Physico-chemical typology of the biogenic structures of termites and earthworms: a comparative analysis

A comparative analysis of 14 physico-chemical and organic parameters was conducted on three types of biogenic structures: earthworm casts and crop sheetings built by two fungus-growing termites (Ancistrotermes guineensis and Odontotermes nilensis) and compared to the neighbouring soil in a mango orchard in the Thiès region of Senegal. A principal component analysis shows clearly that the biogenic structures were differentiated from the neigbhouring soils in their content of organic components. The amino sugar contents of biogenic structures were 3-5 times higher than those measured in soil. In these structures, an increase in the contents of phenols and proteins was also observed. In contrast, their physical and chemical characteristics (texture, cationic exchanges capacity) were little different from those of the soil with the exception of their much lower water-retention capacity. Differences were also noted between the biogenic structures: the structures built by the two species of termite show more similarities with each other than with the casts. This result was confirmed by a similarity dendrogram which was used to graduate oppositions between samples. Casts were different from termite sheetings primarily in their C and N contents which were twice as high. Conversely, the contents of proteins in termite sheetings were higher than those measured in casts. Altogether, the results suggest that, in the biogenic structures, the organic content is stored and protected from mineralization.     

Comparative analysis of Andiodrilus pachoensis casts in forests and pastures of South-Eastern Amazon (Brazil)

Soil ecosystem engineers produce biogenic structures (casts, mounds, galleries) that strongly affect soil processes. A comparative analysis of the physical characteristics of the casts produced by the earthworm Andiodrilus pachoensis was carried out in four pastures sowed with Brachiaria brizantha and four primary forests on the deforestation front of the Amazonian forest. In both systems, we recorded surface cast density and spatial patterns as well as surface cast physical properties. Cast and vegetation distributions were mapped within plots of sizes ranging from 25 to 50 m², and cast production was monitored during 2 months. Cast spatial distribution varied greatly among plots and across spatial scales ranging from regular at small spatial scales (0–20 cm) to clumped for larger distance ranges (>80 cm). Cast density was not significantly correlated with grass tuft density and their production was independent from the presence of grass tufts in pastures or litter quantity in forests. Although bulk soil properties (pH, C content, structural stability, etc) differed between pastures and forests, cast physical properties did not differ significantly among the studied ecosystem.     

Habitat use and activity patterns of larval and adult Cantharis beetles in arable land

Generalist predators play a key role in agriculturally and environmentally sustainable systems of pest control. A detailed knowledge on their ecology, however, is needed to improve management practices to maximize their service of pest control. The present study examines the habitat use and activity patterns of larval and adult Cantharis beetles that are abundant predators in arable land. Laboratory experiments revealed that sixth instar larvae of Cantharis fusca and Cantharis livida significantly preferred high relative humidity levels of 85–90% to lower ones. This can explain their preference for meadows over fields due to the more favorable microclimatic conditions in the former habitats. Surface activity of sixth instar Cantharis larvae during autumn, winter and early spring occurred at soil temperatures above 0 °C. However, no correlation between surface activity and soil temperature, air temperature or relative humidity was found above 0 °C. Catches of sixth instar Cantharis larvae within fenced pitfall traps were higher in a meadow (Mean ± S.D.; 13.8 ± 7.63 individuals m⁻²) than in a field (4.60 ± 2.89 individuals m⁻²). Mark-recapture density estimations for sixth instar larvae indicated mean densities of 25.9 ± 5.63 (field) and 42.8 ± 16.0 individuals m⁻² (meadow). The same pattern was found for adult emergence rates in the field (0.17 ± 0.39 adults m⁻²) and meadow (1.83 ± 1.17 adults m⁻²) as well as for adult densities in the vegetation (field 4.89 ± 3.62 adults 60 m⁻²; meadow 12.5 ± 11.2 adults 60 m⁻²). It is concluded that especially in winter elements that provide plant cover should be incorporated in arable fields to enhance larval cantharid population densities and to attract them from their prime grassland habitats into arable sites.     

