Shortened Temperature Program for Application with a Rapid Visco Analyser in Prediction of Noodle Quality in Wheat

The use of the Rapid Visco Analyser (RVA) for application in the screening of wheat breeding lines for starch quality and potential noodle quality has been limited by relatively low sample throughput. Current methods generally enable only 20–30 samples to be tested each day. This study sought to develop a more rapid time‐temperature profile that could be applied to whole meal samples. A profile that involved a total analysis time of 7.5 min/sample gave measurements of peak viscosity (PV) and breakdown (BD) on whole meal that were highly correlated with corresponding measurements obtained using a more conventional profile that had been applied to low‐extraction flours. BD and PV were also highly correlated with the total texture score of ramen (Chinese‐style alkaline noodles as manufactured in Japan), but only when 1 mM AgNO₃ was used to eliminate the effects of α‐amylase.     

Genotypic and Environmental Modification of Asian Noodle Quality of Hard Winter Wheats

The relative effects of environment, genotype, and their interactions on the modification of Asian noodle quality attributes were assessed using 38 winter wheat (Triticum aestivum L.) cultivars and breeding lines grown in replicated trials at three Nebraska locations in harvest year 2000. Noodle color was determined in both white salted and yellow alkaline procedures, and noodle textural features were investigated by producing white salted noodles. Significant environmental, genotypic, and genotype‐by‐environment variation was observed for nearly all initial and 24‐hr noodle color traits in both types of noodles. Significant genotypic effects were observed for several textural traits, while significant environmental effects were observed only for noodle hardness and water uptake. However, among the noodle textural traits, the genotype‐by‐environment interaction was significant only for noodle firmness. High and significant phenotypic correlations were observed between color traits in the two noodle applications. Genetic correlations were of lower magnitude, indicating the possibility of breeding wheats specifically for various noodle color types. Strong negative phenotypic and genetic correlations were observed between flour protein content and noodle brightness (L*) values in both yellow alkaline and white‐salted applications. Textural traits largely were independent of noodle color traits. When significant phenotypic or genetic correlations were observed between variable pairs, invariably similar correlations were observed with flour protein content. Noodle cutting force, cutting area, and final thickness showed strong phenotypic and genetic correlations with each other and with protein content. These variables largely were independent of noodle firmness and hardness, which were, in turn, more dependent on alleles at the wheat wx‐A1 and wx‐B1 (waxy) loci. Noodle firmness was greatest in flours from wild‐type wheats; lines with a null allele only at the wx‐A1 locus did not differ from wild‐type. Softest noodles were produced from lines carrying null alleles at both wx‐A1 and wx‐B1, while lines with a null only at wx‐B1 were intermediate in softness.     

Effects on Pasting Viscosity of Starch and Flour from Different Operating Conditions for the Rapid Visco Analyser

Three wheat flours, three wheat starches, a regular maize starch and a waxy maize starch were subjected to a number of different RVA profiles. Five different initial temperatures were used, 40, 50, 55, 60, and 65°C, with different initial holding times (0–3 min), heating times (2fl–10 min), holding times at 95°C (0–6 min), cooling times (2–6 min), and final hold times (0–10 min) being applied. A range of final temperatures of 30–60°C was also utilized. Significant variations in viscosity were observed with these conditions, particularly in wheat starch and flour. The most important parameters causing these variations were the initial temperature, the heating rate, and the final holding time. Short initial holding times also resulted in a wider spread of values for peak viscosity although there was little effect on the mean value and no significant effect on the holding strength or final viscosity. The final temperature was also important in that lower temperatures gave more viscous gels. Provided that the desired cooling rate could be achieved, varying the cooling time had no effect on the peak or trough viscosities and only a very minor effect on the final viscosity. If final temperatures of 40°C or lower are to be used, the cooling conditions and final hold time would need to be adjusted so that maximum viscosity could be achieved. A proposal for a standard Rapid Visco Analyser (RVA) procedure is: at least 1 min at 50°C, heat to 95°C over 4 min, hold at 95°C for 4 min, cool to 50°C in 3 min, and hold at 50°C for 4 min. These conditions should minimize variation within samples and should allow a better comparison between samples.     

