生物絮凝养殖条件下吉富罗非鱼的消化能力和非特异性免疫能力研究

为研究生物絮凝养殖条件下吉富罗非鱼的消化能力和免疫能力,采用室内循环水养殖系统(对照组)和生物絮凝养殖系统(实验组)饲养吉富罗非鱼(24.17±2.49)g 59 d。结果显示,与对照组相比,实验组水体中总氨氮(TAN)和亚硝态氮(NO-2-N)浓度均显著高于对照组,分别为(20.48±14.66)和(33.77±34.57)mg/L。但实验结束时实验组终末密度(FD)高达36.89 kg/m3,终末质量(FIW)、增重率(WGR)和特定生长率(SGR)均高于对照组(P0.05),饵料系数(FCR)比对照组降低18.7%(P0.05)。实验组饲料氮利用率为57.97%,显著高于对照组(49.51%)。实验组和对照组中罗非鱼的胃蛋白酶、胃脂肪酶、肠蛋白酶和肠脂肪酶活性无显著差异。除肝胰脏总超氧化物歧化酶(T-SOD)活性显著低于对照组外,实验组血清、肝胰脏和头肾的T-SOD、溶菌酶(LZM)、碱性磷酸酶活力(AKP)活性较对照组无差异(P0.05)。     

Effects of biochar on microbial community in bioflocs and gut of Oreochromis niloticus reared in a biofloc system

Aquaculture International - This study investigated the effects of biochar application on the bacterial community structure in bioflocs and intestines of Oreochromis niloticus cultured in a biofloc...     

Crude protein levels in diets for two growth stages of Nile tilapia (Oreochromis niloticus) in a biofloc system

Biofloc technology (BFT) provides an additional feed source for aquatic organisms through the conversion of waste in microbial flocs. Because of this, the suitable protein level in a diet for animals in this system could be different from those in conventional systems. Our objective was to determine the suitable protein level in the diet of Nile tilapia juveniles reared with BFT. Two experiments were carried out with tilapia weighing ~10 g (first experiment) and about 50 g (second experiment) during 61 and 98 days respectively. Five crude protein (CP) levels (within 17% and 33%) were tested. The increment of CP resulted in a reduction in dissolved oxygen, pH, alkalinity and an increase in dissolved phosphorus, total suspended solids and total ammonium nitrogen. The crude protein level had a significant effect (p < 0.05) on animal performance. The linear response plateau model was the best fit for the final weight and weight gain data in the two growth phases. In conclusion, tilapia juveniles of 10–60 g and 60–230 g in biofloc can be fed on diets with 28% of CP (26% of digestible protein) and 22% CP (20% of digestible protein) respectively.     

Comparative analysis of nitrogen and phosphorus budgets in a bioflocs aquaculture system and recirculation aquaculture system during over-wintering of tilapia (GIFT,Oreochromis niloticus)

Nitrogen (N) and phosphorus (P) budgets in a bioflocs technology (BFT) aquaculture system and a recirculation aquaculture system (RAS) during over-wintering of tilapia (GIFT Oreochromis niloticus)for 64 d were compared in the current study. Fish feed was the major input of N in both systems, specifically, 94±0 % and 82±4 % for the RAS and BFT aquaculture system, respectively. The rate of N recovery in the BFT aquaculture systems was estimated to be 48±5 % of input N, which was significantly different from that of the RAS (37±4 %). There was no significant difference between the RASs and BFT aquaculture systems in terms of P recovery rate. The regular backwashing of the drum filter and biological filter in RAS accounted for 41 ± 2 % of input N and 39 ± 2 % of input P. Approximately 54 % of unassimilated nitrogen N was removed by nitrification in the BFT aquaculture systems. The results from the present study suggest that nitrification may be the dominant pathway for ammonia removal in a BFT aquaculture system rather than by heterotrophic bacterial assimilation.     

