Appropriate experimental ecosystem warming methods by ecosystem, objective, and practicality

The temperature of the Earth is rising, and is highly likely to continue to do so for the foreseeable future. The study of the effects of sustained heating on the ecosystems of the world is necessary so that we might predict and respond to coming changes on both large and small spatial scales. To this end, ecosystem warming studies have been performed for more than 20 years using a variety of methods. These warming methods fall into two general categories: active and passive. Active warming methods include heat-resistance cables, infrared (IR) lamps and active field chambers. Passive warming methods include nighttime warming and passive field chambers. An extensive literature review was performed and all ecosystem warming study sites were compiled into a master list. These studies were divided by latitude and precipitation, as well as the method type used and response variables investigated. The goals of this study were to identify: (1) the most generally applicable, inexpensive and effective heating methods; and (2) areas of the world that are understudied or have been studied using only limited warming methods. It was found that the most generally applicable method, and the one that is most true to climate change predictions, is IR heating lamp installation. The least expensive method is passive chambers. The extreme lower and upper latitudes have been investigated least with ecosystem warming methods, and for the upper-mid-latitudes (60–80°) there have been limited studies published using methods other than passive chambers. Ecosystem warming method limitations and recommendations are discussed.     

Use of soil chronosequences for testing the existence of high-water-level lakes in the McMurdo Dry Valleys, Antarctica

In this study we utilize field observations and data collected from 190 pedons from Wright and Taylor Valleys to search for evidence of high-water-level lakes proposed to have existed during the Last Glacial Maximum and early Holocene (2.7–25.7 ka) in the McMurdo Dry Valleys. We hypothesize that soils above the uppermost paleo-lake level should be more strongly developed and contain more salts than soils below. During detailed mapping of soils in the Dry Valleys, we found no evidence of former lake sediments nor did we find high-level strandlines except for strandlines on the north valley wall ca. 50 m above Lake Vanda, ice-shove features, or paleo-shore features. However, there may have been minor expansions of major lakes in the McMurdo Dry Valleys. In central Taylor and Wright Valleys, soils on equivalent-aged drifts above and below the conjectured upper limits of Glacial Lakes Washburn (336 m) and Wright (550 m), respectively, are all well developed with no appreciable differences in their properties. Moreover, there were no significant differences in the slopes of regression equations relating soil property to age of the parent materials above and below the high-water lake levels.     

Changes in soil nematode populations indicate an annual life cycle at Cape Hallett, Antarctica

Soil biological studies have suggested that generations of terrestrial nematodes in continental Antarctica may take many years. We sampled soil nematodes at three sites in the Adélie penguin colony at Cape Hallett on four dates in a two month sampling period (16 November 2002–18 January 2003). The size class distribution of over 3500 nematodes, and the occurrence of adults, indicate an annual life cycle of the bacterial-feeding Panagrolaimus davidi and Plectus murrayi, at each site. Nematode abundance ranged from 2 to 1375/g dry soil. Moderate temperatures and the regular presence of free water underlie this biological activity and related contribution to soil processes.     

Distribution and abundance of soil fungi in Antarctica at sites on the Peninsula, Ross Sea Region and McMurdo Dry Valleys

Fungal abundance and diversity were studied from 245 soil samples collected in 18 distinct ice-free locations in Antarctica including areas in the McMurdo Dry Valleys, Ross Sea Region, and the Antarctic Peninsula. Cultivable fungal abundance in soil was found to be most positively correlated with percent carbon and nitrogen based on a Spearman’s rank correlation test of six soil parameters. Soil moisture and C/N ratio were also positively correlated with fungal abundance while pH and conductivity were negatively correlated. These results suggest that nutrient limitations in these highly oligotrophic environments are a primary factor in determining the distribution and abundance of indigenous fungi. Other effects of the extreme Antarctic environment likely affect fungi indirectly by limiting the distribution and abundance of plant-derived sources of carbon.     

