Somaclonal variation in Coffea arabica: effects of genotype and embryogenic cell suspension age on frequency and phenotype of variants

We determined how age of embryogenic cell suspensions affects somaclonal variation in five F1 hybrids of Coffea arabica L. Batches of plants were produced either directly from embryogenic callus, or after 3, 6, 9 and 12 months of embryogenic cell suspension culture. Seven phenotypic variants were characterized. Based on vigor and productivity of the regenerated plants, we classified the variants in order of increasing severity of physiological disorders as: Juvenile leaf color, Giant, Dwarf, Thick leaf (Bullata), Variegata, Angustifolia, and Multi-stem. The Dwarf, Angustifolia and Multi-stem variants were the most frequent among the regenerated plants (1.4, 4.8 and 2.9%, respectively). The frequency (f) of variants increased exponentially with the age (t) of the embryogenic suspension, in accordance with the function f = 0.99e(0.267t). For all genotypes, somaclonal variation was low (1.3%) in plants produced from embryogenic callus or 3-month-old cell suspensions and increased in frequency with increasing suspension age (6, 10 and 25% in plants produced from cell suspensions aged 6, 9 and 12 months, respectively). Large differences in somaclonal variation among genotypes were found only in plants produced from 12-month-old cell suspensions. For two genotypes, the oldest suspensions produced a majority of somaclonal variants (80-90%), whereas somaclonal variation ranged between 8 and 18% in the other genotypes. Cell suspension age and genotype also affected the type of variant produced. The severity of somaclonal variations increased with cell suspension age. For all genotypes combined, the Angustifolia variant was the most common. The other somaclonal variations were specific to certain genotypes or distributed randomly among the genotypes.     

2个陇南大红袍花椒无刺优树无性系对比研究

为给花椒新品种选育提供试验依据,选取‘武选2号’‘武选3号’2个无刺优树嫁接繁育的无性系作为试验对象,与原品种‘大红袍’在物候期、植物学性状、叶片表型性状、果实表型性状及经济性状、皮刺表型性状等方面进行对照试验,结果表明:2个优树无性系其物候期、叶片表型性状、果实表型性状与对照基本一致,且皮刺明显减少,能够提早丰产,选育前景广阔。     

Fruit load and branch ring-barking affect carbon allocation and photosynthesis of leaf and fruit of Coffea arabica in the field

Increasing fruit load (from no berries present to 25, 50 and 100% of the initial fruit load) significantly decreased branch growth on 5-year-old coffee (Coffea arabica L.) trees of the dwarf cultivar 'Costa Rica 95', during their third production cycle. Ring-barking the branches further reduced their growth. Berry dry mass at harvest was significantly reduced by increasing fruit load. Dry matter allocation to berries was four times that allocated to branch growth during the cycle. Branch dieback and berry drop were significantly higher at greater fruit loads. This illustrates the importance of berry sink strength and indicates that there is competition for carbohydrates between berries and shoots and also among berries. Leaf net photosynthesis (P(n)) increased with increasing fruit load. Furthermore, leaves of non-isolated branches bearing full fruit load achieved three times higher P(n) than leaves of isolated (ring-barked) branches without berries, indicating strong relief of leaf P(n) inhibition by carbohydrate demand from berries and other parts of the coffee tree when excess photoassimilates could be exported. Leaf P(n) was significantly higher in the morning than later during the day. This reduction in leaf P(n) is generally attributed to stomatal closure in response to high irradiance, temperature and vapor pressure deficit in the middle of the day; however, it could also be a feedback effect of reserves accumulating during the morning when climatic conditions for leaf P(n) were optimal, because increased leaf mass ratio was observed in leaves of ring-barked branches with low or no fruit loads. Rates of CO(2) emission by berries decreased and calculated photosynthetic rates of berries increased with increasing photosynthetic photon flux (PPF) especially at low PPFs (0 to 100 micromol m(-2) s(-1)). The photosynthetic contribution of berries at the bean-filling stage was estimated to be about 30% of their daily respiration costs and 12% of their total carbon requirements at PPF values commonly experienced in the field (200 to 500 micromol m(-2) s(-1)).     

