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1.
Utilization of silage nitrogen in sheep and cows: amino acid composition of duodenal digesta and rumen microbes 总被引:1,自引:0,他引:1
Two experiments are reported. In the first experiment the amino acid compositions of rumen bacteria and protozoa isolated from sheep given a diet of grass silage were compared with those taken from sheep given a diet of hay and barley; four sheep were assigned to each diet. There were no significant differences (P<0·05) between diets in the amino acid compositions of the microbial fractions and bacterial contents of α-ɛ-diaminopimelic acid were also similar for both diets.
In the second experiment the digestion of a grass silage and barley diet (65:35; 135 g CP (kg DM)-1 ) was studied using four non-lactating Ayrshire cows fitted with cannulae in the rumen and in the proximal duodenum. The mean N intake was 154 g d-1 and the corresponding duodenal flow was 126 ± 10 g d-1 indicating that 0·21±0·07 of the N intake was absorbed between the mouth and the duodenum. The mean rumen NH3 -N concentration was high, 297 g litre-1 , and the mean rate of microbial protein synthesis in the rumen was low, 17 g microbial N (kg OM apparently digested in the rumen)-1 . There was a low content of bacterial N in the non-ammonia N at the duodenum (mean proportion 0·52±005) and low concentrations of methionine and lysine in the duodenal digesta protein. The results are discussed in relation to previously published data on the digestion of silage diets in sheep and to the utilization of silage diets for milk production in the cow.
It is concluded that with silage diets the supply of methionine and lysine to the duodenum is likely to be low because of the low rates of microbial protein synthesis in the rumen and that in cows those two amino acids may be limiting for milk production. 相似文献
In the second experiment the digestion of a grass silage and barley diet (65:35; 135 g CP (kg DM)
It is concluded that with silage diets the supply of methionine and lysine to the duodenum is likely to be low because of the low rates of microbial protein synthesis in the rumen and that in cows those two amino acids may be limiting for milk production. 相似文献
2.
Enzymes as silage additives. 1. Silage quality, digestion, digestibility and performance in growing cattle 总被引:5,自引:0,他引:5
Four grass silages were made from perennial ryegrass ensiled after a 1h wilt in 2-t silos without additive application, with application of formic acid or with one of two enzyme mixtures of hemicellulases and cellulases (enzyme 1 and enzyme 2). Effluent losses were monitored over the ensiling period (130 d).
Analyses of the silage showed that formic acid-treated silage had lower concentrations of lactic acid than the other silages. Both enzyme-treated silages had lower levels of cellulose, acid detergent fibre (ADF) and neutral-detergent fibre (NDF) than the untreated and formic acid treated silages. Effluent production was highest with enzyme-treated silages.
The silages were subsequently fed to growing steers equipped with rumen cannulae and T-piece duodenal cannulae. Apparent whole-tract digestibilities of organic matter constituents were significantly lower ( P < 0·05) with both enzyme-treated silages (untreated; 0·736, formic acid; 0·722, enzyme 1; 0·694, enzyme 2; 0·703). Both untreated and enzyme 2-treated silages sustained higher nitrogen digestibilities (g g−1 intake) (untreated; 0·675, formic acid; 0·636, enzyme 1; 0·630, enzyme 2; 0·662) and N retentions (g d−1 ) untreated; 16·0, formic acid; 14·0, enzyme 1; 11·6, enzyme 2; 16·6), but none of these differences was significant. When formic acid-treated silage was offered, there was a greater amount of organic matter apparently digested in the rumen (ADOMR). Non-ammonia nitrogen and microbial nitrogen flows at the duodenum were similar on all diets. The efficiency of microbial protein synthesis was highest with enzyme 2-treated silage and lowest with formic acid-treated silage (untreated, 35·4; formic acid, 25·2; enzyme 1, 30·4; enzyme 2, 39·4), but none of these differences were significant. 相似文献
Analyses of the silage showed that formic acid-treated silage had lower concentrations of lactic acid than the other silages. Both enzyme-treated silages had lower levels of cellulose, acid detergent fibre (ADF) and neutral-detergent fibre (NDF) than the untreated and formic acid treated silages. Effluent production was highest with enzyme-treated silages.
