Pilot plant for processing flax fiber

A flax fiber pilot plant is needed to process small samples of flax straw into fibers to facilitate research on retting and fiber properties. Our objective was to develop and test a modular design for a flax processing pilot plant based on a commercial line that was capable of cleaning fiber and seed flax straw from unretted, dew-retted, and enzyme-retted samples. The USDA Flax Fiber Pilot Plant (Flax-PP), which is the only research facility of this type in the United States, was designed according to the commercial ‘Unified Line’ (Czech Flax Machinery), but smaller and constructed in four individual modules. The modules and their order for processing were as follows: 9-roller calender, top shaker, scutching wheel, top shaker, 5-roller calender, and top shaker. Illustrations and diagrams of the operating modules are presented. Unretted ‘Neche’ linseed flax, dew-retted ‘Natasja’, and enzyme-retted ‘Jordan’ fiber flax were processed, and the cumulative weight loss of material at successive processing steps was determined to assess the effectiveness of cleaning. Fiber strength, fineness, and elongation were determined for the retted samples after cleaning through all the steps in the Flax-PP. A yield of fine fiber from the retted stems processed through the Flax-PP was acquired from further cleaning and refining by passage through a Shirley Analyzer. The various samples behaved differently at different stages of processing and the resulting fibers had different properties. The dew-retted Natasja fibers were stronger and finer than the enzyme-retted Jordan flax after pilot plant processing, but the Jordan fibers appeared cleaner and better retted. The Flax-PP effectively processed samples of diverse characteristics and will facilitate integrated research on retting methods for fibers with tailored properties.     

Flax-cotton fiber blends: Miniature spinning,gin processing,and dust potential

Development of a flax (Linum usitatissimum L.) industry in North America is desired to supply a domestic source of clean, consistent quality textile fiber for blending with cotton. The objective of this work was to evaluate portions of traditional cotton gin equipment (extractor feeder and lint cleaner) and the “50-g cotton-spinning test (CST)” for flax. Dust was collected on an area sampler in an isolated card room to evaluate dust potential during textile pilot plant processing. Fibers retted by diverse means were cleaned on two separate portions of Continental Eagle's pilot plant cotton gin stand, the Super 96 Feeder and the 24D lint cleaner. Fibers separated and removed from flax stalks by these gin sections were compared against the standard ‘unified line’ processing technique of the USDA Flax Pilot Plant. Test yarns were then made in a CST with cotton and flax blends to provide an indirect measurement of fiber properties that can be related to the retting and gin cleaning processes. The yarns were tested for strength and evenness. Flax fibers that displayed the most favorable properties in the CST were then spun in 23 kg lots in the pilot plant at the following cotton/flax blend ratios: 100/0, 75/25, 50/50, 25/75, and 20/80. With modifications, it appears that portions of a cotton gin stand are able to process adequately small samples of properly retted flax stalks. The CST with minor adjustments provides useful data for ranking and further large-scale flax processing. As expected, it appears that flax fiber can be successfully cleaned on a cotton processing line and that increasing the amount of flax generates additional dust.     

Fiber yield and quality of fiber nettle (Urtica dioica L.) cultivated in Italy

To evaluate the production potential of fiber nettle crops in Tuscany (Italy), a German clone of fiber nettle was cultivated during 2006–2007.Although a longer experimentation is essential, the two first years of trials showed that the German clone used also seems to give good results in term of growth and fiber yield in an environment like central Italy, with higher temperatures and generally lower rainfall. Indeed the stalk mean dry matter obtained was about 15.4 Mg ha^?1 with a mean fiber content of about 11% of stalk dry matter, and the resulting fiber yield was 1696 kg ha^?1, comparable to or higher than those reported in the literature.The differences in chemical, physical and mechanical characteristics of fibers extracted from different portions of stalks seemed to indicate an intrinsic heterogeneity of the fibers along the stem. Fiber mean diameter values ranged from 47 to 19 μm and fiber length from 43 to 58 mm moving from stalk bottom to top. Tensile strength of the bottom part of the stalk was much lower than that of the other parts, with mean values of about 24 and 60 cN tex^?1, respectively. More constant mean values along the stalk were found for the elongation parameter (2.3–2.6%). Lignin content decreased moving toward the stalk top from about 4.4% to 3.5%.These physical–mechanical characteristics confirmed the potential of the fibers of nettle cultivated in Tuscany to be used for textile purposes. Indeed they were similar to hemp fibers in diameter, lignin content and elongation, and similar to flax or cotton in tensile strength.     

