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1.
Effects of irrigation deprivation during the harvest period on yield determinants in mature almond (Prunus dulcis (Mill.) D.A. Webb cv. Nonpareil) trees were investigated during a 3-year field experiment. Return bloom and fruit set were measured on 2185 individually tagged spurs. Water stress resulting from irrigation deprivation during the harvest period, which purportedly coincides with the time of flower initiation, had no effect on the percentage of spurs that flowered or set fruit during subsequent years. Although water stress had no apparent effect on spur mortality, 66% of the tagged spurs died within 3 years. In addition, many spurs were vegetative by the third year, indicating the importance of spur renewal for sustained fruit production. Reductions in nut yield were evident after two successive years of irrigation deprivation during the harvest period. Regression analysis indicated a loss in yield of 7.7 kg tree(-1) in response to each 1 MPa decrease in stem water potential below -1.2 MPa during the previous seasons. The number of fruiting positions per tree (estimated indirectly for whole trees based on weight of current-year shoots > 5 cm in length) was negatively associated with water stress. Yield reduction in response to water stress during harvest appears to be a compound, multiyear effect, associated with reduced annual growth and renewal of fruiting positions. 相似文献
2.
Effect of irrigation deprivation during the harvest period on the nonstructural carbohydrate (NC) content of dormant, mature, field-grown almond (Prunus dulcis (Mill.) D.A. Webb cv. Nonpareil) trees was studied. Roots, trunk, branches, spurs and stems of 12 trees were subsampled in February 1997, across a gradient of irrigation treatments (FI = fully irrigated, MS = moderately stressed and SS = severely stressed) to relate NC concentration to the degree of water stress experienced by individual trees during the previous (1996) harvest period. To assess the effect of water stress on whole-tree NC content, three dormant FI trees and three dormant SS trees were excavated on December 10, 1997, and dry weights and NC and N concentrations of the tree components were determined. Whole-tree biomass did not differ significantly between FI and SS trees, although SS trees tended to have less total dry weight. Although roots constituted just 13% of tree biomass, they stored 36 and 44% of tree NC and N contents, respectively. There were negative relationships between the seasonal minimum values of both midday (Psi(ms)) and predawn (Psi(pd)) stem water potentials during the harvest period and root NC content of dormant trees. Severe water stress during the harvest period resulted in a 26% reduction in NC content and a 50% reduction in biomass of current-year stems (> 5 cm in length) per tree. The reduction in NC content is consistent with the previously reported late season reductions in leaf function and persistence. The SS trees exhibited a reduction in NC content but not in N content per tree, indicating that late season accumulation of NC and N were uncoupled in trees subjected to severe harvest-period water stress. 相似文献
3.
Effects of regulated deficit irrigation during the pre-harvest period on gas exchange, leaf development and crop yield of mature almond trees 总被引:1,自引:0,他引:1
We investigated the effects of regulated deficit irrigation (RDI) during the pre-harvest period (kernel-filling stage) on water relations, leaf development and crop yield in mature almond (Prunus dulcis (Mill.) D.A. Webb cv. Cartagenera) trees during a 2-year field experiment. Trees were either irrigated at full-crop evapotranspiration (ETc=100%) (well-irrigated control treatment) or subjected to an RDI treatment that consisted of full irrigation for the full season, except from early June to early August (kernel-filling stage), when 20% ETc was applied. The severity of water stress was characterized by measurements of soil water content, predawn leaf water potential (Psipd) and relative water content (RWC). Stomatal conductance (gs), net CO2 assimilation rate (A), transpiration rate (E), leaf abscission, leaf expansion rate and crop yield were also measured. In both years, Psipd and RWC of well-irrigated trees were maintained above -1.0 MPa and 92%, respectively, whereas the corresponding values for trees in the RDI treatment were -2.37 MPa and 82%. Long-term water stress led to a progressive decline in gs, A and E, with significant reductions after 21 days in the RDI treatment. At the time of maximum stress (48 days after commencement of RDI), A, gs and E were 64, 67 and 56% lower than control values, respectively. High correlations between A, E and gs were observed. Plant water status recovered within 15 days after the resumption of irrigation and was associated with recovery of soil water content. A relatively rapid and complete recovery of A and gs was also observed, although the recovery was slower than for Psipd and RWC. Severe water stress during the kernel-filling stage resulted in premature defoliation (caused by increased leaf abscission) and a reduction in leaf growth rate, which decreased tree leaf area. Although kernel yield was correlated with leaf water potential, RDI caused a nonsignificant 7% reduction in kernel yield and had no effect on kernel size. The RDI treatment also improved water-use efficiency because about 30% less irrigation water was applied in the RDI treatment than in the control treatment. We conclude that high-cropping almonds can be successfully grown in semiarid regions in an RDI regime provided that Psipd is maintained above a threshold value of -2 MPa. 相似文献
4.