Ecosystem services from coffee agroforestry in Central America: estimation using the CAF2021 model

The goal of sustainable coffee production requires multiple functions from agroforestry systems. Many are difficult to quantify and data are lacking, hampering the choice of shade tree species and agronomic management. Process-based modelling may help quantify ecosystem services and disservices. We introduce and apply coffee agroforestry model CAF2021 (https://doi.org/10.5281/zenodo.5862195). The model allows for complex systems with up to three shade tree species. It simulates coffee yield, timber and fruit production by shade trees, soil loss in erosion, C-sequestration, N-fixation, -emission and -leaching. To calibrate the model, we used multivariate data from 32 different treatments applied in two long-term coffee agroforestry experiments in Costa Rica and Nicaragua. Without any further calibration, the model was then applied to agroforestry systems on 89 farms in Costa Rica and 79 in Guatemala where yields had been reported previously in farmer interviews. Despite wide variation in environmental and agronomic conditions, the model explained 36% of yield variation in Costa Rica but only 15% in Guatemala. Model analysis quantified trade-offs between yield and other ecosystem services as a function of fertilisation and shading.

     

幼龄枣树叶幕形成规律观察

通过对5年生幼龄枣树叶幕形成全部过程的观察,初步掌握了在咸阳市渭北地区的自然条件下,枣树叶幕形成受外界气温和枣股的发育营养状况影响,及不同年龄枣股上抽生冬吊及着叶特点,从而为幼龄枣树早果、丰产栽培技木提供了参考依据。     

药食两用黄栀子农艺性状的比较及其相关性

为给黄栀子的资源评价及良种选育提供理论参考和指导,对引种栽培的各类型黄栀子的树高、冠幅、叶片性状、果实表型性状以及单株产量等主要农艺性状进行了评价及相关性分析。结果表明:各类型黄栀子农艺性状间差异显著,主要经济性状间存在极显著差异。LH黄栀子多数农艺性状均优于其它类型黄栀子。在果实纵横径、单株果数以及单株产量等方面尤为明显。黄栀子单果鲜质量及单株果数与单株产量呈极显著相关性,相关系数分别可达0.989和0.929;果实纵横径、树高及冠幅与单株产量相关性均达到极显著水平,但萼长及叶片相关性状与产量相关性不显著。     

Somatic embryogenesis of Pinus sylvestris

Embryogenic cultures (EC) of Scots pine (Pinus sylvestris L.) were initiated from immature embryos. Whole ovules were used as expiants. The responsive period for initiation began just after fertilization and remained throughout the development of first stages of early embryos. The main part of the embryogenic cultures were initiated by the time of cleavage polyembryony. Strong correlation was obtained between degree days and the responsive period. During subsequent years the experiments were repeated in Finland and Sweden. In all cases the responsive period for initiating embryogenic cultures was the same, about two weeks after fertilization.

In 1991–1993, a total of 138 clones of elite pine trees were tested for their ability to initiate embryogenic cultures. Of these, 33% were responsive under our experimental conditions. Based on about 300 ovules per clone the number of embryogenic lines induced by the responding clones varied from 0.2% to 9.0% of the expiants.

Several nutrient media were found to be suitable for initiation and proliferation of ECs. About half of the cell lines responded to abscisic acid by producing maturing embryos. The embryos reached full maturity in cultures of only a few cell lines. Some of the embryos that produced roots were planted in soil and transferred to a greenhouse.     

矮化果树在北方的栽培技术探究

矮化果树的栽培技术的发展对于水果产量的提高来说具有重要意义,因此,通过对矮化果树的栽培技术:矮化砧木栽培技术、矮化果树的嫁接技术、生长抑制剂的利用、矮化栽培技术的栽培方式的介绍,以期对矮化果树在北方的栽培技术有所启示.     

