Quality characteristics of Burley tobacco irrigated with saline water

The effect of irrigation with saline water on quality of Burley tobacco (cv. C 104) was investigated in Southern Italy over four consecutive years. A rainfed control (RC) was compared with treatments irrigated with volumes equal to crop evapotranspiration of saline waters at 0.5 (NW), 2.5 (SW₁), 5 (SW₂) and 10 (SW₃) dS m⁻¹ electrical conductivity (EC_w). In 2000 and 2001 an additional salinity treatment (15 dS m⁻¹ EC_w) was included (SW₄). The amounts of Cl⁻ added to the soil by irrigation ranged from 36.3 kg ha⁻¹ (good quality water in 1999) to 16.2 Mg ha⁻¹ (saline water at 15 dS m⁻¹ EC_w in 2000). Saline irrigation did not affect yield and yield components of cured leaves. In 1998 and 1999 the filling power of Burley tobacco did not change significantly with increasing salinity of the irrigation water. In 2000 and 2001 the filling power of SW₂, SW₃ and SW₄ treatments was significantly less than that of NW. The Cl⁻ content of tobacco grown with SW₂ was significantly greater than that grown with NW and there were no differences between SW₁ through SW₄ treatments. The filling power and the leaf Cl⁻ content were inversely related to the amount of Cl⁻ applied in the range between 40.3 kg ha⁻¹ and 5.1 Mg ha⁻¹. The filling power decreased and Cl⁻ increased up to the SW₂ treatment; beyond that level neither Cl⁻ nor filling power changed in response to increasing amounts of Cl⁻ applied. The leaf alkaloid content was unaffected by salinity. Total N was unaffected by either the growing season or the saline treatments. Cigarettes obtained from saline treatments did not burn during the smoking test in 1998. In 1999 cigarettes made from SW₁ and SW₂ did burn, but those from SW₃ did not. In 2000 and 2001 the smoking test was performed only on commercial blends containing 10 or 30% of cut tobacco from saline treatments and both blends burned similarly to cigarettes made entirely from tobacco grown under non-saline conditions. In conclusion, quality of Burley tobacco was unaffected by irrigation with saline water at 2.5 dS m⁻¹ and the inhibitory effect of salinity on burning properties could be overcome by appropriate mixture in commercial blends.     

Effect of growth regulators on yield and fiber quality in ramie (Boemheria nivea (L.) Gaud.), China grass

The effects of two mixtures of four plant growth regulators (choline chloride, gibberellin (GA₃), benzyladenine (6-BA) and NaHSO₃) at 20:9:5:800 mg kg⁻¹ (H1) and 20:42:43:2350 mg kg⁻¹ (H3) (active ingredients), respectively, were investigated on yield and fiber quality in ramie (Boehmeria nivea (L.) Gaud.). The mixtures were sprayed over the canopy at two growth stages (10 and 20 days after the previous cut) of field-grown ramie. The treatments increased raw fiber yield by 13–18%, and improved fiber fineness by 57–349 m g⁻¹, increased number of leaves per plant, and also improved all yield components. Treatment H1 resulted in a denser distribution, smaller diameters and greater quantity of fiber cells in stem cross-section. Physiological responses included improving leaf water status, increasing net photosynthetic rate, and decreasing electrolyte exosmosis rate.     

The effect of increasing NaCl in irrigation water on growth,gas exchange and yield of tobacco Burley type

