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精氨酸、谷氨酰胺、谷氨酸、脯氨酸、天冬氨酸、天冬酰胺、乌氨酸和瓜氨酸可在大多数哺乳动物(包括猪)体内通过复杂的器官间代谢进行相互转化.小肠、肾脏和肝脏是这些氨基酸代谢的主要场所,皮质醇则是这些代谢的一个重要调控激素.由于这些氨基酸(除乌氨酸和瓜氨酸之外)通常大量地存在于植物和动物的组织蛋白中,所以养猪生产者习惯上不大关心猪日粮中精氨酸族氨基酸的添加问题.然而,最近的研究结果表明,这些氨基酸在养分代谢和免疫应答上起着重要的调控作用,从而会影响猪的饲料利用率.精氨酸和谷氨酸就是其中的典型代表,它们具有明确的功能,因此被广泛地应用于猪肉生产中.由瓜氨酸在小肠中合成减少所引起的精氨酸缺乏,是限制哺乳仔猪获得最佳生长性能的主要因素.酶学和代谢学研究结果均表明,对于7~21日龄哺乳仔猪而言,肠上皮细胞线粒体内N-乙酰谷氨酸的生物利用率低下应为通过谷氨酸和脯氨酸合成瓜氨酸受到阻碍负责.因此,无论在日粮中添加精氨酸还是口服N-胺基甲基麸胺酸(N-carbamylglutamate,N-乙酰谷氨酸的一种稳定的代谢类似物)都能提高哺乳仔猪的肌肉蛋白合成率和增重.此外,在早期断奶的仔猪日粮中添加谷氨酸已可防治其小肠萎缩.并且提高了仔猪的生长性能.值得注意的是,在妊娠30~114d的小母猪日粮中添加精氨酸,也会显著地提高产活仔数和窝初生重.大规模地使用饲料级精氨酸和谷氨酰胺为提高全球养猪生产的动物健康和养分利用率展现了广阔的前景. 相似文献
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试验旨在探讨仔猪在断奶后2周内日粮中添加精氨酸内源合成激活剂:N-氨甲酰谷氨酸(NCG)对精氨酸内源合成、肠道形态以及生长性能的影响。试验选取23±3日龄健康断奶阉公猪(杜×长×大)180头,并按体重、窝别等随机分为3个处理,处理1:对照组,饲喂基础日粮;处理2:精氨酸组,基础日粮添加1%L-精氨酸,处理3:NCG组,基础日粮添加0.05%NCG;每个处理6个重复(圈),每圈10头。试验末每圈选取1头仔猪采血并屠宰取空肠中段,测定血浆精氨酸浓度、血浆生长激素浓度以及空肠形态。经过试验,得出以下结论,断奶仔猪日粮中添加N-氨甲酰谷氨酸(NCG)可以改善断奶仔猪的生长,并不是通过提高采食量来实现的,而是通过增加内源精氨酸合成,提高血浆生长激素水平,以及恢复小肠形态等方面作用,最终促进断奶仔猪生长。 相似文献
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精氨酸、谷氨酰胺、谷氨酸、脯氨酸、天冬氨酸、天冬酰胺、鸟氨酸和瓜氨酸可在大多数哺乳动物(包括猪)体内通过复杂的器官间代谢进行相互转化。小肠、肾脏和肝脏是这些氨基酸代谢的主要场所,皮质醇则是这些代谢的一个重要调控激素。由于这些氨基酸(除鸟氨酸和瓜氨酸之外)通常大量地存在于植物和动物的组织蛋白中,所以养猪生产者习惯上不大关心猪日粮中精氨酸族氨基酸的添加问题。然而,最近的研究结果表明,这些氨基酸在养分代谢和免疫应答上起着重要的调控作用,从而会影响猪的饲料利用率。精氨酸和谷氨酸就是其中的典型代表,它们具有明确的功能,因此被广泛地应用于猪肉生产中。由瓜氨酸在小肠中合成减少所引起的精氨酸缺乏,是限制哺乳仔猪获得最佳生长性能的主要因素。酶学和代谢学研究结果均表明,对于7~21日龄哺乳仔猪而言,肠上皮细胞线粒体内N-乙酰谷氨酸的生物利用率低下应为通过谷氨酸和脯氨酸合成瓜氨酸受到阻碍负责:因此,无论在日粮中添加精氨酸还是口服N-胺基甲基麸胺酸(N—carbamylglutamate,N-乙酰谷氨酸的一种稳定的代谢类似物)都能提高哺乳仔猪的肌肉蛋白合成率和增重。此外,在早期断奶的仔猪日粮中添加谷氨酸已可防治其小肠萎缩,并且提高了仔猪的生长性能。值得注意的是,在妊娠30~114d的小母猪日粮中添加精氨酸,也会显著地提高产活仔数和窝初生重。大规模地使用饲料级精氨酸和谷氨酰胺为提高全球养猪生产的动物健康和养分利用率展现了广阔的前景。 相似文献
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Berkeveld M Langendijk P Verheijden JH Taverne MA van Nes A van Haard P Koets AP 《Journal of animal science》2008,86(12):3440-3449
The objective of the current study was to investigate whether plasma citrulline or intestinal fatty acid-binding protein (I-FABP) concentrations might be used as longitudinal markers for small intestinal function in piglets after weaning. Plasma citrulline and I-FABP concentrations were measured longitudinally in weaned and unweaned piglets, and related to intestinal absorption values (i.e., plasma mannitol and 3-xylose concentrations in a sugar absorption test). Within each litter (n = 10), 2 piglets with a close-to-litter-average BW were selected. At 20.8 +/- 0.4 d of age, the selected piglets per litter were either weaned conventionally (CW) or remained with the sow (UNW). One day before, and 0.5, 2, 4, and 7 d after weaning of the CW piglets, the selected piglets of both groups were subjected to a sugar absorption test. After a 2-h fast, piglets were administered an oral dose of 2 mL/kg of sugar solution, containing 50 mg/kg of mannitol and 100 mg/kg of 3-xylose. One hour after administration, a blood sample was collected from a jugular vein for determination of plasma I-FABP, citrulline, mannitol, and 3-xylose concentrations. Plasma I-FABP concentration showed great variation within treatments, and no difference was observed in plasma I-FABP concentrations between the CW and UNW treatments (P = 0.63). The absorption of 3-xylose was not different between treatments (P = 0.83). Mannitol absorption, however, was less in the weaned CW piglets