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本文介绍了平皿抑菌计数法、最低抑菌浓度法、杯碟法三种检测防霉剂防霉效果的方法,并选择丙酸钙和双丙酸铵作为实验材料,通过将其添加到培养基中来抑制米曲霉的生长。通过最低抑菌浓度法的实验,我们测定了丙酸钙和双丙酸铵对米曲霉的最低抑菌浓度,此外,通过将最低抑菌浓度法与平皿抑菌计数法、杯碟法的实验结果相比较,结果我们发现这三种方法在测定丙酸钙和双丙酸铵对米曲霉的抑制效果上,结果是一致的。本文中介绍的三种方法,实验周期短,可用于防霉剂样品的初步筛选。 相似文献
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饲料防霉剂防霉效果的检测方法——平皿计数法 总被引:4,自引:0,他引:4
盲目挑选和使用饲料防霉剂,往往不仅事与愿违,更会危害饲料企业的经济利益及品牌声誉。所以,掌握并采用科学、正确、有效的检测方法,自我判别防霉剂产品之优劣是各饲料企业维护自身利益的当务之急。 目前用于饲料防霉剂防霉效果的检测对比之方法有:杯碟法、最低抑菌浓度法、二氧化碳释放法、温度测定法、饲料常规试验、高温高湿法等。杯碟法测定最大抑菌圈直径,用其大小来定量测定其防霉效果,试验要求选配一种理想溶剂,要求较高、操作复杂;最低抑菌浓度法需将各种防霉剂配成浓度梯度液,工作量大,与杯碟法一样仅适于作初选、… 相似文献
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房兴堂 《养殖与饲料.饲料世界》2006,(5):22-24
本文在分析饲料霉菌危害原因、现状和饲料防霉剂使用、饲料防霉技术基础上介绍杯碟法、最低抑菌浓度法、饲料强化防霉、平皿记数法等防霉剂防霉效果的检测方法和溶菌酶、鱼精蛋白、聚赖氨酸、纳他霉素、大蒜素、杜仲提取物等天然防霉剂开发应用情况。 相似文献
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饲料霉变及霉菌毒素污染严重影响着饲料工业和畜牧业生产,因此,国内外的饲料生产厂商和科研人员,十分重视防霉技术的研究和应用。近年来,采用的饲料防霉技术主要有以下几种:辐射灭菌;添加防霉剂;使用防霉包装袋;化学消毒和辐射结合防霉;控制真菌遗传密码。目前,主要在饲料中添加防霉剂,市场上防霉剂种类繁多,适用范围及防霉效果不尽相同,如使用不当还会引起中毒现象,如何选用合适的防霉剂是在实际生产中值得重视的问题。人们在使用防霉剂时往往注重防霉剂的价格,而忽视防霉剂防霉效果。为了解防霉剂防霉效果,更好地合理使用防霉剂。本文介绍影响防霉剂作用效果的因素和防霉剂防霉效果的检测方法,供同行参考。 相似文献
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饲料防霉剂效果检测与天然防霉剂开发 总被引:1,自引:0,他引:1
介绍了杯碟法、最低抑菌浓度法、饲料强化防霉、平皿记数法等防霉剂防霉效果的检测方法和溶菌酶、鱼精蛋白、聚赖氨酸、纳他霉素、大蒜素、杜仲提取物等天然防霉剂的开发应用情况。 相似文献
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影响防霉剂效力及饲料防霉效果的因素 总被引:5,自引:0,他引:5
前言我国南方地区春、夏季节高温高湿,饲料防霉保质是一件重要的工作。常见的饲料霉菌包括根霉、毛霉、曲霉、青霉、木霉、镰刀霉属等真菌。饲料霉变的危害有:饲料结块、产生异味引起畜禽拒食、营养成份损失、消化率降低、霉菌毒素(如黄曲霉素等)引起畜禽中毒甚至死亡... 相似文献
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饲料霉变是一个非常普通的现象,防止饲料霉变也是重点研究之一。通过添加30%、25%、20%、15%、0%丙酸含量的防霉剂分别设为A、B、C、D和E组,试验期50天,前20天测量饲料温度的变化,试验全期观察饲料霉变情况并进行霉菌计数。结果表明:前20天饲料温度变化曲线相似,差异不显著(P>0.05);随着饲料中丙酸含量的降低,防霉效果逐渐变差,试验A组防霉效果最好,试验D组效果最差,A组与D组比较差异显著(P<0.05);从霉菌的生长情况来看,不添加防霉剂的生长速度最快最多,试验A组与对照组差异极显著(P<0.01),试验B组和试验C组与对照组差异显著(P<0.05),试验D组与对照组差异不显著(P>0.05),试验A组与试验D组差异显著(P<0.05),其它各试验组间差异不显著(P>0.05)。饲料中丙酸含量高的防霉剂其防霉效果要好。 相似文献
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ZHU Yu-fei XIE Shu-yu CHEN Dong-mei WANG Yu-lian PAN Yuan-hu PENG Da-peng TAO Yan-fei YUAN Zong-hui 《中国畜牧兽医》2016,43(7):1785-1791
With the development of animal husbandry and the widespread use of feed additives,the indiscriminate use and abuse of feed additives had become a problem to be settled.In the study,the detection techniques of feed additives and the shortages of feed additive detection technology at present were summarized.At the same time,the development trend of feed additives for high-throughput screening technology was described,aiming to apply the technical supports for the green and safe production. 相似文献
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三种测定饲料水分含量的方法比较 总被引:1,自引:0,他引:1
通过国标法(GB6435-86,简称GB法)、国标改进法(简称改进法)和Sh10A水分快速测定仪法(简称仪器法)三种方法测定饲料中水分含量,并对试验数据进行数理统计分析,结果表明:三种方法无显著性差异。 相似文献