Oil Red as a histochemical stain for natural fibers and plant cuticle

Properties of natural fibers are influenced by the nature of their surface. Oil Red was evaluated as a histochemical stain for the waxy components on the surface of cotton and flax fibers and of plant cuticles. A positive reaction for arachidyl stearate and differential staining of fibers after sequential extraction of fatty acids and alcohols indicated that Oil Red stained wax components in plant materials. For cotton (Gossypium hirsutum) fibers, Oil Red stained to a greater extent the regions closest to the seed coat, especially at points where fibers attached to the seed coat. Fiber regions at a distance from the seed coats stained irregularly, suggesting that the wax was unevenly distributed. Flax (Linum usitatissimum) bast fibers, in contrast, did not stain with Oil Red, but the protective stem cuticle was intensely stained. The positive histochemical reaction for cuticle identified non-fiber fragments in processed and cleaned flax fibers, thus providing a quick method to detect visually trash components in fiber and products. Likewise, bast fibers from kenaf (Hibiscus cannabinus) did not stain well with Oil Red, whereas the stem cuticle gave a positive reaction. The general usefulness of Oil Red as a histochemical stain for the plant cuticle was demonstrated in leaves and stems of mature corn (Zea mays) and fresh bermudagrass (Cynodon dactylon) leaves. Oil Red provides a quick, qualitative histochemical method to demonstrate the wax-containing cuticle in plants.     

Improving the use of kenaf for kraft pulping by using mixtures of bast and core fibers

Kenaf (Hibiscus cannabinus L.) is a herbaceous annual plant amenable to use as a papermaking raw material. Kraft and soda pulping of kenaf have so far been done exclusively on the bark fraction (about 34–38% of the stem) or whole stem of the plant. Using kenaf bark exploits the higher quality of its bast fibers but reduces the typically high crop yields of this plant. In any case, core kraft pulp has acceptable properties some of which (e.g. tensile index, burst index) can even surpass those of bark pulp. Pulp made from both fractions has been found to exhibit better bonding properties than bark pulp. However, too high a proportion of core fibers can result in difficult drainage, a low tear strength or poor air permeability. These problems restrict the proportion of core that can be mixed with bast fibers, hinders separation of the two fractions and raises operational costs.The primary purpose of this study was to examine the influence of the core–bark ratio on the properties of mixed kenaf pulp. We used unrefined core pulp and refined bark pulp. Based on the results for kraft sacks, obtaining kenaf paper from both fractions has some advantages. Because Gurley air porosity changed dramatically with the proportion of core pulp used, it was used to determine the maximum amount of core fibers to be added to bast fibers. A proportion of up to 34% was found to have no adverse effect on air permeability. Such a proportion allowed paper strength to be preserved with an acceptable tear index (19.8 mN m²/g) and excellent tensile index (72 N m/g). Also, energy consumption was reduced if only the bark fraction was refined. The proposed strategy thus provides increased fiber yields of kenaf per hectare per year and valorizes the core fraction.     

