The final established physicochemical properties of steamed bread made from frozen dough: Study of the combined effects of gluten polymerization,water content and starch crystallinity on bread firmness

The gluten polymerization behavior, water content, starch crystallinity and firmness of Chinese steamed bread made from frozen dough were investigated and their correlations were also established in this study. The decreased degree of gluten polymerization in steamed bread was observed by the enhanced SDS-extractable proteins (SDSEPs) upon frozen storage. Less incorporation of glutenin in the glutenin–gliadin crosslinking of steamed bread mainly contributed to the decreased degree of gluten polymerization. The decreased moisture of steamed bread had a significant negative correlation with the sublimated water in frozen dough (r = −0.8850, P < 0.01). Frozen storage also induced an increase in starch crystallinity and bread firmness. A multiple linear regression model with SDS-extractable proteins, water content and melting enthalpy of starch crystals of steamed bread accounted for 86% of the variance in the natural logarithm of firmness and further revealed that starch crystallinity mainly contributed to bread firmness.     

Effect of inulin with different degree of polymerization on plain wheat dough rheology and the quality of steamed bread

The aim of this study was to evaluate the effect of different polymerization degree of inulin on plain wheat dough rheology and quality of steamed bread. It was found the water absorption of dough decreased with the increasing of short-chain (FS) and natural inulin (FI) and increased with the increasing of long-chain inulin (FXL) higher than 7.5%. Three kinds of inulin all increased the development time, stability and farinograph quality number and decreased softening degree of the dough. When proof time was less than 90min, the extensibility increased with the substitution of 5% of FS, 5% of FI and 2.5% of FXL. The resistance to extension, ratio number of resistance to extensibility and energy all increased with the increasing of FS and FI as well as the time. While the energy increased with FXL substation at 45min and dropped thereafter, regardless of the concentration. The addition of inulin all enhanced the brightness, specific volume and hardness of steamed bread and decreased the water content, vaporization enthalpy, springiness, recovery, and cohesiveness. During the storage, inulin reduced the change rates of relative hardness, recovery, and cohesiveness and increased the change rate of relative enthalpy, which restrained the staling rate of steamed bread.     

Study of the mechanism of improvement due to waxy wheat flour addition on the quality of frozen dough bread

Waxy wheat flour (WWF) was substituted for 10% regular wheat flour (RWF) in frozen doughs and the physicochemical properties of starch and protein isolated from the frozen doughs stored for different time intervals (0, 1, 2, 4 and 8 weeks) were determined to establish the underlying reasons leading to the effects observed in WWF addition on frozen dough quality. Using Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimeter (DSC) and X-ray Diffraction (XRD) among others, the gluten content, water molecular state, glutenin macropolymer content, damaged starch content, starch swelling power, gelatinization properties, starch crystallinity and bread specific volume were measured. Compared to RWF dough at the same frozen storage condition, 10% WWF addition decreased dry gluten and glutenin macropolymer contents and T₂₃ proton density of frozen dough, but increased the wet gluten content, T₂₁ and T₂₂ proton density. 10% WWF addition also decreased damaged starch content, but increased starch swelling power, gelatinization temperature and enthalpy, crystallinity of starch and bread specific volume of frozen dough. Results in the present study showed that the improvement observed due to WWF addition in frozen dough bread quality might be attributed to its inhibition of redistribution of water molecules bound to proteins, increase in damaged starch content and decrease in starch swelling power.     

中麦175馒头和面条品质稳定性分析

为给优质小麦育种和生产提供参考依据,以优质小麦新品种中麦175于2010-2011年度在河北和北京14个地点的样品为材料,分析了磨粉品质、面粉和面片颜色、面团流变学和淀粉糊化特性、馒头和面条加工品质。结果表明,中麦175为馒头和面条兼用型优质品种,特点是软质、中偏弱的面筋强度、面粉颜色白,多数品质性状较稳定,籽粒硬度、PPO活性、面片a*值、稳定时间、拉伸面积、延展性和最大抗延阻力的变异系数较大,馒头加工品质地点间变异大于面条。磨粉品质和面团流变学参数对馒头加工品质有显著影响,籽粒硬度和出粉率对馒头表面颜色有显著负向影响,相关系数分别为-0.82(P<0.01)和-0.58(P<0.05);面粉L*值高,馒头加工品质好,二者相关系数为0.72(P<0.01);吸水率与馒头总分呈显著负相关(r=-0.84,P<0.01),稳定时间、拉伸面积、延展性和最大抗延阻力与馒头总分呈显著正相关,相关系数分别为0.85(P<0.01)、0.77(P<0.01)、0.62(P<0.05)和0.70(P<0.01)。降低PPO活性和吸水率,提高蛋白质含量、出粉率和黄色素含量可以改善面粉和面片颜色的亮白度,增加部分黄度,形成消费者可接受的奶白色。淀粉糊化特性对馒头和面条加工品质无显著影响。上述信息对改良小麦品质的稳定性有重要意义。     

