Forms of phosphorus in bacteria and fungi isolated from two Australian soils

To understand the origin of organic and condensed forms of phosphorus (P) in soils, detailed information about P forms in microorganisms is required. We isolated 7 bacteria and 8 fungi from two Australian soils and analyzed the P forms in their pure cultures by extraction with NaOH-EDTA followed by ³¹P solution nuclear magnetic (NMR) spectroscopy. The bacteria belonged to the actinobacteria and the fungi to the ascomycota, as determined by rDNA sequencing. The proportions of broad forms of P were significantly different between the bacterial and fungal isolates (analysis of similarities, p = 0.001). Ortho-, pyro- and polyphosphate were present in higher proportions in fungi, while monoester and diester P were present in higher proportions in bacteria. Spectral deconvolution of the monoester region revealed 15 distinct resonances. The three major ones, which were identified by spiking experiments as glycerol 1-phosphate, glycerol 2-phosphate and adenosine-5′-monophosphate (AMP), comprised 56–74% of P in the monoester region. Ordination by principal component analysis and testing for treatment effects using analysis of similarities showed significant separation of P distribution in the monoester region between bacterial and fungal isolates (p = 0.007). However, neither group of microorganisms had a specific single P form which might be considered characteristic. As such, it may be difficult to distinguish soil P from bacterial or fungal origins, with the possible exception of a predominantly fungal origin of pyro- and polyphosphate. The identification of three major resonances in the monoester region of microorganisms is important, since the same resonances are found in ³¹P NMR spectra of soil extracts.     

Manganese status op Rajasthan soils

Because of its significant role in the enzymatic and physiological activities of the plant body, manganese has received the attention of several workers. Though manganese may be present in appreciably large quantities, its availability to plants is controlled by various soil properties, the dominant ones among them being pH, calcium carbonate, clay and organic matter present in the soil (BISWAS, (1), (2), LEEPER, (6)).     

长期轮作与施肥对农田土壤磷素形态和吸持特性的影响

通过对黄土旱塬地区长期定位施肥(26a)条件下的不同轮作系统的土壤磷素形态和吸持参数的测定,研究了轮作和施肥对土壤磷素吸持特性和磷素形态的影响,以及土壤磷素吸持参数与磷素形态之间的关系。结果表明,长期轮作与施肥都可以减低土壤磷素的最大吸附量（Qm）,相对于其它轮作和连作,在氮磷（NP）施肥下,小麦-玉米-豌豆轮作可以减低土壤的Qm,在氮磷有机肥（NPM）施肥下,小麦-玉米轮作可以减低土壤的Qm。在施肥相同的条件下,小麦-玉米轮作和小麦-豌豆轮作可以显著增加土壤中各形态无机磷的含量,长期轮作比连作可以增加土壤中的有效磷养分,尤其对Ca2-P的提高效果更为显著。相关分析表明,Qm和磷吸持指数（PSI）与全磷（T-P）、Olsen-P、CaCl2-P、Ca2-P、Ca8-P、Fe-P、Ca10-P和有机磷呈极显著负相关（p＜0.01）,与闭蓄态磷（O-P）呈显著负相关（p＜0.05）,与Al-P关系不显著。土壤有机质（SOM）与Qm、PSI和磷最大缓冲能力（MBC）之间存在极显著负相关关系,与磷吸持饱和度（DPSS）存在显著正相关。通径系数和逐步回归分析表明,在石灰性黑垆土土壤的无机磷形态中,Ca2-P对Olsen-P的贡献最大。     

Evaluation of phosphorus availability from three phosphorus sources in Nigerian soils

