Contamination of grape seed oil with mineral oil paraffins

The contamination of 11 commercial grape seed oils with paraffins of mineral oil origin was analyzed by online-coupled HPLC-HPLC-GC-FID and ranged from 43 to 247 mg kg(-1). The analysis of the marc and seeds indicated that the contamination is primarily from the peels. Since superficial extraction of the seeds with hexane removed most of the mineral paraffins, the contamination of the seeds is largely on the surface, perhaps transferred from the peels during storage of the marc. Mechanical purification of the seeds combined with washing with hexane reduced the contamination of the oil by a factor of about 10. The refining process removed 30% of the mineral paraffins, primarily the more volatile components. Oil obtained from the seeds of fresh grapes, including grapes not having undergone any phytochemical treatment, contained clearly less mineral paraffins (up to 14 mg kg(-1)), and the peels were less contaminated, suggesting an environmental background contamination. To this an additional contamination might be added by a treatment of the grapes used for wine making.     

~(137)Cs在土壤中的污染行为与钾盐的防治效果

本研究结果表明 ,在污染水平相同的情况下 ,不同土壤对春小麦吸收137Cs有很大影响 ,植株中137Cs的比活度相差几十倍。在同一种土壤、不同污染水平下 ,春小麦对137Cs的吸收与土壤中137Cs的污染水平呈正相关。在137Cs污染的土壤上施用钾盐可以降低春小麦植株对137Cs的吸收。防治效果与钾盐施用量有关 ,本试验结果为土壤阳离子代换量 1 / 3 0的量施用效果最好 ,使植株中的137Cs比活度降低 84 74%～88 89% ,与不施钾盐的对照组的差异十分显著     

Drying of Pedro Ximenez grapes in chamber at controlled temperature and with dipping pretreatments. Changes in the color fraction

The drying of Pedro Ximenez grapes in chamber at a controlled temperature of 40 or 50 degrees C is studied. Compared to traditional sun-drying, the chamber-drying shortened the drying time by about 40% at 50 degrees C. In color terms, the musts obtained from grapes dried at 50 degrees C were closer in CIELab coordinates to those obtained by sun-dried grapes, with similar h(ab) values and slightly lower L* and C(ab)*. To shorten further the drying times at 50 degrees C, the grapes were dipped in olive oil or ethyl oleate emulsions containing potassium carbonate. The ethyl oleate pretreatment shortened additionally the drying time by about 25%, providing musts with chroma, lightness, and hue similar to those without grape pretreatment. In general, except for the phenolic compounds corresponding to the drying with ethyl oleate pretreatment, most of these compounds in the remainding conditions studied increased to a lesser extent than expected because of water losses of the grapes during drying, revealing degradation reactions.     

Fungal flora and ochratoxin a production in grapes and musts from france

Eleven samples of grapes and musts used in red table wines were investigated for the occurrence of potential ochratoxin A (OTA)-producing molds. From these samples, 59 filamentous fungi and 2 yeasts were isolated. Among the 30 genera isolated, Deuteromycetes were the most frequent (70%) followed by Ascomycetes (10%). Six of the eleven grapes samples were contaminated by potentially ochratoxinogenic strains (Penicillium chrysogenum and Aspergillus carbonarius). When cultivated in vitro on solid complex media, the 14 strains of A. carbonarius produced OTA. No other species produced OTA under the same conditions. Among must samples, eight of eleven were found to be contaminated by OTA (concentrations from <10 to 461 ng/L). There is a strong correlation between the presence of ochratoxin-producing strains on grapes and OTA in musts. These findings should be connected with the OTA contamination of human blood in these areas and in France.     

