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1.
Evaluation of antibacterial activity and innate immune components in skin mucus of Indian major carp,Cirrhinus mrigala
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Ashwini Kumar Nigam Usha Kumari Swati Mittal Ajay Kumar Mittal 《Aquaculture Research》2017,48(2):407-418
The present work has been undertaken to analyse the antibacterial activity and innate immune components in the skin mucus of Indian major carp, Cirrhinus mrigala. Skin mucus was extracted separately in triple‐distilled water (TDW), 3% acetic acid (3% AA) or 0.1% trifluoroacetic acid (1% TFA). All mucus extracts exhibited different spectrum of the antibacterial activity against different groups of pathogenic bacteria. Protein profiling by polyacrylamide gel electrophoresis revealed a series of protein bands in the TDW extract, four major protein bands in the AA extract and two protein bands in the TFA extract. Tandem mass spectrometry analysis of distinct protein bands identified potential innate immune factors – histone H2A, histone H3, histone H4, haemoglobin, cofilin and nucleoside diphosphate kinase in the TDW extract, and ubiquitin and histone H2B isoforms in acidic extracts of skin mucus of C. mrigala. The presence of these innate immune molecules suggests that skin mucus play an important role in the protection of the fish against microbial invasion. 相似文献
2.
麦瑞加拉鲮鱼的人工繁殖技术 总被引:2,自引:0,他引:2
麦瑞加拉鲮鱼(Cirrhinusmrigala)为杂食性底层鱼类,生长快,个体较大,病害少。目前已在广东及华南一些地区推广养殖,其成鱼养殖面积正逐年增加。我们经过几年的试验和改进,池养麦瑞加拉鲮鱼的人工催产技术已趋完善,现报告如下。1亲鱼培育亲鱼培育... 相似文献
3.
Manoharan Saravanan Mathan Ramesh Rakpong Petkam 《Fish physiology and biochemistry》2013,39(6):1431-1440
The extensive use of pharmaceuticals in human and veterinary medicine may enter the aquatic environment and pose a serious threat to non-target aquatic organisms like fish. In this study, Indian major carp Cirrhinus mrigala was exposed to different concentrations (1, 10 and 100 μg L?l) of most commonly used pharmaceutical drugs clofibric acid (CA) and diclofenac (DCF) to evaluate its impacts on certain enzymological parameters during short- and long-term exposures. During short-term (96 h) exposure period, plasma glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) and gill Na+/K+-ATPase activity were significantly altered at all concentrations of both the CA- and DCF-treated fish. In long-term exposure (35 days), gill Na+/K+-ATPase activity was found to be significantly increased at all concentration of CA and DCF exposures throughout the study period (except at the end of 7th day in 10 and 100 µg L-1) . However, a biphasic trend was observed in plasma GOT and GPT activity when compared to the control groups. In both short- and long-term exposure, a significant (P < 0.01 and P < 0.05) changes were observed in all enzymological parameters of fish C. mrigala exposed to different concentrations of CA and DCF. The alterations of these enzymological parameters can be effectively used as potential biomarkers in monitoring of pharmaceutical toxicity in aquatic environment and organisms. 相似文献
4.
5.
Parishmita Handique Hemanta Pokhrel Binod Kalita Raktim Sarmah Abdul Malik Ahmed Kaustabh Bhagawati Arnab Narayan Patowary 《Aquaculture Research》2020,51(5):1973-1979
The present study was conducted to evaluate the …effect of dietary selenium on growth performance and antioxidant enzymes activity in Cirrhinus mrigala for a period of 45 days. Four numbers of experimental diets were prepared, Tc for Control (0 mg organic selenium/kg of diet), T1 (2.5 mg organic selenium/kg of diet), T2 (5.0 mg organic selenium/kg of diet) and T3 (10 mg organic selenium/kg of diet). The water quality parameters (temperature, DO, pH, conductivity, hardness, ammonia, nitrate and nitrite) were found to be insignificant (p > .05) among all the treatments. Superoxidase dismutase (SOD) was found to be significantly highest in T2 (6.10 ± 0.07) when compared with the other treatments. Similarly, catalase (CAT) activity in T1 (13.21 ± 0.13) was significantly (p < .05) different with that of Control, T2 and T3. Present study also revealed that 2.5mg organic selenium/kg supplemented diet (T1) showed highest results in net weight gain (15.55 ± 1.36 g), net weight gain percentage (185.88 ± 8.98%) and SGR (2.35 ± 0.06 g) compared with other experimental diet. 相似文献
6.