Effects of a one-year rainfall manipulation on soil nematode abundances and community composition

Soil nematodes play a crucial role in the terrestrial nitrogen cycle by accelerating the release of ammonium from microorganisms (bacteria and fungi). As aquatic organisms, nematodes are likely to be affected by predicted changes in precipitation patterns and soil moisture during the 21st century. The objective of this study was to measure the response of soil nematodes to a one-year rainfall manipulation in the sandy, forest soils of the New Jersey Pinelands (USA). We excluded all rain from four replicate field plots and applied double the amount of natural rainfall to four additional plots. We then assessed the impact of these precipitation treatments on nematode abundance and community composition. We found that total nematode abundance increased with more precipitation, and were highly sensitive to annual precipitation amount. This is in contrast to microbial biomass which was previously found to be insensitive to precipitation change. We suggest that any increased microbial growth in high rainfall plots was consumed by microbivorous nematodes. We further suggest that nematodes in the freely draining, sandy soils we studied may be unsuccessful at surviving drought because few water-filled pore spaces remain, as compared to more aggregated soils. All nematode families were sensitive to drought, but the effect was greatest on the Plectidae, while no significant effects were found for the Cephalobidae and Qudsianematidae. While not directly measured, these results provide insight into the relative anhydrobiotic abilities of these families. We found that bacterial-feeding nematodes were most sensitive to drought, suggesting that grazer-induced alterations to the nitrogen cycle are possible if precipitation patterns change in the future.     

Responses of soil microarthropods to warming and increased precipitation in a semiarid temperate steppe

Soil microarthropods are an important component in soil food webs and their responses to climate change could have profound impacts on ecosystem functions. As part of a long-term manipulative experiment, with increased temperature and precipitation in a semiarid temperate steppe in the Mongolian Plateau which started in 2005, this study was conducted to examine effects of climate change on the abundance of soil microarthropods. Experimental warming had slightly negative but insignificant effects on the abundance of mites (−14.6%) and Collembola (−11.7%). Increased precipitation greatly enhanced the abundance of mites and Collembola by 117 and 45.3%, respectively. The response direction and magnitude of mites to warming and increased precipitation varied with suborder, leading to shifts in community structure. The positive relationships of mite abundance with plant cover, plant species richness, and soil microbial biomass nitrogen suggest that the responses of soil microarthropods to climate change are largely regulated by food resource availability. The findings of positive dependence of soil respiration upon mite abundance indicate that the potential contribution of soil fauna to soil CO₂ efflux should be considered when assessing carbon cycling of semiarid grassland ecosystems under climate change scenarios.     

Photosynthetic pigments in soils from northern Victoria Land (continental Antarctica) as proxies for soil algal community structure and function

Although soil algae are among the main primary producers in most terrestrial ecosystems of continental Antarctica, there are very few quantitative studies on their relative proportion in the main algal groups and on how their distribution is affected by biotic and abiotic factors. Such knowledge is essential for understanding the functioning of Antarctic terrestrial ecosystems. We therefore analyzed biological soil crusts from northern Victoria Land to determine their pH, electrical conductivity (EC), water content (W), total and organic C (TC and TOC) and total N (TN) contents, and the presence and abundance of photosynthetic pigments. In particular, the latter were tested as proxies for biomass and coarse-resolution community structure. Soil samples were collected from five sites with known soil algal communities and the distribution of pigments was shown to reflect differences in the relative proportions of Chlorophyta, Cyanophyta and Bacillariophyta in these sites. Multivariate and univariate models strongly indicated that almost all soil variables (EC, W, TOC and TN) were important environmental correlates of pigment distribution. However, a significant amount of variation is independent of these soil variables and may be ascribed to local variability such as changes in microclimate at varying spatial and temporal scales. There are at least five possible sources of local variation: pigment preservation, temporal variations in water availability, temporal and spatial interactions among environmental and biological components, the local-scale patchiness of organism distribution, and biotic interactions.     

Responses of soil microarthropods to changes in soil water availability in tallgrass prairie

 Changes in precipitation and soil water availability predicted to accompany global climate change would impact grasslands, where many ecosystem processes are influenced by water availability. Soil biota, including microarthropods, also are affected by soil water content, although little is known about how climate change might affect their abundance and distribution. The goal of this study was to examine soil microarthropod responses to altered soil water availability in tallgrass prairie ecosystems. Two separate experiments were done. The first utilized control and irrigated plots along a topographic gradient to examine the effects of soil water content on microarthropod densities. Microarthropods, mainly Acari, were significantly less abundant in irrigated plots and were generally less abundant at the wetter lowland sites. The second study utilized reciprocal core transplants across an east-west regional precipitation gradient. Large, intact cores were transplanted between a more mesic tallgrass site (Konza Prairie) and a more arid mixed-grass site (Hays) to determine the effects of different soil water regimes on microarthropod abundance and vertical distribution. Data from non-transplanted cores indicated greater total microarthropod densities at the drier Hays site, relative to the wetter Konza Prairie site. Data from the transplanted cores indicated significant effects of location on Acari densities in cores originating from Hays, with higher densities in cores remaining at Hays, relative to those transplanted to Konza. Acari densities in cores originating from Konza were not affected by location; however, oribatid mite densities generally were greater in cores remaining at Konza Prairie. These results confirm the importance of soil water content in affecting microarthropod densities and distributions in grasslands, and suggest complex, non-linear responses to changes in water availability. Received: 14 April 1998     

