青海省共和县绵羊衣原体病的血清学调查

衣原体病是由鹦鹉热衣原体（Chlamydia Psittaci）感染引起的羊、牛、人等多种动物共患的传染病,鹦鹉热衣原体属于衣原体科、衣原体属。广泛寄生于人类、鸟类、哺乳动物体内,它可引起不同种动物的多征后群疾病,临诊病理特征为流产、肺炎、肠炎、结膜炎、多发性关节炎和脑炎。该病又叫地方性流产病、衣原体性流产。近几年的研究结果表明,随着布氏杆菌病的基本控制,衣原体病和弓形虫病已成为造成牛羊流产的主要传染性因素。目前青海省对于该病的血清检测已在全省范围内广泛展开,对于检测结果各州都有报道,而本县未见此类报道。为此笔者于2013年5月份应用血凝抑制试验对本县牛羊中该病的感染情况进行了血清学调查,现将结果报告如下：     

高寒牧区藏系绵羊衣原体病血清学调查

<正>衣原体病(Chlamydiosis)又称地方流行性流产,是由鹦鹉热衣原体(Chlamydia psittaci)引起的一种在多种动物间广泛传播的以流产(妊娠后期)、早产、死胎或产下弱羔及发热、肺炎、肠炎、结膜炎、多发性关节炎、脑脊髓炎等为主要特征的人畜共患性传染病。据兴海县畜牧兽医站调查显示,2008年全县绵羊流产率达4.84%,将流产的原因归结为布氏     

绵羊地方流行性流产的临床诊断

绵羊地方流行性流产是由绵羊鹦鹉热衣原体引起的亚急性接触性传染疾病。临床症状以发热，流产、死产或产下弱羔为典型特征，病理学特征为坏死性胎盘炎、急性乳腺炎、溃疡性子宫内膜炎。1病原绵羊地方流行性流产一般由鹦鹉衣原体引起，病原为免疫I型绵羊衣原体。其具有鹦鹉热衣原体的特征，用姬姆萨染色时成熟的颗粒呈紫红色。该病原能在5～7日龄的鸡胚卵黄囊中迅速生长，密度高，     

羊衣原体病的临诊类型与防治措施

羊衣原体病是由鹦鹉热衣原体引起的山羊、绵羊的一种传染病。临床上以发热、流产、死产和产出弱羔为特征。     

一起山羊流产衣原体感染的诊断

陕西省某山羊养殖场发生了一起山羊流产病,经流行病学调查,临床症状和病理剖检观察进行初步诊断;利用布鲁菌虎红平板凝集试验、衣原体间接血凝试剂盒对10份流产母羊血清检测;对病料进行细菌学检测;依据GenBank收录的山羊流产性衣原体基因组设计1对特异性引物,通过PCR方法从病料中扩增衣原体特异性片段。结果表明,根据临床症状和病理变化初步怀疑为布鲁菌和鹦鹉热亲衣原体感染;10份血清检测结果为布鲁菌病血清全部阴性,衣原体感染血清全部为阳性;细菌学检测结果为阴性;PCR结果获得523bp基因片段,测序结果与鹦鹉热亲衣原体100%相似。依据流行病学调查,临床症状和病理剖检观察,病原学和血清学诊断,最终确诊该病为鹦鹉热亲衣原体引起的山羊地方流行性流产。     

青海省牛羊衣原体病血清学调查

<正>牛羊衣原体病是由鹦鹉热衣原体引起的一种人畜共患病,临床特征是流产、脑炎、肠炎、结膜炎、多发性关节炎等。被感染母牛的主要症状是流产、早产和产死胎,绵羊主要表现为流产和肺炎,山羊表现为肺炎及多发性关节炎。本病分布于世界各地,我国的西北、内蒙古、新疆等地都有发生和流行,给畜牧业经济和公共卫生造成很大影响[1]。牛羊衣原体病的防控虽不像重大动物疫病一样被列入国家重点防治计划,但在实际生产中却具有较大的现实意义。以前曾有     

山羊流产原因及其综合防治

1 羊的地方性流产病(即羊流产衣原体病)衣原体病不论山羊、绵羊还是其他动物(包括人)均可感染,特别是绒山羊感染率最高.当怀孕的羊感染衣原体病后,衣原体在胎衣特别是绒毛叶生长繁殖,引起患部发炎,导致胎羔早期产出.种公羊感染后因精液带衣原体,借配种机会易传染给母羊.衣原体是介于病毒与细菌之间的一种微生物,其形状为球形.     