Digestive enzymes of Cirrhinus mrigala (Ham.) were studied during ontogenic development. Specific amylase activity was detected in first feeding fish. The enzyme activity decreased up to day‐18 and then it increased with the age of fish to reach the highest level on day‐34. Protease activity was 28.61 ± 8.90 mU mg protein?1 min?1 on day‐4 and increased with the age throughout the study period. Trypsin activity was 31.86 ± 1.12 mU mg protein?1 min?1 on day‐4. The activity decreased up to day‐10 and from day‐12 onwards increased up to day‐26. Chymotrypsin activity was 14.56 ± 2.74 mU mg protein?1 min?1on day‐4 and constantly increased up to day‐26. A significant increase in lipase activity was observed between days‐24 and 34. SDS‐PAGE and substrate SDS‐PAGE showed the diversity of protein (17.4–127.8 kDa) and protease activity bands (16.6–88.8 kDa) during ontogenesis. Soybean trypsin inhibitor, phenyl methyl sulphonyl fluoride, N‐α‐p‐tosyl‐l ‐lysine chloromethylketone and N‐tosyl‐l ‐phenylalanine chloromethylketone inhibited the protease activity up to 79.72–97.21, 65.55–94.83, 45.41–75.31 and 40.78–64.72%, respectively. Inhibition study in substrate SDS‐PAGE revealed the abundance of serine proteases and the presence of isoforms of trypsin and chymotrypsin. Ethylenediamine‐tetraacetate showed 5.56–22.78% inhibition of metal ion‐specific enzyme activity. 相似文献
7.
Dietary thiamin and pyridoxine requirements of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)
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Two experiments were conducted to quantify the dietary thiamin (experiment I) and pyridoxine (experiment II) requirements of fingerling Cirrhinus mrigala for 16 weeks. In experiment I, dietary thiamin requirement was determined by feeding seven casein–gelatin‐based diets (400 g kg?1 CP; 18.69 kJ g?1 GE) with graded levels of thiamin (0, 0.5, 1, 2, 4, 8 and 16 mg kg?1 diet) to triplicate groups of fish (6.15 ± 0.37 cm; 1.89 ± 0.12 g). Fish fed diet with 2 mg kg?1 thiamin had highest specific growth rate (SGR), protein retention (PR), RNA/DNA ratio, haemoglobin (Hb), haematocrit (Hct), RBCs and best feed conversion ratio (FCR). However, highest liver thiamin concentration was recorded in fish fed 4 mg thiamin kg?1 diet. Broken‐line analysis of SGR, PR and liver thiamin concentrations exhibited the thiamin requirement in the range of 1.79–3.34 mg kg?1 diet (0.096–0.179 μg thiamin kJ?1 gross energy). In experiment II, six casein–gelatin‐based diets (400 g kg?1 CP; 18.69 kJ g?1 GE) containing graded levels of pyridoxine (0, 2, 4, 6, 8 and 10 mg kg?1 diet) were fed to triplicate groups of fish (6.35 ± 0.37 cm; 1.97 ± 0.12 g). Fish fed diet containing 6 mg kg?1 pyridoxine showed best SGR, FCR, PR, RNA/DNA ratio, Hb, Hct and RBCs, whereas maximum liver pyridoxine concentration was recorded in fish fed 8 mg kg?1 dietary pyridoxine. Broken‐line analysis of SGR, PR and liver pyridoxine concentrations reflected the pyridoxine requirement from 5.63 to 8.61 mg kg?1 diet. Data generated during this study would be useful in formulating thiamin‐ and pyridoxine‐balanced feeds for the intensive culture of this fish. 相似文献
8.
