Changes in contribution of white clover to canopy structure in perennial ryegrass/white clover swards in response to N fertilizer

Twelve plots were laid down on an existing perennial ryegrass/white clover sward, one plot in each of six replicated blocks receiving 100 kg  N ha⁻¹ (100N) and one plot receiving no N (0N). Biomass, canopy development (stratified cuts and point quadrat records at 2–4-week intervals) and changes in stolon population density were recorded during one 8-week regrowth period (25 July–23 September) to investigate the likely causes of N effects on white clover in mixed swards.
Over the period, N fertilizer resulted in an increase of 74% in perennial ryegrass biomass and a reduction of 24% in white clover biomass. There was also a reduction of 44% in stolon growing point density, mainly due to lower density of younger stolon branches. White clover's contribution to the upper three leaf area index (LAI) units (taken as an estimate of the proportion of photosynthetically active radiation (PAR) intercepted) was, on average, 70% at 0N producing 74% of the sward biomass, compared with 46% contribution to interception and 37% contribution to biomass at 100N.
While there was no evidence of overtopping, it is concluded that N fertilizer application increased the LAI of perennial ryegrass in the upper layers of the canopy thereby reducing the share of available PAR to white clover. This, coupled with a lower radiation use efficiency at high N and lower population density, results in white clover's reduced performance in mixed swards receiving N fertilizer.     

Effects of spring fertilizer nitrogen and sward height on production from perennial ryegrass/white clover swards grazed by beef cattle

An experiment was carried out to compare the effects of two compressed sward height treatments, each at two fertilizer nitrogen (N) treatments (0 and 50 kg ha⁻¹ in spring), on the date of turnout and liveweight gain of steers grazing a perennial ryegrass/white clover ( Lolium perenne/Trifolium repens ) sward sited on clay loam in south-west England. The sward height treatments were 6 cm all season, and 4 cm in the spring rising to 6 cm in June; these were maintained using continuously variable stocking with Hereford × Friesian steers. Cattle were turned out on average 11·5 days earlier on the 4-cm sward height compared with the 6-cm sward height treatment. Liveweight gain early in the season was lower on the 4-cm swards than on the 6-cm swards. Liveweight gain ha⁻¹ over the whole season was similar for the two sward height treatments. Fertilizer N did not affect turnout date or liveweight gain.     

The effect of fertilizer nitrogen rate, white clover variety and closeness of cutting on herbage productivity from perennial ryegrass/white clover swards

Four varieties of white clover (small-leaved cv. Aberystwyth S184. medium-leaved cv. Grasslands Huia and large-leaved cvs Linda and Olwen) were sown at 3 kg ha^-1 together with 10 kg ha^-1 perennial ryegrass cv. Talbot. Herbage productivity was measured for three harvest years, 1979-81, over four annual rates of fertilizer N (0,120,240 and 360 kg ha^-1) and two closeness of cutting treatments (80 and 40 mm from ground level). A simulated grazing regime of six cuts per year at 3- to 6-week intervals was used.
Production of total herbage DM was increased by increasing N rate; mean annual DM production ranged from 783 1 ha^-1 with no N to 11701 ha^-1 at 360 kg ha^-1 N. Mean herbage response to N (kg DM per kg N applied) was 73,90 and 108 for the three successive N increments relative to no N. Mean white clover DM production was reduced from 4 14t ha^-1 with no N to 051 t ha^-1 at 360 kg ha^-1 N.
The large-leaved clover varieties were more productive than the small- or medium-leaved varieties at all N rates. Close cutting increased total herbage and white clover by a mean annual 16% and 31%. respectively. White clover varieties did not interact with either N rate or closeness of cutting.
It is concluded that repetitive N application over the growing season is incompatible with white clover persistence and production, even with large-leaved clover varieties or with close cutting, two factors which improved clover performance in the experimental swards.     

