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1.
Frego (fg) bract is an important agronomic trait in tetraploid cotton, which has been widely introduced into several cotton varities
or lines in the past several years. In order to help us further understand the underlying molecular mechanism of frego bract
development, a map-base cloning strategy was used to localize the fg locus. An F2 population which comprised of 290 fg individuals derived from a cross of the multiple-marker line T582 (G. hirsutum, carrying the fg gene) with Hai7124 (G. barbadense) was constructed. Genetic linkage analysis was carried out to map of the fg locus with SSR and EST-SSR markers in tetraploid cotton. Genetic linkage analysis showed that the fg locus was flanked by the marker NAU3016 and NAU3172 on the long arm of chromosome 3, with the genetic distance of 0.3 cM
and 4.7 cM, respectively. The information of fg locus provided the basic information for the final isolation of this important gene in tetraploid cotton, these marker information
could be used in marker-assisted selection in cotton. 相似文献
2.
Cytogenetic characterization by karyotyping and determination of DNA content by flow cytometry of seven cultivated varieties
of Chenopodium was performed. Chenopodium quinoa cultivar Barandales and C. berlandieri subsp. nuttalliae cultigens Huauzontle, Quelite and Chia roja showed 2n = 4x = 36, x = 9. Statistically insignificant genome size differences for studied varieties ranged from 2.96 pg/2C (1 Cx = 724 Mbp) in C. quinoa to 3.04 pg/2C (1 Cx = 743 Mbp) in Huauzontle. Karyotype analyses revealed the presence of nine groups of four metacentric chromosomes, including
two pairs of chromosomes with satellites. The first pair of satellites was located on the largest pair of chromosomes and
the second on a different pair of chromosomes in all accessions analyzed. Variation among varieties was evident in chromosome
size, genome length (GL) and the position of satellites. Chia roja exhibited greatest GL (58.82 μm) and biggest chromosomes
(2.04 μm). Huauzontle showed the smallest GL (45.02 μm) and shortest chromosomes (1.60 μm). Comparison of GL in studied taxa
was statistically significant and allowed to define three groups according to the use given to these plants. These data indicate
that they are small, very stable genomes in terms of DNA content, and they support the allotetraploid origin(s) of C. berlandieri subsp. nuttalliae and C. quinoa. 相似文献
3.
Effects of Brassica napus (N) and B. juncea (J) cytoplasm on seed characteristics of B. carinata (C) were examined. Alloplasmic lines of B. carinata were produced from N × C and J × C hybrids by recurrent backcrossing to the BC8 generation. Fourteen sets of reciprocal crosses were used. Compared with their euplasmic sibs, alloplasmic B. carinata line seeds with B. napus cytoplasm showed reduced dormancy, higher seed weight, lower germination rate at high temperatures, higher germination rate
at low temperatures, and had lower erucic acid and higher linoleic acid contents. Alloplasmic B. carinata line seeds with B. juncea cytoplasm had higher seed weight but lower germination rate than their corresponding euplasmic sibs. These results showed
a cytoplasmic effect on seed development, and an influence on seed weight, dormancy, and fatty acid composition. B. carinata was more deleteriously affected by cytoplasm from B. napus than by cytoplasm of B. juncea. 相似文献
4.
