中成药"欢宝止痢胶囊"紫外分光光度的定性检测对比试验

分别采用以蒸馏水,60%乙醇和0.9%盐酸溶液对欢宝止痢胶囊内容物作5000倍稀释,用紫外分光光度法作定性对比检测,经重复试验检测表明用蒸馏水法其最大吸收峰差别＞±1nm,而用60%乙醇法和0.9%盐酸法其最大吸收峰和次吸收峰相差都＜1nm,说明60%乙醇法和0.9%盐酸法都可用作该药定性的质量标准.     

"欢宝止痢胶囊"急性毒性试验

“欢宝止痢胶囊”是贵州省畜牧兽医科研所中兽医药物课题组研制的纯中草药复方提取物制备的胶囊剂，每粒胶囊内含成药０．３g，经临床应用５００余例，其止痢止泻效果满意，实验证明其进入肠道后能显著抑制肠运动，临床推荐用量为畜禽每１０kg体重１粒，为弄清该药是否存在毒付作用，特作本试验，报道如下：１材料与方法１．１材料：昆明种健康小白鼠１２０只，体重１８g±１g、雌雄各半，均购自贵阳医学院实验动物中心，止痢胶囊由课题组提供（批号２００００４０１）。１．２方法：１．２．１将止痢胶囊内容物５０g（约１７０粒）加蒸馏水…     

兽用止痢灵胶囊的一般毒性研究

兽用止痢灵胶囊属新型复方制剂 ,是由哈尔滨广昌畜牧兽医应用技术研究所研制 ,专治仔猪黄白痢疾病。通过 6 70 0多头仔猪初试和 30多万头仔猪中试临床试验 ,兽用止痢灵胶囊对仔猪黄白痢的治疗有效率达 99.5 %～ 99.8% ,预防有效率为 94.7%。为进一步评价其用药的安全性 ,本试验对兽用止痢灵胶囊的急性毒性、蓄积毒性与耐受性及局部刺激性进行了试验研究 ,为临床安全、合理用药提供理论依据。1　材料与方法1 .1　材料1 .1 .1　试验动物　昆明种小白鼠 1 30只 ,雌雄各半 ,体重1 8～ 2 2ｇ ;杂种白色家兔 8只 ,雌雄各半 ,体重 1～ 1 .2ｋｇ。1 …     

止痢宁对仔猪腹泻病的疗效试验

止痢宁由中西药物组成，具有抗菌消炎、收敛止泻的作用。经对仔猪人工复制腹泻病例和自然腹泻病例的治疗，用药1～3次后，治愈率为96．4％。结果表明：止痢宁具有用量少，疗程短，疗效确实，使用方便，且治疗成本低的特点。     

止痢散及其拆方对胃肠运动的影响

为了研究家畜新兽药止痢散及其拆方中多叶勾儿茶、地榆和乌药组方对胃肠运动的影响,评价家畜新兽药止痢散及其拆方的抗腹泻作用并初步探索其抗腹泻的作用机制,采用动物模型观察止痢散及其多叶勾儿茶、地榆和乌药组方的抗腹泻效果,对正常或新斯的明致胃肠痉挛小鼠肠推进、胃残留率的影响,对家兔离体回肠平滑肌收缩活动的影响。结果表明,止痢散及多地组、多地乌组都可极显著减少小鼠腹泻次数(P0.01);同时可显著减少正常小鼠肠推进率和胃排空率(P0.01);但不能显著减少新斯的明致胃肠痉挛模型小鼠肠推进率和胃排空率(P0.05)。止痢散及多地组、多地乌组都可显著减小家兔离体回肠平滑肌收缩张力的变化(P0.01)。综上所述,止痢散及其拆方的抗腹泻作用是与其抑制肠推进和胃排空,减小肠平滑肌收缩张力有关,但都不能对抗痉挛性胃肠运动,止痢散的这一作用与方剂中的多叶勾儿茶、地榆和乌药关系密切,这为止痢散的组方和在临床上的进一步开发利用提供了数据参考。     

兽用止痢、止泻中草药新制剂的药理试验

通过兽医临床疗效观察,筛选并研制出具有止痢、止泻功效的兽用中草药配方制剂,对治疗家畜消化系统疾病:仔猪白痢、仔猪黄痢、家畜胃肠炎及消化不良性腹泻具有独特的疗效。经实验室对该药的药理试验表明:使其药物的止痢、止泻的功效极为显著,这与临床疗效的结果一致。经实验室急性毒性试验表明:该药对受试动物是安全的、可靠的,可用于兽医临床、也可作饲料添加剂。     

