Spatial patterns of leprosis and its mite vector in commercial citrus groves in Brazil

In order to describe the dissemination of Citrus leprosis virus (CiLV) and Brevipalpus phoenicis , the Tenuipalpidae mite which transmits it, as well as to generate data for future development of better sampling and disease control procedures, analyses were conducted at three levels of spatial hierarchy. Over 100 distribution maps of mite-infested plants and plants with CiLV symptoms were constructed after evaluation of 174 sweet orange commercial grove blocks from four citrus regions of São Paulo State, Brazil. Spatial correlation between maps of mite-infested plants and those with CiLV symptoms was very low and mostly not significant. Spatial dependency between adjacent plants was incipient as ordinary runs analysis showed that percentages of aggregated sequences within or across rows were very low for both mite-infested and diseased plants. Index of dispersion ( D ) values for all quadrat sizes suggested aggregation of plants with symptoms within quadrats, but much less aggregation for mite-infested plants. Values of log( A ) and b were higher than 0 and 1, respectively, indicating a general and significant aggregation of infested/diseased plants inside quadrats. However, the degree of aggregation of plants with symptoms was higher than that of infested plants. Aggregation in each grove was positively correlated to the incidence of infested/diseased plants. Spatial autocorrelation also showed a higher strength of aggregation for plants with symptoms than for infested ones. This is the first time that spatial patterns of leprosis and B. phoenicis have been described.     

Influence of Bacillus spp. isolated from maize agroecosystem on growth and aflatoxin B(1) production by Aspergillus section Flavi

A total of 59 bacteria of the Bacillus genus were isolated from different components of a maize agroecosystem and their antifungal activity against Aspergillus section Flavi was evaluated. Thirty-three and 46% of these bacteria were able to inhibit Aspergillus flavus Link and A. parasiticus Speare respectively at water activity (a(w)) 0.982; however, when a(w) was 0.955, these percentages were decreased and only three isolates were able to inhibit Aspergillus section Flavi. The majority of bacilli acted as contact antagonists, while a small number of isolates were able to form inhibition zones. In maize meal extract agar, Aspergillus section Flavi growth rate and aflatoxin B(1) (AFB(1)) production were significantly reduced when these strains were paired at a(w) 0.982 with bacilli at all inoculum levels studied. However, two bacilli isolated were able to reduce growth rate and aflatoxin production when a(w) was 0.955. Lag phase increase followed the same general pattern as growth rate reduction. When Aspergillus section Flavi was grown in sterile maize in the presence of three Bacillus strains at a(w) 0.982, the reduction in count (colony-forming units (cfu) g(-1) maize) was less than 30%, except when Aspergillus section Flavi grew with Bacillus amyloliquefaciens UNRCLR. However, levels of detectable AFB(1) were significantly reduced in these interactions at a(w) 0.982.     

猪毛蒿精油及其活性成分萜品-4-醇对黄曲霉的抑制作用研究

为了探究猪毛蒿Artemisia scoparia精油及其活性成分萜品—4—醇对黄曲霉Aspergillus flavus的抗菌活性及其在花生贮藏保鲜中的应用前景,通过气相色谱—质谱联用（Gas Chromatography—Mass Spectrometer, GC—MS）技术对猪毛蒿精油进行成分分析;采取熏蒸法检测猪毛蒿精油及萜品—4—醇对黄曲霉菌丝生长,孢子萌发和花生体内霉菌数的影响;通过扫描电镜观察猪毛蒿精油和萜品—4—醇对黄曲霉显微结构的影响。结果显示猪毛蒿精油由24种化合物组成,其主要成分为桉叶油醇（43.8%）,萜品—4—醇在猪毛蒿精油中的含量为2.81%;猪毛蒿精油和萜品—4—醇可显著抑制黄曲霉菌丝的生长,并导致菌丝皱缩、扭曲和凹陷;体内抑菌试验发现,猪毛蒿精油和萜品—4—醇能显著降低花生体内霉菌数。     

塔里木河下游阿拉干断面胡杨林空间分布特征及其影响因素

通过小尺度生态监测与遥感数据分析获取塔里木河下游荒漠河岸林现状信息,集中对阿拉干断面设立的100hm2长期监测样方内的胡杨树进行了详细的调查,对该断面胡杨林空间分布特征及其影响因素进行了分析。研究结果表明：塔里木河下游的胡杨具有伴河生长的特性;随着离河距离的增加,胡杨株数呈递减趋势;大部分胡杨主要分布在离河道200m范...     

