共查询到20条相似文献,搜索用时 31 毫秒
1.
Helopeltis theivora, one of the major pests of tea from North east India, reported to acquire tolerance against commonly used synthetic pesticides. Study on relative toxicity of both sexes of H. theivora along with their detoxifying enzyme levels were carried out to assess developemnt of sex-based tolerance of pest against the pesticides. The present study showed that female develop more tolerance among H. theivora population than male. Relative toxicity tests showed that LC50 was higher in female than male H. theivora. This result reflects in general, sex level difference in tolerance status of the pest along with corresponding changes in the quantities of detoxifying enzymes. Female of H. theivora showed significantly higher general estearse and glutathione-S-transferase activities than that of male; however there was no significant difference in cytochrome P450 activity. The total body lipid content was also found to be higher in female than male, which indicated female attribute to be more tolerant than male. Body weight of female was significantly higher than that of male, which showed positive correlation with general esterase and glutathione-S-transferase. Hence, on application of recommended dose of pesticide, chance of survivability of female is better in field, leading to addition of more tolerant forms. 相似文献
2.
Ramzi Mansour Pompeo Suma Gaetana Mazzeo Kaouthar Grissa Lebdi Agatino Russo 《Phytoparasitica》2011,39(4):369-376
Control of the invasive vine mealybug Planococcus ficus (Signoret) can be achieved through integration of different management strategies such as the application of chemical treatments and biological control. However, compatibility between these control methods is considered crucial when adopting integrated pest management (IPM) programs against this insect pest. The recently used insecticides Prev-Am®, a contact biopesticide, and spirotetramat, a systemic tetramic acid insecticide, were assessed in laboratory conditions for their side effects on the vine mealybug parasitoid Anagyrus sp. near pseudococci with reference to chlorpyriphos-methyl, an organophosphate insecticide conventionally used for mealybug control in vineyards. Regarding contact toxicity, chlorpyriphos-methyl caused 100% parasitoid mortality 24 h after treatment, but parasitoid mortality from Prev-Am® or spirotetramat was almost absent. Neither the total parasitoid offspring/female nor the sex ratio of the progeny was negatively affected by Prev-Am® or spirotetramat relative to the untreated control. According to the IOBC classification for laboratory trials, both Prev-Am® and spirotetramat were rated harmless (IOBC category 1), whereas chlorpyriphos-methyl was categorized as harmful (IOBC category 4) to A. sp. near pseudococci. None of the insecticides adversely affected the development of the parasitoid pupal stage inside mealybug mummies or the survival of the emerged parasitoids. The practical implications of the results for implementing mealybug IPM programs in vineyards are emphasized and discussed. 相似文献
3.
The present study was conducted to determine egg and larval parasitoids of the beet armywormSpodoptera exigua Hübner (Lepidoptera: Noctuidae), which is an important but sporadic pest in Turkey. High beet armyworm population levels were recently observed in fields of first and second crop maize in the southeast Mediterranean region of Turkey. The parasitoid species complex and its impact on the pest were analyzed in a 4-year study in first and second crop maize. The braconid larval parasitoidsMicroplitis rufiventris Kokujev,M. tuberculifer Wesmael,Meteorus ictericus Nees,Chelonus obscuratus (Herrich Schäffer) (an egg-larval parasitoid),Apanteles ruficrus (Haliday); the ichneumonid larval parasitoidsHyposoter didymator (Thunberg) andSinophorus xanthostomus Gravenhorst; and the egg parasitoidTrichogramma evanescens (Westwood) were found to be the natural enemies attacking the pest. Among the parasitoid species the solitary endoparasitoidH. didymator was the most prevalent species, being reared from 40.5% of the parasitized larvae found. Higher parasitism rates were recorded on first crop than on second crop maize in every year. Possible reasons for this difference in larval parasitism between two growing seasons include lower population of the pest and reduced insecticide applications in first crop maize fields which permitted higher parasitism. However, parasitoid activity was insufficient to counterbalance the population growth of the pest on subsequent second crop maize. 相似文献
4.
This study provide information on the first occurrence in Italy of the Coleoptera Cerambycidae Chlorophorus annularis (Fabricius, 1787). It is a species naturally and widely distributed in Asia and in oriental tropical and subtropical areas. This cerambyx is a post-harvest pest of bamboo belonging to several genera as Bambusa, Dendrocalamus, Phyllostachys, Sinocalamus, but it can also infest cultivated plants as Citrus spp., Gossypium, Liquidambar spp., Saccharum officinarum L., Vitis spp., and Zea mays L. 相似文献
5.
