Moisture-dependent physical properties of jatropha seed (Jatropha curcas L.)

The study was conducted to investigate some moisture-dependent physical properties of jatropha seed namely, seed dimension, 1000 seed mass, surface area, sphericity, bulk density, true density, angle of repose and static coefficient of friction against different materials. The physical properties of jatropha seed were evaluated as a function of moisture content in the range of 4.75–19.57% d.w. The average length, width, thickness and 1000 seed mass were 18.65 mm, 11.34 mm, 8.91 mm and 741.1 g, respectively at moisture content of 4.75% d.w. The geometric mean diameter and sphericity increased from 12.32 to 12.89 mm and 0.66 to 0.67 as moisture content increased from 4.75 to 19.57% d.w., respectively. In the same moisture range, densities of the rewetted jatropha seed decreased from 492 to 419 kg m⁻³, true density increased from 679 to 767 kg m⁻³, and the corresponding porosity increased from 27.54 to 45.37%. As the moisture content increased from 4.75 to 19.57% d.w., the angle of repose and surface area were found to increase from 28.15° to 39.95° and 476.78 to 521.99 mm², respectively. The static coefficient of friction of jatropha seed increased linearly against the surfaces of three structural materials, namely plywood (44.12%), mild steel sheet (64.15%) and aluminum (68.63%) as the moisture content increased from 4.75 to 19.57% d.w.     

Moisture-dependent physical properties of Karanja (Pongamia pinnata) kernel

The moisture-dependent physical properties are important to design post harvest equipments of the product. The physical properties of Karanja kernel were evaluated as a function of moisture content in the range of 8.56–22.22% d.b. The average length, width, thickness and 1000 kernel mass were 25.29 mm, 15.58 mm, 7.88 mm and 1036.45 g, respectively, at moisture content of 8.56% d.b. The geometric mean diameter and sphericity increased from 14.55 to 15.97 mm and 0.57 to 0.6 as moisture content increased from 8.56 to 22.22% d.b., respectively. In the same moisture range, the bulk density decreased from 663 to 616 kg/m³, whereas the corresponding true density and porosity increased from 967 to 1081 kg/m³ and 31.44 to 43.02%, respectively. As the moisture content increased from 8.56 to 22.22% d.b., the angle of repose and surface areas were found to increase from 27.69 to 37.33° and 665.74 to 801.63 mm², respectively. The static coefficient of friction of Karanja kernel increased linearly against the surfaces of three structural materials, namely plywood (28.72%), mild steel sheet (29.88%) and aluminium (18.86%) as the moisture content increased from 8.56 to 22.22% d.b.     

Valorization of three varieties of grape

In the present investigation, seed methanolic extracts of three Vitis vinifera (Muscat d’Italie, Syrah and Carignan) were assayed for their antioxidant activities. Results showed that there are strong variations in the contents of total phenols (440.97–121.94 mg GAE g⁻¹ DW), flavonoids (48.07–16.81 mg EC g⁻¹ DW) and tanins (37.15–14.9 mg EC g⁻¹ DW) from the studied seeds. The phenolic composition of these extracts was determined by RP–HPLC after acid hydrolysis. The main phenolic compound was quercetin with 27.2% in Muscat d’Italie, 48.8% in Syrah and 28.4% in Carignan. Besides, all seed extracts showed remarkable DPPH radical scavenging activity with IC₅₀ values ranged from 1.8 to 30 μg ml⁻¹. EC₅₀ values of reducing power activity ranged from 100 to 120 μg ml⁻¹. The high phenolic content and the considerable antioxidant activities of vitis seed extracts could potentially be considered as an expensive source of natural antioxidants.     

Some physical properties of rough rice (Oryza Sativa L.) grain

Designing the equipment for processing, sorting, sizing and other post-harvesting equipment of agricultural products requires information about their physical properties. In this study, various physical properties of rough rice cultivars were determined at a moisture content of 10% (wet basis). In the case of Sorkheh cultivar, the average thousand grain weight, equivalent diameter, surface area, volume, sphericity, aspect ratio, true density, bulk density and porosity were 21.64 g, 3.35 mm, 31.76 mm², 20.27 mm³, 39.71%, 0.28, 1269.1 kg/m³, 544.34 kg/m³, and 56.98%, respectively. The corresponding values were 20.52 g, 3.4 mm, 32.58 mm², 21.06 mm³, 39.88%, 0.29, 1193.38 kg/m³, 471.21 kg/m³, and 60.37% for Sazandegi cultivar. For Sorkheh cultivar, the average static coefficient of friction varied from 0.2899 on glass to 0.4349 on plywood, while for Sazandegi cultivar the corresponding value varied from 0.2186 to 0.4279 on the same surfaces. Angle of repose values for Sorkheh and Sazandegi cultivars were 37.66° and 35.83°, respectively. Linear model for describing the mass of rough rice grain was investigated. Mass was estimated with single variable of kernel length with a determination coefficient as 0.862 for Sorkheh cultivar whereas for Sazandegi cultivar was as 0.860.     

