小尾寒羊血清钙磷含量的测定

对青海省乐都县小尾寒羊的血清元机瞵和四项血清钙指标进行了测定.结果表明:血清无机磷含量为1.21mmd/L,血清总钙含量为2.53 mmol/L,血清结合钙含量为1.62mmol/L,血清游离钙含量为0.93mmol/L,血清游离钙百分率为36.76%.     

山羊五项血清无机离子浓度测定

对海南州共和县７８只山羊的五项血清无机离子浓度进行了测定。结果，血清钾为４．７８±０．５８ｍｍｏｌ／Ｌ，血清钠为１４１．３４±３．４８ｍｍｏｌ／Ｌ，血清氯为１１２．１９±３．７１ｍｍｏｌ／Ｌ，血清钙为２．２５±０．１６ｍｍｏｌ／Ｌ，血清无机磷为１．６６±０．４２ｍｍｏｌ／Ｌ。     

血清3型鸭甲型肝炎病毒阳性血清的制备

通过使用血清3型鸭甲型肝炎疫苗免疫SPF鸡,制备了一批血清3型鸭甲型肝炎诊断用阳性血清,并对其进行了无菌、支原体和特异性检验以及中和效价测定。结果表明,该血清无菌检验合格,无支原体污染,特异性良好,无禽类常见13种外源病毒抗体,血清中和效价为10-2.8。本研究为鸭甲型肝炎病毒血清学鉴定及相关研究提供了重要的物质基础。     

野仔猪血清钙指标的测定

对青海省多巴镇猪场的12头野仔猪的4项血清钙指标进行了测定。结果表明:血清总钙,2.26±0.29 mmol/L;血清结合钙,1.71±0.15 mmol/L;血清游离钙,0.55±0.21 mmol/L;血清游离钙百分率,23.69%±6.89%。且公、母仔猪间4项钙指标差异不显著(P>0.05)。40日龄仔猪血清总钙、结合钙均显著高于60日龄仔猪(P<0.05)。     

柴达木黄牛四项血清钙指标测定

对８５头柴达木黄牛的上项血清钙指标测定结果表明，犊牛的血清游离钙含量和淳离钙百分率显著地高于成年牛（Ｐ〈０．０１），而血清结合钙含量显著地低于成年牛（Ｐ〈０．０１）。     

中药血清药理学的研究方法及其应用

综述了中药血清药理学研究中对动物的选择、灌胃次数与时间间隔、给药剂量、含药血清的采集、含药血清是否灭活等问题的解决方法,介绍了中药血清药理学方法在国内临床研究中的应用现状.     

赖氨酸对梅花鹿血清生化指标及血清游离氨基酸的影响

研究在低蛋白日粮中添加赖氨酸对梅花鹿仔鹿血清生化指标及血清游离氨基酸的影响,为进一步研究日粮中添加限制性氨基酸对梅花鹿的影响提供参考。将24头健康的梅花鹿仔鹿,随机分成3组(Ⅰ、Ⅱ、Ⅲ)。Ⅰ组饲喂粗蛋白水平为17.60%的日粮,Ⅱ组饲喂粗蛋白水平为14.37%的日粮并添加0.3%赖氨酸,Ⅲ组饲喂粗蛋白水平为17.60%的日粮并添加0.3%赖氨酸。为了测定其血清生化指标、血清游离氨基酸,在试验结束时采血。结果表明:Ⅲ组血清中白蛋白(ALB)含量显著高于Ⅰ组(P0.05),除血清中白蛋白(ALB)含量外,梅花鹿仔鹿各组血清中总蛋白(TP)、球蛋白(GLOB)、尿素氮(BUN)、葡萄糖(GLU)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(AKP)含量均无显著差异(P0.05);各组血清游离氨基酸中谷氨酸(Glu)、丝氨酸(Ser)、缬氨酸(Val)、异亮氨酸(Ile)、亮氨酸(Leu)、赖氨酸(Lys)含量差异显著(P0.05),其中Ⅱ组含量最低,除亮氨酸(Leu)外Ⅰ组含量最高。结果显示,在低蛋白日粮中添加赖氨酸,除对血清白蛋白(ALB)含量有影响外,对其他血清生化指标无影响;日粮蛋白质水平为17.60%,赖氨酸添加量为0.3%时,Ⅲ组血清中游离氨基酸天冬氨酸(Asp)、甘氨酸(Gly)、组氨酸(His)、精氨酸(Arg)、苏氨酸(Thr)、脯氨酸(Pro)、蛋氨酸(Met)含量与Ⅰ组的差异不显著(P0.05)。本试验范围内日粮蛋白水平为14.37%时,赖氨酸适宜添加量在1.06%~1.36%之间。     

饲料铜水平对生长猪血清铜、血清锌和血清铁的影响

选 60头生长白猪 ( 1 9.2 6± 1 .2 0 kg)随机分成 6组 ,每组公母各半 ,使用硫酸铜作为铜源 ,在饲料中添加不同浓度的铜 ,对照组铜添加浓度为 0 mg/kg,高铜试验各组添加浓度为 1 0 0、1 5 0、2 0 0、2 5 0和 30 0 mg/kg。试验期为 80 d,期间每隔 2 0 d采血一次 ,检测血清铜、血清铁和血清锌的含量。分析结果显示 :高铜饲料可明显提高血清铜含量 ,降低血清锌含量 ,但血清铁无显著性变化。第 2 0 d高铜各组平均血清铜比对照组提高 1 7.7% ( P<0 .0 5 ) ,第 40 d提高 2 0 .2 % ( P<0 .0 5 ) ,第 60 d提高 8.6% ( P>0 .0 5 ) ,第 80 d提高 31 .5 % ( P<0 .0 1 ) ,30 0 mg/kg高铜组与对照组比较差异显著 ( P<0 .0 5 )。血清锌第 2 0 d和 40 d,高铜各组平均比对照组降低 1 1 .6%和 1 0 .3%。     