Influence of acetamiprid on soil enzymatic activities and respiration

The effect of a new pesticide, acetamiprid, applied at normal field concentration (0.5 mg kg⁻¹ dried soil) and at high concentration (5 and 50 mg kg⁻¹ dried soil), on soil enzyme activities and soil respiration in upland soil was studied. The results showed that acetamiprid had a strong negative influence on soil respiration and phosphatase activity, and the enzyme activities in soil treated with 5 and 50 mg kg⁻¹ dry soil were significantly (P < 0.05) lower than the CK over the course of incubation. The 7-, 14-, and 35-day EC10 for phosphatase were 11, 15, and 11 mg kg⁻¹ dry soil, respectively. The 21-day EC10 and EC50 for soil respiration was 0.005 and 83 mg kg⁻¹ dry soil. The activity of dehydrogenase was enhanced after acetamiprid application for 2 weeks and the enzyme activities in samples treated with 0.5, 5 and 50 mg kg⁻¹ dry soil was about 2.5-, 1.5- and 2-fold to that of the control on sample day 28. Variance of urease and catalase had no distinct relationship with the application concentration. The activity of proteinase was not significantly affected within the first 2 weeks but inhibited from the fourth week after acetamiprid application and was only 0.45-fold to that of the control on sample day 28. Overall, acetamiprid at normal field dose would not pose a toxicological threat to soil enzymes, but a certain potential threat to soil respiration.     

Seasonal changes in the spatial structures of N2O, CO2, and CH4 fluxes from Acacia mangium plantation soils in Indonesia

We evaluated the spatial structures of nitrous oxide (N₂O), carbon dioxide (CO₂), and methane (CH₄) fluxes in an Acacia mangium plantation stand in Sumatra, Indonesia, in drier (August) and wetter (March) seasons. A 60 × 100-m plot was established in an A. mangium plantation that included different topographical elements of the upper plateau, lower plateau, upper slope and foot slope. The plot was divided into 10 × 10-m grids and gas fluxes and soil properties were measured at 77 grid points at 10-m intervals within the plot. Spatial structures of the gas fluxes and soil properties were identified using geostatistical analyses. Averaged N₂O and CO₂ fluxes in the wetter season (1.85 mg N m⁻² d⁻¹ and 4.29 g C m⁻² d⁻¹, respectively) were significantly higher than those in the drier season (0.55 mg N m⁻² d⁻¹ and 2.73 g C m⁻² d⁻¹, respectively) and averaged CH₄ uptake rates in the drier season (−0.62 mg C m⁻² d⁻¹) were higher than those in the wetter season (−0.24 mg C m⁻² d⁻¹). These values of N₂O fluxes in A. mangium soils were higher than those reported for natural forest soils in Sumatra, while CO₂ and CH₄ fluxes were in the range of fluxes reported for natural forest soils. Seasonal differences in these gas fluxes appears to be controlled by soil water content and substrate availability due to differing precipitation and mineralization of litter between seasons. N₂O fluxes had strong spatial dependence with a range of about 18 m in both the drier and wetter seasons. Topography was associated with the N₂O fluxes in the wetter season with higher and lower fluxes on the foot slope and on the upper plateau, respectively, via controlling the anaerobic-aerobic conditions in the soils. In the drier season, however, we could not find obvious topographic influences on the spatial patterns of N₂O fluxes and they may have depended on litter amount distribution. CO₂ fluxes had no spatial dependence in both seasons, but the topographic influence was significant in the drier season with lowest fluxes on the foot slope, while there was no significant difference between topographic positions in the wetter season. The distributions of litter amount and soil organic matter were possibly associated with CO₂ fluxes through their effects on microbial activities and fine root distribution in this A. mangium plantation.     

