植保四轴飞行器的模糊PID控制

针对当前植保四轴飞行器在作业过程中自身载荷发生改变后的飞行控制性能下降、抵抗环境扰动能力差的问题,该文改进了传统比例积分微分(proportion,integration,differentiation,PID)控制算法,提出了一种模糊PID控制算法。该文通过研究四轴飞行器的姿态解算和飞行原理,设计了以STM32系列的单片机为核心处理器的四轴飞行控制系统。采用AHRS模块实时解算飞行器姿态参数,结合模糊控制和PID控制算法调节电机的输出量来控制飞行姿态。试验结果表明:与传统PID相比,模糊自整定PID控制算法适应性强,参数整定简单,系统的动态响应能力和稳定性获得了提高,实现了四轴飞行器的稳定飞行。该文为植保无人机控制算法研究提供了一定的参考。     

农机具姿态倾角测量系统设计与试验

农机具姿态倾角测量技术是实现农机装备精准作业的关键技术之一。为进一步提高农机装备作业质量,以ADIS16445微惯性MEMS传感器和STM32F446核心处理器搭建硬件平台,以欧拉角法解算姿态,建立卡尔曼滤波模型融合加速度计与陀螺仪信息,实现农机具姿态倾角的精准测量。融合算法模型考虑陀螺仪零偏特性,并根据MEMS微传感器运动特性,自适应模型误差协方差矩阵Q与R,适应不同工况下农机具姿态倾角测量。采用SGT320E三轴多功能转台与BD982双天线定位测姿模块对系统进行测试与验证。三轴多功能转台试验结果表明,ADIS16445内置陀螺仪与加速度计性能合格,满足系统设计硬件要求;卡尔曼滤波融合模型精准有效,倾角静态测量误差精度为0.15°,动态测量精度典型值为0.3°,最大测量误差为0.5°。田间作业试验结果表明,自适应模型能保证农机具姿态倾角测量系统在不同工况下的测量精度,更稳定可靠,测量平均误差为0.55°。该文研究的农机具姿态倾角测量系统可满足农机装备精准作业要求。     

农田信息采集用多旋翼无人机姿态稳定控制系统设计与试验

农田信息快速采集是精准农业的基础。为快速、高效、准确、节能获取农田信息,该文搭建了多旋翼无人机平台,设计了以STM32F407为主控制器的多旋翼飞行控制系统。采用了比例积分微分(proportion,integration,differentiation,PID)双闭环控制策略,外环为角度反馈,内环为角速度反馈。通过工程凑试法得到合适的PID控制参数。运用专家控制策略改进上述控制方法,使控制参数适应无人机姿态变化。对所设计的无人机控制系统进行抗干扰和阶跃响应试验。系统在受到30?横滚与俯仰角干扰后,其对应恢复平衡时间均在3.4 s内,航向角30?干扰后恢复时间在4 s内。系统横滚与俯仰角阶跃响应调节时间在1~2 s内,航向角在3.4 s内。试验结果表明:双闭环PID控制策略实现多旋翼无人机姿态稳定控制,专家控制策略增强无人机的抗干扰能力。在室外农田环境中,无人机能根据指令在1~2 s内快速调整姿态。当姿态受风影响发生倾斜时,陀螺仪测量角速度大于3(?)/s,采用的控制策略能迅速调整电机转速,保持无人机姿态稳定平衡。试验证明该控制系统稳定可控且具有较强抗干扰性,满足多旋翼无人机低空采集农田信息的要求。     

基于Labview的无人机飞行状态实时监测评估系统设计

为了实现无人机飞行状态信息的自动化采集和性能评估,该文设计了基于 Labview 的无人机飞行状态实时监测评估系统,该系统利用传感器采集无人机的飞行状态信息：包括三轴姿态角、三轴角速度、三轴速度、三轴加速度、GPS经纬度及海拔高度、环境温度和气压等。无线传输模块将经过简单处理之后的信息传输至PC机,基于Labview建立的监测评估软件对这些数据进一步处理之后,实时图形化显示三轴姿态、飞行高度、二维轨迹、三维轨迹和航迹偏差;根据三轴姿态信息实时模拟无人机姿态,自动计算飞行里程,并自动保存所有数据。飞控手目视操控无人机的试验结果表明：平均航迹偏差高达5.2 m,定高飞行的平均高度偏差为0.9 m,横滚角和俯仰角波动幅度均在8°以内,整个测试过程中传感器温度下降了2℃。数据分析结果与系统输出结果一致,该系统运行稳定,输出结果可靠,能够用于实时监测、图形化显示、评估和记录无人机飞行状态信息,为无人机飞行性能的评估及飞控手的训练提供参考。     

