Deoxynivalenol, deoxynivalenol-3-glucoside, and enniatins: the major mycotoxins found in cereal-based products on the Czech market

Fusarium toxins, Alternaria toxins, and ergot alkaloids represent common groups of mycotoxins that can be found in cereals grown under temperate climatic conditions. Because most of them are chemically and thermally stable, these toxic fungal secondary metabolites might be transferred from grains into the final products. To get information on the commensurate contamination of various cereal-based products collected from the Czech retail market in 2010, the occurrence of "traditional" mycotoxins such as groups of A and B trichothecenes and zearalenone, less routinely determined Alternaria toxins (alternariol, alternariol monomethyl ether and altenuene), ergot alkaloids (ergosine, ergocryptine, ergocristine, and ergocornine) and "emerging" mycotoxins (enniatins A, A1, B, and B1 and beauvericin) were monitored. In a total 116 samples derived from white flour and mixed flour, breakfast cereals, snacks, and flour, only trichothecenes A and B and enniatins were found. Deoxynivalenol was detected in 75% of samples with concentrations ranging from 13 to 594 μg/kg, but its masked form, deoxynivalenol-3-β-d-glucoside, has an even higher incidence of 80% of samples, and concentrations ranging between 5 and 72 μg/kg were detected. Nivalenol was found only in three samples at levels of 30 μg/kg. For enniatins, all of the samples investigated were contaminated with at least one of four target enniatins. Enniatin A was detected in 97% of samples (concentration range of 20-2532 μg/kg) followed by enniatin B with an incidence in 91% of the samples (concentration range of 13-941 μg/kg) and enniatin B1 with an incidence of 80% in the samples tested (concentration range of 8-785 μg/kg). Enniatin A1 was found only in 44% of samples at levels ranging between 8 and 851 μg/kg.     

Biosynthesis of 15N3-labeled enniatins and beauvericin and their application to stable isotope dilution assays

The first stable isotope dilution assay for the determination of enniatins A, A1, B, and B1 and beauvericin was developed. The (15)N(3)-labeled enniatins and beauvericin were biosynthesized by feeding two Fusarium strains Na(15)NO(3) and subsequently isolated from the fungal culture. The chemical structures of the biosynthesized products were characterized by LC-MS/MS and (1)H NMR. Standard solutions of (15)N(3)-labeled beauvericin, enniatin A, and enniatin A1 were accurately quantitated by quantitative NMR. On the basis of the use of the labeled products as internal standards, stable isotope dilution assays were developed and applied to various food samples using LC-MS/MS. The sample extracts were directly injected without any tedious cleanup procedures. The limits of detection were 3.9, 2.6, 3.7, 1.9, and 4.4 μg/kg for enniatins A, A1, B, and B1 and beauvericin, respectively. Limits of quantitation were 11.5 (enniatin A), 7.6 (enniatin A1), 10.9 (enniatin B), 5.8 (enniatin B1), and 13.1 μg/kg (beauvericin). Recoveries were within the range between 90 and 120%, and good intraday and interday precisions with coefficients of variation between 1.35 and 8.61% were obtained. Thus, the stable isotope dilution assay presented here is similarly sensitive and precise but more accurate than assays reported before. Analyses of cereals and cereal products revealed frequent contaminations of barley, wheat, rye, and oats with enniatins B and B1, whereas beauvericin was not quantifiable.     

Simultaneous determination of beauvericin, enniatins, and fusaproliferin by high performance liquid chromatography

A rapid, sensitive and inexpensive HPLC method for routine screening of beauvericin, fusaproliferin, and enniatin B(1), A(1), and B has been optimized. Detection limits were determined, ranging between 0. 5 and 3.6 ng according to the compound obtained after spiking samples with each mycotoxin at 10-56 microg/mL concentration range; recoveries averaging from 56 to 74% were obtained. LC-MS conditions for enniatin analyses by API electrospray technique were set up, this allowing a unique identification of three different enniatins.     

