铁与维生素A水平对小鼠子宫分泌蛋白及胚胎存活率影响

探讨铁与维生素A水平对小鼠子宫分泌蛋白及胚胎存活率影响。选取21日龄昆明种小鼠,随机分为9个处理组,每个处理3个重复,饲喂商品日粮2周,随后采用试验饲粮饲喂。试验采用3×3(铁、维生素A)2因子3水平有重复设计(铁添加水平为3.5、35和175 mg/kg,维生素A水平为400、4000和20000 IU/kg),饲喂第11周时,进行交配,此后选有阴栓的小鼠162只,每个处理3个重复,每个重复6只。结果表明:(1)妊娠3 d,与对照组(Fe1-VA1)相比,5组(Fe1-VA2、Fe1-VA3、Fe2-VA2、Fe2-VA3、Fe3-VA1)添加铁与维生素A对胚胎存活率影响显著(P<0.05),分别提高了20.0%、22.7%、24.7%、19.5%和7.7%。妊娠6 d,添加3.5 mg/kg Fe 4000 IU/kgVA、3.5 mg/kg Fe 20000 IU/kg VA、35 mg/kg Fe 4000 IU/kg VA和35 mg/kg Fe 20000 IU/kg VA效果较好(P<0.05)。妊娠18 d,与对照组相比,添加35 mg/kg Fe 4000 IU/kg VA组显著提高了47.4%(P<0.05);(2)铁与维生素A水平组合在对胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量影响显著(P<0.05)。添加35 mg/kg Fe 4000 IU/kg VA可使胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量和子宫总蛋白分泌含量最高;(3)175 mg/kg铁水平组降低了胚胎存活率、酸性磷酸酶、视黄醇结合蛋白和子宫总分泌蛋白含量。35 mg/kg铁水平组使胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量最高,提高了子宫总分泌蛋白含量;(4)4000 IU/kg维生素A组使胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量最大,提高了总分泌蛋白含量;(5)铁对子宫酸性磷酸酶含量影响显著(P<0.05),维生素A对视黄醇结合蛋白含量影响显著(P<0.05)。适量的铁和维生素A有利于胚胎发育;铁与维生素A水平组合对胚胎存活率、酸性磷酸酶含量、视黄醇结合蛋白含量影响存在互作效应。随着铁在3.5~35.0 mg/kg、维生素A在400~4000 IU/kg范围内增加,子宫铁蛋白、视黄醇结合蛋白增加,更好地促进胚胎存活。     

Superovulationsinduktion bei Merinolandschafen nach einmaliger oder multipler FSH-Applikation

Inhalt: Zyklussynchronisierte Merinolandschafe (n=79) wurden mit FSH-P in einer Gesamtdosis von 25 mg oder 17 mg superovuliert. Während die Versuchsgruppe 1 das Gonadotropin in Form einer mehrfachen Applikation in 12 stündigen Abständen erhielt (Gruppe 1/25 mg und 1/17 mg), wurde die Gesamtdosis bei Versuchsgruppe 2 36 Stunden vor Gestagenentzug (Gruppe 2/25 mg und 2/17 mg) und bei Versuchsgruppe 3 bei Gestagenentzug (Gruppe 3/25 mg und 3/17 mg) verabreicht. Die Embryonengewinnung wurde nach intrauteriner Eksamung der Tiere am 7.-11. Zyklustag durchgefülut.
Die Anzahl der Corpora lutea betrug in den einzelnen Stimulationsgruppen jeweils 11,8 ± 4,6; 9,2 ± 55; 8,4 ± 6,4; 7,2 ± 7,2; 8,8 ± 6,5 und 4,2 ± 2,9 C.l., wobei die Reaktion der Gruppe 3/17 mg signifikant niedriger als die der Gruppe 1/25 mg (p < 0,001), 1/17 mg (p < 0,01), 2/25 mg (p< 0,05) und 3/25 mg (p < 0,05) war. Die anderen Gruppe unterscheiden sich nicht signifikant.
Sowohl die Anzahl der erzielten Ovulationen als auch die Zahl der gewonnenen Eizellen/Embryonen lassen darauf schließen, daß die einmalige FSH-Applikation in höherer Dosierung, insbesondere bei Synchronisationsende, eine sinnvolle Alternative zu den gebräuchlichen Superovulationsmethoden beim Schaf darstellt.     