新疆冬小麦品种品质性状与面包、馒头、面条加工品质的关系

为了给优质面包、馒头、面条专用品种选育提供品质辅助选择指标,以30份新疆冬小麦品种(包括自育品种和引进品种)为材料,分析了新疆冬小麦品种品质性状与面包、馒头、面条加工品质的关系.回归分析结果表明,小麦籽粒性状、面粉品质、面团特性、淀粉糊化特性以及面粉色泽对新疆冬小麦品种面包、馒头和面条加工品质均有显著影响;面粉灰分、湿面筋、稀懈值、亮度和红度是影响面包、馒头和面条加工品质的共同品质性状;形成时间、稳定时间、延展度是影响面包和面条加工品质的共同品质性状;而籽粒性状仅对新疆冬小麦品种馒头加工品质有显著影响.相关分析结果表明,千粒重、形成时间、稳定时间、拉伸面积、最大拉伸阻力、红度和黄度与面包总分呈显著相关关系,相关系数分别为0.460、0.516、0.537、0.719、0.707、0.534和-0.403;籽粒蛋白质含量、面粉蛋白含量和湿面筋含量与馒头总分呈显著相关关系,相关系数分别为-0.397、-0.458和-0.552,面团延展度、稀懈值与面条总分呈显著相关关系,相关系数分别为0.438和0.432.从以上结果可以看出,面包与面团流变学特性,馒头与蛋白质和面筋数量,面条与面团流变学特性及淀粉糊化特性的关系更为密切,这些品质性状可以作为新疆冬小麦品种面包、馒头、面条加工品质改良时的辅助选择指标.     

Soy ingredients stabilize bread dough during frozen storage

Bread with 48.5% soy ingredients was assessed for quality during frozen storage of the dough. Soy protein was hypothesized to prevent water migration during frozen storage, thereby producing dough that would exhibit fewer structural changes than traditional wheat bread. Wheat and soy bread were baked from dough that was fresh or frozen (−20 °C, 2 or 4 wks). Dough and bread were assessed for physical properties including moisture content, percent “freezable” and “unfreezable” water, dough extensibility, and bread texture. The bread was subjected to an untrained sensory panel. The soy bread was denser, chewier, and had a higher moisture content than wheat bread. When baked from fresh or frozen dough, soy bread was rated “moderately acceptable” or higher by 70% of panelists. Soy minimized changes in dough extensibility and resistive force to extension, leading to minimal changes in bread hardness. Although consumers could distinguish between bread baked from soy dough that was fresh or frozen for 4 wks, sensorial and textural data suggested that the rate at which the quality of the soy dough deteriorated was slower than that of wheat dough. In conclusion, the dough of consumer-acceptable soy bread retained quality characteristics during frozen storage slightly better than wheat dough.     

Effect of glutathione on wheat dough properties and bread quality

Reduced glutathione (GSH), released from lysed yeast cells, is well-known for weakening dough structure. However, its influence on bread texture and staling has not yet been completely elucidated. Herein, this study aims at assessing the effects of GSH on dough properties and bread quality, especially bread staling, using Rheofermentometer analysis, texture profile analysis (TPA), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and low-field nuclear magnetic resonance (LF-NMR). The results revealed that GSH substantially decreased the dough height and gas-retention capacity during fermentation. The weakened dough structure was attributed to GSH-induced disulfide bond cleavage in gluten proteins and the depolymerization of glutenin polymers. The addition of 0.005%–0.01% GSH resulted in acceptable bread quality. However, bread supplemented with 0.015%–0.03% of GSH exhibited a coarser and more open crumb microstructure, as well as high level of hardness and low resilience during aging. GSH promoted crumb water loss and drove the water shift from the immobilized to the bound state. The DSC and XRD analyses further confirmed that GSH promoted starch retrogradation and recrystallization. These results suggest that apart from the gluten structure, GSH also altered gluten−starch interactions and water redistribution, ultimately decreasing bread quality and accelerating bread staling.     

