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哺乳母兔感染大肠埃希氏杆菌的诊断戴惠德(新疆兵团农一师畜牧兽医工作站,阿克苏843000)笔者在80年代曾发现某实验兔场中的哺乳母兔,突然暴发疑似某种传染病的急性死亡病例,病程一般为1~2天或稍长,1周内急性死亡12只(其中笼养9只,厩养3只),当时... 相似文献
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鸡大肠埃希氏杆菌灭活苗的研制和应用 总被引:1,自引:0,他引:1
鸡大肠埃希氏杆菌灭活苗的研制和应用李超(山东省莒南县畜牧局)鸡大肠杆菌病是由大肠埃希氏菌的某些血清型引起的一类疾病的总称。大肠杆菌有许多不同的血清型,到目前为止共分离到O抗原154个,K抗原89个,H抗原49个,与禽病有关的血清型主要是O-1、O-2... 相似文献
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大肠埃希氏菌通常被称作大肠杆菌,外文名称(学名)Escherichia coli。它属于细菌界,变形菌门,V-变形菌纲,肠杆菌目,肠杆菌科,埃希氏菌属,大肠杆菌种。大肠杆菌主要寄生于人和动物的大肠内,约占肠道菌的1%。是一种两端钝圆、能运动、无芽孢的革兰氏阴性短杆菌。该菌常引起动物严重腹泻和败血症。 相似文献
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2005年4~10月,广东省湛江市某养殖场的小尾寒羊出现一种以极度贫血、衰弱、消瘦为主要症状的疾病。笔者通过流行病学调查、病理剖检、血液检验、寄生虫体鉴定及微生物学诊断,确诊为扩展莫尼茨绦虫(M.Expansa)及大肠埃希氏杆菌(E.coli)混合感染。经采取丙硫咪唑、环丙沙星治疗等综合防制措施后,病情得到了有效控制。 相似文献
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《养禽与禽病防治》2008,(11)
为了有效防控鸡源性大肠埃希氏菌病、指导科学用药,本站从2005年11月至2007年5月对商丘市129个发病鸡场送检的病料进行了大肠杆菌分离鉴定、药敏试验和血清型鉴定。结果发现本地区出现了大量耐药的菌株,通过药敏试验筛选出的敏感药物及其有效率如下:壮观霉素75%、阿米卡星73%、氨苄西林(舒巴坦钠)65%、头孢噻肟钠50%、磷霉素45%、庆大霉素41%、新霉素41%。血清型鉴定结果发现O78、O15两个血清型为本地区的优势血清型,另本地区还存在O1、O2、O5、O18、O35、O50、O86、O88、O111等多种血清型。本调查研究的结果,对指导本地区乃至其他地区鸡源大肠杆菌病的防治有重要的参考价值。 相似文献
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腹泻犊牛大肠埃希菌的分离鉴定及药敏试验 总被引:1,自引:0,他引:1
对陕西省某奶牛场一起犊牛腹泻病例进行诊断和治疗。无菌采集腹泻犊牛肝脏和脾脏组织进行微生物学检测、小鼠致病性试验和药敏试验。结果显示,被检测病料中,牛病毒性腹泻病毒(BVDV)、牛腺病毒7型(BAdV-7)、牛冠状病毒(BCoV)、牛轮状病毒(BRV)检测结果均为阴性;分别从脾脏和肝脏组织分离培养到2株细菌,其培养特性和生化试验结果与大肠埃希菌高度相似,16S rRNA基因测序结果与大肠埃希菌的同源性为100%;动物致病性试验显示,分离菌能致试验小鼠死亡;药敏试验结果显示,该菌对左氧氟沙星、庆大霉素高度敏感,对头孢呋辛、恩诺沙星不敏感;临床选择敏感药物左氧氟沙星进行治疗,犊牛腹泻很快得到控制。 相似文献
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腹泻犊牛大肠杆菌的分离鉴定和药敏试验 总被引:2,自引:0,他引:2
犊牛腹泻是犊牛常见病之一.犊牛腹泻大肠杆菌有多种血清型,主要有导致初生犊牛腹泻的O8、O9、O20、O101大肠杆菌,感染犊牛发生败血症的O78、O15、O35、O115、O117、O137等血清型大肠杆菌[1].2005~2007年我们从南京地区部分奶牛场犊牛腹泻和犊牛败血症病例的犊牛,无菌采集直肠棉拭及病死犊牛的脾脏和关节液作病料,同时无菌采集部分临床健康的犊牛和成年奶牛直肠棉拭,划线于麦康凯平板,37℃培养24 h; 相似文献
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自永定肉鸭养殖场分离到17株大肠杆菌,对其进行了常用抗生素敏感性测定。结果显示所检测的25种抗生素中,无高敏药,中敏药物仅为阿米卡星、头孢类药物。由此表明鸭大肠杆菌分离菌株的耐药性在不断增强、耐药谱在拓宽。 相似文献
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Twenty-one isolates of Escherichia coli recovered from chickens and turkeys were evaluated for pathogenicity in 1-week-old chicks. Fifteen produced coli-septicemia (pathogenic) and six were innocuous (nonpathogenic). Both pathogenic and nonpathogenic E. coli were tested for their ability to selectively absorb Congo red (CR) dye incorporated into agar medium. Eight of 15 pathogenic E. coli (somatic antigen types O1, O78, O11, O88, and OX9) absorbed the dye and produced red colonies (CR+) between 48 to 72 hours of incubation. All serotypes of E. coli with homologous somatic antigen O78 were CR+, while those of O2 antigen were CR- (white colonies). Five of six nonpathogenic E. coli also were CR+. In contrast to pathogenic E. coli, however, nonpathogenic isolates absorbed CR early, between 18 to 24 hours of incubation. Although CR dye binding did not correlate well with pathogenicity, it may be an identifiable property of some serotypes of E. coli. 相似文献