Egea G González-Real MM Baille A Nortes PA Conesa MR Ruiz-Salleres I 《Tree physiology》2012,32(4):450-463
Photosynthetic acclimation to highly variable local irradiance within the tree crown plays a primary role in determining tree carbon uptake. This study explores the plasticity of leaf structural and physiological traits in response to the interactive effects of ontogeny, water stress and irradiance in adult almond trees that have been subjected to three water regimes (full irrigation, deficit irrigation and rain-fed) for a 3-year period (2006-08) in a semiarid climate. Leaf structural (dry mass per unit area, N and chlorophyll content) and photosynthetic (maximum net CO(2) assimilation, A(max), maximum stomatal conductance, g(s,max), and mesophyll conductance, g(m)) traits and stem-to-leaf hydraulic conductance (K(s-l)) were determined throughout the 2008 growing season in leaves of outer south-facing (S-leaves) and inner northwest-facing (NW-leaves) shoots. Leaf plasticity was quantified by means of an exposure adjustment coefficient (ε=1-X(NW)/X(S)) for each trait (X) of S- and NW-leaves. Photosynthetic traits and K(s-l) exhibited higher irradiance-elicited plasticity (higher ε) than structural traits in all treatments, with the highest and lowest plasticity being observed in the fully irrigated and rain-fed trees, respectively. Our results suggest that water stress modulates the irradiance-elicited plasticity of almond leaves through changes in crown architecture. Such changes lead to a more even distribution of within-crown irradiance, and hence of the photosynthetic capacity, as water stress intensifies. Ontogeny drove seasonal changes only in the ε of area- and mass-based N content and mass-based chlorophyll content, while no leaf age-dependent effect was observed on ε as regards the physiological traits. Our results also indicate that the irradiance-elicited plasticity of A(max) is mainly driven by changes in leaf dry mass per unit area, in g(m) and, most likely, in the partitioning of the leaf N content. 相似文献
5.
Altitudinal differences in the leaf fitness of juvenile and mature alpine spruce trees (Picea crassifolia) 总被引:4,自引:0,他引:4
In many plant species, leaf morphology varies with altitude, an effect that has been attributed to temperature. It remains uncertain whether such a trend applies equally to juvenile and mature trees across altitudinal gradients in semi-arid mountain regions. We examined altitude-related differences in a variety of needle characteristics of juvenile (2-m tall) and mature (5-m tall) alpine spruce (Picea crassifolia Kom.) trees growing at altitudes between 2501 and 3450 m in the Qilian Mountains of northwest China. We found that stable carbon isotope composition (delta(13)C), area- and mass-based leaf nitrogen concentration (N(a), N(m)), number of stomata per gram of nitrogen (St/N), number of stomata per unit leaf mass (St/LM), projected leaf area per 100 needles (LA) and leaf mass per unit area (LMA) varied nonlinearly with altitude for both juvenile and mature trees, with a relationship reversal point at about 3100 m. Stomatal density (SD) of juvenile trees remained unchanged with altitude, whereas SD and stomatal number per unit length (SNL) of mature spruce initially increased with altitude, but subsequently decreased. Although several measured indices were generally found to be higher in mature trees than in juvenile trees, N(m), leaf carbon concentration (C(m)), leaf water concentration (LWC), St/N, LA and St/LM showed inconsistent differences between trees of different ages along the altitudinal gradient. In both juvenile and mature trees, delta(13)C correlated significantly with LMA, N(m), N(a), SNL, St/LM and St/N. Stomatal density, LWC and LA were only significantly correlated with delta(13)C in mature trees. These findings suggest that there are distinct ecophysiological differences between the needles of juvenile and mature trees that determine their response to changes in altitude in semi-arid mountainous regions. Variations in the fitness of forests of different ages may have important implications for modeling forest responses to changes in environmental conditions, such as predicted future temperature increases in high altitude areas associated with climate change. 相似文献
6.