Effects of glycerol on water properties and steaming performance of prefermented frozen dough

The amount of ice in both unfrozen steamed bread dough (UFD) and prefermented frozen steamed bread dough (PFD) with and without glycerol was investigated by differential scanning calorimetry (DSC). The quality of unfrozen steamed bread (UFB)/prefermented frozen dough steamed bread (PFB) was also evaluated. Frozen stability and steaming performance of prefermented frozen dough were negatively correlated with ice crystal growth. Glycerol effectively prevented the formation of ice crystals during freezing and frozen storage, maintaining the quality of steamed bread from prefermented frozen dough even over a period of 30 days. The best steamed bread performance was observed with the dough containing 2% of glycerol (flour weight basis) addition. Prefermenting conditions significantly affected the quality of UFB/PFB. The highest quality scores of steamed bread from prefermented frozen dough were obtained from 32 °C and 85% rh for 40 min.     

Relationship of Protein Quantity,Quality and Dough Properties with Chinese Steamed Bread Quality

Using selected Chinese and Australian wheats, flour protein content and composition of high-molecular-weight (HMW) glutenin subunits were studied in relation to northern style Chinese steamed bread quality. Flour protein content had a significant impact on Chinese steamed bread quality. The Chinese wheats were characterised by shorter Farinograph dough development time and stability in comparison with the Australian wheats. Dough stickiness in the Chinese wheat cultivars was a significant factor deteriorating Chinese steamed bread quality. A significant negative correlation was found between Farinograph stability time and steamed bread quality in Australian wheats while a significant and positive correlation existed in Chinese wheats. It would be necessary to increase the dough strength of Chinese wheat cultivars in order to improve their steamed bread making quality.     

旱地小麦新品种小冰麦143品质特性研究

为了了解旱地小麦新品种小冰麦143的品质特性，采用国际(ICC)、国家(GB)标准分析方法，将小冰走143的部分品质性状(蛋白质品质、面团流变学特性、淀粉特性、蛋白质电泳特性)及其食品(面包、面条、馒头)的加工品质与晋走47和长武134进行了对比与分析。结果表明，小冰麦143的籽粒品质优于长武134和晋走47；蛋白质品质、面团漉变学特性与长武134、晋麦47相近；馒头加工品质优于这两个品种；面包加工品质与这两个品种也相近；加工面条韧性差，干物质失落率比长武134和晋麦47高，面条加工品质较差。参照国家有关标准综合评价，小冰走143是一个具有良好食品加工特性的面包、馒头兼用型小麦新品种。     

Phytate negatively influences wheat dough and bread characteristics by interfering with cross-linking of glutenin molecules

The influence of added phytate on dough properties and bread baking quality was studied to determine the role of phytate in the impaired functional properties of whole grain wheat flour for baking bread. Phytate addition to refined flour at a 1% level substantially increased mixograph mixing time, generally increased mixograph water absorption, and reduced the SDS-unextractable protein content of dough before and after fermentation as well as the loaf volume of bread. The added phytate also shifted unextractable glutenins toward a lower molecular weight form and increased the iron-chelating activity of dough. It appears that phytate negatively affects gluten development and loaf volume by chelating iron and/or binding glutenins, and consequently interfering with the oxidative cross-linking of glutenin molecules during dough mixing. Phytate could be at least partially responsible for the weak gluten network and decreased loaf volume of whole wheat flour bread as compared to refined flour bread.     

豆渣馒头加工工艺研究

研究了豆腐渣粉、谷朊粉、酵母添加量对馒头感官品质和质构特性(TPA)的影响,通过单因素和正交试验确定豆渣馒头的最佳工艺条件,并采用环境扫描电镜研究豆渣馒头的微观结构.结果表明:小麦面粉85 g、豆腐渣粉15 g、谷朊粉1g、活性干酵母0.6g、发酵时间120 min时制作的豆渣馒头具有良好的品质和口感.电镜分析显示,豆...     