Physical properties of tung seed: An industrial oil yielding crop

Data on physical properties of seeds have significant importance for machinery and process equipments design. This study was conducted to investigate some physical properties of tung seed (Aleutites Fordii) namely, dimensions, 100 unit mass, arithmetic mean diameter, geometric mean diameter, sphericity, aspect ratio, surface area, bulk density, true density, porosity, terminal velocity and coefficient of friction. The applications of these properties are also discussed. The tung seed has an average of 13.24% (d.b.) moisture and 40.37% oil content. The average seed length, width, thickness were 22.61 mm, 20.35 mm, 13.95 mm, respectively. The average surface area of tung seed is 1084.20 mm² while the sphericity and aspect ratio were 0.82 and 90.07%, respectively. The average bulk density of seed was 0.502 g/cm³ while the true density was 0.995 g/cm³, and the corresponding porosity was 49.88%. The terminal velocity was 8.3 m/s. The static coefficient of friction on three different contacting materials has been found out and the results showed that the mean value of static coefficient friction was least in case of aluminum sheet while it is highest for plywood.     

Moisture-dependent physical properties of niger (Guizotia abyssinica Cass.) seed

Moisture-dependent physical properties of niger (Guizotia abyssinica Cass.) seed were studied at 5.60, 12.99, 19.77, 27.08 and 31.65% moisture content (wet basis). The length, width, thickness and geometric mean diameter increased significantly (p < 0.05) from 3.86 to 4.06 mm, 0.96 to 1.02 mm, 0.86 to 0.96 mm and 1.47 to 1.59 mm, respectively with increase in moisture content from 5.60 to 31.65% whereas the increase in sphericity from 38.10 to 39.01% was not significant. Similarly, thousand seed mass, porosity and angle of repose increased (p < 0.05) linearly from 2.50 to 3.69 g, 41.76 to 47.65% and 29.86° to 39.12°, respectively with increase in moisture content under the experimental condition. The bulk density decreased significantly (p < 0.05) from 635.23 to 561.06 kg m^?3 with increase in the moisture content range considered in the study, whereas the true density showed a slight increase from 1090.71 to 1098.42 kg m^?3 with increase in moisture content from 5.60 to 27.08% followed by a drop from 1098.42 to 1071.75 kg m^?3 as moisture content increased from 27.08 to 31.65%. Coefficient of static friction increased (p < 0.05) logarithmically from 0.34 to 0.51, 0.38 to 0.56 and 0.13 to 0.53 on mild steel, plywood and glass surfaces, respectively with increase in moisture content from 5.60 to 31.65%.     

Moisture-dependent physical properties of jatropha fruit

The physical properties of fruit are important in designing and fabricating equipment and structures for handling, transporting, processing and storage, and also for assessing quality. The study was conducted to investigate some physical properties of jatropha fruit at various moisture levels. The average length, width, thickness and 1000 mass were 29.31 mm, 22.18 mm, 21.36 mm and 1522.10 g, respectively, at moisture content of 7.97% d.b. The geometric mean diameter increased from 24.03 to 24.70 mm and the sphericity varied between 0.82 and 0.83 as moisture content increased from 7.97% to 23.33% d.b., respectively. In the same moisture range, the bulk and true densities decreased from 278 to 253 and 546 to 435 kg m^?3, respectively, whereas the corresponding porosity also decreased from 49.08% to 41.84%. As the moisture content increased from 7.97% to 23.33% d.b., crushing strength was decreased from 275 to 79 N, whereas the angle of repose and surface areas were found to increase from 36.41° to 41.72° and 1815.73 to 1917.59 mm², respectively. The static coefficient of friction of jatropha fruit increased linearly against the surfaces of three structural materials, namely plywood (47.81%), mild steel (62.88%) and aluminium (34.82%) as the moisture content increased from 7.97% to 23.33% d.b.     

