共查询到20条相似文献,搜索用时 13 毫秒
1.
Fredrick Kaingu Dandan Liu Lele Wang Jianping Tao Rebecca Waihenya Helen Kutima 《Tropical animal health and production》2017,49(4):823-828
Anticoccidial effects of Aloe secundiflora crude leaf extract was tested in broiler chickens following oral infection with Eimeria tenella. Sixty 22-day-old birds were divided into six groups of ten birds each. Three treatment groups A, B, and C were fed with the extract (100, 250, and 500 mg/day, respectively) mixed in feed for 10 days, and three control groups: group D (drug control) administered 300 mg/l of sulfachloropyrazine sodium soluble powder in drinking water for 5 days, group E (infected/non-medicated positive control), and group F (uninfected/non-medicated negative control). Except for group F, all groups were orally inoculated with 75,000 sporulated oocysts of E. tenella. The effects of the extract on E. tenella infection were evaluated by severity of bloody diarrhea, body weight (BW) gain, oocyst output, and lesion score. No bird in the treated groups died of coccidiosis, and severity of bloody diarrhea was milder than in the positive control group. BW gains in the treated groups were significantly higher than in group E (p < 0.05). The lesion scores of the treated groups were significantly lower than that of group E. Oocyst output in groups A, B, and C were 11.23, 8.24, and 6.82 × 106, respectively. As compared with the negative control group (12.84 × 106), the reductions in oocyst production were 12.54, 35.83, and 46.88%, respectively. Oocyst output significantly reduced with an increase in Aloe dosage. The findings of this study suggest that Aloe secundiflora extract presents an alternative anticoccidial agent for the control of avian coccidiosis. 相似文献
2.
Camacho AT Guitian FJ Pallas E Gestal JJ Olmeda AS Habela MA Telford SR Spielman A 《Tropical animal health and production》2005,37(4):293-302
The control of equine piroplasmosis is becoming increasingly important to maintain the international market open to the horse industry. The purpose of this study was to demonstrate the occurrence of equine piroplasmosis (Theileria equi and Babesia caballi) in Galicia, north-west Spain, and to compare haematological and serum biochemistry parameters between non-parasitaemic horses and horses parasitaemic with T. equi and B. caballi. Sixty serum samples (control group) were taken from healthy horses pastured on two farms, and examined for evidence of equine T. equi and B. caballi infection by indirect fluorescent antibody test (IFAT). Of the 60 samples, 24 (40%) and 17 (28.3%) samples were positive for T. equi and B. caballi, respectively. Twelve (20%) samples were positive for both parasites. Haematology and serum biochemistry were compared between controls and a series of 36 horses clinically affected by T. equi (25) or B. caballi (11). Compared with the healthy group, there was a 43% and 37% decrease in the haematocrit for T. equi and B. caballi infection, respectively. Parasitaemic horses presented an intense anaemia and serum biochemistry signs of liver damage. The anaemia was more severe in T. equi-infected than in B. caballi-infected horses. Our results suggest that equine piroplasmosis is widespread in the region and is a cause for concern. 相似文献
3.
Hao Wang Zihua Li Fu Gao Jiaqing Zhao Mingxing Zhu Xin He Nan Niu Wei Zhao 《Veterinary research communications》2016,40(2):73-79
Objective
This study aims to investigate the immunoprotection of recombinant Eg.P29 (rEg.P29) vaccine and analyze the underlying mechanism in sheep.Methods
Three groups of male sheep were immunized subcutaneously with rEg.P29 and PBS, Freund’s complete adjuvant as controls, respectively. After prime-boost vaccination, the sheep were challenged with encapsulated Echinococcus granulosus eggs. The percentage of protection in sheep was determined 36 weeks after the infection. Humoral immune response was analyzed for specific IgG, IgG1, IgG2, IgM and IgE levels. Moreover, cytokines including interferon (IFN)-γ, interleukin (IL)-2, IL-4,and IL-10 were also evaluated.Results
Immunization with rEg.P29 induced protective immune responses up to 94.5 %, compared with immunoadjuvant group. The levels of specific IgG, IgG1, IgG2, and IgE as well as IFN-γ, IL-2, and IL-4 significantly increased after two immunizations (P < 0.05); however, the levels of IgM and IL-10 did not show difference.Conclusion
rEg.P29 showed Immunoprotection and induced Th1 and Th2 immune responses; hence, rEg.P29 is a potential vaccine for E. granulosus infection.4.
5.
