左旋咪唑对鸡传染性法氏囊病疫苗免疫效果影响的观察

为观察左旋咪唑对鸡传染性法氏囊病疫苗免疫效果的影响，进行2个实验：试验1，饮水给予10mg/kg.d左旋咪唑，连用3d，然后进行鸡传染性法氏囊病疫苗初次免疫，免疫后14－21d，鸡血清抗IBDV抗体滴度明显降低；试验2，鸡在初次免疫后35d进行第2次免疫，且在2次免疫前先应用10mg/kg.d左旋咪唑，连用3d，免疫后鸡血清抗IBDV抗体滴度无明显变化。     

甲硝唑对鸡传染性法氏囊病活疫苗免疫效果的影响

本研究观察了药物对传染性法氏囊病（IBD）活疫苗初次免疫和二次免疫的影响，结果显示高抗传染性法氏囊病病毒（IBDV）母源抗体雏鸡IBD活疫苗初次免疫前应用10－100毫克／千克甲硝唑和10毫克／千克左旋咪唑，在免疫鸡血清抗IBDV抗体水平明显下降，甲硝唑用药鸡疫苗二次免疫后，血清抗IBDV抗体滴度明显高于未用药对照组。10毫克／千克左旋咪唑给药组疫苗二次免疫后鸡血清抗IBDV抗体滴度与未用药对照组无明显差别。     

甲硝唑对鸡传染性法氏囊病活疫苗免疫效果的影响

本研究观察了药物对传染性法氏囊病(IBD)活疫苗初次免疫和二次免疫的影响,结果显示高抗传染性法氏囊病病毒(IBDV)母源抗体雏鸡IBD活疫苗初次免疫前应用10～100毫克/千克甲硝唑和10毫克/千克左旋咪唑,在免疫鸡血清抗IBDV抗体水平均明显下降,甲硝唑用药鸡疫苗二次免疫后,血清抗IBDV抗体滴度明显高于未用药对照组.10毫克/千克左旋咪唑给药组疫苗二次免疫后鸡血清抗IBDV抗体滴度与未用药对照组无明显差别.     

缓释复方免疫增强剂对鸡传染性法氏囊病疫苗增强效果研究

100羽20日龄海兰褐蛋雏鸡随机均分为试验组和对照组,均肌肉注射IBDB87疫苗0.5mL/羽,同时给试验组雏鸡投服缓释复方免疫增强剂1粒/羽。在免疫前、免疫后10、20和30d,每组随机采血20羽,测定IBD抗体阳性率、T淋巴细胞ERFC形成率、脾脏和法氏囊指数。结果2组鸡上述测定指标具有相同的消涨趋势,且试验组显著或极显著高于对照组,表明缓释复方免疫增强剂可提高鸡体液和细胞免疫功能,促进免疫器官生长发育。     

缓释复方免疫增强剂对鸡传染性法氏囊病疫苗免疫增强效果研究

将100只20日龄海兰褐蛋雏鸡随机分成试验组和对照组．分别肌肉注射IBDB87疫苗0．5mL／只,同时给试验组雏鸡投服缓释复方免疫增强剂l粒／只．对照组鸡不投服．在免疫前和免疫后10、20、30d,每组随机采血．测定鸡IBD抗体阳性率、T淋巴细胞ERFC形成率、脾脏和法氏囊指数。结果表明．免疫后不同时期鸡IBD抗体阳性率、T淋巴细胞ERFC形成率、睥脏和法氏囊指数试验组显著或极显著高于对照组．说明缓释复方免疫增强剂可以提高鸡体液免疫功能和细胞免疫功能．促进免疫器官生长发育．     

缓释复方免疫增强剂对鸡传染性法氏囊病疫苗免疫增强效果研究

鸡传染性法氏囊病（IBD）是鸡的一种急性、高度接触性传染病，防治主要应用弱毒苗和灭活苗，但由于病毒变异和超强毒株出现，病原体混合感染，其它疾病引起免疫抑制，应激因素的刺激等，造成鸡IBD疫苗免疫效果不佳甚至失败。如何提高IBD疫苗的免疫效果已成为研究的热点。陆钢等（1995）在雏鸡免疫ND和IBD疫苗前后，以0．2％蜂花粉拌料，肌注芦荟提取液0．1mL／只，在二免后1个月时，ND和IBD效价较对照组提高1个滴度，差异极显著。     

鸡传染性法氏囊病疫苗B87对SPF鸡289的免疫效果

30日龄SPF鸡经点鼻途径用鸡传染性法氏囊病病毒（1BDV）的中等毒力疫苗B87株免疫后14d，再以200个ELD50超强毒IBDV株GX8/99点鼻人工攻毒，所有鸡都没有发病死亡。而对照鸡有10/10发病和6/10死亡，并有典型的病理变化。这表明，对SPF鸡用中等毒力的IBDV疫苗免疫可诱发有效的免疫保护作用。并能完全抵抗超强毒IBDV的攻击。     

鸡传染性贫血病毒对鸡传染性囊病活疫苗(Gt株)免疫效果的影响

鸡传染性贫血(CIA)是由鸡传染性贫血病毒(CAV)引起的一种以再生障碍性贫血和淋巴器官组织萎缩为主要特征的免疫抑制病.崔现兰(1992)首次于我国东北地区分离到该病毒[1],目前在我国许多地方都有该病毒的报道.CAV侵入雏鸡体内后,主要作用于骨髓和胸腺等淋巴器官,导致感染鸡出现再生障碍性贫血及胸腺等淋巴器官严重萎缩,产生免疫抑制,对禽病疫苗的免疫应答机能降低,甚至丧失.为研究鸡传染性囊病病毒对鸡传染性囊病活疫苗(Gt株)免疫效果的影响,本试验采用人工感染CAV的方法研究CAV感染对鸡传染性囊病活疫苗(Gt株)免疫效果的影响.     