基于BDS和IMU的挖掘机铲斗位姿测量方法与试验

为提高农田建设中挖掘机施工作业精度和智能化程度，本文提出了一种基于BDS（BeiDou Navigation Satellite System，北斗卫星导航系统）和IMU（Inertial Measurement Unit，惯性测量单元）的挖掘机铲斗位姿测量方法。首先，采用IMU测量挖掘机各执行机构的姿态角信息，解算获得挖掘机车体坐标系下铲斗末端的三维坐标，利用双天线BDS和IMU检测车体的位置和姿态建立了挖掘机铲斗末端三维坐标的实时解算模型，并设计了融合双天线BDS和IMU输出高频率高精度位姿的卡尔曼滤波算法。模拟挖掘机实际施工场景进行了静态和动态试验，采用全站仪验证铲斗末端三维坐标解算值。试验结果表明，该方法能准确实时测量挖掘机铲斗末端三维坐标，挖掘机铲斗末端三维坐标解算值与全站仪实测值的运动轨迹变化一致，同一时刻空间两坐标点距离均方根偏差小于30mm，三个轴向坐标动态测量均方根偏差均在20mm内，绝对偏差≤30mm的数据占比不低于95.35%，挖掘机铲斗位姿的准确测量为挖掘机精准施工智能引导提供了基础。     

基于MEMS传感器的两轴姿态调整系统设计与试验

在精准农业生产过程中,传感器实时采集作物信息或环境状态,传感器与作物的相对位置,直接影响到采集数据的准确性,及后期处理的效率,甚至影响到作业的效果。而田间道路、垄间颠簸,会影响传感器与作物相对位置,造成信息失真和不准确,为了减少地面不平整干扰对传感器位置的影响,该文提出了基于MEMS传感器步进电机驱动的两轴姿态调整系统。该研究分析了系统的工作原理和控制方法,以陀螺仪、重力加速度计为姿态测量元件,步进电机为驱动部件,设计基于单片机控制的两轴姿态调整系统平台软硬件结构。系统采用单片机对陀螺仪和加速度计信息的实时采样,建立了多传感信息的融合算法和姿态判定模型,可以实时分析检测对象姿态,并输出控制步进电机,对平台姿态进行补偿调整,保持控制对象的相对惯性空间方位不变,实现了平台姿态平衡的快速控制。同时系统加入了绝对位置传感器,实现初始工作状态的自动复位。测试试验结果表明,系统运行稳定,单轴姿态调整精度在平整坡路状态下最大误差在0.5°以内,在田间颠簸路况运行下最大误差在3.0°以内,能够满足信息采集和检测过程中姿态自动调整、保持相对位置的控制要求。利用该控制系统,能够提高信息采集的准确性,在精准农业生产中具有应用作用。     

田间作业车辆外部加速度辨识与姿态测量系统研制

复杂田间作业环境与精细作业效果要求农机装备具备实时精准感知农机具姿态的能力,田间作业时普遍存在的车辆外部加速度对此带来挑战。为进一步提高农机装备作业质量,该文以6轴微惯性传感器为硬件传感器,以方向余弦矩阵法进行姿态解算,基于一阶外部加速度模型设计卡尔曼滤波融合算法,实现动态情况下田间作业车辆外部加速度辨识与姿态精准估计。分别采用Innova 2100型摇床与装配有MTi300航向姿态参考模块的高地隙喷雾机对系统进行试验验证。摇床试验结果表明:在外部加速度小于10g情况下,系统对外部加速度辨识误差小于0.214 m/s2;田间作业高地隙喷雾机试验结果表明,相比于MTi300,横滚角最大误差为0.23?,俯仰角最大误差为0.39?。说明该文研制姿态测量系统可准确辨识外部加速度与测量姿态,研究结果可为满足精细农业作业要求的姿态测量系统研发提供依据。     