Effect of thermal packaging temperature on Chinese steamed bread quality during room temperature storage

In order to investigate the influence of different packaging temperature on thermal-vacuum packaged Chinese steamed bread (CSB) quality under room temperature storage, the packaging temperatures of 50 °C, 70 °C and 90 °C were set respectively to study the effect on the packaging vacuum degree (VD) and starch retrogradation properties. The VD and moisture content of thermal-vacuum packaged CSB significantly increased with the increase of the packaging temperature from 50 °C to 90 °C, while the hardness of CSB decreased, and there was no significant difference in moisture migration during room temperature storage. The results of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and small angle X-ray scattering (SAXS) indicated that CSB packaged at different temperatures formed B-type crystals, leading to an enhanced the ordered and dense state of starch structure, and the semi-crystalline growth ring structure disappeared during storage. The influence of different packaging temperatures on VD of CSB could effectively hinder the formation of ordered and dense state of CSB starch, consequently delaying the crystallization of amylopectin and inhibiting the progress of staling. The result of this study is of great significance for improving the quality of starchy foods and guiding the industrial development of Chinese steamed bread.     

小偃22小麦粉馒头加工技术研究

为了提高馒头的加工品质,满足中国馒头加工业的迫切要求,以小偃22小麦粉为材料,研究其馒头加工的加水量、和面时间以及大豆蛋白、小麦胚芽、卵磷脂对馒头品质的影响。结果表明,加水量、和面时间以及大豆蛋白、小麦胚芽、卵磷脂的添加量对馒头回复性、内聚性和咀嚼性影响显著或极显著。随加水量增加,馒头的内聚性和回复性先增后减,咀嚼性呈减小趋势;随和面时间与大豆蛋白添加量的增加,馒头内聚性和回复性均减小,咀嚼性均先减后增;随小麦胚芽添加量的增加,馒头的内聚性与回复性先减后增,咀嚼性先减后增;随卵磷脂添加量的增加,内聚性先增后减,咀嚼性先减后增,回复性减小。优质馒头制作的适宜加水量、和面时间以及大豆蛋白、卵磷脂和小麦胚芽添加量分别为粉质吸水率的80％、4min、2%、0．4％、8％。     

Effect of sourdough fermentation on the quality of Chinese Northern-style steamed breads

The effect of spontaneous and lactic acid bacteria (LAB) fermentation (Lactobacillus plantarum (LP), Lactobacillus sanfranciscensis (LS), Lactobacillus brevis (LBr) and Lactobacillus delbrueckii subsp bulgaricus (LB) strains) on the specific volume, crumb texture, volatiles profile and other aspects of steamed bread quality were studied. The addition of sourdough starter significantly decreased dough water absorption, development time and stability while increasing steam bread specific volume. The fermentation with LAB starters reached the desired pH 4 five times faster than the spontaneous starters. Steamed bread leavened with LS and LB starters had the softest crumb texture whereas LP and LBr starters produced the lowest setback viscosity. From the total of 77 compounds identified in sourdough steamed bread, ethanol and 3-methyl-1-butanol were the most abundant. All sourdough steamed breads had 22 compounds in common, which represented about 90% of the total peak area. But unique volatile compounds were present in sourdough steamed bread with different starters.     

Quality characteristics of northern-style Chinese steamed bread prepared from soft red winter wheat flours with waxy wheat flour substitution

Quality characteristics of northern-style Chinese steamed bread (CSB) prepared from two soft red winter (SRW) wheat flours blended with 0–30% waxy wheat flour (WWF) were analyzed to estimate the influence of starch amylose content. The increased proportion of WWF in blends raised mixograph absorption with insignificant changes in protein content and dough strength-related parameters. WWF incorporation generally increased specific volume and crumb softness of CSB. The analysis of covariance revealed that CSB quality attributes were little affected by protein content and dough strength-related parameters, indicating that starch amylose content was largely responsible for the changes in CSB quality. Flour blends with 5–10% WWF, of which starch amylose content was 22.4–24.7%, produced CSB with superior crumb structure compared to other blends, but insignificant changes in surface smoothness, stress relaxation and total score compared to the respective control wheat flours. Flour blends with 15% WWF to produce a starch amylose content of 21.4–22.7% exhibited reduced staling of CSB with total scores comparable to the respective control wheat flours. CSB prepared from blends with more than 10% WWF exhibited a higher soluble starch content, indicative of reduced starch retrogradation, than that prepared from wheat flours without WWF during storage for 3 days.     