杜洛克杂种仔猪四项血清钙指标的研究

对青海省湟中县多巴镇猪场的20头杜洛克(父本)杂种仔猪的四项血清钙指标进行了测定。结果为:杜洛克二元杂交仔猪,血清总钙,2.40±0.12mmol/L;血清结合钙,1.57±0.20mmol/L;血清游离钙,0.85±0.19 mmol/血清游离钙百分率,35.27±7.64%。杜洛克三元杂交仔猪,血清总钙,2.45 0.24mmol/L;血清结合钙,1.66±0.13mmol/L;血清游离钙,0.80±0.22mmol/L;血清游离钙百分率,32.12±6.64%。且公、母仔猪间四项钙指标差异不显著(P>0.05)。     

大通牦牛血清碱性磷酸酶及钙磷含量的测定

本试验对青海省大通种牛场的35头健康大通牦牛的血清碱性磷酸酶和血清钙、磷含量进行了测定,结果为：血清碱性磷酸酶的含量在45.15±3.41IU·L-1,血清钙含量在2.49±0.53 mmol·L-1,血清无机磷含量在1.98±0.21 mmol· L-1,均处于正常值范围内;公牦牛、母牦牛之间无显著性差异（P＞0.05）.     

Prediction of Serum IgG1 Concentration in Holstein Calves Using Serum Gamma Glutamyltransferase Activity

We examined the relationship between serum gamma glutamyltransferase (GGT) activity and serum gamma immunoglobulin G (IgG1) concentration in Holstein calves. Blood samples were collected from calves aged 1 to 3 days. A follow-up sample was obtained from each calf 2, 7, or 15 days after the initial sampling. Serum GGT activity and lgG1 concentration were measured. Regression models were used to predict lgG1 concentration as a function of age and serum GGT activity measured 2, 7, or 15 days later.
Serum GGT activity and calf age at the time of the second sample were directly related to serum IgG1 concentration in the initial sample in calves aged 3 to 17 days ( r = .54) and in calves aged 3 to 10 days ( r = .63). Models were used to estimate the serum GGT activity equivalent to a serum IgG1 concentration of 1,000 mg/dL. One-day-old calves should have serum GGT activities > 200 IU/L. Four-day-old calves should have serum GGT activities > 100 IU/L. One-week-old calves should have serum GGT activities > 75 IU/L. Calves with serum GGT activities < 50 IU/L should be classified as having failure of passive transfer.     

Concentration of Serum Transferrin in Sick Horses and its Relationship to Serum Albumin Content

Studies of transferrin (Tf) concentration in sera of sick horses were carried out using Mancini’s immunodiffusion technique. Relative values against a chosen reference serum were determined for a total of 112 horses.Horses with acute infections had Tf values significantly below the normal. The lowest individual Tf value in this group (46%) was found in a six-months-old foal with temperature 41°C and watery diarrhoea.Horses suffering from acute laminitis also had decreased Tf values. The lowest value in the whole material (45%) was found in a horse belonging to this group.There was a positive correlation between the Tf value and the albumin content in serum.     

酒精阳性乳患牛血清及乳清离子和微量元素分析

通过检测不同酒精阳性乳血清和乳清中Ca²⁺、Mg²⁺、Na⁺、K⁺浓度及Cu、Zn、Fe、Mn含量,探讨其与酒精阳性乳的关系,结果显示,酒精阳性乳患牛体内的微量元素Zn、Cu和Fe代谢紊乱,会导致抗氧化酶的活性降低、毒性产物含量升高而使自由基代谢平衡紊乱,且Zn、Cu含量的缺乏会降低机体的免疫力;患牛乳中过多的Ca²⁺、Mg²⁺中和酪蛋白微胶粒表面的电荷,导致酪蛋白稳定性下降,致使乳胶体聚集能力增强;这些都易导致酒精阳性乳的形成。     

犊牛血清的检验

对我厂外购4个不同厂家的46批血清分别进行了无菌检验、细胞培养观察和口蹄疫抗体检测。结果表明,有12批出现细菌污染;1批细胞培养贴壁分裂慢,形态差,不符合细胞生长要求;所有样品均为口蹄疫抗体阴性。     

Serum haptoglobin concentration in cattle

To obtain a basal concentration of serum Haptoglobin (Hp) in cattle in Taiwan, Hp concentrations were measured from serum samples collected from 10 healthy heifers, every week for one year. The values were also compared with those collected from 15 cows diagnosed with postpartum metritis. The heifers were successfully impregnated by artificial insemination six months after the tests. Hp concentrations were also measured in the serum collected from 11 other cows within 3 weeks after parturition. The Hp assay developed in this study gave a good correlation (r=0.893)with Western blotting. The Hp concentration of 454 serum samples from the 10 heifers had a mean value of 83.6 +/- 34.1 mg/l, and there was no significant difference among individual heifers. The basal value of Hp in heifers was calculated as less than 73.6 mg/l. No significant difference in Hp concentration was observed among the 10 heifers during cold and warm seasons (19.8 +/- 2.2 degrees C vs 27.3 +/- 1.4 degrees C), or before and after pregnancy. The mean serum Hp concentration from cows suffering from postpartum reproductive disorders was 1133.5 +/- 627.1 mg/l, which was significantly greater than the serum of healthy heifers and postpartum cows (104.6 +/- 61.0 mg/l) (P<0.05). These results demonstrate that Hp concentration may be a useful indicator for cows with postpartum reproductive disorders.