compared with the UNW piglets (P = 0.003), with the nadir on d 4 postweaning. Weaning also reduced plasma citrulline concentrations in the CW treatment compared with the UNW treatment (P < 0.001). On d 4 and 7 postweaning, plasma citrulline concentrations of CW piglets were less (P < 0.001 and P = 0.0013) than preweaning values. Furthermore, in the CW treatment, plasma citrulline concentrations correlated with plasma mannitol concentrations at d 4 postweaning (r = 0.89, P = 0.008) and overall (r = 0.76, P = 0.001). Based on these results, plasma citrulline concentration seems to be a possible marker for monitoring intestinal function in pigs after weaning. 相似文献
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Postnatal changes of plasma amino acids in suckling pigs 总被引:9,自引:0,他引:9
Amino acids, ammonia, urea, orotate, and nitrate plus nitrite (stable oxidation products of nitric oxide) were determined in plasma of 1- to 21-d-old suckling pigs. Jugular venous blood samples were obtained from pigs at 1, 3, 7, 14, and 21 d of age for analysis of plasma amino acids and metabolites by HPLC and enzymatic methods. Plasma concentrations of arginine and its immediate precursors (citrulline and ornithine) decreased (P < 0.01) progressively (20 to 41%) with increasing age from 3 to 14 d. Plasma concentrations of glutamine declined (P < 0.01) progressively (10 to 31%) during the 1st wk of life. Plasma concentrations of branched-chain amino acids, threonine, and alanine decreased (P < 0.01) (5 to 12%) in 14- and 21-d-old pigs, compared with 1- and 3-d-old pigs. There were no postnatal changes (P > 0.05) in plasma concentrations of other amino acids. Plasma concentrations of ammonia increased (P < 0.01) by 18 and 46%, whereas those of nitrate plus nitrite decreased (P < 0.01) by 16 and 29%, in 7- and 14-d-old pigs, respectively, compared with 1- to 3-d-old pigs. Because arginine plays a crucial role in ammonia detoxification via the hepatic urea cycle and is the physiological substrate for nitric oxide synthesis, our results of the decreased plasma concentrations of arginine and nitrate plus nitrite, as well as the increased plasma ammonia concentration, indicate a hitherto unrecognized deficiency of arginine in 7- to 21-d-old suckling pigs. Arginine is an essential amino acid for piglets and has a great potential to enhance neonatal growth; therefore, further studies are necessary to elucidate the mechanism responsible for arginine deficiency in sow-reared piglets and to identify hormonal and metabolic means for improving neonatal arginine nutrition and growth. 相似文献
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由于精氨酸及其代谢产物具有广泛生物学作用,使得近年来研究者们在生理、生化和营养等方面对其作了大量的研究。母乳严重缺乏精氨酸,仔猪精氨酸内源合成对精氨酸的平衡起了重要作用,使得哺乳仔猪精氨酸的内源合成成为精氨酸营养的研究核心。通过对精氨酸内源合成路径和场所的了解,研究者们进一步研究了精氨酸内源合成的影响因素,主要有仔猪日龄、日粮中精氨酸的水平、乳酸盐和皮质醇的浓度等。本文就仔猪精氨酸内源合成的途径和场所、内源合成的量、影响因素以及精氨酸的需要量等进行了综述,重点阐述了精氨酸内源合成的影响因素。 相似文献
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精氨酸又称蛋白氨基酸,传统上,精氨酸不被认为是母仔猪的必需氨基酸,但是现代品种的猪生长更快,瘦肉率更高,孕育的胎儿更多,因此,对精氨酸有更高的生理需求量。大量研究表明,日粮中添加0.5%至1%精氨酸可以改善母仔猪的生产性能和繁殖性能。文章综合论述了母仔猪精氨酸的生理代谢和营养作用。 相似文献
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Jing Zhang Guochao Wu Anshan Shan Yu Han Yongcheng Jin Hengtong Fang 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2017,67(1-2):15-21