Survey of White Salted Noodle Quality Characteristics in Wheat Landraces

The introduction of novel quality characteristics from wheat (Triticum aestivum L.) landraces can enhance the genetic diversity of current wheat breeding programs. The composition of starch and protein in wheat is important when determining the end‐product quality, particularly for white salted noodles (WSN). Quality characteristics that contribute to the production of improved WSN include high starch pasting peak viscosity, low amylose content, high proportion of A‐type granules, low protein content, soft grain texture, and high protein quality as measured by SDS sedimentation volume. A survey of 133 wheat landraces from Afghanistan, China, Egypt, Ethiopia, India, Iran, Syria, and Turkey was conducted to examine the genetic variability of starch and protein quality characteristics. Two wheat cultivars, Rosella and Meering, were used as the quality controls. The variation in starch pasting peak viscosities observed among the wheat landraces had a range of 175–295 Rapid Visco Analyser units (RVU), where 52 of the landraces were not significantly different from Rosella, a commercial soft grain wheat with high pasting properties. The amylose content of the landrace population was 23.4–30.2%, where 17 landraces had significantly lower values than Rosella. The proportion of A‐type granules was 60.5–73.9%, where 112 landraces had significantly higher values than Rosella. The grain texture hardness score was 28.0–99.3, the total protein content was 8.0–15.1%, and the adjusted SDS sedimentation volume (SDS/protein) was 1.6–7.0 mL/%P. The landrace AUS4635 had high starch pasting peak viscosity, high breakdown, low amylose content, low protein content, soft grain texture, and high protein quality flour. This wheat is an ideal parent to use in a breeding program that increases the genetic variation available to develop cultivars with high‐quality WSN characteristics.     

Milling Quality and White Salt Noodle Color of Chinese Winter Wheat Cultivars

Improvement of milling quality is an important aspect in wheat breeding programs. However, the milling quality of Chinese wheats remains largely unexplored. Fifty‐seven Chinese winter wheat cultivars from four regions were used to investigate the variation of milling quality parameters and to determine the associations between milling quality traits and color of noodle sheet. Substantial variation was presented for all measured parameters in this germplasm pool. Complete soft, hard, and medium‐hard types were observed. Soft wheat and hard wheat show significant differences in flour ash content, flour bran area, and flour color grade. No simple trait can be used to select for flour milling quality. High flour ash content and bran speck area contributed negatively to brightness of dry flour. Correlation coefficients (r) between L* value of dry flour and flour ash content and bran speck area were ‐0.47 and ‐0.65 for hard cultivars, and ‐0.51 and ‐0.72 for soft cultivars, respectively. Flour color grade (FCG) was significantly and positively associated with bran speck area; r = 0.56 and 0.73 for hard and soft wheats, respectively. There was a high correlation between FCG and L* value of flour water slurry (r = ‐0.95). Strong associations were also established between milling quality index (MQI) and FCG, L* value of dry flour, flour‐water slurry, and white salted noodle sheet for both hard and soft wheats. In conclusion, substantial progress could be achieved in improvement of milling quality in Chinese winter wheats through genetic selection, and FCG and MQI could be two important parameters for evaluation of milling quality in breeding programs.     

Relationship Between Protein Characteristics and Instant Noodle Making Quality of Wheat Flour

We investigated the relationship between the protein content and quality of wheat flours and characteristics of noodle dough and instant noodles using 14 hard and soft wheat flours with various protein contents and three commercial flours for making noodles. Protein content of wheat flours exhibited negative relationships with the optimum water absorption of noodle dough and lightness (L*) of the instant noodle dough sheet. Protein quality, as determined by SDS sedimentation volume and proportion of alcohol‐ and salt‐soluble protein of flour, also influenced optimum water absorption and yellow‐blueness (b*) of the noodle dough sheet. Wheat flours with high protein content (>13.6%) produced instant noodles with lower fat absorption, higher L*, lower b*, and firmer and more elastic texture than wheat flours with low protein content (<12.2%). L* and free lipid content of instant noodles were >76.8 and <20.8% in hard wheat flours of high SDS sedimentation volume (>36 mL) and low proportion of salt‐soluble protein (<12.5%), and <75.7 and >21.5% in soft wheat flours with low SDS sedimentation volume (<35 mL) and a high proportion of salt‐soluble protein (>15.0%). L* of instant noodles positively correlated with SDS sedimentation volume and negatively correlated with proportion of alcohol‐ and salt‐soluble protein of flour. These protein quality parameters also exhibited a significant relationship with b* of instant noodles. SDS sedimentation volume and proportion of salt‐soluble protein of flours also exhibited a significant relationship with free lipid content of instant noodles (P < 0.01 and P < 0.001, respectively). Protein quality parameters of wheat flour, as well as protein content, showed significant relationship with texture properties of cooked instant noodles.     