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近年来,肉类生产经营渠道增多,货源复杂.上市肉品有的经过严格的屠宰检疫检验,有的未经过任何检查,甚至有些业主或商贩非法收购病死动物,加工后上市出售.为防止未经过检疫肉品上市交易,保障人民身体健康,必须搞好上市肉品的监督检查工作,尤其要防范和杜绝病死畜禽肉上市. 相似文献
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Virulence-associated genes in Escherichia coli isolates from poultry with colibacillosis 总被引:1,自引:0,他引:1
Avian pathogenic Escherichia coli, the causative agent of colibacillosis, harbors several putative virulence genes. In this study we examined by polymerase chain reaction (PCR) the presence of 16 of those genes in 200 colibacillosis isolates from our region. The seven virulence genes iutA, iss, cvaC, tsh, papC, papG and felA were detected significantly more often amongst colibacillosis isolates than in fecal isolates from healthy birds, thereby confirming their worldwide occurrence and possible pathogenic role in colibacillosis. However, several of those genes were not detected in many colibacillosis isolates, and none of them were detected in 27.5% of those isolates, which suggests that variants of those genes and yet undetected virulence factors should be searched for. 相似文献
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Colibacillosis accounts for significant losses to the poultry industry, and control efforts are hampered by limited understanding of the mechanisms used by avian pathogenic Escherichia coli (APEC) to cause disease. We have found that the presence of the increased serum survival gene (iss) is strongly associated with APEC but not with commensal E. coli, making iss, and the protein it encodes (Iss), candidate targets of colibacillosis control procedures. To assess the potential of Iss to elicit a protective response in chickens against APEC challenge, Iss fusion proteins were produced and administered subcutaneously to four groups of 2-wk-old specific-pathogen-free leghorn chickens. At 4 wk postimmunization, birds were challenged with APEC from serogroups 02 and 078 via intramuscular injection. At 2 wk postchallenge, birds were necropsied, and lesions consistent with colibacillosis were scored. Also, sera were collected from the birds pre- and postimmunization, and antibody titers to Iss were determined. Immunized birds produced a humoral response to Iss, and they had significantly lower lesion scores than the unimmunized control birds following challenge with both APEC strains. Birds that received the smallest amount of immunogen had the lowest lesion scores. Although further study will be needed to confirm the value of Iss as an immunoprotective antigen, these preliminary data suggest that Iss may have the potential to elicit significant protection in birds against heterologous E. coli challenge. 相似文献