Ultrahigh-performance liquid chromatographic-tandem mass spectrometric multimycotoxin method for quantitating 26 mycotoxins in maize silage

A multianalyte method was developed to identify and quantitate 26 mycotoxins simultaneously in maize silage by means of ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The extraction and cleanup procedure consists of two extraction steps followed by purification on a Waters Oasis HLB column. The method developed was validated with the requirements of Commission Decision 2002/657/EC taken into account. The limit of detection and quantitation ranges were 5-348 and 11-695 ng/g, respectively. Apparent recovery varied between 61 and 116%, whereas repeatability and reproducibility were within the ranges of 3-45 and 5-49%, respectively. The method developed was successfully applied for maize silage samples taken at the cutting surface and 1 m behind that surface. Mainly Fusarium toxins (beauvericin, deoxynivalenol, enniatins, fumonisins, fusaric acid, and zearalenone) were detected, but postharvest toxins such as mycophenolic acid and roquefortine C were identified as well.     

Quantitation of Mycotoxins in Food and Feed from Burkina Faso and Mozambique Using a Modern LC-MS/MS Multitoxin Method

In this study an LC-MS/MS multitoxin method covering a total of 247 fungal and bacterial metabolites was applied to the analysis of different foods and feedstuffs from Burkina Faso and Mozambique. Overall, 63 metabolites were determined in 122 samples of mainly maize and groundnuts and a few samples of sorghum, millet, rice, wheat, soy, dried fruits, other processed foods and animal feeds. Aflatoxin B(1) was observed more frequently in maize (Burkina Faso, 50% incidence, median = 23.6 μg/kg; Mozambique, 46% incidence, median = 69.9 μg/kg) than in groundnuts (Burkina Faso, 22% incidence, median = 10.5 μg/kg; Mozambique, 14% incidence, median = 3.4 μg/kg). Fumonisin B(1) concentrations in maize were higher in Mozambique (92% incidence, median = 869 μg/kg) than in Burkina Faso (81% incidence, median = 269 μg/kg). In addition, ochratoxin A, zearalenone, deoxynivalenol, nivalenol, and other less reported mycotoxins such as citrinin, alternariol, cyclopiazonic acid, sterigmatocystin, moniliformin, beauvericin, and enniatins were detected. Up to 28 toxic fungal metabolites were quantitated in a single sample, emphasizing the great variety of mycotoxin coexposure. Most mycotoxins have not been reported before in either country.     

Liquid chromatographic determination of fumonisins B1, B2, and B3 in corn silage

Corn silage was dried, ground, and then extracted with 0.1 M ethylenediaminetetraacetic acid. The filtrate was applied to a FumoniTest immunoaffinity column. Fumonisins were derivatized with naphthalene-2,3-dicarboxaldehyde, separated on a C(18) liquid chromatographic column, and detected by fluorescence. The detection limits for fumonisin B(1), fumonisin B(2), and fumonisin B(3) were 50, 25, and 25 ng/g of dried silage, respectively. Recoveries of fumonisin B(1), fumonisin B(2), and fumonisin B(3) from wet and dried corn silage spiked over the range of 100-5000 ng/g averaged 91-106%. The method was applied to corn silage samples collected from the midwestern area of the United States during 2001-2002. Of 89 corn silage samples, fumonisin B(1), fumonisin B(2), and fumonisin B(3) were found in 86 (97%), 64 (72%), and 51 (57%) of the samples. The mean positive levels of fumonisin B(1), fumonisin B(2), and fumonisin B(3) were 615, 93, and 51 ng/g, respectively, in dried silage. This suggests that fumonisins may be frequent low level contaminants in corn silage.     

瘤胃液对全株甜高粱青贮质量的影响

甜高粱是一种重要的能源作物,为实现长时间贮存并提升糖化效率,该研究分析了瘤胃液不同添加量对全株甜高粱青贮品质和酶解糖化效果的影响。设置R1、R3、R5和R7共4个瘤胃液处理组(添加量依序分别为1、3、5和7 mL/100 g原料)和1个对照组(CK,等量蒸馏水),考察了瘤胃液不同添加量对全株甜高粱青贮过程中有机组分、发酵品质和酶解性能等质量指标的动态影响,并跟踪解析青贮期间微生物菌群的动态演绎。结果表明,添加瘤胃液能明显减少青贮甜高粱中的干物质、水溶性碳水化合物、粗蛋白以及木质纤维组分含量,使青贮pH和氨氮含量显著下降(P0.05),并与瘤胃液添加量呈负相关。青贮中的乳酸、乙酸含量随瘤胃液添加量和青贮发酵时间延长而明显增加(P0.05),瘤胃液强化了青贮发酵并有助于减少干物质损失,尤其在较高添加量时,青贮60d时的甜高粱综纤维素含量反而有所增加。4种瘤胃液处理组的门水平优势细菌主要为厚壁菌和变形菌,厚壁菌相对丰度随时间延长和瘤胃液添加量的增加而逐渐增加,变形菌门丰度则逐渐下降;属水平主要以乳酸杆菌、泛菌和醋酸杆菌为主,乳酸杆菌丰度与时间、瘤胃液添加量呈正相关,而泛菌则呈减少趋势。瘤胃液强化青贮后的甜高粱还原糖得率显著提升,尤其瘤胃液添加量为7 mL/100 g的R7处理组的糖得率较原料分别提高了11.06%(30 d)和19.28%(60 d)。添加瘤胃液能有效改善青贮甜高粱的发酵质量和生物降解性能,起到生物强化的预处理作用,为甜高粱的乙醇化利用奠定了基础。     