In mature almond (Prunus dulcis) orchards, the majority of crop is borne on spurs (short, proleptic shoots) that can live for several years and can produce from one to five fruits. Previous research has led to the hypothesis that spur longevity is related to spur light exposure, cropping and age. However, limited quantitative data are available to substantiate these hypotheses. The objective of this study was to determine spur characteristics that were most highly correlated with spur productivity and longevity in mature, bearing almond trees. Previous year spur leaf area was strongly related to spur viability and flowering; the greater the leaf area in the previous year, the higher the probability of spur survival into the next year and the higher the probability for the spur to bear one or more flowers. Previous year bearing also appeared to influence viability and return bloom, especially in spurs with low leaf area. These results suggest that spur source-sink balance is basic to the life cycle of almond spurs. Furthermore, the results are consistent with the hypothesis that spurs are semi-autonomous organs with respect to carbohydrate balance for much of the growing season. Finally, this information provides general thresholds for maintaining spur viability and productivity that will be useful for developing and evaluating tree training systems and orchard management practices. 相似文献
7.
We estimated the amount of nitrogen (N) remobilized from 1-year-old leaves at various positions in the crowns of mature Quercus glauca Thunb. ex Murray trees and related this to the production of new shoots. Leaf N concentration on an area basis (Na) and total N (Nt= Na x lamina area of all leaves on a shoot) were related to photosynthetic photon flux (PPF) on the leaves of current-year and 1-year-old shoots. When new shoots (S02 shoots; flushed in 2002) flushed, only a portion of the leaves on the previous year's shoots (S01 shoots; flushed in 2001) were shed. After the S02 shoots flushed, S01 shoots were defined as 1-year-old shoots (S01* shoots). Both Na and Nt were positively correlated with PPF for S01 shoots, but not for S01* shoots. The fraction of remobilized N (% of the maximum Na in S01 leaves) from remaining leaves was 5-35%, with the fraction size being positively correlated with the number of S02 shoots on an S01* shoot (new shoot number). However, the mean fraction of remobilized N from fallen leaves was 45% and was unrelated to new shoot number. The total amount of N remobilized from both fallen and remaining leaves was 1-20 mg per S01* shoot. Total remobilized N was positively correlated with new shoot number. There was a statistically significant positive relationship between the light-saturated net photosynthetic rate on a leaf area basis (Amax) and Na for both S01* and S02 leaves. However, when we compared leaves with similar Na, Amax of S01* leaves was only half that of S02 leaves, indicating that 1-year-old leaves had lower instantaneous N-use efficiency (Amax per unit Na) than current-year leaves. Ratios of chlorophyll a:b and Rubisco:chlorophyll were lower in S01* leaves than in S02 leaves, indicating that 1-year-old leaves were acclimatized to lower light environments. Thus, in Q. glauca, the N allocation theory (i.e., that N is distributed according to local PPF) applied only to the current-year shoots. Although the amount of foliar N in 1-year-old shoots was not strongly affected by the PPF on 1-year-old leaves, it was affected by interactions with current-year shoots. 相似文献
8.
Seasonal variations in leaf nitrogen, phosphorus and potassium concentrations were studied in a mature carob (Ceratonia siliqua L. cv "Mulata") orchard subjected to a 4-year irrigation and fertilization experiment. Three irrigation regimes (0, 50 and 100%), based on the evaporation values obtained from a class A pan, were tested in combination with two nitrogen (N) supply regimes in which 21 kg ha(-1) year(-1) (low-N) and 63 kg ha(-1) year(-1) (high-N) were supplied as ammonium nitrate. Leaf nitrogen concentration increased throughout the experiment, independently of treatments. There were no significant differences in leaf N concentration between trees in the high-N and low-N treatments. Irrigation regimes had no effect on leaf mineral concentration but influenced the amount of leaves shed and slightly modified the pattern of leaf shedding that occurred during the summer drought period. Nutritional balances between N and P and N and K were both closely and significantly correlated. Potassium was translocated from leaves to fruits during spring, independently of treatments. Severe water stress periods occurring during spring or autumn induced shedding of leaves leading to nutrient mobilization. Nutrient retranslocation during these drought periods may represent an adaptive mechanism. Nitrogen retranslocation was higher for trees in the high-N treatments than for trees in the low-N treatments, whereas phosphorus retranslocation was independent of the irrigation and fertilization treatments. 相似文献
9.