榆属树种的叶子结构与抗虫性的关系

榆树是我国重要的造林树种，在全国各地尤其是“三北”地区有大面积栽植。但最近几年来，榆树一直遭受着严重的虫害，特别是食叶害虫榆蓝叶甲，使榆树的生长发育和木材产量受到严重的影响。因此，选育抗虫品种和进行科学管理已成为当务之急。据观察，榆树在遭受虫害后，不同的树种或同一树种的不同无性系间的受害程度差别很大，是什么原因引起受害程度的不同呢？现有的研究己清楚指出，物理因素和化学因素共同构成了植物抵御害虫的防御体系。植物可以利用存在于它们组织的，经长期进化而形成的物理因素（形态结构）来阻止或破坏害虫的为害。本研究采用了电镜扫描和石蜡切片法，结合生物测定和田间调查，分析了榆属树种的叶子结构与其抗虫性之间的关系。结果表明，①榆属树种的气孔以大密度分布在叶子的下表面，气孔的保卫细胞具有角质层和木质化的壁。这种特点使幼虫不易从叶子下表面取食到叶肉，所以它们往往转移到叶子上表面取食，从而造成叶子上表面的结构特点与抗虫性的强弱直接相关；②抗虫性强的树种或无性系的叶子表面有很厚的蜡质层，而易感虫的树种或无性系的叶子上表面蜡质较薄。下表面无此规律；③叶子上表面的表皮毛密度（Ｘ１）和角质层厚度（Ｘ２）与叶面积受害指数（Ｙ）呈高度负相关（     

Fruit load and canopy shading affect leaf characteristics and net gas exchange of 'Spring' navel orange trees

Five-year-old 'Spring' navel (Citrus sinensis (L.) Osbeck) orange trees were completely defruited, 50% defruited or left fully laden to study effects of fruit load on concentrations of nitrogen (N) and carbohydrate, net assimilation of CO2 (Ac) and stomatal conductance (gs) of mature leaves on clear winter days just before fruit harvest. Leaves on defruited trees were larger, had higher starch concentrations and greater leaf dry mass per area (LDMa) than leaves on fruited trees. Both Ac and gs were more than 40% lower in sunlit leaves on defruited trees than in sunlit leaves on trees with fruit. Leaves immediately adjacent to fruit were smaller, had lower leaf nitrogen and carbohydrate concentrations, lower LDMa and lower Ac than leaves on non-fruiting branches of the same trees. Removing half the crop increased individual fruit mass, but reduced fruit color development. Half the trees were shaded with 50% shade cloth for 4 months before harvest to determine the effects of lower leaf temperature (Tl) and leaf-to-air vapor pressure difference on leaf responses. On relatively warm days when sunlit Tl > 25 degrees C, shade increased Ac and gs, but had no effect on the ratio of internal to ambient CO2 (Ci/Ca) concentration in leaves, implying that high mesophyll temperatures in sunlit leaves were more important than gs in limiting Ac. Sunlit leaves were more photoinhibited than shaded leaves on cooler days when Tl < 25 degrees C. Shade decreased total soluble sugar concentrations in leaves, but had no effect on leaf starch concentrations. Shading had no effects on canopy volume, yield or fruit size, but shaded fruit developed better external color than sun-exposed fruit. Overall, the presence of a normal fruit crop resulted in lower foliar carbohydrate concentrations and higher Ac compared with defruited trees, except on warm days when Ac was reduced by high leaf temperatures.     