A validated multianalyte LC-MS/MS method for quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples

This study was designed to develop a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous detection and quantification of 25 mycotoxins in cassava flour, peanut cake and maize samples with particular focus on the optimization of the sample preparation protocol and method validation. All 25 mycotoxins were extracted in a single step with a mixture of methanol/ethyl acetate/water (70:20:10, v/v/v). The method limits of quantification (LOQ) varied from 0.3 μg/kg to 106 μg/kg. Good precision and linearity were observed for most of the mycotoxins. The method was applied for the analysis of naturally contaminated peanut cake, cassava flour and maize samples from the Republic of Benin. All samples analyzed (fifteen peanut cakes, four maize flour and four cassava flour samples) tested positive for one or more mycotoxins. Aflatoxins (total aflatoxins; 10-346 μg/kg) and ochratoxin A (相似文献   

Fate of the Cry1Ab protein from Bt-maize MON810 silage in biogas production facilities

Biogas plants fuelled with renewable sources of energy are a sustainable means for power generation. In areas with high infestation levels with the European corn borer, Ostrinia nubilalis (Hbn.), it is likely that transgenic Bt-maize will be fed into agricultural biogas plants. The fate of the entomotoxic protein Cry1Ab from MON810 maize was therefore investigated in silage and biogas production-related materials in the utilization chains of two farm-scale biogas plants. The Cry1Ab content in silage exhibited no clear-cut pattern of decrease over the experimental time of 4 months. Mean content for silage was 1878 +/- 713 ng Cry1Ab g(-1). After fermentation in the biogas plants, the Cry1Ab content declined to trace amounts of around 3.5 ng g(-1) in the effluents. The limit of detection of the employed ELISA test corresponded to 0.75 ng Cry1Ab g(-1) sample material. Assays with larvae of O. nubilalis showed no bioactivity of the reactor effluents. The utilization of this residual material as fertilizer in agriculture is therefore deemed to be ecotoxicologically harmless.     

Reduction in fusarium toxin levels in corn silage with low dry matter and storage time

Under unfavorable climatic conditions, Fusarium spp. can contaminate corn plants in the field and produce toxins that are present at the time of ensiling. The stability of deoxynivalenol, fumonisins B1 and B2, and zearalenone in corn silage was tested over two consecutive years. Variables studied were corn dry matter (DM) and storage length and temperature. The concentration of all Fusarium toxins decreased upon ensiling ( P < 0.001). Increasing the length of storage and ensiling with low DM resulted in a higher rate of toxin disappearance, particularly for the water soluble toxins deoxynivalenol and fumonisin B1. Toxin disappearance ranged from 50% for zearalenone to 100% for deoxynivalenol. In contrast, temperature did not have any effect on stability ( P > 0.05). These results indicate that low DM at ensiling as well as a prolonged storage could be a practical way to reduce or eliminate some Fusarium toxins in contaminated silages.     

云南省玉米间作蔬菜和牧草对坡地土壤侵蚀的影响

2005—2006年在云南农业大学试验农场10°坡地上研究了玉米间作蔬菜和牧草对径流、土壤侵蚀的影响。结果表明,间作的水土保持效果优于玉米单作。2005年径流和土壤侵蚀,间作比玉米单作分别减少24.4%～34.1%和13.0%～50.9%;2006年间作比玉米单作分别减少8.5%～55.3%和32.5%～85.3%。其中玉米间作牧草水土保持效果最好,其次是玉米间作马铃薯及间作甘蓝。2005年径流量与降雨量、降雨强度和降雨侵蚀力呈极显著正相关;土壤侵蚀量与降雨强度和降雨侵蚀力呈极显著正相关关系。     

Natural occurrence of fumonisins B(1) and B(2) in domestic maize of Taiwan.

