基于复合指纹法的九乡河小流域泥沙来源解析

在一个农业和采矿活动较多的小流域,分析了不同用地类型,包括农田、林地、采矿用地等潜在泥沙来源的土壤及泥沙的地球化学性质特征,利用复合指纹和多元混合模型研究了流域输出泥沙的主要来源及相对贡献。研究表明:该方法对不同泥沙来源的正确判别率最高达到87.5%,模型拟合优度达0.93。各泥沙样品中来源于农田的相对贡献为25.3%~65.2%,来源于矿山用地的相对贡献为34.8%~74.7%,来源于林地的泥沙总体上不到0.1%。指纹识别技术在地表物质均一的小流域也能有效识别泥沙的来源。     

复合指纹识别技术定量示踪流域泥沙来源

土壤侵蚀导致水土资源及土地生产力的破坏和损失,泥沙淤积危害及其引发的一系列水环境效应已成为当前及以后一段时期内研究的热点和重点。开展流域(河流)泥沙来源研究,查明入塘、河、库泥沙通量,定量识别泥沙来源具有重要现实指导意义。选取了一个由山坪塘控制出口的封闭式农业单元小流域(10.7 hm2),开展复合指纹识别技术定量辨析塘库沉积泥沙来源新尝试。据流域现状,定性划分了3种泥沙来源,即旱地、水田、林草地,并分别于塘库中部采取A、B、C三柱表层沉积泥沙;结合复合指纹识别技术定量解析了塘库表层沉积泥沙来源。研究表明,塘库沉积泥沙各来源相对输沙贡献分别为旱地84%、水田14%、林草地2%,复合指纹识别技术能较好地辨析小流域泥沙来源。     

基于指纹识别技术的小流域泥沙来源研究进展

泥沙来源判别是小流域土壤侵蚀研究的重点,也是难点。复合指纹识别技术因其结果直接、准确度高、作业快等特点,在国内外泥沙来源研究中得到大量应用,并迅速发展。复合指纹识别技术是在划分潜在侵蚀区的基础上,采集侵蚀区土壤样和流域出口泥沙样,室内分析土壤样和泥沙样指纹因子,利用相关统计检验方法筛选最佳指纹因子组合,利用数值转换模型将最佳指纹因子浓度转换为各侵蚀区产沙比例。主要通过阐述指纹识别技术泥沙来源研究步骤,总结现有可靠性检验方法,归纳研究对象等方面,综述基于复合指纹识别技术的泥沙来源研究进展。重点介绍了复合指纹识别技术最佳指纹因子组合筛选和数值转换模型,并指出复合指纹识别技术存在的问题。     

基于不同模型不同指纹因子的东北黑土区小流域泥沙来源分析

基于指纹识别技术计算了东北黑土区典型小流域不同侵蚀产沙源地的泥沙贡献比。通过分析农地、林地、草地表层土以及侵蚀沟样品中的33种物质,使用非参数检验和多元判别分析筛选出包括P、Ce、Ga、Rb和137Cs组成的最优复合指纹因子,并将放射性核素137Cs和210Pbex作为第2组指纹因子,将最优复合指纹因子中的单个因子分别作为单因子,作为第3组指纹因子,分别利用多元混合线性模型、Bayesian模型和单因子解析解等泥沙来源指纹分析方法计算了表层土和侵蚀沟的相对泥沙贡献比。结果表明:基于不同模型不同指纹因子的泥沙来源贡献比结果虽不尽相同,但无重大差别。利用多元混合线性模型计算时,由放射性元素137Cs和210Pbex作为指纹因子计算的泥沙来源(表层土47.5%,侵蚀沟52.5%),与最优复合指纹因子计算的泥沙来源(表层土44.6%,侵蚀沟55.4%)基本一致;利用Bayesian模型计算时,由放射性元素137Cs和210Pbex作为指纹因子计算的表层土和侵蚀沟的泥沙贡献比约各占1/2,而利用最优复合指纹因子计算得到的泥沙贡献比中,表层土(58.8%)多于侵蚀沟(41.2%);以复合指纹因子中单个因子为指纹因子计算解析解,P、Ga、Ce、137Cs 4个因子的判别能力较强,能有效判别泥沙物源区;为保证泥沙贡献比计算结果的精确性,有必要确定各模型的计算精度,并挖掘具体的影响因素,调整参数或算法,为模型改进提供依据。研究发现,面积占比不足1%的侵蚀沟贡献了流域近1/2的泥沙,表明侵蚀沟发育引起的土壤流失不容小觑,应加强对该区侵蚀沟道的治理。     