法氏囊提取液对新城疫活疫苗免疫效力的影响

本文对鸡法氏囊提取液与ND活疫苗的使用方法进行了分析。通过血凝抑制抗体滴度的测定结果表明,预先接种鸡法氏囊提取液时,经肌肉注射、口服和点眼滴鼻接种方式均能提高ND疫苗免疫鸡的抗体产生能力,且经肌肉注射法最佳。但是,在整个试验期间,法氏囊提取液只能增强鸡群对ND活疫苗的早期免疫应答,而法氏囊提取液与ND活疫苗同时使用时更能刺激免疫鸡群的早期抗体产生水平。     

鸡传染性法氏囊病免疫复合物疫苗的安全性和免疫效力试验

用鸡法氏囊病免疫复合疫苗(IBDV-Icx)和常规IBD活疫苗分别免疫1日龄商品鸡和SPF鸡,进行疫苗的安全性及免疫效力比较.免疫后8d检测,IBDV-Icx免疫组鸡的法氏囊未见明显萎缩,而传统弱毒疫苗免疫组鸡的法氏囊萎缩明显;免疫后28d各组用IBDV标准强毒株进行攻击,IBDV-Icx免疫组鸡的攻毒保护率均为10/10,常规IBD活疫苗对照组分别为8/10及9/10.免疫后3个月,IBDV-Icx免疫组血清抗体可达AGP1∶32,攻击强毒仍为10/10保护,而常规IBD活疫苗免疫后2个月抗体AGP为0,攻毒保护率为1/10,免疫后3个月时攻毒10/10发病.     

鸡传染性法氏囊病的诊断

鸡传染性法氏囊炎病是一种主要危害鸡的体液免疫中枢器官——法氏囊的病毒性传染病。牡丹江市某大型养鸡场2006年10月,购自某种鸡场海     

鸡传染性法氏囊病的防制经验

鸡传染性法氏囊病（IBD）是由双1KNA病毒科的传染性法氏囊病毒（IBDV）引起幼鸡的一种急性、高度接触性传染病。病鸡表现为精神萎顿、食欲不振、下痢、振颤和衰竭。剖检以脱水、骨骼肌出血、肾小管尿酸盐沉积和法氏囊肿大、出血、浆膜面覆盖有淡黄色干酪样或奶油样渗出物为特征。该病主要侵害法氏囊，具有发病骤然、病程短、高发病率、低死亡率的特点，是养鸡场鸡群发病最频繁并造成养鸡业重大经济损失的主要疾病之一。     

Studies on a vaccine against infectious bursal disease

An infectious bursal disease vaccine, registered for use in breeder flocks, was studied for efficacy on the day-old offspring of vaccinated hens and for virulence in susceptible day-old and 6-week-old chickens. When given to susceptible day-old chicks and 6-week-old cockerels, the vaccine was found to induce atrophy and pathology of the bursa of Fabricius similar to that observed in field infections. Chicks vaccinated at day-old had markedly lowered titres in the haemagglutination inhibition test to Newcastle disease virus, when this was given 2 weeks later, but the response of the 6-week-old cockerel was similar to that of control birds. Maternal antibody induced by the vaccine protected chicks against infection at day-old.     

夏季谨防鸡法氏囊炎

近年来,每当进入5月份以后,随着北方温度的升高,养户们开始大量饲养和进购鸡雏.鸡法氏囊炎的发病率开始逐渐增多,在7～8月份达到发病高峰,10月份以后随着温度、湿度的降低,发病率又呈逐渐下降的趋势.     

Effect of infectious bursal disease virus infection on energy metabolism in embryonic chicken livers

ABSTRACT

1. The purpose of this study was to investigate ATP levels and the activities of important enzymes involved in glycolysis and TCA cycle in livers of embryonated chicken eggs infected by infectious bursal disease virus (IBDV).

2. Embryonated chicken eggs (9 days) were randomly divided into two groups (50 eggs per group). The first group was inoculated with a very virulent IBDV (vvIBDV) isolate into the chorioallantoic membrane. The second group was maintained as uninfected control eggs and inoculated with physiological saline. Embryo survival was assessed daily, and six embryos were sacrificed at 24, 48, 72, 96, and 120 hpi for examining livers. Viral loads in the livers were evaluated by qRT-PCR. A comparative analysis of markers associated with the regulation of energy metabolism across several functional classes (ATP, pyruvic and lactic acids, mitochondrial protein, NAD+/NADH ratios, and enolase, lactic acid dehydrogenase and the respiratory chain complex I activities) were examined in the context of IBDV infection.

3. The results indicated that increases in the enzymatic activities associated with glycolytic metabolism in turn affected the synthesis and cytoplasmic concentrations of ATP at early timepoints in infected chicken embryos. Subsequently, energy metabolism was inhibited through the pathological perturbations of metabolic enzymes and mitochondrial damage, as inferred from reduced ATP generation.

4. These results suggested impaired bioenergetics, which may lead to liver dysfunction consequent to IBDV infection, contributing to the disease pathogenesis.