Biocrusts modulate warming and rainfall exclusion effects on soil respiration in a semi-arid grassland

Soil surface communities composed of cyanobacteria, algae, mosses, liverworts, fungi, bacteria and lichens (biocrusts) largely affect soil respiration in dryland ecosystems. Climate change is expected to have large effects on biocrusts and associated ecosystem processes. However, few studies so far have experimentally assessed how expected changes in temperature and rainfall will affect soil respiration in biocrust-dominated ecosystems. We evaluated the impacts of biocrust development, increased air temperature and decreased precipitation on soil respiration dynamics during dry (2009) and wet (2010) years, and investigated the relative importance of soil temperature and moisture as environmental drivers of soil respiration, in a semiarid grassland from central Spain. Soil respiration rates were significantly lower in the dry than in the wet year, regardless of biocrust cover. Warming increased soil respiration rates, but this response was only significant in biocrust-dominated areas (>50% biocrust cover). Warming also increased the temperature sensitivity (Q₁₀ values) of soil respiration in biocrust-dominated areas, particularly during the wet year. The combination of warming and rainfall exclusion had similar effects in low biocrust cover areas. Our results highlight the importance of biocrusts as a modulator of soil respiration responses to both warming and rainfall exclusion, and indicate that they must be explicitly considered when evaluating soil respiration responses to climate change in drylands.     

Comment on the comment by Amthor et al. on “Appropriate experimental ecosystem warming methods” by Aronson and McNulty

In a recent comment on the paper by Aronson and McNulty (2009) about “Appropriate experimental ecosystem warming methods by ecosystem, objective, and practicality”, Amthor et al. (2010) state that infrared lamps do not warm open-field plots by the mechanism expected with global warming. While this statement is correct, in the aftermath of their comment, confusion exists about how warming with infrared heaters can be related to global warming. This comment illustrates how infrared heating at “constant temperature rise” relates in a quantitative way to anticipated global warming. Amthor et al. (2010) also state that changes in vapor pressure gradient from leaf to atmosphere are an issue with infrared heating, but this problem can be minimized with supplemental irrigations in controlled amounts. Therefore, “constant temperature rise” infrared warming experiments with supplemental irrigations are a viable T-FACE (temperature free-air controlled enhancement) that can be used in combination with CO₂-FACE to produce conditions more representative of future open-fields than can be done with chambers with their many known artifacts.     

Soil biology,soil ecology,and global change

Summary This overview paper addresses aspects of scaling in space and time, and scaling in relation to micro-and macrohabitats. Ecological processes in soils are examined for possible generalizations about processes and organisms, across a wide range of different habitats. Problems of scaling in space and time that have an important impact on processes associated with global change are outlined.     

Impact of climate change on soil erosion, runoff, and wheat productivity in central Oklahoma

