烯虫酯对烟草甲和烟草粉螟的生物活性

为明确烯虫酯对烟叶仓储害虫烟草甲和烟草粉螟的生物活性, 采用卵卡浸渍法测定烯虫酯对孵化率的影响, 采用浸叶法测定对化蛹率、羽化率和发育历期的影响。结果表明, 烯虫酯对烟草甲和烟草粉螟的致死效果随浓度的升高而增强。烯虫酯可显著降低两种害虫卵的孵化率、幼虫化蛹率及成虫羽化率, 并可明显延长幼虫的发育历期。其中, 5 mg/kg烯虫酯处理烟草甲和烟草粉螟卵7 d的校正死亡率分别为67.2%和50.7%, 处理烟草甲幼虫42 d的校正死亡率为57.1%, 处理烟草粉螟幼虫84 d的校正死亡率为60.1%, 幼虫化蛹率分别为9.6%和3.6%, 成虫不能正常羽化。因此, 5 mg/kg为室内筛选出的可同时防治这两种害虫的烯虫酯适宜浓度。     

Management of five stored-product insects in wheat with pirimiphos-methyl and pirimiphos-methyl plus synergized pyrethrins

Hard red winter wheat was treated with pirimiphos-methyl at 4, 6 and 8 mg kg(-1), synergized pyrethrins at 0.38, 0.75, 1.13 and 1.5 mg kg(-1), and combinations of the two insecticides, to conduct laboratory bioassays against four beetle pests of stored grain, red flour beetle Tribolium castaneum (Herbst), rusty grain beetle Cryptolestes ferrugineus (Stephens), lesser grain borer Rhyzopertha dominica (F), and rice weevil Sitophilus oryzae (L), and one moth pest, Indianmeal moth Plodia interpunctella (Hubner). Beetle adults and P interpunctella larvae survived well on control wheat, producing a large number of progeny (65-1037 insects per container). Kernel damage in control wheat among the insect species ranged from 9 to 99%. On pirimiphos-methyl-treated wheat, mortality of R dominica adults was > or =72%, but that of the other beetle species and P interpunctella larvae was 100%. Progeny were not produced on pirimiphos-methyl-treated wheat, and the kernel damage was negligible (< or =1%). Synergized pyrethrins were ineffective against the five insect pests. Pirimiphos-methyl combined with synergized pyrethrins was not superior to pirimiphos-methyl alone against the five insect pests. Pirimiphos-methyl is not registered in the USA for use on wheat, but our results suggest that it could be a viable grain protectant at rates of 4-8 mg kg(-1).     

Contribution of contact toxicity and wheat condition to mortality of stored-product insects exposed to spinosad

Spinosad, a reduced-risk commercial insecticide derived from a bacterial fermentation product, possesses both contact and oral toxicities against insects. Contact toxicity of spinosad to adults of Rhyzopertha dominica (F), Sitophilus oryzae (L), and Tribolium castaneum (Herbst) was evaluated by exposure for 24 or 48 h to treated glass Petri dishes. Adults were exposed to different deposits (0.001-0.79 mg cm(-2)) of spinosad in 24-h tests and to deposits of 0, 0.0016 and 0.016mg cm(-2) in 48-h tests. Rhyzopertha dominica was most susceptible to spinosad in 24- and 48-h tests, followed by S. oryzae, and T. castaneum. The 24-h LD50 values were 0.0004, 0.077 and 0.189mg cm(-2) for R. dominica, S. oryzae, and T. castaneum, respectively. All R. dominica adults were dead following 48 h exposure to both spinosad deposits, whereas mortality of S. oryzae and T. castaneum ranged from 10 to 85% and 12 to 48%, respectively. Rhyzopertha dominica, T. castaneum, and O. surinamensis adults were exposed for 14 days to whole wheat, cracked wheat and wheat flour treated with 0, 0.1 and 1.0 mg kg(-1) of spinosad. Rhyzopertha dominica adults were highly susceptible to spinosad, followed by O. surinamensis and T. castaneum. Immatures (eggs and larvae) of T. castaneum and O. surinamensis exposed for 14 days were more susceptible on spinosad-treated whole wheat than on treated cracked wheat and wheat flour. This is the first report documenting contact activity of spinosad, and the effect of grain condition on spinosad toxicity, to stored-product insects.     