农机具自动调平控制系统设计与试验

为了使农机具在田间作业时保持水平,该文设计了一种农机具自动调平控制系统。采用拖拉机横向倾角卡尔曼滤波算法融合加速度计和陀螺仪2个传感器数据获得拖拉机实时倾斜角度,直线位移传感器测量调平液压油缸伸长量并建立农机具和拖拉机的相对倾斜角度转换函数,通过控制电磁换向阀实现农机具水平控制。在三轴多功能转台上对拖拉机倾角实时测量算法进行了测试,并在田间对农机具自动调平系统进行了试验,结果表明,拖拉机横滚角传感系统能在动态条件下准确地测量拖拉机实时倾角,在转台上测量角度平均绝对误差≤0.15°,均方根误差≤0.18°,在水田激光平地机作业时测量角度平均绝对误差0.40°;自动调平控制系统能较好地实现平地铲调平控制,平地铲倾斜角度平均绝对误差0.52°,均方根误差0.24°,最大误差1.15°,相对于原水田激光平地机水平控制系统控制精度提高了0.5°。该研究为农机具水平自动调平提供了方法,能够提升农机具作业质量。     

基于MEMS传感器的两轴姿态调整系统设计与试验

在精准农业生产过程中,传感器实时采集作物信息或环境状态,传感器与作物的相对位置,直接影响到采集数据的准确性,及后期处理的效率,甚至影响到作业的效果。而田间道路、垄间颠簸,会影响传感器与作物相对位置,造成信息失真和不准确,为了减少地面不平整干扰对传感器位置的影响,该文提出了基于MEMS传感器步进电机驱动的两轴姿态调整系统。本研究分析了系统的工作原理和控制方法,以陀螺仪、重力加速度计为姿态测量元件,步进电机为驱动部件,设计基于单片机控制的两轴姿态调整系统平台软硬件结构。系统采用单片机对陀螺仪和加速度计信息的实时采样,建立了多传感信息的融合算法和姿态判定模型,可以实时分析检测对象姿态,并输出控制步进电机,对平台姿态进行补偿调整,保持控制对象的相对惯性空间方位不变,实现了平台姿态平衡的快速控制。同时系统加入了绝对位置传感器,实现初始工作状态的自动复位。测试试验结果表明,系统运行稳定,单轴姿态调整精度在平整坡路状态下最大误差在0.5°以内,在田间颠簸路况运行下最大误差在3.0°以内,能够满足信息采集和检测过程中姿态自动调整、保持相对位置的控制要求。利用该控制系统,能够提高信息采集的准确性,在精准农业生产中具有应用作用。     

基于GNSS姿态与电机编码器的农机转向角度测量系统研制

典型的农业机械自动驾驶系统需要在车辆转向轮上安装角度传感器测量转向角度,存在安装不便与可靠性差问题。该研究提出一种基于GNSS模块和电机编码器组合的转向轮转向角度测量系统。该系统通过组合利用GNSS姿态测量值与运动模型得到转向轮期望角度,利用电机转向速度和全液压转向阀的传递模型推算转向角度变化值,经卡尔曼滤波融合解算得到车辆转向轮的实时转向角度。与霍尔式绝对角度传感器对比的动态测试结果表明,该系统在直线行驶时的测量标准方差小于0.91°,在转向轮-10°～+10°区间,测量标准方差小于1.0°;直线自动驾驶作业时的导航误差小于2.5 cm,曲线作业自动驾驶的导航误差小于9.0 cm,满足农机自动调头等场景应用。     

