Root Exudates of Rice Cultivars Affect Rhizospheric Phosphorus Dynamics in Soils with Different Phosphorus Statuses

Exudation of organic acids by the roots of three rice cultivars grown in three soils of different phosphorus (P) statuses, and their impacts on the rhizospheric P dynamics and P uptake by the rice plants, were investigated. Quantum root exudates from all the rice cultivars were significantly greater at 21 days after transplantation than at panicle initiation or flowering stages. Malic acid was the most predominant organic acid present in the rice root exudates (10.3 to 89.5 μmol plant^?1 d^?1), followed by tartaric, citric, and acetic acids. Greater exudation of organic acids from rice grown in P-deficient soil by all the rice cultivars suggested response of rice plant to P stress. Results indicate that the release of organic acids in the root exudates of rice plants can extract P from strongly adsorbed soil P fraction, thereby increasing native soil P utilization efficiency and ensuring adequate P nutrition for the growing rice plants.     

Mobilization of phosphorus contributes to positive rotational effects of leguminous cover crops on maize grown on soils from northern Nigeria

Previous research has demonstrated a positive rotational effect of tropical leguminous cover crops on maize growth on a luvisol from Nigeria. This effect could not be explained by a better N supply. The objective of the present work was to further clarify whether improved P nutrition has been a contributing factor. Nine cover crops and maize were studied in nutrient solution‐culture with 1 and 20 μM P and with NO₃‐N as N source for root physiological parameters that may affect P mobilization. Zea mays, Lablab purpureus, and Centrosema pubescens responded to P deficiency by higher rates of proton excretion. Clitoria ternatea excreted OH^— with only small differences due to P nutrition. At low P supply, Chamaecrista rotundifolia, Clitoria, and Centrosema had the highest exudation rates of organic acid anions, especially citrate and malate. A major difference between plant species was found in root‐surface acid phosphatase activity. Cajanus cajan expressed the highest phosphatase activity. V_max of P uptake increased markedly under P deficiency, particularly for maize. Compared to the other plant species Cajanus, Chamaecrista, and Clitoria were characterized by a greater capability to absorb P at low external P concentrations. The nine cover crops and maize were also grown in pot experiments using two soils from northern Nigeria low in available P. The ultisol from Jos had a high P fixation capacity and was more acidic than the alfisol from Zaria. All plant species were precultured in the first season at 100 (Zaria) and 250 (Jos) mg P per pot. In the subsequent season, maize was uniformly seeded into all pots containing the remaining roots and the incorporated shoot dry mass (according to 15 mg P per pot) of the pre‐crop. No P and 250 (Zaria) or 500 (Jos) mg P per pot were freshly applied. Maize growth and P uptake were enhanced after legumes in Zaria soil. Cajanus showed the highest residual effect, and also Clitoria, Chamaecrista, and Lablab showed effects superior to the mean. In Jos soil, Clitoria, Cajanus, and Lablab enhanced maize growth above average. Also, a highly significant positive correlation between P uptake and biomass production was obtained. These results indicate that enhancement of maize growth after leguminous cover crops, at the low P supply of the soil used, mainly depends on P mobilization capacity of the cover crop.     

Variation in Phosphorus Uptake and Use Efficiencies Between Pigeonpea Genotypes and Cowpea

ABSTRACT

Low bioavailability of soil phosphorus (P) often limits N₂ (nitrogen) fixation and crop production in large parts of the tropics. The efficiency of P acquisition and P use by 21 genotypes of pigeonpea [Cajanus cajan (L.) Millsp.] were studied in a pot experiment using two cowpea [Vigna unguiculata (L.) Waip.] genotypes as controls. The short-duration genotypes produced more dry matter, accumulated more P, and produced more dry matter per unit of absorbed P than the medium-and long-duration genotypes. Dry-matter production correlated positively with the P uptake (r² = 0.72) and P-use efficiency (r² = 0.86). The P uptake correlated positively with the P-use efficiency (r² = 0.36); whereas the P-use efficiency correlated negatively with the P-uptake efficiency (r² = 0.50). Root surface did not determine P acquisition of pigeonpea, which absorbed 1.6 mg P cm^?3 from 33 cm³ soil compared with 0.17 mg P cm^?3 from 387 cm³ soil for cowpea at 66 d.     