We examined the vertical profiles of leaf characteristics within the crowns of two late-successional (Fagus crenata Blume and Fagus japonica Maxim.) and one early-successional tree species (Betula grossa Sieb. et Zucc.) in a Japanese forest. We also assessed the contributions of the leaves in each crown layer to whole-crown instantaneous carbon gain at midday. Carbon gain was estimated from the relationship between electron transport and photosynthetic rates. We hypothesized that more irradiance can penetrate into the middle of the crown if the upper crown layers have steep leaf inclination angles. We found that such a crown has a high whole-crown carbon gain, even if leaf traits do not change greatly with decreasing crown height. Leaf area indices (LAIs) of the two Fagus trees (5.26-5.52) were higher than the LAI of the B. grossa tree (4.50) and the leaves of the F. crenata tree were more concentrated in the top crown layers than were leaves of the other trees. Whole-crown carbon gain per unit ground area (micromol m(-2) ground s(-1)) at midday on fine days in summer was 16.3 for F. crenata, 11.0 for F. japonica, and 20.4 for B. grossa. In all study trees, leaf dry mass (LMA) and leaf nitrogen content (N) per unit area decreased with decreasing height in the crown, but leaf N per unit mass increased. Variations (plasticity) between the uppermost and lowermost crown layers in LMA, leaf N, the ratio of chlorophyll to N and the ratio of chlorophyll a to b were smaller for F. japonica and B. grossa than for F. crenata. The light extinction coefficients in the crowns were lower for the F. japonica and B. grossa trees than for the F. crenata tree. The leaf carbon isotope ratio (delta(13)C) was higher for F. japonica and B. grossa than for F. crenata, especially in the mid-crown. These results suggest that, in crowns with low leaf plasticity but steep leaf inclination angles, such as those of F. japonica and B. grossa trees, irradiance can penetrate into the middle of the crowns, thereby enhancing whole-crown carbon gain. 相似文献
10.
In Alberta, Canada, pairs of grafted lodgepole pine trees were selected to study the longevity and location of live roots of snags that were grafted to living trees, to determine the impact of these live residual roots on the diameter growth of the living tree. In a second study, dense groups of grafted trees were manually thinned and one leave tree was left to grow for two growing seasons. For both studies, roots were excavated. Results indicate that more live roots were maintained on snags connected to living trees with a large graft and that roots located within 90° of the root grafted to the live tree persisted longer. Also, tree ring index in the living trees significantly increased following manual thinning, but was unaffected when the grafted partner died naturally. Grafts with large phloem connections maintained a higher number of live roots on snags, than grafts with small connections. 相似文献
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12.
Branches of nine-year-old loblolly pine trees grown in a 2 x 2 factorial combination of fertilization and irrigation were exposed for 11 months to ambient, ambient + 175, or ambient + 350 micro mol mol(-1) CO(2). Rates of light-saturated net photosynthesis (A(max)), maximum stomatal conductance to water vapor (g(max)), and foliar nitrogen concentration (% dry mass) were assessed monthly from April 1993 until September 1993 on 1992 foliage (one-year-old) and from July 1993 to March 1994 on 1993 foliage (current-year). Rates of A(max) of foliage in the ambient + 175 CO(2) treatment and ambient + 350 were 32-47 and 83-91% greater, respectively, than that of foliage in the ambient CO(2) treatment. There was a statistically significant interaction between CO(2) treatment and fertilization or irrigation treatment on A(max) on only one measurement date for each age class of foliage. Light-saturated stomatal conductance to water vapor (g(max)) was significantly affected by CO(2) treatment on only four measurement dates. Light-saturated g(max) in winter was only 42% of summer g(max) even though soil water during winter was near field capacity and evaporative demand was low. Fertilization increased foliar N concentration by 30% over the study period when averaged across CO(2) treatments. During the study period, the ambient + 350 CO(2) treatment decreased average foliar N concentration of one-year-old foliage in the control, irrigated, fertilized and irrigated + fertilized plots by 5, 6.4, 9.6 and 11%, respectively, compared with one-year-old foliage in the corresponding ambient CO(2) treatments. The percent increase in A(max) due to CO(2) enrichment was similar in all irrigation and fertilization treatments and the effect persisted throughout the 11-month study period for both one-year-old and current-year foliage. 相似文献
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14.