屡配不孕奶牛血清维生素A、E及铜、锰、锌水平的检测分析

本试验采用原子吸收光谱法和荧光吸收光谱法对14头屡配不孕奶牛和10头正常奶牛血清铜、锰、锌及维生素A、维生素E水平进行检测分析,旨在了解重庆市某奶牛场奶牛屡配不孕与血清中部分微量元素及维生素水平的关系。结果显示,屡配不孕奶牛血清锌、维生素A和维生素E含量与正常奶牛相比差异显著(P0.05),血清铜、锰含量与正常奶牛无明显差异(P0.05)。从检测结果分析推测,血清锌、维生素A和维生素E水平过低可能是造成奶牛屡配不孕的原因之一。     

不同微量元素水平组合对维生素A稳定性的影响

通过采用均匀试验设计,在近似于生产的高温贮藏条件（37℃）下,以4%的仔猪预混料为对象,研究了仔猪预混料中不同水平组合的甲酸亚铁、碱式碳酸锌、碱式碳酸铜、硫酸锰（以下简称为＂新型微量元素组＂）对维生素A稳定性的影响,并在试验中增设2个对照组（不添加铁、铜、锰、锌对照组1和普通硫酸盐对照组2）。贮藏试验期60天。研究表明：（1）预混料中添加微量元素导致了维生素A的额外损失,随微量元素添加量的增加,维生素A的损失率也显著增加（P〈0.05）。（2）添加相同剂量的新型微量元素组对维生素A损失率的影响显著低于普通硫酸盐对照组（P〈0.05）。（3）各处理组中维生素A的最大损失率为56.53%。碱式碳酸铜是预混料贮藏过程中影响维生素A稳定性的主要因素。（4）铁与其它元素的交互作用对维生素A稳定性有破坏作用;高铜和高锌及两者互作极显著（P〈0.01）影响维生素A的稳定性。     

维生素A、E对獭兔繁殖性能的影响

将 2 8只繁殖獭兔随机均分 2组 ,基础日粮相同 ,但试验组在配种前 3天妊娠第 7天于日粮中添加VA8mg/kg、VE10 0mg/kg。结果与对照组相比 :试验组产活仔数提高了 2 0 2 3 %(P <0 0 5 ) ,育成率提高了 6 5 8%(P <0 0 5 ) ,增重速度提高 7 11%(P <0 0 5 )。     

维生素A、E对獭兔繁殖性能的影响

28只繁殖獭兔随机均分两组,基础日粮相同,试验组自配种前3天至妊娠第7天于日粮中添加维生素A8mg/kg、维生素E100mg/kg。结果与对照组相比:试验组产活仔数提高了20.23%(P<0.05),断奶成活率提高了7.23%(P<0.05),增重提高7.11%(P<0.05)。     

Dose-dependent Response to an Intratesticular Injection of Calcium Chloride for Induction of Chemosterilization in Adult Albino Rats

This study concerned the minimum and optimum effective doses of calcium chloride needed for induction of chemosterilization in male albino rats, 30 days after a single intratesticular injection of calcium chloride (CaCl₂.2H₂O) solution at 2.5, 5, 10 or 20 mg per 100 g body weight per testis. There was a significant diminution in the relative wet weight of the sex organs (p<0.01), epididymal sperm count (p<0.001), plasma concentration of testosterone (p<0.01), testicular activities of D,3-hydroxy steroid dehydrogenase (D,3-HSD), 17-hydroxy steroid dehydrogenase (17-HSD) (p<0.01), glutathione S-transferase (GST) (p<0.01), superoxide dismutase (SOD) (p<0.01), and peroxidase (p<0.01), significant elevations in testicular content of malondialdehyde (MDA) and conjugated dienes (p<0.01), along with derangement of seminiferous tubular architecture and degeneration of the Leydig cells in the testis and elevations in the concentrations in the plasma of LH and FSH (p<0.01), commencing at a dose of 5 mg, with the greatest effects at a dose of 20 mg. No significant alterations in these factors occurred at the dose of 2.5 mg in comparison to the control that received only the vehicle. There was no significant alteration in the plasma concentrations of prolactin (p>0.05), corticosterone (p>0.05) or fasting blood glucose or in the rectal temperature (p>0.05) at any of the doses relative to the control group, suggesting that this chemosterilizing procedure did not exert any chronic stress on the experimental animals. From these observations, it may be suggested that 5 mg should be considered as the minimum dose, and 10 mg or 20 mg as the optimum dose, whereas 2.5 mg was ineffective for induction of chemosterilization. There would seem to be little point in using more than 20 mg of calcium chloride for this purpose. Intratesticular injection of calcium chloride at an effective dose may be considered as an alternative to surgical castration.     