Despite the economic importance of tobacco, there is limited field study on the quantitative response of growth and yield to increasing soil salinity. The effects of irrigation with saline water on yield components of field-grown tobacco (Nicotiana tabacum L.) “Burley” type plants were studied over two growing seasons. Growth, dry matter partitioning and gas exchange were measured either in rainfed or fully irrigated plants growing in a clayey–sandy–loam soil. The four fully irrigated treatments received amounts of saline waters at 0.54, 2.5, 5.0 or 10 dS m⁻¹ electrical conductivity (EC_w) equal to crop evapotranspiration. In both years, the electrical conductivity of the saturation phase (EC_e) across the 0.6 m topsoil profile increased with increasing salinity of the irrigation water. Soil moisture was markedly lower in the rainfed treatment than in fully irrigated treatments. Different saline concentrations of irrigation water had virtually no effect on soil moisture. Carbon assimilation rate, stomatal conductance and water use efficiency of the saline treatments were lower than the fully irrigated plants at 0.54 dS m⁻¹ (NW treatment) in 1996, but not in 1997. Transpiration rates were unaffected by salinity in both years. The highest yield was produced by plants irrigated with good quality water. The number of leaves per unit land area was greater for the NW plants, whereas there were no differences between the other four treatments. Salinity decreased plant dry matter and height at harvest, increased dry matter partitioning into leaves and decreased that into stems in both years. Dry matter partitioning to leaves was also greater for the rainfed plants than for the NW plants. Tobacco plants grown under field conditions showed a maximum reduction of relative yield at the highest salinity level of only 31%. The threshold values (0.56 and 0.96 dS m⁻¹) and the EC_e at which a 10% yield reduction was obtained (3.12 and 2.55 dS m⁻¹) calculated from the linear model of response of relative yield to increasing EC_e were typical of moderately sensitive crops. The EC_e values at which 50% yield was reduced (13.34 and 8.91 dS m⁻¹) were indicative of moderate tolerance to salinity.     

Modeling chickpea growth and development: Nitrogen accumulation and use

Quantitative information regarding nitrogen (N) accumulation and its distribution to leaves, stems and grains under varying environmental and growth conditions are limited for chickpea (Cicer arietinum L.). The information is required for the development of crop growth models and also for assessment of the contribution of chickpea to N balances in cropping systems. Accordingly, these processes were quantified in chickpea under different environmental and growth conditions (still without water or N deficit) using four field experiments and 1325 N measurements. N concentration ([N]) in green leaves was 50 mg g⁻¹ up to beginning of seed growth, and then it declined linearly to 30 mg g⁻¹ at the end of seed growth phase. [N] in senesced leaves was 12 mg g⁻¹. Stem [N] decreased from 30 mg g⁻¹ early in the season to 8 mg g⁻¹ in senesced stems at maturity. Pod [N] was constant (35 mg g⁻¹), but grain [N] decreased from 60 mg g⁻¹ early in seed growth to 43 mg g⁻¹ at maturity. Total N accumulation ranged between 9 and 30 g m⁻². N accumulation was closely linked to biomass accumulation until maturity. N accumulation efficiency (N accumulation relative to biomass accumulation) was 0.033 g g⁻¹ where total biomass was <218 g m⁻² and during early growth period, but it decreased to 0.0176 g g⁻¹ during the later growth period when total biomass was >218 g m⁻². During vegetative growth (up to first-pod), 58% of N was partitioned to leaves and 42% to stems. Depending on growth conditions, 37–72% of leaf N and 12–56% of stem N was remobilized to the grains. The parameter estimates and functions obtained in this study can be used in chickpea simulation models to simulate N accumulation and distribution.     

Effect of irrigation and nitrogen fertilization on biomass yield and efficiency of energy use in crop production of Miscanthus

The perennial C₄ grass Miscanthus has been proposed as a biomass energy crop in Europe. Effects of crop age, irrigation and nitrogen fertilization on biomass and energy yields and N content of Miscanthus were investigated and the energy costs of production determined. After an establishment period of 1 year, cultivation of Miscanthus resulted in a dry matter production of over 37 t ha⁻¹ year⁻¹ over a period of 4 years. Irrigation and nitrogen level greatly affected Miscanthus biomass yield. In absence of N fertilization, irrigation did not modify biomass yield and the effect of irrigation increased with the increase in N level. The average N response ranged from 37 to 50 kg biomass kg⁻¹ N applied. Because the calorific value of Miscanthus biomass (16.5 MJ kg⁻¹) was not affected by irrigation and N fertilization, energy production depended exclusively on biomass yield. Maximum energy yield was 564 GJ ha⁻¹ year⁻¹. Without N supply and irrigation, energy yield was 291 GJ h⁻¹. Net energy yield, calculated as the difference between energy output and input, but without inclusion of drying costs, was 543 GJ ha⁻¹ with N fertilization and irrigation and 284 GJ ha⁻¹ without; the ratios of energy output to input in crop production were 22 and 47, respectively.     

