采前喷施脱落酸对欧李果实品质的影响

为探究采前喷施脱落酸(ABA)对欧李果实品质的影响,本研究选择农大6号和农大7号2个欧李品种为试验材料,在喷施不同浓度和不同次数的ABA后测定成熟果实的单果重、可溶性固形物含量、可滴定酸含量、类黄酮含量、总酚含量以及抗氧化能力。结果表明,喷施不同浓度和不同次数的ABA后,农大6号欧李果实可滴定酸含量、类黄酮含量、总酚含量以及抗氧化能力降低,对于可滴定酸含量而言,喷施2次比喷施1次的降酸效果明显,而类黄酮含量、总酚含量以及抗氧化能力受ABA浓度和喷施次数的变化影响不大。各处理下农大7号欧李果实的单果重和可溶性固形物含量均得到了升高,类黄酮含量、总酚含量以及抗氧化能力受ABA浓度和喷施次数的变化影响较大。根据主成分分析进行排名可知,不同浓度不同次数的脱落酸处理后农大6号欧李果实综合评分均低于对照,果实综合品质下降,农大7号欧李果实综合评分均高于对照,当脱落酸浓度为25 mg·L^-1,喷施次数为1次时对果实综合品质的提升效果最好。本研究为提升欧李果实品质选取喷施适宜浓度和次数的ABA提供了理论基础。     

脱落酸对番茄部分果实性状和营养品质的影响

为研究脱落酸(ABA)对番茄果实品质的影响,本研究测定了番茄ABA生物合成缺失突变体not和flc及其野生型各成熟时期果实,以及外源100 μmol·L^-1 ABA处理后不同天数的番茄果实的外观品质和营养品质。结果表明,内源ABA缺失抑制番茄果实增重并促进果实纵向生长,但对果实硬度无明显影响。同时,内源ABA可影响番茄果实可溶性固形物含量并促进破色期和转色期的还原糖积累。not和flc果实中番茄红素、β-胡萝卜素、叶黄素和总类胡萝卜素含量均显著高于其野生型,且外源喷施试验表明,与对照组相比,外源100 μmol·L^-1 ABA处理能显著抑制番茄果实中番茄红素、β-胡萝卜素和总类胡萝卜素的积累。综上,ABA在调控番茄果实外观品质方面,可促进番茄果实增重并抑制番茄果实纵向生长;在调控番茄果实营养品质方面,可促进还原糖积累并抑制类胡萝卜素积累。本研究结果为利用ABA调控番茄果实品质提供了一定的理论依据和技术支持。     

Abscisic acid improves tomato fruit quality by increasing soluble sugar concentrations

Abscisic acid (ABA) is crucial in fruit maturation and senescence and is considered as the other ripening control factor other than ethylene. Important components in ripening fruit are soluble sugars, which make the fruit sweeter and carotenoids, important flavor compounds in ripened fruit. The purpose of this study was to examine the effects of foliar ABA and calcium (Ca) fertilizer treatments (individually and in combination) on determinate beef-steak greenhouse tomato (Solanum lycopersicum) leaf chlorophylls and carotenoids, fruit carotenoids, and soluble sugar concentrations. Foliar spray treatment of 500 mg L^?1 ABA increased zeaxanthin (ZEA) and β-carotene (BC) in tomato leaf tissue. Increases in Ca fertilizer treatments significantly decreased tomato leaf violaxanthin (VIO), but no effect on other carotenoids. The application of 500 mg L^?1 ABA foliar spray significantly increased glucose and fructose concentrations in tomato fruit tissue. Foliar application of ABA treatments can increase overall chlorophyll, carotenoid content, and fruit quality.     

Effect of soil application of cadmium contaminated lime on soil cadmium distribution and cadmium concentration in strawberry leaves and fruit

Heavy metal environmental pollution which occurs as a result of lime contaminated with cadmium (Cd) poses a potential health hazard. This investigation was undertaken to study uptake of Cd by strawberry plants grown in soil amended with three different sources and two different rates of industry waste lime containing 3.4, 14.3, and 60.0 mg Cd/kg, respectively. The effects of Cd applied to the soil were investigated, including its distribution in the soil and effect on Cd concentration in strawberry cv. Senga Sengana (Fragaria anassa) leaves and fruit in response to soil organic matter content and lime rates. Cadmium accumulated mainly in the plough layer, increasing from 0.170 mg Cd/kg (background level) to a maximum of 1.2 mg Cd/kg. Fruit had very high, hazardous Cd concentrations regardless of its content in the soil. This indicates that Cd was easily taken up by strawberry plants and accumulated in upper plant parts, including the fruit. Soil Cd content had no effect on concentration of this element in strawberry fruit. However, plant Cd uptake and fruit concentration was increased in acid soils even when soil Cd concentration was low.     

