一例犬吉氏巴贝斯虫病的诊治

3岁雄性贵宾犬,体重3.20 kg,免疫完全,未绝育,从未进行过体内外驱虫,院内散养。主诉最近2周发现犬的精神状态渐差,进食量逐渐减少。为了对其进行诊治,采用体格检查、血液学检查以及影像学检查的方法进行诊断,并根据诊断结果进行治疗,结果表明,该犬感染吉氏巴贝斯虫且并发肝脏损伤,通过采取输血、驱虫等治疗措施,患犬的临床症状消失,镜检外周血虫体消失,治疗后未见复发。说明临床上可通过血液学检测对犬的吉氏巴贝斯虫感染进行确诊,且输血结合驱虫治疗可有效治疗该病。     

一例犬吉氏巴贝斯虫病的诊治

犬巴贝斯虫病是由犬巴斯虫、吉氏巴贝斯虫和韦氏巴贝斯虫3种病原引起的一种在临床上表现高热、严重贫血、黄疸、血红蛋白尿和脾肿大的传染病,此病具有一定的季节性,多发生在5~9月,经蜱虫传播的血液寄生虫病。现将笔者所诊治的犬巴贝斯虫病的病例介绍如下,以求与广大同行交流。     

1例犬吉氏巴贝斯虫病继发胰腺炎的诊治

一只8岁雌犬出现食欲不振、精神不佳、呕吐、呼吸急促等症状,采取临床一般检查、犬c反应蛋白检查、ELISA检查后,初诊为犬胰腺炎;后经抗生素滴注后无明显效果,追加血液原虫PCR检查和血涂片检查后确诊为犬吉氏巴贝斯虫感染。根据发病经过及血液学检查,推测该犬是由犬吉氏巴贝斯虫感染继发胰腺炎。经灭虫治疗、对症治疗、支持治疗及输血治疗后,病情得到明显改善。患犬出院后持续口服阿托伐醌配合阿奇霉素,定期复诊结果显示预后良好,最终痊愈。     

一例犬吉氏巴贝斯虫的诊治与临床分析

在国内根据蜱虫的分布,犬巴贝斯虫发病地区主要在南方地区,而北方临床很少见。犬所发生的巴贝斯虫感染通常是由于犬巴贝斯虫和吉氏巴贝斯虫所造成,这两种虫体造成的感染都会导致犬发生渐进性的贫血。吉氏巴贝斯虫感染多是由于蜱叮咬所导致,通常幼犬多见。动物常表现为可视粘膜苍白,心跳过速,呼吸急促,沉郁,食欲不振,虚弱以及血性排泄物。根据感染阶段的不同,有的犬还会出现黄疸,瘀斑现象和肝脏肿大,进而表现出弥漫性血管内凝血(DIC)。发生急性感染的犬,最常出现的症状是严重贫血,弥漫性血管内凝血(DIC),代谢性酸中毒和肾病。慢性长期感染的犬通常会表现出体重减轻和食欲不振的症状。     

一例犬感染吉氏巴贝斯虫的诊治

采用临床检查、血液学检查、影像学检查、巴贝斯虫RT-PCR检测试剂盒等方法对一例9岁的雪纳瑞犬进行检查,根据检查结果最终确诊该犬患有吉氏巴贝斯虫病.患犬临床症状较为严重、血液学检查结果中度贫血,血涂片每个视野可见1～2个虫体,判定病程属于3级,采用第1天、第3天、第7天腿部肌肉注射比利时进口的焦虫针,口服10 d阿奇霉...     

一例犬吉氏巴贝斯虫病的诊断与治疗

文章介绍了1例犬吉氏巴贝斯虫病的诊治情况。通过询问病史、临床检查、血液学检查和聚合酶链式反应（PCR）进行综合分析诊断,采用对症、支持及输血疗法治疗,预后良好。     

一例犬吉氏巴贝斯虫病的PCR诊断

1例拉布拉多病犬,食欲废绝,喜卧好喘,小便呈酱油色,血涂片、姬姆萨染色、镜检发现,红细胞内有淡蓝色的环形虫体。采集病犬血液样品,提取基因组DNA,通过PCR方法及对PCR产物进行限制性片段长度多态性分析进而确诊该病犬为犬吉氏巴贝斯虫感染。     

犬吉氏巴贝斯虫病的诊断与治疗

犬巴贝斯虫病是由硬蜱传播而引起的血源性原虫病,目前本病在宠物犬中经常发生,危害日益严重.该病有一定的发病规律,患犬以严重的贫血和血红蛋白尿为特征.严重感染时可引起贫血、呕吐、腹泻.本文介绍了两个病例,根据病犬的临床症状,通过实验室的检查,诊断出此犬为吉氏巴贝斯虫病,并给予了相应的药物治疗,经治疗后两犬的病情逐渐好转,最...     