Effect of plant growth regulators on floral differentiation and seed production in Jojoba (Simmondsia chinensis (Link) Schneider)

The effect of foliar application of cytokinin (benzyl-adenine, 150 mg L⁻¹) and gibberellin (GA₄ + GA₇, 150 mg L⁻¹) on growth and flower development of 5-year-old plants of two jojoba clones was studied. The plant growth regulators were applied on October 5, 1999 (spring) and the plants were evaluated 120, 240 and 360 days after application. Shoot length, total number of nodes and number of nodes with branching were statistically different between clones but not between the growth regulator treatments.The total number of flowers on both clones was significantly increased by treatment with benzyl-adenine (BA) and significantly reduced by treatment with gibberellin. The seed yields, evaluated 180 days after application, were not statistically different from the control due to an increase in flower abortion. One clone treated with gibberellin showed a significant decrease in number and weight of seeds, the other did not.Histology of axillary buds revealed that BA application on one clone (4.11.32) enlarged the flower meristem, differentiating multiple flower production.     

The effect of irrigation regime on biomass and resin production in Grindelia chiloensis

Grindelia chiloensis (Corn.) Cabr. is a shrub native to Patagonia, Argentina and can accumulate as much as 25% resin in its leaves, with net primary productivity between 90 and 170 g per year per plant when growing in native stands. Under cultivation, 67.4 g of resin per plant have been produced (about 2.24 Mg ha⁻¹). The objective of this study was to assess the effect of irrigation regime on biomass and resin production on G. chiloensis. In order to achieve this objective, four irrigation treatments were performed during 1996–1997 and 1997–1998: (i) weekly irrigation (7d), (ii) irrigation at 20-day intervals (20d), (iii) irrigation at 40-day intervals (40d), (iv) non-irrigated (N-I). It was found that the intermittent water supply at 40d was sufficient to promote canopy development, and increase water use efficiency (WUE) and resin production per plant (RP) with highest resin production (approximately 5.12 Mg ha⁻¹ in 1997). In order to achieve high levels of RP, above ground biomass was maximized at the expense of a reduction in WUE. A concomitant increase in WUE (at the leaf level; WUE_L) and leaf resin content with water stress and time was found. This result supports the hypothesis that epicuticular resin could influence water transpiration (E), as it represents an additional barrier to gas diffusion from the epidermis and through the stomatal pores.     

Effects of varying nitrogen doses on yield,yield components and artemisinin content of Artemisia annua L.

Artemisia annua L. is an aromatic-antibacterial herb that destroys malarial parasites, lowers fevers and checks bleeding, and of which the secondary compound of interest is artemisinin. The objective of the present study was to determine yield, yield components and artemisinin content of A. annua L. grown under four nitrogen applications (0, 40, 80 and 120 kg ha⁻¹) in the Çukurova region of Turkey in 2004 and 2005. Field trials were conducted at Çukurova University, Agricultural Faculty Field Crops Department. In the study, plant height, number of branches, fresh herbage yield, dry herbage yield, fresh leaf yield, dry leaf yield, essential oil content and artemisinin content (by high performance liquid chromatography, HPLC) were examined. By analysis of variance, nitrogen doses had no any statistical effect on the traits investigated except for artemisinin content. Artemisinin content of the dried leaves were significantly affected by nitrogen applications, which varied from 6.32 to 27.50 mg 100 g⁻¹. Contents were from 120 and 80 kg ha⁻¹ nitrogen for the years of 2004 and 2005, respectively.     

Effects of soil water content and nitrogen supply on the productivity of Miscanthus × giganteus Greef et Deu. in a Mediterranean environment