Ultraviolet-C and induced stilbenes control ochratoxigenic Aspergillus in grapes

This study investigated the efficacy of ultraviolet-C (254 nm) and induced stilbenes to inhibit Aspergillus carbonarius and Aspergillus tubingensis and control ochratoxin A production in grapes. In addition, the stilbene synthesis as a response to UV-C treatment and to infection of ochratoxigenic Aspergillus was compared. The initial microbial inactivation by a previously optimized UV-C illumination protocol for increasing trans-resveratrol content in grapes (50 W/m (2), 40 cm, 60 s) was similar on undamaged and damaged grapes, achieving 1.2 and 1.3 log conidia/100 g reductions, respectively. After 5 days of storage at 22 degrees C, UV-C treatment and the stilbenes induced by UV-C inhibited ochratoxigenic Aspergillus growth in undamaged grapes. UV-C elicited the biosynthesis of trans-resveratrol, while microbial infection and tissue damage triggered the biosynthesis of trans-piceid. trans-Resveratrol was not synthesized as a consequence of ochratoxigenic Aspergillus contamination. However, when trans-resveratrol was synthesized by UV-C, it contributed to inhibiting the development of ochratoxin A producing aspergilli. Furthermore, UV-C treatment also contributed to decrease ochratoxin A production by ochratoxigenic aspergilli. Therefore, UV-C is a promising emerging technology either for reducing the potential ochratoxigenic risk in grapes, which is of particular interest to the wine industry, and also for increasing trans-resveratrol content of grapes, which would provide an added value to the wine.     

Improving grape phenolic content and wine chromatic characteristics through the use of two different elicitors: methyl jasmonate versus benzothiadiazole

Benzothiadiazole (BTH) and methyl jasmonate (MeJ) have been described as exogenous elicitors of some plant defense compounds, polyphenols among them. The objective of this study was to determine whether the application of BTH or MeJ to grape clusters at the beginning of the ripening process had any effect on the accumulation of the main flavonoid compounds in grapes (anthocyanins, flavonols, and flavanols) and the technological significance of these treatments in the resulting wines. The results obtained after a 2 year experiment indicated that both treatments increased the anthocyanin, flavonol, and proanthocyanidin content of grapes. The wines obtained from the treated grapes showed higher color intensity and total phenolic content than the wines made from control grapes. The exogenous application of these elicitors, as a complement to fungicide treatments, could be an interesting strategy for vine protection, increasing, at the same time, the phenolic content of the grapes and the resulting wines.     

日本水稻核放射性污染现状及去污措施综述

2011年3月日本福岛核电站发生核泄漏事故,释放出大量放射性物质,使周边农田受到直接污染。其中对水稻影响最为严重的放射性核素为放射性铯。分析表明:水稻对铯的吸收是存在差异的,钾肥能有效降低水稻中铯含量,而氮肥会增加其铯含量;距离福岛核电站距离越远其受污染程度越低;土壤中铯的浓度、含钾量、固定铯的能力和耕作表土的厚度都影响着铯的转移;此外,水稻本身的品种也影响着对铯的吸收。水稻去污染的方法包括:利用蓝藻除去表土中放射性铯、对土壤进行深耕及防止水田二次污染等方式来降低土壤本身铯含量;另一方面通过改变土壤粒度分布,向土壤中加入施威特曼石、沸石和蛭石及控制肥料比例等方式来抑制土壤中铯的转移。以上除污染方法的综合应用效果十分显著,福岛县水稻含铯量于2014年已全部低于25 Bq/kg。该研究为中国构建农作物放射性污染特征数据库及相关应急管理提供参考。     

基于作物水分亏缺指数的环渤海地区鲜食葡萄干旱风险评估

水分对葡萄的生长发育过程有着不容忽视的影响。为了给鲜食葡萄种植干旱风险管理和减损保质提供一定理论参考,本研究以中国葡萄环渤海产区为研究区域,以作物水分亏缺指数为探究鲜食葡萄干旱的指标,采用区域内60个气象站点1981−2014年的逐日气象资料,通过计算区域内鲜食葡萄各生育期内的作物水分亏缺指数,得到各生育期鲜食葡萄的干旱频率及站次比,基于区域内鲜食葡萄干旱时空分布情况评估其干旱发生风险。结果表明：环渤海地区降水量与鲜食葡萄需水量的时间匹配性较差,区域内鲜食葡萄在各个生育阶段均具有一定的干旱风险;以鲜食葡萄的新梢生长期及着色成熟期为发生干旱的高风险时期,该时期发生干旱频率高且易发生较为严重的干旱;以河北省中南部为干旱高风险地区,该地区鲜食葡萄发生严重干旱风险较高,且环渤海地区鲜食葡萄以着色成熟期干旱影响范围最广。     