黏粒阳离子交换量估测模型的优化研究

为了优化土壤黏粒阳离子交换量估测模型,提升估测结果准确性,为土壤系统分类检索提供可靠数据支持,以江西省土壤为主要研究对象,根据有机质、粉粒阳离子交换量对现有估测模型的影响,将有机质含量低于6 g·kg–1的土壤样本定为低有机质样本,高于6 g·kg–1的土壤定为高有机质样本开展分类建模.高有机质样本中主要误差因子为有机...     

Effects of Protein Content and Composition on White Noodle Making Quality: Color

Wheat cultivars, representing three winter and three spring wheats were grown in western Canada with six levels of nitrogen fertilizer and flours were prepared from them with an extraction rate of 65%. Using a chromameter, flour color and the color of uncooked white noodle sheets made from these flours with different resting times were assessed. The cooked noodle sheet color was also assessed. While protein content initially declined with added nitrogen and increased with further nitrogen addition, brightness (L*) of flour decreased and redness (a*) and yellowness (b*) increased. Positive correlation coefficients of flour brightness with particle size index (PSI) were also observed. Flour redness (a*) and yellowness (b*) were also affected by flour moisture content, whereas L* values were not significantly correlated with moisture contents. For the uncooked white noodle sheet, as protein content increased brightness decreased but there was an increase in a* and b* values. Thus, the L* value for noodle sheets was negatively correlated with the a* and b* values. The percentages of monomeric protein and soluble glutenin in flour were equal to or better than protein content in relation to most noodle sheet color characters. Uncooked noodle sheet brightness decreased, while redness and yellowness increased with rest time. In general, uncooked white noodle sheets prepared from different wheat flours can be ranked in terms of brightness and yellowness within each level of nitrogen fertilization.     

Effect of Amaranthus and Buckwheat Proteins on Wheat Dough Properties and Noodle Quality

Isoelectric protein concentrates (IPC) were prepared from one buckwheat (Fagopyrum esculentum) and five Amaranthus genotypes. Their effect on the mixing properties of a wheat flour was studied. Mixograph and dynamic oscillatory measurements showed significant increases in dough strength with the addition of 2 and 4% IPC, correlated to the water-insoluble fraction level of the IPC. The same IPCs were used at 2% level to supplement a wheat flour in making Chinese dry noodles. Measurable changes in both the raw and cooked noodle color were observed, and the change caused by addition of buckwheat IPC was substantial. Some of the IPCs caused an increase in cooking loss and only one caused an increase in weight, while increase in volume of the cooked noodles was not significantly affected. The changes in the rheological properties of cooked noodles due to addition of IPCs were measured. Overall, their effects were favorable, but the changes were statistically significant in only a few cases. The substantial dough-strengthening effect of the IPCs was hence not effectively translated into improved cooked noodle quality, and possible reasons for this are discussed.     

优质新疆拉面品种品质评价的多重PCR体系构建与应用

新疆拉面是深受人们喜爱的特色面食之一,建立优质新疆拉面品质评价体系对其专用品种选育和评价具有重要意义。本研究构建了3套检测新疆拉面优质基因的多重PCR体系,并对46份新疆冬小麦(Triticum aestivum L.)进行了检测。体系Ⅰ可同时直接检测Ax2、Pinb-D1b和ω-secalin(1B·1R易位)3种基因,体系Ⅱ可同时直接检测两个位点Psy-A1(Psy-A1a、Psy-A1b、Psy-A1c)和Ppo-D1(Ppo-D1a、Ppo-D1b)的5种基因,体系Ⅲ可同时直接检测Glu-B3a、Wx-B1a和Wx-B1b3种基因,3套体系检测对照品种(系)的结果均与已知基因型完全一致。对46份新疆冬小麦的检测结果表明,Ax2、Pinb-D1b和不含ω-secalin(非1B·1R易位)基因出现的频率依次为30.4%、45.7%和78.3%,Psy-A1a、Psy-A1b、Psy-A1c、Ppo-D1a和Ppo-D1b基因的频率分别为94.5%、4.3%、2.2%、91.3%和8.7%,Glu-B3a、Wx-B1a和Wx-B1b基因的频率依次为21.7%、91.3%和8.7%;其中,与新疆拉面优质性状相关的5个基因Ppo-D1a、不含ω-secalin、Pinb-D1b、Glu-B3a和Ax2的频率明显高于全国平均水平;共检测到19种优质拉面基因的组合类型,其中含1、2、3、4和5个优质基因的组合类型依次占参试品种(系)的13.0%、32.6%、26.1%、23.9%和4.3%,没有发现同时含6或7个优质基因的品种(系),出现频率最高的优质基因组合类型为Ppo-D1a/不含ω-secalin(非1B·1R易位)、Ppo-D1a/Glu-B3a/不含ω-secalin和Ppo-D1a/Ax2/Pinb-D1b/不含ω-secalin。研究结果表明,本研究建立的3套多重PCR体系,结果稳定可靠,可作为新疆拉面品种品质性状快速评价的方法;利用本研究构建的多重PCR体系和筛选出的含优质基因(组合)的品种(系),开展小麦品质评价和分子聚合育种,将会提高新疆拉面品种评价和选育的效率。     