Molybdenum-enriched soybean seeds enhance N accumulation, seed yield, and seed protein content in Brazil

High soybean yields require large amounts of nitrogen (N), which can be obtained mainly from symbiotic N₂ fixation. However, the efficiency of this biological process can be limited by micronutrient deficiencies, especially of molybdenum (Mo). In Brazil, soybean generally responds positively to fertilization with Mo in soils of low fertility and in fertile soils depleted of Mo due to long-term cropping. The micronutrient can be supplied by seed treatment, however toxicity of Mo sources to Bradyrhizobium strains applied to seed as inoculant has been observed, resulting in bacterial death and reductions in nodulation, N₂ fixation and grain yield. Therefore, use of seeds enriched in Mo could be a viable alternative to exterior seed treatment, allowing elite inoculant strains of Bradyrhizobium to sustain high rates of biological N₂ fixation. We demonstrated the feasibility of producing Mo-rich seeds of several soybean cultivars, by means of two foliar sprays of 400 g Mo ha⁻¹ each, between the R3 and R5 stages, with a minimum interval of 10 days between sprays. As a result of this method, considerable increases in seed-Mo content were obtained, of as much as 3000%, in comparison to seeds obtained from plants which received no Mo. In field experiments performed in soils with low N content and without any N-fertilizer supply, inoculation of Mo-rich seeds produced plants with increased N and Mo contents in the grain and higher yields of total N and of grain. In most cases, Mo-rich soybean seeds did not require any further application of Mo-fertilizer.     

Effects of plant density on the yield and yield components of true potato seed (TPS) hybrids in early and main crop potato production systems

This study was conducted to evaluate true potato seed (TPS) technology for use in ware or seed potato production in two contrasting environments in Turkey during 2002 and 2003. The field experiments were carried out in the Hatay and Nevsehir provinces in Turkey, which represent a Mediterranean early crop potato production area and a temperate main crop potato production area, respectively. The plug seedlings of six TPS hybrids were transplanted to the fields at four densities (15, 20, 25 or 30 plants m⁻²). The seed tubers of the medium early cultivar Marfona were also planted in the experimental plots to compare the performance of the TPS hybrids with traditional seed tubers. Transplanting of the seedlings was significantly delayed in Hatay due to unsuitable weather conditions in both years. The seedlings needed an adaptation period of 2–4 weeks after transplanting depending on the location and the growing conditions. The adaptation period was longer in Hatay due to high air temperatures after transplanting. Although the yield performance of the TPS hybrids differed depending on the location and year, the TPS hybrids produced noticeably higher total tuber yields in Nevsehir location (ranging from 43.1 to 62.5 t ha⁻¹ in 2002 and from 39.5 to 50.6 t ha⁻¹ in 2003) than in Hatay (ranging from 15.3 to 19.6 t ha⁻¹ in 2002 and from 15.1 to 19.1 t ha⁻¹ in 2003). The percentage of marketable tubers (>28 mm) was also considerably higher in Nevsehir. The optimal plant density varied between 25 and 30 plants m⁻² with regard to the total yield, while the optimal density with regard to the marketable yield was 20 or 25 plants m⁻² depending on hybrids in Nevsehir. However, none of the tested plant densities caused competition between plants in Hatay, where the environmental conditions during the growing period considerably restricted the growth of individual TPS seedlings.It was concluded that transplanting of TPS seedlings can be considered a feasible alternative for ware or seed potato production in temperate environments like Nevsehir that have growing periods of at least 4 months. However, there are several obstacles, such as difficulties with the timing of transplanting, long adaptation period that threaten the practicability of TPS technology in Mediterranean-type environments. Further agronomical studies focused on reducing inter- and intra-plant competition are needed for both environments in order to improve the acceptability of TPS technology to farmers.     

Mass transport studies of model migrants within dry foodstuffs

Cereals are widely consumed foodstuffs and it is therefore important to take them into account when estimating consumer exposure to packaging-related chemicals. The mass transport of three model migrants (diphenylbutadiene, triclosan and BHT) from low-density polyethylene (LDPE) within dry foodstuffs of different particle size (rice and wheat flour) was studied because of the relationship between consumer exposure and possible harmful effects on human health. The conditions that most affect the mass transport of substances within dry foods are evaluated and discussed. The diffusion coefficients (D_F) for diphenylbutadiene (DPBD) and triclosan in the studied foodstuffs were estimated. The D_F values for DPBD and triclosan in wheat flour at 25 °C were 7.1 × 10⁻⁸ cm²/s and 3.3 × 10⁻⁸ cm²/s, respectively. The D_F values for DPBD and triclosan in rice at 25 °C were 4.7 × 10⁻⁸ and 4.3 × 10⁻⁸ cm²/s, respectively. BHT displayed different behaviour and other tests were therefore carried out to elucidate the associated mass transport process.     