贵州烟仓害虫天敌种类及优势种的生物学特性

对贵阳地区的烟仓害虫天敌种类进行了调查.结果表明,其天敌共有11种,隶属3纲、7目、10科;优势种天敌为麦蛾柔茧蜂、米象金小蜂.麦蛾柔茧蜂完成一代夏季为9～13d,秋季为13～18d,一年发生世代数可达10代以上;成虫寿命12～17d.将采集的烟草粉斑螟幼虫于室内饲养,麦蛾柔茧蜂的出蜂率为46.0%;在烟仓的墙壁、过道和烟包表层,麦蛾柔茧蜂对烟草粉斑螟的平均寄生率达61.0%.米象金小蜂完成一代需30～33d,发生世代数3代;成虫寿命为10～15d.在烟仓,该蜂对烟草甲幼虫和蛹的寄生率分别为24.53%～33.70%、42.59%～61.64%.米象金小蜂对寄主烟草甲的跟随现象明显.     

Influence of phosphine on hatching of Cryptolestes ferrugineus (Coleoptera: Cucujidae), Lasioderma serricorne (Coleoptera: Anobiidae) and Oryzaephilus surinamensis (Coleoptera: Silvanidae)

The hatching and mortality response of 0- to 48-h-old eggs of field strains of the stored-product insects Cryptolestes ferrugineus (Stephens), Lasioderma serricorne (F) and Oryzaephilus surinamensis (L) following phosphine fumigation for 24, 48 or 120 h at 27 (+/- 2) degrees C was investigated. Hatching was delayed and reduced in the first few days in a phosphine-resistant strain of C ferrugineus that was treated with 2.0-7.0 mg litre(-1) doses for 48 h (5-80% mortality) and with 1.0-2.0 mg litre(-1) for 120 h (44-84% mortality). In both the exposures there were significant increases in hatching on later days when compared with the corresponding controls. Developmental delay was, however, not evident in susceptible strains of C ferrugineus, L serricorne and O surinamensis that were exposed to phosphine for 24 h.     

Combined treatments of spinosad and chlorpyrifos-methyl for management of resistant psocid pests (Psocoptera: Liposcelididae) of stored grain

The combined efficacy of spinosad and chlorpyrifos-methyl was determined against four storage psocid pests belonging to genus Liposcelis. This research was undertaken because of the increasing importance of these psocids in stored grain and the problem of finding grain protectants to control resistant strains. Firstly, mortality and reproduction were determined for adults exposed to wheat freshly treated with either spinosad (0.5 and 1 mg kg(-1)) or chlorpyrifos-methyl (2.5, 5 and 10 mg kg(-1)) or combinations of spinosad and chlorpyrifos-methyl at 30 degrees C and 70% RH. There were significant effects of application rate of spinosad and chlorpyrifos-methyl, both individually and in combination, on adult mortality and progeny reduction of all four psocids. Liposcelis bostrychophila Badonnel and L. decolor (Pearman) responded similarly, with incomplete control of adults and progeny at both doses of spinosad but complete control in all chlorpyrifos-methyl and combined treatments. In L. entomophila (Enderlein) and L. paeta Pearman, however, complete control of adults and progeny was only achieved in the combined treatments, with the exception of spinosad 0.5 mg kg(-1) plus chlorpyrifos-methyl 2.5 mg kg(-1) against L. entomophila. Next, combinations of spinosad (0.5 and 1 mg kg(-1)) and chlorpyrifos-methyl (2.5, 5 and 10 mg kg(-1)) in bioassays after 0, 1.5 and 3 months storage of treated wheat were evaluated. The best treatment was 1 mg kg(-1) of spinosad plus 10 mg kg(-1) of chlorpyrifos-methyl, providing up to 3 months of protection against infestations of all four Liposcelis spp. on wheat.     

气调胁迫下3种中药材储藏期害虫谷胱甘肽转移酶活性研究

研究了药材甲(Stegobium paniceum)、烟草甲(Lasioderma serricorne)和咖啡豆象(Araecerus fasciculatus)3种中药材储藏期害虫谷胱甘肽转移酶的酶活性,探讨了该酶在CO2气调胁迫下的应激反应。结果表明,药材甲、烟草甲和咖啡豆象的谷胱甘肽转移酶比活力分别为(0.339 2±0.015 5)、(0.966 0±0.259 3)nmol/(min&#8226;mg)和(0.370 2±0.032 8)nmol/(min&#8226;mg)。CO2气调对3种昆虫GSTs的影响研究结果表明,在CO2气调处理3、6、9 h时,药材甲、烟草甲和咖啡豆象成虫的GSTs酶比活力均有一定程度的升高。可见,CO2气调亚致死处理对3种实验昆虫GSTs有一定的诱导激活作用。研究结果对于储藏物害虫气调杀虫机制的分析有指导意义。     