Miscanthus × giganteus is one of the most promising biomass crops for non-food utilisation. Taking into account its area of origin (Far East), its temperature and rainfall requirements are not well satisfied in Mediterranean climate. For this purpose, a research was carried out with the aim of studying the adaptation of the species to the Mediterranean environment, and at analysing its ecophysiological and productive response to different soil water and nitrogen conditions. A split plot experimental design with three levels of irrigation (I₁, I₂ and I₃ at 25%, 50% and 100% of maximum evapotranspiration (ETm), respectively) and three levels of nitrogen fertilisation (0 kg ha⁻¹: N₀, 60 kg ha⁻¹: N₁ and 120 kg ha⁻¹: N₂ of nitrogen) were studied. The crop showed a high yield potential under well-watered conditions (up to 27 t ha⁻¹ of dry matter). M. × giganteus, in Mediterranean environment showed a high yield potential even in very limited water availability conditions (more than 14 t ha⁻¹ with a 25% ETm restoration). A responsiveness to nitrogen supply, with great yield increases when water was not limiting, was exhibited. Water use efficiency (WUE) achieved the highest values in limited soil water availability (between 4.51 and 4.83 g l⁻¹), whilst in non-limiting water conditions it decreased down to 2.56 and 3.49 g l⁻¹ (in the second and third year of experiment, respectively). Nitrogen use efficiency (NUE) decreased with the increase of water distributed (from 190.5 g g⁻¹ of I₀ to 173.2 g g⁻¹ of I₂); in relation to N fertilisation it did not change between the N fertilised treatments (N₁ and N₂), being much higher in the unfertilised control (177.1 g g⁻¹). Radiation use efficiency (NUE) progressively declined with the reduction of the N fertiliser level (1.05, 0.96 and 0.86 g d.m. MJ⁻¹, in 1994, and 0.92, 0.91 and 0.69 g d.m. MJ⁻¹, in 1995, for N₂, N₁ and N₀, respectively).     

Effect of nitrogen supply on crop conductance,water- and radiation-use efficiency of wheat

Water-use efficiency (WUE_DM) is directly related to radiation-use efficiency (RUE) and inversely related to crop conductance (g_c). We propose that reduced WUE_DM caused by shortage of nitrogen results from a reduction in RUE proportionally greater than the fall in conductance. This hypothesis was tested in irrigated wheat crops grown with contrasting nitrogen supply; treatments were 0, 80 and 120 kg N ha⁻¹ in 1998 and 0, 80, 120 and 160 kg N ha⁻¹ in 1999. We measured shoot dry matter, yield, intercepted solar radiation and soil water balance components. From these measurements, we derived actual evapotranspiration (ET), soil evaporation and transpiration, WUE_DM (slope of the regression between dry matter and ET), WUE_Y (ratio between grain yield and ET), RUE (slope of the regression between dry matter and intercepted radiation), and g_c (slope of the regression between transpiration and intercepted radiation). Yield increased from 2.3 in unfertilised to an average 4.7 t ha⁻¹ in fertilised crops, seasonal ET from 311 to 387 mm, WUE_DM from 23 to 37 kg ha⁻¹ mm⁻¹, WUE_Y from 7.6 to 12.4 kg ha⁻¹ mm⁻¹, RUE from 0.85 to 1.07 g MJ⁻¹, while the fraction of ET accounted for soil evaporation decreased from 0.20 to 0.11. In agreement with our hypothesis, RUE accounted for 60% of the variation in WUE_DM, whereas crop conductance was largely unaffected by nitrogen supply. A greater fraction of evapotranspiration lost as soil evaporation also contributed to the lower WUE_DM of unfertilised crops.     

Effect of nitrogen supply on leaf appearance,leaf growth,leaf nitrogen economy and photosynthetic capacity in maize (Zea mays L.)

Leaf area growth and nitrogen concentration per unit leaf area, N_a (g m⁻² N) are two options plants can use to adapt to nitrogen limitation. Previous work indicated that potato (Solanum tuberosum L.) adapts the size of leaves to maintain N_a and photosynthetic capacity per unit leaf area. This paper reports on the effect of N limitation on leaf area production and photosynthetic capacity in maize, a C4 cereal. Maize was grown in two experiments in pots in glasshouses with three (0.84–6.0 g N pot⁻¹) and five rates (0.5–6.0 g pot⁻¹) of N. Leaf tip and ligule appearance were monitored and final individual leaf area was determined. Changes with leaf age in leaf area, leaf N content and light-saturated photosynthetic capacity, P_max, were measured on two leaves per plant in each experiment. The final area of the largest leaf and total plant leaf area differed by 16 and 29% from the lowest to highest N supply, but leaf appearance rate and the duration of leaf expansion were unaffected. The N concentration of expanding leaves (N_a or %N in dry matter) differed by at least a factor 2 from the lowest to highest N supply. A hyperbolic function described the relation between P_max and N_a. The results confirm the ‘maize strategy’: leaf N content, photosynthetic capacity, and ultimately radiation use efficiency is more sensitive to nitrogen limitation than are leaf area expansion and light interception. The generality of the findings is discussed and it is suggested that at canopy level species showing the ‘potato strategy’ can be recognized from little effect of nitrogen supply on radiation use efficiency, while the reverse is true for species showing the ‘maize strategy’ for adaptation to N limitation.     