The quantitative effect of chemical phosphate mobilization by carboxylate anions on P uptake by a single root. II. The importance of soil and plant parameters for uptake of mobilized P

Model calculations were made in order to quantify the effect of carboxylate excretion on phosphate (P) uptake by a single root. The uptake of chemically mobilized P increased exponentially with increasing concentration of adsorbed citrate or oxalate in soil because of the exponential relationship between adsorbed carboxylate and the solubilizing effect of carboxylate on P. The effect of local citrate excretion compared with uniform citrate excretion along the whole root was also calculated. Local exudation increased the uptake of chemically mobilized P because the higher concentration of citrate increases the solubilization of P. Additionally the effect of citrate excretion by root clusters e.g. proteoid roots was evaluated. Uptake of chemically mobilized P by root clusters was much higher than that of single roots, especially if the ratio of P buffering to citrate buffering was high. This is often the case in P fixing soils where by definition P buffering is high and citrate buffering is low because of the short time of reaction between root excreted citrate and rhizosphere soil. The reason for the superiority of cluster roots lies in the fact that most of the mobilized P is transported away from a single root to be absorbed by neighbouring roots in the clusters. This phenomenon demonstrates the strong ecological significance of cluster roots in relation to nutrient mobilization. The calculations on the effect of oxalate excretion by sugar beet roots on the uptake of mobilized P show that under P fixing conditions the influx of mobilized P will exceed that of P transported by diffusion to the root surface by a factor of 1.5—6.0.     

生物炭和堆肥提高西澳大利亚沙质平原土壤上的作物产量但这种效应是短期的

Sandplain soils on the south coast of Western Australia have low inherent fertility, which is mainly due to poor nutrient retention caused by insufficient clay and organic colloidal material. Previous research has shown the benefits in nutrient levels and retention from adding clay to sandplain soils; however, there is almost no information on the addition of organic amendments. A field experiment was established at the Esperance Downs Research Station, Western Australian, in May 2010, to assess the effects of wheat straw (WS) and chicken manure (CM) biochars and compost with and without phosphorus (P) addition on soil properties and crop production over five growing seasons. The five seasons alternated between winter and summer crops.~The CM and WS biochar and compost treatments significantly increased crop yields and P uptake in 3, 2 and 1 of the five seasons, respectively. The yield increases $(P<0.05)$ were no more than 8\%. By the end of the third season, no differences in crop yields were found that could be attributed to the organic amendments. The addition of P increased crop yields in each winter cropping season. Phosphorus addition explained more than 30\% of the variation in crop yields. Despite marginal P levels and summer drought conditions, arbuscular mycorrhizal root colonisation was not affected by the organic amendments. There were no significant interactions between the organic amendments and P addition in terms of crop yields, P uptake or P uptake efficiency. We conclude that much of the effect of the organic amendments was due to direct nutrient addition which dissipated over time.     

菜心对邻苯二甲酸酯(PAEs)吸收途径的初步研究

采用玻璃室处理和污染土壤覆盖原土壤来控制PAEs来源进行盆栽试验，应用GC/MS联机检测技术初步研究了菜心对PAEs的吸收途径。结果表明：污染土壤处理与污染土壤上覆盖原土壤处理相比，前者菜心茎叶中DBP和DEHP的含量均高于后者，但相差不大，表明菜心茎叶可以吸收污染土壤中挥发出来的DBP和DEHP，而根系吸收运移是菜心茎叶中DBP和DEHP的主要来源途径。玻璃室处理增加了菜心茎叶和根系中DBP的含量，而对DEHP的影响趋势不明显。DBP与DEHP相比，前者更易被菜心根系吸收并向地上部(茎叶)运移，后者主要滞留在根部。     

Effect of liming and applied phosphorus on growth and P uptake of maize (Zea mays subsp.) plant grown in acid soils of West Wollega,Ethiopia

The study aimed at investigating effects of lime and mineral P fertilizers on P uptake and growth of maize (Zea mays subsp.) in greenhouse pot experiment. Combination of four P rates and four lime rates were applied to four highly acidic soil samples and the soils were incubated for a total of 112?days after which maize (Zea mays subsp.) was planted. The maize plants were harvested for analysis after 42?days of growth. Control experiments were also included. Available P (Bray I) and pH were significantly increased after incubation. Maximum available P was obtained in the soils that received the highest P rate and optimum lime rate. Levels of exchangeable acidity were significantly decreased in the P and lime treated soils compared to the control experiments. Plant biomasses and P uptake of maize increased significantly (p?≤?0.001) with application of P and lime compared to the control experiments.     