Identification and partial characterization of potential probiotic lactic acid bacteria in freshwater Labeo rohita and Cirrhinus mrigala
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Muhammad Shahid Bilal Hussain Danish Riaz Mohsin Khurshid Muhammad Ismail Mohsin Tariq 《Aquaculture Research》2017,48(4):1688-1698
Carps are the most diversified freshwater fish belonging to family Cyprinidae. Numerous probiotic and pathogenic lactic acid bacteria (LAB) have been characterized from carps. However, the diversity of these ecologically important bacteria is entirely unknown in freshwater fish of Pakistan. The present study aimed to characterize and identify the lactic acid bacteria from two carps viz. Laboe rohita and Cirrhinus mrigala and determine their antagonistic activity. Seventeen bacterial isolates were purified from the gastrointestinal tract and gills of these fish and characterized morphologically. Initially, seven isolates were screened as LAB using agar supplemented with CaCO3. Subsequently, only two isolates CILB2 and RIL10 were selected as LAB after high‐performance liquid chromatography analysis for lactic acid production. Isolates CILB2 and RIL10 were genetically identified as Enterococcus faecalis and Weissella sp., respectively after 16S rRNA gene sequence analysis. Both strains exhibited significant antagonistic activity against common fish pathogens Streptococcus agalactiae, Staphylococcus aureus and Escherichia coli. Enterococcus faecalis CILB2 and Weissella sp. RIL10 were also found negative for haemolysis and gelatinase activities and were sensitive to ampicillin, amoxicillin, doxycycline, erythromycin, chloramphenicol and co‐trimoxazole antibiotics. The identified LAB strains may further be investigated for their potential probiotic application in fish feed and food preservation techniques. 相似文献
9.
Acute toxicity of ammonia and its sub-lethal effects on selected haematological and enzymatic parameters of mrigal, Cirrhinus mrigala (Hamilton) 总被引:2,自引:0,他引:2
A comprehensive acute toxicity trial was conducted using a static water system to study the toxic effect of ammonia on haematology and enzyme profiles of Cirrhinus mrigala H. The LC50 of total ammonia‐nitrogen (TAN) was 11.8 mg L?1 TAN (1.029 mg L?1 NH3‐N). The sub‐lethal test revealed that with increasing concentration of TAN, the total erythrocyte counts were reduced in lower concentrations (1–4 mg L?1 TAN) followed by higher levels in fish exposed to higher concentrations (8–16 mg L?1 TAN). In contrast, the total leucocyte counts were opposite. With increasing concentration of TAN, haemoglobin and serum protein content were reduced, whereas the blood glucose level increased. As the concentration of ammonia increased, there was a reduction in acetylecholinesterase activity in the brain and liver; alkaline phosphatase activity in the serum, brain and gill; and acid phosphatase (ACP) activity in the gill. The activity of lactate dehydrogenase in the gill, liver, kidney and brain increased with increased concentration of ammonia. In addition, activities of ACP in the serum and brain, alanine aminotransferase in the serum, brain and gill, and aspartate aminotransferase in the serum, brain and gill were increased. 相似文献
10.
Heat shock protein 70 expression in different tissues of Cirrhinus mrigala (Ham.) following heat stress 总被引:2,自引:0,他引:2
Members of heat shock protein 70 (HSP70) are highly conserved proteins of about 70 kDa and play important roles in protein folding. Levels of these proteins increase when cells are under stress. Environmental temperature influences both the basal and induced levels of HSPs. However, studies on HSPs in fishes from a tropical country such as India are lacking. In the present study, Indian major carp (IMC) Cirrhinus mrigala (Ham.) acclimatized at 25±2°C had high levels of HSP70, viz., 1.2–1.3 ng μg?1 total protein in kidney and gill and 4.2–5.3 ng μg?1 total protein in liver and brain tissues, indicating the presence of biochemically significant levels of stress. However, maintenance of acclimatized fish at 17°C for up to 48 h did not lead to a significant decrease in stress protein levels. A heat shock at 37°C for up to 48 h resulted in only two to threefold increase in HSP70 levels in these organs. Although the increase in HSP70 levels was apparent from the first hour of heat stress in all these tissues, the increase was significant from the second hour in the brain, the sixth hour in liver and kidney and the 20th hour in the gills. 相似文献
11.