丛枝菌根真菌对NaCl胁迫下番茄内源激素的影响

采用盆栽试验研究了不同浓度NaCl（03%、06%和10%）胁迫下,接种丛枝菌根真菌Glomus mosseae-2 对番茄内源激素的影响。结果表明：（1）盐胁迫下,植株生长受抑,生长促进物质IAA、GA3和Zeatin含量下降,生长抑制物质ABA含量增加,接种Glomus mosseae-2增加了植株干物质量和这些激素的含量;（2）菌根形成过程中,Glomus mosseae-2参与调节内源激素平衡, 降低了叶片ABA/IAA、ABA/GA3、ABA/Zeatin及ABA/ (IAA+GA3+Zeatin)的比值; （3）气孔导度（gs））和ABA/Zeatin值呈极显著负相关,同一盐浓度胁迫下,接菌株有较高gs和较低的ABA/Zeatin值。 ABA和Zeatin共同调节气孔对盐胁迫的响应,维持接菌株较高的气孔导度,增强了番茄的耐盐性。     

磷锌配施对花生生理特性、产量及品质的影响

协调磷-锌配比是作物达到最佳产量和质量的保障。采用大田试验,在豫南花生主产区高磷低锌的砂姜黑土上,研究了不同磷、锌配比对花生抗氧化特性及产量、品质的影响。结果表明,磷锌配施对花生生理特性产生一定影响,相同磷用量下,施锌比不施锌显著提高了花生叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、生长素、可溶性糖和可溶性蛋白质含量,降低花生叶片丙二醛(MDA)含量;相同锌用量下,随磷用量的增加,花生叶片的SOD和POD活性先增高后降低,MDA含量先降低后增加,生长素含量无显著变化,可溶性糖和可溶性蛋白质含量也表现出先增高后降低的趋势,但处理间差异呈显著或不显著。对花生产量和品质来讲,与不施肥相比,磷锌单独或配施均在一定程度上提高了花生产量、粗脂肪及脂肪酸组分,且磷锌配施效果优于二者单独施用,其中90 kg/hm~2磷肥与60 kg/hm~2锌肥配施处理的产量最高,增产率达24.1%,增产原因可能是提高了花生的抗氧化能力,增加了单株饱果数和饱果重。双因素方差分析结果显示,磷对花生株高、侧枝长、单株饱果数、单株饱果重和产量影响显著;锌仅对单株饱果数和饱果重及产量影响显著;磷-锌交互作用对花生株高影响显著,对产量及其构成因素和品质影响不显著。综上,磷锌配施可以改善花生的生理特性,提高花生产量和品质,但高量磷与锌肥配施却不利于花生抗氧化能力、产量和品质的提高。在豫南花生主产区,建议多补充锌肥,少用磷肥。     

Interactions of Salinity Stress and Flower Thinning on Tomato Growth,Yield, and Water Use Efficiency

This study was conducted to investigate the effects of salinity stress and flower number on growth, yield, water use efficiency (WUE), and fruit quality of cherry tomatoes cultivated under soilless conditions. The experiment was conducted in a plastic house (5-m wide × 11-m long) located in Gifu University. The seedlings were transplanted in a randomized complete block design with six plants per treatment (NT1 and ST1 were with four plants), giving a total of 44 plants in 22 pots (two plants per pot). Two different salinity levels [no-salinity and salinity with electrical conductivity: 0.8 and 3.0 dS m^?1, respectively] and four flower number treatments (8, 13, 18, and free per truss) were investigated in the experiment. The results showed that salinity stress negatively affected tomato growth, yield, and marketable yield, but improved tomato fruit quality. The number of flowers had no effect on tomato growth variables and WUE, but the yield significantly increased with increasing flower number. However, the fruit quality was decreased with increased flower number. A reasonable control for fruit load can increase marketable yield in commercial cultivation. Under salinity stress conditions, properly increasing the number of flowers can avoid yield reduction.     