The potential for global climate changes to increase the risk of soil erosion is clear, but the actual damage is not. The objectives of this study were to evaluate the potential impacts of climate change on soil erosion, surface runoff, and wheat productivity in central Oklahoma. Monthly projections were used from the Hadley Centre's general circulation model, HadCM3, using scenarios A2a, B2a, and GGa1 for the periods of 1950–1999 and 2070–2099. Projected changes in monthly precipitation and temperature distributions between the two periods were incorporated into daily weather series by means of a stochastic weather generator (CLIGEN) with its input parameters adjusted to each scenario. The Water Erosion Prediction Project (WEPP) model was run for four climate scenarios including a recent historical climate and three tillage systems (conventional tillage, conservation tillage, and no-till). HadCM3-projected mean annual precipitation during 2070–2099 at El Reno, Oklahoma decreased by 13.6%, 7.2%, and 6.2% for A2a, B2a, and GGa1, respectively; and mean annual temperature increased by 5.7, 4.0, and 4.7 °C, respectively. Predicted average annual soil loss in the tillage systems other than no-till, compared with historical climate (1950–1999), increased by 18–30% for A2a, remained similar for B2a, and increased by 67–82% for GGa1. Predicted soil loss in no-till did not increase in the three scenarios. Predicted mean annual runoff in all three tillage systems increased by 16–25% for A2a, remained similar for B2a, and increased by 6–19% for GGa1. The greater increases in soil loss and runoff in GGa1 were attributed to greater variability in monthly precipitation as projected by HadCM3. The increased variability led to increased frequency of large storms. Small changes in wheat yield, which ranged from a 5% decrease in B2a to a 5% increase in GGa1, were because the adverse effects of the temperature increase on winter wheat growth were largely offset by CO₂ rise as well as the bulky decrease in precipitation occurred outside the growing season. The overall results indicate that no-till and conservation tillage systems will be effective in combating soil erosion under projected climates in central Oklahoma.     

Interactive effects of warming, soil humidity and plant diversity on litter decomposition and microbial activity

Human activity has induced a multitude of global changes that are likely to affect the functioning of ecosystems. Although these changes act in concert, studies on interactive effects are scarce. Here, we conducted a laboratory microcosm experiment to explore the impacts of temperature (9, 12 and 15 °C), changes in soil humidity (moist, dry) and plant diversity (1, 4, 16 species) on soil microbial activity and litter decomposition.We found that changes in litter decomposition did not mirror impacts on microbial measures indicating that the duration of the experiment (22 weeks) may not have been sufficient to determine the full magnitude of global change effects. However and notably, changes in temperature, humidity and plant litter diversity/composition affected in a non-additive way the microbial parameters investigated. For instance, microbial metabolic efficiency increased with plant diversity in the high moisture treatment but remained unaffected in low moisture treatment suggesting that climate changes may mask beneficial effects of biodiversity on ecosystem functioning. Moreover, litter decomposition was unaffected by plant litter diversity/composition but increased with increasing temperature in the high moisture treatment, and decreased with increasing temperature in the low moisture treatment.We conclude that it is inevitable to perform complex experiments considering multiple global change agents in order to realistically predict future changes in ecosystem functioning. Non-additive interactions highlight the context-dependency of impacts of single global change agents.     

Interactions between physical and biotic factors influence CO2 flux in Antarctic dry valley soils

Soil carbon dioxide (CO₂) flux is an integrative measure of ecosystem functioning representing both biotic and physical controls over carbon (C) balance. In the McMurdo Dry Valleys of Antarctica, soil CO₂ fluxes (approximately −0.1-0.15 μmol m⁻² s⁻¹) are generally low, and negative fluxes (uptake of CO₂) are sometimes observed. A combination of biological respiration and physical mechanisms, driven by temperature and mediated by soil moisture and mineralogy, determine CO₂ flux and, therefore, soil organic C balance. The physical factors important to CO₂ flux are being altered with climate variability in many ecosystems including arid forms such as the Antarctic terrestrial ecosystems, making it critical to understand how climate factors interact with biotic drivers to control soil CO₂ fluxes and C balances. We measured soil CO₂ flux in experimental field manipulations, microcosm incubations and across natural environmental gradients of soil moisture to estimate biotic soil respiration and abiotic sources of CO₂ flux in soils over a range of physical and biotic conditions. We determined that temperature fluctuations were the most important factor influencing diel variation in CO₂ flux. Variation within these diel CO₂ cycles was explained by differences in soil moisture. Increased temperature (as opposed to temperature fluctuations) had little or no effect on CO₂ flux if moisture was not also increased. We conclude that CO₂ flux in dry valley soils is driven primarily by physical factors such as soil temperature and moisture, indicating that future climate change may alter the dry valley soil C cycle. Negative CO₂ fluxes in arid soils have recently been identified as potential net C sinks. We demonstrate the potential for arid polar soils to take up CO₂, driven largely by abiotic factors associated with climate change. The low levels of CO₂ absorption into soils we observed may not constitute a significant sink of atmospheric CO₂, but will influence the interpretation of CO₂ flux for the dry valley soil C cycle and possibly other arid environments where biotic controls over C cycling are secondary to physical drivers.     