Wood biochar increases nitrogen retention in field settings mainly through abiotic processes

Nitrogen (N) is an essential element associated with crop yield and its availability is largely controlled by microbially-mediated processes. The abundance of microbial functional genes (MFG) involved in N transformations can be influenced by agricultural practices and soil amendments. Biochar may alter microbial functional gene abundances through changing soil properties, thereby affecting N cycling and its availability to crops. The objective of this study was to assess the effects of wood biochar application on N retention and MFG under field settings. This was achieved by characterising soil labile N and their stable isotope compositions and by quantifying the gene abundance of nifH (nitrogen fixation), narG (nitrate reduction), nirS, nirK (nitrite reduction), nosZ (nitrous oxide reduction), and bacterial and archeal amoA (ammonia oxidation). A wood-based biochar was applied to a macadamia orchard soil at rates of 10 t ha⁻¹ (B10) and 30 t ha⁻¹ (B30). The soil was sampled after 6 and 12 months. The abundance of narG in both B10 and B30 was lower than that of control at both sampling months. Canonical Correspondence Analysis showed that soil variables (including dissolved organic C, NO₃⁻–N and NH₄⁺–N) and sampling time influenced MFG, but biochar did not directly impact on MFG. Twelve months after biochar application, NH₄⁺–N concentrations had significantly decreased in both B10 (4.74 μg g⁻¹) and B30 (5.49 μg g⁻¹) compared to C10 (13.9 μg g⁻¹) and C30 (17.9 μg g⁻¹), whereas NO₃⁻–N concentrations increased significantly in B30 (24.7 μg g⁻¹) compared to B10 (12.7 μg g⁻¹) and control plots (6.18 μg g⁻¹ and 7.97 μg g⁻¹ in C10 and C30 respectively). At month 12, significant δ¹⁵N of NO₃⁻–N depletion observed in B30 may have been caused by a marked increase in NO₃⁻–N availability and retention in those plots. Hence, it is probable that the N retention in high rate biochar plots was mediated primarily by abiotic factors.     

Restoration of shrub communities elevates organic carbon in arid soils of northwestern China

Artificial restoration by shrub plantation in semi-arid sandy land can increase carbon sequestration. However, little information is available on the carbon flux input to soil resulted from fine roots turnover and leaf fallen during restoration. The present study relying on the ingrowth core and sequential core methods investigated the fine-root dynamics and fine-root production of three shrub stands (dominated by Artemisia halodendron, Caragana microphylla and Salix gordejevii respectively) which have different life-forms and root architectures. The soil carbon and nitrogen stock was also estimated in the restoration, and the relative contribution of carbon input related to fine root mortality and leaf fallen was assessed. The mean standing live and dead fine-root biomass in A. halodendron stand at the primary restoration were significantly less than in C. microphylla stand at moderate restoration and S. gordejevii stand in lowland. Consistent with leaf production, fine root production showed a positive correlation with soil water content and followed the order of A. halodendron < C. microphylla < S. gordejevii. In contrast, the fine-root turnover rate was quicker in primary restoration phase (2.12 year⁻¹) than in moderate restoration phase (1.55 year⁻¹) and lowland (1.28 year⁻¹). The annual carbon and nitrogen inputs via fine root mortality and leaf fallen increased from 74.78 g C m⁻² year⁻¹ and 1.25 g N m⁻² year⁻¹ in A. halodendron stand to 189.66 g C m⁻² year⁻¹ and 1.67 g N m⁻² year⁻¹ in S. gordejevii stand. Although the share of the fine roots of A. halodendron seized a relatively smaller proportion in the net primary production compared with those in C. microphylla and S. gordejevii, the relative contribution of carbon input related to fine roots mortality in primary restoration phase was higher than in the other two shrub stands. The present study proved that the carbon input to soil by fine-root mortality considerably contributed to the restoration of soil carbon and nitrogen stock in semi-arid degraded lands.     