36例犬吉氏巴贝斯虫病的诊断与治疗

西安市某宠物医院从2018年-2019年共接诊了36例犬吉氏巴贝斯虫病,对这36例病例的临床症状、流行病学、实验室诊断和治疗进行了调查和研究.结果表明,犬感染吉氏巴贝斯虫后主要症状为虚弱、发热、尿黄等.该病的高发期在9月和10月,占总发病的50％.发病年龄主要是1岁至5岁犬,占69.4％.实验室检查显示感染犬的红细胞和...     

北京地区25例犬吉氏巴贝斯虫病例分析

为调查北京地区犬吉氏巴贝斯虫病的感染与治疗情况,2014年10月至2016年1月期间,本试验对中国农业大学动物医院就诊的25例犬吉氏巴贝斯虫感染病例进行调查统计,记录其临床症状,分析全血细胞计数(Complete Blood Count,CBC)、生化检测结果和三氮脒的治疗效果。结果显示,25例患犬均出现不同程度的贫血,73.3%(11/15)的患犬出现了免疫介导性溶血性贫血(Immune mediated hemolytic anemia,IMHA),使用三氮脒和输血疗法治疗后,71.2%(10/14)的患犬取得良好的疗效,但目前跟踪的病例中,21.4%(3/14)的患犬已复发。     

吉氏巴贝斯虫单克隆抗体制备的研究

应用淋巴细胞杂交瘤技术制备杂交瘤细胞株 ,经间接萤光抗体法筛选和克隆 ,获得了 5株能稳定分泌吉氏巴贝斯虫特异性抗体的杂交瘤细胞株 ,分别命名为C3B5、M8B7、E9C5、G6 D8、H2 A7。经过鉴定 ,这 5株杂交瘤细胞分泌的单克隆抗体亚类及相对分子质量分别为IgG2b,1 8× 1 0 4 ;IgG2a,1 8× 1 0 4 ;IgG1 ,1 8× 1 0 4 ;IgM ,3 2× 1 0 4 ;IgM ,1 8× 1 0 4 。腹水效价为 1∶1 0 4 ～ 1∶1 0 5。其中E9C5杂交瘤细胞株分泌的单克隆抗体是一种保护性抗体 ,经对实验感染吉氏巴贝斯虫小鼠体内虫体的杀虫试验证明具有较强的杀灭作用。     

吉氏巴贝斯虫cDNA探针的制备及杂交试验

本实验将－６．６ｋｂ的吉氏巴贝斯虫ｃＤＮＡ片段以光照活化法标记光敏生物素，制备成光敏生物素探针。与吉氏巴贝斯虫基因组ＤＮＡ、伊氏锥虫基因组ＤＮＡ、犬白细胞ＤＮＡ的斑点杂交试验表明，该探针可与０．００１ｎｇ以上量的吉氏巴贝斯虫ＤＮＡ杂交，而不与任何浓度的伊氏锥虫ＤＮＡ和犬ＤＮＡ杂交，具有很高的敏感性和特异性。     

吉氏巴贝斯虫mRNA的磁性分离及cDNA合成

从感染吉氏巴贝斯虫的摘脾犬的红细胞内提纯虫体,用异硫氰酸胍法提取虫体总RNA,再以Poly(A)~ mRNA磁性分离系统提取mRNA.最后以NotⅠPrimer-Adaptor为引物反转录出cDNA片段.琼脂糖凝胶电泳显示,所合成的cDNA分子量范围由100到6500bP,且绝大部分大于500bP.     