The silages were subsequently fed to growing steers equipped with rumen cannulae and T-piece duodenal cannulae. Apparent whole-tract digestibilities of organic matter constituents were significantly lower ( P < 0·05) with both enzyme-treated silages (untreated; 0·736, formic acid; 0·722, enzyme 1; 0·694, enzyme 2; 0·703). Both untreated and enzyme 2-treated silages sustained higher nitrogen digestibilities (g g
3.
The effect of cell wall degrading enzymes or formic acid on fermentation quality and on digestion of grass silage by cattle 总被引:1,自引:0,他引:1
A first cut of timothy, treated with water (untreated), formic acid (FA), cellulase + lactic acid bacteria (CB), cellulase + hemicellulase (CH) or cellulase + hemicellulase + a lignin-modifying enzyme (CHL), was ensiled in pilot-scale silos. Silages, except CB, were fed to four male cattle, each equipped with a rumen and duodenal cannula, in a digestibility trial designed as a 4 × 4 Latin square. The animals were fed a diet of 400 g of concentrate and 600 g of silage at a level of 70 g DM kg?1 live weight (LW0·75). All enzyme-treated silages were well-preserved with a more extensive fermentation than in FA silage. The quality of untreated silage was poorer as indicated by higher pH and ammonia-N content. The amount of effluent from enzyme-treated silages ranged from 116 to 127 g kg?1; for FA and untreated silages values were 101 g kg?1 and 80 g kg?1, respectively. Total DM losses from enzyme-treated silages were higher than from FA silage (P < 0·05). No significant differences were noticed between silages in the apparent digestibility of organic matter (OM), neutral-detergent fibre (NDF), acid-detergent fibre (ADF) or nitrogen (N). The apparent digestibility of cellulose was higher with enzyme-treated silages than with FA silage (P < 0·05). The values for microbial N flow at the duodenum were 80·0, 91·9, 80·7 and 70·5g N d?1, and for the efficiency of rumen microbial N synthesis 38·6, 47·6, 36·9 and 32·5 g N kg?1 OM apparently digested in the rumen for untreated, FA, CH and CHL silages, respectively. In the rumen the molar proportion of propionate was higher (P < 0·01) and that of butyrate lower (P < 0·01) with enzyme-treated silages when compared with FA silage. The proportion of butyrate was also lower with untreated than with other silages (P < 0·01). The rumen residence time of NDF and ADF was longer (P < 0·05) with enzyme-treated silages than with FA silage. 相似文献
4.
Degradation of nitrogenous compounds in conserved forages in the rumen of dairy cows 总被引:1,自引:0,他引:1
Degradation in the rumen of dairy cows of nitrogenous compounds in 35 grass silages and 12 grass hays was studied by means of the nylon bag incubation technique. Silages varied in dry matter content, crude protein content and date of harvesting; hays varied mainly in crude protein content. Measurements in both forages were washable (W), degradable (D) and undegradable fraction (U) and rate of degradation (ks) of D. W and U were measured as the residue after washing without incubating (W) or after 336 h in the rumen (U). N escape (E) was calculated from these data, assuming a ruminal passage rate of 4·5% per hour.
E (N escape from the rumen) in hays was substantially higher than in silages, mainly because a large proportion of N compounds in silage became soluble during the ensiling process. Of the total variation in E, around 80% could be explained in both forages. In silage, E was positively influenced by dry matter content, negatively by crude protein content and positively by the number of days elapsed since 1 April. This effect of day of harvesting was mainly through an increase of U and may not be beneficial to the animal. In hays crude protein content was the major factor explaining variation in E. 相似文献
E (N escape from the rumen) in hays was substantially higher than in silages, mainly because a large proportion of N compounds in silage became soluble during the ensiling process. Of the total variation in E, around 80% could be explained in both forages. In silage, E was positively influenced by dry matter content, negatively by crude protein content and positively by the number of days elapsed since 1 April. This effect of day of harvesting was mainly through an increase of U and may not be beneficial to the animal. In hays crude protein content was the major factor explaining variation in E. 相似文献
5.