施钾对茶园土壤钾素及平衡的影响

盆栽选取四川、重庆典型的砂岩黄壤、灰岩黄壤、酸性紫色土为供试土壤,田间选取在砂岩黄壤上生长了25年的茶园,连续5年研究了施用钾肥(硫酸钾)对茶园土壤钾库及钾素平衡的影响。结果表明:施用钾肥能显著提高土壤钾库的各类钾素,但其绝对含量都呈逐年下降的趋势。土壤中各类钾素的变化因土壤类型而异,土壤各类钾素的增量均为灰岩黄壤砂岩黄壤酸性紫色土。不施钾肥(CK)的盆栽茶树、田间茶树分别每年从土壤取走钾素0.10.g/kg。盆栽三种土壤的钾素年平均流失量分则别为0.18.g/kg(灰岩黄壤)、0.09.g/kg(砂岩黄壤)、0.07g/kg(酸性紫色土),可见种植茶树施用K肥很有必要。     

野外Lysimeter试验^14C同位素示踪研究土壤-小麦系统多环芳烃萘的环境归趋

采用^14C同位素示踪技术和野外Lysimeter试验,进行了小麦种植条件下多环芳烃萘（naphthalene）的环境归趋的定量化研究。结果表明,气态损耗是土壤中外源萘含量减少的主要途径,其中矿化损失约占气态损耗的84．41％,作物的存在可显著促进萘的气态损耗,矿化速率和挥发速率可分别提高43．51％和190．32％,其原因可能与植物的根系分泌活动有关。经过一个小麦生长周期,土壤表层残留^14C占施入量的1．73％;对土壤柱样的分层分析和土壤渗滤放射性强度测定发现,萘存在明显的纵向迁移现象并可能影响地下水水质,渗滤损失约占施入总量的0．043％;收获期萘在小麦体内含量仅占外源施入量的0．003％,但小麦籽粒已明显检测到^14C的存在,浓度约为0．16mg·kg^-1,因此农田环境中多环芳烃残留对食品安全的影响也应引起重视。     

~(137)Cs不同污染水平在大亚湾、秦山、北京土壤植物系统的转移

采用我国大亚湾、秦山和北京土壤 ,模拟核裂变产物137Cs在 0Bq kg土、3 3×1 0 4 Bq kg土、3 3× 1 0 5Bq kg土、3 3× 1 0 6 Bq kg土污染水平时 ,研究土壤 -小麦系统的污染影响与137Cs核素的转移规律。结果表明 :在本试验条件下小麦生长发育处于正常状态 ,未发现对小麦有不良影响 ;小麦分别在 3个污染水平下从试验用的 3种土壤中吸收137Cs的趋势是一致的 ;小麦植株中137Cs的比活度随137Cs施入量相应以数量级增加 ,两者间呈十分显著的正相关 ;137Cs在土壤 -小麦系统中的转移系数随土壤的性质不同而变化 ,在同一污染水平下转移系数在n× 1 0 - 2 ～n× 1 0 0 范围变化 ;在同一种土壤中 ,随土壤污染水平的提高 ,转移系数亦有所提高 ,但变化范围不大 ,对北京土转移系数为 2 2× 1 0 - 2 ～ 5 4× 1 0 - 2 ,大亚湾土转移系数为 1 0 4～ 2 0 9,均在同一数量级内变化 ;对秦山土转移系数为 0 51～ 1 2 1。     