A comparison of diploid and tetraploid perennial ryegrass and tetraploid ryegrass/white clover swards under continuous sheep stocking at controlled sward heights. 1. Sward characteristics

Two 1·0 ha plots of a late-heading diploid perennial ryegrass (var. Contender) and a late-heading tetraploid ryegrass (var. Condesa), and two 1·4 ha plots of the tetraploid with Aberystwyth S184 small-leaved white clover, were direct sown in May 1987. Over the three years 1988–90 they were continuously stocked by Mule ewes with Suffolk-cross twin lambs, from early April to the end of August, at a target sward surface height (SSH) of 4–6 cm on one set of plots (constant swards) and, on the other set, al 4–6 cm rising after June to a target 6–8 cm (rising swards). The heights were achieved by variable stocking. Fertilizer N was applied only to the grass plots at the rate of 150- 180kgN ha^-1 annually.
SSH was mainly within the target 4–6 cm, after higher initial heights at turnout in 1988and 1990. Mean heights of the constant swards (April- August) averaged 5·53, 4·43 and 5·04cm in the three years. The rising swards (July-August) increased in height over the constant swards by an average of 0·88, 0·48 and 0·55 cm, in successive years.
Clover content of the herbage mass dry matter in the grass/clover swards increased over each grazing season to average 13·0, 26·5 and 21·2% in the three years, with a high mean stolon density of 130 in m^-2 in August 1990. Ryegrass tiller densities in year 3 were 23% higher in the diploid than in the tetraploid swards, which had 43% more than the 10000 tillers m^-2 of the tetraploid ryegrass/clover swards.
It is concluded that the combination of a densely stoloniferous small-leaved clover with the open growth habit of a tetraploid ryegrass can achieve swards of high clover content under continuous sheep stocking.     

Lactation performance of spring-calving dairy cows grazing mixed perennial ryegrass/white clover swards of differing composition and height

An experiment was designed to examine the changes in clover content of three mixed perennial ryegrass/white cover swards of differing initial clover contents subjected to different grazing height management regimes and their effect on lactation performance of 48 Friesian dairy cows and heifers. Two paddocks were established for each treatment and grazed on alternate days. Treatments T17 and Tl3 consisted of swards with initial clover contents of 0·17 and 0·13 of the dry matter (DM) mass, respectively, grazed to maintain compressed sward heights of 6 cm throughout the season. A third treatment, SI5, consisted of a sward with an initial clover content of 0·15 grazed to maintain a compressed sward height of 4·5 cm for the first 78 days of the grazing season (period 1). Throughout period 1, half the animals on each treatment each received 4 kg of a concentrate supplement daily, while the others remained unsupplemented. From days 79 to 90, the cattle on treatment S15 grazed a similar sward, while the compressed sward height of the S15 paddocks was allowed to increase to 6 cm before re introduction of the animals. The three swards were then grazed for a further 47 days (period 2) before the animals were housed and milk yield recorded for a further 63 days (period 3). While sward T17 showed little change in clover content over the first 29 days of grazing, remaining at just below 0·18 of DM mass, swards T13 and S15 showed a marked decline in clover content to 0·05 and 0·07 of DM mass respectively. However, by the end of period 1 the clover content of all three swards had increased markedly (0·25, 0·15 and 0·15 of DM mass respectively). By the end of period 2, clover proportions were slightly higher than initial values (0·19. 0·15 and 0·15 of DM mass for treatments T17, T13 and S15, respectively). Owing to the relatively small differences in clover content of swards TI7 and T13, there were no significant effects of these two treatments on milk yield or composition in any period. Supplementation had no effect on milk composition and had little effect on milk yield, except when sward height was maintained at 4·5 cm. There was no carryover effect of supplementation on milk yield or composition in periods 2 or 3.     

Dry-matter yield and herbage quality of a perennial ryegrass/white clover sward in a rotational grazing and cutting system