Impacts of SSIIa‐A Null Allele on Durum Wheat Noodle Quality

A small increase in amylose content may impact end‐product quality of wheat. The effect of elevated amylose content in durum wheat is not known. We surveyed 255 durum wheat accessions and found two genotypes that lacked the SGP‐A1 protein. These genotypes were crossed to Mountrail, an adapted durum genotype, to create populations segregating for the SSIIa‐Ab null allele. Our goal was to determine the influence of allelic variation at the SSIIa‐A locus on semolina properties and end‐product quality with noodles as a test product. Amylose content increased 3% and cooked noodle firmness increased 2.8 g·cm for the SSIIa‐Ab class compared with the SSIIa‐Aa class for the PI 330546 source, but no change in either trait was detected between classes for the IG 86304 source. The SSIIa‐Ab class had a 10% reduction in flour swelling compared with the SSIIa‐Aa class for both crosses. Grain protein and semolina yield did not differ between SSIIa‐A classes. The relationship between flour swelling power and noodle firmness did not differ between SSIIa‐A allelic classes within a cross. The different results for amylose content and noodle firmness between these sources may be because the two sources of the SSIIa‐Ab null mutation contributed different linkages to the segregating populations. Results show that the SSIIa‐Ab allele could be used to produce durum‐based products that are slightly more firm in texture. However, the effect of the SSIIa‐Ab allele may depend on the source.     

Use of Mixolab in Predicting Rice Quality

Mixolab is a new instrument with capability to measure starch pasting properties on actual dough. It characterizes dough rheological behavior using a dual constraints of mixing and temperature. Rice samples (183) collected from 15 provinces across China were tested to determine the possibility of using Mixolab in predicting rice quality. Mixolab measurements, torque (Nm) at different mixing and heating stages (C1 to C5) were compared with rice quality characteristics (gelatinization temperature and consistency, amylose and protein contents), Rapid Visco‐Analyser (RVA) parameters and sensory assessments scores of cooked rice. Our results showed that Mixolab parameters were good indicators of amylose and protein content and quality suggested by significant correlations among Mixolab parameters, and between Mixolab and RVA measurements. Based on a subsample of 30 rice cultivars, correlation coefficients between the Mixolab parameter C4 and sensory assessment characteristics of palatability and total sensory score was negatively significant (P < 0.05). C_b (C3 – C4) was also significantly correlated with flavor (P < 0.05). The rice samples that gave high palatability and total sensory scores had low C4 values and low amylose contents. The cooked rice with high flavor had high values of C_b and GT but low protein content. It is possible to determine physicochemical properties of rice flour and sensory characteristics of cooked rice using Mixolab parameters.     

美拉德反应制备鸡肉风味基料的工艺条件优化

为探究美拉德反应条件对鸡肉风味调味料品质的影响,本试验以鸡胸肉蛋白酶解液为研究对象,采用模糊数学感官评价和气相色谱-离子迁移谱联用技术(GC-IMS)研究还原糖添加量、反应温度、反应时间和pH值4个热反应条件对鸡肉风味基料感官品质和挥发性有机物(VOCs)的影响。结果表明,添加3%木糖的鸡胸肉蛋白酶解液(pH值7),在101.1℃下反应86 min可获得一种感官性状好、风味佳的鸡肉风味基料,且该制品VOCs丰度高,与市售罐头鸡汤风味相似度较高。本研究为低值鸡肉制品的深度开发提供了一定的理论参考。     

Relationship Between Physicochemical Properties of Starches and White Salted Noodle Quality in Japanese Wheat Flours

This study describes the effect of starch properties of Japanese wheat flours on the quality of white salted noodles (WSN). Starch was isolated from 24 flours of 17 Japanese cultivars and amylose content was determined along with pasting properties by Rapid Visco Analyser (RVA), thermal properties by differential scanning calorimetry (DSC), and the distribution of amylopectin chain length by high‐performance anion exchange chromatography (HPAEC). Twenty flours were used to prepare WSN. As expected, 5–6% lower amylose content was associated with good WSN quality (higher scores in softness, elasticity, and smoothness). RVA analysis indicated that the pasting temperature had the greatest influence on WSN quality, while breakdown and setback showed slight effects on WSN quality. DSC results showed that lower endothermal enthalpy (ΔH) in the amylose‐lipid complex was associated with good WSN quality. Chainlength distribution of amylopectin by HPAEC was not an important factor in relation to WSN quality.     