木质素改性产物对钾肥的缓释作用与作物吸钾量的影响

利用木质素改性产物作为肥料添加剂 ,与氯化钾复混制成木质素钾肥 ,通过土柱淋溶与盆栽试验 ,验证木质素对钾肥的缓释作用与作物对钾肥利用情况。结果表明 ,木质素不仅可以减少钾肥流失 ,而且可以促进番茄与玉米生长 ,提高作物对钾的吸收。木质素来源于造纸废液 ,作为钾肥的缓释材料 ,其资源环保意义重大。     

Comparison of the anthocyanin composition during ripening of Syrah grapes grown using organic or conventional agricultural practices

The anthocyanin composition of Syrah grapes harvested at different stages of ripening and produced using organic or conventional agriculture was studied. Samples of grapes were collected from veraison to full maturity in each plot, and the content in nine anthocyanins was determined by high-performance liquid chromatography with diode array detection. The total content in anthocyanins during ripening of the conventionally grown grapes was significantly higher compared to that found in the organic production. The accumulation of anthocyanins reached a maximum 28 days after veraison (in agreement with high temperature) and then decreased until harvest. In all samples, grapes from the conventional agriculture presented higher proportions of delphinidin, petunidin, malvidin, and acylated malvidin glucosides compared to grapes from organic agriculture. In contrast with other comparative studies of organically and conventionally grown plants, the results demonstrated a higher content in anthocyanins in conventionally grown grapes.     

Modalités de la contamination de deux chaînes trophiques dulçaquicoles par le cobalt 60

Introduction

A previous work (Amiard-Triquet and Foulquier, 1978) has covered the dynamics of water-borne contamination in organisms included into two freshwater food-chains: Here, we report the results obtained about the contamination of the same species bothvia water and food.

Experimental Procedures (Table I)

The specimens were continuously immersed in contaminated water and received radioactive food. The radioactivity level was the same for the water in which they were immersed and for the water used in the contamination of their prey. The level was adjusted at 4 μCi/l for the carp food-chain and 0.4 μCi/l for that of the crayfish. Daphnia were reared in a medium compo?ed of 2/5 of soil extract and 3/5 of river water (Table II). They were fed bacteria developed in this medium or uponChlorella cultivated apart and then introduced in the rearing tanks. The bulk of the medium varied between 5 and 30 l. The influence of the various components of the rearing medium on the physico-chemical form of⁶⁰Co was studied by chromatographic techniques using ion-exchange resins. Three groups of fiftyLymnaea were placed in three tanks containing 30 l of river water.Elodea which composed their food were immersed in the same tanks. Crayfishes and carps were reared in individual containers. Five times per week, they received, respectively, oneLymnaea or one hundredDaphnia. Water was changed and contaminated again each week.

Results

NH ₄ ⁺ ions produced in the rearing medium with organic metabolites extracted byDaphnia do not affect the physico-chemical form of⁶⁰Co. On the contrary, anionic complex forms appear under the influence of river water or soil extract components. The radioactivity ofDaphnia evolves during the contamination experiment as a function of the biological availability of the different physico-chemical states of cobalt. The maximum value of the overall contamination factor C^* is 70.3 after one week of experiment; the minimum value, 8.6 after ten months. The contamination dynamics for the other species are presented in Figures 3 and 4. The mean values at equilibrium for⁶⁰Co concentration in the organisms and for overall contamination factor C are assembled in Table III.

Discussion and Conclusions

Daphnia likely discriminate the different physico-chemical forms of⁶⁰Co, the availability of anionic complexes being low. The vector of contamination, water alone or both food and water, has not a striking influence upon the⁶⁰Co concentration in carp,Lymnaea and crayfish. The most highly developed species from the zoological point of view are the weakest in this concentration: Teleosts are not so contaminated as Crustaceans which are not so contaminated as Mollusks. On the other hand, the secondary consumers (carp, crayfish) are not so contaminated as the primary consumers (Lymnaea, Daphnia) which are not so contaminated as primary producers (Table III). Laboratory experiments andin situ measurements in seawater and freshwater environments reported in the literature confirm a general tendency to a decreasing of radiocobalt concentrations as a function of trophic level.     