Effect of the thermostable xylanase B (XynB) from Thermotoga maritima on the quality of frozen partially baked bread

The effect of the recombinantly produced xylanase B (XynB) from Thermotoga maritima MSB8 on the quality of frozen partially baked bread (FPBB) was investigated. Addition of XynB to wheat flour dough resulted in a significant increase in dough extensibility (L), swelling (G), and a decrease in dough resistance to deformation (P), configuration. Bread crumb characteristics were studied by differential scanning calorimeter (DSC) and dynamic-mechanical analysis (DMA). The results show that addition of XynB leads to improvements in the bread quality of FPBB and retards bread staling compared to the control. The greatest improvements were obtained in specific volume (+35.2%) and crumb firmness (−40.0%). The control FPBB was significantly firmer in texture and higher in amylopectin recrystallization than the bread with XynB. During frozen storage of FPBB with and without XynB for 8 weeks, the crumb firmness increased gradually and the specific volume slightly decreased with the frozen storage time. The ΔH values of freezable water (FW) endothermic transitions increased with frozen storage time for all samples. However, addition of XynB lowered the ΔH values indicating a decrease in FW. Therefore, XynB is useful in improving the quality of FPBB. DMA was also used to monitor the shrinking behavior of the samples. Addition of XynB increased the contraction during chilling but significantly diminished the total shrinking and frozen-state shrinking of the bread crumb during the freezing process.     

Interrelation between mechanical and biological aeration in starch-based gluten-free dough systems

Alternative aeration and gas stabilization strategies are required for the production of starch-based cellular food systems, such as gluten-free bread. In the present study, density and temperature were monitored in mixing experiments without yeast, aiming at maximum mechanical aeration. Additionally, the same trials were performed with subsequent biological aeration, including yeast fermentation and baking. As a result, the gas volume fraction was elevated to 21%, instead of 6% with conventional kneading. Reducing the water content from 120% to 90% (flour/starch weight base) raised dough viscosity and temperature without affecting the state of aeration. The bread volume was strongly influenced by the dough temperature after mixing (R² = 0.98), since it depended on yeast activity. The implemented process is suitable to aerate starch-based dough systems mechanically and enables the production of gluten-free bread with high volume and fine pores.     

Dough and bread made from high- and low-protein flours by vacuum mixing: Part 2. Yeast activity,dough proofing and bread quality

The combined effects of reduced mixer headspace pressure and mixing duration on the yeast activity, proofing and quality of dough and bread made from both high-protein flour (HPF) and low-protein flour (LPF) were addressed in this study. Rheofermentometer analysis showed that a reduction in mixer headspace pressure up to 0.08 MPa did not affect the overall gassing power of yeast in either of the two dough matrices. An increase in mixing duration sped up the mass transfer rate of CO₂ at the initial stage of fermentation, leading to a faster expansion of dough volume at the beginning. Moreover, an increase in mixing time promoted dough development and gas inclusion, which resulted in a increased volume of dough and bread, as well as a softer texture of both baked bread and steamed bread. In general, reduced headspace pressure produced baked bread of smaller volume, denser structure and harder texture. On the other hand, vacuum mixing produced steamed bread with softer texture without significantly changing the bread’s volume and porosity.     

蒲城椽头馍与普通馒头的品质特性比较

为揭示蒲城椽头馍与普通馒头的品质差异,通过测定蒲城椽头馍和普通馒头的pH值、酸度、水分含量、比容、质构特性、扫描电镜图像(SEM)、挥发性物质等品质特性,并采用定量描述分析(QDA)方法进行感官评价,探讨了两者之间的差异性。结果表明,蒲城椽头馍的pH、酸度、水分含量、比容、质构特性与普通馒头差异显著(P0.05);蒲城椽头馍与普通馒头相比,其微观网络结构更紧密,尚有部分清晰可见的淀粉粒;蒲城椽头馍的香气、酸味、咀嚼性等感官特性的强度均高于普通馒头,甜味、弹性等感官特性的强度低于普通馒头;挥发性物质种类比普通馒头多2~10种,蒲城椽头馍的挥发性成分主要是酸类物质,而普通馒头的挥发性物质主要是醇类物质。目前,工厂化还不能完全代替手工椽头馍的生产。