Cherif Asma Ichaoui Ons Barhoumi-Attia Sabrine Lebdi-Grissa Kaouthar 《Phytoparasitica》2018,46(1):105-113
Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is classified as one of the most harmful pest of tomato crops. Many species of predators and parasitoids including Trichogramma cacoeciae (Marchal) (Hymenoptera: Trichogrammatidae) are noted as potential candidates used for biological control of this pest. Therefore, the use of selective insecticides is critical to conserve and protect natural enemies in the field. This study assessed the side effects of insecticides on different development stages of T. cacoeciae under laboratory conditions. For this, eleven pesticides such as: Indoxacarb, spiromesifen, cyromazin, chlorfenapyr, cypermethrin, diafenthiuron, chlorantraniliprole, spinosad, azadirachtin, Bacillus thuringiensis (Bt) and virus HaNPV were tested. This study shows that indoxacarb, spiromesifen, chlorfenapyr, cypermethrin, diafenthiuron and spinosad had a negative effect on immature stages of Trichogramma. All insecticides residues on tomato leaves were found to be toxic to Trichogramma adults except azadirachtin, Bt and virus HaNPV. Therefore, the use of the tested natural products (azadirachtin, Bt and HaNPV) at the recommended doses is viable, having no negative impact on T. cacoeciae in tomato crops. 相似文献
6.
The ficus whitefly Singhiella simplex is an urban area pest which originated from Asia.Singhiella simplex (Singh) (Hemiptera: Aleyrodidae) is for the first time recorded for the whitefly fauna in Turkey. The pest was found on Ficus microcarpa in Antalya province, Turkey. Its identification, host plants, and distribution across the world are briefly described. 相似文献
7.
The maize armyworm, Mythimna separata is a polyphagous insect pest of sporadic occurrence. Thiamethoxam is a neonicotinoid insecticide used for the management of many pests in many parts of the world. To develop a resistance management strategy, selection for resistance, the larval fitness parameters and the biochemical mechanisms of resistance to thiamethoxam were studied for thiamethoxam-selected and susceptible M. separata strains based on laboratory observations. The results of our bioassay showed that the thiamethoxam-selected strain was 17.03-fold more resistant than the susceptible strain. The thiamethoxam-selected strain had prolonged larval durations, lower pre-pupal weight of males, and a longer development time from egg to adult than the susceptible strain. The biochemical analyses showed that the GST, CarE and cytochrome P450 enzymes are associated with the development of thiamethoxam resistance in the thiamethoxam-selected strain of M. separata. In this study, the occurrence of resistance may cost developmental fitness for the thiamethoxam-selected strain and provide useful information for designing management strategies to delay resistance. 相似文献
8.
V. M. Malathi Sushil Kumar Jalali Vazhakkala Jose Lyju R. Gandhi Gracy Ravi Prabhakar More Rangasamy Anandham Apoothy Thulasi Thiruvengadam Venkatesan 《Phytoparasitica》2017,45(5):683-693
Nilaparvata lugens (Stål) is one of the major pests of rice throughout tropical and temperate Asia. Indiscriminate use of insecticides for suppressing N. lugens has resulted in the development of resistance to multiple insecticide classes, causing frequent control failures in the field. Analysis of gut bacterial diversity within an insect host is the initial step towards understanding the ecological roles of the symbionts. Present study aimed to survey the bacterial diversity associated with laboratory-reared (insecticide-susceptible) and field-collected (insecticide-resistant) populations of N. lugens by culture-dependent and PCR-Denaturing Gradient Gel Electrophoresis (DGGE) methods. Seventeen bacterial isolates were obtained by the culture-dependent method. Molecular characterization using the 16S rRNA gene and phylogenetic analysis revealed that the isolates belonged to Firmicutes and Proteobacteria. Taxonomic assignment placed these isolates into seven families representing 10 genera. Enterobacteriaceae was the most dominant family with its occurrence in four out of the five populations studied. The DGGE profiles indicated a low complex gut bacteria associated with N. lugens with limited number of bands. The Shannon-Wiener index ranged from 0.898 in insecticide-susceptible population to 0.946–1.035 in resistant populations. Sequencing and phylogenetic analysis revealed that the DGGE bands belonged to Firmicutes, Proteobacteria and Bacteriodetes. Results of this study illustrated that gut bacterial community associated with N. lugens is dominated by Proteobacteria and Firmicutes. Present findings could provide the basis for future work on the possible role of the bacterial symbionts in insecticide resistance and to formulate potential resistance management strategies. 相似文献
9.