The influence of different vegetation patches on the spatial distribution of nests and the epigeic activity of ants (Lasius niger) on a spoil dump after brown coal mining (Czech Republic)

A study was carried out during 2001 on mine tailings in NW Bohemia aimed at describing the spatial patterns of nests distribution and epigeic activity of ants in relation to the vegetation mosaic. Lasius niger was the most abundant species of ant and its nest mounds were significantly more numerous in patches with sparse vegetation than inside dense Calamagrostis epigejos vegetation; this was particularly true for small and medium-sized nests. Small and medium nests also occurred more frequently near the edges of a given patch than in the center. Large and medium nests were randomly distributed in the area, whereas small nests had an aggregated distribution. Pitfall trapping reveal significantly higher activity of L. niger workers in tall and dense vegetation stands in comparison with low and sparse vegetation. This pattern was particularly pronounced during the peak of foraging activity in summer and was not so significant in spring or autumn. We expect that ant preferentially forage in shaded habitats during the summer months when bare soil may be too hot. The results indicated that nesting and foraging may differ in their microclimatic requirements and the formation of vegetation mosaics may be important to changes in the ant population during succession.     

Fire interacts with season to influence soil respiration in tropical savannas

Soil respiration (R_s) is the second-largest source of CO₂ to the atmosphere in terrestrial systems. In tropical savannas seasonal moisture availability and frequent fires drive ecosystem dynamics and may have a considerable impact on soil carbon (C) cycling, including R_s. In order to test the effect of fire on soil C cycling we measured R_s in annually burnt and unburnt plots in wet and dry seasons at a long-term fire experiment established in savanna woodlands of northern Australia. There was a significant interaction between season and fire, with highest rates of daily R_s (722 mmol CO₂ m⁻² d⁻¹) observed in the wet season on unburnt, leaf litter patches. The three fold higher R_s rate on unburnt plots in the wet season was due to greater root-derived respiration (R_root: 356 mmol CO₂ m⁻² d⁻¹), while smaller changes to soil-derived respiration (R_soil: 51 mmol CO₂ m⁻² d⁻¹) were simply the result of C moving through decomposition rather than combustion pathways. Relationships between instantaneous R_s and soil temperature showed hysteresis with variable direction, suggesting that season and fire treatment also influence the soil depth at which CO₂ is produced. We suggest that (1) changes to fire regimes, through active management or climate change, in tropical savannas could have an impact on R_s, and (2) the direct effect of fire on soil C cycling is limited to the removal of aboveground litter inputs.     

The phosphorus source determines the arbuscular mycorrhizal potential and the native mycorrhizal colonization of tall fescue and wheatgrass

The study assesses the effect of two phosphate (P) sources (soluble superphosphate (SP) and rock phosphate (RP)) on the arbuscular mycorrhizal potential (AMP), the root arbuscular mycorrhizal colonization (AMC) and the growth of tall fescue and wheatgrass of a grassland soil from Argentina. Mycorrhizal potential was assessed with soil samples collected from 2 years for tall fescue and wheatgrass swards before and after field plots were fertilized with 0 and 60 kg P ha⁻¹ as SP or RP. Mycorrhizal potential both at unfertilized and at RP fertilized plots was high (12–14 AM propagules g⁻¹), however fertilization with SP caused a decrease in AMP (0.70–0.95 AM propagules g⁻¹). A range of soil P between 4 and 46 mg P kg⁻¹ and a range of root AMC between 6% and 50% were obtained after fertilization with four rates of SP and RP (0, 15, 30, and 60 kg P ha⁻¹) in plots where tall fescue and wheatgrass were grown during 2 years. Soil P and root mass were higher in the top 10-cm depth than in the 20-cm of the soil profile, but AMC did not change with depth. Shoot dry matter (SDM) production of both grasses did not differ after fertilization with SP or RP, particularly at second year. The AMP positively correlated with the indigenous AMC, and they were not different between tall fescue or wheatgrass. Lineal-plateau relationships between soil P, relative SDM and AMC were established. Highest relative SDM was attained at 6.5 mg P kg⁻¹ in plots fertilized with RP, and at 15.2 mg P kg⁻¹ with SP. Variability in colonization was well accounted by the soil P (at 0–10 cm depth) fertilized with SP (r² = 0.48, P 0.01), but any relationship was found with RP. The AMC decreased with increasing available soil P from plots with SP until 18.3 mg kg⁻¹ (a decrease of 2.2% per mg P kg), after that AMC was stabilized at about 6.9%. Our study clearly showed that fertilization with SP or RP produced similar available soil P content and grasses SDM production. Mycorrhiza root colonization and propagules decreased after fertilization with SP, but fertilization with RP did not decrease mycorrhizal propagules nor colonization. It can be concluded that RP fertilization instead SP could allow obtaining acceptable tall fescue and wheatgrass yield enhancing mycorrhizal potential of soils and indigenous colonization of plants and thus maximizing the use of fertilizer.     