E. Charmley 《Grass and Forage Science》2002,57(1):11-18
Nutritive value and voluntary intake of legumes are generally considered to be higher than those of grasses when ensiled at similar digestibility, although high levels of soluble protein can result in low N utilization by animals and high losses to the environment. The objectives of this experiment were to describe the optimum combination of Westerwolds ryegrass ( Lolium multiflorum Lam. cv. Aubade) and lucerne ( Medicago sativa L. cv. AC Caribou) silages to maximize liveweight gain of steers fed silage, determine chemical components that are important and ascertain whether steers selected the optimum mixture when given a choice. Both silages contained similar concentrations of dry matter (DM), acid-detergent fibre (ADF) and organic acids, but lucerne silage had higher concentrations of N, soluble-N and ammonia-N. Westerwolds ryegrass silage contained more neutral-detergent fibre (NDF). In a 12-week experiment, voluntary intake by Hereford steers was not influenced when the proportion of the two silages was changed from 1 to 0 in 0·25 increments. However, liveweight gain and feed efficiency increased linearly ( P < 0·001) as the proportion of ryegrass silage fed was increased. When preconditioned to either of the two silages, steers showed a significant preference for ryegrass over lucerne ( P < 0·05). When conditioned to a mixture of both silages, no preference was elicited. It is suggested that extensive solubilization and deamination of protein in the lucerne silage may have caused the preference for Westerwolds ryegrass silage and the higher liveweight gains on diets containing higher proportions of Westerwolds ryegrass silage. 相似文献
6.
Protein supplementation of formic acid- and enzyme-treated silages. 2. Nitrogen and amino acid digestion 总被引:1,自引:0,他引:1
Two silages were made from primary growth perennial ryegrass and ensiled after the application of either formic acid or an enzyme mixture of cellulase and hemicellulase. Silage analysis showed both silages to be well preserved with low pH of 3·70 and 3·62 for the formic and enzyme treatments respectively. Formic acid-treated silage had a higher total amino acid concentration than enzyme-treated silage. The silages were offered to growing steers either as the sole diet or supplemented with rapeseed meal at 60 g or 120 g fresh weight kg−1 silage DM offered, in a 6 × 6 Latin square arrangement.
Non-ammonia nitrogen and microbial nitrogen flows at the duodenum (g d−1 ) were significantly ( P < 0·05) increased by supplementation of enzyme-treated silage compared with formic acid-treated silage (enzyme, 83·6, 58·7; enzyme + 60 g, 101·7, 75·3; enzyme + 120 g, 112·5, 80·7; formic, 91·9, 63·7; formic + 60g, 88·3, 67·9; formic + 120 g, 95·5, 67·1) respectively. Efficiencies of microbial protein synthesis were increased for supplemented enzyme-treated silage diets and values were reduced for supplemented formic acid-treated silage diets compared with the silage only diets (enzyme, 27·9; enzyme + 60 37·7; enzyme + 120 g, 38·6; formic, 33·7; formic + 60g, 31·2; formic + 120 g, 28·8). Total amino acid flow at the duodenum increased with supplementation of both silages; however, microbial amino acid flow increased significantly ( P < 0·05) with supplementation of enzyme-treated silage compared with formic acid-treated silage diets. Significantly greater amounts of cystine, methionine, alanine, valine and aspartic acid entered the small intestines of animals receiving supplemented enzyme silages compared with supplemented formic acid silages. 相似文献
Non-ammonia nitrogen and microbial nitrogen flows at the duodenum (g d
7.
Silages were made from the first cut of a predominantly perennial ryegrass sward. The silages were either untreated (W) or treated with formic acid (31 t−1 , F) or with 106 lactic acid bacteria (LAB) g−1 grass of each of three strains alone (A. Lactobacillus plantarum MTD1; B, Pediococcus species 6A2; C, L. plantarum 6A6) or in combination (AB. AC) to give seven treatments. The silage fermentation in 10-kg silos was followed chemically and microbiologically and the nutritive value of selected treatments evaluated using 2-t silos.
