工厂化荞麦芽苗生产技术

荞麦芽苗又称芦丁,苦荞麦苗,不仅有食用价值,而且具有保健功能,是市场上深受欢迎的芽苗蔬菜。荞麦芽苗的生产方法很多,可以用育苗盘进行立体栽培,基质有珍珠岩、蛭石,也可以铺报纸。如用育苗盘生产荞麦芽苗,每盘播种量为200g左右,芽苗产量在1.6kg左右,生产周期为8～10天。荞麦芽苗也可采用土培法和沙培法生产。每平方米播种量为800g左右,产量在4kg左右。以应用育苗盘进行荞麦芽苗生产比较普遍。其技术要点如下:1.生产场地和设施用育苗盘进行荞麦芽苗生产多在遮阳网的温室或大棚中栽培,可以多层立体栽培,也可只种一层。通常使用的育苗盘为平…     

基于自制水培装置培养的花生芽苗白藜芦醇含量分析

研究花生芽不同培养方式、不同培养天数、不同组织部位白藜芦醇含量的差异及其进一步培养成花生苗白藜芦醇含量的变化。运用水培和土培两种方式进行花生发芽,利用高效液相色谱仪(HPLC)检测分析不同发芽天数花生芽白藜芦醇含量,选出最优发芽时间,再利用自制水培装置将花生芽培养成花生苗,对其根、茎、叶白藜芦醇含量进行分析。结果表明:花生芽发芽后5 d,其白藜芦醇含量最高,土培和水培含量分别为117.6、42.27μg/g。自制水培装置培养的花生苗根系发达,其白藜芦醇含量明显高于花生芽含量,花生苗根部白藜芦醇的含量达143.4μg/g,远高于茎、叶的含量。装置制备成本为350.0元,制作材料简单,使用操作简单,可种植各种蔬菜及花卉,改变了传统果蔬主要由大田种植的格局。     

浅水藕组织培养苗培育成功

江苏省响水县浅水藕科技示范园区通过用优质藕--鄂莲四号精心培育的"组织苗"最近获得了成功. 该县从扬州大学农学院(邮码:225001)引进技术,合作从事浅水藕"组织培养苗"的试培、试栽."组织培养苗"是一种繁育不用种,传代靠"克隆"的无性繁殖苗,它只需选取优质成熟藕上的一个芽,就可培育出2000多棵幼苗,且只需两个月就可移到大田内栽植.省去了以往667平方米地需留下600多斤的成藕,用作下一茬种子的成本和时间.(照成藕1.2元/公斤算,传统藕种需300多元,而用组织培养苗作种,只需50元;传统栽种667平方米藕需劳动力4个,而现在只需1个.)而且由于它是无菌培育,且一生不用施肥,是真正的无公害绿色食品.     

甜樱桃砧苗繁育有五招

<正>1组培快繁试管苗培养将经过灭菌处理的种胚、茎尖等外植体经过灭菌处理后接种在萌发与生长培养基上,2周左右,茎尖萌动,20天芽迅速增大,30天后分化出丛芽,45天以后,长高到2厘米左右,成为带有4～5片叶的试管     

马铃薯夏播掰苗留种试验

马铃薯夏播掰苗留种能够恢复种性,延长品种使用寿命,是防止退化的一条经济简便的实用技术,解决了农民自留种问题.     

不同品种甘薯脱毒苗试验初报

甘薯为无性繁殖作物,在育种栽培中极易感染病毒而引起种性退化、产量降低、品质变劣,甚至完全丧失商品价值.甘薯脱毒栽培是克服种性退化的有效措施,但脱毒效果有随品种不同而异的表现.本试验旨在探讨不同品种甘薯脱毒苗的产量和农艺性状表现.     

香椿苗

用香椿籽培育出来的香椿苗长相跟香椿树芽大不一样,但味道差不多。香椿树芽生长在户外,一年内可食用的时间也就在4、5月份,庭院里栽培的香椿苗可不一样,啥时想吃啥时种,顶多半个月,就能收获了。产量也十分悬殊,667m2香椿树一年的产芽量只有150kg左右,而采用立体栽培的香椿苗,十     

桃树芽苗栽培技术

为了进一步推动芽苗栽培在生产上的应用,根据我们1973～1980年的试验结果和取得的基本经验加以概括性的总结,供参考。一、桃树芽苗栽培的意义桃树的芽苗,在过去被叫做“半成苗”。它的意思是夏秋用芽接的方法在毛桃砧木上接上一个栽培品种的芽子,不等其次年萌发,即在当年的冬天或次年的春天出圃定植,因为这种苗木只有一个栽培品种的“接芽”,故称“芽苗”。称它为芽苗的意思还有两个:一个是     

脱毒马铃薯试管苗高海拔网棚移栽死苗的预防措施

马铃薯脱毒培养已成为保持马铃薯优良种性的一项有效技术,也是解决种性退化的十分有效的方法,该项技术已得到普遍应用。乐都县马铃薯种植面积大,它已成为乐都县经济发展的支柱产业,所以需种量很大。为满足广大农民的需求,并保持品种的     

基于表型性状的铁观音茶树种性变化分析

为研究我国重要茶树品种铁观音的种性变化,在原产地福建省安溪县选取了80份铁观音茶树为研究对象,对其主要表型性状进行了观测分析.结果表明:供试茶树在树姿、芽叶颜色和侧脉对数等性状上差异较大,变异系数分别为48.95%、36.83%和21.77%;主成分分析表明,叶形、芽叶色泽和树姿3项因子可以作为判别铁观音茶树性状变化的重要参考指标;基于表型性状的聚类分析可以将80份铁观音茶树分为4个类群.由此可以推测,铁观音茶树品种存在种性退化现象.     