A polyculture experiment with the large carp rohu, catla and either mrigal or common carp (as cash crop fish), and the small indigenous fish punti (as food for the farmer's family) was carried out at Bangladesh Agricultural University, Mymensingh. The main objectives were to compare polycultures of large carp in which the bottom feeder is either the native mrigal or the exotic common carp, and to assess the effects of adding the small indigenous species punti to those polycultures. The results of fish–fish interactions and overall fish production have already been reported. The present paper presents the effects on the water quality, and discusses fish–environment interactions. The main conclusions are: time changes in the pond environment were stronger than fish composition effects. The main practice affecting water quality was liming, that incresed alkalinity, pH and water transparency and decreased ammonia. Rain affected photosynthesis and the match‐mismatch of the two steps of nitrification. The more that bottom feeding fish species disrupt the mud bottom, the stronger their effects on pond environment. Common carp produce the strongest disruption of the mud bottom, followed by punti and then by mrigal. Mud disruption produced by common carp leads to a stronger liming effect, nutrient release into the water, and provides more particles that rain‐floods wash out, facilitating the mismatch of the two steps of nitrification, and increased phosphorus adsorption into the mud bottom. Mud disruption by punti is only enough to improve the liming effect. Mud disruption by mrigal is the least, hence less particles are resuspended, nitrification is not affected during floods and relatively more phosphate remains in the water available for photosynthesis. The bottom feeder common carp can be seen not only as a target‐cultured fish but also as a management tool. Farmers can get double benefit in introducing common carp in the ponds as it enhances the effectiveness of lime application and increases the availability of nutrients to phytoplankton. Through the manipulation of species in the polyculture alone, farmers can maintain the environment better and also reduce input costs. 相似文献
12.
Amul Sakharkar Praful Singru Nishikant Subhedar 《Fish physiology and biochemistry》2005,31(2-3):163-166
We studied GnRH immunoreactivity in the pineal gland of the Indian major carp Cirrhinus mrigala during different phases of reproductive cycle. In the resting phase (December–January), GnRH immunoreactive (-ir) fibers
were organized as paired fascicles above the posterior commissure that ascend in the stalk and distribute widely in the pineal
gland. The GnRH-ir fiber density significantly declined (P<0.001) during the preparatory phase (February–April) and the fibers disappeared thereafter. While no GnRH fibers were seen
during the prespawning (May–June) and spawning (July–August), isolated GnRH-ir fibers reappeared in the postspawning phase.
Since no GnRH cell bodies were detected in the pineal, these GnRH-ir fibers seem to be of central origin. The results reveal
a distinct reciprocal relationship between the GnRH immunoreactivity in the pineal and the status of the ovarian maturity;
the fibers appeared in the pineal only during the period of ovarian quiescence. While the functional significance of these
cyclic changes in GnRH is yet to be determined, we suggest that the decapeptide may serve as a transmitter of central origin
that modulates the activity of the pineal gland. 相似文献
13.
Four different fertilization frequencies, namely twice per week, once per week, twice a month and once a month, were used in ponds to assess their effects on nutrient release, pond productivity and fish biomass. All ponds received the same total fertilizer inputs during the experimental period of 60 days (cow dung 208.3 kg ha?1 week?1, TSP 9.8 kg ha?1 week?1, urea 6.0 kg ha?1 week?1). Studies have revealed that the highest values of fish biomass, specific growth rate (SGR), net primary productivity (NPP), plankton population and nutrients were observed in the ponds that were fertilized twice a month. A strong and significant correlation of fertilization frequency was observed with dissolved oxygen, biochemical oxygen demand (BOD), alkalinity, nutrient release, NPP, plankton density (no. L?1), fish biomass and SGR. The linear relationship between NPP and fish biomass/SGR for all the ponds was strong (r2= 0.88). Sediment chemistry revealed that O‐PO4, NO3‐N, organic carbon and electrical conductivity (EC) increased significantly (P<0.05) with a decrease in the frequency of fertilization, while alkalinity and calcium were high in ponds that were fertilized twice a month. 相似文献
14.
A polyculture experiment with the large carp rohu, Labeo rohita (Hamilton), catla, Catla catla (Hamilton) and either mrigal, Cirrhinus mrigala (Hamilton) or common carp, Cyprinus carpio (L.) (as cash crop fish), and the small indigenous fish punti, Puntius sophore (Hamilton) (as food for the small‐scale farmer family) was carried out at the Field Laboratory of the Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. The main objective was to compare polycultures of large carp in which the bottom feeder is either the native mrigal or the exotic common carp. Secondary objectives were to assess the effects of adding the small indigenous species punti to polycultures of large carp, and to compare the effects of mrigal and common carp on punti production and reproduction. It was found that (i) common carp damaged embankments, had no effect on catla, improved rohu performance by 50% and total fish production by 20%; (ii) punti addition did not affect rohu, catla and total yield, improved mrigal performance by 50%, and decreased common carp performance by 20%; and (iii) punti was not affected either by common carp or by mrigal. However, its performance was not satisfactory, probably owing to frequent netting, which might have hindered growth and breeding. In spite of the embankment damage caused by common carp, this bottom feeder seems to be more promising than mrigal, because it leads to higher fish production. The addition of punti to the large carp polyculture is a viable proposition, as it does not reduce cash crop production, and might be a good food source for a small‐scale farmer's family. 相似文献
15.