Samples of maize grown in various districts of Taiwan were collected and analyzed for the presence of fumonisin B(1) (FB(1)) and fumonisin B(2) (FB(2)) using high-performance liquid chromatography. Forty-nine (44.5%) and 2 (1.8%) of 110 samples were found to contain FB(1) (109-1148 ng/g) and FB(2) (222-255 ng/g), respectively. The frequency of detection and also the maximum FB(1) concentration were found in samples from Penton (2/2, 262 ng/g), followed by Chiayi (18/26, 264 ng/g), Tainan (8/16, 160 ng/g), Hualinen (5/14, 1148 ng/g), Taitung (7/20, 109 ng/g), and Yunlin (9/26, 361 ng/g). Of the 110 samples examined, only 2 samples from Hualinen had been detected containing FB(2). During an analysis of the distribution pattern of FB(1), it became apparent that >79% of tested samples had FB(1) concentrations <100 ng/g, whereas 2.7% (or 3 samples) contained FB(1) >300 ng/g. These results clearly illustrated that domestically produced maize for human consumption is frequently contaminated with FB(1).     

不同施氮水平下青贮玉米农田土壤酶活性时空分布特征

【目的】研究氮肥施用下青贮玉米田土壤酶活性时空变化特征,为内蒙古中部青贮玉米高产高效栽培提供理论依据。【方法】以“种星青饲1号”为供试材料,设置0(N0)、120 (N8)、180(N12)、240(N16)、300(N20)和360(N24) kg hm^-26个氮肥梯度,系统分析2018～2019年不同处理青贮玉米田土壤酶活性在5个生育期和0～40 cm土体的时空变化特征,并探究了青贮玉米产量对施氮水平的响应。【结果】青贮玉米田土壤酶活性在不同施氮处理、生育期于0～40 cm土体中存在显著差异(P <0.05),随着玉米生育期的延长,青贮玉米田土壤酶活性呈“抛物线”型变化,大喇叭口期-抽雄期最高。相比0～20 cm土层,较深的土层增加了过氧化氢酶(CAT)活性的同时降低了脲酶(UA)活性。增施氮肥有效促进蔗糖酶(IA)、碱性磷酸酶(APA)、脲酶活性及玉米产量的增加,且施氮量为240 kg hm^-2(N16)时集中呈现出酶活性及产量峰值。2018年与2019年N16处理玉米生物鲜重分别较其他处理高出了8.07%～65.88%和7.11...     

Mycotoxin production by Fusarium avenaceum strains isolated from Norwegian grain and the cytotoxicity of rice culture extracts to porcine kidney epithelial cells

The secondary metabolites of 24 isolates of Fusarium avenaceum from Norwegian cereals and grown on rice have been characterized. Moniliformin (MON), enniatins (ENNs), and beauvericin (BEA) were analyzed by high-performance liquid chromatography. Porcine kidney epithelial cells (PK15, American Type Culture Collection) were used to study the cytotoxicity of MON in the extracts. The following metabolites were produced by all isolates, ranked by concentration in rice cultures: ENN-B, MON, ENN-B1, and ENN-A. BEA was produced by eight isolates. The productions of BEA and ENN-A were significantly correlated, as was the case with ENN-B and ENN-B1. MON production was correlated neither to any of the other toxins nor to toxicity.     

Analysis of moniliformin in maize plants using hydrophilic interaction chromatography

A novel HPLC method was developed for detection of the Fusarium mycotoxin, moniliformin in whole maize plants. The method is based on hydrophilic interaction chromatography (HILIC) on a ZIC zwitterion column combined with diode array detection and negative electrospray mass spectrometry (ESI(-)-MS). Samples were extracted using acetonitrile-water (85:15), and the extracts were cleaned up on strong anion exchange columns. By this procedure we obtained a recovery rate of 57-74% moniliformin with a limit of detection at 48 ng/g and a limit of quantification at 96 ng/g using UV detection at 229 nm, which is comparable to current methods used. Limit of detection and quantification using ESI(-)-MS detection was 1 and 12 ng/g, respectively. Screening of maize samples infected with the moniliformin producing fungi F. avenaceum, F. tricinctum, or F. subglutinans detected moniliformin levels of 1-12 ng/g in 15 of 28 samples using ESI(-)-MS detection. To our knowledge this is the first example of HILIC separation in mycotoxin analysis.     

