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维生素E缺乏对雏鸡脾脏淋巴细胞Caspase-3活性及Caspase-3mRNA表达的影响 总被引:1,自引:0,他引:1
用低维生素E(VE)和添加不饱和脂肪酸的日粮饲喂雏鸡建立VE缺乏雏鸡模型,采用比色法和半定量RT-PCR法分别检测脾脏淋巴细胞Caspase-3活性及Caspase-3 mRNA的表达水平。结果显示,VE缺乏组雏鸡脾脏淋巴细胞Caspase-3活性和Caspase-3 mRNA丰度高于对照组,组间差异均极显著(P<0.01)。结果表明,Caspase-3活性及Caspase-3 mRNA丰度的改变,是VE缺乏雏鸡脾脏淋巴细胞凋亡的调控机制之一。 相似文献
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本文旨在研究一氧化氮(NO)在硒缺乏致鸡脾脏淋巴细胞凋亡中的作用.选择1日龄健康海蓝褐雏鸡80只,随机分为对照组和缺硒组2个组,每组4个重复,每个重复10只鸡,对照组鸡饲料中硒含量为0.282 mg/kg,缺硒组鸡饲料中硒含量为0.032 mg/kg.分别于30、45、60和75日龄每组随机抽取10只鸡采样,测定脾脏和血清谷胱甘肽过氧化物酶(GSH-Px)活性、NO含量、一氧化氮合酶(NOS)活性以及脾脏和全血中硒含量的变化;检测脾脏中诱导型一氧化氮合酶(iNOS)mRNA表达量变化和淋巴细胞的凋亡情况.结果表明,与对照组比较,缺硒组鸡脾脏和全血中硒含量降低;脾脏和血清中GSH-Px活性降低;NO含量、NOS活性及iNOS mRNA表达量升高;脾脏淋巴细胞DNA出现降解,呈现梯形条带,电镜下细胞核皱缩,核染色质边聚、固缩,线粒体肿胀,嵴溶解甚至消失,内质网排列紊乱、肿胀等呈现典型的细胞凋亡现象.本试验结果提示硒缺乏可引起鸡脾脏及血清NO代谢发生改变,导致脾脏淋巴细胞发生凋亡,表明NO在硒缺乏致鸡脾脏淋巴细胞凋亡中具有重要作用. 相似文献
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试验旨在研究饲粮中维生素E缺乏对雏鸡生长性能、脾脏组织和脾脏淋巴细胞形态结构、脾脏淋巴细胞凋亡相关蛋白和基因表达水平的影响。选取1日龄雄性雏鸡160羽,随机分为2组,饲粮维生素E缺乏试验组的预混料中不添加维生素E,对照组额外添加维生素E(30 mg/kg),每组各80羽,每组10个重复,每个重复8羽,试验期40 d。结果表明:试验组雏鸡平均日增重低于对照组(P<0.05),耗料增重比高于对照组(P<0.05);试验组雏鸡脾脏组织和脾脏淋巴细胞中维生素E含量低于对照组(P<0.05);试验组雏鸡脾脏组织和脾脏淋巴细胞结构出现损伤;试验组雏鸡脾脏淋巴细胞中Caspase-3活性高于对照组(P<0.05);试验组雏鸡脾脏淋巴细胞中Bcl-2的mRNA表达量低于对照组(P<0.05),p53和Caspase-3的mRNA相对表达量高于对照组(P<0.05)。由此可见,饲粮中维生素E缺乏将导致雏鸡生长性能显著降低;维生素E缺乏通过提高脾脏淋巴细胞中Caspase-3活性,影响Bcl-2、p53和Caspase-3基因表达诱导脾脏淋巴细胞凋亡。 相似文献
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细胞凋亡是生物有机体在进化过程中形成的一种主动性细胞死亡方式,它受细胞外微环境和细胞内遗传物质的调控.透射电镜下观察发现,维生素E缺乏雏鸡免疫细胞具有典型的凋亡特征[1],但其凋亡机制尚不清楚.p53是一种促凋亡基因,bcl-2是一种抗凋亡基因,二者在细胞凋亡过程中具有重要的调控作用.维生素E缺乏对雏鸡脾脏淋巴细胞凋亡基因p53和bcl-2mRNA表达的影响未见有文献报道.本研究通过建立维生素E缺乏雏鸡模型,采用半定量RT-PCR法检测脾脏淋巴细胞内p53和bcl-2mRNA表达水平,旨在揭示维生素E缺乏雏鸡淋巴细胞凋亡的基因调控机制. 相似文献
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VE缺乏对雏鸡脑组织的氧化状态及神经细胞凋亡的影响 总被引:3,自引:0,他引:3
采用低VE和添加不饱和脂肪酸的日粮饲喂1日龄雏鸡,建立了VE缺乏实验动物模型。检测其脑组织(大脑和小脑)中丙二醛和活性氧的含量及神经细胞凋亡的数量,并进行了相关性分析。结果表明,VE缺乏雏鸡脑组织中的丙二醛和活性氧含量及神经细胞凋亡的数量显著高于健康对照组(P<0.05),细胞凋亡的数量与大、小脑组织中丙二醛和活性氧含量呈显著的相关性(相关系数分别为0.7502、0.8155和0.8170、0.6931),说明VE缺乏雏鸡脑组织处于氧化应激状态,并可诱导脑神经细胞凋亡。 相似文献
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枸杞多糖对雏鸡淋巴细胞体外增殖及分泌IL-2的影响 总被引:1,自引:0,他引:1
为探讨枸杞多糖(LBP)对雏鸡T、B淋巴细胞体外增殖的影响,将不同浓度的LBP分别加入到用植物血凝素(PHA)刺激的体外培养的雏鸡胸腺、外周血、脾脏淋巴细胞中,以及用细菌脂多糖(LPS)刺激的体外培养的雏鸡法氏囊、外周血、脾脏淋巴细胞中,分别用MTT比色法和ELISA法测定淋巴细胞增殖的变化以及IL-2的分泌量.结果显示:(1)LBP在一定浓度范围内时,均能刺激T、B淋巴细胞的增殖和促进IL-2的分泌,且随着多糖浓度的增加而增加;(2)LBP对同一器官来源的T、B淋巴细胞的OD值随着雏鸡日龄的增长均呈现增长趋势;(3)同浓度LBP对相同日龄雏鸡胸腺T淋巴细胞和法氏囊B淋巴细胞的OD值均高于外周血和脾脏的T、B淋巴细胞OD值.结果表明LBP能够促进雏鸡淋巴细胞的增殖和IL-2的分泌,并且对淋巴细胞增殖的促进作用有一定的量效、时效关系和组织相关性. 相似文献
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魏静 《四川畜牧兽医学院学报》2009,(4):28-32
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。 相似文献
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本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。 相似文献
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REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air. 相似文献
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以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。 相似文献
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Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo. 相似文献