Phosphorus efficiency of ornamental plants in peat substrates

A pot experiment was conducted to investigate factors contributing to phosphorous (P) efficiency of ornamental plants. Marigold (Tagetes patula) and poinsettia (Euphorbia pulcherima) were cultivated in a peat substrate (black peat 80% + mineral component 20% on a volume basis), treated with P rates of 0, 10, 35, 100, and 170 mg (L substrate)^–1. During the cultivation period, plants were fertigated with a complete nutrient solution (including 18 mg P L^–1) every 2 d. Both poinsettia and marigold attained their optimum yield at the rate of 35 mg P (L substrate)^–1 and the critical level of P in shoot dry matter of both crops was 5–6 mg g^–1. After planting, plant‐available P increased at lower P rates to a higher level for poinsettia than for marigold, but no significant change was observed at higher P rates. Balance sheet calculations indicated that at lower P rates more P was fertigated than was taken up by the plants. Root‐length density, root‐to‐shoot ratio, and root‐hair length of marigold were doubled compared to that of poinsettia. Root‐length density increased with crop growth, and 10 d after planting the mean half distance between roots exceeded the P‐depletion zone around roots by a factor of 3 and 1.5 for poinsettia and marigold, respectively. Thus, at this early stage poinsettia exploited only 10% of the substrate volume whereas marigold utilized 43%. Later in the cultivation period, the depletion zones around roots overlapped for both crops. Taking into account P uptake via root hairs, the simulation revealed that this was more important for marigold compared to poinsettia especially at low P‐supply levels. However, increase of P uptake due to root hairs was only 10%–20% at optimum P supply. For the two lower P levels, the P‐depletion profile around roots calculated for 10 d after planting showed that after 2 d of depletion the concentration at the root surface was below the assumed K_m value (5 μM) and the concentration gradient was insufficient to fit the demand. A higher content of plant‐available P in the substrate was observed for poinsettia compared to marigold in the treatment with P application adequate for optimum growth, because more fertigated P was accumulated during early stages of cultivation due to lower root‐length density of poinsettia. The observed difference of root morphological parameters did not contribute significantly to P‐uptake efficiency, since P mobility in the peat substrate was high.     

红三叶草丛枝菌根对有机磷的吸收

The capacities of two arbuscular mycorrhizal (AM) fungi, Glomus mosseae and Glomus versderme, tomineralize added organic P were studied in a sterilized calcareous soil. Mycorrhizal (inoculated with either of the AM fungi) and non-mycorrhizal red clover (Trghlium pmtense L.) plants were grown for eight weeksin pots with upper root, central hyphal and lower soil compartments. The hyphal and soil compartmentsreceived either organic P (as Na-phytate) or inorganic P (as KH₂PO₄) at tbe rate of 50 mg P kg^-1. No P wasadded to the root compartments. Control pots received no added P. Yields were higher in mycorrhizal than innon-mycorrhizal clover. Mycorrhizal inoculation doubled shoot P concentration and more than doubled total P uptake of plaflts in P-amended soil, irrespective of the form of applied P. The mycorrhizal contributionto inorganic P uptake was 80% or 76% in plants inoculated with G. mosseae or G. verefforme, respectively. Corresponding values were 74% and 82% when Na-phytate was applied. In the root compartments of the mycorrhizal treatments, the proportion of root length infected, hyphal length density and phosphatase activity were all higher when organic P was applied than when inorganic P was added.     