The control silage (W) fermented well. Addition of formic acid restricted fermentation and produced a silage with a high ethanol concentration. After day 4, all inoculated silages had lower pH values and higher lactic acid concentrations and a higher ratio of lactic acid to acetic acid than the control silage. Chemically there was little difference between the inoculated silages in terms of final composition. Microbiologically the LAB applied in treatments B and C dominated the LAB populations in those silages when applied alone; however, they were suppressed when applied in combination with inoculant A.
When fed to sheep, the intake of the formic acid-treated silage was significantly ( P < 0·01) lower than that of the other silages and the intake of silage treated with inoculant A significantly ( P < 0·001) higher than that of silages treated with inoculants B and C. The apparent organic matter ( P < 0·001) and nitrogen ( P < 0·01) digestibilities of the formic acid-treated silage were also significantly lower than those of the other silages. 相似文献
The control silage (W) fermented well. Addition of formic acid restricted fermentation and produced a silage with a high ethanol concentration. After day 4, all inoculated silages had lower pH values and higher lactic acid concentrations and a higher ratio of lactic acid to acetic acid than the control silage. Chemically there was little difference between the inoculated silages in terms of final composition. Microbiologically the LAB applied in treatments B and C dominated the LAB populations in those silages when applied alone; however, they were suppressed when applied in combination with inoculant A.
When fed to sheep, the intake of the formic acid-treated silage was significantly ( P < 0·01) lower than that of the other silages and the intake of silage treated with inoculant A significantly ( P < 0·001) higher than that of silages treated with inoculants B and C. The apparent organic matter ( P < 0·001) and nitrogen ( P < 0·01) digestibilities of the formic acid-treated silage were also significantly lower than those of the other silages. 相似文献
8.
Effects of extended wilting, shading and chemical additives on the fatty acids in laboratory grass silages 总被引:4,自引:0,他引:4
This work investigated the range of fatty acid concentrations in grass silages made from the regrowth of perennial ryegrass Lolium perenne using different techniques involving combinations of shading of the crop before cutting, wilting and the use of chemical additives. The effects of the different silage additives on overall fermentation were large, with many of the formic acid and formalin-treated silages having a very restricted fermentation. Nonetheless, effects on levels and proportions of fatty acids were numerically small. The major differences between silages were generated during field operations (shading and wilting), with little further changes in fatty acids within the silage clamp. The extended wilt had the most dramatic effect on fatty acids with a marked reduction in both total fatty acids (24·6 vs. 17·5 g kg−1 dry matter; s.e.d. = 0·65, P < 0·001) as well as in the proportion of total fatty acids as α-linolenic acid (C18:3 n -3; 0·55 vs. 0·48; s.e.d. = 0·013, P < 0·01). Shading the grass with a black plastic sheet for 24 h before cutting had a similar effect. 相似文献
9.
In three separate feeding experiments using a total of twenty-six individually-housed Ayrshire cows, three wilted silages made from Blanca white clover were offered ad libitum with either different supplements or different proportions of grass silage. The clover silages contained 680 g white clover kg−1 on a DM basis, and had a mean DM concentration of 263 g kg−1 with 231 g CP kg−1 DM and 91 g ammonia-N kg−1 N. The pH values averaged 4·16 and the DOMD concentrations 611 g kg−1 . In experiment 1 the daily intake of clover silage given alone was 15.2 kg DM per cow, i.e. 30·1 g kg−1 live weight, and decreased by 0·76 kg DM kg−1 barley DM and by 0·66 kg DM kg−1 barley plus soybean meal DM when these feeds were offered as supplements. Milk yield and fat concentration were higher on the supplement treatments than on the clover silage-only treatment. In experiments 2 and 3 the intakes of silage and total DM increased as the weight of clover in the diet increased from 0 to 700 g kg−1 with parallel increases in milk yield. The effects on milk composition were small and generally non-significant. Although white clover silages with excellent fermentations were made, it is concluded that the main role of white clover in a silage system will be in mixed swards with grass to reduce the input of fertilizer N and to increase the voluntary intake of silage. 相似文献
10.