Monitoring of beet armyworm resistance to spinosad and methoxyfenozide in Mexico

BACKGROUND: Resistance to spinosad and methoxyfenozide has been studied in several insect pests, but there is a lack of information on Spodoptera exigua (Hübner) in Mexico. Therefore, evidence for the development of resistance in this pest to both compounds was examined. The effects of methoxyfenozide on reproductive parameters of S. exigua adults were also determined.RESULTS: Third instars from a field population were exposed for 24 h to the LC(50) of spinosad or methoxyfenozide for over six generations (G(2)-G(7)). No significant reduction in susceptibility to either compound was detected for up to five generations. In G(7), LC(50) values for insects exposed to spinosad and methoxyfenozide were respectively 2.75-fold and 1.25-fold greater than for G(1) larvae. Oral treatment with methoxyfenozide reduced the fecundity and fertility of G(7) adults, confirming sublethal effects on reproduction. Finally, five populations (Se-La Floriza, Se-Lazareto, Se-Bachigualato, Se-Los Agustinos and Se-Villa de Arista) of S. exigua were collected from fields in three states of Mexico for resistance monitoring to spinosad and methoxyfenozide. With the exception of Se-Villa de Arista, the other populations showed significant resistance to spinosad, with resistance ratios between 16- and 37-fold, compared with a susceptible laboratory colony. In contrast, only one population (Se-Lazareto) showed significant resistance to methoxyfenozide (13-fold).CONCLUSION: Resistance management programmes should be established, particularly in areas where S. exigua has developed resistance to spinosad. Copyright (c) 2008 Society of Chemical Industry.     

五种药剂对黏虫卵的室内触杀效果及胚胎发育的影响

为有效防控黏虫Mythimna separata,选用灭多威、苦参·印楝素、苏云金芽胞杆菌Bacillus thuringiensis、甲维盐和高效氯氟氰菊酯5种药剂对其卵进行室内触杀试验,比较这5种药剂对黏虫卵和初孵幼虫的触杀效果及对卵的毒力,测定不同药剂处理后黏虫卵初孵幼虫存活率、发育历期、化蛹率、蛹重和羽化率;并通过冷冻切片技术观察不同药剂处理后黏虫卵的外部形态和胚胎发育情况。室内触杀试验结果表明,苦参·印楝素浓度大于0.1 mg/L时,对产后不同时间黏虫卵的校正死亡率介于42.89%～97.34%之间,苏云金芽胞杆菌浓度为0.01 mg/L时对产后72 h黏虫卵的校正死亡率已达71.99%;各浓度苦参·印楝素和苏云金芽胞杆菌处理产后24、48和72 h黏虫卵后,其初孵幼虫校正死亡率介于39.02%～83.59%之间。苦参·印楝素对产后24、48 h黏虫卵的毒力最大,致死中浓度LC_(50)分别为0.018、0.109 mg/L;苏云金芽胞杆菌对产后72 h黏虫卵的毒力最大,LC_(50)为0.004 mg/L。黏虫卵经5种药剂处理后,幼虫化蛹率、蛹重和羽化率分别介于91.07%～93.64%、0.272～0.284 g和86.60%～87.78%之间,与对照差异不显著,幼虫存活率较对照显著下降43.02%～60.27%,幼虫发育历期较对照延长0.23～0.88 d,其中灭多威、苦参·印楝素、苏云金芽胞杆菌处理的幼虫发育历期较对照显著延长。苦参·印楝素、苏云金芽胞杆菌使胚胎无法形成正常胚带,同时会使已发育的虫体出现蜷缩、腹部皱缩;高效氯氟氰菊酯会延缓胚胎发育时间,使已形成的虫体畸形或使其肠道断裂;甲维盐影响虫体对营养物质的吸收,造成虫体萎缩,使其不能正常孵化。在黏虫卵防治中推荐使用苏云金芽胞杆菌和苦参·印楝素。     

Responses of Corcyra cephalonica (Stainton) to pirimiphos-methyl, spinosad, and combinations of pirimiphos-methyl and synergized pyrethrins