The expected reduction in the use of fertilizer nitrogen (N) on grassland in the Netherlands has led to renewed interest in white clover. Therefore, the performance of a newly sown perennial ryegrass/white clover sward on clay soil was assessed during 4 consecutive years. The experiment consisted of all combinations of two defoliation systems, i.e. one or two silage cuts per year (S1, S2), spring N application rate, i.e. 0 or 50 kg ha⁻¹ year⁻¹ (N₀, N₅₀), and the management system, i.e. rotational grazing and cutting, or cutting only (RGC, CO). The overall mean white clover cover was 30%. All treatments affected white clover cover, which was 8% higher with S2 than with S1, 6% higher with N₀ than with N₅₀ and 12% higher with CO than with RGC. The overall mean annual dry-matter (DM) yield (13·1 t ha⁻¹ year⁻¹) was significantly affected only by the management system: in two relatively wetter years, the annual DM yield was 1·19 t ha⁻¹ higher with RGC than with CO, whereas there was no difference in two relatively drier years. Nitrogen application increased the DM yield in the first cut by 7·0 kg kg⁻¹ N applied, but had no significant effect on the annual DM yield. Herbage quality was not affected by the experimental treatments. The average in vitro organic matter digestibility was 0.801, and the average crude protein content was 193 g kg⁻¹ DM. With the expected reduction in the use of fertilizer N, perennial ryegrass/white clover swards should be seriously considered as an alternative option to perennial ryegrass swards on these clay soils.     

The influence of fertilizer nitrogen, white clover content and environmental factors on the nitrate content of perennial ryegrass and ryegrass/white clover swards

In a field experiment carried out over 3 years, the nitrate content of herbage from perennial ryegrass (Lolium perenne) swards increased exponentially with nitrogen application rate, but herbage nitrate content appeared to reach potentially dangerous concentrations only when nitrogen application rates were greater than those needed to stimulate dry-matter production. Thus, on average over all the harvests, maximum yield could be obtained with annual application rates of 400 kg N ha^–1 (six applications of 67 kg N ha^–1) for perennial ryegrass and 300 kg N ha^–1 (six applications of 50 kg N ha^–1) for perennial ryegrass/white clover (Trifolium repens) swards, whereas the mean nitrate concentrations were 3340 and 2929 mg NO₃ kg^–1 dry matter (DM) respectively. Nitrate content, however, varied considerably from harvest to harvest, reaching maxima of 9345 mg NO₃ kg^–1 DM at 400 kg N ha^–1 for perennial ryegrass and 6255 mg NO₃ kg^–1 DM at 300 kg N ha^–1 for perennial ryegrass/white clover. The nitrate content of herbage from perennial ryegrass/white clover swards was always greater than that of perennial ryegrass swards receiving the same rate of nitrogen application, even though in the herbage from the mixed sward the nitrate content of white clover was usually less than half that of the perennial ryegrass component. The physical environment did not have a clearly interpretable effect on nitrate content, although herbage harvested in May had a much lower nitrate content than that harvested at any other time of the season. It was not possible to find a single multiple regression equation relating herbage nitrate content to nitrogen application and to other environmental variables that explained more than 60% of the variance in herbage nitrate, but it is suggested that, by reducing the later-season nitrogen applications from 67 to 50 and finally to 33 kg N ha^–1 for perennial ryegrass and from 50 to 33 kg N ha^–1 for perennial ryegrass/white clover, it would be possible to achieve over 90% of the maximum yield while reducing average nitrate content to <40% of that at maximum yield, with no samples containing more than 2300 mg NO₃ kg ^–1 DM.     

The effects of frequency of cutting and cultivar on the period of leaf expansion in white clover grown in mixed swards

The period of leaf expansion in white clover ( Trifolium repens ) grown with perennial ryegrass ( Lolium perenne ) in mixed swards and the relationship between the period of leaf expansion and leaf appearance rate were examined in a field experiment in The Netherlands in which two cutting frequencies (low and high) and three white clover cultivars (Alice, Retor and Gwenda) were used. A significant positive correlation existed between the period of leaf expansion and leaf appearance rate under a low frequency of cutting, indicating that a longer cutting interval might allow the expression of cultivar and environmental differences in both leaf expansion and leaf appearance rate. In most cases, frequent cutting tended to shorten the period of leaf expansion, which may be an important cause of lower dry-matter yield in the high frequency of cutting treatment. The large-leaved cv. Alice had a significantly longer period of leaf expansion than the other cultivars under frequent cutting in autumn. This might partly account for the mixtures with Alice having a greater proportion of white clover than the mixtures with Retor and Gwenda in autumn. Some of the variation between low- and high-frequency cutting treatments in the period of leaf expansion could be explained by mean and minimum temperatures (38% and 15% respectively).     