Soil fertility is associated with fungal and bacterial richness,whereas pH is associated with community composition in polar soil microbial communities

Microbial activities in Arctic and Antarctic soils are of particular interest due to uncertainty surrounding the fate of the enormous polar soil organic matter (SOM) pools and the potential to lose unique and vulnerable micro-organisms from these ecosystems. We quantified richness, evenness and taxonomic composition of both fungi and bacteria in 223 Arctic and Antarctic soil samples across 8 locations to test the global applicability of hypotheses concerning edaphic drivers of soil microbial communities that have been primarily developed from studies of bacteria in temperate and tropical systems. We externally validated our model's conclusions with an independent dataset comprising 33 Arctic heath samples. We also explored if our system was responding to large scale climatic or biogeographical processes that we had not measured by evaluating model stability for one location, Mitchell Pennisula, that had been extensively sampled. Soil Fertility (defined as organic matter, nitrogen and chloride content) was the most important edaphic property associated with measures of α-diversity such as microbial richness and evenness (especially for fungi), whereas pH was primarily associated with measures of β-diversity such as phylogenetic structure and diversity (especially for bacteria). Surprisingly, phosphorus emerged as consistently the second most important driver of all facets of microbial community structure for both fungi and bacteria. Despite the clear importance of edaphic factors in controlling microbial communities, our analyses also indicated that fungal/bacterial interactions play a major, but causally unclear, role in structuring the soil microbial communities of which they are a part.     

Distribution and dynamics of soil organic matter in an Antarctic dry valley

Terrestrial ecosystems in the Antarctic dry valleys function under extremely cold and dry climatic conditions that severely constrain C and N cycling and, like other polar regions, are likely to be sensitive to environmental change. To characterize the distribution and dynamics of soil organic C (SOC) and N in the various landscape elements of an Antarctic dry valley, we measured soil profile organic C and organic N stocks, inorganic N (NH₄-N and NO₃-N), soil CO₂ effluxes, water contents and soil temperatures in the Garwood Valley, a relatively small valley in southern Victoria Land. We also conducted laboratory measurements of basal respiration on soils collected from the Valley. SOC and respiration rates were low and SOC was highly stratified in the soil profile, with the largest values observed near the surface. Significant variations of SOC stocks and soil CO₂ effluxes were observed between landscape elements and spatial variability was closely related to the distance from the lake, the major site of primary production. The fastest rate of SOC turnover (residence time c. 30 years) was found in the soils at the lake edge, slower rates were found in landscape elements close to the lake (c. 52-67 years), and the slowest rates in other landscape elements (c. 84-123 years) further away. A mass balance of organic C indicates that the quantity of C fixed in the lake, accumulated on the lake edge, exposed and subsequently displaced on a 14-year basis can explain the near-surface SOC turnover within the entire valley. We conclude that the displacement of organic matter derived from the lake is an important external source for the microbial processes in these soils at a landscape scale. However, further investigations are needed in order to evaluate the importance of displaced C compared to other nutrients (e.g. N) on the spatial control of observed soil respiration rates.     

Mineralization and carbon turnover in subarctic heath soil as affected by warming and additional litter

Arctic soil carbon (C) stocks are threatened by the rapidly advancing global warming. In addition to temperature, increasing amounts of leaf litter fall following from the expansion of deciduous shrubs and trees in northern ecosystems may alter biogeochemical cycling of C and nutrients. Our aim was to assess how factorial warming and litter addition in a long-term field experiment on a subarctic heath affect resource limitation of soil microbial communities (measured by thymidine and leucine incorporation techniques), net growing-season mineralization of nitrogen (N) and phosphorus (P), and carbon turnover (measured as changes in the pools during a growing-season-long field incubation of soil cores in situ). The mainly N limited bacterial communities had shifted slightly towards limitation by C and P in response to seven growing seasons of warming. This and the significantly increased bacterial growth rate under warming may partly explain the observed higher C loss from the warmed soil. This is furthermore consistent with the less dramatic increase in the contents of dissolved organic carbon (DOC) and dissolved organic N (DON) in the warmed soil than in the soil from ambient temperature during the field incubation. The added litter did not affect the carbon content, but it was a source of nutrients to the soil, and it also tended to increase bacterial growth rate and net mineralization of P. The inorganic N pool decreased during the field incubation of soil cores, especially in the separate warming and litter addition treatments, while gross mineralized N was immobilized in the biomass of microbes and plants transplanted into the incubates soil cores, but without any significant effect of the treatments. The effects of warming plus litter addition on bacterial growth rates and of warming on C and N transformations during field incubation suggest that microbial activity is an important control on the carbon balance of arctic soils under climate change.     

