共查询到20条相似文献,搜索用时 15 毫秒
1.
Camacho AT Guitian FJ Pallas E Gestal JJ Olmeda AS Habela MA Telford SR Spielman A 《Tropical animal health and production》2005,37(4):293-302
The control of equine piroplasmosis is becoming increasingly important to maintain the international market open to the horse industry. The purpose of this study was to demonstrate the occurrence of equine piroplasmosis (Theileria equi and Babesia caballi) in Galicia, north-west Spain, and to compare haematological and serum biochemistry parameters between non-parasitaemic horses and horses parasitaemic with T. equi and B. caballi. Sixty serum samples (control group) were taken from healthy horses pastured on two farms, and examined for evidence of equine T. equi and B. caballi infection by indirect fluorescent antibody test (IFAT). Of the 60 samples, 24 (40%) and 17 (28.3%) samples were positive for T. equi and B. caballi, respectively. Twelve (20%) samples were positive for both parasites. Haematology and serum biochemistry were compared between controls and a series of 36 horses clinically affected by T. equi (25) or B. caballi (11). Compared with the healthy group, there was a 43% and 37% decrease in the haematocrit for T. equi and B. caballi infection, respectively. Parasitaemic horses presented an intense anaemia and serum biochemistry signs of liver damage. The anaemia was more severe in T. equi-infected than in B. caballi-infected horses. Our results suggest that equine piroplasmosis is widespread in the region and is a cause for concern. 相似文献
2.
3.
Ozlem Ozmen Mehmet Kale Mehmet Haligur Sibel Yavru 《Tropical animal health and production》2009,41(6):951-958
In this study, pathological, serological and virological examinations were performed on 15 sheep from a flock of 250 sheep
and lambs that suffer from simultaneous naturally occurring BTV, PPRV and SPV outbreaks. SPV was diagnosed macroscopically
and histopathologically, BTV was diagnosed by ELISA, and PPRV was diagnosed pathologically and by ELISA. Clinically fever,
diarrhea, depression, polypnea, conjunctivitis, lacrimation, rhinitis, erosive stomatitis, edema of eyelids, photophobia,
cutaneous eruption with erythematous areas especially noticeable in wool-free parts of the body and axilla lesions evolving
into papules were observed. At necropsy, the most effected organs were lungs and gut. Subepicardial hemorrhages were also
commonly seen. While typical pox lesions were observed in some lambs, usually fibrinous pleuropneumonia was more prominent
lung lesion. SPV and PPRV lesions were seen at the histopathological examination of the lesioned tissues, BT lesions were
mild than SPV and PPRV microscopically. Serum and leukocyte samples of 15 animals were examined for PPRV and BTV by ELISA;
5 samples were positive for PPRV and 6 BTV, 4 were positive for both PPRV and BTV simultaneously. One hundred animals died,
most were lambs. Mortality rates were 100% in lambs and 80% in the herd. 相似文献
4.
5.
6.
Ueno TE Gonçalves VS Heinemann MB Dilli TL Akimoto BM de Souza SL Gennari SM Soares RM 《Tropical animal health and production》2009,41(4):547-552
Serum samples from 1028 sheep were collected from 32 herds within Federal District, in the central region of Brazil. The samples
were examined by indirect fluorescent antibody test (IFAT) using sera diluted 1:64 and 1:50 as cut-off values for the detection
of antibodies against Toxoplasma gondii and Neospora caninum, respectively. The observed prevalence for T. gondii infection was 38.22% (26.81%<CI 0.95<49.62%), and the titers ranged from 64 to 65536. The observed prevalence for N. caninum infection was 8.81% (7.08%<CI 0.95<10.53%). The titers ranged from 50 to 51200. The reactant sera to both pathogens corresponded
to 4.67% of the samples. The risk factors were not determined because of the absence of negative herds for T. gondii and the high proportion of positive herds for N. caninum (87.50%). The prevalence for T. gondii infection was significantly higher among males than in females. The present work is the first report on seroprevalence of
T. gondii and N. caninum in sheep from Federal District and shows that infection by both parasites is widespread in the ovine population from this
region. 相似文献
7.
8.
9.