泌乳期荷斯坦奶牛维生素A需要量的研究

本试验选择12头荷斯坦泌乳期奶牛,采用3×3拉丁方设计研究了维生素A的需要量以及维生素A对乳成分、抗体、抗氧化性能及血清和牛奶中维生素A浓度等的影响。结果表明：维生素A适宜添加量是1250000IU／d,在此剂量下产奶量最高;在适宜范围内,维生素A剂量的增加,对乳脂、乳蛋白、乳糖和游离脂肪酸的影响不显著（P〉0．05）,而使抗体浓度都有不同程度的增加,但上升不显著（P〉0.05）;SOD和GSH有不同程度的增加,MDA有不同程度的下降,但变化不显著（P〉0.05）;血清和牛奶中的维生素A随之极显著上升（P〈0.01）。     

Vitamin A Deprivation Affects the Progression of the Spermatogenic Wave and Initial Formation of the Blood-testis Barrier,Resulting in Irreversible Testicular Degeneration in Mice

The blood testis-barrier (BTB) is essential for maintaining homeostasis in the seminiferous epithelium. Although many studies have reported that vitamin A (VA) is required for the maintenance of spermatogenesis, the relationships between the BTB, spermatogenesis and VA have not been elucidated. In this study, we analyzed BTB assembly and spermatogenesis in the testes of mice fed the VA-deficient (VAD) diet from the prepubertal period to adulthood. During the prepubertal period, no changes were observed in the initiation and progression of the first spermatogenic wave in mice fed the VAD diet. However, the numbers of preleptotene/leptotene spermatocytes derived from the second spermatogenic wave onwards were decreased, and initial BTB formation was also delayed, as evidenced by the decreased expression of mRNAs encoding BTB components and VA signaling molecules. From 60 days postpartum, mice fed the VAD diet exhibited apoptosis of germ cells, arrest of meiosis, disruption of the BTB, and dramatically decreased testis size. Furthermore, vacuolization and calcification were observed in the seminiferous epithelium of adult mice fed the VAD diet. Re-initiation of spermatogenesis by VA replenishment in adult mice fed the VAD diet rescued BTB assembly after when the second spermatogenic wave initiated from the arrested spermatogonia reached the preleptotene/leptotene spermatocytes. These results suggested that BTB integrity was regulated by VA metabolism with meiotic progression and that the impermeable BTB was required for persistent spermatogenesis rather than meiotic initiation. In conclusion, consumption of the VAD diet led to critical defects in spermatogenesis progression and altered the dynamics of BTB assembly.     

不同维生素A添加量对肉用仔鸡的生长发育及血浆、肝脏中维生素E含量的影响

以Abor Acres商品代肉用仔鸡为对象,进行了两次不同维生素A水平饲养效果试验。第一次试验用1,600只一日龄混合雏,分五个维生素A添加量。结果表明:每千克饲粮添加750 IU微生素A醋酸酯的鸡,49日龄增重显著低于12,000IU组;添加1500 IU、4000 IU、8000 IU三个中间水平鸡的增重与750和12000 IU没有显著差异。第二次试验用576只一日龄混合雏,检测了添加20,000 IU和1,500 IU两种维生素A水平的效果,结果发现每千克饲粮添加20,000IU维生素A醋酸酯的增重显著低于1,500IU组。高水平组鸡的血浆、肝脏维生索E含量分别比低水平组低6和10倍。     

维生素A、E和叶酸对母猪繁殖性能的调控

维生素A、E和叶酸对母猪的繁殖性能具有重要的影响.该综述主要对维生素A、E和叶酸的添加阶段、添加方式、添加剂量及其添加形式进行分析比较,总结得出维生素的最佳使用方式,为母猪生产提供参考.     