Method for analysis of fatty acid distribution and oil content on a single Lesquerella fendleri seed

Lesquerella fendleri is a developing oilseed crop suitable for temperate growing regions in the US. The seed oil is rich in lesquerolic acid (57%) and could serve as a domestic source of hydroxy fatty acids. A method for the analysis of fatty acids and total oil content of a half or single lesquerella seed was developed. Lesquerella seeds are small with 1000 seed weights around 0.6 g (half seed mass of 200–500 μg). Conventional analytical balances provide mass accuracy to ±40 μg which fails to provide sufficient accuracy/precession (4–40% mass error) for the initial seed mass. A microbalance which measures to ±2 μg provided good reproducibility in initial seed weights but was not suitable for mass balance of the extracted oil. A normal phase HPLC coupled to an evaporative light scattering detector gave good response for oil in the mass range of 22 μg to 110 μg/mL. Therefore, micelle concentrations from single or half seed extractions could be determined with good reproducibility. This method was then evaluated on bulk seed that had been fractionated on a gravity table. Gravity table fractionation of L. fendleri seeds obtained from a large field plot provided seven fractions with increasing bulk density. These fractions were then analyzed in sets of 30 individual seeds and as an aggregate of 50 seeds. Oil content for individual seeds varied widely (15.6–44.2%) as did lesquerolic acid content (42.2–63.7%). The mean oil content increased (27–33%) with increasing bulk density (684–745 g/L). The mean lesquerolic acid content did not correlate with bulk density.     

Efficacy of chloropicrin application by drip irrigation in controlling the soil-borne diseases of greenhouse pepper on commercial farms in Poland

Three demonstration experiments were conducted on commercial greenhouse farms to assess the efficacy of chloropicrin (CP), applied by drip irrigation, in controlling Verticillium wilt and root rot disease complex of bell pepper, in comparison with dazomet at 40 g m⁻². Chloropicrin was applied through drip irrigation system at 20, 30 and 40 g m⁻² of emulsified commercial formulation. The concentration of CP in water was constant, and the required doses were obtained by delivering different amounts of the irrigation water per area unit (from 12.5 to 33 mm). The highest mean efficacy in reducing the inoculum density of Verticillium dahliae in the soil at all locations was obtained after CP application at 30 and 40 g m⁻², about 85 and 86%, respectively. The number of viable microsclerotia recovered from the soil on the day of pepper planting was significantly correlated with the final incidence of Verticillium wilt disease (r = 0.962). The highest mean efficacy in controlling Verticillium wilt of pepper (86.4%) was obtained after soil treatment with CP at 40 g m⁻², and ranged from 80.2 to 95.6%. The yield was stronger correlated with root rot severity (r = −0.849**) than with progression of Verticillium wilt, expressed by AUDPC (r = −0.651**). The dominant soil-borne pathogen responsible for pepper root rot was Colletotrichum coccodes. All chemical treatments provided a significant reduction in root rot severity compared to the untreated control. On-thefarm evaluation revealed that soil fumigation with drip-applied chloropicrin presents a feasible option for pepper growers.     

Wheat grain tissue proportions in milling fractions using biochemical marker measurements: Application to different wheat cultivars

Measurement of biochemical markers allows the quantification of wheat (Triticum spp.) grain tissue proportions in milling fractions. In order to evaluate the ability of extending this methodology to an unknown wheat grain batch, the variability of the markers in the different tissues was assessed on various wheat cultivars. Ferulic acid trimer amounts in the outer pericarp ranged from 0.97 to 1.67 μg mg⁻¹ (dm) with an average value equal to 1.31 μg mg⁻¹ (dm). Alkylresorcinols amounts in a composite layer, including the testa, the inner pericarp and the nucellar epidermis, ranged from 10.5 to 16.7 mg g⁻¹ (dm), with an average value equal to 14.0 mg g⁻¹ (dm). In the aleurone layer, phytic acid amounts ranged from 94.9 to 187.2 mg g⁻¹ (dm) with an average value equal to 152 mg g⁻¹ (dm) whereas, para-coumaric acid ranged from 0.08 to 0.29 μg mg⁻¹ with an average level of 0.18 μg mg⁻¹. In the embryonic axis, wheat germ agglutinin ranged from 879 μg g⁻¹ to 2086 μg g⁻¹ with an average value of 1487 μg g⁻¹. The impact of this variability on tissue proportion determination was evaluated and a strategy to decrease the prediction error was suggested. Percentages of the outer pericarp, intermediate layer (including the testa), aleurone layer and embryonic axis within grains were calculated and their variability discussed.     