快速脱水抑制葡萄干制过程中膜脂过氧化及褐变

为探索脱水速度对无核白葡萄干制过程中膜脂过氧化作用及褐变的影响,以新疆无核白葡萄为试验材料,经过葡萄促干剂处理后,采用快速脱水和缓慢脱水2种处理,质量每减轻10%进行取样,测定脱水过程中果实干基含水率、干燥速率、褐变度、细胞膜透性、丙二醛(malonaldehyde,MDA)含量、脂氧合酶(lipoxygenase,LOX)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、过氧化物酶(peroxidase,POD)、多酚氧化酶(polyphenol oxidase,PPO)活性以及总酚含量的变化。结果表明:与缓慢脱水相比,快速脱水处理显著(P0.05)降低了无核白葡萄褐变度的上升,减少MDA生成量以及膜透性的增加,抑制LOX活性的升高,保持较高的活性氧清除酶SOD、CAT及POD活性及较高的总酚含量,且使PPO活性保持在一个较低的水平。因此认为,快速脱水可以有效地抑制无核白葡萄的膜脂过氧化作用对细胞膜的破坏,保持细胞的完整性,且PPO活性较低,从而减少无核白葡萄脱水褐变的发生。研究结果为快速脱水在无核白葡萄干制中的应用提供参考。     

Vineyard and fermentation studies to elucidate the origin of 1,8-cineole in Australian red wine

Preliminary investigations revealed that the proximity of Eucalyptus trees to grapevines can directly influence the concentration of the aroma compound 1,8-cineole present in the corresponding red wines. For two different vineyards, the closer the grapevines were to the trees, the greater was the amount of 1,8-cineole in the wines elaborated from those grapes. This led us to carry out further studies to quantify the levels of 1,8-cineole found in grape berries, leaves, and stems at set distances from Eucalyptus trees over multiple vintages. Generally, the highest concentration of 1,8-cineole was found in the grapevine leaves, followed by grape stems and then grapes. In each sample type, we observed greater concentrations of 1,8-cineole in samples closer to the trees. Various fermentation treatments carried out with Shiraz grapes showed that matter other than grapes (MOG, e.g., Eucalyptus or grape leaves) could contribute significant amounts of 1,8-cineole to the finished wines. These studies confirmed that vineyard position and winemaking conditions can determine the 1,8-cineole concentration in red wine. The fermentation study also showed for the first time that the concentration of rotundone in red wine can be strongly influenced by grapevine leaves and stems in the ferment.     

Residues of spiroxamine in grapes following field application and their fate from vine to wine

Dissipation of the fungicide spiroxamine in grapes of two vine varieties, Roditis and Cabernet Sauvignon, exposed to field treatments was evaluated. Vines of a grape vineyard located in central Greece were sprayed once or twice with a commercial formulation of the fungicide at 30 g a.i./hL. Residues in grapes, must, and wine were determined by gas chromatography/IT-MS after extraction with cyclohexane-dichloromethane (9:1), with a limit of quantitation 0.02 mg/kg in grapes and 0.012 mg/kg in wine. Under field conditions, spiroxamine dissipation on grapes was faster during the first 2 weeks and then slower to the sixth week. About 7 days after application, half of the initial spiroxamine concentration remained on the grapes; the respective proportion at 42 days was about 10%. At 14 and 35 days, residues were lower than 0.44 and 0.22 mg/kg, respectively, values below the maximum residue levels set by the European Union (1 mg/kg). Spiroxamine residues transferred from grapes into the must and through the vinification process into the wine were also studied. Mean transfer factors of 0.26 and 0.55 were found from grapes into wine for the wines obtained without maceration and with maceration, respectively. Residues in wine, prepared from grapes with a spiroxamine content of 0.11-0.20 mg/kg, varied from <0.026 to 0.09 mg/kg. Spiroxamine diastereomer B was found to dissipate slower than diastereomer A in the field as well as during the vinification process.     