不同剂型剂量维生素A对家禽免疫功能的影响及其机制的研究

实验采用2×3因子设计,选用一日龄艾维因肉仔鸡288只,分6个处理,下设4个重复,各重复含12只鸡,对2,700IU/kg、20,000IU/kg 两个剂量和粉剂、进口乳剂、自制乳剂三个剂型的维生素 A(V.A)对家禽免疫功能的影响及其机制进行了研究。结果发现:1.高剂量 V.A 有使胸腺、脾脏、法氏囊等免疫器官的绝对重量增加的趋势,但统计差异不显著(P>0.05)。2.高剂量 V.A 有提高血清抗体滴度的趋势,但统计差异不显著(P>0.05),而乳剂处理极显著优于粉剂(P<0.01)。3.高剂量 V.A 有提高血浆淋巴 T 细胞百分比的趋势,但差异不显著(P>0.05);在绝对值上,乳剂优于粉剂,而统计上无差异(P>0.05)。4.大剂量添加 V.A 显著提高血浆中 CAMP(环——磷酸腺苷)的浓度(P<0.05),但乳剂处理的血浆 CAMP 含量显著低于粉剂处理的相应值(P<0.05)。5.V.A 的剂型剂量二因素对体增重、饲料效率、死亡率等无显著影响(P>0.05),但大量添加 V.A 极显著地提高鸡只肝中 V.A 的浓度(P<0.01)。6.V.A 对鸡只免疫功能的影响与血浆中 CAMP 有关。     

犊牛维生素A缺乏造成骨骼发育异常的探讨研究

一牛场出现犊牛腿部骨骼发育异常、多数母牛胎衣不下的情况,开展现场临床检查,实验室检测犊牛和母牛的血清Ca、P、碱性磷酸酶,犊牛骨碱性磷酸酶,母牛和犊牛血清维生素A含量。结果表明,部分犊牛Ca、P、碱性磷酸酶偏低,其他犊牛以上指标正常,所有检测的犊牛、母牛血清维生素A含量均远低于参考值,处于严重缺乏状态。通过临床检查、实验室检验及药物试验,证明该牛场此次犊牛腿部骨骼发育异常及母牛胎衣不下是以维生素A缺乏为主因造成的。     

乳酸菌制剂在黑毛和牛育肥中的使用效果

[目的]旨在通过黑毛和牛（Japanese Blackcattle）育肥后期投服乳酸菌制剂Entero—coccus faecalis FK-23粉末,探讨付与牛肉的附加价值和该制剂的实用性。[方法]供试牛10头,随机分为试验组和对照组,试验组5头,每头每日投服乳酸菌制剂8．0g。测量体重（BW）,静脉采血检测白细胞数（WBC）,维生素A（VA）,维生素E（VE）．总胆固醇（TC）,谷草转氨酶（GOT）,血尿素氮（BUN）及血糖（GLC）浓度;HPLC测验中性脂肪的脂肪酸成分。[结果]试验组与对照组比较。食欲亢进,日增重（DG）明显增加（P〈0．05）,血清VE和TC明显升高（P〈0．05）,VA维持预计水平走向不变。中性脂肪的不饱和脂肪酸比例出现增高趋势,出栏体重增长4．3％,胴体重增加9．3kg。[结论]结果提示,黑毛和牛育肥后期投服乳酸菌E．faecalisFK-23制剂可以获得VE含量较高的高附加价值牛肉并能提高其生产性能。     

维生素A对0～3周龄北京鸭生产性能和肠道发育的影响

本研究旨在评价日粮中添加维生素A对0～3周龄肉鸭生产性能、血清中维生素A含量和肠道发育的影响.选择1日龄健康北京鸭360只,随机分为5个组,每组6个重复,每个重复12只鸭,分别饲喂在基础日粮中添加0.2500、5000、10000、15000 IU/kg维生素A的试验日粮,试验期为3周.结果表明:日粮中维生素A水平为2 500 IU/kg即能满足北京鸭基本需要;维生素A添加水平在0～10000 IU/kg时,血清中维生素A含量随着日粮中维生素A添加水平的增加而极显著提高(P<0.01),10 000和15 000 IU/kg添加组之间差异不显著(P>0.05);2500 IU/kg组的绒毛长度、十二指肠隐窝深度和肠壁厚度均显著或极显著高于0 IU/kg组(P<0.05或P<0.01),但与其他组差异不显著(P>0.05).由此可见,饲料中适量增加维生素A的添加水平可提高北京鸭生产性能,增加肠道的绒毛长度;肠道绒毛的发育状况和血清中维生素A均可以作为评价肉鸭维生素A营养状况的指标.     