Effects of abscisic acid and calcium on tomato fruit aroma volatiles

Aroma volatiles are derived from precursors, such as amino acids, fatty acids and carotenoids in tomato fruit. Volatiles enhance the main flavor components in the fruit, particularly sugars. Abscisic acid (ABA) is derived from the carotenoid pathway and there may be an indirect connection to this pathway. Therefore, the purpose of this study was to examine the influence of ABA on tomato fruit volatiles. This study identified five flavor volatile compounds that were consistently present in “Mt. Fresh Plus” tomato fruit tissue. They were 2-methyl furan, (E)-2-hexeanl, 1-hexanol, hexenal, and 6-methyl-5-hepten-2-one. ABA treatments did not have an effect on volatile concentrations in “Mt. Fresh Plus” tomato fruit. Majority of the volatiles identified did not differ between the ABA treated plants and the ABA control plants. However, ABA treatments did significantly decrease (E)-2-hexenal. These results indicated that ABA treatments did not have a major effect on the aroma volatile profile of the fruit.     

Mycorrhizal inoculation affects arbuscular mycorrhizal diversity in watermelon roots,but leads to improved colonization and plant response under water stress only

Horticultural crops in the Mediterranean basin have to cope with severe drought conditions. The effect of inoculating watermelon plants grown under limited water availability conditions with AM fungi on the fruit yield, water use efficiency (WUE), root-N and -P content was examined. We focused on the impact of watering level and inoculation with allochthonous AM fungi on the diversity and presence of AM fungi in the watermelon roots using molecular techniques. An open field experiment was conducted and plants were grown with (M) and without AM fungal inoculum (NM), subjected to water stress (NW) and no stress conditions (W). Suboptimal water application (NW) resulted in significant reduction of fruit yield, root-N and -P content. Inoculation of plants grown under water stress resulted in a significant increase of WUE (19%), fruit yield (19%), root-N (27%) and -P (40%). However, only root-P responded to AM inoculation under non water stress conditions demonstrating 23% increase in M plants. DNA extracted from root samples was subjected to PCR–DGGE analysis. The native mycorrhizal population colonized watermelon roots, as indicated by DGGE bands in NM treatments. Some members of this colonizer community appear sensitive to the introduction of allochthonous inocula and to water stress conditions. Cloning and sequencing of AM fungi revealed that watermelon roots were colonized by Glomus and Paraglomus species. A TaqMan real-time PCR assay was also carried out targeting the 18S rRNA gene for the quantification of AM nucleic acids. The 18S rRNA copy numbers of AM fungi were significantly increased in M plants compared to NM plants under water stress. On the contrary, under non stress conditions M and NM plants did not show significant differences, indicating that inoculation with AM fungi was related to the response of plants to water stress conditions. Principle coordinate analysis of the DGGE banding patterns showed that the diversity of AM fungal colonizers was strongly affected (i) by inoculation and (ii) by water stress in the inoculated plants. Inoculation affected fungal presence under water limitation conditions only. The latter was in line with the significant beneficial effect of inoculation on both WUE and yield only under water limitation.     

贵州本土半野生番茄砧木介导ABA生物合成信号通路调控植株耐旱性的机理研究

随着全球气候变暖,干旱胁迫成为限制番茄等蔬菜作物安全生产的重要因素之一。前期研究发现嫁接贵州本土半野生番茄GZ-01砧木其可以提高植株的耐旱性。为探究野生番茄GZ-01增强植株耐旱性的分子机理,以半野生番茄GZ-01砧木和红果番茄为试验材料,结合形态生理学和分子生物学,比较嫁接番茄和自嫁接番茄植株对干旱胁迫的响应。结果表明,干旱胁迫下,与自嫁接植株R/R相比,GZ-01/R嫁接植株细胞膜的损伤显著降低,植株的抗氧化能力、干物质累积量、CO2同化率和水分利用率显著提高,离体叶片失水率显著降低,下气孔闭合比率显著提高,脱落酸（ABA）合成相关基因表达量和ABA含量显著提高。嫁接植株GZ-01/R可能通过调控ABA的合成来影响气孔开闭,调控叶片失水率,提高植株水分利用率,从而影响植株对干旱胁迫的响应。本研究为开发利用贵州本土半野生番茄资源奠定了基础,为增强干旱胁迫和分子调控网络途径提供了理论支撑。     