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This study compares three fluorescence methods for the evaluation of boar sperm viability and examines the interrelationships between fluorescence viability and motility of liquid semen, stored for 7 days, and fertility. One artificial insemination dose from each of 102 boars was stored for 7 days at 20°C. Plasma membrane integrity was evaluated in three different ways. First, a combination of two fluorophore probes, calcein acetylmethyl ester and ethidium homodimer-1 (EH), was used to stain samples for fluorescence microscopic analysis by making semen smears. Secondly, semen samples were stained with EH with and without the addition of a detergent (ATP-releasing reagent), and fluorescence intensities were measured with a fluorometer. Thirdly, fluorometric evaluation was carried out in the same pattern as with EH, by means of a more permeable, less specific DNA fluorochrome (more interference with RNA), Hoechst 33258. Computer-assisted motility assessment gave the values for total, rapid and progressive motility and path velocity in semen stored for 7 days. Fertility of the boars was determined by the nonreturn (NR%) rate within 60 days of first inseminations and litter size of multiparous farrowings. The results showed that all three fluorescence methods were strongly intercorrelated. All plasma membrane integrity parameters correlated significantly with motility parameters and with fertility parameters (NR% and litter size of multiparous farrowings), but motility parameters did not correlate with NR%. It seems that fluorometric measurement could prove useful for plasma membrane integrity studies in liquid boar semen. Use of the objective and fast fluorometer-based viability assay is thus suitable for several applications in sperm studies. 相似文献
This study compares three fluorescence methods for the evaluation of boar sperm viability and examines the interrelationships between fluorescence viability and motility of liquid semen, stored for 7 days, and fertility. One artificial insemination dose from each of 102 boars was stored for 7 days at 20°C. Plasma membrane integrity was evaluated in three different ways. First, a combination of two fluorophore probes, calcein acetylmethyl ester and ethidium homodimer-1 (EH), was used to stain samples for fluorescence microscopic analysis by making semen smears. Secondly, semen samples were stained with EH with and without the addition of a detergent (ATP-releasing reagent), and fluorescence intensities were measured with a fluorometer. Thirdly, fluorometric evaluation was carried out in the same pattern as with EH, by means of a more permeable, less specific DNA fluorochrome (more interference with RNA), Hoechst 33258. Computer-assisted motility assessment gave the values for total, rapid and progressive motility and path velocity in semen stored for 7 