Correction to: Seroprevalence of bovine brucellosis and associated risk factors in Nakasongola district,Uganda

Tropical Animal Health and Production - In the originally published article, the name of the fifth author was incorrectly presented as Godfroid Jacques. The correct name is Jacques Godfroid.     

Seroprevalence and associated risk factors of bovine brucellosis in Addis Ababa dairy farms

A cross-sectional study was carried out on bovine brucellosis in Addis Ababa dairy farms from November 2003 to April 2004. A total of 1,202 blood samples were collected from non-vaccinated, cross-bred dairy cattle. The Rose Bengal plate test (RBPT) was used as a screening test. Those serum samples reacting positively to RBPT were subjected to the complement fixation test (CFT) for confirmation. The RBPT detected 30 of 1,202 (2.5%) of the samples as brucellosis positive. The positive sera when further retested using CFT, 18 out of the 30 RBPT positive sera were confirmed to be positive. The prevalence of brucellosis based on CFT in the study area was 1.5%, and all positive sera were from female cattle. Result of the questionnaire survey revealed that percentage of 4.4% abortion and 9.5% retained fetal membranes. Abortion and retained fetal membranes were associated with Brucella antibodies (P?相似文献   

Seroprevalence and risk factors for bovine brucellosis in Jordan

We investigated the seroprevalence and risk factors for Brucella seropositivity in cattle in Jordan. The sera from 671 cows were randomly collected from 62 herds. The antibodies against Brucella were detected using a Rose Bengal plate test and indirect ELISA. A structured questionnaire was used to collect information on the cattle herds'' health and management. A multiple logistic regression model was constructed to identify the risk factors for Brucella seropositivity. The true prevalence of antibodies against Brucella in individual cows and cattle herds was 6.5% and 23%, respectively. The seroprevalence of brucellosis in cows older than 4 years of age was significantly higher than that in the younger cows. The seroprevalence of brucellosis in cows located in the Mafraq, Zarqa and Ma''an governorates was significantly higher than that of the other studied governorates. The multiple logistic regression model revealed that a larger herd size (odd ratio = 1.3; 95% CI: 1.1, 2.6) and mixed farming (OR = 2.0; 95% CI: 1.7, 3.7) were risk factors for cattle seropositivity to Brucella antigens. On the other hand, the use of disinfectants (OR = 1.9; 95% CI: 1.1, 2.1) and the presence of adequate veterinary services (OR = 1.6; 95% CI: 1.2, 3.2) were identified as protective factors.     

Seroprevalence and potential risk of bovine brucellosis in zerograzing and pastoral dairy systems in Uganda

A cross-sectional study was conducted in Uganda between November 2006 and February 2007 to assess the seroprevalence and risk of brucellosis in zerograzing and pastoral dairy systems; two major sources of milk in Uganda, 80% of which is sold unpasteurized to consumers through informal channels. A total of 723 cattle comprised of 497 animals from the pastoral system and 226 animals from the zerograzing system were tested for antibodies against natural B. abortus infection using the competitive enzyme-linked immunosorbent assay (C-ELISA). Herd-level seroprevalence was 100% in the pastoral system and 5.5% (95% CI: 1.8, 9.2) in the zerograzing system. The animal-level seroprevalence and within-herd range of brucellosis in cattle in the pastoral system were 34.0% (95% CI: 29.9, 38.1) and 8.1–75.9%, while for those in the zerograzing system were 3.3% (95% CI: 0.9, 5.7) and 0–9.0%. Abortion rates of 23% and 0% among seropositive cows vis-à-vis 5.4% and 1.9% among seronegative cows were recorded in the pastoral and zerograzing systems, respectively. The risk of natural B. abortus infection was higher among older cattle (>24 m) (Odds ratio [OR] = 1.83, 95% CI: 1.25–2.67) and dry cows (OR = 2.01, 95% CI: 1.23–3.31) in the pastoral system, and in calves aged 0–6 m (OR = 5.72, 95% CI: 1.04–31.41) in the zerograzing system. Implementing a culling program in the zerograzing system to eliminate the existing low risk of brucellosis and targeting calves in the pastoral systems for vaccination could avert the cost-related limitation of brucellosis control in Uganda.     