干旱荒漠绿洲区葡萄根系分区交替灌溉试验研究

为了探讨西北旱区葡萄节水优质高效灌溉模式,于2004～2006年在甘肃河西走廊石羊河流域干旱荒漠绿洲区进行了田间试验,研究了根系分区交替滴灌、固定部分根区滴灌和常规滴灌三种灌溉模式对鲜食葡萄产量、品质和水分利用效率的影响。研究结果表明,根系分区交替灌溉灌水量较常规滴灌减少1/2,其产量未有显著下降,而且显著提高了浆果V c含量,使果酸含量降低,可溶性固形物含量显著提高,成熟度明显改善。根系分区交替灌溉的水分利用效率较常规滴灌提高了37.36%。采用根系分区交替灌溉可以达到大量节水、提高葡萄品质和成熟度而不明显降低产量的目的,可以作为西北旱区果园有效的节水优质高效灌溉模式。     

Underground communication of root hormones by common mycorrhizal network between trifoliate orange and white clover

Common mycorrhizal network (CMN) links up several coexisting plant individuals via underground root communication for resource sharing, while it is not known whether phytohormones participate in such communication. A two-chambered rootbox separated through 37-μm mesh was used to establish the CMN by Diversispora spurca between trifoliate orange (TO) and white clover (WC). After 20 weeks of inoculation, the CMN was established, as evident from high mycorrhizal buildup under supplier (mycorrhizal inoculation) TO–target (non-mycorrhizal inoculation) WC association. This CMN was eventually responsible for the significant increase in plant biomass of these associated plants. CMN decreased root abscisic acid (ABA) level in supplier and target plants, but increased root indole-3-acetic acid (IAA) level in supplier plant alone. CMN mediated significantly higher gibberellins (GAs) and zeatin riboside (ZR) concentration in roots of target plant, leaving supplier plant unaffected. Root ZR was bidirectionally transferred through the CMN. A higher root methyl jasmonate (MeJA) concentration was observed in supplier WC–target TO or supplier TO–supplier WC association, indicating an unidirectional communication from WC to TO. It suggested that except IAA, GAs, brassinosteroid (BR), and ABA, root MeJA and ZR were apparently involved in the underground communication by CMN.     

氮素对苹果果实内源激素变化动态与发育进程的影响

以8年生红富士/平邑甜茶(M.domestica Borkh.cv.RedFuji/M.hupenensis Rhed)盛果期树为试材,研究重疏果条件下土壤施氮对苹果果实发育影响。结果表明,花后15周前施氮处理与对照单果重无显著差异,由于对照果实比施氮处理果实早1周左右进入迅速膨大期,此后3～4周对照果实单果重高于施氮处理,但对照果实比施氮果实早1周左右停止生长。由于施氮延长了果实生长期,采收时施氮处理平均单果重高于对照。施氮对GA3、ZRs、ABA与IAA的含量及其变化动态有较大影响,特别在果实细胞膨大期;施氮显著提高了这4种激素的含量,但其峰值出现较对照晚1～2周,并且GA3、IAA、ABA含量峰值出现的时间比果实相对生长速率(RGR)峰值出现的时间略早。植物生长调节剂处理不施氮果实,使果实生长期延长,单果重增加。这些结果表明,氮素通过影响果实的激素水平调控果实的生长动态。果实发育进程的变化导致了有关品质指标的变化,主要表现为随施氮量增加,果实水分含量增加,果皮叶绿素与果肉可滴定酸浓度提高,果皮花青苷与果肉可溶性糖浓度降低。     

CHANGES IN ENDOGENOUS HORMONAL STATUS IN CORN (ZEA MAYS) HYBRIDS UNDER DROUGHT STRESS

Corn has several mechanisms that allow it to perceive the incoming water deficit and rapidly regulate its growth and physiology to cope with this stress. Among these regulated physiological responses, phytohormones, such as abscisic acid (ABA), gibberellin (GA₃), indole acetic acid (IAA), and zeatin (ZT) have important roles. To evaluate changes in hormonal status of corn hybrids under different drought stress levels, a greenhouse study was conducted at the College of Agriculture, Shiraz University, Iran, during summer 2010. Treatments consisted of drought stress in five levels: 100% field capacity (F.C.) as control, 80, 60, 40, and 20% F.C. and three corn hybrids, including single cross 704 (SC704), Maxima, and double cross 370 (DC370). Results showed that drought stress significantly affected phytohormones concentration, ABA concentration was increased and GA₃, IAA and ZT concentrations were decreased. Indeed, there was an initial increase (about 13.5% more than control) in IAA concentration. In severe drought condition, ABA concentration was eventually decreased (37.8% less than the first drought level, i.e., 80% F.C.). The ratios of GA₃, IAA and ZT to ABA concentration were also decreased. Among hybrids, SC704 showed the highest phytohormone concentrations and DC370 had the highest ratios of GA₃, IAA, and ZT to ABA concentrations. Overall, the results of this study suggest that although mild drought stress was associated with greater ABA concentration, it had negative impact on GA₃, IAA, and ZT concentrations. Furthermore, SC704 hybrid appeared to be more resistant to drought stress than the other hybrids.     