实验性肉雏维生素A缺乏对免疫器官影响的形态学研究

150只一日龄罗曼混合肉雏被随机分为四组,分别饲喂含维生素A为0、750、3000和6000IU的日粮。在20、30和45日龄时,每组剖检5只鸡,分离并称重免疫器官后,取材,石蜡包埋,切片,镜检。结果表明:维生素A缺乏和不足使免疫器官组织萎缩,间质结缔组织增生,上皮角化,腔上囊和胸腺的相对重量明显减少(P<0.01)。对免疫器官的损害程度是腔上囊>胸腺>脾脏>盲肠扁桃体。提高日粮中维生素A含量有提高肉雏增重、饲料报酬和免疫器官相对重量之趋势。     

Dynamik der LH- und Testosteron-Sekretion bei Beagle-Rüden verschiedener Altersgruppen*, **

Inhalt: Bei 1 7 klinisch geschlechtsgesunden Beagle-Rüden verschiedener Altersgruppen (1: 7 bis 8 Monate; II: 12 Monate; III: 23 Monate;IV: 3 bis 4 Jahre; V: 8 Jahre) wurden die Körper- und Hodenmaβe sowie die Sekretionsmuster von LH und Testosteron im peripheren Blutplasma bestimmt. Von jedem Ruden wurden im Abstand von 10 bis 14 Tagen zwei Blutprobenserien à 25 Einzelproben über einen Zeitraum von jeweils sechs Stunden unter Einhaltung 15 minütiger Intervalle gewonnen. Die Hodenlange war bei den 7 bis 8 Monate alten Tieren signifikant kleiner (p ≤ 0,01) als bei den älteren Rüden. Die 8 Jahre alten Hunde hatten die gröβten Gonaden. LH und Testosteron zeigten ein ausgeprägt pulsatiles Sekretionsmuster, wobei die LH-Werte zwischen 1,2 und 96 ng/ml und Testosteron zwischen 0,08 und 16 ng/ml varüerten. Die Pulsfrequenz unterschied sich nicht signifikant zwischen den Gruppen und lag im Mittel bei 4,5 Pulsen 16 Stunden. LH-Pulse wurden gewöhnlich von Testosteron-Pulsen gefolgt; der mittlere zeitliche Abstand zwischen beiden Ereignissen betrug 37 ± 15 Minuten. Die Plasmaproben der Gruppe I enthielten die höchsten LH- und die geringsten mittleren Testosteronkonzentrationen (15, 7 ± 13,6 ng/ml bzw. 2,0 ± 1,2 ng/ml). Das höchste durchschnittliche Testosteronniveau (3,6 ± 1,9 ng/ml) war in Gruppe III zu beobachten. Bei den 8 Jahre alten Hunden lag die mittlere LH-Konzentration signifikant unter derjenigen der 7 bis 8 und der 21 bis 23 Monate alten Tiere (p ± 0,01 bzw. p ± 0,05). Contents: Dynamics of LH and testosterone secretion in male beagles of different age In 17 clinically healthy male beagles of five age groups (I: 7 to 8 months, II: 12 months, III: 23 months, IV: 3 to 4 years, V: 8 years) the body size and the testicular dimensions were measured and the concentrations of LH and testosterone were determined in the peripheral blood. Two series of blood samples were collected from each dog with an interval of 10 to 14 days, each series consisting of 25 samples, taken every 15 minutes for 6 hours. The testicular length was significantly smaller (p ± 0.01) in the 7 to 8 months old dogs than in the older animals. The 8 year old dogs had the largest gonads. LH and testosterone secretion showed a marked pulsatility, with LH values ranging from 1.2 to 96 ng/ml and testosterone ranging from 0.08 to 16 ng/ml. Pulse frequency did not differ between groups and averaged 4,5 pulses/6 hours. LH pulses usually preceded testosterone pulses by an average of 37 ± 15 minutes. The samples of group I showed the highest mean LH concentration and the lowest mean testosterone value (15.7 ± 13.6 ng/ml and 2.0 ± 1.2 ng/ml resp.). The highest mean testosterone level (3.6 ± 1.9 ng/ml) was observed in group III. In the 8 year old dogs the mean LH-concentration was significantly lower than that of the animals aged 7 to 8 and 21 to 23 months (p ± 0.01 and p ± 0.05 resp.).     