Effects of cultivar and cutting frequency on dynamics of stolon growth and leaf appearance in white clover grown in mixed swards

The hypothesis that dynamics of growth, branching of stolons and appearance of leaves are important for the persistence of white clover ( Trifolium repens ) in mixed swards was tested. The effect of cutting frequency and white clover cultivar on stolon and leaf dynamics was studied throughout the growing season in a field experiment. Mixtures of perennial ryegrass ( Lolium perenne ) and white clover cultivars with different leaf sizes, cvs. Alice, Gwenda and Retor, were evaluated for white clover persistence at two cutting frequencies. Stolon dynamics, stolon survival, leaf and node appearance rate, branching, flowering and stolon elongation rate were analysed and related to white clover content and yield.
There were clear seasonal fluctuations in stolon and leaf characteristics. Stolon elongation rate and appearance rates of nodes, leaves and branches declined in autumn. Temperature and irradiation explained a major part of the variation of stolon elongation rate and leaf appearance rate and 25% of the variation in stolon branching rate.
Significant and consistent differences in yield and botanical composition were found between mixtures. Mixtures with cv. Alice had the highest total and white clover yield and the highest white clover content, whereas mixtures with cv. Retor had the lowest yield and the lowest white clover content. Stolon elongation rate and stolon internode length were significantly higher in cv. Alice than in the other white clover cultivars, whereas cv. Retor had a significantly higher percentage of leaves with damage caused by insects and slugs than the other cultivars. Little support was found for the hypothesis that branching characteristics are a key component of competitive success in mixed pasture, as most measured branching characteristics were not associated with differences in white clover yield between cultivars and cutting regimes.     

Effect of cultivar and seed rate of perennial ryegrass and strategic fertilizer nitrogen on the productivity of grass/white clover swards

Four cultivars of perennial ryegrass (intermediate diploid cv. Talbot and tetraploid cv. Barlatra, and late diploid cv. Parcour and tetraploid cv. Petra) were each sown at 10,20 and 30 kg ha^-1, all with 3 kg ha^-1 of white clover cv. Donna. Herbage productivity was measured over 3 harvest years, 1982–84. under two annual rates of fertilizer N (0 and 150 kg ha^-1); the 150 kg ha^-1 rate was split equally between March and August applications. Fertilizer N increased total herbage DM production; the 3-year means for the 0 and 150 kg ha^-1 N rates were 8·04 and 8·91 t ha^-1, respectively. In successive years, total herbage responses to N (kg DM (kg N applied)^-1) were 6·6, 35 and 72 (overall mean, 58). Mean white clover DM production over the 3 years was reduced from 4·48 t ha^-1 at nil N to 2·82 t ha^-1 at the 150 kg ha^-1 rate, a fall of 37%. Grass seed rate did not influence total herbage production or white clover performance. The two intermediate perennial ryegrass cultivars had a marginal advantage in total herbage production over the two late cultivars, but white clover content and production were higher with tetraploids than diploids. It is concluded that the value of increased herbage production from strategic use of fertilizer N has to be weighed against its depressive effect on white clover performance; application of 75 kg ha ha^-1 N in both spring and autumn was excessively high if maintenance of a good white clover content in the sward is an objective. There is considerable flexibility in the grass: clover seed ratio in seeds mixtures. Modern highly-productive perennial ryegrass varieties do not differ substantially in compatibility with white clover but tetraploids permit better clover performance than diploids.     

The effect of sward height on responses of mini-swards of perennial ryegrass/white clover to slurry application

The influence of sward height at the time of slurry application on sward responses to slurry was investigated using perennial ryegrass ( Lolium perenne )/white clover ( Trifolium repens ) mini-swards under greenhouse conditions. Pig slurry, cattle slurry and a fertilizer control were applied either to: swards cut 1 d before slurry application to heights of 2, 4 or 8 cm (CH2, CH4 or CH8); or to swards cut to 4 cm with regrowth intervals of 1, 4 or 8 d before slurry application (RI1, RI4 and RI8). Scorch, smother and growth of marked clover stolons and grass tillers were monitored after slurry application. Dry-matter yields of both species were recorded over two harvests. Electrolyte leakage from leaves was used to assess leaf damage. Both slurries increased leakage, which was greatest from clover leaves, with biggest increases caused by pig slurry. Yield responses to slurry application varied with plant species and sward height. Reduced grass growth after slurry application to long swards (CH8, RI4, RI8) was probably caused by greater smother. In short swards (CH2), grass leaf growth was not depressed following slurry application but grass yields were reduced. Ammonia volatilization losses may have been greater in both long and short swards, contributing to yield reductions. Yield responses to slurry were greatest in swards cut to 4 cm, 1 d before application.     