Effects of Cd and Pb on the survival and juvenile production of Sinella coeca and Folsomia candida

Degradation factors can cause both quantitative and qualitative changes in fauna and thus affect soil functioning. The use of bio-indicators may help to detect environmental changes. The aim of this study was to evaluate the effects of lead and cadmium on the biology (mortality, reproduction, egg hatching) of two euedaphic collembolans, Sinella coeca and Folsomia candida and to evaluate sensitivity differences between the two species. The results showed that low concentrations of lead did not cause dramatic effects on the survival and reproduction of both species. Reproduction of both species was reduced when the soil contained a nominal concentration of Pb at 1000 μg g^–1. S. coeca and F. candida showed a high sensitivity to cadmium and both their reproduction and survival were significantly reduced on exposure to 50 μg g^–1. Moreover, egg hatching of F. candida was affected by the cadmium concentrations in the soil, and by the amount of time that the eggs stayed in contact with cadmium. This study showed that the inhibition determined by Cd on F. candida juvenile production was caused in part by inhibition of deposition and, in part, by reduction of egg viability.     

Spatial heterogeneity provides organic matter refuges for soil microbial activity in the Patagonian steppe, Argentina

In arid and semi-arid ecosystems that are frequently classified as water limited, it is unclear how spatial and temporal variability of vegetation and climate could affect microbially-mediated soil processes. Our objective was to determine how aboveground spatial heterogeneity creates characteristic soil conditions that modulate microbial growth and activity in a semi-arid Patagonian steppe. In particular, we explored how micro-environmental and biogeochemical soil characteristics generated by the native vegetation could control soil β-glucosidase activity. Both life-form (shrubs, grasses, mosses and bare soil) and season exerted strong controls on all measured abiotic (soil temperature and gravimetric soil water content, inorganic nitrogen, pH and total C and N) and biotic (microbial biomass C and β-glucosidase activity) soil characteristics. Partial correlation between β-glucosidase activity and extracellular organic C (EOC) was high across seasons (r = 0.5; P < 0.001) while soil water content did not correlate with soil enzymatic activity (r = 0.09; P > 0.05). We postulate that labile soil carbon rather than water availability functions as a principal limitation of microbial activity in this semi-arid ecosystem, and the distribution of this carbon is, in large part, determined by the patchy distribution of vegetation.     

Is Hormogaster elisae (Oligochaeta,Hormogastridae) a predator of mites and springtails?

The aim of this work was to determine whether the endogeic earthworm Hormogaster elisae [1] is involved in the active or passive predation of microarthropods at El Molar (Madrid, Spain). Different techniques were employed to study the gut content, and the casts of H. elisae earthworms cultivated in the laboratory. The casts consisted mainly of mineral particles and plant remains as well as a few microarthropods, nematodes and their remains. The gut contents were similar in composition, although no microarthropod remains were found, except for a single springtail (order Poduromorpha) in one earthworm's gizzard. The results suggest that H. elisae may accidentally ingest microarthropods along with soil. The microarthropods found in the casts may have colonized them after their deposition since none were found in isolated casts.     