中成药"胃康灵散"紫外分光光度定性检测对比试验

本试验分别采用双蒸馏水、60%乙醇和9/1000盐酸溶液对“胃康灵散”作5000倍稀释后,用紫外分光光度法定性对比检测结果为:用蒸馏水法以及60%乙醇法其最大吸收峰值和次吸收峰值经重复试验相差都在±1nm内。表明蒸馏水法和60%乙醇法都可用作该药定性的检测标准。     

"禽宝安"对提高肉鸽生产性能的试验观察

“禽宝安”是一种蛋白水解产物,并经一定的生产工艺,研制而成的新型营养保健剂。它含有十多种氨基酸(包括多种家禽所必需的限制性氨基酸)、多种维生素、微量元素和生物活性因子,具有促进家禽生长、缓解各种应激及提高禽体抗病力及繁殖能力的功效。为验证“禽宝安”对产鸽、乳鸽     

瘦肉精的毒害作用及其试纸快速检测技术

文章综述了"瘦肉精"的非法使用及其综合治理,介绍了"瘦肉精"的毒害作用和检测技术,从设计原理,定性、半定量、定量检测等方面详细阐述了免疫试纸快速检测技术及其在"瘦肉精"检测中的应用。     

The Influence of the Halothane Test on Heart Parameters,Oct-Activity,Acid-Base Balance and Blood Electrolytes in Halothane-Sensitive Pigs and in Pigs Pre Medicated with a β-Blocker (Propranolol)

The heart and liver functions, blood electrolytes and acid-base balance were studies in halothane-sensitive and nonsensitive pigs. An inhibition of the hyperthermia reaction was attempted by an i.v. injection of propranolol. The pulse rate of sensitive pigs was shown to be elevated already at the beginning of halothane narcosis. No other differences between sensitive and nonsensitive pigs were observed from the electrocardiogram. Ectopic beats and arythmia appeared only in 1 halothane-sensitive pig which died soon after the test. The serum OCT-activity showed no abnormalities. Hyperkalemia and acidosis occurred but the Ga⁺⁺ was not elevated in halothane-sensitive animals. The slight elevation in Na⁺ was thought to be caused by hemoconcentration. A propranolol injection only delayed but did not inhibit the halothane reaction.     

Development of a Latex Agglutination Test Using The Major Epitope Domain of Glycoprotein E of Pseudorabies Virus Expressed in <Emphasis Type="Italic">E. coli</Emphasis> to Differentiate Between Immune Responses in Pigs Naturally Infected or Vaccinated with Pseudorabies Virus

A 0.8 kb DNA fragment encoding the major epitope domain of glycoprotein E (gE) of pseudorabies virus (PRV) was inserted downstream of the T7 promoter of an expression vector, pET-28b, to yield the recombinant plasmid pETgE804. After induction by isopropy1-β-D-thiogalactopyranoside (IPTG), a high level expression of fusion protein was obtained. SDS-PAGE and western immunoblotting analysis showed that the fusion protein was 38 kDa and could bind with antisera against PRV. The protein existed mainly in the form of the inclusion body. After being denatured and renatured, the protein was used to prepare the latex antigen. The concentration of antigen, temperature and time for sensitization were optimized. The latex agglutination test (LAT) was able to differentiate sera of PRV-infected pigs from those of gE-deletion vaccine-immunized pigs. The diagnostic specificity and sensitivity of the developed gE latex agglutination test (gE-LAT) were also evaluated by using sets of sera. The diagnostic specificity and diagnostic sensitivity of the gE-LAT were 96.77% and 95.76%, respectively. For comparison between gE-LAT and a commercial blocking enzyme-linked immunosorbent assays (ELISA), 260 serum samples were tested. The coincidence frequency of both assays was 96.94% (252/260). No significant difference was found between the two methods (p>0.05). For comparison between the abilities of gE-LAT and gE-ELISA to detect sera with low titres of gE-specific antibody, 66 sera from 22 pigs were tested. The data indicate that the gE-LAT is of similar sensitivity to gE-ELISA. These results indicate that gE-LAT using recombinant gE might be very useful as a routine screening method for the differential diagnosis of PRV infection.