A comparison of diploid and tetraploid perennial ryegrass and tetraploid ryegrass/white clover swards under continuous sheep stocking at controlled sward heights. 2. Animal production

Performance of continuously stocked Mule ewes nursing Suffolk-cross twin lambs over three grazing seasons, between April and August, was compared on swards of N-fertilized diploid perennial ryegrass (D), tetraploid perennial ryegrass (T) and tetraploid perennial ryegrass with white clover (TC), the latter receiving no fertilizer N. Sward height was maintained by variable stocking rate close to a target of 4–6 cm (constant treatment) from turnout and compared in July and August with a rising sward height treatment (target 6–8 cm). Lambs on TC swards had significantly higher (P <0·001) liveweight gains compared with lambs on T swards by 41 gd^-1 in April–June and by 68gd^-1 in July-August. Live weight and body condition score of ewes in August were significantly higher (P<0·001) on TC compared with T swards, by 11·3 kg and 0·75 respectively. Rising sward heights in July–August increased live-weight gain of lambs compared with constant sward heights by 102, 39 and 54gd^-1 in consecutive years, associated with sward height increases of 0·9, 0·5 and 0·6cm respectively. Rising sward height increased ewe live weight and body condition score by 5·1 kg and 0·3 respectively, compared with results from constant sward heights. Effects of sward height and sward type were additive. T swards had a significantly (P<0·01) 16% greater overall lamb output than the D swards due mainly to a 10% higher achieved stocking rate. Stocking rates of ewes on TC vs T swards were 40, 13 and 12% lower in April-August in successive years. The higher liveweight gain of lambs on the TC swards resulted in lamb outputs of 76, 105 and 101% of the T swards in successive years, showing that grass/clover swards containing over 20% clover could produce similar lamb output ha^-1 to grass swards given 150–180 kg N ha^-1.     

The effect of cutting and grazing managements on dry matter yield of white clover varieties (Trifolium repens) when grown with S23 perennial ryegrass

The effects of three defoliation treatments upon the performance of eight white clover varieties when grown with S23 perennial ryegrass were investigated. These treatments included a cutting only regime as used in National List trials and rotational and continuous sheep grazing.
There were significant ( P <0·001) differences between treatments, and the ranking of clover varieties also differed between the three treatments. The significance of this finding is discussed in relation to the evaluation of breeding material and varieties prior to recommendation to farmers.
Clover yields were less under grazing than cutting. This was due mainly to the selective grazing and removal of stolon material which occurred in the former treatments. Differences in the grass/clover balance resulting from the three treatments are discussed in relation to the degree of protection afforded to the clover stolons by the grass component.     

The liveweight gain of Limousin × Friesian heifers grazing perennial ryegrass/white clover swards of different clover content and the effects of their grazing on sward botanical composition

An experiment was carried out in 1992 and 1993 to examine the effect of white clover content of perennial ryegrass/white clover swards on the performance of Limousin × Friesian heifers. Swards with low (L), medium (M) and high (H) white clover contents were established and managed by continuous variable stocking. A compressed sward height of 5·5 cm was maintained using a buffer fence to vary plot areas, with herbage surplus to grazing requirements cut, removed and yields measured. The mean white clover proportions for treatments L, M and H were 0·02, 0·19 and 0·18 in 1992 and 0·13, 0·16 and 0·31 in 1993 respectively. White clover contents of the swards reached a maximum in August and September, and differences between treatments diminished. There was no significant difference between treatments in the content of white clover in the swards in autumn 1993.
Liveweight gains of heifers increased asymptotically with increasing white clover content of the sward. Below a white clover herbage mass of 300 kg DM ha^–1, there was little effect on liveweight gain, which was 0·70 kg day^–1 over the grazing season. Between 400 and 450 kg DM ha^–1 white clover, liveweight gains were 0·85–0·90 kg day^–1. While clover content of the sward did not significantly affect utilized metabolizable energy output; the mean output over the grazing season in the two years from liveweight gain and herbage yield was 78 GJ ha^–1. It is suggested that, using this grazing system, white clover reached an equilibrium with a mean herbage mass of about 400 kg DM ha^–1 over the grazing season.     