Use of cross incompatibility in corn (Zea mays L.) by the Ga1-s allele may reduce cross-fertilization in specialty and conventional organic corn with pollen from genetically-modified (GM)
corn. For effective use, information about environment, and genotype × environment effects on cross-fertilization by ga1 as well as heritability of cross incompatibility in maize is necessary. Our objective was to obtain this information. Four
population pairs differing in their genotype at ga1 were evaluated for cross incompatibility with ga1 pollen in different environments. Populations were derived by crossing the recurrent parents B116, PHG35, ARZM16035:S19,
and (CHZM05015:Mo17)Mo17 to Ga1-s donor parent Mo508W/Mo506W. Two replicates of each treatment were grown in the center of 952 m2 fields planted with purple corn as an adventitious source of ga1/ga1 pollen. Open pollination was allowed and the amount of cross-fertilization estimated by averaging the percentage of purple
seeds. Environment and genotype × environment effects were not significant. Contrasts to evaluate differences in cross-fertilization
between Ga1-s and ga1 populations revealed that mean percentages of cross-fertilization in Ga1-s populations of B116, ARZM16035:S19, and (CHZM05015:Mo17)Mo17 were significantly lower than in ga1 populations. The estimated broad-sense heritability on an entry-mean basis for cross incompatibility was 0.81. Results suggest
differences in genotype at ga1 played a major role in cross-fertilization of populations differing in their genotype at the ga1 locus. Incompatibility may be selected effectively over different environments and the Ga1-s system may be of value to reduce cross-fertilization with GM corn pollen. 相似文献
5.
Joseph M. Anderson Dennis L. Bucholtz Nagesh Sardesai Judith B. Santini Gábor Gyulai Christie E. Williams Stephen B. Goodwin 《Euphytica》2010,172(2):251-262
Lophopyrum species carry many desirable agronomic traits, including disease resistance, which can be transferred to wheat by interspecific
hybridization. To identify potentially new genes for disease and insect resistance carried by individual Lophopyrum chromosomes, 19 of 21 possible wheat cultivar Chinese Spring × Lophopyrum elongatum disomic substitution lines were tested for resistance to barley yellow dwarf virus (BYDV), cereal yellow dwarf virus (CYDV),
the Hessian fly Mayetiola destructor, and the fungal pathogens Blumeria graminis and Mycosphaerella graminicola (asexual stage: Septoria tritici). Low resistance to BYDV occurred in some of the disomic substitution lines, but viral titers were significantly higher than
those of two Lophopyrum species tested. This suggested that genes on more than one Lophopyrum chromosome are required for complete resistance to this virus. A potentially new gene for resistance to CYDV was detected
on wheatgrass chromosome 3E. All of the substitution lines were susceptible to Mayetiola destructor and one strain of B. graminis. Disomic substitution lines containing wheatgrass chromosomes 1E and 6E were significantly more resistant to M. graminicola compared to Chinese Spring. Although neither chromosome by itself conferred resistance as high as that in the wheatgrass parent, they do appear to contain
potentially new genes for resistance against this pathogen that could be useful for future plant-improvement programs. 相似文献
6.
Lian-Quan Zhang Deng-Cai Liu You-Liang Zheng Ze-Hong Yan Shou-Fen Dai Yun-Fang Li Qi Jiang Ya-Qing Ye Yang Yen 《Euphytica》2010,172(2):285-294
Spontaneous chromosome doubling via union of unreduced (2n) gametes has been thought to be the way that common wheat (Triticum aestivum L.) was originated from the hybridization of T. turgidum L. with Ae. tauschii Cosson. Previous works have observed unreduced gametes in F1 hybrids of Ae. tauschii with six of the eight T. turgidum subspecies. It is not clear, however, whether the formation of these unreduced gametes is a norm in the F1 hybrids. In the present study, we tried to answer this question by assessing the occurrence frequency of unreduced gametes
in 115 T. turgidum–Ae. tauschii hybrid combinations, involving 76 genotypes of seven T. turgdium subspecies and 24 Ae. tauschii accessions. Our data show that these hybrid combinations differed significantly (P ≤ 0.01, F = 11.40) in selfed seedset, an indicator for production of unreduced gametes. This study clearly showed that meiotic restitution
genes are widely distributed within T. turgidum. However, significant differences were found between as well as within T. turgidum subspecies and in the interaction of the T. turgidum genotypes with those of Ae. taushii. The possible application of the meiotic restitution genes from T. turgidum in production of double haploids is also discussed. 相似文献
7.