Ruiz-Zarzuela I de Bias I Gironés O Ghittino C Múazquiz JL 《Veterinary research communications》2005,29(7):553-562
Coldwater ‘streptococcosis’, caused by Vagococcus salmoninarum, is an emerging disease of rainbow trout in the European Union, causing mortality rates up to 50% in broodstock during the spawning period, with water temperature of 10–12°C. A study to determine the presence and role of this bacterium was undertaken using classical bacteriological techniques confirmed with polymerase chain reaction. This is the first report of isolation of V salmoninarum in relation to outbreaks of mortality in a rainbow trout farm devoted exclusively to broodstock rearing in Spain. A total of 10 isolates of V salmoninarum were characterized by their morphological, cultural, physiological, biochemical and enzymatic traits. Some differences were observed in parameters such as growth on MacConkey agar, H2S production, acid production from starch, and some other minor variations. Isolates were sensitive to erythromycin and oxytetracycline tested in vitro, but treatments conducted in the field were ineffective. An attempt at vaccination did not provide encouraging results. 相似文献
10.
Rosa María Ramírez Yolanda Almanza Rafael González Santos García Norma Heredia 《Veterinary research communications》2009,33(4):379-386
Avian colisepticemia frequently occurs after respiratory tract damage, the primary site for infection allows bacteria to encounter
an exposed basement membrane, where laminin and fibronectin are important components. We investigated the ability of an isolate
of avian pathogenic Escherichia coli to bind fibronectin and laminin. Using Far-western dot blot analysis, we demonstrated the ability of this microorganism to
bind basement membrane proteins fibronectin and laminin. Results from an ELISA-based approach indicate that the binding to
these membrane proteins was bacterial-dose dependent. Furthermore, two specific E. coli polypeptides, of 32 kDa and 130 kDa, reacted with laminin and fibronectin, respectively. Further evaluation of these potential
bacterial adhesins may provide insights into the pathogenesis of colibacillosis. 相似文献
11.
Phylogenetic background of attaching and effacing <Emphasis Type="Italic">Escherichia coli</Emphasis> isolates from animals 总被引:1,自引:0,他引:1
Detection and distribution of eae gene in forty-four attaching and effacing Escherichia coli (AEEC) strains of animal origin were investigated. Association of distinct intimin alleles with phylogenetic background were assessed among strains in comparison with different serogroups. Phylogenetic analysis showed that 31 EHEC/eae+ STEC strains belong to groups A, B1 and E, 13 EPEC strains segregated in B1 and B2. Moreover, group A possessed the eae gamma2/theta type, group B1 the eae beta1, eae kappa, eae zeta, and eae epsilon types, group B2 the eae alpha1, eae alpha2 and eae iota types, while the group E possessed the eae gamma1 type. The presence of numerous eae-types show that EPEC and EHEC/eae+ STEC tested have a high genetic homology within each phylogenetic group. 相似文献
12.
Bilge Karatepe Mustafa Karatepe Ayşe Çakmak Zafer Karaer Gül Ergün 《Tropical animal health and production》2009,41(1):109-113
The prevalence of equine piroplasmosis caused by Theileria equi and Babesia caballi in Nigde, in central Anatolia, Turkey has remained unknown. Serum samples were obtained from a total of 125 horses and were
tested for antibodies to T. equi and B. caballi using the Indirect Fluorescence Antibody Test (IFAT). Twenty-three (18.4%) horses were seropositive for equine piroplasmosis.
Anti-T. equi was observed in 16 horses (12.8%) while anti-B. caballi was detected in 12 horses (9.6%). In addition, 5 serum samples were positive for both parasites. The prevalence rates of
antibodies to T. equi and B. caballi for female and male horses were statistically indifferent (p = 0.19 and 0.90). The difference between the seropositivity
rates to T. equi among age groups was statistically insignificant (p = 0.44) while the difference to B. caballi among age groups is statistically significant (p = 0.01). Seropositivity rates ranged from 2.9% to 25.7% for T. equi and 2.9% to 14.3% for B. caballi from the selected districts in Nigde. A statistically significant difference on seropositivity rates for the study sites
was observed for only T.equi (p = 0.03). This study indicates that T. equi is higher than B. caballi in Nigde.
This study was supported by the Scientific Research Projects Unit of Nigde University (FEB 2007/08). 相似文献
13.
Sharpsnout bream (Diplodus puntazzo) has been used in Adriatic aquaculture for less than a decade, but the decreasing trend of rearing this species will probably
result in its complete substitution by more exploited sea bream (Sparus aurata). Only two facilities still rear both fish species in neighbouring cages in monoculture. A switch of parasites was observed
between sparids during monitoring of the gill monogeneans of farmed fish. In wild fish of the Adriatic Sea, Lamellodiscus elegans (Monogenea: Monopisthocotylea) has previously been reported in annular (Diplodus annularis), and two-banded sea bream (D. vulgaris) and sharpsnout bream (D. puntazzo), and the present study confirmed its presence also in sea bream, in low prevalence and abundance. The exclusively sea bream
monogenean Sparicotyle chrysophrii (Monogenea: Polyopisthocotylea) was also isolated from sharpsnout bream, showing prevalence and abundance values even higher
than in its resident host. In the occurrence of L. elegans in sea bream, the opportunistic switch resulted in lower abundance and prevalence than in the original host, while in the
second case of switching the monogenean S. chrysophrii showed better reproductive capacity on a new host (sharpsnout bream). Both cases point to the possible enlargement of parasite
host range. 相似文献
14.
