occurred simultaneously. Moreover, this ratio was highly correlated with the Eh values. We conclude that more research is needed to explain the role of soil redox intensity (Eh) and capacity (quantity of redox species undergoing reduction) in the expression of soil denitrification-fermentation pathways.     

稻田反硝化速率测定方法研究进展

反硝化作用是淹水稻田肥料氮损失的主要途径之一.采用合适的反硝化测定方法是开展稻田反硝化作用研究的前提.然而,由于反硝化过程主要产物N2的大气背景值较高,以及反硝化作用具有高度时空异质性,淹水稻田反硝化作用损失氮量难以准确量化一直是阻碍科学评价稻田气态氮损失的关键难题.本文综述了研究稻田反硝化作用的4种方法(乙炔抑制法、...     

Effects of C and N availability and soil-water potential interactions on N2O evolution and PLFA composition

Mitigation of agricultural N₂O emissions via management requires quantitative information about the regulation of the underlying processes. In this laboratory study, short-term evolution of N₂O from repacked soil was determined using an arable sandy loam soil adjusted to three water potentials (−15, −30 or −100 hPa) that were reached by adjustment of partly air-dried soil with nutrient solutions or water; a water retention curve of repacked soil had been determined prior to the incubation experiment. The amendments included a control treatment receiving water (CTL), and aqueous solutions of carbon in the form of glucose (C), ammonium sulfate (N), or both (CN). Rates of CO₂ and N₂O evolution were followed during 14 days. Soil inorganic N and phospholipid fatty acid (PLFA) composition were analyzed by the end of incubation. Across all nutrient treatments, the soil at the lower moisture content (−100 hPa) showed little or no N₂O evolution irrespective of nutrient treatment. Adding glucose alone reduced N₂O evolution relative to CTL. The addition of N alone had no effect on soil respiration, but significantly increased nitrate accumulation and N₂O evolution. The CN treatment resulted in higher respiration than with C amendment alone, but less N₂O evolution than with N alone, at least at −15 and −30 hPa. Whole-soil PLFA fingerprints at the end of incubation reflected the complex response of gaseous emissions. At −15 hPa growth of Gram negative bacteria, probably including denitrifiers, in the CN treatment was indicated by low cyclopropane-to-precursor ratios. At −100 hPa differentiation of branched-chain fatty acids was taken as evidence for an effect of C amendment on Gram positive bacteria. The highest potential for N₂O evolution was observed at the intermediate soil wetness level; the corresponding gas diffusivities indicated that this parameter may be a better predictor of N₂O emissions than water-filled pore space.     

Soil N Losses by Denitrification Evaluated Using the 15N Tracer Method

Molecular nitrogen (N₂) and nitrous oxide (N₂O) generated by denitrification increase N losses in the soil–plant system. This study aimed to quantify N₂ and N₂O from potassium nitrate (K¹⁵NO₃) applied to soils with different textures and moisture contents in the absence and presence of a source of carbon (C) using the ¹⁵N tracer method. In the three soils used (sandy texture (ST), sandy clay loam texture (SCLT), and clayey texture (CT)), three moisture contents were evaluated (40%, 60%, and 80% of the water holding capacity (WHC)) with (D⁺) and without (D^?) dextrose added. The treatments received 100 mg N kg^?1 (KNO₃ with 23.24 atom% ¹⁵N). N₂ emissions occurred in all of the treatments, but N₂O emissions only occurred in the D⁺ treatment, showing increases with increasing moisture content. SCLT with 80% WHC in the D⁺ treatment exhibited the highest accumulated N emission (48.26 mg kg^?1). The ¹⁵N balance suggested trapping of the gases in the soil.     

Nitrous oxide emissions from agricultural soils at low temperatures: a laboratory microcosm study

We studied in laboratory microcosms (intact soil cores) N₂O and CO₂ emissions from four different agricultural soil types (organic soil, clay, silt and loam) at low temperatures with or without freezing-thawing events. When the temperature of the frozen soil cores was increased stepwise from −8 °C the N₂O emissions began to increase at −0.5 °C, and peaked at −0.1 °C in the organic, clay and silt soils, and at +1.6 °C in the loam soils. However, a stepwise decrease in soil temperature from +15 °C also induced an increase in the N₂O emissions close to the 0 °C. These emissions peaked between −0.4 and +2.5 °C depending on the soil type and water content. However, the emission maxima were from 2 to 14.3% of those encountered in the experiments where frozen soils were thawed. Our results show that in addition to the well-documented thawing peak, soils also can have a maximum in their N₂O emission near 0 °C when soil temperature decrease. These emissions, however, are less than those emitted from thawing soils. The correlations between the N₂O and CO₂ emissions were weak. Our results suggest that N₂O is produced in soils down to a temperature of −6 °C.     