To better understand the effects of sugar accumulation on red color development of foliage during autumn, we compared carbohydrate concentration, anthocyanin expression and xylem pressure potential of foliage on girdled versus non-girled (control) branches of 12 mature, open-grown sugar maple (Acer saccharum Marsh.) trees. Half of the study trees were known to exhibit mostly yellow foliar coloration and half historically displayed red coloration. Leaves from both girdled and control branches were harvested at peak color expression (i.e., little or no chlorophyll present). Disruption of phloem export by girdling increased foliar sucrose, glucose and fructose concentrations regardless of historical tree color patterns. Branch girdling also increased foliar anthocyanin expression from 50.4 to 66.7% in historically red trees and from 11.7 to 54.2% in historically yellow trees, the latter representing about a fivefold increase compared with control branches. Correlation analyses indicated a strong and consistent relationship between foliar red coloration and sugar concentrations, particularly glucose and fructose, in both girdled and control branches. Measures of xylem pressure potentials confirmed that girdling was a phloem-specific treatment and had no effect on water transport to distal leaves. Results indicate that stem girdling increased foliar sugar concentrations and enhanced anthocyanin expression during autumn in sugar maple foliage. Native environmental stresses (e.g., low autumn temperatures) that reduce phloem transport may promote similar physiological outcomes. 相似文献
15.
Guerrero J Moriana A Pérez-López D Couceiro JF Olmedilla N Gijón MC 《Tree physiology》2006,26(1):87-92
Recovery of water status in water-stressed pistachio trees (Pistacia vera L. cv. Kerman) was investigated by subjecting trees to regulated deficit irrigation (RDI) (60% of crop evapotranspiration rate, ET(c)) during stages I and II of fruit development (FD) followed by full irrigation during FD stage III (kernel-filling). Trees irrigated at 100% ET(c) throughout FD stages I, II and III served as controls. Water-stress severity was characterized by changes in soil water content and midday stem water potential (Psi(md)). Midday leaf conductance (g(1)) and trunk diameter variation (TDV) were also measured. In RDI trees, the lowest Psi(md) value, -1.8 MPa, occurred at the end of the RDI period. The corresponding value for the control trees was around -1.1 MPa. Although the RDI treatment affected gas exchange later than Psi(md), the greatest reductions in gas exchange (60% of control values) also appeared at the end of the RDI period. There were significant differences in TDV between control and RDI trees at the end of the RDI period. Although plant water status recovered within 20 days of resuming irrigation, the TDV values indicated a longer period might be necessary for complete recovery. Recovery of g(1) was faster than that of Psi(md), although differences in TDV between control and RDI trees indicated that gas exchange recovered later than Psi(md). The slow recovery of pistachio trees during FD stage III from water stress imposed during FD stages I and II suggests that irrigation should exceed 100% ET(c) during FD stage III or that more extensive irrigation should commence before the end of FD stage II. 相似文献
16.