Effects of irrigation system and green manure on yield and nicotine content of Virginia (flue-cured) Organic tobacco (Nicotiana tabaccum), under Mediterranean conditions

Organic tobacco consists of a new industrial crop product. Field experiments were conducted to determine the effects of irrigation system and fertilization on the growth (biomass and roots) of organic tobacco plants (Nicotiana tabaccum cv NC 71). The experiment was designed as a split plot design with four replicates, two main plots (drip and sprinkler irrigation) and three sub-plots (vetch as green manure, red clover as green manure and control; without fertilization). Drip irrigation was characterised by lower amount of water applied to the soil. Furthermore the tobacco yield was not affected by the reduced water application and the crops under drip irrigation were higher than those with sprinkler irrigation. Positive effect of green manure in the nicotine content of tobacco leaves was also observed, and that reported for first time under organic system. Equally the higher amount of nitrogen from green fertilization with vetch led to increase of nicotine concentration (0.91% max concentration). Agronomic water use efficiency (WUE) for drip irrigation was always higher than those for sprinkler irrigation. There were no significant differences between the drip and sprinkler irrigation concerning the root biomass. Moreover, green manures increased roots dry weight. Yield of tobacco crop was significantly increased by the green manures, with the lowest yield (1850 kg ha^?1) to be found in the control plots. Finally, green manures increased the SPAD values and number of leaves, with most significant impact the time when vetch applied to soil.     

Radiation interception and the accumulation of biomass and nitrogen by soybean and three tropical annual forage legumes

Field experiments were conducted at Gatton and Dalby in southeastern Queensland to determine parameters associated with radiation interception and biomass and nitrogen (N) accumulation for the ley legume species, phasey bean (Macroptilum lathyroides (L.) Urban) and vigna, (Vigna trilobata (L.) Verdc.). Sesbania (Sesbania cannabina Retz.), a native legume species, and soybean (Glycine max (L.) Merrill)) were included in the study for comparison. The most important differences between species related to differences in radiation interception, radiation-use efficiency (RUE), N-accumulation efficiency and the partitioning of N to plant parts. During early growth, soybean intercepted more radiation than the other species, primarily because of its greater leaf area index (LAI). Sesbania had the highest RUE (1.08 g MJ⁻¹) followed by phasey bean (0.94 g MJ⁻¹), soybean (0.89 g MJ⁻¹) and vigna (0.77 g MJ⁻¹). The efficiency of N-accumulation was greater in soybean (0.028 g N g⁻¹) and phasey bean (0.030 g N g⁻¹) than in vigna (0.022 g N g⁻¹) and sesbania (0.021 g N g⁻¹). In all species, the proportion of N allocated to leaves declined throughout the experimental period, being more rapid in soybean than in sesbania and phasey bean. Despite this decline in total N partitioned to the leaves, both soybean and phasey bean maintained a relatively stable specific leaf nitrogen (SPLN) throughout the experimental periods although sesbania and vigna displayed rapid decreases in SPLN. The large variation between species in RUE and N-accumulation efficiency indicates that the development of ley legume cultivars with a combination of traits for more efficient legume production, water use and soil N-accumulation in the water-limited environments of the grain belt of eastern Australia may be possible. The sensitivity of forage production, water use and soil N-accumulation to variation in RUE and N-accumulation efficiency needs to be quantified using modeling techniques prior to embarking on screening programs to select appropriate germplasm for evaluation studies.     

The response of autumn and spring sown sugar beet (Beta vulgaris L.) to irrigation in Southern Italy: Water and radiation use efficiency