S. JOSE M.V. MOHAN S. SHYAMA K.G. RAMACHANDRAN NAIR & P.T. MATHEW 《Aquaculture Nutrition》2006,12(4):275-279
The effect of varying dietary levels of defatted soybean meal on the growth and survival of mrigal, Cirrhinus mrigala (Hamilton) was investigated. In a feeding trial of 90 days, three experimental diets containing soybean meal at 200, 300 and 400 g kg?1 level of incorporation were fed to quadruplicate groups of 10 fish each. The conventional feed used in India, consisting of a mixture of groundnut oil cake and rice bran in 1 : 1 ratio served as the control. Best growth in terms of percentage weight gain, specific growth rate, protein efficiency ratio (PER), feed conversion ratio and survival rate was obtained for the test diet with 354 g kg?1 crude protein and with 400 g kg?1 soybean meal inclusion level. However, no statistical significant difference was observed between the three soybean‐based diets, except for PER and survival rate. Soybean meal is an easily available, acceptable and cost‐effective protein source in formulated feeds for Indian major carps. The results of the present study indicate that a diet of 350 g kg?1 overall protein with soybean meal included at 400 g kg?1 can elicit good growth response and survival in mrigal. 相似文献
16.
Optimum ration size of Indian major carp, Cirrhinus mrigala fingerlings was determined by feeding purified diet (40% crude protein (CP); 3.61 kcal g?1) at five ration sizes (2%, 4%, 6%, 8% and 10% of body weight per day) for 6 weeks. Feeding trial was conducted in triplicate. Fishes were randomly stocked at the rate of 20 fish per trough fitted with water flow‐through system. The best feed conversion ratio (FCR), specific growth rate (%) and protein efficiency ratio (PER) were evident at the ration size of 4–6% body weight. Second‐degree polynomial regression analysis of the FCR, PER, and protein and energy retention data indicated the breakpoints at ration size of 5.16%, 5.24%, 5.52% and 5.42% body weight per day. Carcass composition of fish fed different ration sizes varied significantly. Maximum carcass protein and minimum moisture content were noticed at 4% and 6% ration levels. A linear increase in fat content was evident with increasing ration levels up to 6% body weight. Ash content remained insignificantly different among various ration levels except at 2%, showing the significantly highest value. Water temperature, dissolved oxygen, free carbon dioxide, pH and total alkalinity were recorded regularly during the length of the experiment. No mortality was observed during the feeding trial. Based on the above results, it is recommended that feeding in the range of 5–5.5% body weight per day corresponding to 20 g protein and 181 kcal energy to 22 g protein and 199 kcal energy per kg of the diet per day is optimum for the growth and efficient feed utilization of C. mrigala. 相似文献
17.
Effect of dietary synbiotic on growth performance,body composition,digestive enzyme activity and gut microbiota in Cirrhinus mrigala (Ham.) fingerlings
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A feeding trial of 60 days was conducted to delineate the effect of dietary synbiotic on maximum growth, body composition, digestive enzyme activity and subsequently gut microbiota in Cirrhinus mrigala fingerlings. One hundred and eighty acclimatized fingerlings of mrigal with initial body weight ranging from 2.87 ± 0.01 g to 3.26 ± 0.05 g were randomly distributed in three replicates of each of four experimental groups including control (without probiotic and prebiotic), T1 (high probiotic + low prebiotic), T2 (low probiotic + high prebiotic) and T3 (high probiotic + high prebiotic), using completely randomized design (CRD). Results showed that growth performance parameters, such as specific growth rate (SGR), per cent weight gain, feed conversion ratio (FCR) and protein efficiency ratio (PER), were reported to be higher in the T2 group followed by the T3 group. Maximum gut microbiota activity was found in the T3 group which was significantly different from other treatment groups. Similarly, body composition and digestive enzyme activity varied significantly (p < .05) among the treatment groups. The study showed the possibility of improved nutrient utilization in terms of growth performance and digestive enzyme activity in the group following dietary synbiotic supplementation. 相似文献
18.