如何预防羊衣原体病

<正>羊衣原体病是由鹦鹉热衣原体引起的绵羊、山羊的一种传染病。临床上以发热、流产、死产和产出弱羔为特征。在疾病流行期,也见部分羊表现多发性关节炎、结膜炎等疾患。流行特点。鹦鹉热衣原体可感染多种动物,多为隐性经过。家畜中以牛、羊较为易感,禽类感染后称为"鹦鹉热"或"鸟疫"。许多野生动物和禽类是本菌的自然贮主。患病动物和带菌动物为主要传染     

南非绵羊与牛的衣原体病

南非在1971—1977年期间,绵羊与牛的衣原体病流行,曾有过简报。在绵羊与牛中分出了强病原物鹦鹉热衣原体(Chlamydiapsittaei)。尽管如此,牛与绵羊接触中,在种间没有具备传播的条件,摄入污染物为主要的感染途径,而肠道带菌与虫媒的作用也要考虑。绵羊地方性流产与伴有并发症的新生弱羔呈神经症状,或发生4—5周令羔羊的肺炎。衣原体初次感染的羊群中,流产率高达60％,在慢性感染群中成为散发性流产。尸体剖检时病理变化明显,并被详述。农场中新生幼畜的并发病有关节损害、肺炎、腹泻等。这种病在经济上有深远的意义。许多病例中分离出鹦鹉热衣原体,也并发其他病原     

羊衣原体病的实验室诊断

羊衣原体病是由鹦鹉热衣原体引起的一种亚急性传染病,绵羊和山羊均易感,无明显的品种差异。临床上以流产、产弱胎或死胎、结膜炎、肺炎为主要特征。其中绵羊地方性流产、山羊地方性流产、绵羊多发性关节炎等病流行较为严重。目前,我市永安镇某养羊大户出现了绵羊流产、...     

贵州省山羊布鲁氏杆菌病衣原体病蓝舌病的血清学调查研究

为了解贵州省山羊3种流产疫病流行情况,分别从贵州省4个地区共采集194份山羊血清,采用虎红平板凝集试验法(RBT)对194份血清进行布鲁氏杆菌病抗体检测,平均阳性率为0%;采用间接血凝试验法(IHA)对194份山羊血清进行衣原体病抗体检测,平均阳性率为5.7%;采用酶联免疫吸附试验法(ELlSA)对178份山羊血清进行...     

鹦鹉热衣原体疫苗研究进展

文章叙述了鹦鹉热衣原体的生物特性以及感染的宿主范围;鹦鹉热衣原体减毒活疫苗温度敏感株的培育及致病机理的研究;灭活疫苗灭活条件的研究,最佳免疫量,不同免疫途径的研究和我国对绵羊和猪鹦鹉热衣原体灭活疫苗的研究;以及鹦鹉热衣原体主要外膜蛋白基因工程亚单位疫苗和禽衣原体DNA疫苗的研究情况。     

Rapid detection of Chlamydia psittaci in vaginal swabs of aborted ewes and goats by enzyme linked immunosorbent assay (ELISA)

An enzyme linked immunosorbent assay (ELISA) for the detection of Chlamydia psittaci in vaginal swabs of aborted ewes and goats has been developed using microtiter plates coated with sheep anti-Chlamydia immunoglobulin G. This technique was compared to the direct isolation of the agent by plaque assay on McCoy cells. Among 89 specimens from animals in infected flocks, 58 were positive by both methods, seven were only positive by ELISA, and nine others were only positive by direct isolation (plaque assay). None of the 75 specimens from animals in healthy flocks gave a positive response in ELISA or the plaque assay. Unlike direct isolation in cell culture, the ELISA technique permitted the detection of Chlamydia even in the absence of special care in sampling and conservation of specimens.     

Seroprevalence of Chlamydia psittaci-specific antibodies in small stock in Namibia--epidemiological study with an enzyme-linked immunosorbent assay (ELISA)

An IgG (H+L)-ELISA was applied as a screening test for antibodies against Chlamydia (C.) psittaci in sera of goats and sheep in Namibia. In 576 (27.3%) of a total of 2,107 sera (299 = 25.2% of 1,185 caprine and 277 = 30.0% of 922 ovine sera) chlamydial antibodies could be detected. 86% of all farms tested revealed seropositive animals. Chlamydial infections were prevalent in all the geographical regions tested. The infection rates per State Veterinary District varied from 12.0% (Otjiwarongo) to 50.0% (Otavi) in goats and from 13.3% (Otjiwarongo) to 41.7% (Windhoek) in sheep. The regional distribution of chlamydial infections was not related to geographical or climatic factors. Sera from herds showing symptoms indicative for chlamydial infections showed significantly higher antibody rates (35% in goats and 39% in sheep) than sera from herds without health problems (18% in goats and 24% in sheep). Considering only sera from farms with clinical history of chlamydiosis, high seroprevalences were correlated to the symptoms abortion and keratoconjunctivitis. As in other countries, enzootic abortion seems to be the main manifestation of chlamydial infection in small ruminants in Namibia. C. psittaci might also play a considerable role in the etiology of infectious keratoconjunctivitis, whereas association with other clinical entities seems to be rare.     