Hossein Nourani Somayeh Matin Abass Nouri Hamidreza Azizi 《Tropical animal health and production》2010,42(6):1225-1227
Bovine sarcocystosis is caused by Sarcocystis cruzi and is known to cause considerable morbidity and mortality in cattle. This species is distributed worldwide in cattle and
is the most prevalent of the Sarcocystis species infecting cattle. There is high infection rate of sarcocyst in cattle in Iran, but to our knowledge, there is no
study about identification of Sarcocystis species. This work aimed to survey prevalence of S. cruzi cyst in slaughtered cattle of Isfahan, Iran. In this study, esophageal and diaphragmatic muscles of 100 cattle were collected
from Fesaran abattoir of Isfahan and examined for the presence of Sarcocystis spp. cysts macroscopically and microscopically. No macroscopic sarcocysts were found in any of the samples. In light microscopy,
89 out of 100 cattle (89%) had thin-walled cysts of S. cruzi, while 21 out of them (21%) had thick-walled sarcocysts. In addition to light microscopy, ultrastructural features of the
thin-walled cyst confirmed the presence of S. cruzi. 相似文献
6.
Liu Q Cai J Zhao Q Shang L Ma R Wang X Li J Hu G Jin H Gao H 《Tropical animal health and production》2011,43(4):741-743
The seroprevalence of Toxoplasma gondii infection in yaks (Bos grunniens) was surveyed in Qinghai Province, northwestern China in May and June 2010. A total of 650 serum samples were collected from
six counties and assayed for T. gondii antibodies by an indirect hemagglutination test. Antibodies to T. gondii were found in 35.08% (228/650) with the highest rate of 55.34% in Chengduo County. The results of the present survey indicated
that infection with T. gondii in cattle is widely spread in China, including yaks in Qinghai Province. 相似文献
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9.
Ozlem Ozmen Mehmet Kale Mehmet Haligur Sibel Yavru 《Tropical animal health and production》2009,41(6):951-958
In this study, pathological, serological and virological examinations were performed on 15 sheep from a flock of 250 sheep
and lambs that suffer from simultaneous naturally occurring BTV, PPRV and SPV outbreaks. SPV was diagnosed macroscopically
and histopathologically, BTV was diagnosed by ELISA, and PPRV was diagnosed pathologically and by ELISA. Clinically fever,
diarrhea, depression, polypnea, conjunctivitis, lacrimation, rhinitis, erosive stomatitis, edema of eyelids, photophobia,
cutaneous eruption with erythematous areas especially noticeable in wool-free parts of the body and axilla lesions evolving
into papules were observed. At necropsy, the most effected organs were lungs and gut. Subepicardial hemorrhages were also
commonly seen. While typical pox lesions were observed in some lambs, usually fibrinous pleuropneumonia was more prominent
lung lesion. SPV and PPRV lesions were seen at the histopathological examination of the lesioned tissues, BT lesions were
mild than SPV and PPRV microscopically. Serum and leukocyte samples of 15 animals were examined for PPRV and BTV by ELISA;
5 samples were positive for PPRV and 6 BTV, 4 were positive for both PPRV and BTV simultaneously. One hundred animals died,
most were lambs. Mortality rates were 100% in lambs and 80% in the herd. 相似文献
10.
Maddur MS Mohan MS Gajendragad MR Gopalakrishna S Singh N 《Veterinary research communications》2008,32(6):481-489
Foot-and-mouth disease (FMD) is one of the most contagious diseases affecting wide range of host species with variable severity and decreased productivity. The present study was undertaken to compare the clinical and leucocytic changes in indigenous Indian cattle and buffaloes experimentally infected with FMD virus (FMDV) Asia 1. A mild type of disease was observed in the cattle, more so in buffaloes infected with FMDV. Difference in terms of type, site and healing of lesion was observed between cattle and buffaloes. Foot lesions were more common than tongue in buffaloes, which were mainly evident in bulb of the heel in contrast to interdigital foot lesions in cattle. Further, FMDV infection induced a transient moderate leucopenia with lymphopenia in both cattle and buffaloes, but monocyte levels diverged. Relationship between the raised body temperature, leucocytic changes and lesion development was observed. Microscopic changes were observed in the keratinized epithelium of tongue and foot. The findings of the present study indicated the need to investigate the early leucocytic changes in cattle and buffaloes in depth for better understanding of the disease process. 相似文献
11.