The response of mini-swards of perennial ryegrass/white clover to simulated rainfall following slurry application

Perennial ryegrass/white clover mini-swards were used lo determine whether sward responses to slurry were modified by subsequent simulated rainfall (SR). Combinations of two SR volume rates (6.5 and 13 mm), three SR timings (3, 24 and 48 h after slurry application) and two slurry types (cattle, pig) were compared with a fertilizer control (no SR). Scorch, smother and growth of marked stolons and tillers were monitored after slurry application, and yields of both species were recorded. Slurry coverage of plastic squares indicated that smother may be greater on horizontally orientated leaves (clover). Reduced grass leaf growth and numbers of clover growing points in the week following slurry application were attributed mainly to smother, and possibly other phytotoxic effects of slurry. Reasons for the superior clover growth with cattle slurry arc unknown, although its higher P and K content may have contributed. Timing of rainfall is important in influencing sward responses to slurry application. Results suggest that application of slurry may be most beneficial when it immediately precedes rainfall and that longer intervals between slurry application and rainfall reduce grass and clover growth through either greater ammonia loss or slurry negative effects.     

The effects of sward height and nitrogen fertilizer application on changes in sward composition, white clover growth and the stock carrying capacity of an upland perennial ryegrass/white clover sward grazed by sheep for four years

Perennial ryegrass/white clover pastures grazed by sheep and receiving either no fertilizer N (No) or 120 kg N ha^?1 year^?1 (N₀) were maintained with surface heights of 2·5, 3·5 and 5·0 cm for over four years. The treatments were replicated. The white clover (WC) population was greatest in the N₀treatment, and declined during the study. Between-year variation in WC was negatively related to rainfall and positively related to temperature, WC as a proportion of the total plant population decreased during the summer in the Nl treatment. The perennial ryegrass (PRG) population was greater in the Nl treatment, declined during the study and both within and between years was positively related to temperature. The population density of the unsown grasses was highest in the N-fertilized treatment and in the swards maintained at the lowest heights (these treatments also had the highest stocking rate); it increased during the study, within-years being positively related to temperature and between-years being positively related to rainfall. The WC stolon extension rate was largely unaffected by N fertilizer application and was greatest in the taller swards. Leaf appearance rate was unaffected by N fertilizer application and sward height; it was positively related to temperature and negatively related to rainfall. Branching rate was greater in the N₀ treatment with significant sward height effects confined to a negative relationship with local sward height within treatment plots on one occasion; it was negatively related to rainfall. The ground level red:far red light ratio was negatively related to local sward height. The total live weight of sheep carried in the No treatments was 0·7 of that in the N₁ treatments. Expected photomorphogenic responses by we were confined to stolon extension. It was concluded that on the poorly drained clay-loam soil used in this study the effects of sheep, in interaction with climatic factors, had an overriding effect on clover branching rate and the ultimate species composition.     

A comparison of diploid and tetraploid perennial ryegrass and tetraploid ryegrass/white clover swards under continuous sheep stocking at controlled sward heights. 3. Sward characteristics and animal output, years 4–8