Aline Koch Elke Stein Karl-Heinz Kogel 《European journal of plant pathology / European Foundation for Plant Pathology》2018,152(4):1003-1010
RNA-based disease control has shown great potential for controlling pest and diseases in crop plants. While delivery of inhibitory noncoding double-stranded (ds)RNA by transgenic expression is a promising concept, it requires the generation of transgenic crop plants, which may cause substantial delay for application strategies depending on the transformability and genetic stability of the crop plant species. Focusing on agronomic important barley - Fusarium spec. pathosystems, we have sought for alternative strategies to apply dsRNAs for fungal control. Recently, we have demonstrated that a spray application of a long noncoding dsRNA termed CYP3RNA, which targets the three fungal Cytochrome P450 lanosterol C-14α-demethylase genes FgCYP51A, FgCYP51B, and FgCYP51C, inhibits Fusarium graminearum (Fg) on barley leaves (Koch et al., PLoS Pathogens, 12, e1005901, 2016). Here we show that another Fusarium species, F. culmorum (Fc), also is sensitive to CYP51-derived dsRNAs. Treating Fc with various dsRNAs targeting the genes FcCYP51A, FcCYP51B and FcCYP51C was destructive to the fungus and resulted in growth retardation in in vitro cultures. We discuss important consequences of this finding on future RNA-based disease control strategies. Given the ease of design, high specificity, and applicability to diverse pathogens, the use of target-specific dsRNA as an anti-fungal agent offers unprecedented potential for novel plant protection strategies. 相似文献
10.
Malkie Spodek Yair Ben-Dov Lily Mondaca Alex Protasov Eyal Erel Zvi Mendel 《Phytoparasitica》2018,46(1):45-55
In Israel, the cotton mealybug Phenacoccus solenopsis Tinsley, an invasive scale insect, was reported for the first time in the Jordan Valley in 2008 on basil (Ocimum basilicum) and bell pepper (Capsicum annuum). This mealybug is highly polyphagous with economic and environmental impacts. Since then, Ph. solenopsis has spread to almost every region of Israel and developed high populations on several ornamental plants, mainly Hibiscus sp. (Malvaceae) and Lantana sp. (Verbenaceae). It has become a pest in greenhouses, mainly on bell pepper, tomato, and eggplant (Solanaceae) and a serious threat in cotton fields. Fourteen species of insect natural enemies have been found in association with Ph. solenopsis in Israel the common ones were: Aenasius arizonensis (Girault) (Hym. Encyrtidae), Cheilomenes propinqua (Mulsant), Hyperaspis vinciguerrae (Capra); H. polita Weise, Exochomus nigripennis (Erichson), Parascymnus varius Kirsch and Scymnus flagellisiphonatus (Fursch) (Col., Coccinellidae). To date, the population density of Ph. solenopsis in Israel is steeply decreasing in most regions of Israel due to the activity of A. arizonensis. An identification key to distinguish between adult females of the eight species belonging to the genus Phenacoccus in Israel is also provided. 相似文献
11.
12.
13.
Evin Polat Akköprü 《Phytoparasitica》2018,46(4):511-520
The development, reproduction, and population growth parameters of cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae), were studied on three different Cucumis sativus cultivars (Beith Alpha Bal?kesir, B.T. Bursaalpha, Beith Alpha MR,) under controlled conditions (25?±?1 °C, 65?±?5% RH, and a photoperiod of 16:8 (L:D) h. Life table parameters were estimated according to age-stage, two-sex life table method. Results indicated that the Bt Bal?kesir are favorable hosts for A. gossypii because of the shorter preadult developmental time, lower preadult mortality rate, and higher total fecundity on this cultivar. The intrinsic rate of increase (r?=?0.4214 d-1), the net reproduction rate (R0?=?51.80 offspring), and the finite rate of increase (λ?=?1.5242 d-1) values were higher on the Bt Bal?kesir. The effect of cucumber cultivars on the biology of A. gossypii was determined as significant and it was found that cv. Bt Bal?kesir was the most convenient cucumber cultivar in terms of the life cycle of A. gossypii. For an ecological and economic pest control, against aphids use of resistant or less favourable crop cultivars is considered one of the important components of integrated pest management. Therefore, results acquired might be used in pest management program that will be prepared for this aphid. 相似文献
14.
