Screen of Biocontrol Bacteria of Ginger Wilt by Inhibiting Zones and Root-colonizing Capacity

2003年6月在雅安飞仙关多功乡13个未发病或发病较轻的生姜田块中分离到217个细菌菌株,其中三个(L1、D4、T10)菌株对姜瘟病有强抑菌作用,用利福平标记后在室内的灭菌土和非灭菌土中分别进行定殖能力的测定,结果表明:L1在平板上的抑菌圈不是最大,而最终定殖密度却是最大,而T10抑菌圈最大,定殖能力却不是最大。在灭菌土中比非灭菌土中的定殖能力强。在田间防病试验中,防病效果分别达60.16%(L1)、39.10%(D4)、52.39%(T10)、此外试验结果还表明三个菌株都有不同程度的增产促长作用。     

Elimination of Sulfate Reducing Bacteria by Fe(Ⅵ) in Oil Production Wastewater

采油所产生的废水中产生的硫酸盐还原菌(SRB)可造成注水系统的腐蚀、结垢和阻塞,严重影响原油的开发与生产.以Fe(Ⅵ)作为氧化型杀菌剂杀灭SRB,试验结果表明:Fe(Ⅵ)对SRB具有很强的灭菌能力;Fe(Ⅵ)投加量C＞10 mg/L时就能满足回注水的要求;适宜的接触反应时间为t＞10min;相应地有Ct＞100 mg·min·L-1.     

Distribution of ChAT in the Diencephalon of the One Day Chicken

The distribution of choline-acetyltransferase (ChAT)-like immunoreactive structure in the one day chicken diencephalons was examined by the method of streptavidin-Perosidase (SP) immunocytochemistry. It was found that ChAT immunoreactive neurons and fibers presented in many regions of one day Chicken. Neural structure containing cholinergic neurons with varying density and immunoreactivity were the N. habexularis lateralis and N.habexularis medialis of the epithalamus, the N.rotundus, N.Ovoidalis, N.subrotundus and N.geniculatus lateralis of the thalamus, the hypothalamus, the N.decussationis supraopticae dorsalis of stratathalamus. There were also many varicose and nonvaricose fibers and terminals in the N.geniculatus lateralis and the hypothalamus. The results suggested that the diencephalons of one day chicken is richly innervated by the cholinergic neuron system.     

Fermented Goat Milk and Cow Milk Produced by Different Starters of Lactic Acid Bacteria： Quality Studies

Lactic acid bacteria （LAB） is widely used as culture starters in dairy fermentation. The aim of this study was to investigate the quality of fermented goat milk and cow milk, as well as the viability of LAB in the same products. Fermentations were performed with pasteurized goat milk or cow milk added with skim milk （18% of solids） using three separately different starters; yoghurt starter （a combination of Streptococcus thermophilus FNCC-0040 and Lactobacillus bulgaricus FNCC-0041）, single starter of Lactobacillus acidophilus FNCC-0029 and Lactobacillus casei FNCC-0051. The parameters observed were pH, acidity, nutritional quality including protein, fat and lactose content and product＇s viscosity. Acidity, pH and viability of LAB were also monitored during storage at refrigerated temperature （4 ℃） for 28 days. Results show that the different LAB starters did not affect the pH, acidity, lactose and protein content. Differences on LAB starters affected fat content and viscosity. The highest score of viscosity （30.00 Pa.s ± 7.02 Pa.s） was observed on products fermented by yoghurt starters, followed by products obtained using starter of L. acidophilus （17.7 ±11.4） and L. casei （8.62 ±0.35）. Protein content, acidity, pH and viscosity were not significantly different between products obtained from goat milk and cow milk. Fat content in fermented goat milk was higher （5.03% ±0.62%） than in fermented cow milk （3.52% ±0.37%）, however, lactose content was higher in fermented cow milk （5.16% ±0.40%） than in fermented goat milk （4.53% ±0.35%）. Total LAB concentration in fermented cow milk during storage was 8.03± 0.52 logt0 cfu/mL, while in fermented goat milk was 7.81 loglo cfu/mL ± 0.67 loglo cfu/mL. There was a 10.83% decrease in LAB viability in fermented cow milk and 11.40% in fermented goat milk after 28 days of storage. In conclusion, quality of fermented milk is affected by the starters applied, raw milk source and storage period.     

Studies of the Ripe Characteristics of Pear

The picked pear is often ripened naturally or artificially in ethylene solution to put the pear into market earlier.When Qiuxiang pear are ripened,a great deal of ethylene is produced,the content of soluble solid,total sugar and titrable acidity are increased.The flesh is softened,the skin of fruit turns yellow,and the fruits are of aromatic favor.At differnet harvest time,respiration intensity appears different changing trends;the fruit qualities and the storage period are influenced too.Artificial ripening promotes fruit ripe and has a better effect than natural ripening.And Qiuxiang pear ripened artificially in earlier the middle or late of August can achieve better fruit qualities and higher economic value.     