浅析实施人才战略中人才培养选拔和使用二者的辩证关系

指出在当前竞争日趋激烈的国际环境下，要把培养、吸引人才放在实施人才战略首位；提出要用两点论原则、任人唯贤原则、德才兼备原则选拔人才；阐明培养、选拔人才的目的在于应用，而要用好人才，充分调动其积极性与创造性，就必须遵循用其所长、适才所用、用养并重的用人原则。     

表皮干细胞的分离、纯化培养及鉴定初报

采用酶消化法和组织块法对小鼠、奶山羊、奶牛和家猪的表皮干细胞进行了分离、培养。结果表明:酶消化法适于小鼠表皮干细胞的分离,所获得的小鼠表皮干细胞在维持了两代之后自发分化为神经样细胞;奶山羊和奶牛不论用酶消化法还是组织块法都可以获得大量活力相对较好、表皮干细胞特性维持较久的细胞,奶山羊表皮细胞采用型胶原分选,用有血清培养液培养,经免疫细胞化学鉴定为表皮干细胞,可持续传至少9代,随着代数增高,细胞活力减弱。     

植物组织培养中降低培养基成本的研究

在马铃薯、菊花和满天星的发根阶段研究了用自来水代替蒸馏水、白糖代替蔗糖的可行性．结果表明：马铃薯、菊花和满天星培养苗的生长在蔗糖与白糖、蒸馏水与自来水的处理间无显著性差异，因此规模化生产上配制培养基时用白糖取代蔗糖，自来水取代蒸馏水完全可行．     

Cryopreservation, culture, and transplantation of human fetal mesencephalic tissue into monkeys

Studies in animals suggest that fetal neural grafts might restore lost neurological function in Parkinson's disease. In monkeys, such grafts survive for many months and reverse signs of parkinsonism, without attendant graft rejection. The successful and reliable application of a similar transplantation procedure to human patients, however, will require neural tissue obtained from human fetal cadavers, with demonstrated cellular identity, viability, and biological safety. In this report, human fetal neural tissue was successfully grafted into the brains of monkeys. Neural tissue was collected from human fetal cadavers after 9 to 12 weeks of gestation and cryopreserved in liquid nitrogen. Viability after up to 2 months of storage was demonstrated by cell culture and by transplantation into monkeys. Cryopreservation and storage of human fetal neural tissue would allow formation of a tissue bank. The stored cells could then be specifically tested to assure their cellular identity, viability, and bacteriological and virological safety before clinical use. The capacity to collect and maintain viable human fetal neural tissue would also facilitate research efforts to understand the development and function of the human brain and provide opportunities to study neurological diseases.     

SD大鼠睾丸支持细胞的分离、培养、纯化及鉴定

为进一步简化、优化SD大鼠睾丸支持细胞的体外培养条件及鉴定方法,采用三酶两步消化法和差异贴壁法分离、培养、纯化SD大鼠支持细胞,并用HE染色、油红染色、Feulgen染色及透射电镜扫描其超微结构予以鉴定。结果表明：平均0．10g睾丸组织可获得2．5×10^6个支持细胞,细胞存活率90％以上,体外培养5h后开始贴壁,96h后细胞生长速率下降,其对数生长期为3—7d,整个体外生长周期约为10～15d。对获得的纯化细胞进行鉴定,发现其形态结构及超微结构与支持细胞的形态特征一致。可见,采用三酶两步消化法和差异贴壁法,可达到分离、培养、纯化SD大鼠支持细胞的目的。     

Political economic history,culture, and Wounaan livelihood diversity in eastern Panama

A growing literature on scholarly and practical approaches to conservation and development uses a livelihood approach to understand rural peoples’ diverse assets and activities, especially as they serve to minimize vulnerability to economic and ecological shocks. In recent years, the suite of potential assets available to rural households has been theorized as human, natural, physical, social, and cultural capitals and includes the context in which they are used. Here we explore Wounaan livelihood strategies and how they articulate with the dynamic political economic history of eastern Panama. Known in Panama as forest dependent swiddeners, semi-structured interviews and participant observation revealed Wounaan’s increasing reliance on fishing, artisanship, and ecotourism in their income profiles. While these income sources are linked to decreasing land availability and increasing market opportunities, we address the role of cultural beliefs and values in Wounaan negotiation of their income strategies. J. Vela′squez Runk has studied ecology and anthropology in eastern Panama for ten years. Presently she is a Resident Scholar at the School of American Research in Santa Fe, New Mexico. Gervacio Ortíz is a business student at the University of Panama and a resident of Majé, Panama. Wilio Quintero is a resident of Boca Lara, Panama. Cristobalino Quiróz is a resident of Boca Lara, Panama.