罗田甜柿优树果实性状的模糊综合评价

为筛选出具有开发潜力的甜柿良种资源,调查了16份罗田甜柿优树果实的主要性状,并运用模糊综合评判法对与甜柿商品性相关的6个主要经济性状指标进行了比较分析。结果表明,16份罗田甜柿果实性状的I_(FC)值在0.056 12~0.528 79之间,其中罗田9号综合评价指数最高,I_(FC)值为0.528 79,与排名第2、第3的17号和14号差距较大。罗田9号单果质量最大、核数量最少,综合评价指数最高,建议作为优良无性系加以开发和利用。     

油茶无性系及自然林选优调查分析

在江西省6个县进行油茶无性系及自然林选优调查分析,结果表明,长林、赣无、赣州油系列无性系优树整体表现出较好的经济性状,主要表现为鲜果含油率、平均冠幅产果量及产油量较高,高产优树较多,自然林优树整体表现低于无性系优树,但存在一部分表现高产的优树,无性系优树间、不同地区自然林优树间、同一地区自然林优树间经济性状差异较大,这为油茶选优工作提供了参考依据和空间。今后油茶选优宜重点从现行推广的无性系中进行优中选优,结合在自然林中进行一定的选择,以达到节约成本、提高效率的目标。     

Physiological and biochemical leaf and tree responses to crop load in apple

Seven-year-old apple (Malus x domestica Borkh.) trees cv. 'Braeburn' on rootstock M.26 were flower-thinned to establish four crop loads, resulting in final mean fruit numbers per tree of 0, 100, 225 and 400. Mean fruit mass decreased by about 35% with each decrease in cropping density. Fruit from light-cropping trees had significantly advanced maturity as indicated by the harvest management criteria of background color and starch/iodine score, and other fruit quality characteristics such as soluble solids. Flesh firmness and dry matter also increased with decreasing crop load. Compared with fruiting trees, mean leaf photosynthetic rates of non-cropping trees were significantly lower (40%) between 75 days after full bloom (dafb) and fruit harvest, with a maximum reduction of almost 60% at 118 dafb. Photosynthetic activity decreased linearly with increasing concentration of leaf starch, but was positively and significantly related to stomatal conductance. Consequently, the accumulation of nonstructural carbohydrates in leaves of light-cropping or non-cropping trees may have led to end-product inhibition of photosynthesis. Increases in xanthophyll cycle carotenoids mediated non-radiative thermal energy dissipation in non-cropping trees, providing increased capacity for photoprotection but reducing photochemical efficiency.     

Morphology and leaf nutrition of introduced Robinia pseudoacacia clones

In order to study and popularize clones from the introduced Robinia pseudoacacia, morphological charac- teristics and leaf nutrition of samples of a three-year-old stand were observed and analyzed during its growing season. Combined with data on rooting ability, height and basal diameter, comparisons were made on morphological characteristics, growth traits and leaf nutrition of 11 introduced R. pseudoacacia clones (two from Hungary and nine from South Korea) and two domestic clones. The results show that there are significant differences in growth and morphological characteristics among the 13 clones, but no significant differences in the contents of crude protein and crude fibers in the leaves (p > 0.05). Height and basal diameter growth of clones B and G were recorded as the fastest, while clone H1 was the slowest. Clone H2 had the largest leaflets, three times as large as other clones, while K4 had the heaviest dry weight per 100 leaflets because of its thick leaves. The 13 clones can be divided into four classes based on the number of leaflets per compound leaf, i.e., 1-3, 13-17, 15-23 and 21-25. There were significant differences in thorn size: H2 , with the largest leaflets had the smallest thorns. While of course all clones produced roots, there were significant differences; clones 2N, K5 and B had many adventitious roots, while clones K3 , K4 and H1 had few. Correlation between content of crude protein and (length × width of leaf) was positive (p < 0.05), while correlations of the content of crude protein, with the number of leaflets per compound leaf, petiole length of compound leaf and thorn length were negative (p < 0.01). Plant height and basal diameter were positively correlated with each other (p < 0.01) and negatively correlated with base width of thorns (p < 0.05) .     

黧蒴优树表型分析及其叶果性状研究

对2003年在全省范围内所选的黧蒴优树进行表型分析，着重研究了其叶、果的性状。结果表明：多个角度证明了5株优势木法选黧蒴优树的方法是合理的，大部分数量指标完全超过5株优势木，体现优中选优的特点。方差分析结果表明：黧蒴优树的叶果性状无论从家系间或种源间均呈极显著差异。动态聚类分析显示黧蒴优树叶果有3种类型，第1类为重果圆叶型，有8个家系；第3类为轻果尖叶型，有90个家系；第2类介于第1、3类之间，有69个家系。     

暖温带不同地区青檀形态特征分析

野生植物资源是人类生存的重要物质基础,随着社会经济的发展和人们对自然资源的过度开发和利用,野生植物资源日趋减少,遗传多样性降低.     