Bio-accumulation and distribution of heavy metals in fibre crops (flax,cotton and hemp)

Flax, hemp and cotton, grown in industrially polluted region, were included in the present research. The experimental plots were situated at different distances (0.5 and 15 km) from the source of pollution—the Non-Ferrous-Metal Works (MFMW) near Plovdiv. We investigated the level of pollution and the way heavy metals enter the fibre crops, by taking soil and plant samples. The contents of heavy metals in plant materials (roots, stems, leaves, seeds, flowers) were determined after the method of the dry mineralization. The quantitative measurements were carried out with inductively-coupled plasma (ICP).A clearly distinguished species peculiarity exists in the accumulation of heavy metals in the vegetative and reproductive organs of flax, hemp and cotton. Flax is the crop that most strongly absorbs and accumulates heavy metals from the soil, followed by hemp and cotton. The distribution of the heavy metals along the plant axis of the studied crops seems to be selective, therefore their contents in flax and hemp are decreasing in the following order: roots>stems>leaves>seeds, while in cotton: leaves>seeds>roots>stems. A strongly exhibited tendency towards decrease of the contents of heavy metals in the fibre crops is observed as the distance from the NFMW increases.Flax and hemp are cultures, suitable for growing in industrially polluted regions—they remove considerable quantities of heavy metals from the soil with their root system and can be used as potential crops for cleaning the soil from heavy metals.     

Population density and row spacing effects on dry matter yield and bark content of kenaf (Hibiscus cannabinus L.)

Efforts to bring kenaf from experimental crop status to an accepted alternative in established cropping systems have been ongoing for years. To compete with existing crops and maximize monetary returns, the effects of agronomic practices on yield and crop quality must be better understood. Row spacing and population density are implicated in dry matter yield, and therefore, bast fiber production. Four row spacings, four population densities, and two cultivars were examined to determine these effects on dry matter accumulation and bark content. Of all factors evaluated, only row spacing significantly affected dry matter yield. Yield was greatest at the 35.5-cm row spacing, and lowest at the 71- and 101.6-cm row spacings. With regard to bast yield, calculated as a percentage of total sample dry weight, a significant cultivar × row spacing interaction occurred. However, data indicate that manipulating row spacing to maximize total stalk yield per hectare resulted in the highest bast fiber yield for the two cultivars tested. The narrowest row spacing of 35.5 cm, gave the greatest biomass yield as well as the highest bark yield per hectare.     

Evaluation of harvesting time effects on kenaf bast lignin by pyrolysis-gas chromatography

A Chinese kenaf (Hibiscus cannabinus L.), variety Sekko-ichi, was planted in May 1996, and harvested at 76, 116, 152, 185, and 226 days after planting. The hand-separated bast fibers were analyzed for lignin content and in situ lignin composition to clarify their variations with maturity. Bast fibers increased in lignin until 152 days after planting, and did not increase thereafter. Variations in in situ lignin compositions with maturity were monitored using pyrolysis-gas chromatography. The volatile pyrolysis products released at 500 °C for 4 s were identified by gas chromatography-mass spectrometry. Pyrolysis products derived from guaiacyl and syringyl lignin units were revealed. The yield of guaiacyl lignin-derived pyrolysis products decreased with maturity, while the yield of the syringyl analogous increased with maturity. The ratio of yield of syringyl lignin-derived products to that of guaiacyl ones (S/G) increased from 1.86 to 3.16 with maturity, but remained constant at 152 days after planting. High S/G ratio showed that mature bast lignin is of syringyl type. Quantitative pyrolysis results suggested that harvesting of kenaf at age of 5-months growth period provides a raw material with an ease of delignification.     

Residual effect of poultry litter applied to cotton in conservation tillage systems on succeeding rye and corn