Forage soybean production for seed in mediterranean environments

Despite several experiments on row spacings and seeding rates of grain soybeans, limited information is available on the most suitable row spacing and seeding rate for tall and robust forage type soybeans grown for seed. The objectives of this study were to investigate seed yield, oil and protein content, and several morphological traits as affected by row spacing (20, 40, 60 and 80 cm) and seeding rate (330,000, 660,000, 990,000 and 1,320,000 seeds ha⁻¹) in tall and robust forage type soybeans in three irrigated Mediterranean environments in Turkey in a randomized split plot design with three replications in 2004 and 2005. Row spacings had no significant effect on plant height but tall and profusely branched plants developed in wide row spacing and light seeding conditions. Seed yield responded positively and linearly to row spacing up to 60 cm and then decreased slightly in all locations. Seed yield was the highest at 990,000 seeds ha⁻¹ seeding rate in all three locations (3072.5 kg ha⁻¹ in Bursa LSD = 214.7 kg ha⁻¹, 3295.1 kg ha⁻¹ in Mustafakemalpasa LSD = 298.6 kg ha⁻¹ and 3311.3 kg ha⁻¹ in Samsun LSD = 321.1 kg ha⁻¹). Averaged across years, locations, row spacings, and seeding rates the mean seed yield was an impressive 3013.4 kg ha⁻¹ compare with 3500.0 kg ha⁻¹ average seed yield of grain types. Crude protein and oil content of forage type soybean were not significantly affected by row spacings and seeding rates. It was concluded that forage type soybeans can be grown for multiple purposes at the 990,000 seeds ha⁻¹ seeding rate and 60 cm row spacings in Mediterranean environments.     

Comparative efficiency of chemical compounds for in vitro and in vivo activity against Clavibacter michiganensis subsp. michiganensis, the causal agent of tomato bacterial canker

Bacterial canker of tomato caused by Clavibacter michiganensis subsp. michiganensis produces considerable economic losses in many countries because effective control measures are lacking. The extent to which bactericides control this disease effectively is low and has not yet been well documented for Southern European conditions. In this study the bactericidal effect of several products on this pathogen was assessed in vitro and in vivo in tomato plants under greenhouse conditions. Seven antibacterial substances (bronopol, copper sulphate, kasugamycin, oxolinic acid, oxytetracycline, streptomycin and 8-hydroxy-quinoline), three commercial formulates (Antibak RZ, an oligoelements mixture containing copper plus zinc; Orthopol, a potassium soap; and Param, a resistance inductor) and combinations thereof were tested. In vitro assessment showed that minimal inhibitory concentration (MIC) of antibacterial substances was between 4–8 μg ml⁻¹, except for copper sulphate with a MIC value of 150 μg ml⁻¹ and kasugamycin, which was not active at 500 μg ml⁻¹. MIC values of commercial formulates ranged between 5 and 40 μl ml⁻¹. Furthermore, combinations of 8-hydroxy-quinoline+copper sulphate, 8-hydroxy-quinoline+Antibak RZ, streptomycin+Antibak RZ and streptomycin+Orthopol showed a synergistic effect at sub-inhibitory concentrations. Treatments containing copper sulphate greatly reduced disease symptoms on plants sprayed with the bacteria, whereas streptomycin was less effective. In two independent trials, the percentage of leaves showing symptoms was significantly lower (2.4% and 11.9%) after treatment with copper sulphate combined with 8-hydroxy-quinoline at half-dose, than in inoculated controls (75.1% and 59.6%). These results were better than copper sulphate alone. However, plants inoculated by pricking rapidly developed systemic infection, which no product managed to control significantly, although several treatments did reduce symptoms. We conclude that copper sulphate combined with 8-hydroxy-quinoline may be useful in controlling external symptoms of this disease in greenhouses, and is environmentally friendly, reducing the amount of copper applied to crops.     