泥沙来源“指纹”示踪技术研究综述

泥沙来源"指纹"示踪技术是综合研究流域土壤侵蚀和泥沙输移的新方法。泥沙来源"指纹"示踪技术基于流域侵蚀产沙过程划分潜在物源类型,根据物源特性筛选具有诊断能力的"指纹"性质,通过定量转换模型建立流域出口泥沙与内部潜在物源间的"指纹"联系,定量描述各潜在物源对流域出口产沙的相对贡献;结合悬移质或沉积泥沙通量监测,定量分析各潜在物源对流域产沙的绝对贡献量及流域侵蚀产沙时、空变化特征。通过综述泥沙来源"指纹"示踪技术的理论基础及实施框架,流域尺度潜在物源类型,泥沙"指纹"因子类别、分布特点及诊断能力,泥沙来源复合"指纹"示踪技术的研究进展,指出泥沙来源"指纹"示踪技术的局限性,并对泥沙来源"指纹"示踪技术进行展望。     

西南喀斯特小流域地表、地下河流细粒泥沙来源的137Cs和磁化率双指纹示踪研究

在黔中喀斯特高原农林复合生态系统峰丛谷地小流域,利用¹³⁷Cs和磁化率双指纹因子对碳酸盐岩表层土壤(坡地和坝地)、深层土壤(沟道和洞穴/裂隙)以及碎屑岩夹层岩屑3类来源开展指纹特征分析,通过多元混合模型对流域暴雨过程侵蚀悬移质泥沙进行指纹复合示踪,并计算小流域地表及地下出口输出泥沙的主要来源及相对贡献率。结果表明:¹³⁷Cs和磁化率在碳酸盐岩表层土壤、深层土壤以及碎屑岩夹层岩屑3类来源存在显著差异,流域内表层土壤、深层土壤和碎屑岩夹层岩屑的¹³⁷Cs和磁化率平均含量分别为3.39 Bq/kg和310.07×10^-8 m^3/kg、0.50 Bq/kg和180.69×10^-8 m^3/kg、0 Bq/kg和7.02×10^-8 m^3/kg。表层土壤、深层土壤和碎屑岩夹层岩屑对流域地表出口泥沙的相对贡献分别为16.2%,4.3%和79.5%,拟合优度为99.97%;表层土壤、深层土壤和碎屑岩夹层岩屑对流域地下出口泥沙的相对贡献分别为<0.1%,37.9%和62.1%,拟合优度为83.80%。喀斯特流域泥沙来源区别于其他碎屑岩流域具有的特点为:(1)碳酸盐岩风化表层土壤来源少,对河流泥沙贡献小,主要贡献于地表河流。(2)无论流域地表出口还是地下出口,河流泥沙来源主要为流域中碳酸盐岩所夹的少量(地面物质组成<10%)的碎屑岩夹层岩屑。(3)深层土壤略有贡献,地下河流贡献比例高于地表河流。另外,¹³⁷Cs和磁化率可作为双指纹示踪物较好地示踪喀斯特小流域地表、地下河流泥沙来源和确定相对贡献率。     

水平沟整地措施对黄土丘陵区草原土壤水分动态平衡的影响

三峡水库消落带是库区水域与周边陆地环境的关键过渡地带,周期性反季节干湿交替使其具有强烈的物质交换特征。辨析消落带泥沙及其吸附的颗粒态磷的来源对消落带土壤污染防治和环境效应评估以及三峡水环境保护具有重要意义。以三峡库区汝溪河支流不同高程（145～155、>155～165、>165～175 m）消落带为研究对象,运用复合指纹技术查明,消落带泥沙中颗粒态磷的主要来源是长江干流悬移质和汝溪河上游悬移质。淹水期间长江干流江水顶托引起的泥沙沉积是颗粒态磷的主要来源,在>165～175 m高程带对颗粒态磷的贡献达到最大（54.5%）。雨季初期支流上游悬移质对145～155 m高程消落带的颗粒态磷贡献最大（51.6%）,而随高程的增加贡献率减少。消落带上方的土壤侵蚀产沙主要堆积在>155～165和>165～175 m高程范围内,导致消落带上方土壤对泥沙和颗粒态磷的贡献率都随高程的增加而增加。     