Abortion in small ruminants in Switzerland: investigations during two lambing seasons (1996-1998) with special regard to chlamydial abortions

Abortion cases of 144 goats und 86 sheep were investigated etiologically during 2 lambing seasons (1996/1997, 1997/1998). Macroscopic inspection of fetus and placenta was completed by histopathology and bacteriological isolation of agents. In addition, immunohistologically the following antigens were labeled in formalin-fixed and paraffin-embedded tissue sections: Toxoplasma gondii, Neospora caninum, Chlamydophila abortus (formerly Chlamydia psittaci serovar 1) and Border Disease Virus. From farms with abortions caused by Chlamydophila abortus specific data were recorded. In 75% of abortion cases in sheep and in 59% of cases in goats an etiologic diagnosis could be substantiated. Chlamydophila abortus is the most commonly involved agent in the etiology of caprine and ovine abortion (sheep 39%, goats 23%), followed by Toxoplasma gondii (sheep 19%, goats 15%) and Coxiella burnetti (sheep 1%, goats 10%). All other agents are of minor importance. An infectious cause of abortion based on histopathologic findings without isolation of agents was observed in sheep (10%) and goats (21%). Malformation occurred in sheep (2%) and goats (3%) and lesions suggestive for Vitamin E/Selenium deficiency were seen in goats only (2%).     

Chlamydial infections in small ruminants.

Chlamydophila abortus (formerly Chlamydia psittaci) is one of the most important causes of reproductive failure in sheep and goats, especially in intensively managed flocks. The disease is usually manifested as abortion in the last 2 to 3 weeks of gestation, regardless of when the animal was infected. Ewes that abort are resistant to future reproductive failure due to C. abortus, but they become inapparent carriers and persistently shed the organism from their reproductive tracts during estrus. Chlamydophila pecorum is the other member of the genus that affects small ruminants, and it is recognized as a primary cause of keratoconjunctivitis in sheep and goats and of polyarthritis in sheep.     

应用核酸杂交技术检测畜禽衣原体病的研究

从纯化山羊流产衣原体颗粒提取ＤＮＡ，用ＤＮＡ限制性内切酶切割，进行酶切图谱分析，与文献报道的图谱对比，证实确系纯的衣原体ＤＮＡ制品。将衣原体ＤＮＡ用光敏生物素标记，制成核酸探针，用斑点杂交法检测衣原体核酸，灵敏度可达１０ｐｇ。又用重组克隆技术将衣原体ＤＮＡ片段克隆于大肠杆菌质粒载体上，筛选出衣原体特异的ＤＮＡ片段制成光生物素探针，可以同样有交效地检测衣原体核酸。用光生物素衣原体探针检测了山羊、绵羊、豚鼠、小白鼠、鸡胚等的衣原体感染病料，结果准确。与间接血凝法检测衣原体感染做了对比试验，证明核酸杂交法检测衣原体病更为灵敏和特异     

A simple staining method for the identification of chlamydial elementary bodies in the fetal membranes of sheep affected by ovine enzootic abortion.

A dark-ground methylene blue (DGMB) staining method was used to demonstrate chlamydial elementary bodies in fetal membranes of sheep affected by Chlamydia psittaci. Before evaluation on material from clinically affected animals, the DGMB method was compared with modified Ziehl-Neelsen (MZN) and dark-ground Giemsa (DGG) staining methods for its ability to demonstrate chlamydial elementary bodies in hens' eggs which had been experimentally infected with C. psittaci. DGMB was more specific in its staining of chlamydial elementary bodies than DGG or MZN. The DGMB method was found to be a more reliable technique for the examination of fetal membranes from sheep affected with C. psittaci than DGG or MZN. Those samples diagnosed as positive using the DGMB showed a good correlation with those diagnosed as positive on macroscopic examination.     

Isolation of Toxoplasma gondii from goats with history of reproductive disorders and the prevalence of Toxoplasma and chlamydial antibodies

The prevalence of antibodies to Toxoplasma gondii and Chlamydia psittaci was assessed in goats with a history of abortion, stillbirth and neonatal mortality. Antibodies were detected in 540 (30%) and 57 (3.2%) goats out of 1799 tested by indirect haemagglutination and complement fixation tests, respectively. Toxoplasma gondii was isolated for the first time in Botswana from 22 out of 81 sets (27.2%) of foetal tissues, maternal and foetal cotyledons and uterine tissues of goats which had previously aborted or given birth to stillborn or weak kids that died within two days of birth. These results implicate T. gondii and C. psittaci, but especially the former, to be associated with caprine reproductive problems and require appropriate control measures.     

Chlamydia abortion in sheep: possibilities for serological diagnosis using a competitive ELISA and insight into the epidemiologic situation in Switzerland]

466 sheep sera out of 19 flocks in Switzerland were examined by a competitive enzyme linked immunosorbent assay (cELISA) for antibodies against Chlamydia psittaci "serotype 1" ("ovine enzootic abortion"). Since numerous positive reactors were found in flocks without abortion history, 30 fecal samples out of two of these flocks were examined by PCR for evidence of chlamydial DNA. One of these samples turned out to contain DNA of Chlamydia psittaci "serotype 1". These results suggest, that in Switzerland "serotype 1" of Chlamydia psittaci is widespread not only as cause of chlamydial abortion but also as latent intestinal infection in sheep. The resulting difficulties for serological diagnosis of chlamydial abortion and possible solutions based on the cELISA are discussed. The complement fixation test (CFT), still considered as standard method for serological examination for Chlamydiae, has additionally been applied.