Yield and quality loss of carrot (Daucus carota L. cv. Nerac) caused by Pratylenchus penetrans and the population dynamics of this nematode were studied in a climate controlled glasshouse. A range of 12 nematode densities was used at three different seed densities of carrot; 2, 4 and 18 seeds pot?1. Seinhorst’s yield loss model; y?=?m?+?(1 - m) 0.95 Pi/T-1 for Pi?>?T; y?=?1 for Pi?≤?T for Tylenchina was fitted to the yield and quality loss data. Seinhorst’s model for population dynamics of migratory nematodes with multiple generations; \( Pf=M* Pi/\left( Pi+M/a\right) \) was fitted to the data of the final population densities (Pf). P. penetrans had a significant impact on carrot taproot yield and its quality. The tolerance limits for the relative carrot taproot yield (T y) were 1.51, 1.88, and 1.37 and those of quality yields (T q) were 0.67, 0.18, and 0.40 P. penetrans (g dry soil)?1 at 2, 4 and 18 seeds pot?1, respectively. Both the minimum yield (0.20, 0.29, and 0.60) and the minimum quality yield (0.05, 0.07, and 0.20), expressed as a proportion, increased with seed density at 2, 4 and 18 seeds pot?1, respectively. The model for population dynamics fitted well to the Pf data obtained. The maximum multiplication rates (a) were 19.58, 9.99, and 17.54, while the maximum population densities (M) were 49.86, 43.21, and 60.37 P. penetrans (g dry soil)?1 at 2, 4, and 18 seeds pot?1, respectively. Carrot cv. Nerac can be considered a good host for P. penetrans. 相似文献
15.
Lysiphlebus fabarum Marshall is the main parasitoid of the black bean aphid, Aphis fabae Scopoli. Lethal and sublethal effects of insecticides, thiacloprid+deltamethrin, pirimicarb and pymetrozine were evaluated on the parasitoid under laboratory conditions. One-day-old mummies were exposed to the recommended field concentration of either insecticides via dipping method. Adult emergences were reduced by 82.67, 19.98 and 10.67 % for thiacloprid+deltamethrin, pirimicarb and pymetrozine treatments, respectively. Thiacloprid+deltamethrin had the most adverse effect on the fecundity of the emerged females, while pirimicarb and pymetrozine did not have such effects. According to International organization for biological control (IOBC) insecticide toxicity classification, thiacloprid+deltamethrin resulted to be moderately harmful (E = 97.39%), whereas pirimicarb (E = 15.78%) and pymetrozine (E = 5.15%) were harmless. Thiacloprid+deltamethrin negatively affected five of the estimated demographic parameters (GRR, R 0 , r m , λ and T ). Pirimicarb negatively affected GRR, R 0 and T, while it had no adverse effects on r m and λ. None of the studied demographic parameters were affected by pymetrozine. Our results suggest that pirimicarb and pymetrozine can be considered as safe for L. fabarum, but that thiacloprid+deltamethrin can have serious detrimental of this parasitoid in the field. 相似文献
16.
Olivera Petrović-Obradović Andja Radonjić Ivana Jovičić Andjeljko Petrović Korana Kocić Željko Tomanović 《Phytoparasitica》2018,46(5):653-660
New alien species of aphids (Illinoia liriodendri (Monell), Wahlgreniella nervata (Gillete), Takecallis arundicolens (Clarke) and Tinocallis takachihoensis Higuchi) have been found for the first time in Serbia. These records are new for the Balkan Peninsula. Data of Ericaphis scammelli (Mason), species recently mentioned in Serbia, are also presented in the text. The species have been found during the last five years at four sites (Belgrade, Zemun, Mladenovac and Bojnik) on five cultivated plants. Ericaphis scammelli is a pest of blueberry and a vector of Blueberry scorch virus. The other four develop colonies on decorative plants: I. liriodendri has been found on Liriodendron tulipifera, W. nervata on Rosa sp., T. arundicolens on Pseudosasa japonica and T. takachihoensis on Ulmus x hollandica. The origin of three of the species (E. scammelli, I. liriodendri and W. nervata) is North America and two (T. arundicolens and T. takachihoensis) have come from East Asia. Their morphology, biology and the damage they inflict are discussed here, as well as their potential economic and environmental threat. Only parthenogenetic viviparous females of all 5 species have been collected. Their biology has not been studied enough in Europe and needs further examination. Original drawings of all species are presented. 相似文献
17.
Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most serious pests of tomato, capable of causing 100% crop losses under favourable conditions. We studied the effect of different host plants on developmental and population parameters of this pest at 25?±?0.5 °C temperature, 70?±?5% relative humidity and 12 L: 12D photoperiod. Host plant had significant effect on the developmental biology and the population growth parameters of the pest. The leafminer developed fastest on tomato leaves and slowest on potato tubers. Population growth parameters like intrinsic rate of increase, net reproductive rate, finite rate of increase, doubling time and weekly multiplication rate of T. absoluta were highest on tomato leaves and lowest on potato tubers. Mean generation time was minimum on tomato leaves and maximum on potato tubers. Females developed on tomato leaves were more fecund than other hosts. Though, tomato was found to be the most suitable host plant of T. absoluta, yet, the pest developed and grew successfully on other alternate hosts like potato (Solanum tuberosum L.), brinjal (Solanum melongena L.) and pepino (Solanum muricatum Aiton). These hosts can, therefore, play an important role in the survival, population build up and overwintering of the miner. Further, under favourable conditions the miner can become a serious pest on these crops and need to be monitored on these crops as well. 相似文献
18.
The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is a major pest of pepper. In this study, we collected data on the development, survival, fecundity, and proportion of apterous and alate forms of green peach aphid reared on five commercial pepper cultivars (Amiral, Erciyes, Mert, Mertcan, and Naz) at 25?±?1°C, 60?±?5% RH, and a photoperiod of 16:8 (L:D) h. We analyzed the life history raw data by using the age-stage, two-sex life table. The shortest development time (6.66 days) and highest fecundity (62.68 individuals) occurred on the Erciyes cultivar. The highest intrinsic rate of increase (r?=?0.332 d?1), finite rate of increase (λ?=?1.394 d?1), net reproductive rate (R0?=?62.7 offspring) and shortest mean generation time (T?=?12.45 d) also occurred on the Erciyes cultivar; while the lower fitness occurred on the Amiral, Mertcan and Naz cultivars. Our results compared differences in the population growth rate of M. persicae on different pepper cultivars. This information will be useful to individuals working in pest management programs particularly those involving M. persicae. 相似文献
19.
The melon fruit fly, Bactrocera cucurbitae (Coquillett) poses a serious threat to cucurbit production worldwide. Its management by conventional means remains difficult due to their proclivity to oviposite in fruit. In view of the increasing environmental safety concerns, use of biocontrol agents for sustainable pest control holds immense potential. Given this, the study intended to identify the cultivable bacteria inhabiting the intestinal tract of adult male and female melon fruit flies (Bactrocera cucurbitae) separately from field-collected population, and to assess the attractiveness of these bacteria to the fly. All selected bacterial isolates were identified and characterized based on morphological, biochemical and 16S rRNA gene sequence. Bacterial community identified in the gut of B. cucurbitae predominantly composed of Enterobacteriaceae followed by Staphylococcaceae, Enterococcaceae, Bacillaceae and Brucellaceae. Further, the laboratory bioassay was employed to examine the attractiveness of the supernatant as well as whole culture broth of 10 different species of bacteria to B. cucurbitae adults. Among these, Klebsiella oxytoca and Citrobacter freundii followed by Bacillus cereus were found highly attractive to fruit flies. The field experiment using supernatant of two bacteria demonstrated that the K. oxytoca was significantly more attractive to female flies followed by C. freundii. 相似文献
20.
C. M. Wistrom G. K. Blaisdell L. R. Wunderlich M. Botton Rodrigo P. P. Almeida K. M. Daane 《European journal of plant pathology / European Foundation for Plant Pathology》2017,147(4):937-941
Grapevine leafroll disease is associated with several species of phloem-limited grapevine leafroll-associated viruses (GLRaV), some of which are transmitted by mealybugs and scale insects. The grape phylloxera, Daktulosphaira vitifoliae (Fitch) Biotype A (Hemiptera: Phylloxeridae), is a common vineyard pest that feeds on the phloem of vine roots. There is concern that these insects may transmit one or more GLRaV species, particularly GLRaV-2, a species in the genus Closterovirus. A field survey was performed in vineyards with a high incidence of grapevine leafroll disease and D. vitifoliae was assessed for acquisition of GLRaV. In greenhouse experiments, the ability of D. vitifoliae to transmit GLRaV from infected root sections or vines to co-planted virus-free recipient vines was tested. There were no GLRaV-positive D. vitifoliae in the field survey, nor did D. vitifoliae transmit GLRaV-1, ?2, ?3, or -4LV in greenhouse transmission experiments. Some insects tested positive for GLRaV after feeding on infected source vines in the greenhouse, however there was no evidence of virus transmission to healthy plants. These findings, in combination with the sedentary behaviour of the soil biotype of D. vitifoliae, make it unlikely that D. vitifoliae is a vector of any GLRaV. 相似文献