柔性梳脱式酿酒葡萄脱粒机构设计与试验

针对目前中国酿酒葡萄人工采摘效率低下的问题,为实现酿酒葡萄机械化采收,提出柔性梳脱式采摘方式,设计了以柔性弓齿为核心部件的酿酒葡萄柔性梳脱式采摘机构。首先基于ADAMS软件建立了弓齿及果梗的柔性仿真模型。采用广义力建立连接力,传感器监测断裂条件,实现了葡萄果粒在外力作用下的脱粒过程控制,解决了面对多对象脱粒仿真过程控制的关键问题。分析了梳脱辊转速、柔性弓齿弯曲刚度和梳脱辊间距对葡萄脱粒过程的影响规律和机理。基于仿真试验选取因素及水平,并以脱粒时间和破损率为指标,进行了正交试验,确定试验因素最佳组合为:梳脱辊转速160 r/min,弓齿弯曲刚度0.067 N·m2,梳脱辊间距280 mm,在该条件下脱粒时间为1.73 s,破损率为3.51%。该文为深入研究收获过程的机理和收获机构的优化提供参考。     

Wheat yield and leachability of phosphorus and mineral nitrogen in pig slurry amended Soils

Abstract

An outdoor pot experiment was conducted using wheat plants (Triticum aestivum L. cv. Lotti). Each pot was filled with the upper layer of either a Cambic arenosol (soil A) or a Dystric cambisol (soil B) removed from the correspondent lysimeter where a two‐year wheat experiment (1993–1995) had been carried out with an equivalent experimental design as in the present study. The main objectives of this research were (1) to study the effects of increasing applications (5, 15, and 25 t ha^‐1) of solid phase from pig slurry (SP) on wheat yield and tissue elemental composition and (2) to investigate the risk of contamination of drainage water by leaching of phosphorus (P) and mineral nitrogen (N_min) as a result of those applications to the soil. The control consisted of a basic dressing of NPK fertilizer. Results from the study showed that grain production was lower in the 5 and 15 t ha^‐1 SP treatments compared to the control in soil A probably due to a potassium (K) deficiency. Increasing application rates of SP significantly enhanced P and N_min leaching losses in both soils. Finally on soil A high rates of SP amendments led to the contamination of water with P.     

Lead contamination in Portuguese red wines from the Douro region: from the vineyard to the final product

To quantify lead contamination in wines and to try to identify major lead sources, two winemaking processes were followed during one annual cycle of wine production. Two vineyards from the Douro Portuguese region and two types of wine, one red table wine, which has been produced in a very modern winery, and one red fortified wine (similar to Port), which has been produced by a traditional vinification process, were selected for this study. Aerosols from the vineyards atmosphere, vineyard soil, vine leaves, grapes, and samples from the intermediary and final wine product were collected. Suitable pretreatments, namely, high-pressure microwave assisted digestion (soil, leaves, and grapes) and UV-irradiation (grape juices and samples from the different steps of the vinification processes), were used. The samples were analyzed in terms of lead total concentration and respective isotope ratios by using inductively coupled plasma mass spectrometry and atomic absorption spectrophotometry with electrothermal atomization. It was observed that the major sources of lead were in the vinification system, the more traditional one introducing more lead than the modern one. For the fortified wine, the lead concentration increased from 4.7 microg L(-1), in the grape juice, to 17.2 microg L(-1), in the final product, while for the table wine the increase was from 4.1 to 13.1 microg L(-)(1). Therefore, only about 1/4 (fortified wine) and 1/3 (table wine) of the lead total content of the final products came from soil and atmospheric deposition. Therefore, it is expected that marked reductions of the lead content in the wines would occur if the sources of lead were removed from the tubes and containers used in the vinification system, particularly by using welding alloys and small fittings free of lead. The lead levels in the vine leaves (global mean of 0.43 microg g(dry leave)(-1)) and grapes (global mean of 35 ng g(dry grape)(-1)) were similar in both vineyards.