Changes in amino acids and essential fatty acids during early larval rearing of dentex

Samples of fertilized dentex, Dentex dentex, eggs and larvae fed enriched rotifers and Artemia according to standard hatchery procedures were analysed for free, total amino acid and fatty acid contents. Egg free amino acids (53 nmol ind-1) and total lipids (13.5 (g ind-1) levels were considerably reduced in the newly hatched larvae (6.0 nmol and 5.7 (g ind-1 respectively) while the amount of -3 PUFA (Polyunsaturated fatty acid) and 20:4-6 remained unchanged suggesting their possible role as essential fatty acids (EFA) in this species. In general, the changes in pattern of the major biochemical components during early development of dentex was similar to that outlined for other Sparidae species investigated to date. The essential amino acid profile of enriched live food did not show major imbalances when compared to that of dentex larvae. In contrast, the level of docosahexaenoic acid (22:63, DHA) and the DHA:EPA (eicosapentaenoic acid, 20:5-3) ratio in larvae given rotifers and Artemia were significantly lower relative to the corresponding values in the unfed larvae.     

Apparent digestibility coefficient of protein and amino acids of acid, biological and enzymatic silage for Nile tilapia (Oreochromis niloticus)

Using the fish silage to partially replace proteic feedstuff in aquafeeds is an alternative to mitigate sanitary and environmental problems caused by the lack of adequate destination for fisheries residues. It would also lower feed costs, consequently improving fish culture profitability. However, using fish silages in aquafeeds depends on determination of its apparent digestibility coefficients (ADC). This work aimed to determining the ADC of crude protein and amino acids of acid silage (AS), biological silage (BS) and enzymatic silage (ES) for juvenile Nile tilapia (94.5 ± 12.7 g). The ADC_CP was: 92.0%, 89.1% and 93.7% for AS, BS and SE respectively. The average ADC of amino acids was: 91.8%, 90.8% and 94.6% for AS, BS and ES respectively. Results encourage the use of AS, BS and ES to partially replace protein sources in balanced diets for neotropical fish.     

The use of biochar in the production of tilapia (Oreochromis niloticus) in a biofloc technology system - BFT

The present study investigated the effectiveness of biochar as a water quality control agent and an alternative carbon source in a tilapia-based biofloc technology (BFT) system. Water quality parameters were measured following standard procedures (APHA, 1998) and fish physiological indices were measured using designated ELISA kits. Data was analyzed using repeated measures ANOVA and one-way ANOVA. Two biochar treatments were employed i.e. B; only biochar, GB; biochar + glucose, while for the control (G) only glucose was added. Tilapia fingerlings were cultured for approximately 10 weeks with no water exchange. Significant reduction (p < 0.05) in dissolved oxygen (DO) level was observed in group G (5.99 ± 0.10 mg/L) compared to B (6.66 ± 0.12 mg/L) and GB (6.53 ± 0.56 mg/L). Notably, concentrations of total suspended solids (TSS) were signficantly lower in the biochar treatments (B and GB) compared to the control (G). Additionally, alkalinity was significantly higher in G (156.47 ± 5.17 mg/L) compared to B (137.92 ± 3.83 mg/L) and GB (146.44 ± 3.87 mg/L). Levels of NO₃- and total nitrogen (TN) were significantly higher in group B compared to G and GB. Also, significant difference was observed in PO₄^3- levels between B (30.81 ± 2.28 mg/L) and GB (22.70 ± 1.84 mg/L). The dynamics of the water quality parameters revealed that heterotrophic assimilation and nitrification were active in maintaining the water quality. Overall, the result showed no significant differences (p > 0.05) in all the fish growth parameters. Urea nitrogen measured in the fish was significantly lower in B (3.85 ± 0.32 mmol/L) compared to G (5.40 ± 0.39 mmol/L) and GB (5.53 ± 0.39 mmol/L). Generally, there were no notable detrimental effects of biochar application on fish growth and physiological performance.     

Changes in lipid classes,fatty acids,protein and amino acids during egg development and yolk‐sac larvae stage in brill (Scophthalmus rhombus L.)