不同供磷状况下CO2浓度升高对番茄根系生长及养分吸收的影响

采用培养试验研究了磷缺乏与正常供磷条件下,CO₂浓度由350μL/L升高至800μL/L苗期番茄的生物量、根系特征和不同器官N、P、K养分含量的变化。结果表明,无论缺磷与否,CO₂浓度升高均能显著增加番茄地上部及根系的干物质积累量,提高根冠比。在磷缺乏条件下,CO₂浓度升高对番茄根系生长的促进主要表现为增加根系的体积和表面积;而在磷正常供应条件下主要表现为同时增加根体积和分根数,有利于形成强壮的根系。在两种供磷水平下,CO₂浓度升高对番茄各器官的N、P、K含量产生不同的稀释效应,但N、P、K总积累量却随CO₂浓度升高而显著增加;而且CO₂浓度与供P水平对番茄植株的N、P、K积累量具有极显著的正交互效应。     

Variation in Yield,Phosphorus Uptake,and Physiological Efficiency of Wheat Genotypes at Adequate and Stress Phosphorus Levels in Soil

Genetic differences among crop genotypes can be exploited for identification of genotypes more suited to a low‐input agricultural system. Twenty wheat (Triticum aestivum L.) genotypes were evaluated for their differential yield response, phosphorus (P) uptake in grain and straw, and P‐use efficiency at the zero‐P control and 52 kg P ha^?1 rates. Substantial and significant differences were obvious among genotypes for both grain and straw yields at stress (8 mg P kg^?1 soil, native soil P, no P addition) and adequate (52 kg P ha^?1) P levels. Genotype 5039 produced maximum grain yield at both P levels. Relative reduction in grain yield due to P‐deficiency stress [i.e., P stress factor (PSF)] ranged between none and 32.4%, indicating differential P requirement of these genotypes. Pasban 90, Pitic 62, Rohtas 90, Punjab 85, and line 4943 did not respond to P application and exhibited high relative yield compared to those at adequate P level. FSD 83 exhibited the best response to P with maximum value for PSF (32.4%). Genotypes were distributed into nine groups on the basis of relationship between grain yield and total P uptake. Rohtas 90 and lines 4072 and 5039 exhibited high grain yield and medium P uptake (HGY‐MP). However, line 5039 with high total index score utilized less P (12.2 kg P ha^?1) than line 4072 and Rohtas 90 (13.5 and 13.6 kg P ha^?1, respectively). Moreover, this genotype also had greater P harvest index (PHI, %) and P physiological efficiency index (PPEI) at stress P level. Pasban 90, Pitic 62, and Pak 81 had the greatest total index score (21), mainly due to high total P uptake, but yielded less grain than lines 5039 and 4072 under low available P conditions. Line 6142 had minimum total index score (15) and also produced minimum grain yield. A wide range of significant differences in PPEI (211 to 365 kg grain kg^?1 P absorbed at stress and 206 to 325 kg grain kg^?1 P absorbed by aboveground plant material at adequate P) indicated differential utilization of absorbed P by these genotypes for grain production at both P levels. It is concluded from the results that wheat genotypes differed considerably in terms of their P requirements for growth and response to P application. The findings suggest that PSF, PHI, and PPEI parameters could be useful to determine P‐deficiency stress tolerance in wheat.     

Phosphorous accumulation and distribution of two ecotypes of Pilea sinofasciata grown in phosphorous-enriched soils

Water eutrophication and poultry manure require an efficacious remediation technology to decrease phosphorous (P) concentration. In this study, the P phytoremediation potential of a mining (ME) and nonmining ecotype (NME) of Pilea sinofasciata were analyzed by pot experiments employing different concentrations of P treatments (CK, P100, P200, P400, P800 and P1600) in tested soil. Growth, P accumulation and P-relevant enzyme activities of P. sinofasciata in ME and NME were assessed after 10 weeks’ plantation. The results showed that there was no significant difference between the shoot biomass of the ME and NME. Higher P concentration and accumulation were observed in the ME than NME for all treatments. Stem P concentration of the ME were 117%, 189%, 152%, 153%, 140% and 139% higher than the NME, and leaf P concentration were 122%, 146%, 138%, 144%, 137% and 113%. Maximum P uptake and phytoextraction was observed at P400 for the ME of P. sinofasciata. Elevated activities of acid phosphatase and phytase in roots were examined in P-enriched soil, and the ME presented higher activities compared with the NME. The results in this paper provide a theoretical basis for the use of ME of P. sinofasciata for phosphorus accumulators and suggest that ME of P. sinofasciata is a promising species for the excess P phytoextraction of P-enriched soils.     