The burgeoning poultry industry in the southeastern US is presenting a major environmental problem of safe disposal of poultry litter (PL). In a comprehensive study, we explored ways of PL use in conservation tillage-based cotton (Gossypium hirsutum L.) production systems on a Decatur silt loam soil in north Alabama, from 1996 to 1999. The study reported here-in presents the residual effects of PL applied to cotton in mulch-till (MT) and no-till (NT) conservation tillage systems in 1997 and 1998 cropping seasons on N uptake, growth, and yield of rye (Secale cereale, L.) cover crop and rotational corn (Zea mays L.) in 1999. Rye was grown without additional N, whereas corn was grown at three inorganic N levels (0, 100, and 200 kg N ha⁻¹). Poultry litter was applied to cotton in 1997 and 1998 at 0, 100, and 200 kg N ha⁻¹. Residual N from PL applied to cotton in 1997 and 1998 produced up to 2.0 and 17.3 Mg ha⁻¹, respectively, of rye cover crop and corn biomass (includes 7.1 Mg ha⁻¹ of corn grain yield) without additional fertilizer. Therefore, in addition to supplying crop residues which reduce soil erosion, increase soil organic matter, and conserve soil moisture, the rye cover crop was able to scavenge residual N left by the cotton crop, which would otherwise, be at risk of being leached and pollute groundwater resources. Poultry litter applied to cotton also increased corn grain quality as shown by up to 100% increase in grain N content compared to the 0N treatment. Using PL with a slower rate of N release compared to inorganic fertilizer to meet some of the N requirements of corn, will not only reduce N fertilizer costs for corn, but will also reduce the risk of nitrate N leaching into groundwater. The maximum amount of crop residues added to the cotton based cropping system by residual N from PL and inorganic N was 21.3 Mg ha⁻¹. This will lead to an increase in soil organic carbon and soil structure in the long term and a reduction in soil erosion, thereby further improving soil productivity, while at the same time, protecting the environment from nitrate pollution and soil degradation. Our study demonstrates that cotton under conservation tillage system in combination with rye cover crop and rotational corn cropping could use large quantities of PL thereby avoiding serious potential environmental hazards.     

Evaluation of flax accessions for high value textile end uses

Due to recent changes in EC subsidies for flax cultivation it has been difficult to grow short fibre flax profitably in the UK. The Texflax project aimed to demonstrate that high quality flax fibre can be produced and processed on short fibre cotton spinning systems. Initially 92 flax accessions were cultivated on test sites in the UK over three growing seasons to explore the range of fibre diameter found in fibre flax. The efficacy of applying a translocating herbicide at different stages of plant maturity for optimum fine fibre production was explored. A range of factors indicated that application at the midpoint of flowering stage is favourable for the desiccation of flax and onset of retting. Fibre was caustic extracted using a laboratory method developed at De Montfort University, and fibre evaluated in terms of diameter, length, consistency and cleanliness. At the end of the project five accessions from the original 92 were chosen as producing optimal quality fibre suitable for high value textile end uses. Improved agronomy and subsequent processing enabled yarns with a 50:50 cotton:flax blend to be spun at 26 N m yarn count, the normal blend ratio for this count being 70:30. The yarn properties show an improvement when compared to standard products and finer quality fabrics have been prepared using the yarns.     

Effects of plant growth regulator treatments on stem vascular tissue development in linseed (Linum usitatissimum L.)

Linseed (Linum usitatissimum L.) is a widely grown source of industrial and edible oil. Other varieties of the same species (flax) are cultivated for the long, strong bast fibres of their stems. The bast fibres of linseed generally go unused, although there is growing interest in developing linseed into a dual-purpose flax from which both seed and fibre could be utilized. Towards this objective, an improved understanding is required of the role of plant growth regulators in stem and fibre development in linseed. We have tested the effects of applying varying combinations of gibberellic acid (GA₃), the auxin indole-3-acetic acid, and a GA biosynthesis inhibitor (paclobutrazol) to an elite linseed variety (CDC Bethune). Results showed that GA stimulated stem elongation, stem expansion and the proliferation, expansion, elongation and cell wall thickening of xylem fibres. The impact of GA on phloem tissues was less apparent, although GA had a positive effect on the number of bast fibres observed in stem transverse section, and GA₃ application in combination with IAA increased the thickness of bast fibre secondary walls nearly two-fold. Other than the bast fibre cell walls, IAA treatments (alone or in combination with GA₃) did not affect most aspects of linseed stem development, suggesting that the observed effects of GA were not mediated by cross-talk with IAA. The relationships defined here between GA, stem architecture, and bast fibre properties in linseed provide a useful framework for manipulation of fibre properties through breeding, biotechnology, and field treatments.     