Seroprevalence of brucellosis and its associated risk factors in cattle from smallholder dairy farms in Zimbabwe

A cross-sectional study was conducted to investigate seroprevalence of brucellosis and the associated risk factors in cattle from smallholder dairy farms in Gokwe, Marirangwe, Mushagashe, Nharira, Rusitu and Wedza areas of Zimbabwe. A total of 1,440 cattle from 203 herds were tested serially for Brucella antibodies using Rose Bengal test and the competitive ELISA. Weighted seroprevalence estimates were calculated and risk factors in individual cattle investigated using logistic regression analysis. The overall individual animal brucellosis seroprevalence was low, with mean of 5.6% (95% confidence interval (CI), 4.4%, 6.8%). Gokwe had the highest individual (12.6%; 95% CI, 3.9%, 21.4%) and herd-level (40.0%; 95% CI, 22.1%, 58.0%), while Wedza had the lowest individual (2.3%; 95% CI, 0%, 5.3%) and herd-level (8.0%; 95% CI, 0.0%, 18.9%) brucellosis seroprevalence, respectively. In individual cattle, the area of origin, age and history of abortion were independently associated with brucellosis seroprevalence. While the seroprevalence was independent of sex, it decreased with increasing age. Cattle 2–4 years old had higher odds (odds ratio (OR) = 3.2; 95% CI, 1.1%, 9.1%) of being seropositive compared to those >7 years. Cows with a history of abortion were more likely to be seropositive (OR = 7.9; 95% CI, 3.1, 20.1) than controls. In conclusion, the area-to-area variation of brucellosis may be linked to ecological factors and differences in management practices. The implementation of stamping out policy, bleeding and testing animals before movement and promoting the use self-contained units are likely to significantly reduce the public health risks associated with Brucella infections in cattle.     

Seroprevalence investigation of bovine brucellosis in Macenta and Yomou,Guinea

Brucellosis is one of the world’s major zoonotic diseases associated with reproductive disorders and a potential infection of human. Brucellosis leads to serious economic losses due to late-term abortion, stillbirth, weak calves, and sterility. In Guinea, the data on brucellosis was only detected as far back as 10 years ago. The purpose of this study was to estimate the prevalence of bovine brucellosis in the provinces of Macenta and Yomou of Guinea. A structured questionnaire was used in the clinical study, and 345 cattle were clinically examined. Three hundred serum samples were initially subjected to the Rose Bengal test (RBT); the positive results of which were confirmed by the complement fixation test (CFT). The investigation indicated that farmers had little information on brucellosis. Hygroma, abortion, sterility, and placental retention were the observed symptoms. Of the 29 RBT-positive samples, 26 were confirmed by CFT. The prevalence of brucellosis in Macenta and Yomou was 12 and 5.33 %, respectively. In both provinces, the prevalence mean was 8.67 %. This study highlighted the immediate necessity to carry out a strengthened surveillance of human and animal brucellosis to obtain as many data as possible in order to establish strategies for prevention and management of brucellosis in Guinea.     

Seroprevalence and risk factors associated with bovine viral diarrhea virus and bovine herpes virus-1 in Zanjan Province,Iran

Tropical Animal Health and Production - Bovine viral diarrhea virus (BVDV) and bovine herpes virus-1 (BHV-1) remain as the major pathogens with heavy economic consequences in Iran. The prevalence...     

Seroprevalence of and risk factors for brucellosis of goats in herds of Michoacan, Mexico

Our objectives were to estimate the seroprevalence of Brucella melitensis, and to identify some risk factors associated with goat seropositivity in Michoacan, Mexico. Blood samples were collected from 5114 animals from 79 herds. Sera were tested for antibodies against B. melitensis using the Rose Bengal plate test and the complement-fixation test. Information regarding the herds and each animal sampled were recorded through a personal interview at the farm. We used random-effects multivariable logistic regression to analyze our data. Fifty-six herds of the 79 tested had at least one seropositive animal. The animal-level true seroprevalence was 9.8% (CI = 8.8, 10.7). Animals in large herds (>34 animals), in herds with high stock density (>3.5 animals/m²) or animals >24 months old had higher odds of seropositivity (2.0, 1.7 and 1.8, respectively) than those in small herds, in herds with low stock density or animals ≤24 months old.     

Prevalence and associated risk factors of mycobacterial infections in slaughter pigs from Mubende district in Uganda

To date, the public health relevance of mycobacterial infections in pigs is not well investigated despite high risk of infection. Recently, there has been a documented increase in opportunistic infections and risk of acquiring opportunistic mycobacterial infections in HIV/AIDS patients in Mubende district; unfortunately, there has been no published information on the epidemiology of mycobacterial infections in this area. This study was carried out between September 2008 and February 2009. Investigations were done to assess the prevalence and associated risk factors of mycobacterial infections in slaughtered pigs in Mubende district of Uganda. A total of 997 pigs (53.7% male and 46.3% female) from 31 different slaughterhouses were examined for the presence of lesions compatible with TB and mycobacterial infections. Pathologic tissue specimens were collected for culturing and isolation of mycobacteria. A cross-sectional technique was used based on convenient visits to slaughterhouses but random selection of individual slaughtered pigs for a detailed post-mortem inspection on a daily basis. The results reflected a 9.3% and 3.1% (95% CI) prevalence of Mycobacterium species based on necropsy examinations and culture isolation, respectively. The highest prevalence of mycobacterial infection was recorded in Buwekula County (the mixed agro-zone) whilst the lowest was in Kassanda County (pastoral zone). A multivariable logistical regression analysis identified age (P ≤ 0.001) and sex (P ≤ 0.05) as risk factors for mycobacterial infections in pigs. Post-estimation statistics of the regression model evaluation and validation fit it well into the data (HL, χ ² = 5.9; P = 0.69 for necropsy, HL χ ² = 2.9; P = 0.94 for culturing). This study documented a high prevalence of mycobacterial infections in slaughter pigs in Mubende district. The fact that pigs and human often share common housing and environment poses a high risk of zoonotic transmission. This then warrants further molecular investigation to identify the specific Mycobacterium species and their public health importance in this area.     