玉米秸秆与废弃白菜的混合青贮品质及产沼气能力分析

为实现干玉米秸秆(dried maize straw,DMS)的长时间保质贮存,利用青贮原理将DMS与废弃白菜(cabbage waste,CW)连续混贮90 d,设置DMS单贮组和6个不同质量比例(DMS:CW=29:19、27:21、25:23、23:25、21:27和19:29)混贮组,间隔30 d分析其贮存品质,筛选适宜贮存条件,并考察秸秆贮存前后的微观结构变化和产沼气潜力。结果表明,与单贮组相比,6个混贮组的p H值均显著下降(P0.05),乳酸含量显著升高(P0.05),混贮品质优于单贮。6个混贮组贮存30 d时的p H值均为最低,乳酸含量最高,感官评价均为优级,30 d后p H值有所上升,乳酸含量急剧下降,60d时的木质素含量显著降低(P0.05),综纤维素含量显著升高(P0.05)。随着CW比例增加,混贮组p H值逐渐减小,乳酸含量逐渐增加,MEⅤ组(DMS:CW=21:27)p H值最低,乳酸含量最高,能使DMS连续贮存60 d不变质。扫描电镜结果显示,青贮发酵使干秸秆原先致密复杂的木质纤维结构开始瓦解,青贮秸秆表面有很多裂缝和孔洞,与微生物或酶的可接触面积增加。沼气发酵试验表明,MEⅤ组混贮秸秆的累积产气量略高于未贮存干秸秆,且累积甲烷产量提高了61.67%,是未贮存干秸秆的1.6倍。Modified Gompertz模型显示混贮秸秆的甲烷生成速率增加,产甲烷能力提高。总之,当DMS∶CW质量比为21∶27(含水率为73%)时DMS能连续保质贮存2月,且贮存后秸秆的甲烷产量明显提升。     

基于高光谱成像技术的青贮玉米饲料pH值无损检测

为实现青贮玉米饲料pH值的快速、无损检测,该研究采用高光谱成像技术建立不同品质青贮玉米饲料pH值的定量检测模型。采集青贮玉米饲料样本936～2 539nm的平均光谱,采用6种预处理方法对青贮玉米饲料平均光谱进行处理,通过建立偏最小二乘回归（partialleastsquaresregression,PLSR）模型得出多元散射校正（multiplicativescatter correction,MSC）和卷积平滑（savitzky-golay,SG）两种预处理方法效果较好,使用竞争性自适应重加权算法（competitive adaptive reweighted sampling,CARS）、变量组合集群分析算法（variable combination population analysis,VCPA）以及迭代保留信息变量（iteratively retains informative variables,IRIV）算法对经MSC和SG卷积平滑预处理光谱进行特征波长提取,利用PLSR和极限学习机（extreme learning machines,ELM）分别建立饲料全波段、特征波长...     