Timothy–meadow fescue herbage was ensiled with formic acid (FA) (expressed as 100% solution) at the rates of 0, 2, 4 or 6 L t?1. The silages were fed along with concentrates to bulls fitted with cannulae in the rumen and duodenum. The ration comprised grass silage (700 g kg?1), barley (240 g kg?1) and rapeseed meal (60 g kg?1). The application rate of FA had no effect on the site or extent of the digestion of dietary organic matter (OM) and neutral‐detergent fibre. The flow of total N at the duodenum increased linearly (P < 0·05) with application rate of FA, reflecting mainly an increased (P < 0·01) flow of microbial N. The apparent efficiency of net microbial protein synthesis in the rumen increased (P < 0·05), the proportion of propionate in the volatile fatty acids (VFA) in the rumen was not affected (P > 0·05) but that of butyrate increased (linear and quadratic effects, P < 0·01) with increasing rate of FA. It is concluded that an increase in the rate of FA at ensiling leads to a higher utilization of energy and/or protein‐yielding substrates for rumen microbes and to a modified rumen VFA pattern with an increased proportion of butyrate. 相似文献
11.
The aim of the experiment was to assess the effect of applying, at commercially recommended rates, formic acid or formic acid/formaldehyde at ensilage upon the subsequent digestion of the silages by cattle. Three wilted grass silages were made from perennial ryegrass ensiled at a DM concentration of 206 g kg−1 after a poor wilting period of 49 h without additive application and with application of 2.5 litres t−1 of formic acid or of 4.5 litres t−1 of a mixture of (gkg−1 ) 500 formic acid, 200 sulphuric acid and 200 formatin giving an application rate of 15 g formaldehyde kg−1 herbage crude protein (N × 6.25).
The silages were fed to cattle equipped with rumen cannulae and duodenal re-entrant cannulae. Results from analyses of silage composition and from the digestion of organic matter and N showed no major differences between silages. The efficiency of rumen microbial N synthesis, the rumen degradability of silage N (determined in vivo or in sacco ) and in vivo digestion of individual amino acids were also unaffected by additive treatment. These results indicate that poor wilting conditions before ensilage restricted the effectiveness of additive treatment; the ineffectiveness of formaldehyde in reducing the rumen degradability of silage N may also have been related to the low rate of formaldehyde application. 相似文献
The silages were fed to cattle equipped with rumen cannulae and duodenal re-entrant cannulae. Results from analyses of silage composition and from the digestion of organic matter and N showed no major differences between silages. The efficiency of rumen microbial N synthesis, the rumen degradability of silage N (determined in vivo or in sacco ) and in vivo digestion of individual amino acids were also unaffected by additive treatment. These results indicate that poor wilting conditions before ensilage restricted the effectiveness of additive treatment; the ineffectiveness of formaldehyde in reducing the rumen degradability of silage N may also have been related to the low rate of formaldehyde application. 相似文献
12.
Influence of bacterial inoculant and substrate addition to lucerne ensiled at different dry matter contents 总被引:12,自引:0,他引:12
Field-wilted lucerne was chopped with a forage harvester at 33 ± 1·5, 43 ± 2·0 and 54 ± 1·8% dry matter, treated and ensiled in laboratory silos during four cuttings in each of two years. Treatments were control (C), sugar addition at 2% of fresh weight (S), inoculum applied at 3 × 105 bacteria g−1 herbage (I), and sugar and inoculum combined (IS). Duplicate silos were opened and analysed after 1, 2, 3, (4 or 5), 7, 14 and 60 d of fermentation. The initial rate of proteolysis of lucerne protein decreased with increasing dry matter (DM) content of the lucerne, and was not influenced by the year, cutting or silage treatment. Inoculation increased ( P <0·05) the rate of pH decline for all silage dry matters, and shortened the lag time prior to pH decline with 33 and 43% dry matter silages. Sugar addition had no effect on rate of pH decline or lag time. Inoculation and sugar addition both lowered final pH, acetic acid, ammonia (NH3 ), free amino acids (FAA) and soluble non-protein N (NPN) in silages ( P <0·01) and increased lactic acid content with 33 and 43% dry matter silages. Only inoculation was beneficial at 54% DM with no difference between I and IS. The influence of forage characteristics (epiphytic lactic acid bacteria, buffer capacity and sugar:buffer capacity ratio) on treatment effectiveness varied with dry matter content. 相似文献
13.