In traditional quantitative genetics, additive effects of genes acting in a population of biparental homozygous lines are
estimated on the basis of the phenotypic observations only, usually by taking a difference between mean values for extreme
lines. Current molecular methods allow to estimate the additive effects by additionally taking into account the marker data.
In this paper we compare these two methods of estimation of additive gene action effects analytically, by simulations and
by analysis of real data sets for doubled haploid lines and recombinant inbred lines. The analytic comparison shows under
which conditions an agreement of the two methods can be achieved. In most of the considered experimental data and in simulations
we observe that the additive effect calculated on the basis of the marker observations is smaller than the total additive
effect obtained from phenotypic observations only. This result is discussed, and a weighted regression approach is proposed
as a method which can close the gap between the purely phenotypic and genotypic approaches. 相似文献
8.
Akhilesh Sharma Rajeev Rathour P. Plaha Viveka Katoch G. S. Khalsa Vandana Patial Yudhvir Singh N. K. Pathania 《Euphytica》2010,173(3):345-356
Wilt caused by Fusarium oxysporum f. sp. pisi is a serious production constraint for peas worldwide. An attempt was made to isolate wilt-resistant mutants in two susceptible
pea genotypes, Arkel and Azad P-1, employing induced mutagenesis and in vitro selection techniques. Two thousand seeds of
each genotype were mutagenized either with ethyl methane sulfonate (EMS, 0.2% and 0.3%) or gamma rays (5-22.5 kR) in 60Co gamma cell for three consecutive years. Screening of different mutagenized populations under wilt-sick plots resulted in
the isolation of 25 mutants exhibiting complete or enhanced wilt resistance compared to parental genotypes. Five of these
wilt-resistant mutants also outperformed the susceptible background genotypes in terms of yield and other horticultural traits.
Efforts were also made to isolate wilt-resistant regenerants from callus cultures exhibiting insensitivity to culture filtrate
(CF) of F. oxysporum f. sp. pisi. A total of 250 regenerants (R
0) were obtained from CF-insensitive calli selected from medium supplemented with 20% culture filtrate. When evaluated in artificially
inoculated sick plots, only five R
2 lines obtained from the regenerants exhibited enhanced wilt resistance compared to parental cultivars. However, the selected
lines did not exhibit resistance levels equivalent to those shown by wilt-resistant lines isolated through in vivo mutagenesis.
To conclude, induced mutagenesis through irradiation and EMS treatments exhibited superiority over in vitro selection for
inducing wilt resistance in peas. 相似文献
9.
Jing wen Jin-xing Tu Zai-yun Li Ting-dong Fu Chao-zhi Ma Jin-xiong Shen 《Euphytica》2008,162(1):81-89
The primary aim of this study was to optimize in vitro culture protocols to establish an efficient reproducible culture system
for different Brassica interspecific crosses, and to synthesize yellow-seeded Brassica napus (AACC) for breeding and genetical studies. Reciprocal crosses were carried out between three B. rapa L. ssp. oleifera varieties (AA) and five accessions of B. oleracea var. acephala (CC). All the parental lines were yellow-seeded except one accession of B. oleracea. Hybrids were obtained through either ovary culture from crosses B. rapa × B. oleracea, or embryo culture from crosses B. oleracea × B. rapa. A higher rate of hybrid production was recorded when ovaries were cultured at 4–7 days after pollination (DAP). Of different
culture media, medium E (MS with half strength macronutrients) showed good response for ovaries from all the crosses, the
highest rate of hybrid production reaching 45% in B. rapa (1151) × B. oleracea (T2). In embryo culture, the hybrid rate was significantly enhanced at 16–18 DAP, up to 48.1% in B. oleracea (T3) × B. rapa (JB2). The combinations of optimal DAP for excision and media components increased recovery of hybrids for ovary and embryo culture,
and constituted an improved technique for B. rapa × B. oleracea crosses. In addition, yellow seeds were obtained from progenies of two crosses, indicating the feasibility of developing yellow-seeded
B. napus through the hybridization between yellow-seeded diploids B. rapa and B. oleracea var. acephala. 相似文献
10.