Brill (Scophthalmus rhombus L.) is a flatfish considered of special interest for aquaculture diversification, but the high mortality observed during the early larval rearing is the main obstacle to commercial culture. The objective of this study was to contribute to the knowledge of nutrient utilization of early‐hatched larvae, characterizing the changes in lipid and protein contents during embryogenesis and the yolk‐sac larval stage of S. rhombus. Total lipid, lipid classes and fatty acid contents remained constant during embryogenesis and yolk‐sac larval development, except for phosphatidylethanolamine and phosphatidylinositol, which increased in quantity during the yolk‐sac larval stage. On the other hand, total protein (including non‐protein nitrogen) and amino acids decreased their contents in both periods, especially at hatching. The decrease only in the serine, glutamic acid, proline and lysine contents during embryogenesis suggests a selective use of amino acids during this phase. Unlike embryogenesis, amino acids loss during hatching appears to be non‐selective, and almost all amino acids (essential and non‐essential) decreased. Our results suggest that there is higher catabolism of protein vs. lipid during embryogenesis and the yolk‐sac larval stage of S. rhombus.     

Compensatory growth of Nile tilapia Oreochromis niloticus,L. subjected to cyclic periods of feed restriction and feeding in a biofloc system

The compensatory growth, productive performance, proximal composition and somatic indices of Nile tilapia (Oreochromis niloticus) cultivated in biofloc were evaluated during a 144‐day period under five cyclic regimes of feed restriction and feeding. Five treatment groups, in which the frequency of feed restriction (R) and feeding (F) varied by periods (days) as follows: R1:F3, R3:F9, R6:F18, R8:F24 and R12:F36; each treatment was evaluated in triplicate. The cycles were repeated throughout the culture period. The control group received feed daily. Fish were cultivated in 18 circular tanks (3 m³) at a density of 50 fish/m³ per tank. At the end of the study, the survival of Nile tilapia was greater than 90% in all the treatments. Complete compensation in growth was achieved in R6:F18 and R12:F36. At the end of the feed restriction period, both crude protein and total lipid content of the tilapia muscle tissue taken from fish of the treatment groups were similar to samples of muscle tissue derived from fish of the control group; however, a reduction of more than 40% in somatic indices compared with the control was observed, but these recovered by the end of the feeding phase. The results indicate that cyclic feeding based on 12 days of feed restriction and 36 days of feeding (R12:F36) induced a complete compensation in weight and restoration of energy reserves, with similar measures of productive performance observed when compared to the control treatment during the culture of Nile tilapia in biofloc, and food reduction did not affect proximal composition.     

Moderate salinities enhance growth performance of Nile tilapia (Oreochromis niloticus) fingerlings in the biofloc system

The biofloc technology (BFT) has recently gained attention as an economic and environmentally sustainable system for aquaculture. The use of BFT with salinized water could be used to minimize the negative effects of nitrogenous waste and improve the growth performance of Nile tilapia. This work evaluated the growth performance, survival, gill lesions, and fillet composition of tilapia fingerlings (Oreochromis niloticus) reared during 70 days with different salinities (0, 4, 8, 12, and 16 g/L) in a biofloc previously developed. The results indicated that the use of mature bioflocs alone was sufficient to avoid fingerling mortality due to nitrite peaks. Moreover, the salinized water, especially between 4 and 8 g/L (maximum points around 6 g/L), can be recommended in BFT to improve the growth performance of tilapia in the initial culture phase. The salinity level that was evaluated did not affect fillet composition nor the occurrence of gill lesions. The total ammonia nitrogen concentration was lower in freshwater than in salinized water (p < 0.05). Nitrite peaks in salinities of 12 and 16 g/L appeared later and were higher in comparison to other treatments. Considering minimal water exchange in BFT, the use of salinized water in this system may be environmentally viable.     

几种牡蛎在保鲜中游离氨基酸和牛磺酸的变化

牡蛎是一种营养丰富的水产动物,曾被誉为“海洋牛奶”［１］。其高营养价值和医疗保健作用,在很大程度上取决于含有较高的风味营养物质。这些物质在细胞内起着调节渗透压的作用,同时也赋予牡蛎所特有的鲜味和营养。据报道［２］,海产动物体内游离氨基酸、牛磺酸等的变化与原有的含量水平、细胞的自溶作用和随之而发生的微生物生长繁殖有紧密关系。８０年代初,日本学者研究鲐、鲣、金枪鱼等在冷藏过程中,从新鲜状态到初期腐败阶段ＦＡＡ含量变化与相关鲜度指标的关系［３．４］ 我国牡蛎主要品种有僧帽牡蛎（Ｏｓｔｒｅａ ｐｌｉａｔｕ…