Winter soil CO2 efflux and its contribution to annual soil respiration in different ecosystems of a forest-steppe ecotone, north China

Most soil respiration measurements are conducted during the growing season. In tundra and boreal forest ecosystems, cumulative winter soil CO₂ fluxes are reported to be a significant component of their annual carbon budgets. However, little information on winter soil CO₂ efflux is known from mid-latitude ecosystems. Therefore, comparing measurements of soil respiration taken annually versus during the growing season will improve the accuracy of ecosystem carbon budgets and the response of soil CO₂ efflux to climate changes. In this study we measured winter soil CO₂ efflux and its contribution to annual soil respiration for seven ecosystems (three forests: Pinus sylvestris var. mongolica plantation, Larix principis-rupprechtii plantation and Betula platyphylla forest; two shrubs: Rosa bella and Malus baccata; and two meadow grasslands) in a forest-steppe ecotone, north China. Overall mean winter and growing season soil CO₂ effluxes were 0.15-0.26 μmol m⁻² s⁻¹ and 2.65-4.61 μmol m⁻² s⁻¹, respectively, with significant differences in the growing season among the different ecosystems. Annual Q₁₀ (increased soil respiration rate per 10 °C increase in temperature) was generally higher than the growing season Q₁₀. Soil water content accounted for 84% of the variations in growing season Q₁₀ and soil temperature range explained 88% of the variation in annual Q₁₀. Soil organic carbon density to 30 cm depth was a good surrogate for SR₁₀ (basal soil respiration at a reference temperature of 10 °C). Annual soil CO₂ efflux ranged from 394.76 g C m⁻² to 973.18 g C m⁻² using observed ecosystem-specific response equations between soil respiration and soil temperature. Estimates ranged from 424.90 g C m⁻² to 784.73 g C m⁻² by interpolating measured soil respiration between sampling dates for every day of the year and then computing the sum to obtain the annual value. The contributions of winter soil CO₂ efflux to annual soil respiration were 3.48-7.30% and 4.92-7.83% using interpolated and modeled methods, respectively. Our results indicate that in mid-latitude ecosystems, soil CO₂ efflux continues throughout the winter and winter soil respiration is an important component of annual CO₂ efflux.     

Effects of simulated nitrogen deposition on soil respiration components and their temperature sensitivities in a semiarid grassland

Nitrogen (N) deposition to semiarid ecosystems is increasing globally, yet few studies have investigated the ecological consequences of N enrichment in these ecosystems. Furthermore, soil CO₂ flux – including plant root and microbial respiration – is a key feedback to ecosystem carbon (C) cycling that links ecosystem processes to climate, yet few studies have investigated the effects of N enrichment on belowground processes in water-limited ecosystems. In this study, we conducted two-level N addition experiments to investigate the effects of N enrichment on microbial and root respiration in a grassland ecosystem on the Loess Plateau in northwestern China. Two years of high N additions (9.2 g N m⁻² y⁻¹) significantly increased soil CO₂ flux, including both microbial and root respiration, particularly during the warm growing season. Low N additions (2.3 g N m⁻² y⁻¹) increased microbial respiration during the growing season only, but had no significant effects on root respiration. The annual temperature coefficients (Q₁₀) of soil respiration and microbial respiration ranged from 1.86 to 3.00 and 1.86 to 2.72 respectively, and there was a significant decrease in Q₁₀ between the control and the N treatments during the non-growing season but no difference was found during the growing season. Following nitrogen additions, elevated rates of root respiration were significantly and positively related to root N concentrations and biomass, while elevated rates of microbial respiration were related to soil microbial biomass C (SMBC). The microbial respiration tended to respond more sensitively to N addition, while the root respiration did not have similar response. The different mechanisms of N addition impacts on soil respiration and its components and their sensitivity to temperature identified in this study may facilitate the simulation and prediction of C cycling and storage in semiarid grasslands under future scenarios of global change.     