Results for years 4–8 of a long-term grazing experiment on swards of a diploid perennial ryegrass (Lolium perenne), var. Contender (D swards), a tetraploid ryegrass, var. Condesa (T swards) and Condesa with S184 white clover (Trifolium repens) (TC swards), direct sown in May 1987, are presented. The swards were continuously stocked with sheep from 1988 to 1990, as previously reported, and for a further 5 years, 1991–95, at a target sward surface height (SSH) of 4–6 cm. Control of sward height was successfully achieved by variable stocking, except in 1993 when paddocks were set stocked and the resulting mean SSH was 9·3 cm. Grass swards received on average 160 kg N ha^?1 year^?1; grass/clover swards were mainly not fertilized with N with the exception that they were given 30 kg N ha^?1 as a remedial mid-summer application during a period of low herbage mass on offer in 1994 and 1995. Mean white clover content of the swards fell from 18·2% of herbage dry-matter (DM) in 1992 to 8·5% in 1993, whereas stolon lengths fell from 120 to 58 m m^?2. A return to lower sward heights in 1994–95 resulted in an increase in white clover content to 12·8% by the final sampling in August 1995. Perennial ryegrass content of the grass swards remained high throughout (mean 96·7% in 1995). Perennial ryegrass tiller densities recorded in August 1991, 1993 and 1994 showed consistently significant (P < 0·001) sward differences (3-year mean 16 600, 13 700 and 10 100 perennial ryegrass tillers m^?2 for the D, T and TC swards). In 1994, the year after lax grazing, a low perennial ryegrass tiller density (9100 m^?2) and low white clover content (mean 4·3%) in the TC swards resulted in a much lower herbage bulk density than in the grass swards (April–July means 72, 94 and 44 kg OM ha^?1 cm^?1 for the D, T and TC swards). There was a consistent 40 g d^?1 increase in lamb liveweight gain on the TC swards over the T swards, except in 1994. In that year there was a reduction in lamb liveweight gain of 33 g d^?1 on the TC swards and a significant increase in ewe liveweight loss (117 g d^?1) associated with low herbage bulk density despite optimal sward height. Lamb output (kg liveweight ha^?1) on TC swards reflected white clover content, falling from a similar output to that produced from grass given 160 kg N ha^?1, at 18% white clover DM content, down to 60% of grass + N swards with around 5% clover. A 6% greater output from the T than the D swards was achieved mainly through higher stocking rate. The experiment demonstrated a rapid, loss in white clover under lax grazing, and showed that the relationship between performance and sward height is also dependent on herbage density. High lamb output from a grass/clover sward was only achieved when the clover content was maintained at 15–20% of the herbage DM.     

Interactions between pesticide treatment, cutting frequency and rate of N fertilizer on white clover grown in mixture with perennial ryegrass

The effects of a combined pesticide and fungicide (P + F) treatment on the proportion of white clover in an established perennial ryegrass/white clover sward were assessed on six occasions during 1988 and 1989, under two cutting frequencies (2- or 6-week intervals) and with or without N fertilizer (120 kg ha^-1 per annum). In 1988 P + F treatment significantly increased the proportion of clover in August and October, with an increase at the latter harvest being much greater where N was also applied. There was no significant interaction between P + F treatment and cutting frequency, although less frequent cutting increased significantly the proportion of clover in October where N was not applied.
In 1989, drought prevented the proportion of clover from exceeding 1% throughout, regardless of management factors. P + F treatment significantly increased the proportion of clover in August, and significantly reduced populations of slugs, foliar-feeding insects (mainly Collembola; Sminthuridae) and the area of clover leaf damaged by pests. No marked effect of P + F treatment on populations of plant parasitic nematodes was detected. No appreciable incidence of fungal diseases was observed in either year.     

Soil N contributes to the oscillations of the white clover content in mixed swards of perennial ryegrass under conditions that simulate grazing over five years

The effect of the initial N-supplying capacity of soils (SoilN, 90–230 kg N ha^–1 year^–1) was tested on the dry-matter and N yields of pure or mixed white clover and perennial ryegrass swards, managed under simulated grazing over a 5-year period. The cumulated N harvested in the mixed swards was similar, both for white clover and perennial ryegrass, but the proportion of white clover showed oscillations over a 2-year period. In the first year, the SoilN effect was similar to that of fertilizer N. During the course of the experiment, the effect was always positive on the pure perennial ryegrass sward, alternately negative and nil for the white clover in the mixed sward and alternately positive and nil for the perennial ryegrass in the mixed sward; the period of these oscillations was 2 years. From the third regrowth period after sowing, the ratio between the actual N concentration and the concentration non-limiting to growth for the perennial ryegrass in the mixed sward, increased above that of the pure perennial ryegrass sward. It was in turn greater in the soils that were initially poor and then greater in those that were initially rich in soil N. The periodic oscillation of the initial SoilN effects implies that the initial SoilN gradient was alternately compensated and restored. It was concluded that N fluxes are partly responsible for the temporal oscillations in the proportion of white clover in mixed swards.