Abstract

In an attempt to search for a cheaper source of phosphorus (P), both for direct application and industrial use, three P fertilizers were evaluated in incubation and greenhouse studies. Indigenous Sokoto rock phosphate (SRP) imported, Togo rock phosphate (TRP), and conventional single superphosphate (SSP) were applied on three soil types namely Oxisol, Ultisol, and Alfisol at rates ranging from 0–800 mg P kg^‐1 soil. Evaluation of the P sources was conducted for 12 weeks in incubation study, and five weeks in the greenhouse using maize as test crop. Evaluation of direct application of SSP and SRP on an oxic paleudult was carried out in the field for three years. The results of incubation studies revealed in general, that P availability increased as fertilizer rates increased. The P availability was, however, greater when SSP was applied on the Alfisol than on the Oxisol and Ultisol. The rock phosphates on the other hand were more efficient on acid soils than on soils neutral in pH. Optimum P availability from the fertilizers was observed to occur predominantly between four and eight weeks of incubation. In the greenhouse study, SSP gave the highest cumulative P uptake and optimum rate of application was 200 mg P kg^‐1 soil, while optimum rate for rock phosphate was 400 mg P kg^‐1 soil. The agronomic effectiveness (EA) of the rock phosphates was about 40% relative to SSP on the Alfisol. The EA, however, for TRP and SRP was 120% and 160%, respectively, on the Oxisol, while on the Ultisol, SRP was equally effective as SSP and TRP had 65% effectiveness. The results of the field trial indicated that the SRP had 54%, 83%, and 107% agronomic effectiveness of SSP, respectively, in the first, second, and third year of cropping. Optimum rate for SSP and SRP application was considered to be 50 and 75 kg P₂O₅ ha^‐1, respectively.     

Study of Pedo Genesis of soils of Bundi district (Rajasthan)

The soils of Rajasthan vary from desert sand to heavy clay with all intermediate stages like sandy loam, loam and clay loam. The last two textural groups are more prevalant on the eastern, northeastern and southeastern part of the Aravallies which run almost in the middle of the State from southeast to northeast. The latter groups of soils have more potential from the point of agricultural development in the State. These soils are widely termed as alluvial soils which is a general term to indicate the nontaxonomic group of soils that have parent material of alluvial origin, Riecken ¹⁷, Kelloog ⁸ has referred to the soils developed from the alluvium as classified under variety of world soil groups. In our country many of the alluvial soils have not yet been studied from the soil genesis point of view as a measure to classify the soils under taxonomic groups. Ray Chawdhary and Mukerji ¹⁵, concluded that the alluvial soil groups of our country are ill defined and the classification of these soils need more study. Basu ³ was able to distinguish the genetic characteristics of the soils of Deccan. Agrawal and Mukherji ¹⁾ established that distinct genetic soil types have come into expression under Gangetic alluvium. Agarwal ² has concluded about the three types of soils in the toposequence with precipitated calcium throughout, zone of calcium accumulation in the bottom of the profile and soils free of calcium, and suggests that the soils of Gangetic plain be called as derived from alluvium. A process of reclassifying the alluvial soil series into taxonomic groups is a common trend in the United States since the last two decades.     

Quantifying microbial biomass phosphorus in acid soils

 This study aimed to validate the fumigation-extraction method for measuring microbial biomass P in acid soils. Extractions with the Olsen (0.5 M NaHCO₃, pH 8.5) and Bray-1 (0.03 M NH₄F–0.025 M HCl) extractants at two soil:solution ratios (1 : 20 and 1 : 4, w/v) were compared using eight acid soils (pH 3.6–5.9). The data indicated that the flushes (increases following CHCl₃-fumigation) of total P (P_t) and inorganic P (P_i) determined by Olsen extraction provided little useful information for estimating the amount of microbial biomass P in the soils. Using the Bray-1 extractant at a soil:solution ratio of 1 : 4, and analysing P_i instead of P_t, improves the reproducibility (statistical significance and CV) of the P flush in these soils. In all the approaches studied, the P_i flush determined using the Bray-1 extractant at 1 : 4 provided the best estimate of soil microbial biomass P. Furthermore, the recovery of cultured bacterial and fungal biomass P added to the soils and extracted using the Bray-1 extractant at 1 : 4 was relatively constant (24.1–36.7% and 15.7–25.7%, respectively) with only one exception, and showed no relationship with soil pH, indicating that it behaved differently from added P_i (recovery decreased from 86% at pH 4.6 to 13% at pH 3.6). Thus, correcting for the incomplete recovery of biomass P using added P_i is inappropriate for acid soils. Although microbial biomass P in soil is generally estimated using the P_i flush and a conversion factor (k _P) of 0.4, more reliable estimates require that k _P values are best determined independently for each soil. Received: 3 February 2000     