Vesicular-arbuscular mycorrhizal status of various crops in different agricultural soils of northern Greece

Summary This paper reports the results of a survey of the occurrence of vesicular-arbuscular mycorrhizas and spores of the associated fungi in a variety of soils in Northern Greece. The roots of several crop species were examined for the presence of mycorrhizal structures and all samples showed evidence of infection, though to varying degrees. Cotton plants, in particular, had heavy arbuscular infection. Intact and presumably viable spores were relatively infrequent and empty non-viable spores were much more common. Soil characteristics at each sample site are reported also, but only broad generalizations are made at this stage with respect to correlations between mycorrhizal occurrence and edaphic factors.     

Phosphorus availability,root exudates,and microbial activity in the rhizosphere

Field and pot experiments showed that the P demand of wheat is highest in early stages of growth (up to 1.67 μg P per cm² root surface and day). The needed orthophosphate ions H₂PO₄? and HPO₄²-move from soil to the root by diffusion. This process is controlled by the concentration gradient of the diffusible phosphate and the effective diffusion coefficient according to Pick's first law. Root excretions (rhizodeposition) are able to affect both characteristics. The water soluble portion of rhizodeposition contains more than 50% of up to 8 different sugars, 10–40% carboxylic acids and 10–15 amino acids and amides. The composition varies in dependence on the age of the root parts and on nutrition (Zea mays L., Brassica napus L., Pisum sativum L.). Diffusion experiments using small soil blocks showed that 50–75% of the root exudates were decomposed by respiration within 3 days. The rest was largely chemically converted. Originally present sugars disappeared. Due to the biosynthesis of different organic acids from the individual sugars the mobilisation of Ca₃(PO₄)₂ by Pantoea agglomerans increased when the sugar mixture was derived from the rhizodeposition of P deficient plants with more pentoses instead of glucose and fructose (mainly effect of anions). In the rhizosphere therefore a mixture of rhizodeposition and its conversion products exists which affects the binding of phosphorus in soil and the P transport to the root. This should be considered both for the development of new soil extractants and for modelling the P supply to plants.     

Simultaneous Uptake of Multiple Amino Acids by Wheat

ABSTRACT

Recent research has proven that higher plants can utilize amino acids as nitrogen (N) and carbon (C) sources. Most studies have focused on single amino acids with or without inorganic N, but a range of amino acids may be expected under conditions where the main N input derives from turnover of organic N sources. This study investigated the uptake of multiple amino acids by plant roots and further the active versus passive uptake was determined. Under minimum microbial activity conditions, seedlings of wheat (Triticum aestivum L. cv. ‘Baldus’) were exposed to a series of different concentrations of seven mixed amino acids solutions. Samples of the depleted solutions were periodically collected over a period of ten hours to measure the concentration of amino acids. For all tested amino acids passive uptake was a minor contribution compared to the total uptake. The uptake rates of the amino acids were well described by single Michaelis-Menten kinetic equations with R² ranging from 0.87 to 0.96. All of the tested amino acids showed a similar uptake pattern. Wheat plants had the highest affinity (lowest K _m values) for glutamine followed by tryptophan, alanine, arginine, glycine, and serine. The V _max values for amino acids uptake by wheat ranged from 2.26 for tryptophan to 16.6 μmol g^?1 root FW h^?1 in case of serine.     