Utilization of Dietary β-Carotene and Retinol Acetate by Goslings and Young Geese

One-day-old and 3-month-old geese were fed different concentrations of g -carotene (5, 10, 50 or 150 mg kg -1 feed) or retinol acetate (10 000, 20 000 or 40 000 IU kg -1 feed) for about 3 weeks to assess their ability to convert g -carotene to vitamin A. Blood serum concentrations of g -carotene, retinol, f -tocopherol, triacylglycerides and cholesterol, as well as liver concentrations of g -carotene, retinol, triacylglycerides and cholesterol, were included as response factors. g Carotene was not detectable in serum and liver. Serum and liver retinol concentrations were positively correlated with dietary concentrations of g -carotene and retinol acetate ( R 2 = 0.84 and 0.95, respectively). In goslings 1 mg of g -carotene corresponded to 63 IU retinol and in young geese to 1216 IU, equivalent to 3.8% and 72.9%, respectively, of the theoretical value of 1667 IU retinol. Cholesterol concentrations in blood serum and liver were not affected ( P > 0.05) by the dietary levels of g -carotene and retinol acetate. The concentration of f -tocopherol in blood serum decreased with increasing dietary levels of g -carotene and retinol acetate. In the goslings the concentration of triacylglycerides in serum and liver was significantly ( P < 0.05) influenced by the intake of g -carotene, but not by the intake of retinol acetate. In the young geese the serum concentration of triacylglycerides decreased ( P < 0.05) with increasing dietary levels of g -carotene, but was not affected by the dietary concentrations of retinol acetate. The concentration of triacylglycerides in liver tissue of young geese was not affected by the dietary concentration of either g -carotene or retinol acetate ( P > 0.05).     

Effect of controlled alterations in maternal dietary retinol on foetal and neonatal retinol status and pregnancy outcome in pigs

The objective of this experiment was to examine the effects of feeding a vitamin A deficient diet (VAF) to pigs at different times on day 100 of foetal and days 0, 1 and 2 of neonatal development. Three treatments included a control (n = 12), VAF for 100 days before mating and during the first month of pregnancy (n = 13; VAF–control), and VAF during the oestrous cycle before mating and throughout pregnancy (n = 13; control–VAF). On day 100 of pregnancy, maternal liver and plasma retinol concentrations were reduced in both groups of gilts fed a vitamin A free diet compared to controls (P < 0.001 and P < 0.01, respectively). Day 100 foetal liver retinol concentrations were not affected by dietary treatment, whereas foetal plasma concentrations were higher in foetuses carried by gilts fed the VAF–control diet (P < 0.05). Piglets born to mothers fed the control–VAF, but not the VAF–control diet had consistently lower hepatic and plasma liver retinol concentrations (P < 0.05). Moderate reductions in maternal vitamin A at either stage of pregnancy did not affect pregnancy rate, litter size, progesterone secretion or the allometric relationships between foetal or neonatal organ and total body size. Reduced vitamin A during conception and early pregnancy, but not during later pregnancy, was associated with increased within-litter uniformity in birth weight (P < 0.05) and a tendency for fewer low birth weight piglets, but this needs to be confirmed in a greater number of sows. The mechanism underlying this effect is not known, but appears to not involve an alteration in progesterone production.     

Vitamin D ameliorates liver pathology in mice caused by exposure to endocrine disruptor bisphenol A

Background:Increasing evidence suggests that bisphenol A (BPA) induces liver pathological changes. Further, an association between BPA and circulating vitamin D (VitD) levels were documented.Aim:The role of VitD in BPA-induced liver pathological changes was explored in this study.Methods:Healthy 4.5-week-old male (n = 35) and female (n = 35) Swiss albino mice were used in this study. The animals were randomly divided into control and treated groups. The control groups were further divided into sham (no treatment) and vehicle (corn oil), whereas the treated groups were also divided into VitD (2195 U/kg), BPA (50 μg/kg), and BPA + VitD (50 μg/kg + 2195 U/kg) groups. For 6 weeks (twice a week), the animals were dosed intraperitoneally. One week later (at 10.5-weeks-old), the animals were sacrificed for biochemical and histological analyses.Results:BPA produced a considerable rise in the body and liver weights in both genders of mice when compared to control mice. BPA also caused significant increases in the liver damage markers alanine transaminase (ALT), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT). It also induced liver histopathological changes, including higher apoptotic indices in both genders. On the other hand, treatment with VitD considerably reduced liver damage and slightly decreased the apoptotic index rate. The ALP, ALT, and GGT levels were also markedly reduced. VitD has been proven to have a protective effect on both genders.Conclusions:According to our findings, VitD protects mice from BPA-induced liver damage, possibly via suppressing liver damage markers.