Ferreira SR de Araújo JV Braga FR Araujo JM Frassy LN Ferreira AS 《Veterinary research communications》2011,35(8):553-558
Ascaris suum is a gastrointestinal nematode parasite of swines. The aim of this study was to observe Pochonia chlamydosporia fungus on biological control of A. suum eggs after fungus passage through swines gastrointestinal tract. Eighteen pigs, previously dewormed, were randomly divided
into three groups: group 1, treated with the fungus isolate VC4; group 2, treated with the fungus isolate VC1 and group 3
did not receive fungus (control). In the treated groups, each animal received a 9 g single dose of mycelium mass containing
P. chlamydosporia (VC1 or VC4). Thereafter, animal fecal samples were collected at the following intervals: 8, 12, 24, 36, 48, 72 and 96 h
after treatment beginning and these were poured in Petri dishes containing 2% water-agar culture medium. Then, 1,000 A. suum eggs were poured into each dish and kept in an incubator at 26°C and in the dark for 30 days. After this period, approximately
100 eggs were removed from each Petri dish and morphologically analyzed under light microscopy following the ovicidal activity
parameters. The higher percentage observed for isolated VC4 eggs destruction was 57.5% (36 h) after fungus administration
and for isolate VC1 this percentage was 45.8% (24 h and 72 h) (p > 0.01). P. chlamydosporia remained viable after passing through the gastrointestinal tract of swines, maintaining its ability of destroying A. suum eggs. 相似文献
12.
M. Sarkar S. Bandyopadhyay G. Krishnan B. S. Prakash 《Tropical animal health and production》2010,42(3):421-424
The changes of glucocorticoid concentrations were measured by highly sensitive enzymeimmunoassay procedures using second antibody
coating technique and cortisol -HRP amplified system for one whole calendar year in yaks. The year was divided into seasons
of cool, intermediate and hot seasons. The glucocorticoid values were lower (P < .01) in hotter months than in cool and intermediate
seasons. Rectal temperature and respiration rates were indicative of heat stress in the hot temperature season and gave significant
negative correlation with circulating corticoid concentration. 相似文献
13.
Amanda Bonalume Cordeiro de Morais Carmen Alicia Daza Bolaños Ana Carolina Alves Cássia Yumi Ikuta Gustavo Henrique Batista Lara Marcos Bryan Heinemann Rogério Giuffrida Fernando Paganini Listoni Mateus de Souza Ribeiro Mioni Rodrigo Garcia Motta Shinji Takai Márcio Garcia Ribeiro 《Tropical animal health and production》2018,50(6):1319-1326
14.
Haematological and serum biochemical values of clinical significance that could serve as reference data for deer kept in captivity
were measured for chital (Axis axis) and barking deer (Muntiacus muntjak). The venous blood from four each of chital and barking deer (n = 8) reared in semi-captivity was collected after proper restraint of the animals. The mean blood haemoglobin, packed cell
volume, total erythrocyte count and total leukocyte count of all the eight deer of the two species were 15.90 ± 0.44,g/dl,
51.44 ± 0.60%, 20.83 ± 0.57 × 106μl and 2.37 ± 0.20 × 103μl. Serum total protein, albumin, bilirubin, cholesterol and blood urea nitrogen irrespective of species were 6.83 ± 0.19,g/dl,
3.90 ± 0.11,g/dl, 0.33 ± 0.08,mg/dl, 106.81 ± 3.59,mg/dl and 24.79 ± 2.11,mg/dl, respectively. Serum enzyme activities indicative
of liver function such as alanine transaminase (ALT) and aspartate transaminase (AST) were 30.38 ± 4.67,units/ml and 42.88
± 5.97,units/ml, respectively. The serum calcium and phosphorus levels of all the eight deer were 10.27 ± 0.36,mg/dl and 8.31
± 0.68,mg/dl, respectively. This is the first report on baseline values in barking deer. The distribution of haematological
and serum biochemical values was fairly normal, suggesting that the mean values could be representative of normal values for
two different deer species. 相似文献
15.
Ansari-Lari M Haghkhah M Bahramy A Novin Baheran AM 《Tropical animal health and production》2009,41(4):553-557
A cross-sectional study was conducted from March to August 2006 in dairy herds in Fars province, southern Iran to determine
the herd-level risk factors for infection with Mycobacterium avium subspecies paratuberculosis (MAP). Statistical analysis using multivariable logistic regression showed that contamination of udders of periparturient
cows with manure (OR = 6.4, P = 0.02) and history of having suspected cases of Johne's disease in the herd (OR = 6.7, P = 0.04)
were significantly associated with the herd infection status. No relationship between breed, herd size and other management
practices with the infection status of the herd were found in this study. Implementing high sanitary measures in the farm,
particularly with respect to manure handling and cleaning could be considered as one of the important aspects in controlling
disease in the region as well as in the future educational effort. 相似文献
16.