Sensitivity of Sclerotinia sclerotiorum from oilseed crops to boscalid in Jiangsu Province of China

Between 2006 and 2008, 120 isolates of Sclerotinia sclerotiorum from the Jiangsu Province of China were characterized for the baseline sensitivity to boscalid, a new active ingredient that interferes with succinate ubiquinone reductase in the electron transport chain of fungi. The isolates collected between 2006 and 2008 all showed similar sensitivity to boscalid. Baseline sensitivities were distributed as unimodal curves with mean EC₅₀ values of 0.17 ± 0.09 μg ml⁻¹ for inhibition of mycelial growth. Inhibition of mycelial growth of isolates whether they had resistance to carbendazim or, dimethachlon or dual resistance to both carbendazim and dimethachlon was similar, suggesting that there was no cross-resistance between boscalid and MBC/dimethachlon already in commercial use. In field trials, the treatments boscalid (50% WG), at 125, 187.5 and 250 g ai ha⁻¹ provided higher control efficacies of 65.2, 69.0 and 75.1%, respectively, than the treatments carbendazim (50% WP), at 750 g ai ha⁻¹ and dimethachlon (46% WP), at 690 g ai ha⁻¹ with control efficacies of 18.9 and 44.6%, respectively.     

Alleviation of salinity stress on germination of Leymus chinensis seeds by plant growth regulators and nitrogenous compounds under contrasting light/dark conditions

The effects of plant growth regulators (PGRs) and nitrogenous compounds on alleviating salinity stress on seed germination of Leymus chinensis in light (12 h light/12 h dark) and dark (24 h dark) conditions were determined in a laboratory experiment. Seed germination was compared at various combinations of salinity and germination‐promoting compounds. Seed germination percentages were 57 and 74% under non‐saline conditions in light and dark, respectively, suggesting that germination was light‐inhibited. Germination decreased significantly with increasing salinity level in both light and dark conditions, and the reduction was greater in light than in dark at each salinity level. Seed germination percentage decreased to 22 and 7% in light but only to 51 and 27% in dark, in 100 and 200 mm NaCl solutions respectively. The influence of PGRs and nitrogenous compounds in alleviating salinity stress varied with light and salinity condition; e.g., gibberellins (GA_4 + 7) significantly increased germination percentages from 22%, 7% and 0·3% to 47%, 23% and 15% in light at 100, 200 and 300 mm NaCl, respectively, while they showed no effect on germination in darkness. In contrast, sodium nitroprusside and cytokinin significantly increased germination percentage in darkness at all salinity levels, but showed no effect on germination in light at 200 and 300 mm NaCl. Fluridone was very effective in alleviating salinity stress on germination in both light and dark; however, it was lethal to seedlings. Thiourea had no effect in alleviating salinity stress in either light or dark. Thus, alleviation of salinity stress on seed germination of L. chinensis by germination‐promoting compounds is strongly dependent on chemicals and light conditions.     

Effect of emergence time,inter- and intra-specific competition on growth and fecundity of Echinochloa crus-galli in dry-seeded rice

Since 2005, the evolution and spread of herbicide-resistant Echinochloa crus-galli biotypes have posed a serious threat to crop production in the Philippines. A comprehensive knowledge of E. crus-galli ecology and fecundity is fundamental in managing different biotypes of this weed. It was hypothesized that (a) high weed plant density produces more biomass and fertile seeds per unit area, (b) rice interference reduces the biomass and fecundity of the weed, and (c) a delay in weed emergence reduces the soil seed bank. In 2013, experiments were conducted in the wet season (WS) and dry season (DS), to understand the effect of E. crus-galli densities (40 and 80 plants m⁻²) on its growth, survival, and fecundity, with varying emergence times of 2, 15, 30, and 45 d after rice emergence (DARE). Relative to the weed plants grown without rice interference, E. crus-galli growth and seed production was lower in the presence of rice. Percent survival and plant height of E. crus-galli declined in a linear manner in the DS, and declined in a quadratic manner in the WS. Tiller number, inflorescence number, inflorescence biomass, and shoot biomass per plant declined in an exponential manner, with a delay in emergence of each cohort relative to rice. Across rice seeding rate, weed density, and emergence time, there was a linear relationship (y = 110x − 272 in the DS and y = 100x − 220 in the WS) between E. crus-galli shoot biomass and the number of seeds plant⁻¹. Relative to the late-emerging weed cohorts, E. crus-galli seed production (1320–1579 seeds plant⁻¹), 1000-seed weight (2.2–2.9 g), and seed yield (2808–2334 kg ha⁻¹) were higher when seedlings emerged with the crop (2 DARE). None of the seedlings that emerged 45 DARE produced viable seeds. Seed germination of the first two cohorts (2 and 15 DARE) ranged from 84 to 91%. The delay in emergence of E. crus-galli beyond 30 DARE reduced the percentage of germinable and viable seeds, and increased the percentage of non-viable seeds produced plant⁻¹. The results suggest that cultural weed management approaches that delay the emergence of E. crus-galli can reduce weed biomass and seed production, and is thus valuable for preventing seed rain to the seed bank by noxious weed biotypes in the field.     