Fraga ME Curvello F Gatti MJ Cavaglieri LR Dalcero AM da Rocha Rosa CA 《Veterinary research communications》2007,31(3):343-353
Poultry feeds are prone to fungal growth and mycotoxin production during processing. The identification of biota with the
ability to produce mycotoxins is essential. The aims of this study were (1) to monitor the mycobiota counts at different stages
of poultry feed processing; (2) to determine the occurrence of Aspergillus species; (3) to evaluate the natural incidence of aflatoxins and ochratoxin A. The ability of Aspergillus spp. and its teleomorphs isolated here to produce these toxins was also investigated. Samples (144) were collected at random
from a factory in Brazil. The occurrence of Aspergillus and Eurotium species was demonstrated on DRBC and DG18 media and the production of aflatoxins and ochratoxin A and their natural incidence
were determined by TLC and HPLC methods. A. flavus and E. chevalieri were the most prevalent species isolated. Fungal contamination was not found after the pelleting process, though Aspergillus and Eurotium species were recovered from trough samples. High levels of aflatoxin and ochratoxin A producers were found at all stages
of poultry feed processing. Also, high natural contamination with aflatoxins and ochratoxin A was found in the samples. Contact
of feed with remainder poultry feed could lead to fungal contamination, so the risk of aflatoxin and/or ochratoxin A contamination
of feed must be taken into account. 相似文献
15.
Simón F Kramer LH Román A Blasini W Morchón R Marcos-Atxutegi C Grandi G Genchi C 《Veterinary research communications》2007,31(2):161-171
Heartworm disease caused by Dirofilaria immitis affects canine and feline hosts, with infections occasionally being reported in humans. Studies have shown that both dirofilarial
antigens and those derived from its bacterial endosymbiont Wolbachia, interact with the host organism during canine, feline and human infections and participate in the development of the pathology
and in the regulation of the host’s immune response. Both innate and acquired immune responses are observed and the development
of the acquired response may depend on the host and, or on its parasitological status. This review aims at illustrating current
research on the role of both D. immitis and Wolbachia, in the immunology and immunopathology of dirofilariosis. 相似文献
16.
Ansari-Lari M Haghkhah M Bahramy A Novin Baheran AM 《Tropical animal health and production》2009,41(4):553-557
A cross-sectional study was conducted from March to August 2006 in dairy herds in Fars province, southern Iran to determine
the herd-level risk factors for infection with Mycobacterium avium subspecies paratuberculosis (MAP). Statistical analysis using multivariable logistic regression showed that contamination of udders of periparturient
cows with manure (OR = 6.4, P = 0.02) and history of having suspected cases of Johne's disease in the herd (OR = 6.7, P = 0.04)
were significantly associated with the herd infection status. No relationship between breed, herd size and other management
practices with the infection status of the herd were found in this study. Implementing high sanitary measures in the farm,
particularly with respect to manure handling and cleaning could be considered as one of the important aspects in controlling
disease in the region as well as in the future educational effort. 相似文献
17.
The prevalence and antimicrobial resistance pattern of Salmonella isolates was determined from apparently healthy slaughtered cattle at Debre Zeit (Ethiopia). A total of 323 cattle were examined
for the presence of Salmonella in faeces, mesenteric lymph nodes, abdominal and diaphragmatic muscles. Salmonellae were cultured from 23 (7.1%) of the animals.
Salmonellae were isolated from 2 (3.1%) and 3 (4.5%) of 65 pooled faecal and mesenteric lymph node samples, respectively.
Nine (2.8%) abdominal muscle and 10 (3.1%) diaphragmatic muscle samples (n = 323 of each) were contaminated by Salmonella. About 60% of the serovars identified in the abdominal and diaphragmatic muscles were also detected from faeces and mesenteric
lymph node samples. The five different serovars isolated were Salmonella mishmarhaemek (48%), S. typhimurium (20%), S. enteritidis (12%), S. guildford (12%) and S. dublin (48%). The antimicrobial resistance profiles of 25 of the Salmonella isolates with 17 antimicrobials showed that 52% (13/25) of them were resistant to three or more antimicrobials. Both strains
of Salmonella (S. mishmarhaemek and S. typhimurium) showed multiple resistance to ampicillin, sulfamethoxazole and ticarcillin.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
18.