The Apulia region in Southern Italy is an important area for sugar beet cultivation. It is characterised by clay soils and a hot-arid and winter-temperate climate. The capability of sugar beet to exploit solar radiation, water use and irrigation supply in root yield, total dry matter and sucrose production was studied and analysed in relation to two experimental factors: sowing date – autumn (October–December) and spring (March) – and irrigation regime – optimal and reduced (respectively with 100 and 60% of actual evapotranspiration). Data sets from three experiments of spring sowing and three of autumn sowing were used to calculate: (1) water use efficiency in the conversion in dry matter (WUE_dm, plant dry matter at harvest versus seasonal water use ratio), in sucrose (WUE_suc, sucrose yield versus seasonal water use ratio); (2) irrigation water use efficiency in the conversion in dry matter (IRRWUE_dm), in sucrose (IRRWUE_suc) and fresh root yield (IRRWUE_fr); and (3) radiation use efficiency (RUE, plant dry matter during the crop cycle and at harvest versus intercepted solar radiation ratio).Autumnal beet was more productive than spring for fresh root, plant total dry matter, sucrose yield and concentration; also WUE_suc and IRRWUE_s were higher in the autumnal sugar beet, but no difference was observed in WUE_dm (on average, 2.83 g of dry matter kg⁻¹ of water used). An average saving of about 26% of seasonal irrigation supply (equivalent to about 100 mm) was measured in the three years with the earliest sowing time. The optimal irrigation regime produced higher root yield, plant total dry matter and sucrose yield than the reduced one; on the contrary the IRRWUE_fr and IRRWUE_dm were higher in the reduced irrigation strategy. WUE_s and IRRUWE_s correlated positively with the length of crop cycle, expressed in growth degree days and, in particular, to the length of the period from full soil cover canopy to crop harvest, the period when plant photosynthetic activity and sucrose accumulation are at maximum rates. Seasonal RUE was higher in the spring than in the autumn sowing (1.14 μg J⁻¹ versus 1.00 μg J⁻¹). The RUE values during the crop cycle reached the maximum in the period around complete canopy soil cover. The results showed the importance for better use of water and radiation resources of autumnal sowing time and of reduced irrigation regime in sugar beet cropped in a Mediterranean environment.     

Water balance and rice growth responses to direct seeding,deep tillage,and landscape placement: Findings from a valley terrace in Nepal

For maximizing water retention and attaining high yields, transplanting into puddled soil (TPR) is often considered the optimal method of rice (Orzya sativa L.) establishment. Alternative management techniques like direct seeding (DSR) and deep tillage have been proposed as mechanisms to improve soil physical properties for subsequent dry-season crops, but the risks to rice are uncertain. In this full factorial study on a valley terrace in Nepal, the influence of tillage (shallow—T₁, deep chisel—T₂, deep chisel + moldboard plough—T₃) and establishment practice (TPR, DSR) on the field water balance and rice performance were evaluated in two adjacent landscape settings (terrace edge “upland”, central terrace “lowland”). Although deep tillage had only modest influences on seepage and percolation (SP) rates in both years (Y₁, Y₂), landscape placement and establishment practice had significant implications for the water balance (e.g. Y₂ SP cm day⁻¹: TPR-lowland = 1.6, DSR-lowland = 2.3, TPR-upland = 4.1, DSR-upland = 6.1). During low rainfall periods, however, soil water potential and drought vulnerability were governed solely by landscape placement. Despite water balance differences, there was little evidence that rice rooting behavior was substantially modified by landscape or establishment method. Weed biomass was higher in DSR, but was uncorrelated with water balance and productivity trends. In Y₁, lower SP rates and more days with continuous flooding were positively associated with rice productivity. DSR yields were significantly lower than TPR in both landscape positions, with the lowland outperforming the upland (Y₁ mt ha⁻¹: TPR-lowland = 6.4, DSR-lowland = 5.2, TPR-upland = 5.7, DSR-upland = 4.7). To determine if N dynamics were contributing to productivity differences, fertilizer nitrogen was increased from 120 to 150 kg N ha⁻¹ in Y₂. Results suggest that DSR performance is comparable – and landscape less important – if nitrogen is non-limiting (Y₂ mt ha⁻¹: TPR-lowland = 6.9, DSR-lowland = 6.5, TPR-upland = 7.0, DSR-upland = 6.5); no aspect of the field water balance was associated with yield variability in Y₂. For direct seeding in N-deficient farming systems, landscape criteria may prove useful for minimizing production risks by identifying field areas with lower SP rates.     