遥感和GIS支持下县域退耕还林还草空间决策分析--以呼和浩特市武川县为例

退耕还林还草是当前生态环境建设和保护的重要途径 ,在遥感和GIS技术支持下将武川县的土壤类型数据与地形坡度数据叠加分析同时参考土壤侵蚀、降水量和温度等方面的资料 ,进行土地适宜性评价 ,对TM影像目视解译得到土地利用现状数据 ,并与土地适宜性数据进行叠加分析 ,确定退耕还林还草地的数量及其空间分布。可为决策部门提供决策依据。此研究方法和技术路线作为一种技术模式 ,可进行推广 ,对西部大开发中退耕还林还草工程项目的顺利进行有重要意义[1 ] 。     

<Emphasis Type="Italic">Spongospora subterranea</Emphasis> soil contamination and its relationship to severity of powdery scab on potatoes

We investigated soil contamination by Spongospora subterranea f. sp. subterranea (Sss) and disease severity of powdery scab in 29 potato fields in Hokkaido, Japan, using a hydroponic culture method with tomato seedlings as bait plants. The quantity of Sss infection on the roots of bait plants was evaluated using the polymerase chain reaction (PCR) and expressed in terms of the infection potential in the soil. The infection potential was positively correlated with the disease severity of harvested tubers, whereas the spore ball density determined using PCR had an indistinct relationship with disease severity. The infection potential can be useful in evaluating soil contamination and in applying countermeasures against powdery scab.     

Spatiotemporal variation in vegetation net primary productivity and its relationship with meteorological factors in the Tarim River Basin of China from 2001 to 2020 based on the Google Earth Engine

Vegetation growth status is an important indicator of ecological security. The Tarim River Basin is located in the inland arid region of Northwest China and has a highly fragile ecological environment. Assessing the vegetation net primary productivity (NPP) of the Tarim River Basin can provide insights into the vegetation growth variations in the region. Therefore, based on the Google Earth Engine (GEE) cloud platform, we studied the spatiotemporal variation of vegetation NPP in the Tarim River Basin (except for the eastern Gobi and Kumutag deserts) from 2001 to 2020 and analyzed the correlations between vegetation NPP and meteorological factors (air temperature and precipitation) using the Sen slope estimation method, coefficient of variation, and rescaled range analysis method. In terms of temporal characteristics, vegetation NPP in the Tarim River Basin showed an overall fluctuating upward trend from 2001 to 2020, with the smallest value of 118.99 g C/(m²?a) in 2001 and the largest value of 155.07 g C/(m²?a) in 2017. Regarding the spatial characteristics, vegetation NPP in the Tarim River Basin showed a downward trend from northwest to southeast along the outer edge of the study area. The annual average value of vegetation NPP was 133.35 g C/(m²?a), and the area with annual average vegetation NPP values greater than 100.00 g C/(m²?a) was 82,638.75 km², accounting for 57.76% of the basin. The future trend of vegetation NPP was dominated by anti-continuity characteristic; the percentage of the area with anti-continuity characteristic was 63.57%. The area with a significant positive correlation between vegetation NPP and air temperature accounted for 53.74% of the regions that passed the significance test, while the area with a significant positive correlation between vegetation NPP and precipitation occupied 98.68% of the regions that passed the significance test. Hence, the effect of precipitation on vegetation NPP was greater than that of air temperature. The results of this study improve the understanding on the spatiotemporal variation of vegetation NPP in the Tarim River Basin and the impact of meteorological factors on vegetation NPP.