Modeling chickpea growth and development: Nitrogen accumulation and use

Quantitative information regarding nitrogen (N) accumulation and its distribution to leaves, stems and grains under varying environmental and growth conditions are limited for chickpea (Cicer arietinum L.). The information is required for the development of crop growth models and also for assessment of the contribution of chickpea to N balances in cropping systems. Accordingly, these processes were quantified in chickpea under different environmental and growth conditions (still without water or N deficit) using four field experiments and 1325 N measurements. N concentration ([N]) in green leaves was 50 mg g⁻¹ up to beginning of seed growth, and then it declined linearly to 30 mg g⁻¹ at the end of seed growth phase. [N] in senesced leaves was 12 mg g⁻¹. Stem [N] decreased from 30 mg g⁻¹ early in the season to 8 mg g⁻¹ in senesced stems at maturity. Pod [N] was constant (35 mg g⁻¹), but grain [N] decreased from 60 mg g⁻¹ early in seed growth to 43 mg g⁻¹ at maturity. Total N accumulation ranged between 9 and 30 g m⁻². N accumulation was closely linked to biomass accumulation until maturity. N accumulation efficiency (N accumulation relative to biomass accumulation) was 0.033 g g⁻¹ where total biomass was <218 g m⁻² and during early growth period, but it decreased to 0.0176 g g⁻¹ during the later growth period when total biomass was >218 g m⁻². During vegetative growth (up to first-pod), 58% of N was partitioned to leaves and 42% to stems. Depending on growth conditions, 37–72% of leaf N and 12–56% of stem N was remobilized to the grains. The parameter estimates and functions obtained in this study can be used in chickpea simulation models to simulate N accumulation and distribution.     

Studies on Hibiscus cannabinus and Hibiscus sabdariffa as an alternative pulp blend for softwood: An optimization of kraft delignification process

Hibiscus cannabinus and Hibiscus sabdariffa, agro-based residues consist of bast and wood fibers which resemble to those of softwood and hardwood, respectively. The runkel ratio of core fibers H. cannabinus and H. sabdariffa is comparable to that of Picca abies, whereas, it is much less than those of hardwood like Eucalyptus tereticornis. The slenderness ratio of H. cannabinus is much closer to P. abies in comparison to H. sabdariffa whereas, it is 7.7 and 13% less than E. tereticornis. The flexibility coefficient of H. cannabinus and H. sabdariffa are slightly lower than that of P. abies but it is 59.6 and 57.0% are more than that of E. tereticornis. It indicates that morphological characteristics of core fibers of H. cannabinus and H. sabdariffa closely resemble to that of softwood except fiber length which can be compensated by long bast fibers. Due to identical pulping conditions, H. cannabinus and H. sabdariffa can be delignified together by kraft pulping process. The optimum cooking conditions for H. cannabinus and H. sabdariffa were found to be as, active alkali 16%, sulfidity 20%, temperature 160 °C, time (at temperature) 120 min and wood to liquor ratio of 1:4.5. An anthraquinone (AQ) dose of 0.05% at an active alkali dose of 13% (as Na₂O) produces the screening rejects and kappa number similar to that obtained by using 15% active alkali (as Na₂O). The reaction kinetics study indicates that delignification is of first order. Low sulfidity AQ additive kraft pulping at constant H-factor produces better strength properties compared to non-additive kraft cooks.     

Evaluation of the fineness of degummed bast fibers

Fiber fineness characteristics are important for yarn production and quality. In this paper, degummed bast fibers such as hemp, flax and ramie have been examined with the Optical Fiber Diameter Analyzer (OFDA100 and OFDA2000) systems for fiber fineness, in comparison with the conventional image analysis and the Wira airflow tester. The correlation between the results from these measurements was analysed. The results indicate that there is a significant linear co-relation between the fiber fineness measurement results obtained from those different systems. In addition, the mean fiber width and its coefficient of variation obtained from the OFDA100 system are smaller than those obtained from the OFDA2000 system, due to the difference in sample preparation methods. The OFDA2000 system can also measure the fiber fineness profile along the bast fiber plants, which can be useful for plant breeding.     