Field control failures with pirimiphos-methyl against the rice moth, Corcyra cephalonica (Stainton), in Weslaco, Texas, USA, led us to investigate the susceptibility of this particular strain to pirimiphos-methyl, spinosad, pyrethrins synergized with piperonyl butoxide, and pirimiphos-methyl combined with synergized pyrethrins. In laboratory bioassays, 50 eggs of C cephalonica were exposed to untreated and insecticide-treated corn and sunflower seeds to determine larval survival after 21 days, egg-to-adult emergence after 49 days, and larval damage to seeds at both exposure periods. Pirimiphos-methyl at both 4 and 8 mg kg(-1) did not prevent larval survival or egg-to-adult emergence of C cephalonica on either corn or sunflower seeds, and seed damage was evident at both rates. The C cephalonica strain was highly susceptible to spinosad at 0.5 and 1 mg kg(-1). At both spinosad rates, reduction in larval survival, egg-to-adult emergence, and seed damage relative to the control treatment was > or = 93% on both corn and sunflower seeds. Pirimiphos-methyl and spinosad were generally more effective against C cephalonica on corn than sunflower seeds. The C cephalonica strain was completely controlled on corn treated with 1.5 mg kg(-1) of pyrethrins synergized with 15 mg kg(-1) of piperonyl butoxide. Many larvae survived and became adults on corn treated with synergized pyrethrins at < or = 0.75 mg kg(-1). Corn treated with pirimiphos-methyl at 4, 6 or 8 mg kg(-1) in combination with 0.38 to 1.5 mg kg(-1) of synergized pyrethrins reduced larval survival by > or = 95%, egg-to-adult emergence by > or = 97%, and seed damage by > or = 94%. Our results suggest that the C cephalonica strain can be controlled on corn by combining pirimiphos-methyl with synergized pyrethrins or with synergized pyrethrins at the labeled rate. Although spinosad is not currently labeled for use on stored corn and sunflower seeds, it appears to be effective against C cephalonica on both commodities at very low rates.     

红嘴蓝鹊的饲养、驯化及防治松毛虫的效果研究

红嘴蓝鹊是天目山区的留鸟,食性杂,能捕食30多种昆虫,食虫量大,棚养鸟每天能吃虫50～60克,约松毛虫幼虫70～80条。每只蓝鹊每年可控制30亩松林的害虫。该鸟有一定的群居性,易驯化,在浙江地区是驯养、治虫的最佳鸟种,且观赏性好。     

太阳能光陷阱捕虫器对花生田昆虫的诱捕效果

为建立绿色高效的花生田害虫灯光诱控技术,于2019年在湖北省孝感市大悟县河口镇群建村进行试验,测定光源A（波长365 nm）和光源B（波长390 nm）太阳能光陷阱捕虫器诱捕到的花生田地下害虫、天敌昆虫和中性昆虫的类群和数量,并分析不同光源和天气对太阳能光陷阱捕虫器诱捕效果的影响。结果表明,2种光源太阳能光陷阱捕虫器共诱捕昆虫106 899头,其中地下害虫以丽金龟科和鳃金龟科昆虫居多,分别为53 338头和22 993头;诱捕的天敌昆虫和中性昆虫中分别以步甲科昆虫和水龟虫科昆虫居多,分别为1 495头和4 866头。光源A诱捕的金龟甲总科害虫、叩甲科害虫、天敌昆虫和中性昆虫数量与光源B的诱捕量均差异不显著,但光源A诱捕的蝼蛄科害虫数量显著高于光源B;晴天诱捕的金龟甲总科害虫、叩甲科害虫、天敌昆虫和中性昆虫数量与在阴天的诱捕量差异不显著,但晴天诱捕的蝼蛄科害虫数量显著高于阴天的诱捕量。表明太阳能光陷阱捕虫器对花生田害虫的诱捕效果好,对天敌昆虫和中性昆虫杀伤力小,可作为一种绿色防控手段防治花生田害虫。     

溴虫氟苯双酰胺对小菜蛾的毒力及相关酶活性的影响

溴虫氟苯双酰胺是一种全新的双酰胺类杀虫剂,主要用于防治鳞翅目、鞘翅目、白蚁以及蚊蝇等害虫。为了明确溴虫氟苯双酰胺对小菜蛾的生物活性及解毒酶活性的影响,本研究选择氯虫苯甲酰胺为对照药剂,采用叶片药膜法测定了溴虫氟苯双酰胺对小菜蛾的生物活性,并测定了亚致死剂量(LC20、LC40)的溴虫氟苯双酰胺和氯虫苯甲酰胺对小菜蛾多功能氧化酶、谷胱甘肽S-转移酶和羧酸酯酶活性的影响。结果表明,溴虫氟苯双酰胺与氯虫苯甲酰胺对小菜蛾的生物活性相当,两者对小菜蛾的LC50分别为0.042和0.056μg/mL。与氯虫苯甲酰胺相似,亚致死剂量的溴虫氟苯双酰胺能够诱导小菜蛾体内羧酸酯酶活性升高,但溴虫氟苯双酰胺对多功能氧化酶和谷胱甘肽S-转移酶活性没有显著影响。     