A. P. Ruggia Chiesa G. V. Kozloski L. M. Bonnecarrère Sanchez L. D. Lima L. Oliveira C. J. Härter G. Fiorentini R. L. Cadorin Jr 《Grass and Forage Science》2008,63(2):193-201
The changes in chemical composition of hay of kikuyu grass ( Pennisetum clandestinum ) harvested at 50, 70 and 90 d of regrowth, and its effect on intake, digestibility, fermentation and microbial protein synthesis in the rumen (Experiment 1) and oxygen uptake by portal-drained viscera (PDV) (Experiment 2) were evaluated. The experiments were carried out with Polwarth × Texel crossbreed lambs with a mean live weight (s.e.m.) of 35(3·0) kg housed in metabolic cages. Organic matter (OM), neutral-detergent fibre (NDF) and nitrogen (N) intake, as well as rumen ammonia-N concentration, decreased linearly with age of regrowth ( P < 0·05). Acid-detergent fibre (ADF) and indigestible NDF intakes were similar for all treatments. Apparent digestibility of organic matter (OM), NDF and N, as well as true digestibility of OM, microbial protein synthesis in the rumen, N retention, pH of rumen fluid and sugars, amino acids and peptide concentrations in rumen fluid were similar for all treatments. Age of regrowth also did not affect the kinetics of passage of the particulate phase through the digestive tract nor oxygen uptake by PDV. Heat production by PDV represented an average of 0·039 of digestible energy (DE) intake. Increasing the age of regrowth of kikuyu grass from 50 to 90 d did not affect digestibility nor the efficiency of DE use by PDV tissues of lambs but it reduced the nutritive value of the hay due to a lower intake of OM. Intake of hay appeared to be most limited by the ADF and indigestible NDF concentrations of the hay. 相似文献
14.
In three separate feeding experiments using a total of thirty individually-housed Ayrshire cows three silages made from perennial ryegrass were given ad libitum together with supplements of four different hays in the long form. The in vitro D-values of the silages ranged from 0·298 to 0·283, and the hays from 0·280 to 0·200. The daily intake of hay DM varied from 0·2 to 4·2 kg per cow and was given either without or with a daily maximum of 2·2 kg concentrate DM containing 379–527 g CP per kg DM. On average, 1 kg hay DM decreased silage intake by 0·24 kg DM with a range of 0·21–1·20 kg. The hay supplements had only small and non-significant effects on total DM intake, milk yield and milk composition, but increased the daily intake of drinking water. In three behavioural studies, the eating and ruminating times expressed as min per kg DM did not differ significantly between the various supplement treatments. It is concluded that hay has only a marginal value as a supplement for grass silage, although the hay could serve as a useful 'buffer' feed if the amount of silage was limited. 相似文献
15.
Two silages were made from perennial ryegrass ensiled without wilting in 2-t capacity silos with the application of either formic acid or an enzyme mixture of cellulases and hemicellulases. Effluent losses were monitored over the ensiling period. Subsequent silage analysis showed that the enzyme-treated silage had higher concentrations of residual water soluble carbohydrate, lactic acid and acetic acid, and lower concentrations of cellulose, ADF and NDF. Effluent production was higher with the enzyme silage (formic acid, 211 1 t?1; enzyme, 2671 t?1). The silages were either offered as the sole diet or supplemented with rapeseed meal at two levels (60 or 120 g fresh weight kg?1 silage DM offered) to growing steers equipped with rumen cannulae and T-piece duodenal cannulae. Apparent whole tract digestibilities for DM, OM, N, ADF and NDF were similar for all diets although nitrogen retention (g d?1) was increased with supplementation of both silages (formic acid, 21·1; formic acid + 60 g, 23·5; formic acid+ 120 g, 28·5; enzyme, 22·6; enzyme + 60 g, 25·8; enzyme+ 120 g, 31·6). Rumen pH, ammonia and total volatile fatty acids patterns were similar. Supplementation increased the amount of organic matter apparently digested in the rumen (ADOMR) with formic acid-treated silage but not with enzyme-treated silage. Liveweight gains were similar for both unsupplemented silages (0·49 kg d?1). These increased to 0·55 and 0·65 kg d?1 for formic + 60 and formic + 120 respectively. Liveweight gains for the corresponding enzyme-treated supplemented diets were 0·81 and 0·91 kg d?1 respectively. Liveweight gains on supplemented enzyme-treated diets were significantly (P < 0·05) greater than those on formic acid-treated diets. 相似文献
16.
