共查询到20条相似文献,搜索用时 31 毫秒
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María Teresa Tejedor-Junco Margarita González-Martín Estefanía Bermeo-Garrido Rebeca Villasana-Loaiza Elena Carretón-Gómez 《Veterinary research communications》2018,42(3):227-232
Doxycycline is an antibiotic that, in addition to the classic antibacterial use, is also prescribed to fight parasitic diseases, like heartworm disease in dogs. Despite the concern that the overuse of this antibiotic may decrease susceptibility of clinically important bacteria, the consequences of the prolonged doxycycline therapy in heartworm-infected dogs have never been studied before. We have analyzed the impact of this therapy on Staphylococcus aureus and Enterococcus antimicrobial resistance. In this study, 17 heartworm-infected dogs (10 that had completed the doxycycline treatment and 7 dogs that had not yet begun) were included. Twenty-four isolates of Staphylococcus aureus were obtained from two locations of each dog. After treatment, 73.3% of isolates were resistant to at least one antibiotic but only 22.2% of isolates before treatment. Most of doxycycline resistant isolates were obtained from dogs that have received treatment. Erythromycin resistance or intermediate susceptibility was detected in 45.6% of isolates, most of them from dogs after treatment. For Enterococci, 48 isolates were obtained from fecal samples (25 before treatment and 23 after treatment). Before treatment, 32% of isolates were resistant at least to one antibiotic while after, this data increase up to 65%. Comparing isolates before and after treatment, a clear increase in resistance to doxycycline (12% against 21.74%) and erythromycin (20% against 39.13%) was observed. Although the present work is a preliminary research, the results encourages the development of further studies to determinate the effect of prolonged doxycycline therapy on antimicrobial resistance. 相似文献
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S. Heidari Abdollah Derakhshandeh R. Firouzi M. Ansari-Lari M. Masoudian V. Eraghi 《Tropical animal health and production》2018,50(4):779-785
Abortion in sheep and goats has become increasingly important worldwide because of the significant economic losses and potential zoonotic implication of commonly involved pathogens. Therefore, this cross-sectional study was conducted in southern Iran to detect the Chlamydophila abortus and Coxiella burnetii, as zoonotic pathogens, and Mycoplasma agalactiae, as a neglected abortifacient agent in small ruminants’ aborted fetuses, by using polymerase chain reaction (PCR). From a total of 300 aborted fetuses (183 sheep and 117 goats), 46 samples (15.5%) were positive by PCR, 11% for C. abortus, 2% for C. burnetii, and 3% for M. agalactiae. Also, the association of suggested risk factors with abortion due to these bacterial agents was investigated using univariable and multivariable logistic regression. Results of the statistical analysis showed significant association of C. abortus with flock size (OR?=?2.82, P?=?0.014), season (P?<?0.05), and the number of pregnancy in the aborted dam (OR?=?2.5, P?=?0.05). Our results indicated that C. abortus has a relatively substantial role in small ruminant abortions, and C. burnetii and M. agalactiae are likely important abortifacient agents in our region, too. Regarding veterinary and/or public health importance of these bacterial agents, more attention from veterinary and/or human health services and, maybe, a surveillance system for control and prevention of them are recommended. 相似文献
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I Made Damriyasa Gereon Schares Christian Bauer 《Tropical animal health and production》2010,42(1):95-98
A cross-sectional survey was performed to obtain first information on the presence of Neospora caninum infection in Bos javanicus (‘Bali cattle’), the predominant beef cattle in the Eastern Islands of Indonesia. Serum samples were collected from 438 Bali
cattle of two age classes (<2 years, >2 years) and both genders at three slaughterhouses in the Bali island, and examined
for N. caninum-specific antibodies using native NcSRS2 (p38 antigen) as an ELISA antigen. The estimated overall seroprevalence of antibodies
was 5.5% (95% CI: 3.5–8.0%). The seroprevalence was not significantly associated with age class or gender of the animals.