Larval antigen of Hyalomma anatolicum anatolicum, the vector of Theileria annulata, was purified by two-step affinity chromatography using anti-tick gut-specific rabbit IgG and IgG from immunized cattle. The purified antigen showed the presence of a single polypeptide of 37 kDa (GHLAgP) on SDS-PAGE. Two groups (I and II) of naive crossbred calves (Bos taurus × B. indicus) were immunized with 1 mg of GHLAgP in three divided doses. Immunized calves of group I were also infected with a sublethal dose of T. annulata along with a group of non-immunized calves (group III). Animals in groups I, II, III as well a control group (group IV) were challenged with live nymphs of H. a. anatolicum on the 10th day of immunization. There was a significant reduction in the number of emerging adults of 56.9% ± 1.67% in calves of group I (p < 0.01) and 63.09% ± 1.26% in calves of group II (p < 0.001) compared to the controls. The calves of groups I and II showed antibody responses to tick antigen up to day 70 post immunization. Infection with T. annulata was determined in the salivary glands of adult ticks that developed from the nymphs used for challenge infection. In ticks taken from group I calves, there was a 75.0% ± 0.00% infection compared with only 85.0% ± 2.88% infection in ticks taken from calves of group III. Using PCR, a lower infection (83.33% ± 3.33%) was detected in ticks that developed from calves of group I compared with calves from group III (90.00% ± 2.88%). The ground-up tick supernatants (GUTS) of the ticks taken from calves of group III yielded higher infection rate and exhibited higher infectivity titre in in vitro infection assay of bovine mononuclear cells than the GUTS of the ticks taken from calves of group I. The results suggest a partial reduction in growth rate of T. annulata in ticks feeding on calves immunized with GHLAgP. 相似文献
19.
Erythrocytic lipid peroxidation, activities of some antioxidant enzymes and osmotic fragility of red blood cells was studied
in adult (>1 year) crossbred cattle naturally infected with Theileria annulata. Twenty clinically healthy animals (group I) and 15 clinical cases (group II) of tropical theileriosis were selected. Cattle
suffering from theileriosis had significantly higher (p<0.01) erythrocytic lipid peroxidation and osmotic fragility. Activities of antioxidant enzymes, viz. glucose-6-phosphate
dehydrogenase (G6PD) and glutamate peroxidase (GPx) were also significantly increased (p<0.01) in group II. However, superoxide dismutase and catalase did not show significant changes. The results indicated that
infection with theileria led to increased oxidative stress to the animals, and even a significant rise in the activities of
antioxidant enzymes. G6PD and GPx could not lower this oxidative stress. However, the increase in the activities of antioxidant
enzymes pointed towards the body’s defence mechanism against lipid peroxidation during oxidative stress in theileriosis. 相似文献
20.
Zeferino Garcia-Vazquez Rodrigo Rosario-Cruz Felix Mejia-Estrada Ivan Rodriguez-Vivas Dora Romero-Salas Manuel Fernandez-Ruvalcaba Carlos Cruz-Vazquez 《Tropical animal health and production》2009,41(5):749-753
The aims of the present study were to examine the seroprevalence of neosporosis in beef herds from three southern states of
Mexico and determine the association with several risk factors. A cross-sectional serological survey for Neospora caninum was carried out by sampling a total of 596 animals from 31 herds in Chiapas, Veracruz, and Yucatan States and tested using
an ELISA assay (IDEXX) to detect anti-N. caninum antibodies. The overall prevalence was 11.6%, (95% CI: 0.93–0.14), however, the prevalence for Chiapas was 15% (30/200) (95%
CI: 0.11–0.21), in Yucatan 11.3% (21/186) (95% CI: 0.07–0.17) and in Veracruz 8.6% (18/210) (95% CI: 0.05–0.13). Of the 596
serum samples taken, 578 were from females and 18 were bulls., Only one bull sample was found seropositive from one herd in
Veracruz State. The overall herd-prevalence was 70.9% (22/31) (95% CI: 0.53–0.84), and by State was: Chiapas 90% (9/10) (95%
CI: 0.60–0.98); Yucatan 72.7% (8/12) (95% CI: 0.39–0.86); and Veracruz 50% (5/10) (95% CI: 0.24–0.76), respectively. The highest
age-prevalences were found in animals 1 yr old (19.4% [95% CI: 0.01–0.35]) and 4 yrs old (19.6% [95% CI: 0.12–0.29]), and
the lowest in animals 3 –yrs of age (6.2%,[95% CI: 0.02–0.20]). No association was found among all the variables tested in
this study since most of the ranches have similar management conditions. In conclusion, N. caninum infection is common among beef herds in the Mexican tropics. 相似文献