PCR-SSCP analysis of leptin gene and its association with milk production traits in river buffalo (Bubalus bubalis)

Leptin gene has been found to be associated with various economic traits including milk production and fat quality in dairy animals. In the present study, we investigated genetic variations in intron 1 region of leptin gene in riverine buffaloes (Bubalus bubalis) using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing methods and associated them with milk traits. The study revealed three SSCP variants A, B and C among a total of 301 buffaloes from nine breeds. The frequency of variant C was found invariably high among all the breeds except in Marathwada buffalo. Variant A was found to be absent in Chilika, Nili-Ravi, Nagpuri and Pandharpuri breeds and also had the lowest frequencies in Mehsana, Jaffarabadi, Murrah and Toda breeds. Sequencing of SSCP variants revealed a total of five polymorphic sites, with three haplotypes. Statistical analysis revealed significantly high fat percentage at 150?days in SSCP variant B in Mehsana buffaloes. However, the associations of SSCP variants of leptin gene with total milk yield, 305?days milk yield and total fat yield were found to be non-significant. The present study is the first report on association analysis of leptin gene polymorphisms with milk production and milk quality traits in river buffalo.     

奶牛催乳素基因多态性及其与产奶性状的关联分析

催乳素(PRL)基因是影响奶牛泌乳性状的重要候选基因。通过PCR-RFLP结合测序方法检测了荷斯坦奶牛PRL基因5'侧翼区调控序列的多态性,并利用最小二乘法分析其与泌乳性状的关联性。结果显示:在PRL基因5'侧翼序列的906位点处发现A/G突变。共发现AA、AG和GG三种基因型,基因型频率分别为0.239 3、0.522 8和0.177 9,A和G的等位基因频率为0.530 7和0.469 3。多态信息含量检测结果显示该位点处于中度多态,卡方检验显示该位点处于Hardy-Weinberg不平衡状态(P0.05)。关联分析结果表明,该位点多态对产奶量、乳脂率均有影响。     

肌联蛋白基因SNPs位点多态性与延边黄牛生长性状间的关联分析

为了研究延边黄牛肌联蛋白(Titin,TTN)基因的多态性对延边黄牛生长性状的影响,选取208头健康的32月龄延边黄牛,利用PCRRFLP和PCR-SSCP方法进行TTN基因遗传变异检测。结果表明:延边黄牛TTN基因存在2个突变位点,分别为A47751G突变,导致氨基酸Ile→Val的错义突变,C73301A同义突变;χ2检验显示,2个突变位点在所检测延边黄牛群体中处于Hard-Weinberg平衡状态(P005);关联分析显示,延边黄牛TTN基因2个突变位点的不同基因型与体高、体斜长、胸围和尻长上差异显著(P005)。将2个突变位点合并基因型分析结果表明,组合AACC基因型个体在体高和体斜长显著高于AATT型个体(P005),初步认为TTN基因对延边黄牛生长性状的有显著影响,其中组合AACC可能是影响延边黄牛生长性状的最佳基因型组合。     

Evaluation of association between polymorphism within the thyroglobulin gene and milk production traits in dairy cattle

In dairy cattle, many studies have reported quantitative trait loci (QTL) on the centromeric end of chromosome 14 that affect milk production traits. One of the candidate genes in this QTL region – thyroglobulin (TG) – was previously found to be significantly associated with marbling in beef cattle. Thus, based on QTL studies in dairy cattle and because of possible effects of this gene on fat metabolism, we investigated the association of TG with milk yield and composition in Holstein dairy cattle. A total of 1279 bulls from the Cooperative Dairy DNA Repository Holstein population were genotyped for a single nucleotide polymorphism in TG used previously in beef cattle studies. Analysis of 29 sire families showed no significant association between TG variants and milk production traits. Within‐sire family analysis suggests that TG is neither the responsible gene nor a genetic marker in association with milk production traits.     

Polymorphism of lactoferrin gene in Egyptian goats and its association with milk composition traits in Zaraibi breed

Tropical Animal Health and Production - The objectives of this study were to identify polymorphisms in the lactoferrin gene among three Egyptian goat breeds (Barki, Zaraibi, and Damascus) and to...     

湖羊和小尾寒羊骨形成蛋白受体IB型基因多态性与泌乳性状的相关性分析

为探究骨骼形成蛋白IB型受体基因(BMPR-IB)与泌乳性状的相关性,采用PCR-RFLP方法检测BMPR-IB在湖羊(41只)和小尾寒羊(30只)群体中的多态性,并分析了其泌乳期1~56 d的泌乳性状。结果显示:在湖羊群体中发现BB和B+两种基因型,基因频率为0.965和0.035;与泌乳性状结合分析:BB基因型乳蛋白显著高于B+型个体(P0.05)。小尾寒羊群体中发现BB、B+和++3种基因型,频率分别为0.27,0.4和0.33;++型个体泌乳量极显著高于BB型个体(P0.01),B+型个体泌乳量显著高于BB型个体(P0.05);从群体泌乳量来看为++型B+型BB型;++型个体乳糖显著高于BB型个体(P0.05)。     