Forty-eight Min piglets at 7 days of age were randomly divided into two groups which were fed with 0% and 0.8% glutamine, respectively. Supplementation with 0.8% l-glutamine, the average daily gain (ADG) were significantly increased, and the concentrations of glutamine, glutamate, arginine and citrulline in plasma were significantly improved (P?.05). The expression of jejunal mucosa glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) were both significantly increased, and the concentration of malonaldehyde (MDA) was significantly reduced (P?.05). Furthermore, the villus height and the villus height to crypt depth ratio were significantly increased (P?.05). Glutamine supplementation significantly increased the expression levels of occludin mRNA and protein (P?.05), while the mRNA and protein expression levels of ZO-1 were not significantly affected. These results showed that dietary supplementation with glutamine contributed to improving intestinal development and whole-body growth in Min piglets. 相似文献
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功能性氯基酸——精氦酸(Arg)具有重要的生理、代谢和营养作用,几乎机体中所有组织均利用Arg合成胞浆蛋白和核蛋白,精氨酸在促进肌肉蛋白质合成、增强机体的免疫力、细胞分裂、伤口复尿和激素分泌等各种生理过程中,也都有着重要的角色。Arg为条件性必需氨基酸,在应激状态下和特殊生长阶段,为必需氨基酸,仔猪体内合成的精氨酸不能满足生理代谢需要。但是精氨酸的吸收与赖氨酸等桔抗,因此,对精氨酸及其内源性合成调控研究具有极大的应用价值和实践意义。精氨酸生素(Arginineactivatoradditive,AAA)是N-乙酰谷氨酸(N-acetylglutamate,NAG)的类似物,作为一种新型的功能性氨基酸,可以有效调控内源性精氨酸合成,进而促进动物生长性能。本文就精氨酸和精氨酸生素特别是其对机体内源性精氨酸合成调控及其在仔猪和母猪中的研究进展与应用作一综述。 相似文献
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Kristine L. Urschel Amanda R. Evans Paul B. Pencharz Ronald O. Ball 《Livestock Science》2007,108(1-3):41-44
Parenteral feeding can be used to induce intestinal atrophy in piglets, and this atrophy is believed to be associated with the inability of parenterally-fed piglets to maintain arginine status via synthesis. Glucagon-like peptide 2 (GLP-2) has been shown to maintain intestinal structure and blood flow during intravenous feeding. GLP-2 infusion was hypothesized to increase the rate of endogenous arginine synthesis from proline in parenterally-fed piglets receiving an arginine deficient diet. Male piglets (n = 10, 1.5–2.0 kg), fitted with jugular vein catheters for diet and isotope infusion, and femoral vein catheters for blood sampling (d 0), were allocated to a continuous infusion of either GLP-2 (10 nmol/kg/d) or saline into the jugular vein. Piglets received 2 d of a complete diet, followed by 5 d of an arginine deficient (0.60 g/kg/d) diet. A primed, constant infusion of [guanido-14C]arginine measured arginine flux (d 6), and of [U-14C]proline (d 7) measured proline conversion to arginine. There were no differences between groups in plasma ammonia, urea and arginine concentrations and arginine flux. Piglets receiving GLP-2 had a greater jejunal mucosal mass (P = 0.003) and a two-fold greater rate of arginine synthesis from proline (P = 0.03). This study indicates that the intestinal metabolism of circulating precursors may be important for arginine synthesis in parenterally-fed neonates. 相似文献
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Arginine catabolism in lactating porcine mammary tissue 总被引:3,自引:0,他引:3