Effect of growth regulators on yield and fiber quality in ramie (Boemheria nivea (L.) Gaud.), China grass

The effects of two mixtures of four plant growth regulators (choline chloride, gibberellin (GA₃), benzyladenine (6-BA) and NaHSO₃) at 20:9:5:800 mg kg⁻¹ (H1) and 20:42:43:2350 mg kg⁻¹ (H3) (active ingredients), respectively, were investigated on yield and fiber quality in ramie (Boehmeria nivea (L.) Gaud.). The mixtures were sprayed over the canopy at two growth stages (10 and 20 days after the previous cut) of field-grown ramie. The treatments increased raw fiber yield by 13–18%, and improved fiber fineness by 57–349 m g⁻¹, increased number of leaves per plant, and also improved all yield components. Treatment H1 resulted in a denser distribution, smaller diameters and greater quantity of fiber cells in stem cross-section. Physiological responses included improving leaf water status, increasing net photosynthetic rate, and decreasing electrolyte exosmosis rate.     

Will nutrient-efficient genotypes mine the soil? Effects of genetic differences in root architecture in common bean (Phaseolus vulgaris L.) on soil phosphorus depletion in a low-input agro-ecosystem in Central America

Crop genotypes with root traits permitting increased nutrient acquisition would increase yields in low fertility soils but have uncertain effects on soil fertility in the long term because of competing effects on nutrient removal vs. the soil conserving effects of greater crop biomass. This study evaluated the relative importance of phosphorus loss in crop extraction vs. phosphorus loss in soil erosion as influenced by genetic differences in root shallowness and therefore phosphorus uptake in common bean (Phaseolus vulgaris L.). Six recombinant inbred lines of varying root architecture and two commercial genotypes of bean were grown in unfertilized, steeply sloped (32%), low phosphorus (5.8 mg kg^?1, Fe-strip) Udults in Costa Rica. Fertilized (60 kg total phosphorus ha^?1) plots of commercial genotypes were also included in the study. Runoff was monitored throughout the bean growing season in 2005 and 2006, and in 2006, monitoring continued through the maize growing season. Phosphorus removed in plant biomass at harvest through the 2006 bean–maize crop cycle averaged 7.3 kg ha^?1 year^?1, greatly exceeding phosphorus loss due to erosion (0.15–0.53 kg ha^?1 year^?1) in unfertilized plots. In fertilized bean plots, total biomass phosphorus averaged 6.32 kg ha^?1 year^?1 and total eroded phosphorus averaged 0.038 kg ha^?1 year^?1, indicating rapid sorption of fertilizer phosphorus. Shoot growth of several recombinant inbred lines under low phosphorus was comparable to that of fertilized commercial genotypes, illustrating the effectiveness of selection for root traits for improving plant growth in low-phosphorus soils. Genotypic differences in root architecture of recombinant inbred lines led to 20–50% variation in groundcover by shoots, which was associated with 50–80% reduction in sediment loss. This study demonstrates that root architecture traits can affect nutrient cycling at the agro-ecosystem level, and that integrated nutrient management strategies are necessary to avoid soil nutrient depletion.     