Physicochemical Properties of Pueraria Root Starches and Their Effect on the Improvement of Buckwheat Noodle Quality

The physicochemical properties of starches from cultivated Pueraria thomsonii Benth were examined and compared with those of P. lobata (Willd.) Ohwi and other root starches, and the effect of pueraria root starches on the improvement of buckwheat noodle quality was investigated. The total content of isoflavones in P. thomsonii root starches was higher than in P. lobata root starches, and the size and uniformity of those particles displayed a significant difference. The gel stabilities of pueraria root starches were similar and more favorable than those of potato starch and sweet potato starch. For the amylose molecular properties of pueraria root starches, the λ_max and blue value index were higher than those of the potato starch and the sweet potato starch, whereas the amylose content and degree of polymerization were much lower in comparison. However, amylopectin branch lengths of pueraria root starches were shorter. Thus, pueraria root starches could improve the quality of buckwheat noodles and enhance their nutritional function. Therefore, pueraria root starches may be regarded as raw materials that influence the quality of buckwheat noodles.     

保加利亚尖椒再生及遗传转化条件的优化

为了提高辣椒子叶不定芽的伸长率和遗传转化效率,本研究以保加利亚尖椒子叶为外植体,通过正交试验分别对影响保加利亚尖椒子叶不定芽伸长的激素组合以及遗传转化参数进行了优化。结果表明：诱导不定芽伸长的最佳激素组合为0.2mg/LIAA＋1.0mg/LGA3＋0.1mg/LPBU,不定芽伸长率最高为60%;以5mg/L潮霉素为选择压,预培养时间为4d、共培养时间为2d、侵染时间为20min时,诱导的抗性不定芽比率最高。本研究建立的辣椒再生及遗传转化体系为辣椒转基因研究奠定一定的基础。     

不同品种甘薯淀粉组分、物化及粉条品质的比较研究

为探讨不同品种甘薯淀粉特性与其粉条品质的关系,本研究选用济薯25、济薯26以及商薯19共3个品种于丘陵山地进行试验,收获后进行块根干物质含量、产量测定以及淀粉分离,分析淀粉组分、老化和糊化特性,并对粉条品质进行测定.结果表明,济薯25的薯千产量显著高于其他2个品种(P＜0.05),且3个品种的淀粉结晶结构、形态相似.3...     

Zinc Fertilizer Test Kit for Semi-Quantitative Verification of Fertilizer Quality

Zinc (Zn) addition is often recommended for rice culture, but the quality of Zn fertilizer is variable. A semi-quantitative test kit was developed from a zincon colorimetric method to provide buyers an estimate of Zn content in zinc sulfate or zinc oxide fertilizers. The kit includes tools for sample dilution, capsules with sufficient zincon and borax buffer reagent for color development of one sample, and a five-panel color chart to categorize the sample at 0, 5, 10, 15, or 20% Zn. An analysis of user errors indicated that water impurity would cause the most significant problem, resulting in errors of one color category or more. The errors associated with the semi-quantitative measurements of fertilizer and water using tools provided in the kit did not cause significant problems affecting the final color category of the fertilizer, making the kit an acceptable method of estimating Zn content of granular fertilizers.     

安兴村的优化梯田配套增产措施

安兴村的优化梯田配套增产措施高维明（甘肃省庆阳地区水保站甘肃西峰７４５０００）正宁县水和乡安兴村共有８个村民小组，４６０户，农业人口２２０３人，农业劳动力１２０７个；总耕地面积３４８．７ｈｍ２，其中山地１５７．８ｈｍ２，占总耕地面积的４５．２％；人均...     

肖咀乡优化梯田建设成效与经验

肖咀乡优化梯田建设成效与经验合水县水土保持工作站（７４５４６０）肖咀乡位于甘肃省合水县西南部，地处黄土高原沟壑区，全乡２０１８户，１１８３８人，３８９２名劳动力，总耕地面积２３５６ｈｍ２，其中坡耕地９７７ｈｍ２，水平梯田１４４６ｈｍ２，人均耕地０．２...