In vivo studies have shown that the uptake of plasma arginine by the lactating porcine mammary gland greatly exceeds the output of arginine in milk, but little is known about the metabolic fate of arginine in this organ. The objective of this study was to quantify arginine catabolism via arginase and nitric oxide synthase pathways in the mammary tissue of sows on d 28 of lactation. Mammary tissue slices (approximately 60 mg) were incubated at 37 degrees C for 1 h in 2 mL of Krebs bicarbonate buffer containing 0.5 or 2 mM L-[U-14C]arginine, and arginine metabolites were measured using HPLC and radiochemical techniques. Rates of arginine utilization were similar to rates of urea production. Proline, ornithine, urea, glutamate, glutamine, CO2 and polyamines (putrescine + spermidine + spermine) were formed from arginine, accounting for 46, 31, 17, 2.3, 1.5, 0.22, and 0.30%, respectively, of the metabolized arginine carbons. Relatively small amounts of arginine were utilized for nitric oxide and citrulline synthesis, with citrulline accounting for 2% of the metabolized arginine carbons. Production of all arginine metabolites increased with increasing extracellular arginine concentrations from 0.5 to 2 mM, indicating a high capacity for arginine degradation. Consistent with the metabolic findings, the activities of arginases, ornithine aminotransferase, and pyrroline-5-carboxylate reductase were high, whereas those of pyrroline-5-carboxylate dehydrogenase, ornithine decarboxylase, and nitric oxide synthases were relatively low, and there was no proline oxidase, ornithine carbamoyltransferase or pyrroline-5-carboxylase synthase activity in the mammary tissue. Our results demonstrate for the first time that proline, ornithine, and urea were the major products of arginine catabolism via the arginase pathway in lactating porcine mammary tissue and provide a biochemical basis to explain a relative enrichment of proline but a relative deficiency of arginine in sow's milk. 相似文献
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Tércia Cesária Reis de Souza Araceli Aguilera Barreyro Gerardo Mariscal-Landín 《畜牧与生物技术杂志(英文版)》2014,(1):74-79
Background: Many studies have investigated endogenous loss of proteins and amino acids (AAs) at the ileal level in growing pigs. However, only a few studies have researched this subject in piglets. Knowledge regarding AA ileal digestibility in piglets would be helpful during the formulation of diets for weaning piglets, rather than just using coefficients obtained in growing pigs. Therefore, in this study, we sought to estimate endogenous protein and AA ileal losses in piglets. Furthermore, apparent and true ileal digestibility (AID and TID) of protein and AAs from casein were measured. Results: The average flow of protein was 20.8 g/kg of dry matter intake (DMI). Basal protein loss, as estimated by regression, was 16.9 g/kg DMI. Glutamic acid, arginine, and aspartic acid (2.2, 1.4, and 1.2 g/kg DMI, respectively) were the AAs for which greater losses were seen. The AID of protein and AAs increased as the protein level in the diet increased. A higher increment in AID was observed between diets with 80 and160 g CP/kg of feed; this finding was mainly attributable to increases in glycine and arginine (46.1% and 18%, respectively). The TID of protein was 97.8, and the TID of AAs varied from 93.9 for histidine to 100.2 for phenylalanine. Conclusions: The basal endogenous protein loss in piglets was 16.9 g/kg DMI. Endogenous protein was rich in glutamic acid, aspartic acid, and arginine, which represented 32.7% of endogenous protein loss in weaning piglets. The TID of casein was high and varied from 93.0 for histidine to 100.2 for phenylalanine. 相似文献