Fungicidal activity of fluopicolide for suppression of Phytophthora capsici on squash

The efficacy of a new fungicide fluopicolide in suppression of Phytophthora blight caused by Phytophthora capsici was evaluated under laboratory and field conditions. Studies with 51 P. capsici isolates from vegetable crops in Georgia, USA, indicated that 5.9% of the isolates were resistant, 19.6% were intermediately sensitive, and 74.5% were sensitive to 100 μg ml⁻¹ of mefenoxam based on in vitro mycelial growth. EC₅₀ values of fluopicolide in inhibiting mycelial growth of 25 isolates, representing resistant, intermediately sensitive, and sensitive to mefenoxam, ranged from 0.05 to 0.35 μg ml⁻¹ with an average of 0.2 μg ml⁻¹ EC₅₀ values of fluopicolide in suppressing zoospore germination and sporangium production of the 25 isolates ranged from 1.1 to 4.5 μg ml⁻¹ and 0.3–9.0 μg ml⁻¹, respectively. Evaluation of a collection of 150 P. capsici isolates from vegetables and irrigation ponds found none of the isolates were resistant to 10 μg ml⁻¹ of fluopicolide. Field experiments were conducted to determine the efficacy and application methods of fluopicolide for control of P. capsici on squash in spring 2007 and 2009. Fluopicolide applied through drip irrigation or as a foliar spray at 86.6 or 115.4 g ha⁻¹ consistently provided significant disease reduction and increased squash yield. Results with fluopicolide were similar or slightly superior to mefenoxam applied at recommended rate. Fluopicolide applied at 57.7 g ha⁻¹ did not provide consistent satisfactory disease suppression. The results indicated that fluopicolide was effective in suppression of different stages of the life cycle of P. capsici and could be a viable alternative to mefenoxam for managing Phytophthora blight in squash production.     

Yield formation of CO2-enriched hybrid rice cultivar Shanyou 63 under fully open-air field conditions

Hybrid indica rice (Oryza sativa L.) cultivars play an important role in rice production system due to its heterosis, resistance to environmental stress, large panicle and high yield potential. However, no attention has been given to its yield responses to rising atmospheric [CO₂] in conjunction with nitrogen (N) availability. Therefore we conducted a free air CO₂ enrichment (FACE) experiment at Yangzhou, Jiangsu, China (119°42′0′′E, 32°35′5′′N), in 2004–2006. A three-line hybrid indica rice cv. Shanyou 63 was grown at ambient and elevated (ca. 570 μmol mol⁻¹) [CO₂] under two levels of supplemental N (12.5 g Nm⁻² and 25 g Nm⁻²). Elevated [CO₂] had no effect on phenology, but substantially enhanced grain yield (+34%). The magnitude of yield response to [CO₂] was independent of N fertilization, but varied among different years. On average, elevated [CO₂] increased the panicle number per square meter by 10%, due to an increase in maximum tiller number under enrich [CO₂], while productive tiller ratio remained unaffected. Spikelet number per panicle also showed an average increase of 10% due to elevated [CO₂], which was supported by increased plant height and stem dry weight per tiller. Meanwhile, elevated [CO₂] caused a significant enhancement in both filled spikelet percentage (+5%) and individual grain weight (+4%). Compared with the two prior FACE studies on rice, hybrid indica rice cultivar appears to profit much more from elevated [CO₂] than japonica rice cultivar (ca. +13%), not only due to its stronger sink generation, but also enhanced capacity to utilize the carbon sources in a high [CO₂] environment. The above data has significant implication with respect to N strategies and cultivar selection under projected future [CO₂] levels.