Kinetics of microbial phosphorus uptake in cultivated soils

Knowledge about the role of microorganisms in P cycling at conditions of constant soil respiration rates and constant size of microbially bound P is lacking. To study the kinetics of microbial P uptake and cycling under such conditions, soils differing in biological activity were ³³PO₄ labelled by introducing a carrier-free tracer solution and incubated for 56 days. The ³³PO₄ incorporation into the fraction of microbial P releasable by chloroform treatment (P_chl) was assessed and the isotopic composition [=specific activity (SA); SA=³³PO₄/³¹PO₄] of P_chl and soil solution P compared. Soils were taken from a 20-year-old field experiment including a non-fertilised control (NON), a minerally fertilised conventional (MIN) and two organic farming systems [bio-organic (ORG); bio-dynamic (DYN)]. Tracer P incorporation continuously increased during incubation in DYN, ORG and MIN soils. It decreased in the order DYN>ORG>MIN, with differences in ³³PO₄ uptake between the farming systems being higher than suggested by the differences in the amount of P_chl. In the P-deficient NON soil, the highest initial incorporation of tracer P was found, but no additional uptake could be detected thereafter. In all soils, the SA of P_chl converged to the SA of the soil solution with increasing time. Since P_chl remained almost constant during the experiment, the findings suggest an intensive uptake of P from the soil solution into P_chl and concomitant release of P back to the soil solution and, thus, a rapid cycling through P_chl. Intensive P cycling between P_chl and the soil solution was confirmed in an additional experiment where microbial activity was stimulated by glucose and N additions.     

Forms of aluminium in some acid permanent grassland soils

The more labile forms of aluminium in a range of soils from areas of permanent grassland were determined with a number of selective extractants. The amounts of exchangeable A1 extracted with molar KCl were dependent upon pH, while the amounts exchangeable with 0.3 M LaCl³, although much greater, were not well correlated with pH. There were good correlations between soil organic C content and A1 extracted by (i) 0.5 M EDTA and (ii) 0.1 M potassium pyrophosphate. Pyrophosphate extracted greater amounts than any of the other extractants (sodium citrate/dithionite, ammonium oxalate (dark), acid oxalate (UV radiation), as well as those already mentioned). It was concluded that much of the extractable A1 in soils was associated with organic matter. Addition of lime to one of the soils reduced the amount of A1 extracted by all reagents except dithionite and acid oxalate solutions. There were considerable differences between soils in their release of A1 to continuous leaching with 0.01 M CaCl². Despite these differences between the soils in organically bound extractable Al, the differences in the amounts and patterns of release of A1 with CaCl² did not appear to be related to organic matter contents, nor to the other determined properties.     

Aspects of phosphorus transfer from soils in Europe

Imbalanced nutrient management has caused soil phosphorus (P) to become an environmental rather an agronomic problem in more economically developed countries. This subject has been the topic of numerous journal special issues, conferences, and reviews but we consider yet another review of this subject is necessary with the main target of providing a point of view on nonpoint transfer from soils and control strategies for an improved environmental management of P. This review considers the causes of the excessive P transfer from soil to surface water in Europe and the scientific knowledge necessary to develop control strategies.     

Variation of four phosphorus properties in woodland soils

Variation in total, organic and available-P contents and phosphatase activity of P-deficient soils of some English Lake District woodlands of differing vegetative composition were examined in relation to individual woodlands, two depths in the soil profile, mull, moder and mor humus types, and different times of the year. Depth in the soil profile was a more important source of variation in the P properties than different woodlands. Soils in individual woodlands differed in their degree of variability in the four P properties. Available P contents and phosphatase activities were more variable than total and organic P contents. Available-P and organic-P contents and phosphatase activity showed seasonal variation. Seasonal variation in available-P was almost as great as differences in available-P between woodlands. Total and organic-P contents showed similar patterns of variation with respect to individual woodlands, humus type and soil depth. Differences in degree of variation within woodlands and differences in degree and pattern of variation of the four P-properties may need to be taken into account in soil sampling programmes of studies comparing soils under differing vegetation regimes.Different interpretations of the variation in the soil-P properties were obtained by expressing the data respectively in terms of soil weight (g^?1 soil) or soil volume (cm^?1 soil), due to marked variation in bulk-densities of the woodland soils. It is suggested that where soils vary in bulk-density, soil data should be expressed in terms of soil volume.The P-deficiency of the woodland soils is probably associated with the relatively low total P content per unit volume of soil and the high proportion of it which is organically bound.     