三峡水库典型支流消落带泥沙颗粒态磷复合指纹示踪研究

三峡水库消落带是库区水域与周边陆地环境的关键过渡地带,周期性反季节干湿交替使其具有强烈的物质交换特征。辨析消落带泥沙及其吸附的颗粒态磷的来源对消落带土壤污染防治和环境效应评估以及三峡水环境保护具有重要意义。以三峡库区汝溪河支流不同高程（145～155、>155～165、>165～175 m）消落带为研究对象,运用复合指纹技术查明,消落带泥沙中颗粒态磷的主要来源是长江干流悬移质和汝溪河上游悬移质。淹水期间长江干流江水顶托引起的泥沙沉积是颗粒态磷的主要来源,在>165～175 m高程带对颗粒态磷的贡献达到最大（54.5%）。雨季初期支流上游悬移质对145～155 m高程消落带的颗粒态磷贡献最大（51.6%）,而随高程的增加贡献率减少。消落带上方的土壤侵蚀产沙主要堆积在>155～165和>165～175 m高程范围内,导致消落带上方土壤对泥沙和颗粒态磷的贡献率都随高程的增加而增加。     

森林火灾流域侵蚀泥沙来源指纹技术研究进展

指纹识别技术是量化流域侵蚀泥沙来源的有效手段,如何应用于火烧流域,仍有一些问题值得探讨。通过回顾相关文献,总结森林火灾通过植被、土壤和灰烬加剧土壤侵蚀的机制,介绍应用指纹技术研究火烧流域泥沙来源的案例,重点分析放射性核素、矿物磁性、物理性质、地球化学元素和有机组分等指纹因子在火烧迹地的含量和性质变化,论述各类指纹因子在火烧流域泥沙来源研究中的适用性,并提出未来应重点关注指纹技术的物理基础、火灾后土壤性质的时空变化规律、指纹因子稳定性验证、燃烧灰烬的影响以及大粒径泥沙的识别等问题。为促进火烧迹地指纹识别技术的研究、理解森林火灾对于流域产沙格局的影响、提升火灾流域水土保持和生态修复的有效性提供理论依据。     

复合指纹法定量示踪西南岩溶洼地小流域泥沙来源

岩溶洼地小流域具有岩溶地区典型流域特征,作为一个独立的侵蚀产沙单元,开展岩溶洼地小流域泥沙来源研究,对于查明洼地泥沙的来源,研究土壤侵蚀过程,发挥着重要的作用.选择重庆青木关岩溶槽谷区的典型洼地,采用复合指纹示踪技术等方法,确定岩溶洼地泥沙来源的地球化学元素指纹特征,得出了重庆岩典型岩溶洼地的沉积泥沙来源和贡献比例.结...     

低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响

【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。     

Pasture Vegetation Elemental Analysis by Laser-Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Macroinvertebrates in North American tallgrass prairie soils: effects of fire, mowing, and fertilization on density and biomass

The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.     

Variation in quality parameters between and within 14 Nordic tree fruit and berry species

Abstract

A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species.     

Potassium Fixation and Release Characteristics in Rhizosphere and Nonrhizosphere Soils for a Rapeseed–Rice Cropping Sequence

Potassium (K) fixation and release in soil are important factors in the long-term sustainability of a cropping system. Changes in K concentration and characteristics of K fixation and release in rhizosphere and nonrhizosphere soils in the rapeseed (Brassica napus L.)–rice (Oryza sativa L.) rotation were investigated using a rhizobox system. The concentrations of different forms of K in both rhizosphere and nonrhizosphere soils decreased with plants compared to without plants, regardless of K fertilizer application. Potassium uptake by crops mainly came from the rhizosphere soil. In the treatment without K fertilizer (–K), the main form of K supplied by the soil to the crops was 1.0 mol L^?1 nitric acid (HNO₃) nonextractable K, followed by nonexchangeable K, and then exchangeable K. In the treatment with K fertilizer (+K), the main K forms supplied by the soil to the crops were exchangeable K and nonexchangeable K. The amount and rate of K fixation after one cycle of the rapeseed–rice rotation was greater in rhizosphere soil than in nonrhizosphere soil. The amount and rate of K fixation of soil in the +K treatment were significantly less than in the –K treatment. The cumulative amounts of K released with 1.0 mol L^?1 ammonium acetate (NH₄OAc) and 1.0 mol L^?1 HNO₃ extraction increased with the increasing numbers of extractions, but the K-releasing power of soil by successive extraction decreased gradually and finally became almost constant. The release of K was less in rhizosphere soil than in nonrhizosphere soil. The release of K in the +K treatment was similar to that in the –K treatment in rhizosphere soil, but the K release in nonrhizosphere soil was greater with the +K than the –K treatment. Overall, the information obtained in this study will be helpful in formulating more precise K fertilizer recommendations for certain soils.     

