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刚察地区牛传染性鼻气管炎的血清学调查 总被引:9,自引:0,他引:9
杨春明 《青海畜牧兽医杂志》2003,33(2):39-39
牛传染性鼻气管炎是由病毒引起的一种急性呼吸道传染病 ,其特征为生殖道感染、结膜炎、脑膜炎和流产等多种病状 ,仅感染牛。为了了解牛传染性鼻气管炎在刚察县的流行情况 ,1998年我们在进行牛羊布氏杆菌病血检的同时 ,应用间接血凝试验 ,对刚察地区的牛进行了血清学调查。1 材料和方法1 1 材料 :抗原、阳性血清均系中国农业科学院兰州兽医研究所提供 ,被检血清采自全县 5乡 1场以及青海省三角城种羊场、青海湖农场的牦牛、黄牛和犏牛。1 2 方法 :间接血凝试验 ,按中国农业科学院兰州兽医研究所制定的方法进行操作与判定。2 检验结果共检… 相似文献
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从青海省互助、玛多、海晏、玉树、贵德、德令哈、共和等14个地区采集牛血清样品420份,应用酶联免疫吸附试验(ELISA)调查了青海省牛传染性鼻气管炎和病毒性腹泻黏膜病的感染情况。结果在被检牛血清420份样品中,共检出阳性血清样品228份,平均阳性率为50.67%(228/420);在被检牦牛血清180份样品中,共检出阳性血清样品1份,平均阳性率0.56%(1/180)。 相似文献
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为掌握青海省大通种牛场和海晏县牦牛病毒性腹泻/黏膜病、传染性鼻气管炎的感染和流行情况,2010年3月至5月在大通种牛场和海晏地区采集252份牦牛血清样品,应用定量酶联免疫吸附试验(ELISA)对牦牛病毒性腹泻/黏膜病和传染性鼻气管炎的进行了血清抗体检测。结果显示,大通种牛场牦牛群中牛病毒性腹泻/黏膜病阳性率为23.42%,传染性鼻气管炎阳性率为65.45%;海晏县牦牛群中牛病毒性腹泻/黏膜病阳性率为19.86%,传染性鼻气管炎阳性率为4.96%。 相似文献
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R F Kahrs 《Journal of the American Veterinary Medical Association》1968,153(12):1652-1655
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Trials were conducted on rabbits and cattle to compare the immunizing effectiveness of the subunit vaccine against infectious bovine rhinotracheitis (IBR), representing antigens separated by the solubilization of the IBR virus-infected cells by means of Triton X-100 with oil adjuvant, with the inactivated oil IBR vaccine. The rabbits inoculated and re-vaccinated with both vaccines in an interval of three weeks produced neutralizing antibodies in medium titres, the values of these antibodies were balanced in both groups. Cattle immunized with the subunit vaccine reacted to the inoculation and re-vaccination by producing serum antibodies of higher titres, as compared with the cattle inoculated with the virus vaccine; secretory antibodies were detected only after re-vaccination and had balanced values in both test groups. After intranasal infection with the virulent virus performed after 14 days from re-vaccination, the calves inoculated with the subunit and virus vaccines were protected against clinical disease whereas the non-inoculated control calves fell ill with symptoms characteristic of IBR. The immunized animals of both experimental groups had a smaller amount of virus p.i. in nasal secretions and for a shorter time than the control non-inoculated calves. The intensity of multiplication and persistence of infectious virus excretion were the same in both experimental groups. 相似文献