Exogenous applications of salicylic acid affect quality and yield of strawberry grown under antifrost heated greenhouse conditions

Abiotic stress is becoming more prevalent as the intensity of agriculture and the demand for farmable land are ever increasing. Besides drought and salinity stress, chilling or freezing stress is one of the most important limiting factors of crop production all around the world. Salicylic acid (SA) is a common plant‐produced signal molecule that is responsible for inducing resistance to a number of biotic and abiotic stresses. This study was conducted to determine the effect of foliar SA applications on fruit‐quality characteristics and yield of strawberry under antifrost heated greenhouse conditions in two successive experiments. Spraying of 1 mM SA (1 mM) was done once (SA1), twice (SA2), three times (SA3), or four times (SA4) during the vegetation period with 7 d intervals. Concentrations of chlorophyll and minerals were measured in leaves, and vitamin C (ascorbic acid), total soluble solids (TSS), titratable acidity (TA), and color (a*) in fruits. Fruit weight, early yield, and total yield were also determined. Foliar applications of SA positively affected TSS and ascorbic acid (AA) content of strawberry fruits. Salicylic acid treatments had no effect on TA of strawberry fruits. SA4 treatment gave the greatest a* in fruit and chlorophyll‐concentration values in the leaves. It was shown that SA treatments increased the content of all nutrients in the leaves of strawberry plants, and greater values often were obtained from SA3 and SA4 treatments. The early yield and total yield of strawberry were significantly affected by SA applications, among which SA3 and SA4 resulted in the highest early and total yields. The present study suggests that SA3 and SA4 treatments can ameliorate the deleterious effects of low temperatures on strawberry plants and that SA application may offer an economical and simple method for low‐temperature protection.     

Effect of three vesicular‐arbuscular mycorrhizae species and phosphorus on reproductive and vegetative growth of three strawberry cultivars 1

A study was undertaken to evaluate the yield, fruit size, and vegetative growth of three strawberry cultivars inoculated with three vesicular‐arbuscular mycorrhizal (VAM) species at three phosphorus (P) fertility levels. Vesicular‐arbuscular mycorrhiza inoculation and P fertility had no effect on inflorescence or flower number, total yield, fruit weight, or crown number. Higher levels of P did not increased total dry shoot weight, total fresh shoot, weight leaf area, total dry root weight, and leaf number in the present of VAM. However, the cultivars responded differently to VAM inoculation. Vesicular‐ arbuscular mycorrhiza inoculation in combination with P at all levels increased total dry and fresh shoot weight, leaf area, and leaf number compared to application of P alone. The results indicated that it may be possible to increase strawberry stolon production by inoculating the strawberry plants with VAM, a technique which might be useful in nurseries to produce certified strawberry plants.     

Effect of different water supply on plant growth and fruit quality of Lycopersicon esculentum

It is well known from earlier work that water stress and salinity results in depressed plant growth and high fruit quality of tomato (e.g. increased sugar and acid levels), but generally is associated with a low marketable fruit yield. In the present work we investigated whether even a small reduction in water supply (without visible symptoms of water stress) also results in a high fruit quality together with high marketable fruit proportions. To characterize fruit quality sugars (glucose and fructose), titratable acids, odour-active aroma volatiles and vitamin C were investigated. Tomato plants (Lycopersicon esculentum Mill. cv Vanessa) were grown in soil and with the onset of fruit development water supply was varied (70% and 50% water capacity). In the treatment with lower water supply plant growth, and in particular the number of fruit settings were depressed and the sugar and vitamin C concentrations in the fruits were significantly increased, especially during fruit ripening. Furthermore, with lower water supply the concentrations of titratable acids and of C₆ aldehydes (hexanal, (Z)-3-hexenal and (E)-2-hexenal) were significantly increased in the red fruits. Fruit growth was identical in both treatments. The higher levels of sugars, titratable acids, aroma volatiles and vitamin C are responsible for the higher fruit quality under conditions of lower water supply. Since not all fruits of the well watered plants became mature, the marketable yield in both treatments was rather similar and hence, together with a higher fruit quality in the treatment with lower water supply, high proportions of marketable fruits can be harvested.     