Microbial processes and the site of N2O production in a temperate grassland soil

To understand nitrous oxide (N₂O) emissions from terrestrial ecosystems it is necessary to understand the processes leading to N₂O production. Here, for the first time, results are presented which identify in situ the processes of N₂O production in a temperate grassland soil. A small portion of the nitrogen (N) applied in the summer to the grassland soil was rapidly transported below the main rooting zone (>20 cm) and resulted in large N₂O productions at depths of 20-50 cm. Preferential pathways must have been responsible for this movement because the soil conditions were not conducive to leaching by piston flow. The N₂O was entirely produced by nitrate (NO₃⁻) reduction which was surprising because the bulk soil was aerobic. Therefore, reduction processes can operate during times of the year when it is least expected and cause large N₂O concentrations deep in the soil profile.     

Changes in chemical and biological properties of a sodic clay subsoil with addition of organic amendments

An experiment was performed to examine the chemical and biological effects on high clay sodic subsoil following the incorporation and incubation with organic amendments. The main treatments consisted of amendments with wheat shoots, lucerne pellets and peat, and these were compared to gypsum addition. Additional treatments were residues of chickpea and canola, chicken manure and sawdust. All materials were finely ground and added to crushed and sieved soil at the rate of 1% by weight. Wheat, canola and chickpea residues and chicken manure resulted in modest reductions in soil sodicity. Carbon and N mineralization were related to the soluble C/total N ratio in the amendment. The initial mineralization of wheat amendment was rapid due to its soluble C content, but then slowed to have the lowest loss, of around one third of added C, of all the plant residues after 174 days. In comparison, lucerne-amended soil increased total N and lost almost half of its C after the 174-day incubation. The canola stubble amendment showed the highest carbon loss, losing 64% of its added C. The addition of gypsum resulted in high soil electrical conductivity which suppressed respiration, compared to the control soil, indicating a detrimental effect on microbial activity due to the high electrolyte concentration in the soil. The peat amendment, with a low-soluble C content, showed a similar respiration rate to the control soil, confirming that a source of soluble C is important for the initiation of rapid biological activity. Soil pH was significantly increased (by 0.6 of a pH unit) with addition of chicken manure, and still remained higher than control soil after 174 days of incubation. Lucerne was the only plant residue to increase soil pH, with the effect being sustained for 56 days. The study demonstrated how some organic amendments can improve chemical fertility and biological activity in high clay sodic subsoil, and at the same time contribute, after 25 weeks incubation, to an increase in carbon content.     

典型菜地土壤剖面N2O、CH4与CO2分布特征研究

为探究菜地土壤剖面N2O、CH4与CO2时空分布特征,利用地下气体原位采集系统与气相色谱法,周年动态监测3种典型菜地,即休闲裸地、轮作地Ⅰ(芹菜?空心菜?小白菜?苋菜)以及轮作地Ⅱ(菜心?芹菜?空心菜?大青菜)7 cm、15 cm、30 cm与50 cm土层N2O、CH4与CO2浓度变化。结果表明,0~50 cm土层范围内,N2O、CH4与CO2 3种气体浓度周年变异性较大,变幅分别为0.63~1 657.0μL(N2O)?L?1、0.8~72.5μL(CH4)?L?1和0.41~36.6 m L(CO2)?L?1。轮作地Ⅰ与轮作地Ⅱ的N2O平均浓度随土壤深度增加而增加,休闲裸地则呈现先增加(0~30 cm)后降低(30~50 cm)的变化趋势。两种轮作菜地4个土层N2O平均浓度均显著高于休闲裸地,二者氮肥施用量不同并未造成相同土层间N2O平均浓度的显著差异。3种菜地CH4与CO2平均浓度均呈现50 cm30 cm15 cm7 cm的梯度特征。轮作地Ⅰ与轮作地Ⅱ0~15 cm土层CH4平均浓度均大于休闲裸地,而在15~50 cm土层则分别大于和小于休闲裸地。CO2浓度呈现明显的季节性变化,除轮作地Ⅰ50 cm土层外,两种轮作菜地其他土层CO2平均浓度均小于休闲裸地对应土层。可见,蔬菜地高氮肥施用、多频次耕作等复杂管理使得N2O、CH4与CO2表现出较大的时空变异特征,其中氮肥施用对N2O的影响大于CH4与CO2,CH4受施肥与耕作的影响均较小,CO2显著受土壤温度与耕作措施的影响,在此基础上需进一步探究N2O、CH4与CO2的其他影响因素。     