Evaluating chemical indices of guayule rubber content: Guayulins A and B

Guayule (Parthenium argentatum Gray) is now a commercial crop for the production of high-quality, hypoallergenic natural rubber latex. Because guayule is relatively resistant to both insect and disease pests, its cultivation requires little chemical input. It has been postulated that guayule's chemical defense system is based on terpene derivatives such as guayulins A and B. The goals of this research were to: (1) describe the distribution of guayulins A and B throughout the plant, (2) determine whether guayulins can be used as a predictor of rubber content/yield (is guayulin content related to rubber content), and (3) determine whether the guayulin content/distribution has been changed through plant breeding.Two plants each of three different lines (11591, AZ-1, and AZ-3) and two ages (1 and 2 years old) were harvested at the soil line in the fall of 2002 and spring of 2003. Plants were separated into eight parts: brown leaves, green leaves, stem tips, stems less than 5 mm in diameter, stems between 5 and 10 mm, stems greater than 10 mm, green stems, and flower parts. Samples were analyzed for guayulins A and B and rubber content.Guayulins A and B and rubber were found in all plant parts, but were most prevalent in stems larger than 10 mm in diameter, and were significantly correlated with each other in these stems. Guayulin A was found in greater concentrations than guayulin B. There were no significant differences between plant ages for rubber, or guayulin content at the time of harvest, or guayulins for harvest season. Rubber percent was higher in spring, which agrees with numerous other investigations.Guayulin A in the stems is correlated with the total rubber in the plant, suggesting it as a potential selection tool. However, rubber in the same plant parts has a higher correlation with total plant rubber and remains a better and easier selection criterion than guayulin A.The extent to which guayulin contents differed between older, relatively unimproved lines and newer improved lines was evaluated. Variety 11591 had a higher concentration of guayulin A than guayulin B, so that the ratio of A to B was much higher than in the two improved lines (AZ-1 and AZ-3). It appears that the ratio of guayulin A to guayulin B has been changed in the two newer selections, but it is unclear whether this will affect pest resistance.     

Stover quality of dual-purpose sorghums: genetic and environmental sources of variation

Improvement of the nutritive value of dual-purpose sorghum (Sorghum bicolor (L.) Moench) stover is an important objective for the semi-arid tropics where sorghum crop residue is extensively used for livestock feed. To identify the relative importance of genetic and environmental sources of variation for nutritive value, leaves and stems of six diverse dual-purpose sorghum cultivars were evaluated for in vitro gas production (Gas48hr), neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, nitrogen, and ash contents under two fertility and two plant-density regimes during 2 years in India. Substantial genotypic differences were observed for stem Gas48hr (25.7 to 33.0 ml in 200 g⁻¹ dry matter (DM)) and NDF (564–687) content. Gas48hr and NDF content of stems exhibited more promise as selection criteria than those of leaves, as stems showed larger portion of variation attributed to genotypes, relatively less genotype by environment (GE) interactions, and were closely related to whole-plant values. Year, nitrogen fertilization and plant density showed very little influence on Gas48hr, NDF or ADF of leaves and stems. Gas48hr exhibited substantial GE interactions with all environmental factors, indicating the need for multi-environment testing to achieve progress.     

Kenaf seed storage duration on germination,emergence, and yield

Kenaf (Hibiscus cannabinus L.) is a potential alternative crop being developed for fiber production. Because planting area varies dramatically from year to year, seed supplies may greatly exceed use so that the excess seed must be stored for one to several years. The objectives of this study were to determine the effect of seed storage duration at 10 °C on germination, vigor, emergence, and yield. Replicated trials were established at Starkville, MS in 1999 and 2000 to evaluate field emergence and biomass yield of kenaf seed from five ‘Everglades 41’ (‘E41’) harvest year seed lots stored at 10 °C in ambient relative humidity for up to 4 years. Germination of these same seed lots under standard (20–30 °C) and cool (20 °C) temperatures, and seed vigor was evaluated over time. Field emergence was the same for the different seed storage durations up to 4 years, but was directly affected by drought conditions for each planted year. Biomass yields ranged from 12.39 to 14.57 Mg ha⁻¹ in 1999 and 16.82 to 18.47 Mg ha⁻¹ in 2000, but were not different between storage durations. Seed germination remained greater than 80% regardless of storage duration. Electrolyte leakage, based on conductivity, was 38–50% less with freshly harvested seed than seed stored for 4 years at 10 °C. However, neither the conductivity nor accelerated aging test were reliable predictors of field emergence. Kenaf seed stored up to 4 years at 10 °C retained germination rates acceptable for commercial use. Neither field emergence nor biomass yield was affected by seed storage duration.