番茄地套作蘑菇对土壤肥力和生物活性的影响

通过温室盆栽试验,研究了套作双孢蘑菇(Agaricus bisporus)对潮土速效养分含量、微生物数量、关键酶活性以及番茄(Lycopersicon esculentum Mill.)生长的影响。结果发现,与对照相比,添加双孢蘑菇培养基质对土壤有机碳、矿质氮与速效磷含量均没有显著影响,但速效钾含量显著增加(P<0.05),且土壤细菌数量趋于增加、真菌数量显著减少(P<0.05),土壤脲酶与磷酸酶活性显著增强(P<0.05)、纤维素酶活性显著下降(P<0.05),番茄植株地上部和地下部生物量分别增加130%和67%,但产量没有明显变化;在添加培养基质基础上再接种双孢蘑菇菌种与不接种相比显著降低土壤有机碳含量和脲酶活性(P<0.05),番茄植株生物量也降到与对照相接近的水平,尽管番茄产量没有明显变化,但额外收获了双孢蘑菇。结果表明,套作双孢蘑菇对潮土生物活性具有促进作用,并可以增加单位面积经济产出,因而在潮土地区具有推广潜力。     

Responses of phytohormones and gas exchange to mycorrhizal colonization in trifoliate orange subjected to drought stress

Phytohormones have an essential ability to adapt to abiotic stresses, including drought stress (DS), by mediating physiological and molecular processes. Arbuscular mycorrhizas (AMs) can enhance tolerance of DS, but the information regarding phytohormone changes in AM plants exposed to DS is little known. Trifoliate orange (Poncirus trifoliata) seedlings colonized by an AM fungus Funneliformis mosseae were subjected to DS and well-watered for 6 weeks. Plant growth performance, gas exchange, indole-acetic acid (IAA), gibberellins (GAs), brassinosteroids (BRs), abscisic acid (ABA), methyl jasmonate (MeJA) and zeatin riboside (ZR) were determined. The 6-week DS treatment strongly restricted root mycorrhizal colonization. Mycorrhizal inoculation significantly increased plant growth parameters under DS, as compared with non-mycorrhizal treatment. Mycorrhizal treatment also induced significantly higher leaf-relative water content, net photosynthetic rate, transpiration rate and stomatal conductance but lower intercellular CO₂ concentration and leaf temperature under DS, compared with non-mycorrhizal treatment. Mycorrhizal plants under DS condition represented significantly higher leaf ABA, IAA, GAs, BRs and ZR levels than non-mycorrhizal plants. The study, hence, suggested that mycorrhizal inoculation induced the changes of gas exchange and endogenous phytohormone levels to enhance drought tolerance in trifoliate orange.     

Characterization of Bioactive Compounds in Blueberry and Their Impact on Soil Properties in Response to Plant Biostimulants

ABSTRACT

Vaccinium corymbosum L. (Ericaceae) is a highly valued fruit crop and the most common type of blueberry grown in Chile. Plant growth and yield crop production are affected by agricultural practices and different soil parameters including chemical and biological properties. We performed a field assay to assess the effect of the inoculation with a mixture of 10 microorganisms and the addition of humic substances on the growth of blueberry, quality of fruit and soil chemical and biological properties. Two years after planting, the microbial consortium was more effective than the addition of humic substances recording a 35% increase in shoot dry weight, 70% increase in root dry weight and 104% on total fruits yield compared to the control plants. Total polyphenols and ferric reducing antioxidant power (FRAP) in fruit were increased on humic substance treatment. The combination of both factors increased by 18% organic matter and 60% cation exchange capacity. Soil respiration, microbial biomass C and enzyme activities (dehydrogenase, phosphatase, β-glucosidase, urease, and protease) to a greater extent than individual application. The combined treatment, involving microbial inoculant and humic substances, had an additive effect on improving the biochemical and microbiological quality of the soil.