微咸水灌溉对土壤盐分平衡与作物产量的影响

河北低平原淡水资源短缺,微咸水资源丰富,合理开发利用微咸水已经成为缓解水资源供需矛盾的重要途径之一。本研究于2011—2015年在河北省沧州市中国科学院南皮生态农业试验站进行,以冬小麦和夏玉米一年两熟种植体系为研究对象,开展了河北低平原区实施微咸水灌溉对冬小麦及下茬作物夏玉米产量及灌溉对土壤盐分周年平衡的影响。2013—2014年冬小麦灌溉处理设雨养旱作处理(CK)、拔节期淡水灌溉1水(F1)、拔节期用2 g·L~(-1)、3 g·L~(-1)、4 g·L~(-1)、5 g·L~(-1)的微咸水灌溉1次(B21、B31、B41、B51)、拔节期和灌浆期用淡水灌溉(F2)、拔节期用3 g·L~(-1)的微咸水+灌浆期用淡水灌溉(B31F1)、拔节期用淡水+灌浆期用3 g·L~(-1)微咸水灌溉(F1B31)、拔节期和灌浆期都用3 g·L~(-1)的微咸水灌溉(B32)、拔节期、抽穗期和灌浆期都用淡水灌溉(F3)。2014—2015年根据上年度的试验结果对试验处理进行了精简,冬小麦灌溉处理设CK、F1、B31、B41、B51、B42(拔节期和灌浆期都用4 g·L~(-1)的微咸水灌溉)。结果表明,一般年型下冬小麦生育期灌溉2水就能获得高产和稳产,平均产量为6 593.4 kg·hm~(-2)。利用小于5 g·L~(-1)的微咸水灌溉,与淡水灌溉相比,不会造成冬小麦产量降低,灌溉1次微咸水比雨养旱作处理增产10%~30%,可用微咸水替代1次淡水。微咸水灌溉条件下冬小麦收获时土壤盐分有所积累,表层土壤含盐量大于1 g·L~(-1),影响下茬玉米的出苗和生长,但夏玉米播种后用675~750 m3·hm-2淡水灌溉可满足耕层淋盐需求,达到玉米生长的安全阈值,与淡水灌溉处理的玉米产量相比不减产。利用夏季降雨,可使土壤盐分得到淋洗,当夏季降雨量大于300 mm时,冬小麦微咸水灌溉下土壤盐分达到周年平衡。沧州地区73%以上的年份,夏季降雨量大于300 mm,为土壤淋盐创造了条件,保证了微咸水替代一次淡水灌溉的安全性。     

内蒙古东部中晚熟区青贮玉米产量和品质与气象因素的关系

为研究青贮玉米(Zea mays L.)产量和品质与气象因素的关系,于2018—2019年在通辽市农业科学研究院试验基地,以北农青贮368、东科301、京科青贮516及京科968为材料,设置D1(4月24日)、D2(5月1日)、D3(5月8日)和D4(5月15日)4个播期,比较不同播期条件下青贮玉米产量和品质的变化,分析青贮玉米产量和品质形成与气象因素的关系。结果表明,随着播期的推迟,各品种青贮玉米生育期缩短,株高和茎粗均呈增加趋势,绿叶数变化不大,D2播期的穗位较高。各品种在D2播期的平均生物产量最高(35.2～40.2 t·hm^-2)。随着播期推迟,中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)含量呈增加趋势,粗蛋白含量变化不明显,D2播期淀粉含量较高。生育期日照时数(R²=0.381,P=0.031)和吐丝后日照时数(R²=0.507,P=0.003)与干物质产量呈负相关,生育期降雨量(R²=0.591,P<0.001)和吐丝后降雨量(R²=0.367,P=0.03...     

基于改进离散粒子群算法的青贮玉米原料含水率高光谱检测

快速、无损和准确检测青贮玉米原料含水率,对确保青贮玉米发酵品质、推动青贮产业健康快速发展有重要现实意义。为探究高光谱技术在青贮玉米原料含水率检测方面的可行性,研究通过高光谱成像系统获取青贮玉米原料高光谱图像并利用烘箱加热法测定实际含水率。在粒子更新方式和惯性权重2个方面对传统离散粒子群算法(discretebinary particle swarm optimization,DBPSO)进行优化,提出基于改进型离散粒子群算法(modified discrete binary particle swarm optimization,MDBPSO)的特征波段优选方法,并利用相关系数分析法(correlation coefficient,CC)、DBPSO和MDBPSO法提取原料含水率高光谱特征变量,基于全波段反射光谱(total spectral reflectance,TSR)和特征波段反射光谱建立青贮玉米原料含水率预测模型。结果表明,MDBPSO优选特征波段适应度函数的收敛精度和收敛效率较DBPSO法均有明显改善,最优适应度值由0.761 6提高至0.812 3,函数收敛迭代次数由280次降低至79次。MDBPSO-PLSR预测模型的建模精度和预测精度均高于CC-PLSR、DBPSO-PLSR和TSR-PLSR预测模型,其校正集决定系数Rc2和均方根误差RMSEC(root mean square error of calibration)分别为0.81和0.032,预测集决定系数Rp2和均方根误差RMSEP(root mean square error of prediction)分别为0.80和0.045。该研究表明,利用高光谱图像技术检测青贮玉米原料含水率具有较高的精度,研究可为后续开发青贮玉米原料水分快速检测仪器提供借鉴方法。