Phosphine resistance, respiration rate and fitness consequences in stored-product insects

Resistance to fumigants has been frequently reported in insect pests of stored products and is one of the obstacles in controlling these pests. The authors studied phosphine resistance and its physiological basis in adult insects of 12 populations of Tribolium castaneum (Herbst) (Tenebrionidae), ten populations of Rhyzopertha dominica (F.) (Bostrichidae) and eight populations of Oryzaephilus surinamensis L. (Silvanidae) from Brazil, and the possible existence of fitness costs associated with phosphine resistance in the absence of this fumigant. The bioassays for the detection of phosphine resistance followed the FAO standard method. The production of carbon dioxide and the instantaneous rate of population increase (r(i)) of each population of each species were correlated with their resistance ratios at the LC(50). The resistance ratio at LC(50) in T. castaneum ranged from 1.0- to 186.2-fold, in R. dominica from 2.0- to 71.0-fold and in O. surinamensis from 1.9- to 32.2-fold. Ten populations of T. castaneum, nine populations of R. dominica and seven populations of O. surinamensis were resistant to phosphine. In all three species there was significant association (P < 0.05) between respiration rate and phosphine resistance. The populations with lower carbon dioxide production showed a higher resistance ratio, suggesting that the lower respiration rate is the physiological basis of phosphine resistance by reducing the fumigant uptake in the resistant insects. Conversely, populations with higher r(i) showed lower resistance ratios, which could indicate a lower rate of reproduction of the resistant populations compared with susceptible populations. Thus, management strategies based on the interruption of phosphine fumigation may result in reestablishment of susceptibility, and shows good potential for more effective management of phosphine-resistant populations.     

纯磷化氢熏蒸对桃小食心虫的毒力作用

桃小食心虫在我国温带水果主产区广泛分布,严重威胁水果出口贸易。为明确纯磷化氢熏蒸处理桃小食心虫的可能性,本文系统研究了纯磷化氢在不同温度条件下对桃小食心虫的毒力。耐受性分析结果表明,桃小食心虫各虫态对磷化氢的耐受力有很大差别,1~2龄幼虫耐受性最弱,5龄老熟幼虫耐受性最强。室内毒力试验结果表明,25℃,0.14~0.70mg/L磷化氢熏蒸5龄老熟幼虫81~199h或15℃,0.28~0.84 mg/L磷化氢熏蒸136~166h,桃小食心虫死亡率可达99%;在25℃和15℃下,试虫达到一定死亡率的气体浓度、时间乘积(CT)关系式分别为k=C0.5 T和k=C0.2 T,说明在本试验所取浓度范围内,处理时间是影响磷化氢对桃小食心虫老熟幼虫毒力作用的主要因素,相同条件下高温比低温更有利于磷化氢熏蒸杀虫。本文首次对不同温度条件下纯磷化氢熏蒸桃小食心虫的毒力作用进行了系统研究,明确了纯磷化氢熏蒸技术在水果携带桃小食心虫的检疫处理中极具应用前景。     

Mechanism of resistance to spinosyn in the tobacco budworm, Heliothis virescens

Topical laboratory selection of tobacco budworm larvae, Heliothis virescens, with technical spinosad for multiple generations resulted in larvae 1068-fold resistant to topical applications of the insecticide and 316.6-fold resistant to insecticide treated diet as compared to the parental strain. The penetration of 2′-O-methyl[¹⁴C]spinosyn A across the cuticle of the susceptible (parental) and selected (resistant) tobacco budworms increased with time 3-12 h after application. A trend of reduced penetration in the resistant strain was found but the differences were not statistically significant. 2′-O-methyl[¹⁴C]spinosyn A when injected into the hemocoel was not metabolized 96 h after treatment in both the susceptible and resistant strain, suggesting that a change in metabolism was not the mechanism of resistance. Electrophysiological studies indicated that dose-dependent spinosyn A-induced currents occurred in neurons from spinosyn resistant and susceptible (adult) tobacco budworms. At both 10 and 100 nM spinosyn A, however, the amplitude of these currents in the resistant insects was significantly smaller than the amplitude of currents observed from neurons from susceptible tobacco budworm adults. This suggests that neurons from resistant insects have decreased sensitivity to spinosyn A. However, the reduced inward currents in the resistant strain may or may not be related to the mode of action of the spinosyns. No statistically significant cross-resistance was noted for the spinosad resistant tobacco budworms for topical applications of permethrin (Pounce^®), profenofos (Curacron^®), emamectin benzoate (Denim^®), or indoxacarb (Steward^®). A statistically significant reduction in susceptibility to acetamiprid (Mospilan^®) in artificial diet as determined from a resistance ratio of 0.482 was found.     