低氮和干旱胁迫对富士和秦冠生长及氮素利用的影响

【目的】以富士（Fuji）、 秦冠（Qinguan）嫁接在平邑甜茶（Malus hupehensis Rehd.）上的当年生盆栽苗为试验材料,采用砂培方法,研究了缺氮胁迫和干旱对富士和秦冠生长情况、 光合参数、 植株各部位氮磷钾含量及氮素利用效率的影响,分析比较了低氮干旱条件下富士和秦冠生长及氮素利用的差异,以期为果树生产高效肥水利用提供理论指导。【方法】试验共设四个处理: 正常氮正常水（ZZ）、 低氮正常水（DZ）、 正常氮干旱（ZG）、 低氮干旱（DG）。氮素和水分均设置两个水平,分别为正常氮（6 mmol/L NO-3-N）、 低氮（0.3 mmol/LNO-3-N）、 正常供水（保持盆中砂子相对含水量为饱和含水量的80%~85%）、 干旱处理（保持盆中砂子相对含水量为饱和含水量的60%~65%）。【结果】富士和秦冠的生物量（茎和叶）、 株高茎粗等生长指标以及光合速率、 气孔导度、 蒸腾速率均为正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）,并且相对应处理下秦冠的以上指标均高于富士;正常供水下,缺氮处理使富士、 秦冠的根冠比比正常氮处理均有所增加,富士提高了2.05%,秦冠提高了22.40%。富士和秦冠的氮、 磷、 钾含量均表现出正常氮正常水（ZZ）＞低氮正常水（DZ）＞正常氮干旱（ZG）＞低氮干旱（DG）; 氮、 钾元素含量在植株各部位的分布顺序依次是叶＞根＞茎,磷元素则是根＞叶＞茎;光合氮素利用效率（PNUE）和氮素利用效率表现为秦冠处理之间差异极显著,富士处理之间差异不显著;秦冠的PNUE和NUE明显高于富士,在低氮正常水（DZ）处理下,秦冠氮肥利用率比富士高42.07%,在低氮干旱（DG）处理下高64.14%;低氮胁迫下富士和秦冠的NUE显著提高,并且秦冠提高的幅度高于富士。【结论】施用氮肥能够显著提高富士与秦冠的干物质量,同等水肥条件下,秦冠生长优于富士;水分亏缺会减少叶片对氮的吸收,干旱条件下适度增施氮肥,可提高果树的抗旱能力;低氮干旱胁迫下秦冠的生长指标、 光合指标及氮素利用效率指标均优于富士,表现出较强的抗低氮干旱胁迫的能力。     

Pasture Vegetation Elemental Analysis by Laser-Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy (LIBS) is a new technique for the analysis of plant material. This study investigates the application of LIBS to pasture-based plant samples. The LIBS measurements were obtained from pelletized pasture samples (100 samples) that had been also analyzed by inductively coupled plasma–optical emission spectroscopy (ICP-OES) following microwave digestion for calibration and comparison purposes. Comparisons for elements sodium (Na), potassium (K), magnesium (Mg), calcium (Ca), manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), boron (B), phosphorus (P), and sulfur (S) showed that LIBS could be used for almost all the standard profile total elements with concentrations down to low mg/kg levels (observed error of Na: 0.024 percent, K: 0.18 percent, Mg: 0.016 percent, Ca: 0.073 percent, P: 0.017 percent, Mn: 31 mg/kg, Fe: 150 mg/kg, Zn: 6.6 mg/kg, and B: 1.1 mg/kg). Elemental analysis at less than mg/kg levels was not possible using LIBS. The elements S and Cu were particularly difficult to analyze with reliability using LIBS at the concentration levels found in the plant samples. Replacing microwave digestion and subsequent ICP analysis with a direct analysis of dried plant samples using LIBS has the potential to improve the productivity and reduce the cost of testing.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications from individuals who have expertise and a record of accomplishment in research areas related to ecology,agro-hydrology,     

Macroinvertebrates in North American tallgrass prairie soils: effects of fire, mowing, and fertilization on density and biomass

The responses of tallgrass prairie plant communities and ecosystem processes to fire and grazing are well characterized. However, responses of invertebrate consumer groups, and particularly soil-dwelling organisms, to these disturbances are not well known. At Konza Prairie Biological Station, we sampled soil macroinvertebrates in 1994 and 1999 as part of a long-term experiment designed to examine the effects and interactions of annual fire, mowing, and fertilization (N and P) on prairie soil communities and processes. For nearly all taxa, in both years, responses were characterized by significant treatment interactions, but some general patterns were evident. Introduced European earthworms (Aporrectodea spp. and Octolasion spp.) were most abundant in plots where fire was excluded, and the proportion of the total earthworm community consisting of introduced earthworms was greater in unburned, unmowed, and fertilized plots. Nymphs of two Cicada genera were collected (Cicadetta spp. and Tibicen spp.). Cicadetta nymphs were more abundant in burned plots, but mowing reduced their abundance. Tibicen nymphs were collected almost exclusively from unburned plots. Treatment effects on herbivorous beetle larvae (Scarabaeidae, Elateridae, and Curculionidae) were variable, but nutrient additions (N or P) usually resulted in greater densities, whereas mowing usually resulted in lower densities. Our results suggest that departures from historical disturbance regimes (i.e. frequent fire and grazing) may render soils more susceptible to increased numbers of European earthworms, and that interactions between fire, aboveground biomass removal, and vegetation responses affect the structure and composition of invertebrate communities in tallgrass prairie soils.     