Maria Gabriela Xavier Oliveira Leandro Nogueira Pressinotti Giovane Spínola Carvalho Mirela Caroline Vilela Oliveira Luisa Zanolli Moreno Carlos Emilio Cabrera Matajira Alessandro Spínola Bergamo Victor Manuel Aleixo Alexandre Caixeta Veiga Elvis de Souza Corsino Ana Paula Guarnieri Christ Maria Inês Zanolli Sato Andrea Micke Moreno Terezinha Knöbl 《Tropical animal health and production》2017,49(4):777-782
The aim of this study was to perform the identification and molecular characterization of Arcobacter cryaerophilus and Arcobacter butzleri isolated from caiman (Caiman yacare), kept at a production farm, in Brazil. Forty fecal samples were analyzed. After isolation and identification, 21/40 strains of A. butzleri and 19/40 strains of A. cryaerophilus were subjected to PCR for potential virulence gene detection. The results of the PCR showed 38/40 strains positive for the cadF, cj1349, ciaB, and tlyA genes, 39/40 strains positive for the pldA gene, and 40/40 strains positive for the mviN gene. None of the strains presented the irgA gene. Hemagglutinin (hecA gene) and hemolysin (hecB) genes were detected in 21/40 and 16/40 strains, respectively. The SE-AFLP showed a great genetic diversity, but some clonally groups were disseminated in various tanks. These data reveal that the strains presented the same virulence traits described from Arcobacter isolated from food-borne disease in humans. 相似文献
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18.
Benjamin Obukowho Emikpe Mohammed Y. Sabri Stephen O. Akpavie Mohammed Zamri-Saad 《Veterinary research communications》2010,34(7):569-578
Nigerian strain of Peste des Petit Ruminant (PPR) virus and Mannheimia haemolytica (MH) biotype A serotype 2, was used successfully to reproduce a concurrent disease in West African Dwarf goats. The development
of the various pathological features were studied at regular intervals following infection. The acute inflammatory reaction
which had developed by day 3 after initial infection was characterised by flooding of the alveoli by neutrophils, oedema,
hemorrhage and syncytial cells together with a moderate bronchial and bronchiolar epithelial necrosis. This progressed to
a milder acute broncho interstitial pneumonia with giant cells. At this stage, the mucosal immunity were well developed especially
the aggregate form of NALT and more of nodular forms of BALT. The organisms were demonstrated with strong immunostaining in
the necrotic center, necrotic alveolar wall, fibrin, serous exudate, and degenerated leukocyte in the alveoli and respiratory
airways. The bacterial antigens were observed as a strong immunostaining in the blood vessels of the nasal septum, sinusoid
in the liver and interstium of the kidney, cytoplasm of alveolar macrophages, pneumocytes, bronchial and bronchiolar epithelium,
in the monocytes in the blood vessels. These findings confirmed the enhancement of MH tropism especially in the respiratory
tract, liver and kidney. It also showed that West african dwarf goats are highly susceptible to the intratracheal combined
infection of PPR virus and MH. The fact that the infection induces strong mucosal responses, this phenomenon can be explored
in Africa with the use of combined PPR virus and MH intranasal vaccines to curtail the menace of pneumonia associated with
the combined infection on field. 相似文献
19.
Carine Marie-Magdeleine Léticia Liméa Tatiana Etienne Cicero H. O. Lallo Harry Archimède Gisele Alexandre 《Tropical animal health and production》2009,41(7):1531-1538
A study was done to evaluate banana (Musa paradisiaca) as a forage (leaves and pseudo-stems) for feeding Ovin Martinik lambs (OMK), with the aim to test its impact on carcass quality.
Forty four intact OMK male were used after weaning with an initial mean live weight of 14.4 (± 3.3) kg, reared in individual
pens. Animals were offered either Dichantium hay (control diet, Dh) or cut chopped leaves and pseudo-stems of banana (experimental diet, Blps). They were fed 200—250 g.d−1 of commercial concentrate. Lambs were slaughtered according to 3 classes of slaughter weight (SW): SW20, SW23 and SW26. Growth
and carcass performances of both groups were not significantly different, 77 vs. 81 g.d−1 and 42% vs. 43% hot carcass yield, for Dh vs. Blps, respectively. There was a significant (P < 0.05) decrease (31.0 vs. 29.7%)
for the dry matter content of the shoulder for lambs fed the banana diet. However, there was no effect observed for the other
chemical component (CP, lipid and mineral 585, 317 and 95 g.kg−1DM, respectively). The shoulder (20% of the carcass whatever the SW) was precocious as demonstrated by the allometry coefficient
relative to carcass weight (0.894) significantly (P < 0.01) less than 1. It was concluded that, the use of Blps had no significant
effect on growth, carcass weights and yields of the OMK lambs, irrespective of the class of the slaughter weight. From these
initial results, the use of banana foliages and pseudo-stems could be recommended as sources of forages. 相似文献
20.
Thallyta Maria Vieira Leydiana Duarte Fonseca Gabriela Almeida Bastos Viviane de Oliveira Vasconcelos Maria Luíza França Silva Franciellen Morais-Costa Adriano Vinícius de Paiva Ferreira Neide Judith Faria de Oliveira Eduardo Robson Duarte 《Veterinary research communications》2017,41(2):99-106