Forms of application of phosphorus fertilization on carrot

Abstract

The aim of this study was to evaluate the effect of forms of application and application rates of phosphate fertilizer on the agronomic characteristics of carrot. A field experiment was carried out in a 2?×?7 factorial arrangement, consisting of two forms of application of the P source (over the whole area or specifically in the strip of the double row), and seven application rates of P₂O₅ in randomized blocks, with four replicates. Shoot dry matter yield increased linearly, and root dry matter yield increased exponentially with an increase in P rate. Total and commercial yields increased exponentially, with higher yield for P application over the whole area. However, the agronomic efficiency for site-specific application was higher. Higher rates of P application caused increases in the soil attributes and technical traits of carrot, and the application of phosphorus fertilizer is more efficient when performed in a site-specific manner.     

Microbial biomass phosphorus and its significance in predicting phosphorus availability in red soils

Abstract

Red soils are widespread in Southern China and other subtropical regions in the world. An improved management of phosphorus (P) is crucial for sustainable agriculture and environmental quality in red soil regions. Plant‐availability of P in red soils mainly depends on fertilization and biological cycling. Both laboratory analyses and greenhouse experiments were conducted to examine the relationships between plant P uptake, chemical index of P, and microbial biomass P in red soils with different fertility levels. Microbial biomass P ranged from 2.1 to 43 mg kg^‐1 in the red soils and was significantly correlated with total P (r=0.84*), organic P (r=0.87*), or Bray I extractable P (r=0.94**). Extractable P plus organic carbon accounted for >85% of the variation in microbial biomass P in the red soils. The significant relationship between microbial biomass P and extractable P suggests that microbial biomass P has a great potential in predicting P‐supply ability in soil. Greenhouse experiments showed that there were close relationships between ryegrass dry matter yield, plant P uptake or tissue P concentration and microbial biomass P in the red soils. The corresponding correlation coefficients were 0.79*, 0.90*, and 0.91*, respectively. These results imply that microbial biomass P plays an important role in the availability of P to plants, and is a potential biological index of P availability in the red soils.     

Microbial biomass phosphorus contributions to phosphorus solubility in riparian vegetated buffer strip soils

This study tests the hypothesis that microbial biomass phosphorus (P) makes a significant contribution to P solubility in riparian buffer strip soils. In 36 soils collected from buffer strips within three UK soil associations, water-extractable inorganic P solubility was most strongly related to NaHCO₃ extractable inorganic P. However, within individual soil associations where soil pedological properties and management were similar, water-extractable inorganic P was most strongly related to microbial biomass P. These results highlight the difficulty in predicting dissolved P leaching risk based on agronomic soil P tests alone and the dissolved P leaching risk presented by having soils high in organic matter and microbial biomass P in close proximity to surface waters.     

不同磷水平土壤上磷启动肥对玉米生长效应的研究

探究不同磷水平土壤施用磷启动肥对玉米生长和磷吸收的影响,为玉米生产中合理施用磷启动肥提供理论依据。通过两因素盆栽试验,人工设置3个土壤磷肥力水平,分别为低磷土壤(LP,P 2.47     

EDTA extractable phosphorus in soils as related to available and inorganic phosphorus forms

Abstract

Twenty surface soil samples, representing two major soil orders alfisols and vertisols were extracted with 0.01N Na₂ EDTA solution (pH 4.8) at a soil/solution ratio of 1:25. Phosphorus in the extract was determined following ammonium molybdate‐stannous chloride colorimetric method. The EDTA extractable P showed significant positive correlations with extractable P according to the Olsen, Morgan, Bray 1 and 2 and also with inorganic phosphorus fractions associated with Al, Ca and Fe.     

Forms of heavy metal compounds in soils of the steppe zone

A parallel scheme of extractions was used for determining heavy metal compounds in uncontaminated and artificially contaminated soils of Rostov oblast. A method for calculating the contents of complex and specifically adsorbed metal compounds from the difference in their concentrations in different extracts was suggested. It was found that the portion of firmly fixed metal compounds decreases and the portion of potentially mobile (exchangeable, complex, and specifically adsorbed) metal compounds increases with an increase in the degree of contamination of chernozems and chestnut soils with lead, copper, and zinc over a one-to two-year-long observation period. This was due to the capacity of heavy metal ions to substitute for the exchangeable cations in the exchange complex of the studied soils, to form complexes with the soil organic matter, and to participate in the specific adsorption. Some differences in the behavior of different metals were found.     