Variation in quality parameters between and within 14 Nordic tree fruit and berry species

Abstract

A 3-year study was carried out to investigate quality parameters in 14 tree fruit and berry species grown in southern Norway. The species were blueberry, apple, aronia, sour cherry, sweet cherry, red raspberry, strawberry, blackcurrant, gooseberry, red currant and elderberry, harvested along with wild bilberry, cloudberry and lingonberry. Significant differences between species were identified for all quality parameters. The coefficient of variation between species was lowest for pH (12.5%), dry matter (18.9%) and soluble solids (25.3%), followed by titratable acids (59.3%), total phenolics (83.8%), antioxidant capacity FRAP (85.7%) and antiradical power by the DPPH-assay (97.8%), total monomeric anthocyanins (132%) and ascorbic acid (137%). Average coefficient of variation within species were lower and ranged from 4 (pH) to 62% (ascorbic acid). Only the FRAP values were significantly affected by harvesting year with lower levels in 2004 than in 2005 and 2006. There were significant interactions between species and harvesting year for dry matter, soluble solids, pH, ascorbic acid and FRAP. The results indicate generic ranges in composition within species independent upon growing location and climate, and the composition of the tree fruits and berries is not likely to deviate from these ranges. It is concluded that desirable composition of tree fruits and berries and their products should primarily be achieved by selection among species rather than searching fors broadened variation within individual species.     

Potassium Fixation and Release Characteristics in Rhizosphere and Nonrhizosphere Soils for a Rapeseed–Rice Cropping Sequence

Potassium (K) fixation and release in soil are important factors in the long-term sustainability of a cropping system. Changes in K concentration and characteristics of K fixation and release in rhizosphere and nonrhizosphere soils in the rapeseed (Brassica napus L.)–rice (Oryza sativa L.) rotation were investigated using a rhizobox system. The concentrations of different forms of K in both rhizosphere and nonrhizosphere soils decreased with plants compared to without plants, regardless of K fertilizer application. Potassium uptake by crops mainly came from the rhizosphere soil. In the treatment without K fertilizer (–K), the main form of K supplied by the soil to the crops was 1.0 mol L^?1 nitric acid (HNO₃) nonextractable K, followed by nonexchangeable K, and then exchangeable K. In the treatment with K fertilizer (+K), the main K forms supplied by the soil to the crops were exchangeable K and nonexchangeable K. The amount and rate of K fixation after one cycle of the rapeseed–rice rotation was greater in rhizosphere soil than in nonrhizosphere soil. The amount and rate of K fixation of soil in the +K treatment were significantly less than in the –K treatment. The cumulative amounts of K released with 1.0 mol L^?1 ammonium acetate (NH₄OAc) and 1.0 mol L^?1 HNO₃ extraction increased with the increasing numbers of extractions, but the K-releasing power of soil by successive extraction decreased gradually and finally became almost constant. The release of K was less in rhizosphere soil than in nonrhizosphere soil. The release of K in the +K treatment was similar to that in the –K treatment in rhizosphere soil, but the K release in nonrhizosphere soil was greater with the +K than the –K treatment. Overall, the information obtained in this study will be helpful in formulating more precise K fertilizer recommendations for certain soils.     

Electron donor and pH relationships for biologically enhanced dissolution of chlorinated solvent DNAPL in groundwater