Effect of dairy manure type and supplemental synthetic fertilizer on methane and nitrous oxide emissions from a grassland in Nasu,Japan

Abstract

It is well known that some fungal species are remarkably tolerant of high copper concentration, although copper is toxic to most fungi (Garraway and Evans 1984). Bedford (1936) and Jurkowska (1952) reported that Penicillium and Aspergillus species can grow in liquid media saturated or nearly saturated with copper sulfate. Okamoto and Fuwa (1974) isolated Penicillium ochro-chloron from the laboratory air, and found that the fungus was able to grow in a medium saturated with copper sulfate.     

Fate of nitrogen and carbon in the vadose zone: in situ and laboratory measurements of seasonal variations in aerobic respiratory and denitrifying activities

In situ and laboratory measurements of aerobic respiratory and denitrifying activities were studied in the vadose zone (almost 2.5 m thick) of a fluvic hypercalcaric cambisol characterized by transitory anaerobic conditions. A field experiment was conducted in a bare soil, over a 7-month period starting just after maize harvest and incorporation of maize crop residues. Weather variables (air and soil temperature, rainfall), soil water content, soil solutes (NO₃⁻ and dissolved organic carbon) and soil gases (CO₂ and N₂O), were recorded throughout the experiment. Four soil layers were defined. Bacterial counts were performed in each layer using the most probable number (MPN) method. Aerobic respiratory and denitrifying activities were estimated from laboratory measurements. In situ microbial activity, as revealed by CO₂ and N₂O measurements in the soil atmosphere, was strongly influenced by weather. Laboratory measurements showed that potential aerobic respiratory activity (ARA) occurred throughout the soil profile, whereas semi-potential denitrifying activities SPDA (i.e. measured under organic-C limiting condition) occurred mainly in the top 30 cm soil layer. In the soil profile, the CO₂ concentration gradient was stronger than the N₂O concentration gradient. Seasonal variations in microbial activities increased with depth, whereas DOC concentrations, and variations in those concentrations, decreased with depth, suggesting that DOC quality investigations are necessary in the deep vadose zone to understand microbial activities seasonal variations. Laboratory measurements of potential activities agreed well with in situ microbial activity in natural environmental conditions. NO₃⁻ was a stronger limiting factor for SPDA than was denitrifier density in the soil profile.     

CO2, CH4 and N2O fluxes of upland black spruce (Picea mariana) forest soils after forest fires of different intensity in interior Alaska

Abstract

Forest fires can change the greenhouse gase (GHG) flux of borea forest soils. We measured carbon dioxide (CO₂), methane (CH₄) and nitrous oxide (N₂O) fluxes with different burn histories in black spruce (Picea mariana) stands in interior Alaska. The control forest (CF) burned in 1920; partially burned (PB) in 1999; and severely burned (SB1 and SB2) in 2004. The thickness of the organic layer was 22 ± 6 cm at CF, 28 ± 10 cm at PB, 12 ± 6 cm at SB1 and 4 ± 2 cm at SB2. The mean soil temperature during CO₂ flux measurement was 8.9 ± 3.1, 6.4 ± 2.1, 5.9 ± 3.4 and 5.0 ± 2.4°C at SB2, SB1, PB and CF, respectively, and differed significantly among the sites (P < 0.01). The mean CO₂ flux was highest at PB (128 ± 85 mg CO₂-C m^?2 h^?1) and lowest at SB1 (47 ± 19 mg CO₂-C m^?2 h^?1) (P < 0.01), and within each site it was positively correlated with soil temperature (P < 0.01). The CO₂ flux at SB2 was lower than that at CF when the soil temperature was high. We attributed the low CO₂ flux at SB1 and SB2 to low root respiration and organic matter decomposition rates due to the 2004 fire. The CH₄ uptake rate was highest at SB1 [–91 ± 21 μg CH₄-C m^?2 h^?1] (P < 0.01) and positively correlated with soil temperature (P < 0.01) but not soil moisture. The CH₄ uptake rate increased with increasing soil temperature because methanotroph activity increased. The N₂O flux was highest [3.6 ± 4.7 μg N₂O-N m^?2 h^?1] at PB (P < 0.01). Our findings suggest that the soil temperature and moisture are important factors of GHG dynamics in forest soils with different fire history.     