澳系进口荷斯坦奶牛CXCR1基因遗传多态性与泌乳性状及体细胞评分的关联性分析

采用聚合酶链式反应-单链构象多态性(PCR-SSCP)技术对以海丰奶牛场588头澳系进口荷斯坦牛牛趋化因子受体1(Chemokine receptor 1,CXCR1)基因的遗传多态性进行分析;采用混合动物模型分析CXCR1基因2个突变位点与测定日产奶量、乳脂率、乳蛋白率、305d产奶量、305d乳脂量、305d乳蛋白量及体细胞评分7个性状的相关性,寻找可用于生产实际的分子标记。结果显示,CXCR1基因5′侧翼区-1830位点发生了A→G的突变,检测到AA、AG和GG 3种基因型,频率分别为0.684、0.289和0.027,等位基因A和G的频率分别为0.828和0.172;GG基因型奶牛的日产奶量、SCS和305d产奶量均极显著高于AA基因型(P<0.01),而其乳脂率和乳蛋白率却显著低于AA基因型奶牛(P<0.05)。编码区783位点仅发现AA和AC 2种基因型,基因型频率分别为0.886和0.114,等位基因A和C的频率分别为0.943和0.057。AA型的个体的日产奶量、305d脂肪产量和305d蛋白产量极显著高于AC型个体,而其乳脂率、乳蛋白率和SCS却极显著低于AC型个体(P<0.01)。结果表明,CXCR1基因遗传突变对澳系进口荷斯坦牛泌乳性状和乳房炎抗性有较大的遗传效应,可用于澳系进口荷斯坦牛的分子标记辅助选择。     

澳系进口荷斯坦奶牛CXCR1基因遗传多态性与泌乳性状及体细胞评分的关联性分析

采用聚合酶链式反应-单链构象多态性（PCR—SSCP）技术对以海丰奶牛场588头澳系进口荷斯坦牛牛趋化因子受体1（Chemokine receptor1,CXCR1）基因的遗传多态性进行分析;采用混合动物模型分析CXCRl基因2个突变位点与测定日产奶量、乳脂率、乳蛋白率、305d产奶量、305d乳脂量、305d乳蛋白量及体细胞评分7个性状的相关性,寻找可用于生产实际的分子标记。结果显示,CXCR1基因5’侧翼区-1830位点发生了A—G的突变,检测到AA、AG和GG3种基因型,频率分别为0．684、0．289和0．027,等位基因A和G的频率分别为0．828和0．172;GG基因型奶牛的日产奶量、SCS和305d产奶量均板显著高于AA基因型（P〈0．01）,而其乳脂率和乳蛋白率却显著低于AA基因型奶牛（P〈0．05）。编码区783位点仅发现AA和AC2种基因型,基因型频率分别为0．886和0．114,等住基因A和c的频率分别为0．943和0．057。AA型的个体的日产奶量、305d脂肪产量和305d蛋白产量极显著高于AC型个体,而其乳脂率、乳蛋白率和SCS却极显著低于AC型个体（P〈O．01）。结果表明,CXCR1基因遗传突变对澳系进口荷斯坦牛泌乳性状和乳房炎抗性有较大的遗传效应,可用于澳系进口荷斯坦牛的分子标记辅助选择。     

Polymorphism association and expression analysis of alpha-lactalbumin (LALBA) gene during lactation in Nili Ravi buffalo

Tropical Animal Health and Production - Alpha-lactalbumin has been reported as a highly polymorphic gene that potentially alters the gene expression and is associated with milk composition in dairy...     

鸡钙蛋白酶抑制蛋白基因多态性及其与肉质性状的相关性

为探讨钙蛋白酶抑制蛋白(Calpastatin,CAST)基因作为影响鸡肉质性状候选基因的可能性,本研究以8个优质鸡新品系为试验材料,采用PCR-SSCP方法对CAST基因的部分编码区进行单核苷酸多态性(SNPs)检测。结果发现了CAST基因1个多态性位点(G→T),使试验鸡群表现出AA、BB和AB这3种基因型。利用SAS(8.01)软件统计分析了3种基因型与优质鸡肉质性状的相关性,结果表明,CAST基因的多态性对鸡肌内脂肪含量、肌纤维密度和直径有显著的影响(P0.05),对其他肉质性状无显著影响(P0.05);推测CAST基因可能对鸡肉质性状具有很大的影响或与控制肉质性状的主基因连锁。     

Polymorphism in GHRH gene and its association with growth traits in Chinese native cattle

Growth hormone-releasing hormone (GHRH) is secreted by the hypothalamus and stimulates growth hormone (GH) released from the pituitary. Mutations detected in GHRH gene showed associations with animal production traits. The purpose of this study was to investigate the association of the GHRH gene with growth traits in Chinese native cattle. PCR-SSCP and sequencing were used to detect mutations of the GHRH gene in this study. One novel mutation 4251nt (C>T) was found and the frequencies of C allele were 0.8778 and 0.8476 for Qinchuan and Nanyang cattle, respectively. Body weight with the CT genotype was significantly higher (P<0.05 or P<0.01) than those with CC genotype for different growth periods (6, 12, 18, and 24 months old) in Nanyang cattle. Our findings suggested that polymorphism in bovine GHRH might be one of the important genetic factors to influence body weight.     

Bioinformatics analysis of candidate genes for milk production traits in water buffalo (Bubalus bubalis)

Tropical Animal Health and Production - Water buffalo (Bubalus bubalis) is an important livestock species in developing countries due to its contribution to meat, milk production, and a certain...     

鸽载脂蛋白B基因多态性与生长性状相关性分析

利用PCR-SSCP技术和DNA测序方法检测两群体鸽apoB基因编码区序列的单核苷酸多态性,最小二乘分析表明,A57G突变位点导致的3种基因型对石岐肉鸽屠体率及灰色肉鸽屠体重、腹脂率、肝脏重有显著影响(P<0.05);对灰色肉鸽活重、腹脂重、肝脏率及石岐肉鸽活重和全净膛率有一定影响(P<0.2).多重比较显示,石岐肉鸽群体中,BB基因型个体的屠体率高于从基因型个体.灰色肉鸽群体中,BB基因型个体的屠体重、肝脏重、腹脂率均为最大.