Prevalence and risk factors associated with bovine brucellosis in herds under extensive production system in southwestern Nigeria

Using a cross-sectional survey, we determined the prevalence and risk factors associated with bovine brucellosis in herds under extensive production system in southwestern Nigeria. Antibodies to Brucella species in serum samples were tested using the Rose Bengal test (RBT) and competitive enzyme-linked immunosorbent assay (cELISA); for milk, the milk ring test (MRT) and indirect-ELISA (i-ELISA) were used. Questionnaire was administered to cattle herdsmen to determine factors predisposing the animals to bovine brucellosis. Data were analyzed using STATA 12. From 513 serum and 635 milk samples tested among 120 herds, overall animal-level prevalence of 10.1% (95% CI 7.5–12.7%) and 20.2% (95% CI 17.1–23.3%) were recorded by RBT and MRT, respectively; while 9.4% (95% CI 6.9–11.9%) and 17.8% (95% CI 14.8–20.8%) were obtained using cELISA and i-ELISA, respectively. In all, from the 120 herds tested, 29.2% and 43.3% were positive by RBT and MRT, respectively. Multivariable logistic regression revealed that herd location (OR?=?8.12, 95% CI 1.68–38.90) and improper disposal of placenta/fetus (OR?=?17.33, 95% CI 4.81–62.33) were predictors for a seropositive herd using RBT; while herd location (OR?=?5.13, 95% CI 1.27–20.28), large herd size (OR?=?2.62, 95% CI 1.15–5.85), and occurrence of abortion for a year or more (OR?=?4.62, 95% CI 1.53–13.71) were predictors of seropositivity to antibodies to Brucella spp. using MRT. We found high prevalence of brucellosis in cattle herds under extensive management system in southwestern Nigeria. Urgent and coordinated control strategies are required to mitigate this problem.

     

Responses to tuberculin among Zebu cattle in the transhumance regions of Karamoja and Nakasongola district of Uganda

Responses to tuberculin in Zebu cattle of the transhumant pastoral farming system in Karamoja region and Nakasongola district in the north-eastern and mid-central regions in Uganda, respectively, were investigated using a comparative intradermal tuberculin skin test. Of the 1864 cattle tested from 30 large units (superherds) in Karamoja and 7 herds in Nakasongola, a total of 28 animals from 19 herds (51.4%) tested positive. Inter-district tuberculin reactor prevalence variations seemed to be influenced by climate, with impact on both the management patterns and transmissibility of agent. High herd tuberculin reactor prevalence (51.4%) was attributed to widespread contacts and mixing of animals between herds. Low individual animal tuberculin test positivity (mean = 1.4%) was attributed to low transmissibility of the agent under the Karamoja climate, which is semi-arid, and to increased resistance due to non-specific response to environmental mycobacteria and natural selection, since there was no active control against bovine tuberculosis. Owing to similarities in management practices in Karamoja and widespread risk factors, it was difficult to identify which were more important, but variations in sources of drinking water pointed to provision of lake and borehole water during dry season as reducing the risk. Positive bovine tuberculin reactor prevalence and skin reactor status were related to age.     

Seroprevalence and risk factors associated with Equine piroplasmosis in North Egypt

Equine piroplasmosis (EP) is caused by Theileria equi and/or Babesia caballi and has economic importance particularly in equines reared in poor management systems. This study is based on cELISA test to study the seroprevalence of EP among 370 horses and 150 donkeys in four Governorates north Egypt. Additionally, its risk factors were studied for the first time. The seroprevalence rates 36.5 %, 20 %, and 5.6 % for T. equi, B. caballi, and mixed infections, respectively. The highest antibody levels against T. equi were detected in Kafr ElSheikh (40 %) and Giza (40.1 %) Governorates, whereas those of B. caballi were detected in Qalyubia (25 %) and Kafr ElSheikh (24.1 %) Governorates. Concerning T. equi, animals >10 years (OR = 2.06) were more likely to be infected with EP than those <5 years old. In addition, the seropositivity increased among grazing (OR = 5.7, 95 % CI: 1.73−19.27) males (OR = 1.8, 95 % CI: 1.23−2.61) infested with ticks (OR = 2.3, 95 % CI: 1.60−3.48) during summer (OR = 4.3, 95 %CI: 2.53−7.46); whereas the seropositivity of animals for B. caballi increased among grazing equines (OR = 7.8, 95 % CI: 1.05−58.25) over 10 years old (OR = 2.08, 95 % CI: 1.10−3.94) and infested with ticks (OR = 2.4, 95 % CI: 1.54−3.76) during summer (OR = 7.12, 95 % CI: 3.15–16.06). Therefore, EP is an important prevalent disease in Egypt and deserves further attention regarding the management system, treatment, and vector control.     