Babesia gibsoni infection in a dog from indiana

Abstract: A 10-year-old spayed female mixed-breed dog was presented to the Purdue University Veterinary Teaching Hospital (PUVTH) with complaints of persistent anemia with occasional exacerbations, anorexia, and lethargy. The dog had been presented to the referring veterinarian 2 months prior with multiple bite wounds received during a fight with 3 Pit Bull Terriers. The dog was discharged after the wounds were cleaned and surgically closed. Upon admission to the PUVTH, blood was collected for a complete blood count and biochemical analysis. Microscopic examination of peripheral blood smears revealed intraerythrocytic protozoal parasites consistent with Babesia gibsoni . Molecular analysis confirmed that the organism was B gibsoni and that its 18S ribosomal RNA sequence was identical to that of other B gibsoni isolates from Oklahoma, North Carolina, and Okinawa, Japan. Hematologic changes included moderately severe, regenerative, macrocytic, normochromic anemia, with poikilocytosis, polychromasia, anisocytosis, and a marked increase in nucleated RBCs. Biochemical changes included increased serum alanine aminotransferase, alkaline phosphatase, and gamma glutamyl-transferase activities. The dog was treated with imidocarb, but despite initial clinical improvement, the dog died 2 weeks after the first dose. A necropsy was not performed. The infection in this dog is the first reported case of B gibsoni infection in Indiana. Because of the widespread geographical distribution of the organism, veterinarians and veterinary clinical pathologists throughout the United States should carefully examine Romanowsky-stained blood smears from patients with acute hemolytic anemia for small intraerythrocytic babesial parasites.     

The Effect of Diminazene Aceturate on Spienectomized Dogs with Babesia gibsoni Infection

The effect of diminazene aceturate on splenectomized and nonspienectomized dogs with Babesia gibsoni (B. gibsoni) infection was investigated. In splenectomized dogs, the fissional and multiplicational stages of B. gibsoni were observed in peripheral blood films, and hemoglobinuria was frequently observed. These findings were different from previous reports and were not changed by administration of diminazene aceturate. It is clear that the intramuscular administration of diminazene aceturate at the dose of 3 mg/kg body weight for 3 days is not effective against B. gibsoni infection in splenectomized dogs.     

Blood, Bull Terriers and Babesiosis: further evidence for direct transmission of Babesia gibsoni in dogs

This study reports on the epidemiology of Babesia gibsoni in American Pit Bull Terriers living in a region of western Victoria in southern Australia. Both American Pit Bull Terriers (n = 100) and other dog breeds (n = 51) were screened for B gibsoni using immunofluorescent antibody testing (IFAT) and/or polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). A questionnaire was also completed by each dog owner, ascertaining the husbandry and habits of the dogs sampled. Fourteen dogs were positive for B gibsoni using IFAT and/or PCR-RFLP and all were American Pit Bull Terriers. Dogs that were male and/or had been bitten by or were biters of other American Pit Bull Terriers were more likely to be B gibsoni positive, thus suggesting that blood-to-blood transmission contributes to the spread of this disease between dogs.     

吉布森巴贝虫顶膜抗原-1基因的表达及表达产物的生物学活性

通过免疫筛选法，从构建的犬吉布森巴贝虫cDNA基因表达文库中筛选到1个cDNA克隆，此cDNA核苷酸全长2108bp，包含1个完整的开放阅读框，编码蛋白的分子质量约为62ku。经过分析，此蛋白（BgAMA-1）与其他巴贝虫属的顶膜抗原-1具有较高的同源性。为了研究其生物学活性，以此cDNA为模板，利用PCR扩增BgAMA-1的全基因，并将其克隆到pGEX-4T-3载体上，构建了原核表达载体pGEX-4T-3／BgAMA-1。通过Western—blotting对表达并纯化的重组蛋白的生物学活性进行分析。结果显示，构建的pGEX-4T-3／BgAMA-1能在大肠杆菌DH5口中表达，所获的重组抗原BgAMA-1在Western—blotting上仅与吉布森巴贝虫感染犬血清发生反应，证实其具有种特异性，在诊断方面可能有一定的应用价值。     

Further epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan during 2015–2018

A single-nucleotide polymorphism causing the replacement of methionine with isoleucine (M121I) in cytochrome b of Babesia gibsoni has been reported to reduce the susceptibility to atovaquone (ATV) in B. gibsoni infection. In our previous study, B. gibsoni with M121I was suggested to exist in nature. Thus, further examinations were performed. In total, 105 genomic DNA samples from B. gibsoni-infected dogs were collected from western (98 samples from 15 prefectures) and eastern areas (7 samples from 4 prefectures) in Japan. The M121I variant population was identified using allele-specific real-time PCR: it was then detected in nine samples (8.57%), which was higher than that in the previous study (4.11%). Although there are unclear points, such as the history of ATV usage, careful attention should be given to emerging ATV resistance.