Denitrification in grass swards is increased under elevated atmospheric CO2

Emissions of N₂O and N₂ were measured from Lolium perenne L. swards under ambient (36 Pa) and elevated (60 Pa) atmospheric CO₂ at the Swiss free air carbon dioxide enrichment experiment following application of 11.2 g N m⁻² as ¹⁵NH₄¹⁵NO₃ or ¹⁴NH₄¹⁵NO₃ (1 at.% excess ¹⁵N). Total denitrification (N₂O+N₂) was increased under elevated pCO₂ with emissions of 6.2 and 19.5 mg ¹⁵N m⁻² measured over 22 d from ambient and elevated pCO₂ swards, respectively, supporting the hypothesis that increased belowground C allocation under elevated pCO₂ provides the energy for denitrification. Nitrification was the predominant N₂O producing process under ambient pCO₂ whereas denitrification was predominant under elevated pCO₂. The N₂-to-N₂O ratio was often higher under elevated pCO₂ suggesting that previous estimates of gaseous N losses based only on N₂O emissions have greatly underestimated the loss of N by denitrification.     

Soil Moisture Controls the Denitrification Loss of Urea Nitrogen from Silty Clay Soil

Relative control of soil moisture [30, 60, and 80 percent water-holding capacity (WHC)] on nitrous oxide (N₂O) emissions from Fargo-Ryan soil, treated with urea at 0, 150, and 250 kg N ha^?1 with and without nitrapyrin [2-chloro-(6-trichloromethyl) pyridine] (NP), was measured under laboratory condition for 140 days. Soil N₂O emissions significantly increased with increasing nitrogen (N) rates and WHC levels. Urea applied at 250 kg N ha^?1 produced the greatest cumulative N₂O emissions and averaged 560, 3919, and 15894 µg kg^?1 at 30, 60, and 80 percent WHC, respectively. At WHC ≤ 60 percent, addition of NP to urea significantly reduced N₂O losses by 2.6- to 4.8-fold. Additions of NP to urea reduced N₂O emission at rates similar to the control (0 N) until 48 days for 30 percent WHC and 35 days for 60 and 80 percent WHC. These results can help devise urea-N fertilizer management strategies in reducing N₂O emissions from silty-clay soils.     

东鱼河春季沉积物反硝化脱氮作用与N2O排放研究

为研究东鱼河春季沉积物的反硝化脱氮作用以及N_2O产生速率,于2017年5月沿东鱼河采集沉积物和表层水样,分别采用乙炔抑制法和培养法分析测定了反硝化作用和N_2O产生速率,利用顶空法提取水样中N_2O结合双层扩散模型法估算水体N_2O的排放通量,并结合水体理化性质探讨了主要影响因素。结果表明:东鱼河春季沉积物反硝化潜势为7 305.8～26 947.7μmol/(m~2·h),但从上游到下游沿程均呈先增高后波动降低的趋势;沉积物反硝化速率为86.6～694.2μmol/(m~2·h),显著低于对应点位的反硝化潜势(P0.01),且二者的沿程变化规律不一致;反硝化速率仅与沉积物中NH_4~+含量呈显著正相关关系(P0.05),表明其受沉积物和上覆水理化性质的综合影响,且可能存在硝化—反硝化的耦合作用。沉积物N_2O产生速率在19.8～144.3μmol/(m~2·h);水体表现为大气N_2O的排放源,排放通量为170.9～667.8mol/(m~2·h),显著高于对应点位沉积物N_2O的产生速率(P0.01),且与上覆水的DO和NO_2~-含量具有显著正相关关系(P0.05),表明东鱼河在春季除沉积物产生N_2O外,其上覆水中氮转化过程亦是向大气排放N_2O的重要途径之一。