Seroprevalence and associated risk factors of important pig viral diseases in Bhutan

A cross-sectional serological study was conducted in Bhutan between October 2011 and February 2012 to determine the prevalence of antibodies to classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), swine influenza virus (SIV) subtype H1N1 and Aujeszky's disease virus (ADV). Furthermore, risk factors for the seropositive status were investigated.     

Seroprevalence of and risk factors for infectious bovine rhinotracheitis in beef cattle herds of Yucatan,Mexico

The main objective of this cross-sectional study was to estimate the seroprevalence of infectious bovine rhinotracheitis (IBR) in a population of non-vaccinated beef cattle in the livestock region of Yucatan, Mexico and to determine potential risk factors related to the seroprevalence. Also, we estimated the intraherd correlation (r_e) and design effect (D) of IBR seropositivity. Cattle were selected by two-stage cluster sampling. Blood samples were collected from 564 animals from 35 herds. Sera were tested for antibodies against IBR using the serum-neutralisation test. Information regarding the herd and each animal sampled were recorded through a personal interview with the farmer or farm manager. The data were analysed using fixed-effects logistic multiple regression. Thirty-four of the 35 herds had at least one seropositive animal. The animal true seroprevalence was 54.4%. Animals in large herds or in production had higher odds of seropositivity than those in small herds or growing. The r_e and D were 0.17 and 3.62, respectively.     

Seroprevalence and associated risk factors of Chlamydia abortus infection in ewes in Tunisia

Enzootic abortion of ewes (EAE) caused by Chlamydia abortus is a disease of ruminants that results in serious economic losses in livestock industry. The zoonotic potential of the pathogen adds a public health concern on the efforts to control the disease. We report herein a cross-sectional study that was conducted during the lambing season (June and July) in Tunisia to estimate the seroprevalence of C. abortus infection in large sheep herds with abortion history. A total of 803 ewes were sampled and tested using indirect enzyme linked immunosorbent assay (iELISA). The overall apparent seroprevalence at herd and individual levels were 58 % (95 %CI = 39–74.5 %) and 6.6 % (95 %CI = 4.9–8.3 %), respectively. Significant risk factors investigated using univariate and multivariate analyses were history of infertility (OR = 5.7; 95 %CI = 3.05–10.66), the number of reproductive ewes (OR = 2.1; 95 %CI = 1.12–3.94), the control of new animals at introduction (OR = 4.35; 95 %CI = 2.46–7.68), the sharing of drinking water (OR = 2.18; 95 %CI = 1.22–3.9), the exchange of breeding males (OR = 2.56; 95 %CI = 1.003–6.54), the disposal of abortion materials without precaution (OR = 4.36; 95 %CI = 2.42–7.87), the lack of lambing barn (OR = 2.39; 95 %CI = 1.13–5.04), the non-application of hygienic post-abortion measures (OR = 10.35; 95 %CI = 5.28–20.26) and the manure management (OR = 11.35; 95 %CI = 3.26–39.48). To the best of our knowledge, this is the first sero-epidemiological survey conducted on an abortive disease in Tunisian ewes that investigated the risk factors of C. abortus infection.     

Sero-prevalence of bovine brucellosis and its risk factors in Jimma zone of Oromia Region,South-western Ethiopia

A cross sectional sero-prevalence study was conducted on 1,595 cattle in Jimma zone, Ethiopia to investigate the status of bovine brucellosis and identify potential risk factors. Sera samples were analyzed using Rose Bengal Plate Test (RBPT) and Complement Fixation Test (CFT). The overall individual and herd level sero-prevalences were 3.1% (n = 1,595) and 15.0% (n = 227), respectively. The sero-prevalence of bovine brucellosis at individual animal level was significantly higher in non-pregnant (11.18%) than pregnant (2.77%) and lactating (22.35%) than non-lactating animals (2.46%). Moreover, significantly higher sero-prevalence was observed in herds of larger sizes. Individual animal sero-prevalence was also positively associated with the occurrence of abortion (26.98 and 1.54% in those with and without previous history of abortion, respectively). Generally, the sero-prevalence of bovine brucellosis found in Jimma area was not high and the sero-prevalence was closely associated with some of the risk factors considered at individual animal and herd level.