Biological activation of hydrous ferric oxide for reduction of hexavalent chromium in the presence of different anions

Shewanella alga BrY, a dissimilatory iron reducing bacterium (DIRB), transformed inert ferric oxides that are common in sediments, aquifer material and passivated permeable reactive iron barriers (PRBs), producing dissolved and sorbed Fe(II) capable of rapidly reducing and immobilizing Cr(VI). The effect of groundwater chemistry on the formation and reactivity of such microbial-produced, abiotic reductants was investigated. Batch reactors with high carbonate concentration (10 mM) were the most reactive, removing 66.0% ± 2.8 of Cr (VI) (76 mg/l) from liquid phase within 5 min. Treatments with high concentrations of sulfate (5.2 mM), chloride (10 mM), phosphate (1 mM) or silica (0.75 mM) were less reactive (about 40% removal). Loss of reactivity was observed possibly due to oxidation of Fe(II) (sorbed and dissolved) by Cr(VI). Normalization of Cr(VI) removal to the mass of biogenic solid present showed the following molar Cr/Fe ratios in solid phase: 0.185 ± 0.041 (carbonate), 0.146 ± 0.013 (sulfate), 0.092 ± 0.010 (silica), 0.075 ± 0.012 (phosphate) and 0.062 ± 0.012 (chloride). Overall, these results show that bacterial transformation of inert ferric oxides can contribute to the (abiotic) natural attenuation of Cr(VI) in and around PRBs, and that groundwater chemistry is an important determinant of biogenic solids reactivity.     

Spatial patterns of soil biological and physical properties in a ridge tilled and a ploughed Luvisol

The present study was conducted to determine the spatial heterogeneity of bulk density, soil moisture, inorganic N, microbial biomass C, and microbial biomass N in the ridge tillage system of Turiel compared to conventional mouldboard ploughing on three sampling dates in May, July, and August. The soil sampling was carried out under vegetation representing the ridge in a high spatial resolution down the soil profile. Bulk density increased with depth and ranged from 1.3 g cm⁻³ at 10 cm depth to 1.6 g cm⁻³ at 35 cm in ploughed plots and from 1.0 g m⁻³ at 5 cm to 1.4 g m⁻³ at 35 cm in the ridges. In the ploughed plots, the contents of microbial biomass C and microbial biomass N remained roughly constant at 215 and 33 μg g⁻¹ soil, respectively, throughout the experimental period. The microbial biomass C/N ratio varied in a small range around 6.4. In the ridged plots, the contents of microbial biomass C and microbial biomass N were 5% and 6% higher compared to the ploughed plots. Highest microbial biomass C contents of roughly 300 μg g⁻¹ soil were always measured in the crowns in July. The lowest contents of microbial biomass C of 85–137 μg g⁻¹ soil were measured in the furrows. The ridges showed strong spatial heterogeneity in bulk density, soil water content, inorganic nitrogen and microbial biomass.     

Methyl-mercury affects microbial activity and biomass,bacterial community structure but rarely the fungal community structure

Monomethyl-mercury is one of the most toxic compounds. Methylation of Hg usually appears under anoxic conditions. In Swiss forest soils, methyl-Hg concentrations of up to 3 μg kg⁻¹ soil dw have been observed, but the impact of methyl-Hg on soil microorganisms have rarely been examined so far. In this study, we investigated the effect of increasing concentrations of methyl-Hg (0, 5, 20, 90 μg kg⁻¹ soil dw) on the microbial communities in various forest soils differing in their physico-chemical properties. Experiments were conducted in microcosms under controlled conditions and the basal respiration (BR), the microbial biomass carbon (MBC) and the bacterial and fungal community structures using T-RFLP-profiling were investigated. BR was significantly affected by methyl-Hg. In general, the BR increased with increasing methyl-Hg concentrations, whereas the MBC was significantly reduced. Bacterial communities were more sensitive to methyl-Hg than fungal communities. In five out of seven soils, the bacterial community structures differed significantly between the treatments whereas the fungal communities did not. The impact of methyl-Hg on the soil bacterial communities was site specific. In one soil, a methyl-Hg concentration of already 5 μg kg⁻¹ soil dw significantly affected the relative abundance of 13% bacterial operational taxonomic units (OTU), whereas in other soils concentrations of even 90 μg kg⁻¹ soil dw rarely affected the abundance of OTUs. In this study, for the first time, the impact of methyl-Hg on soil bacterial and fungal communities in forest soils was assessed. We showed that its impact strongly depends on the physico-chemical conditions of the soil and that bacterial communities were more sensitive to methyl-Hg than fungi.     