Phosphorus Uptake and Translocation in Field-Grown Maize after Application of Rare Earth-Containing Fertilizer

ABSTRACT

In recent decades, rare earths and rare-earth containing fertilizers along with nitrogen (N) and phosphorus (P) fertilizers have been used by agriculturists to improve crop yield and quality. The interaction between rare earths and N has been explained in soils, yet little is known of the effect of rare earths on P nutrition of field-grown crops. When maize plants entered the early stem-elongation stage, a rare earth-containing fertilizer (lanthanum (La) and cerium (Ce) as major rare earths) was applied to the soil with irrigation water. Ten days after application of rare earths, there was no significant dose-dependent accumulation of P in the roots and shoots, with one exception where P concentrations increased in the roots at a dosage of 100 kg rare earths ha^{? 1}. However, at maturity, the application of rare earths at less than 10 kg ha^{? 1} significantly improved P uptake by field-grown maize and P transport to the grain. The application of rare earths at less than 10 kg ha^{? 1} increased crop yield. Other studies have shown that at rates less than 10 kg rare earths ha^{? 1}, there was no accumulation of heavy metals and rare earths in the grain, and that the present mean application rate of rare earths (< 0.23 kg ha^{? 1} year^{? 1}) currently applied in China was unlikely to affect the safety of grains in arable soils. Together, these field studies can give a scientific understanding of benefits and risks when utilizing rare earths in agriculture, and of the interaction between rare earths and P nutrition in field-grown crops.     

不同供磷状况下CO2浓度升高对番茄根系生长及养分吸收的影响

采用培养试验研究了磷缺乏与正常供磷条件下,CO2浓度由350μL/L升高至800μL/L苗期番茄的生物量、根系特征和不同器官N、P、K养分含量的变化。结果表明,无论缺磷与否,CO2浓度升高均能显著增加番茄地上部及根系的干物质积累量,提高根冠比。在磷缺乏条件下,CO2浓度升高对番茄根系生长的促进主要表现为增加根系的体积和表面积;而在磷正常供应条件下主要表现为同时增加根体积和分根数,有利于形成强壮的根系。在两种供磷水平下,CO2浓度升高对番茄各器官的N、P、K含量产生不同的稀释效应,但N、P、K总积累量却随CO2浓度升高而显著增加;而且CO2浓度与供P水平对番茄植株的N、P、K积累量具有极显著的正交互效应。     

Effect Of The Sequential Application Of Liquid Organic Manure And Phosphorus On Maize Agronomic Traits And P Uptake In Some Tropical Soils

Improving phosphorus (P) fertility is challenging in tropical soils because of the fixation of P by soil components. The trial investigated the effect of the sequential application of poultry manure extract (tea) and P fertilizer on maize agronomy and P uptake, through screen house and field experiments. Results indicated that poultry manure tea is rich in nutrients but low in P and could be applied with phosphorus fertilizer to enhance P availability and reduce fixation. Application of P before manure tea produced taller maize plant on the field and the highest P uptake in the screen house and on the field. Manure tea also increased maize dry matter and grain yield. Combined application of poultry manure tea and P fertilizer had positive complementary and synergistic effects. It was concluded that manure tea enhances P availability and reduces P fixation by soil aluminum (Al) and iron (Fe) oxides irrespective of the sequence of application.     

不同农业土壤中有效磷的变化与磷平衡的关系

Maintaining soil phosphorus(P) at adequate levels for plant growth requires assessing how the long-term P balance(viz., the difference between P inputs and outputs) results in changes in soil test P. The hypothesis that routinely measured soil properties can help predict the conversion factor of P balance into Olsen P was tested at 39 sites in agricultural areas of the Mediterranean region in Spain. A set of soil samples from each site was analyzed for Olsen P, inorganic P(P extracted using 0.5 mol L~(-1) H_2SO_4), pseudototal P(P extracted using 0.5 mol L~(-1) H_2SO_4 following ignition at 550℃), and organic P(the difference between pseudototal P and inorganic P). Organic and Olsen P were uncorrelated in most of the 39 soil sets, which suggests that organic P content changed little with P inputs and outputs. The slopes of the regression lines of Olsen P against pseudototal and inorganic P, which were used as two different measures of the conversion factor, ranged widely(from 0.03 to 0.25 approximately), with their average values(about 0.10) being similar to those found in long-term experiments conducted in temperate areas. Neither conversion factor was significantly correlated with any routinely measured soil property; however, the conversion factor for inorganic P was significantly lower for calcareous soils than for noncalcareous soils. Our negative results suggest the need to isolate the influence of soil properties from that of management systems and environmental factors relating to P dynamics in future studies.