days. Fertility of the boars was determined by the nonreturn (NR%) rate within 60 days of first inseminations and litter size of multiparous farrowings. The results showed that all three fluorescence methods were strongly intercorrelated. All plasma membrane integrity parameters correlated significantly with motility parameters and with fertility parameters (NR% and litter size of multiparous farrowings), but motility parameters did not correlate with NR%. It seems that fluorometric measurement could prove useful for plasma membrane integrity studies in liquid boar semen. Use of the objective and fast fluorometer-based viability assay is thus suitable for several applications in sperm studies. 相似文献
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饲料中种钙含量测定方法的比较研究 《畜牧与饲料科学》2016,37(10):38-38
试验采用高锰酸钾法(GB/T 6436-2002)、乙二胺四乙酸二钠络合滴定法(EDTA法,GB/T 6436-2002)和均相沉淀法对11种饲料原料进行钙含量测定的比较研究。结果表明,对饲料中的钙含量进行测定,3种方法所获得的结果差异均不显著(P〉0.05)。其中,高锰酸钾法准确度高于EDTA法和均相沉淀法,但过程复杂、时间长;EDTA法简单、快速,但测定结果不好判断;均相沉淀法比较生僻,很少在实际生产中应用。 相似文献
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食品及饲料中三聚氰胺检测方法的比较 《畜牧与饲料科学》2015,36(4):97-97
介绍了国内外近年来对三聚氰胺定量检测的方法。主要涉及奶粉等乳制品及饲料中三聚氰胺的检测,包括色谱法、色谱-质谱联用法(GC—MS、LC—MS)、光谱法及免疫法等,通过分析和比较可以看出,上述典型的检测方法检测费用较高,仅限于实验室检测,难于推广,或者虽然检测费用低,但检测结果不准确,而纳米金、石墨烯等新型材料的引入,对微量的三聚氰胺检测能够更加快速、便捷、准确。 相似文献
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急性肝胰腺坏死综合征(acute hepatopancreas necrosis disease,AHPND)是一种危害大、新出现的对虾疫病。目前认为该病由一种副溶血性弧菌(Vibrio parahaemolyticus,VP)引起。本试验通过生化鉴定、血清学分型、MALDI-TOF-MS鉴定以及文献建立的PCR方法,在福建省南美白对虾养殖场,对分离于AHPND病虾肝胰腺的VP进行了检测。通过VITEK-2鉴定出81株VP分离株。从中选取9株分别进行MALDI-TOF-MS鉴定、PCR检测和血清学分型,发现这9株分离菌株均与MALDI-TOF-MS数据库中的VP匹配。其中:4株PCR检测为阳性,被鉴定为AHPND VP,其血清型包括O1:KUT和O1:K68;另5株PCR检测为阴性,血清型包括O1:KUT、O3:K6和O1:K68。试验表明:AHPND VP分离株存在不同血清型,可通过MALDI-TOF-MS进行菌种鉴定;MALDI-TOF-MS与PCR结合使用,可以准确、快速鉴定AHPND VP,这有利于开展AHPND的病原学分析、流行病学调查以及诊断和监测。 相似文献
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尼卡巴嗪(nicarbazin,NIC)是一类由4,4’-二硝基苯脲(4,4-dinitrocarbanilide,DNC)和2-羟基-4,6-二甲基嘧啶(2-hydroxyl-4,6-dimethylpyrimidine,HDP)组成的等分子复合物,其中DNC为其残留标志物。NIC因安全高效被广泛应用于鸡和火鸡等禽类球虫病防治。近年来,NIC残留问题高发,严重威胁动物源性食品安全和消费者生命健康安全。目前,针对禽类产品和饲料中NIC分析方法主要包括仪器分析方法和免疫分析方法两大类。其中,仪器分析方法作为确证方法,具有高特异性、高灵敏度、结果可靠的优点,主要包括高效液相色谱法(high performance liquid chromatography,HPLC)和液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)等。免疫分析方法是NIC快速检测方法的主流技术,具有速度快、成本低、实用性强的优点,主要包括酶联免疫检测法(enzyme-linked immunosorbent assay,ELISA)、免疫层析方法(immunochromatography,IC)、表面等离子体共振(surface plasmon resonance,SPR)、荧光免疫检测法(fluorescent immunoassay,FIA)和流式细胞术(flow cytometry,FCM)等。笔者综述了近40年来国内外禽类产品和饲料中NIC的检测分析方法,以期为NIC残留监控提供理论和实践指导依据。 相似文献
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不同方法检测奶牛结核病的结果分析 总被引:1,自引:0,他引:1
采用常规皮内变态反应(TST)、牛全血干扰素试验方法(IFN-γ)及胶体金3种检测方法,对2个奶牛场的600头奶牛进行结核病检测,发现结核病阳性率均偏高,表明奶牛场结核病感染较严重。将3种方法检测结果的敏感性、特异性及符合率进行比较,结果表明:单独使用PPD皮内变态反应方法或胶体金方法在牛结核病的诊断方面存在特异性及符合率差的缺点;用IFN-γ实验进行检测,可以提高特异性,避免因假阳性造成的误杀,适合在本地区推广应用。 相似文献