Canker stain, caused by the fungus Ceratocystis platani, is a destructive disease in Platanus spp. It has been recently proved that resistant accessions can be produced and grown in Europe. However, additional resistant
genotypes are still needed in order to avoid the onset of virulent pathogen strains favoured by the selection pressures exerted
by genetically homogeneous resistant plane tree plantings. In this study we present the results of two parallel experiments
performed on 975 accessions of P. × acerifolia seedlings and P. × acerifolia clones derived by cutting propagation from mature trees grown in the urban environments. The selection process was based
on inoculation with C. platani and yielded 13 accessions that showed different types of resistant reactions and survived in a stable manner thoughout the
period of observation. Selected accessions were characterised by sequencing the rDNA-ITS region and by developing PCR procedures
capable of detecting P. orientalis and P. occidentalis
LEAFY homoeologues. These molecular analyses enabled us to confirm the identification of the species, its hybrid origin and to
assess an evident genetic variability among the accessions, which therefore have to be considered as different genotypes.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
11.
Rice is mainly a self-pollinating crop, but some outcrossing has been reported. Outcrossing with an undesirable donor would lead to the creation of segregants or off-types, which would adversely affect genetic purity and uniformity of the crop. Outcrossing rates in rice under field conditions were investigated using cultivar WAB96-1-1 as a pollen donor and WAB56-104, NERICA 2, NERICA 4 and NERICA 7 as pollen recipients. Levels of outcrossing were investigated up to 30 m from the pollen donor. Dominant morphological markers of red kernel colour and pubescent leaves of the donor were used to identify hybrids. A total of 721 134 plants were investigated. There was an average outcrossing rate of 0.7 ± 0.51%, with a potential outcrossing rate of 2.45 ± 0.86%. Outcrossing rates decreased with increase in distance. It ranged from 2.45% at 0.2 m from the donor to 0.05% at 25 m from the donor. Differences were observed between genotypes and seasons. In season 1 the highest average outcrossing rate of 1.2 ± 0.63% was with WAB56-104 and in season 2 it was 1.1 ± 0.69% with NERICA 4. Outcrossing occurred up to 30 m from the donor. This has implications for germplasm management and conservation and the production of high quality seed. Spatial isolation remains the most practical method to prevent undesirable gene flow. The study indicated that red kernel colour and leaf pubescence can be used to effectively assess outcrossing under field conditions in rice. 相似文献
12.
Halima Benbouza George Lognay Jodi Scheffler Jean Pierre Baudoin Guy Mergeai 《Euphytica》2009,165(2):211-221
The expression of the “glanded-plant and glandless-seed” trait was assessed using High Performance Liquid Chromatography (HPLC) analysis methods in different Gossypium hybrids obtained by crossing Australian diploid cottons and various diploid and tetraploid species. Significant variation
in the gossypol content in the seed was observed among the analyzed genotypes. HPLC data demonstrated that the gossypol synthesis
repression mechanism in the Australian diploid species belonging to C and G genomes was dominant but did not confirm its preferential
functioning against A genome species bearing GL2 locus. About 10% of the produced seeds had total gossypol content lower than the limit imposed by the World Health Organisation
(600 ppm) for the use of cotton flour in food and feed. HPLC analysis of the terpenoids aldehyde (TA) contents in the aerial
parts of the hybrids showed important qualitative and quantitative variability. This result could indicate a certain separation
between pigment gland morphogenesis and terpenoid synthesis mechanisms in cotton. 相似文献
13.