澳大利亚东部地区一些酸性硫酸盐土壤磷的特征

Forty-five acid sulfate topsoil samples (depth < 0.5 m) from 15 soil cores were collected from 11 locations along the New South Wales coast, Australia. There was an overall trend for the concentration of the HC1-extractable P to increase along with increasing amounts of organic C and the HCl-extractable trivalent metals in the topsoils of some less-disturbed acid sulfate soils (pH < 4.5). This suggests that inorganic P in these soils probably accumulated via biological cycling and was retained by complexation with trivalent metals or their oxides and hydroxides. While there was no clear correlation between pH and the water-extractable P, the concentration of the water-extractable P tended to increase with increasing amounts of the HCl-extractable P. This disagrees with some established models which suggest that the concentration of solution P in acid soils is independent of total P and decreases with increasing acidity. The high concentration of sulfate present in acid sulfate soils appeared to affect the chemical behavior of Pin these soil systems. Comparison was made between a less disturbed wetland acid sulfate soil and a more intensively disturbed sugarcane acid sulfate soil. The results show that reclamation of wetland acid sulfate soils for sugarcane production caused a significant decrease in the HCl-extractable P in the topsoil layer as a result of the reduced bio-cycling of phosphorus following sugarcane farming. Simulation experiment shows that addition of hydrated lime had no effects on the immobilization of retained P in an acid sulfate soil sample within a pH range 3.54.6. When the pH was raised to above 4.6, soluble P in the soil extracts had a tendency to increase with increasing pH until the 15th extraction (pH 5.13). This, in combination with the poor pH-soluble P relationship observed from the less-disturbed acid sulfate soils, suggests that soluble P was not clearly pH-dependent in acid sulfate soils with pH < 4.5.     

Organic matter effects on phosphorus sorption in sandy soils

Phosphorus (P) sorption processes in soils contribute to important problems in agriculture: a deficiency of this plant nutrient and eutrophication in aquatic systems. Soil organic matter (SOM) plays a major role in sorption processes, but its influence on P sorption remains unclear and needs to be elucidated to improve the ability to effectively manage soil P. The aim of this research was to investigate the influence of SOM on P sorption. The study was conducted in sandy soil profiles and in topsoils before and after removal of SOM with H₂O₂. The results were interpreted with the Langmuir and Freundlich isotherms. Our results indicated that SOM affected P sorption in sandy soils, but that P sorption also depended on specific soil properties (e.g. values of the degree of P saturation (DPS), P sorption capacity (PSC) and pH) often related to land use. Removal of SOM decreased PSC in most of the topsoils tested; other soil properties became important in controlling P sorption. An increase in P desorption observed after SOM removal indicated that SOM was potentially that soil constituent which increased P binding and limited P leaching from these sandy soils.     

Modelling long-term phosphorus leaching and changes in phosphorus fertility in excessively fertilized acid sandy soils

The sound management of agricultural soils that are heavily loaded with phosphorus (P) involves minimizing the losses of P responsible for eutrophication of surface waters, while ensuring enough P for crops. This paper describes a simple model to examine the compatibility of these two objectives in acid sandy soils in a temperate humid climate. The model is based on several assumptions regarding reversible and irreversible P sorption by P-reactive soil compounds (mainly poorly crystalline Fe and Al oxides) and release of P to water (water-P test). Model inputs are amount of P leached, P uptake by crops, and contents of poorly crystalline Fe and Al oxides in soil. The model predicts that reducing the amount of leached P to what is environmentally acceptable (e.g. 0.44 kg P ha^–1 year^–1, equivalent to 1 kg P₂O₅ ha^–1 year^–1) results in the long run in available soil P test values below target concentrations for optimum crop growth. When the amount of leached P is set to a fixed value the model predicts that soils with large contents of Fe and Al oxides can maintain the initial soil P test values for longer periods than other soils. The content in available P decreases if fertilizer P is applied to the soil at a rate equal to P uptake by crops. These results stress the difficulties involved in trying to make agricultural and environmental needs compatible in acid sandy soils.