Black leaf streak disease and plantain fruit characteristics as affected by tree density and biomass management in a tropical agroforestry system

Black leaf streak disease (BLSD) affects both bananas and plantains. Anecdotal evidence suggests that when plantain is grown under shade in agroforestry systems, there is less damage from BLSD. In this study, BLSD severity and agronomic fruit characteristics of plantain were compared under high and low timber stand densities (TSD), and also under different crop management systems (mulching, burning, intercropping). By 11 months after planting, in high TSDs, plants had 1.2 more standing leaves, 1.5 more symptom-free leaves and a lower percentage of total leaf area attacked than in low TSDs. At harvest, plants in high TSDs had, on average, 1.4 more standing leaves than those in low TSDs. Percentage leaf area attacked by BLSD was 43.0 % in high TSD treatments, significantly lower than the 57.2 % in low TSD treatments. There was a significant and positive relationship between the number of leaves at flowering and dry fruit bunch mass for plantains grown under low TSD. However, there was no correlation between the number of leaves and fruit mass for the high TSD. The beneficial effects of shade in the high TSD, such as reduced severity of BLSD, were established. However, as there was bunch mass loss associated with reduced leaf production rates under the high TSD, a reduction in BLSD only partially compensated for this yield loss. Thus overall, at the high TSD, there was no yield gain from reduced BLSD.     

Leaf growth characteristics of fast-growing poplar hybrids Populus trichocarpa x P. deltoides

Fast-growing hybrid poplar trees (Populus trichocarpa Torr. & A. Gray x P. deltoides Bartr. ex Marsh.) were compared with slower-growing parental types in both field and laboratory experiments to determine physiological components of leaf growth that could be closely related to biomass production. Stem volume was correlated with individual leaf area (r = 0.81) and leaf growth rate (r = 0.82). Hybrids had a greater total leaf area, not because they produced more leaves, but because they had larger leaves than either parental type. The greater leaf size of the hybrids may be explained by inheritance of larger cell number from P. deltoides and larger cell size from P. trichocarpa. Rates of enlargement of isolated leaf discs in liquid culture were approximately 50% of those observed in intact leaves of field-grown plants.     

Effects of soil water deficit on gas exchange characteristics and water relations of orchard lychee (Litchi chinensis Sonn.) trees

Eight-year-old lychee (Litchi chinensis Sonn.) trees, cv. 'Bengal,' growing in krasnozem soil were subjected to soil water deficit from one month before flowering until harvest by covering the ground with polyethylene sheeting and withholding irrigation. The ratio of daytime stomatal conductance of unirrigated to irrigated trees decreased 20% during the three months of increasing water deficit. Predawn leaf water potentials of irrigated trees averaged about -0.3 MPa throughout the period, whereas they declined progressively to -0.9 MPa in unirrigated trees. Minimum daytime leaf water potential in the unirrigated trees decreased from -1.0 to -1.1 MPa at the beginning of the drought period to -2.2 to -2.4 MPa after three months, and calculated whole-plant conductance did not change with decreasing availability of water. The calculated soil-root water potential declined to less than -1.0 MPa in unirrigated trees. Capacitance effects on the relationship between leaf water potential and transpiration were significant only at low transpiration rates. Although unirrigated trees reduced soil water content at 0-30 cm depths to an equivalent water potential of -1.0 MPa, fruit shedding was significantly less than in irrigated trees. Water deficit had no effect on the fresh weight of pericarp, but caused increased seed size and decreased fresh weight of flesh, resulting in fruit from unirrigated trees being 16% lower in total fresh weight per fruit than fruit from irrigated trees.