Biologically enhanced dissolution offers a method to speed removal of chlorinated solvent dense non-aqueous-phase liquid (DNAPL) sources such as tetrachloroethene (PCE) and trichoroethene (TCE) from aquifers. Bioremediation is accomplished by adding an electron donor to the source zone where fermentation to intermediates leading to acetic acid and hydrogen results. The hydrogen and possibly acetic acid are used by dehalogenating bacteria to convert PCE and TCE to ethene and hydrochloric acid. Reductive dehalogenation is thus an acid forming process, and sufficient alkalinity must be present to maintain a near neutral pH. The bicarbonate alkalinity required to maintain pH above 6.5 is a function of the electron donor: 800 mg/L of bicarbonate alkalinity is sufficient to achieve about 1.2 mM TCE dechlorination with glucose, 1.7 mM with lactate, and a much higher 3.3 mM with formate. Laboratory studies indicate that in mixed culture, formate can be used as an electron donor for complete conversion to ethene, contrary to pure cultures studies indicating it cannot. Various strategies can be used to add electron donor to an aquifer for DNAPL dehalogenation while minimizing pH problems and excessive electron donor usage, including use of injection-extraction wells, dual recirculation wells, and nested injection-extraction wells.     

Biodiversity and ecogeography of wild Lactuca spp. in some European countries

Staff members of the Department of Botany of Palacký University in Olomouc and Gene Bank Department – Workplace Olomouc, Research Institute of Crop Production in Prague, Czech Republic, conducted an expedition in seven European countries (Austria, Czech Republic, France, Germany, Italy, the Netherlands, Switzerland) in August/September 1999 to collect wild Lactuca spp. germplasm and study its geographic distribution, ecology and biodiversity. During the mission, more than 600 locations were visited resulting in the collection of 602 seed samples (accessions) of wild Lactuca species and 13 seed samples of related genera (Chondrilla and Mycelis). Lactuca serriola f. serriola, L. serriola f. integrifolia, L. saligna and L. viminea subsp. chondrilliflora were prevalent in southern Europe (Italy, France), however, only L. serriola was common in central and western Europe (Austria, Czech Republic, Germany, Netherlands, Switzerland). The greatest diversity of Lactuca species was found in France, where also the most seed samples (165) were collected. The most characteristic habitats with a high density of Lactuca spp. populations were observed along roads and highways, grassy ditches, ruderal communities, and dust-heaps. Natural infections by powdery mildew (Erysiphe cichoracearum) and downy mildew (Bremia lactucae) on some wild Lactuca spp. were observed. Recent observations concerning the geographic distribution, population structure, habitats, and natural occurrence of diseases of Lactuca spp. are discussed. This assemblage of genetic resources of Lactuca spp. can serve as the basis of future studies of species diversification, spatial population structure, plant microevolution, domestication processes, and genetic variability of host-parasite interactions.     

Increase in auxiliary photoprotective photosynthetic pigments in wheat seedlings induced by Azospirillum brasilense

Inoculation of wheat seedlings with the plant growth-promoting bacterium Azospirillum brasilense Cd was immobilized in alginate microbeads and, without applying any stress, significantly increased the quantity of several photosynthetic pigments, such as chlorophyll a, chlorophyll b, and the auxiliary photoprotective pigments violaxanthin, zeaxanthin, antheroxanthin, lutein, neoxanthin, and β-carotene. This resulted in greener plants with no apparent visible stress. After monitoring the quantity of photosynthetic pigments for 4 weeks, we observed that inoculated plants had higher quantities of pigments in shoot and stem. The greatest difference in the quantity of all pigments between inoculated and noninoculated plants occurred in the first week of growth. Regardless of treatment, the quantity of pigments in stems was three to four times less than the quantity of these pigments in shoots. Application of Azospirillum, either as liquid inoculant or as alginate microbeads, did not alter the positive effect of the bacteria on pigment production or the positive response of the plants towards A. brasilense Cd inoculation.     

In-situ enrichment and analysis of atrazine-degrading microbial communities using atrazine-containing porous beads

We examined the community composition of microbes that colonized atrazine-containing beads buried in agricultural soils that differed in atrazine treatment history. Bacterial abundance was 5-40-fold greater in atrazine-fortified beads. In beads containing 20 mg atrazine kg⁻¹ buried in soil with a history of atrazine application (conditioned soil), the abundance of Actinobacteria increased approximately 80-fold whereas in control soil, Actinobacteria were enriched only 10-fold and the gamma-Proteobacteria and Planctomycetes increased by 60- and 25-fold, respectively. The gamma-Proteobacteria were enriched by 120- and 230-fold in beads containing 200 mg atrazine kg⁻¹ in conditioned and control soil, respectively. The results demonstrate that BioSep^® beads are a suitable matrix for recruiting a diverse subset of the bacterial community involved in atrazine degradation.