Four grass silages, all made in mid-July from second-harvest perennial ryegrass swards, were compared in a 16-week feeding experiment with twelve Ayrshire cows. Two silages were unwilted and two wilted. All the silages received formic acid ('Add-F') at the rate of 3 litres t-1 either with formalin at the rate of 1 litre t-1 or without formalin. The unwilted and wilted silages had mean dry matter (DM) concentrations of 200 and 243 g kg-1 , and in vitro D-values of 0·293 and 0·272 respectively. The silages were offered ad libitum plus 6 kg concentrates per cow per day. The daily intakes of unwilted and wilted silage DM were 10·2 and 9·2 kg per cow respectively on the formic acid treatment, and 10·2 and 9·2 kg on the formic acid + formalin treatment. The mean daily milk yield on the unwilted silage treatments was 19·2 kg per cow which was significantly higher than the yield of 17·2 kg per cow on the wilted silage treatments. The formalin had no significant effect on milk yield. The four silage treatments had small and non-significant effects on milk composition. It is concluded that the unwilted silages, which had excellent fermentation characteristics, were superior to the wilted silages as a feed for dairy cows. 相似文献
17.
Enzymes as silage additives 2. The effect of grass dry matter content on silage quality and performance in sheep 总被引:5,自引:0,他引:5
Eight grass silages were made from two contrasting varieties of perennial ryegrass, four silages each from a late-cut early-maturing (high dry matter, HDM) variety and from an early-cut, late-maturing (low dry matter, LDM) variety. The grass was ensiled without additive (untreated), or with formic acid, or with one of two enzyme mixtures of hemicellulases and cellulases (enzyme 1 and enzyme 2) for a period of 130 d.
Formic acid-treated silage had lower levels of lactic acid at both levels of dry matter than the other silages. Enzyme treatment of grass prior to ensilage resulted in reduced levels of cellulose, acid-detergent fibre and neutral-detergent fibre in LDM silages and lower acid-detergent fibre and neutral-detergent fibre in the HDM silages compared with the corresponding untreated and formic acid-treated silages. Voluntary intakes (g DM d−1 ) of untreated and enzyme-treated silages were significantly ( P <0·01) lower at both digestibilities compared with formic acid-treated silages (LDM: untreated, 982; formic, 1069; enzyme 1, 868; enzyme 2, 937; HDM: untreated, 931; formic, 1027; enzyme 1, 943; enzyme 2, 914). The organic matter, carbohydrate and nitrogen digestibility coefficients of LDM silages were significantly ( P <0·001) higher than those of HDM silages. There were no significant differences in any component digestibility related to silage additive.
Comparison of digestibility coefficients for constituents of the LDM silages fed to sheep or steers showed no differences between species. 相似文献
Formic acid-treated silage had lower levels of lactic acid at both levels of dry matter than the other silages. Enzyme treatment of grass prior to ensilage resulted in reduced levels of cellulose, acid-detergent fibre and neutral-detergent fibre in LDM silages and lower acid-detergent fibre and neutral-detergent fibre in the HDM silages compared with the corresponding untreated and formic acid-treated silages. Voluntary intakes (g DM d
Comparison of digestibility coefficients for constituents of the LDM silages fed to sheep or steers showed no differences between species. 相似文献
18.