Effects of humic acids from vermicomposts on plant growth

The interactions between earthworms and microorganisms can produce significant quantities of plant growth hormones and humic acids which act as plant regulators. Experiments were designed to evaluate the effects of humic acids extracted from vermicompost and compare them with the action of commercial humic acid in combination with a commercial plant growth hormone, indole acetic acid (IAA) which is a commonly found in vermicomposts. In the first experiments, humic acids were extracted from cattle, food and paper waste vermicomposts. They were applied to a plant growth medium, Metro-Mix360 (MM360), at rates of 0, 250 or 500 mg humates kg⁻¹ dry wt. of MM360, to marigold, pepper, and strawberry plants in the greenhouse. Substitution of humates ranging from 250 to 1000 mg kg⁻¹ MM360 increased the growth of marigold and pepper roots, and increased the growth of roots and numbers of fruits of strawberries significantly. In other experiments, humic acids extracted from food waste vermicomposts were applied at a rate of 500 mg kg⁻¹ dry wt. of MM360, singly or in combination with IAA at a rate of 10⁻⁵ μM, to pepper seedlings. This experiment was designed to compare the differences in effects between the most effective dosage rate of humic acid from food waste, a phytohormone (IAA), and a commercial source of humic acid. The numbers of pepper flowers and fruits increased significantly in response to treatment with humic acid, IAA and a combination of humic acid and IAA. Peppers treated with humic acids extracted from food waste vermicomposts produced significantly more fruits and flowers than those treated with commercially-produced humic acids.     

Determining nitrogen fate by hydrological pathways and impact on carbonate weathering in an agricultural karst watershed

Identifying the nitrogen (N) fate is complicated and a great challenge in karst watersheds because of the co-existence of natural pools and anthropogenic sources. The objective of the study was to use stable isotopic composition of dual-isotope (δ¹⁵N_Nitrate and δ¹⁸O_Nitrate) and LOADEST model approaches to trace N sources, pathways in karst watershed. The study was conducted in the Houzhai watershed, which is a typical agricultural karst watershed from July 2016 to August 2018, to reveal the N fate and the coupled carbon(C)–N processes occurring in the riverine-watershed with agricultural activities. We found that the wet deposition of total nitrogen (TN) flux was 33.50 kg hm⁻²·a⁻¹ and dissolved nitrogen (DN) flux was 21.66 kg hm⁻²·a⁻¹. The DN runoff loss was 2.10 × 10⁵ kg·a⁻¹ and the loss of DN during the wet season accounted for 95.4% over a year. In the wet season, NO₃⁻-N daily efflux was 977.62 ± 516.66 kg ha⁻¹·day⁻¹and 248.77 ± 57.83 kg ha⁻¹·day⁻¹ in the dry season. The NH₄⁺-N efflux was 29.17 ± 10.50 kg ha⁻¹·day⁻¹ and 4.42 ± 3.07 kg ha⁻¹·day⁻¹ in the wet and dry seasons, respectively. The main form output load of N was NO₃⁻-N which was more than 30 times as much as NH₄⁺-N output loss. The NO₃⁻-N caused by rainfall contributed 11.82%–53.61% to the export load. Nitrate from soil contributed over 94% of the N to Houzhai river caused by N leaching. In addition, manure and farmland soil were the main sources of groundwater in the Houzhai watersheds, the contribution rates were 25.9% and 22.5%. The chemical N fertilizers affected carbonate weathering strongly, and the HCO₃⁻ flux caused by nitrification due to N fertilizers application in soil accounted for 23.5% of the entire watershed. This study suggested that carbonate weathering may be influenced by nitrogen nitrification in the karst watershed.