In a field study, we compared anatomy and diurnal gas exchange and chlorophyll fluorescence in sunlit mature leaves of Macaranga gigantea (Reichb. f. and Zoll.) Muell. seedlings, saplings, an adult tree and suckers originating from stumps. We tested the hypothesis that the pattern of resource use shifts across various life stages with ontogenetic changes in leaf anatomy and physiology. Among leaves of different developmental stages, seedling leaves were the smallest and thinnest, whereas adult tree leaves were the largest and thickest, and the air space within the lamina was largest in seedling leaves and smallest in adult tree leaves. Photosynthetic nitrogen-use efficiency (PNUE) was higher in seedling and sapling leaves than in adult tree leaves. Mean PNUE in seedling leaves was 1.6 times that in adult tree leaves. Nevertheless, among the developmental stages, net photosynthetic rate (Pn) per unit leaf area was lowest in seedling leaves because they have the lowest nitrogen (N) content per unit leaf area. In situ water vapor stomatal conductance (g(s) at a given leaf-to-air vapor pressure deficit was highest in sapling leaves, suggesting that they have a high hydraulic efficiency per unit leaf area. Among developmental stages, intrinsic water-use efficiency (Pn/g(s)) and photochemical capacity of photosystem II were lowest in seedling leaves. Sapling leaves had the highest N concentration and Pn per unit dry mass and the highest g(s), indicating that the gradual transition from the seedling stage to the sapling stage is accompanied by an accumulation of N in plant bodies and the development of hydraulic systems to counteract unfavorable environmental stresses. The properties of adult tree leaves (low PNUE, high carbon:N ratio, small and dense cells and thick lamina) indicate that, during the transition from the sapling stage to the adult tree stage, the priority of resource use in leaves gradually shifts from enhancement of photosynthetic performance to defense against herbivory and mechanical damage. Leaf morphology and physiology were coordinated with the differences in resource use at each life stage. 相似文献
17.
Single cells were mechanically isolated from leaf-derived callus of mature Juniperus oxycedrus L. These cells divided and gave rise to callus when plated on medium containing growth regulators. Best plating efficiency was obtained on a modified Schenk and Hildebrandt medium supplemented with 0.6 micro M 2,4-dichlorophenoxyacetic acid and 100 mg l(-1) casein hydrolyzate. Although single-cell-derived callus showed poor morphogenic potential, both adventitious shoots and embryogenic tissues differentiated from the callus. We also achieved induction of somatic embryogenesis in leaf explants of mature J. oxycedrus trees cultured in the presence of 6.0 or 10.0 micro M 2,4-dichlorophenoxyacetic acid or picloram. Frequency of embryogenic callus ranged from 6 to 18%; however, under the culture conditions tested, isolated embryos failed to develop into plants. 相似文献
18.
Changes in gas exchange with leaf age and fruit growth were determined in lychee trees (Litchi chinensis Sonn.) growing in subtropical Queensland (27 degrees S). Leaves expanded in a sigmoid pattern over 50 days during spring, with net CO2 assimilation (A) increasing from -4.1 +/- 0.9 to 8.3 +/- 0.5 micromol m-2 s-1 as the leaves changed from soft and red, to soft and light green, to hard and dark green. Over the same period, dark respiration (Rd) decreased from 5.0 +/- 0.8 to 2.0 +/- 0.1 micromol CO2 m-2 s-1. Net CO2 assimilation was above zero about 30 days after leaf emergence or when the leaves were half fully expanded. Chlorophyll concentrations increased from 0.7 +/- 0.2 mg g-1 in young red leaves to 10.3 +/- 0.7 mg g-1 in dark green leaves, along with stomatal conductance (gs, from 0.16 +/- 0.09 to 0.47 +/- 0.17 mol H2O m-2 s-1). Fruit growth was sigmoidal, with maximum values of fresh mass (29 g), dry mass (6 g) and fruit surface area (39 cm2) occurring 97 to 115 days after fruit set. Fruit CO2 exchange in the light (Rl) and dark (Rd) decreased from fruit set to fruit maturity, whether expressed on a surface area (10 to 3 micromol CO2 m-2 s-1 and 20 to 3 micromol CO2 m-2 s-1, respectively) or on a dry mass basis (24 to 2 nmol CO2 g-1 s-1 and 33 to 2 nmol CO2 g-1 s-1, respectively). Photosynthesis never exceeded respiration, however, the difference between Rl and Rd was greatest in young green fruit (4 to 8 micromol CO2 m-2 s-1). About 90% of the carbon required for fruit growth was accounted for in the dry matter of the fruit, with the remainder required for respiration. Fruit photosynthesis contributed about 3% of the total carbon requirement of the fruit over the season. Fruit growth was mainly dependent on CO2 assimilation in recently expanded dark green leaves. 相似文献
19.