Abstract.— The effects of phytic acid on growth, protein efficiency, feed conversion, and carcass composition of mrigal Cirrhinus mrigala fry (2.5–3.5 cm) reared indoors at 18–22 C in 70-L flow-through (1-1.5 L/min) circular tanks were examined. Fish were fed isonitrogenous (40% crude protein) and isocaloric (4.32 kcal/g) purified test diets in the form of moist cake containing different levels (0.5, 1.0, 1.5, 2.0 and 2.5%) of phytic acid (dodecasodiurn salt) at a rate of 4% body weight twice daily (0800 and 1600 h). The highest weight gain (94.87%). specific growth rate (133%). protein efficiency ratio (2.02), and best feed conversion ratio (1.21) were observed in fish fed the control diet containing no phytic acid. Live weight gain and specific growth rate were significantly reduced by dietary phytic acid inclusion above 1%. Dietary inclusion of phytic acid markedly influenced the carcass composition of the fish. Whole body crude protein and fat content declined significantly ( P < 0.05) in fish fed diets containing phytic acid, while percentage of moisture and ash in these fish was significantly ( P ≤ 0.05) higher than fish in control diets. 相似文献
19.
Ravendra Kumar Singh Ajay S. Desai Sugandha L. Chavan Punam A. Khandagale 《Journal of the World Aquaculture Society》2008,39(2):275-280
Experiment was conducted to ascertain the effect of l ‐carnitine on growth and body composition of Cirrhinus mrigala fry (0.342 ± 0.03 g) using five different concentrations (0.25, 0.50, 0.75, 1, and 0%) of l ‐carnitine‐incorporated diets for 120 d. At the end of trial, weight gain, feed conversion efficiency, and protein efficiency of fishes fed with 0.25% l ‐carnitine diet were observed to be significantly higher. Poor growth was recorded in the fry fed diet with no carnitine, while intermediate growth was observed when fed with 0.50, 0.75, and 1% l ‐carnitine‐incorporated diets. The analysis of body composition of C. mrigala fry indicated that there is no significant difference (P > 0.05) in moisture, protein, and ash contents, but significant difference (P < 0.05) was found in body lipid content of fry, fed with different concentrations of l ‐carnitine. Decrease in viscerosomatic and hepatosomatic indices were observed in fishes fed with l ‐carnitine‐incorporated diets. Results of this study indicated that diet containing 0.25% l ‐carnitine can promote higher growth in C. mrigala fry. 相似文献
20.
鳜皮肤黏液IgM样蛋白的纯化 总被引:3,自引:0,他引:3
对经嗜水气单胞菌全菌疫苗浸泡免疫后的鳜皮肤黏液中的免疫球蛋白采用盐析法、重组蛋白A(HiTrap rProteinA Sepharose)亲和层析法及鼠抗鳜血清IgM单克隆抗体偶联的Sepharose 4B亲和层析法分离纯化,并通过SDS-PAGE及Western-blot技术对纯化蛋白的部分特性进行分析比较。结果表明:50%硫酸铵溶液可以沉淀黏液中大部分蛋白,条带仍较多,约十几条,其中含有72 ku和29 ku的条带,因此仅可作为免疫球蛋白粗提的方法;Sepharose 4B亲和层析法所提取鳜黏液Ig经SDS-PAGE检测,只含有72 ku和29 ku 2个条带(初步认为鳜黏液Ig的重链和轻链),与鳜血清IgM的重、轻链分子量相同;rProteinA亲和层析法所提蛋白除具有上述重链(72 ku)和轻链(29 ku)外,还含有43 ku的蛋白带(可能为鳜黏液Ig另外一种形式的重链)。Western-blot显示,兔抗鳜Ig多克隆抗体可与72 ku及43 ku条带发生发应。两种亲和法所提蛋白纯度较高,但含量较低,条带较淡,仅可作为实验室小量提纯鳜黏液Ig的有效方法。 相似文献