Modeling soil respiration based on carbon, nitrogen, and root mass across diverse Great Lake forests

The variability in the net ecosystem exchange of carbon (NEE) is a major source of uncertainty in quantifying global carbon budget and atmospheric CO₂. Soil respiration, which is a large component of NEE, could be strongly influential to NEE variability. Vegetation type, landscape position, and site history can influence soil properties and therefore drive the microbial and root production of soil CO₂. This study measured soil respiration and soil chemical, biological and physical properties on various types of temperate forest stands in Northern Wisconsin (USA), which included ash elm, aspen, northern hardwood, red pine forest types, clear-cuts, and wetland edges. Soil respiration at each of the 19 locations was measured six times during 1 year from early June to mid-November. These data were combined with two additional data sets from the same landscape that represent two smaller spatial scales. Large spatial variation of soil respiration occurred within and among each forest type, which appeared to be from differences in soil moisture, root mass and the ratio of soil carbon to soil nitrogen (C:N). A soil climate driven model was developed that contained quadratic functions for root mass and the ratio of soil carbon to soil nitrogen. The data from the large range of forest types and site conditions indicated that the range of root mass and C:N on the landscape was also large, and that trends between C:N, root mass, and soil respiration were not linear as previously reported, but rather curvilinear. It should be noted this function appeared to level off and decline at C:N larger than 25, approximately the value where microbial nitrogen immobilization limits free soil nitrogen. Weak but significant relationships between soil water and soil C:N, and between soil C:N and root mass were observed indicating an interrelatedness of (1) topographically induced hydrologic patterns and soil chemistry, and (2) soil chemistry and root production. Future models of soil respiration should address multiple spatial and temporal factors as well as their co-dependence.     

Dominant bacteria in soils of Marble Point and Wright Valley, Victoria Land, Antarctica

A combination of culture-independent and culturing methods was used to assess the diversity of soil bacterial communities from four locations along 77 °S in Victoria Land, Antarctica. Soil samples were from the coast at Marble Point, in the Wright Valley from Bull Pass and near Lake Vanda, and from Mt. Fleming near the polar plateau. Total carbon and nitrogen, and water content of the soils were low, whereas total P was very high. The pH of the soils varied from extremely alkaline to slightly acid and electrical conductivity was medium to high on the coast and very high in inland soils from Bull Pass and Mt. Fleming. The average monthly air temperature was similar (−18 °C to −24 °C) at all the sites; however, in summer surface soil temperatures were >0 °C at Marble Point and in the Wright Valley for a total of 1100 and 1700 h, respectively. Marble Point soil had the most potential to support bacterial growth and activity with a mean total of 310 h per year when surface soils had a liquid volumetric soil moisture content >5%. Highest counts of culturable heterotrophs occurred in soil from Marble Point, whereas Mt. Fleming soil contained few organisms and had no liquid soil moisture recorded. Seven hundred and twenty-eight clones and 71 bacterial isolates were screened by restriction fragment length polymorphisms, and representatives of those dominant ribotypes that occurred more than 3 times were sequenced. The dominant ribotypes grouped within the bacterial divisions Bacteroidetes, Actinobacteria, Proteobacteria, Thermus-Deinococcus, Acidobacteria, Firmicutes and Cyanobacteria. The closest relatives of the amplicon library clones or cultured bacteria include the genera Hymenobacter, Gillisia, Arthrobacter, Rubrobacter, Friedmanniella, Deinococcus and Leptolyngbya. Many of the clones and bacteria were most similar to others from Antarctic sources, in particular a cyanobacterium-dominated cryptoendolithic community in Beacon sandstone. Some ribotypes were more prevalent in drier soils of the Wright Valley, including relatives of Deinococcus, Rubrobacter and clone FBP460 from Beacon sandstone. Bacterial communities from Marble Point soils were more diverse than those of the Wright Valley. Very few bacteria were isolated from Mt. Fleming soil.