The results give first serological evidence for the presence of natural N. caninum infection in Bos javanicus and indicate its occurrence in Indonesia. 相似文献
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Manju Singh Shoor Vir Singh Saurabh Gupta Kundan Kumar Chaubey Bjorn John Stephan Jagdip Singh Sohal Manali Dutta 《Veterinary research communications》2018,42(3):183-194
Early rapid detection of Mycobacterium avium subspecies paratuberculosis (MAP) bacilli in milk samples is the major challenge since traditional culture method is time consuming and laboratory dependent. We report a simple, sensitive and specific nano-technology based ‘Nano-immuno test’ capable of detecting viable MAP bacilli in the milk samples within 10 h. Viable MAP bacilli were captured by MAP specific antibody-conjugated magnetic nano-particles using resazurin dye as chromogen. Test was optimized using true culture positive (10-bovine and 12-goats) and true culture negative (16-bovine and 25-goats) raw milk samples. Domestic livestock species in India are endemically infected with MAP. After successful optimization, sensitivity and specificity of the ‘nano-immuno test’ in goats with respect to milk culture was 91.7% and 96.0%, respectively. Whereas, it was 90.0% (sensitivity) and 92.6% (specificity) with respect to IS900 PCR. In bovine milk samples, sensitivity and specificity of ‘nano-immuno test’ with respect to milk culture was 90.0% and 93.7%, respectively. However, with respect to IS900 PCR, the sensitivity and specificity was 88.9% and 94.1%, respectively. Test was validated with field raw milk samples (goats-258 and bovine-138) collected from domestic livestock species to detect live/viable MAP bacilli. Of 138 bovine raw milk samples screened by six diagnostic tests, 81 (58.7%) milk samples were positive for MAP infection in one or more than one diagnostic tests. Of 81 (58.7%) positive bovine raw milk samples, only 24 (17.4%) samples were detected positive for the presence of viable MAP bacilli. Of 258 goats raw milk samples screened by six diagnostic tests, 141 (54.6%) were positive for MAP infection in one or more than one test. Of 141 (54.6%) positive raw milk samples from goats, only 48 (34.0%) were detected positive for live MAP bacilli. Simplicity and efficiency of this novel ‘nano-immuno test’ makes it suitable for wide-scale screening of milk samples in the field. Standardization, validation and re-usability of functionalized nano-particles and the test was successfully achieved in field samples. Test was highly specific, simple to perform and easy to read by naked eyes and does not require laboratory support in the performance of test. Test has potential to be used as screening test to estimate bio-load of MAP in milk samples at National level. 相似文献
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Hellemans A Chiers K Decostere A De Bock M Haesebrouck F Ducatelle R 《Veterinary research communications》2007,31(4):385-395
‘Candidatus Helicobacter suis’ is a spiral-shaped bacterium that colonizes the stomach of more than 60% of slaughter pigs. The role of
‘Candidatus Helicobacter suis’ in gastric disease of pigs is still unclear. Experimental studies in pigs are lacking because this bacterium
is unculturable until now. An inoculation protocol using ‘Candidatus Helicobacter suis’ infected mouse stomach homogenate was used to reproduce the infection in pigs. Control animals were inoculated
using negative mouse stomach homogenate. Pigs were inoculated three times with one-week intervals and euthanized 6 weeks post
inoculation. Tissue samples were taken from different mucosal stomach regions to detect ‘Candidatus Helicobacter suis’ by PCR and urease test. Mucosal inflammation was evaluated on formalin-fixed tissue samples. Lesions in
the pars oesophagea were scored macroscopically. Infection was succesful in all challenged animals, with the antrum and the
fundus being predominantly positive. Infection was associated with infiltration of lymphocytes and plasma cells in the antral
mucosa, evolving to follicular gastritis. No apparent inflammation of the fundic stomach region was detected in the infected
animals. A clear link between ‘Candidatus Helicobacter suis’ and pars oesophageal lesions could not be found. 相似文献
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The proposal that saponins produced by the lily bog asphodel (Narthecium ossifragum) may be the direct cause of the hepatogenous photosensitization disease alveld seen in Norwegian lambs was investigated by comparing sapogenin levels in two control and two toxic pastures, and in faeces from lambs grazing the four pastures in the Halsa and Surnadal municipalities, Møre og Romsdal county, Norway. Generally similar levels of sapogenins, determined after hydrolysis of parent plant saponins, were found in Narthecium leaves collected in June/July 2001 from the two alveld outbreak areas and two nearby control areas. Differences in the median sapogenin levels determined for leaf samples in outbreak and control areas were not statistically significant. The total level of free and conjugated sapogenins in faeces recovered from the rectums of lambs grazing the outbreak and control pastures areas varied greatly. The results obtained do not support the hypothesis that a dose–response relationship exists between Narthecium saponin levels and the occurrence of alveld outbreaks. 相似文献
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Martin G. Maquivar Carlos S. Galina Jaime R. Galindo Sandra Estrada Rafael Molina German David Mendoza 《Tropical animal health and production》2010,42(4):555-560
In order to evaluate the effect of protein supplementation on the productive and reproductive performance of heifers, 45 Bos indicus × Bos taurus heifers, 673 ± 146 days of age and weighing about 340 kg, were divided into two groups. The control group (n = 23) continued without supplementation, but the supplemented group (SG; n = 22) received concentrate at a rate of 1% BW kg per day. Animals were adapted to the concentrate over a 15-day period and
then supplemented for 30 days, after which estrus was synchronized using a progesterone implant. Back fat thickness (BFT)
was assessed by ultrasound. The SG had better average daily weight gain than the nonsupplemented group (0.63 ± 0.16 vs. 0.51 ± 0.13 kg/day,
P < 0.05) and tended to show a better percentage ovulation (P < 0.10, 77% vs. 57%, respectively). However, this tendency was not reflected in the final pregnancy rates. No differences
were observed between groups in dry matter intake and body condition scores. SG with a high BFT (>0.70 cm) showed a better
ovulation percentage than those with low BFT (<0.69 cm; P < 0.01). Additionally, the pregnancy rate of supplemented heifers with high BFT tended to be better than heifers with low
BFT in this same group (67% vs. 30%, respectively, P < 0.10). It is concluded that protein supplementation improved reproductive performance and that ultrasonography to measure
BFT may be used to estimate reproductive performance. 相似文献
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蜂箱昨天收到了,已经组装好,非常满意,非常感谢,以后有需要再联系您!我非常喜欢阅读龚教授以及你们的文章,最近在搜集《蜜蜂杂志》上的“《中蜂饲养原理与方法》的问与答”、“《蜜蜂饲养原理与方法》的问与答”以及“《〈蜜蜂饲养原理与方法〉的问与答》继往开来”等上面的相关文章,准备形成“连续剧”,把大家问的问题理一理,好好对照读,希望在新的一年里自己的养蜂技术能得到较大的提高。 相似文献
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