Soil fertility management in irrigated rice systems in the Sahel and Savanna regions of West Africa: Part I. Agronomic analysis

Yield, input use, productivity and profitability of irrigated rice systems were analyzed based on surveys in Senegal (Thiagar and Guédé), Mali (Office du Niger) and Burkina Faso (Kou Valley). The objective was to determine agronomic factors contributing to farmers' fertilizer-use efficiency and productivity, given current farmer practices. (A second paper addresses profitability and risk issues). Grain yields were highly variable, within and across sites. Minimum grain yield was 0.2 t ha⁻¹ (Thiagar), maximum recorded grain yield was 8.7 t ha⁻¹ (Office du Niger). The yield gap between actual farmers' yield and simulated potential or maximum attainable farmers' yield ranged from 0.6 to 5.7 t ha⁻¹ (Kou), 1.8 to 8.2 t ha⁻¹ (Thiagar), 0.3 to 6.3 t ha⁻¹ (Office du Niger), 0.8 to 5.7 t ha⁻¹ (Guédé), indicating considerable scope for improved yield. Physiological nitrogen efficiency (δ grain yield/δ N uptake) was mostly between 40 and 80 kg grain kg⁻¹ plant N. Apparent recovery of fertilizer N was highly variable (average: 30–40% of applied N). Timing of N fertilizer application by farmers was extremely variable and often did not coincide with critical growth stages of the rice plant. Other agronomic constraints included: use of relatively old (>40 days) seedlings at transplanting (Kou, Office du Niger), P and/or K deficiency (Office du Niger), unreliable irrigation water supply (Kou, dry season), delayed start of the wet growing season resulting in yield losses of up to 20% due to cold-induced spikelet sterility (Kou, Guédé, Office du Niger), weed problems (Thiagar), and late harvesting (Thiagar). Discussions during meetings with farmers at the survey sites revealed that farmers lacked knowledge on (i) optimal timing, dosage and mode of fertilizer application, (ii) optimal sowing dates to avoid yield loss due to cold- or heat-induced sterility, and (iii) the importance of N as the main limiting factor to yield. Possibilities to achieve a sustainable increase in rice productivity and profitability in West African irrigation systems are discussed.     

Evapotranspiration,yield, and water-use efficiency responses of Lesquerella fendleri at different growth stages

Lesquerella fendleri (Gray) Wats. is a potential new oilseed crop for the arid southwestern United States. Lesquerella seed oil with similar properties as castor oil is being considered as a domestic replacement for the imported castor oil. Development of new crops with low irrigation needs is of high priority. Because the most critical stage of sensitivity to moisture deficits has not been determined in Lesquerella species, the objectives of this study were: (i) to identify the most critical stage or stages for moisture deficit and, (ii) to determine the effect of moisture deficit on yield, yield components, oil and fatty acid composition. Two-year field studies were conducted at the New Mexico State University, Leyendecker Plant Science Research Center. The experimental design was a randomized complete block. The treatments consisted of (a) T1: Continuous favorable soil moisture [irrigated at 50% soil water depletion (SWD)]. (b) T2: Moisture stress (75% SWD) from establishment to initial flowering with no stress from flowering to final harvest (50% SWD). (c) T3: No stress imposed from establishment to initial flowering (50% SWD) followed by stress to final harvest (75% SWD). (d) T4: Moisture stress (75% SWD) from establishment to final harvest. The amount of water applied ranged from 810 to 729 mm for the first year, and 810 to 625 mm for the second year. Seed weight per plant and number of pods per plant were generally higher when water availability was maintained at or above 50% SWD throughout the growing season. Neither seed number per pod nor seed size was influenced by irrigation treatments. Lesquerella was more sensitive to water availability during flowering and seed development as a greater loss in seed yield occurred when irrigation was delayed to 75% SWD during that stage of development. Seed yield and dry matter production from the 2 year field studies were closely related to the seasonal cumulative evapotranspiration. For each millimeter of evapotranspiration, seed yield increased from 1.8 kg ha⁻¹ mm in 1994–1995 to 1.3 kg ha⁻¹ mm for 1995–1996. The dry matter production increased 13.4 kg ha⁻¹ for each mm increase in seasonal evapotranspiration during 1994–1995. This relationship was a second order polynomial with an R² of 0.86 during 1995–1996. The WUE_gr and WUE_dm were highest under the most favorable water availability conditions for growth and seed development. Delaying irrigation to 75% SWD throughout the crop growth period resulted in the lowest oil content. Lesquerolic acid content was not affected by irrigation during both the growing seasons.     