用初孵幼虫监测棉红铃虫对杀虫剂的抗性

本文通过试验提出了一种准确、简易、快速的棉红铃虫抗药性测定方法－初孵幼虫滤纸药膜法。并利用这种方法测定了棉红铃虫田间种群对甲基对硫磷和溴氰菊酯的抗性，结果发现安徽安庆、湖北江陵和天门３个种群对溴氰菊酯分别产生了２０３．８、３．７和４．２倍抗性，安庆种群还对甲基对硫磷产生４．４倍的低水平抗性，而浙江慈溪种群对甲基对硫磷的敏感性正在降低，ＬＤ５０值增高２．４倍。这些结果与当地的实际用药情况相符，说明初     

Fenoxycarb,an insect growth regulator with juvenile hormone activity: A candidate for Heliothis virescens (F.) control on cotton

Tobacco budworm eggs, deposited on cotton plants (Gossypium hirsutum L.) treated with fenoxycarb, do not produce viable larvae. The insects die either at the end of embryonic development or during 1st-2nd larval instars, even when the larvae hatching on the treated leaves are transferred to untreated plants. This ovo-larvicidal activity and indications of good foliar persistence make fenoxycarb a promising candidate for the control of tobacco budworm on cotton. This type of application could open new aspects for the control of lepidopterous pests with this novel compound.     

多杀菌素对甜菜夜蛾多酚氧化酶和羧酸酯酶的影响

报道了多杀菌素对甜菜夜蛾Spodopetera exigua(Hübner)多酚氧化酶(PPO)和羧酸酯酶(CarE)的影响, 多杀菌素对甜菜夜蛾表现了很高的毒力,对三龄幼虫的LC50值为0.80 mg/L。离体条件下,1.0×10-3~0.5 mg/L多杀菌素对甜菜夜蛾多酚氧化酶的抑制率超过50％,且表现为随药剂浓度的增加抑制能力增强的趋势。活体条件下,0.1~0.8 mg/L多杀菌素在处理的早期诱导虫体内多酚氧化酶的活性增加,但12 h后却显著抑制多酚氧化酶的活性。1.0×10-3~1.0 mg/L多杀菌素在离体条件下对羧酸酯酶不表现任何抑制作用,但活体条件下同样能诱导虫体内羧酸酯酶活性增强。     

高效液相色谱法检测烟草中噻虫嗪残留及消解动态

建立了高效液相色谱法检测鲜烟叶和干烟叶中噻虫嗪残留量的方法。样品经乙腈提取,Cleanert NH2-SPE固相萃取柱净化,高效液相色谱-紫外检测器(HPLC-UV)检测,外标法定量。结果表明:在0.01~5 mg/kg添加水平下,鲜烟叶中噻虫嗪的平均回收率为92%~95%,相对标准偏差(RSD)为4.5%~9.2%,定量限(LOQ)为0.01 mg/kg;在0.05~5 mg/kg添加水平下,干烟叶中噻虫嗪的平均回收率为91%~94%,RSD为5.9%~6.8%,LOQ为0.05 mg/kg。采用所建立的方法,测定了山东、湖南2年2地烟叶样品中噻虫嗪的消解动态及最终残留量。结果表明:噻虫嗪在烟叶中的半衰期为1.3~8.1 d;按照噻虫嗪有效成分用量18.9和28.4 g/hm2,于烟草现蕾初期-成熟期对水喷雾施药2~3次,距末次施药7 d后,山东、湖南2年2地烟叶样品中噻虫嗪残留量为0.05~0.80 mg/kg,均低于国际烟草合作研究中心(CORESTA)指导性残留限量标准规定的MRL值(5.0 mg/kg)。