Yan Liang Kunpu Zhang Liang Zhao Bin Liu Qingwei Meng Jichun Tian Shijie Zhao 《Euphytica》2010,171(1):145-156
Dry matter accumulation (DMA) and photosynthetic capacity are important traits that influence biological yield and ultimate
grain yield in wheat. In this study, quantitative trait loci (QTLs) analyses for DMA of stem, leaves, total plant and photosynthesis
traits (Fv/Fm) at the jointing and anthesis stages were studied, using a set of 168 doubled haploid lines (DHLs) derived from
the cross Huapei 3 (HP3)/Yumai 57 (YM57). QTL analyses were performed using QTL-Network 2.0 software based on the mixed linear
model approach. A total of 18 additive QTLs and 12 pairs of epistatic QTLs were distributed on 16 of the 21 chromosomes. Most
of the additive QTLs associated with DMA co-located in the same or adjacent chromosome intervals with QTLs for grain yield
and related traits. A major locus Qculmc.sau-5D.1 (14.2%) close to the molecular marker Xwmc215 detected at the jointing stage was shared by QTLs for heading date and vernalization sensitivity, indicating tight linkages
or pleiotropisms. One pair of epistatic QTLs, Qleavesc.sau-4A and Qleavesc.sau- 6B, explained 13.11% of the phenotypic variation at anthesis. All QTL × environment interactions were detected at the jointing
stage, showing the importance of the jointing stage in determining the final outcome of plant development. 相似文献
14.
The nucleotide-binding site (NBS)-leucine-rich repeat (LRR) gene family comprises the largest number of known disease resistance (R) genes and is one of the largest gene families in plants. In the present study, the full-length cDNA of ZmNL (GenBank Accession Number KF765443) was isolated using Rapid Amplification of cDNA Ends. The nucleotide sequence of ZmNL contains an open reading frame of 3156 bp that encodes the ZmNL protein, which is comprised of 1051 amino acid residues. This putative protein has high homology to other known resistance proteins (84% to Triticum aestivum LR10) and belongs to the CC–NBS–LRR type R gene family. The ZmNL gene was introduced into the maize inbred line of Huangzao4 which was highly susceptible to head smut under the control of the maize ubiquitin promoter by Agrobacterium-mediated transformation. The head smut disease incidence of 3 T2 transgenic lines was significantly reduced (by 18.38–29.40%) compared with the wild type, which indicated that the overexpression of ZmNL gene in maize enhanced the resistance to the fungus Sporisorium reilianum (Kühn) Clint of these plants. 相似文献
15.
In order to elucidate the cytological mechanism of 2n pollen formation in Chinese jujube, a cultivar named ‘Linglingzao’ (2n = 2x = 24) which produces relative more 2n pollens naturally was employed for microsporogenesis analysis. Chromosomes paired in 12 bivalents at diakinesis and the first
meiotic division was normal, whereas, the second division was characterized by frequent abnormal spindle orientation (parallel
and tripolar spindles). Perpendicular, tripolar, and parallel spindles at metaphase II accounted for 72.93, 17.22, and 9.85%
respectively. Perpendicular and tripolar spindles led to tetrads and triads formation respectively. Two types of parallel
spindles were observed and a significant association correlation (r = 0.84, P < 0.05) between parallel spindle II (fused spindles) and dyads was found which meant only fused spindles can form dyads,
while parallel spindle I with a long distance between two sets of chromosomes seemed to lead tetrad formation. At tetrad stage,
the observed frequencies of tetrads, triads and dyads were 80.99, 16.40, and 2.61% respectively. By the rule that each tetrad
can form four n pollens, each triad can form one 2n pollen and two n pollens, each dyad can form two 2n pollens, the frequency of 2n pollen estimated was 5.71%. Based on the pollen diameter difference between n and 2n pollens, frequency of 2n pollen observed was 6.15%. No significant difference was observed between frequency of 2n pollen estimated and that of observed. The results indicated that, tripolar spindles and fused spindles at metaphase II followed
by more triads and few dyads formation at tetrad stage were responsible for the production of 2n pollen in Chinese jujube. 2n gametes observed correspond to first division restitution. Research on the cytological mechanism of unreduced pollen will
provide a platform for unreduced pollen induction and polyploidy breeding in Chinese jujube. 相似文献
16.