The efficiency of nitrogen fertiliser for rice in Bangladeshi farmers’ fields

Bangladesh is currently self sufficient in rice (Oryza sativa L.), which accounts for approximately 80% of the total cropped area, and 70% of the cost of crop production. However, farmers are increasingly concerned about the perceived decline in productivity, expressed as the return on fertiliser inputs. Agronomic efficiency is a measure of the increase in grain yield achieved per unit of fertiliser input that can provide a way to quantify the observation of farmers. This study indicates that the yields achieved where only P and K fertiliser were applied ranged from 3–5 t ha⁻¹, indicating good soil fertility, particular in terms of soil N supply (37–112 kg N ha⁻¹). However, at recommended rates and at rates used by farmers, the yield response to application of fertiliser N was low. Data shows that grain yields were significantly correlated in both years (R² = 0.77 and R² = 0.67) with plant uptake in nitrogen. The internal nitrogen use efficiency seems to confirm that sink formation was limited by factors other than nitrogen. Low agronomic efficiency (5–19 kg grain kg⁻¹ N) was caused by poor internal efficiency (45–73 kg grain kg⁻¹ N), rather than low supply of soil N or loss of fertiliser N. Thus, often the applications of large amounts of N fertiliser (39–175 kg N ha⁻¹) by farmers to increase yields of high yielding variety Boro rice were not justified agronomically and ecologically. A rate of 39 kg N ha⁻¹ is very low, hardly an environmental threat. No one single factor could be identified to explain the low internal efficiency. Therefore, it is concluded that the data presented tend to confirm the indication that yields are limited by a factor other than nitrogen, which could be crop establishment, plant density, water or pest management, micro-nutrients deficiency, poor seed and transplanted seedling quality, varieties and low radiation.     

Ultrahigh CO2 levels enhances cuphea growth and morphogenesis

Applications of ultrahigh CO₂ treatments accelerated cuphea (Cuphea viscosissima × C. lanceolata ‘PSR23’) growth and development and aided in seedling establishment. The growth (fresh weight) and morphogenesis (number of leaves and roots and seedling length) were determined in cuphea seedlings exposed to 350, 1500, 3000, 10,000, or 30,000 μmol mol⁻¹ CO₂ for 30 days under greenhouse conditions. Greater CO₂ levels, especially the ultrahigh levels (i.e. ≥3000 μmol mol⁻¹ CO₂) resulted in significantly higher (P ≤ 0.05) fresh weights, leaf numbers, root numbers, and seedling lengths compared to seedlings grown under ambient air (350 μmol mol⁻¹ CO₂). For example, cuphea ‘PSR23’ Morris heavy seedlings showed the greatest seedling fresh weight, leaf number, root number, and seedling length when supplemented with 10,000 μmol mol⁻¹ CO₂ increasing 607%, 184%, 784%, and 175%, respectively, when compared to seedlings grown without CO₂ enrichment.     

Predicting maize kernel sink capacity early in development

Development of maize (Zea mays L.) kernels follows a predictable pattern involving rapid increase in dry weight and large changes in water content (WC). We showed previously that final kernel weight (KW) was closely correlated with maximum WC achieved during rapid grain filling. The objectives of the current work were (i) to test if percent moisture content (MC, measured on a fresh weight basis) could be used to normalize genetic and environmental variations in kernel development shown to affect final KW and (ii) to determine whether final KW could be predicted from kernel WC prior to rapid grain filling. The data examined included results from five hybrids varying more than 2-fold in final KW grown in the field, and from previously published results. When KW and WC were expressed relative to their maximum values obtained during kernel development, a single model described the relationship between dry weight accumulation and MC for the larger seeded hybrids (199–352 mg kernel⁻¹) and published results (222–359 mg kernel⁻¹). Two smaller seeded yellow-flint popcorn hybrids, however, accumulated less dry matter per unit moisture than expected. Nonetheless, all genotypes exhibited a common developmental relationship between kernel WC (expressed as a percent of the maximum value) and MC under well-watered conditions. A new model was developed to couple this developmental relationship to final KW. This model accurately predicted final KW from kernel WC values measured prior to rapid grain filling (∼80% MC; root mean square error, RMSE, of 28.9 mg kernel⁻¹) for all hybrids examined and all published results for which KW and kernel WC data were available. The model also provided a simple means to determine whether final KW was limited by photosynthate supply during kernel development.