Electron donor and pH relationships for biologically enhanced dissolution of chlorinated solvent DNAPL in groundwater

Biologically enhanced dissolution offers a method to speed removal of chlorinated solvent dense non-aqueous-phase liquid (DNAPL) sources such as tetrachloroethene (PCE) and trichoroethene (TCE) from aquifers. Bioremediation is accomplished by adding an electron donor to the source zone where fermentation to intermediates leading to acetic acid and hydrogen results. The hydrogen and possibly acetic acid are used by dehalogenating bacteria to convert PCE and TCE to ethene and hydrochloric acid. Reductive dehalogenation is thus an acid forming process, and sufficient alkalinity must be present to maintain a near neutral pH. The bicarbonate alkalinity required to maintain pH above 6.5 is a function of the electron donor: 800 mg/L of bicarbonate alkalinity is sufficient to achieve about 1.2 mM TCE dechlorination with glucose, 1.7 mM with lactate, and a much higher 3.3 mM with formate. Laboratory studies indicate that in mixed culture, formate can be used as an electron donor for complete conversion to ethene, contrary to pure cultures studies indicating it cannot. Various strategies can be used to add electron donor to an aquifer for DNAPL dehalogenation while minimizing pH problems and excessive electron donor usage, including use of injection-extraction wells, dual recirculation wells, and nested injection-extraction wells.     

Biodiversity and ecogeography of wild Lactuca spp. in some European countries

Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.     

Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by Azospirillum brasilense

Inoculation of wheat seedlings with the plant growth-promoting bacterium Azospirillum brasilense Cd was immobilized in alginate microbeads and, without applying any stress, significantly increased the quantity of several photosynthetic pigments, such as chlorophyll a, chlorophyll b, and the auxiliary photoprotective pigments violaxanthin, zeaxanthin, antheroxanthin, lutein, neoxanthin, and β-carotene. This resulted in greener plants with no apparent visible stress. After monitoring the quantity of photosynthetic pigments for 4 weeks, we observed that inoculated plants had higher quantities of pigments in shoot and stem. The greatest difference in the quantity of all pigments between inoculated and noninoculated plants occurred in the first week of growth. Regardless of treatment, the quantity of pigments in stems was three to four times less than the quantity of these pigments in shoots. Application of Azospirillum, either as liquid inoculant or as alginate microbeads, did not alter the positive effect of the bacteria on pigment production or the positive response of the plants towards A. brasilense Cd inoculation.     

Responses of native legume desert trees used for reforestation in the Sonoran Desert to plant growth-promoting microorganisms in screen house

Three slow-growing legume trees used for desert reforestation and urban gardening in the Sonoran Desert of Northwestern Mexico and the Southwestern USA were evaluated whether their growth can be promoted by inoculation with plant growth-promoting bacteria (Azospirillum brasilense and Bacillus pumilus), unidentified arbuscular mycorrhizal (AM) fungi (mainly Glomus sp.), and supplementation with common compost under regular screenhouse cultivation common to these trees in nurseries. Mesquite amargo (Prosopis articulata) and yellow palo verde (Parkinsonia microphylla) had different positive responses to several of the parameters tested while blue palo verde (Parkinsonia florida) did not respond. Survival of all tree species was over 80% and survival of mesquite was almost 100% after 10 months of cultivation. Inoculation with growth-promoting microorganisms induced significant effects on the leaf gas exchange of these trees, measured as transpiration and diffusive resistance, when these trees were cultivated without water restrictions.