Silages were prepared in late September from a mixed crop of perennial ryegrass and white clover with low dry matter and high nitrogen content. Six different treatments were used during ensiling; addition of either molassed sugar beet pulp or rolled barley, at 50 kg and 25 kg (t fresh grass)?1, formic acid at 51 t?1 and no additive (control). All silages were well fermented with low levels of ammonia and pH. The addition of rolled barley or sugar beet pulp increased the dry matter content of the silages incrementally and appeared to assist the retention of nitrogen in the silage. Dry matter intakes of silages with 50 kg of barley or sugar beet pulp tonne?1 were similar to the control silage when fed to wether sheep but at 25 kg t?1, dry matter intake increased by 0·15 with barley and 0·04 with sugar beet pulp compared to the control. The digestibility of dry matter (DM) and organic matter (OM) were significantly higher than the control for silages treated with formic acid or high levels of barley and sugar beet pulp whereas neutral-detergent fibre (NDF) digestibility did not appear to be significantly affected. All treated silages had a significantly higher metabolizable energy (ME) content than the control, and additions of barley or sugar beet increased the ME content. The ME intakes of all treated silages were also significantly greater than those of the control, and the formic acid-treated and low barley silages were the highest. This appeared to be associated with significantly higher energy digestibility and DM intake. Addition of rolled barley during ensilage resulted in greater nitrogen intake, availability and retention compared to additions of sugar beet pulp. The higher retention with barley silages was associated with a significantly lower proportional loss of absorbed nitrogen in urine, which indicated a more efficient utilization of nitrogen. These differences require further study to determine the mechanisms involved in the interactions between the energy source and herbage protein, both during fermentation and in the rumen. 相似文献
19.
Three first-harvest grass silages made from S23 perennial ryegrass cut on 25 May, 13 June and 25 June, and termed early, medium and late silages respectively, were compared in a 16-week feeding experiment with fourteen Ayrshire cows. The early, medium and late silages had D-values of 71·2, 65·0 and 62·5 respectively. The early silage was offered alone, whereas the medium and late silages were supplemented with a concentrate containing 208 g crude protein per kg DM at rates of 2, 3 and 4 kg per 10 kg milk. Silage DM intake was 12·8 kg per cow per d on the early silage treatment, and decreased progressively as concentrate intake increased on the other silage treatments. The mean daily milk yields were 16·0 kg per cow in the early silage treatment, 17·0, 18·4 and 20·4 kg per cow in the medium silage treatments, and 16·8, 18·1 and 20·2 kg per cow in the late silage treatments on the 2-, 3- and 4-kg concentrate treatments respectively. Fat concentration in the milk was not affected significantly by treatment, whereas the CP and SNF concentrations increased progressively and significantly as supplementary feeding increased. From the relationship between milk yield and concentrate intake it was calculated that the medium and late silages required a daily concentrate supplement of 2·1 kg DM per cow to give the same daily milk yields as the early silage. 相似文献
20.
Four grass silages of two chop lengths and two D-values were made from S23 perennial ryegrass, and compared in a feeding experiment with lactating cows and in an eating-behaviour study with non-lactating cows. The median chop lengths were 11·6 and 11·5 mm in the short silages and 18·2 and 19·5 mm in the medium silages. Chop length had negligible effects on D-value and on pH with values of 3·81 and 3·94 on the short and medium silages respectively.
Silage DM intakes were higher on the short than on the medium-chop silages in both experiments except where the high D-value silage was supplemented with concentrates. Milk yields were not affected significantly by chop length with daily values of 19·3 and 19·7 kg per cow on the short and medium treatments respectively with the high D-value silage, and 18·2 and 18·3 kg per cow with the low D-value silage. Chop length had only small and non-significant effects on milk composition.
Eating times expressed as min per kg DM were significantly lower on the short than on the medium-chop silages.
It is concluded that the small differences between the short- and the medium-chop silages were of no economic importance. 相似文献
Silage DM intakes were higher on the short than on the medium-chop silages in both experiments except where the high D-value silage was supplemented with concentrates. Milk yields were not affected significantly by chop length with daily values of 19·3 and 19·7 kg per cow on the short and medium treatments respectively with the high D-value silage, and 18·2 and 18·3 kg per cow with the low D-value silage. Chop length had only small and non-significant effects on milk composition.
Eating times expressed as min per kg DM were significantly lower on the short than on the medium-chop silages.
It is concluded that the small differences between the short- and the medium-chop silages were of no economic importance. 相似文献