D. Meijer M. Viquez-Zamora H. J. van Eck R. C. B. Hutten Y. Su R. Rothengatter R. G. F. Visser W. H. Lindhout A. W. van Heusden 《Euphytica》2018,214(7):121
Usually, mapping studies in potato are performed with segregating populations from crosses between highly heterozygous diploid or tetraploid parents. These studies are hampered by a high level of genetic background noise due to the numerous segregating alleles, with a maximum of eight per locus. In the present study, we aimed to increase the mapping efficiency by using progenies from diploid inbred populations in which at most two alleles segregate. Selfed progenies were generated from a cross between S. tuberosum (D2; a highly heterozygous diploid) and S. chacoense (DS; a homozygous diploid clone) containing the self-incompatibility overcoming S locus inhibitor (Sli-gene). The Sli-gene enables self-pollination and the generation of selfed progenies. One F2 population was used to map several quality traits, such as tuber shape, flesh and skin color. Quantitative trait loci were identified for almost all traits under investigation. The identified loci partially coincided with known mapped loci and partially identified new loci. Nine F3 populations were used to validate the QTLs and monitor the overall increase in the homozygosity level. 相似文献
17.
Chun-Jie Wang Lian-Quan Zhang Shou-Fen Dai You-Liang Zheng Huai-Gang Zhang Deng-Cai Liu 《Euphytica》2010,175(3):323-329
It is believed that unreduced gametes with somatic chromosome numbers play a predominant role in natural polyploidization.
Allohexaploid bread wheat originated from spontaneous hybridization of Triticum turgidum L. with Aegilops tauschii Coss. Unreduced gametes originating via meiotic restitution, including first-division restitution (FDR) and single-division
meiosis (SDM), are well documented in triploid F1 hybrids of T. turgidum with diploid Ae. tauschii (genomic constitution ABD, usually with 21 univalents in meiotic metaphase I). In this study, two T. turgidum lines known to carry genes for meiotic restitution were crossed to tetraploid Ae. tauschii. The resulting F1 hybrids (genomes ABDD), had seven pairs of homologous chromosomes and regularly formed 14 univalents and seven bivalents
at metaphase I. Neither FDR nor SDM were observed. The distribution of chromosome numbers among progeny obtained by self pollination
and a backcross to T. turgidum showed the absence of unreduced gametes. These results suggest that high homologous pairing interfered with meiotic restitution
and the formation of unreduced gametes. This may be related to asynchronous movement during meiosis between paired and unpaired
chromosomes or to uneven distribution of chromosomes in anaphases, resulting in nonviable gametes. 相似文献
18.
