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Katherine A Damcevski Greg Dojchinov James D Woodman Victoria S Haritos 《Pest management science》2010,66(4):432-438
BACKGROUND: The ethyl formate/carbon dioxide (CO2) formulation Vapormate? is a rapid‐acting fumigant being developed for the control of stored‐grain insects. The effects have been investigated of concentration, exposure times of 1, 3, 24 and 72 h and two grain temperatures, 15 and 25 °C, on its efficacy against mixed‐stage cultures of Sitophilus oryzae (L.) Tribolium castaneum (Herbst) and strongly phosphine‐resistant Rhyzopertha dominica (F.) strain QRD569. RESULTS: High mortalities (≥92%) of mixed‐stage cultures of all three species were obtained when grain was fumigated with the formulation (193 g m?3 ethyl formate) for 1 h. Complete control of R. dominica QRD569 and T. castaneum was achieved with 63 and 76 g m?3 ethyl formate respectively, with exposure for 24 h, whereas mean mortality of S. oryzae was 86% under the same conditions. Mortalities of S. oryzae juvenile stages were significantly lower than adults under the conditions tested, which was due to pronounced tolerance of mid‐stage pupae to the fumigant. Reducing grain temperature from 25 to 15 °C had no effect on insect mortality. CONCLUSION: Ethyl formate/CO2 formulation is highly effective against stored‐grain insects over a range of concentrations and exposure times. Efficacious fumigations were conducted in as little as 1 h, and a strongly phosphine‐resistant R. dominica strain was readily controlled with the fumigant. Copyright © 2009 CSIRO, Australia. Published by John Wiley & Sons, Ltd 相似文献
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Greg Dojchinov Katherine A Damcevski James D Woodman Victoria S Haritos 《Pest management science》2010,66(4):417-424
BACKGROUND: Vapormate? is a cylinderised non‐flammable mixture of ethyl formate (16.7% by weight) and carbon dioxide (CO2) that has been developed as a rapid fumigant of stored grain. Four field trials were undertaken on wheat in 50 t farm silos to demonstrate the feasibility of dynamic application. To assess treatment efficacy, each trial tested mixed stages of Rhyzopertha dominica F. (>11 000), Tribolium castaneum (Herbst.) (>1500) and Sitophilus oryzae (L.) (>13 000) in mesh cages positioned through the centre of the grain bulk and on the grain surface. Ethyl formate and CO2 concentrations were measured in the silo during fumigation and in ambient air outside the 6 m fumigation zone. Application rates of 420, 660 and 940 g m?3 of ethyl formate/CO2 formulation and exposure times of 24, 3 and 72 h, respectively, were examined using wheat of 10.4–11.7% moisture content and grain temperatures between 2 and 32 °C. RESULTS: All life stages of R. dominica and T. castaneum were fully controlled under all conditions tested, and mortality of all life stages of S. oryzae was greater than 98%. CONCLUSION: Dynamic application of vaporised ethyl formate and CO2 to 50 t silos proved safe to operators and rapidly effective against stored‐grain insects on cold to warm grain. Copyright © 2009 CSIRO, Australia. Published by John Wiley & Sons, Ltd 相似文献
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报道了甲酸乙酯对米象[Sitophilus oryzae(Linnaeus)]乙酰胆碱酯酶(AChE)和羧酸酯酶(CarE)的活性影响,甲酸乙酯对米象表现出很高的毒力,25℃下熏蒸处理24 h的LC50为28.65μL/L。亚致死剂量的甲酸乙酯可以使米象成虫体内AChE的比活力显著下降,用20μL/L的浓度处理24 h,AChE的比活力由15.684 nmol/mg.min下降至9.530 nmol/mg.min;酶动力学研究表明,甲酸乙酯可使米象成虫体内AChE的Km值明显增加。对于羧酸酯酶,在活体条件下表现为先抑制后激活的规律,离体条件下则主要表现为抑制作用,且随着药剂浓度的增大抑制率也增大。 相似文献