Ibrahim Wasiu Arolu Mohd Y. Rafii Marhalil Marjuni Mohamed M. Hanafi Zulkefly Sulaiman Harun A. Rahim Mohd Isa Zainol Abidin Mohd Din Amiruddin Ahmad Kushairi Din Rajanaidu Nookiah 《Euphytica》2017,213(7):154
In practice, progeny and individual palm selection are believed to be the most suitable breeding approach for improvement of quantitative traits in oil palm because their phenotypic expressions are strongly influenced by abiotic factors. Therefore progeny selection approach was applied in this study for the selection of high fresh fruit bunch (FFB) and dwarf oil palm planting materials. Cross between Deli dura and Nigerian pisifera resulted into 34 D × P full sib progenies with 1036 seedlings. For six consecutive years, data were collected on yield and yield component traits, while vegetative traits were recorded once. Bi-parental analysis was carried out using analysis of variance, followed by progenies mean comparison, variance components, heritabilities and cluster analysis. Highly significant (P ≤ 0.01) progeny effect was recorded in this study and this had a pronounced effect on the expression of all the quantitative traits. Progenies performance of FFB varied significantly and it ranged from 166.49 to 220.06 kg/palm/year (kg/p/yr) with trial mean of 192.93 kg/p/yr. Palm height after 8 years of field planting ranged from 1.67 to 2.78 m (control cross) with trial mean of 2.12 m. Broad sense heritability (\({\text{h}}_{\text{B}}^{2}\)) was found to be very low (<17.60%) for all the yield traits, however this parameter was high for vegetative traits with palm height having \({\text{h}}_{\text{B}}^{2}\) of 90%. Cluster analysis based on all the quantitative traits grouped all the 34 DP progenies into nine distinct clusters. From this study, five progenies (DP3, DP4, DP5, DP8 and DP24) were identified to be high yielding and dwarf palms compare to trial mean. At density of 140 palm/ha, they will produce FFB of 28.63–30.81 t/ha and average of 29.69 t/ha which is about 27.15% higher in FFB when compared to the current planting material with FFB of 23.35 t/ha. In addition, the selected progenies possessed average annual palm increment of 29.82 cm/yr with range of 26 and 32.5 cm/yr which was 57.33% shorter than the current planting material with palm height increment of 45–75 cm/yr. 相似文献
19.
Squash silverleaf (SSL), caused by the silverleaf whitefly [Bemisia argentifolii (formerly known as Bemisia tabaci Gennadius, B strain)], is an important physiological disorder that affects squash (Cucurbita spp.) by reducing yield potential. Breeding squash with resistance to SSL disorder can be facilitated by using marker-assisted
selection (MAS). Resistance to SSL disorder, in Cucurbita pepo, is conferred by a single recessive gene (sl). The objective of this study was to identify molecular markers associated with resistance. A zucchini squash, SSL disorder
resistant breeding line, ‘Zuc76’ (sl/sl) and a SSL disorder susceptible zucchini cultivar ‘Black Beauty’ (Sl/Sl) were screened with 1,152 randomly amplified polymorphic DNA (RAPD) primers and 432 simple sequence repeat (SSR) markers
to identify polymorphisms. Using F2 and BC1 progeny segregating for SSL disorder resistance, three RAPD (OPC07, OPL07 and OPBC16) primers and one SSR (M121) marker were
found associated with sl. Fragments amplified by RAPD primer OPC07 was linked in coupling phase to sl, whereas RAPD primer OPL07 was linked in repulsion phase. RAPD primer OPBC16 and SSR marker M121 were co-dominant. The allelic
order of these loci was found to be M121–sl–OPC07–OPL07–OPBC16. The closest marker to sl is M121 with an estimated genetic distance of 3.3 cM. The markers identified in this study will be useful for breeding summer
squash (C. pepo) for SSL disorder resistance derived from zucchini squash breeding line ‘Zuc76’. 相似文献
20.
Antonín Dreiseitl 《Euphytica》2018,214(2):40
Genetic resistance is an efficient and environmentally acceptable way of limiting the damaging effects of plant pathogens on yield and quality of crops. Tests of winter barley variety Venezia revealed an unknown resistance to all tested Blumeria graminis f. sp. hordei isolates. Response type arrays (RTAs) obtained here were created using common avirulent (RT 0) isolates and virulent (RT 4) isolates that first appeared in 2011. RTA of Venezia was identical to RTAs of six other varieties, but differed from RTAs of all other previously tested varieties. Venezia was the first variety to be registered with this resistance, and it is recommended that the resistance be designated Ve. Among 905 isolates randomly collected from the Czech aerial pathogen populations from 2009 to 2015, 13 contained Ve virulence. Each of the isolates differed from the others and thus belonged to different pathotypes. Seven of these 13 pathotypes were collected in the western region of the Czech Republic in an area close to Germany, where Venezia was grown. This finding could support the hypothesis that pathotypes virulent to Venezia have migrated from Germany into the Czech Republic. 相似文献