The Study on the Analysis Method of the Experiment Result of the Partition Compares Method Designing

讨论了间比法设计试验结果常规分析方法所存在的问题,提出了理论对照的概念及计算公式。采用试验结果与理论对照(CK’)直接比较的分析方法,可以有效解决常规分析方法存在的误判问题。     

Analysis of the Chemical Constituents of the Essential Oil from Anli Pear

The essential oil of the whole fruit and the peel of Anli pear (Pyrus Ussriensis Maxim.) grown in the western region of Liaoning Province was extracted through the hydrodistillation method, and was investigated by gas chromatography-mass spectrometry (GC-MS) technique. The yields of the essential oils of Anli pear whole fruit and the peel were 0.073 and 0.36%, respectively. The identification of the volatile compounds was conducted through the commercial National Institute of Science and Technology (NIST) and Wiley mass spectral search program, confirmed by comparing the retention indices with standard values of authentic samples. A total of 7 and 16 components were identified from the essential oils of the peel and the whole fruit, respectively. The predominant constituent of the two kinds of essential oils was butylated hydroxytoluene, which is a typical antioxidant.     

Analysis of the Construction of Electronic Resources in Local University Libraries through the Utilization of Databases——Taking the Library of the Xi＇an University of Science and Technology as an example

通过对西安科技大学两年来数据库使用量统计分析,核算出部分数据库使用成本,对该校馆藏数据库进行了综合评价,分析了数据库利用率的影响因素并对地方高校图书馆电子资源建设提出建议。     

Profile Analysis of the Proteome of the Egg of the High Royal Jelly Producing Bees （Apis mellifera L.）

The protein composition of the egg development in the high royal jelly producing bees （Apis mellifera L.） was investigated. This pioneer study was to separate and quantify the proteins in the egg of the high royal jelly producing worker bees （Apis mellifera L.） by using two-dimensional gel electrophoresis along with their three-day development. The results showed that 160, 195, and 176 proteins, with a wide range of molecular weight （17-80 KDa） and relatively narrow scope of pI （4. 00-8.40） could be detected on day 1, day 2, and day 3, respectively, during the developmental process of the egg. Meanwhile 44 protein spots were constantly detected along with the egg development. Among them 36% were in the uptrend along with the egg development, 14% were in the downtrend, and 39% were of the largest expressed volume on day 2. In addition, the specific proteins were expressed on day 1, day 2, and day 3 （89, 77, and 80, respectively）. Besides the coexistent and specific proteins, 24 proteins were expressed on day 1 and day 2, but silenced on day 3, 49 proteins were expressed on day 2 and day 3, but silenced on day 1, only 3 proteins were expressed on day 1 and day 3, but silenced on day 2. The result indicates that egg development is a sequential and complex gene controlled process, where the eggs of day 2 express the most active proteins. The coexistent proteins suggest that it is conservative and indispensable for this event. These specific proteins suggest that the different developmental stage needs specific proteins to regulate it.     

Investigation on the Effect of Potassium in Retarding the Senescence of Hybrid Rice

Field and pot experiments were carried out to study the effect of potas-sium in retarding the senescence of hybrid rice. There were three treatments:check(K_0), 112.5 kg (K_1), and 225.0 kg (K_2) potassium chloride/ha in fieldexperiment; 5 ppm(K_0), 15 ppm(K_1), 40 ppm(K_2) KCl in pot experiment. The     

Effect of Rapid Cold Hardening on the Cold Tolerance of the Larvae of the Rice Stem Borer,Chilo suppressalis （Walker）

The effects of rapid cold hardening （RCH） on the cold tolerance of the last instar larvae of Chilo suppressalis （Walker） were evaluated for the first time. The discriminating temperature, induction, detection, duration and extent of RCH of the larvae in the laboratory were tested, and the supercooling points （SCPs） and the contents of water and lipid of the larvae after RCH treatment were determined, respectively. The results showed that the discriminating temperature of the larvae was about -21℃. Mean survival rates of the larvae which exposed to either 0 or 5℃ for 2 and 4 h before exposure to the discriminating temperature for 2 h were significantly higher than those of the control groups （P 〈 0.05）. Moreover, the highest survival rate appeared in the larvae after 0℃ for 4 h treatment. The protection against low temperature gained by RCH at 0℃ for 4 h was rapidly lost on return to 28℃. Mean survival rates of RCH larvae were significantly higher than those of non-acclimated （NACC） larvae and acclimation （ACC） larvae when they were exposed to the discriminating temperature for 2 or 4 h （P〈 0.05）. Moreover, the rates of NACC, ACC, RCH and ACC ＋ RCH larvae from 2 to 6 h to the discriminating temperature resulted in a significant decline. The values of SCPs and the contents of lipid of the larvae which exposed to either 0 or 5℃ for 2 and 4 h showed no significant difference at 0.05 level compared to those of the control groups. But the contents of water in the larvae were obviously decreased. Therefore, it could be concluded that RCH could enhance cold tolerance and affect partly physiological and biochemical components of the larvae of C. suppressalis, but the underlying mechanisms needs to be further explored.     

A Study on the Tryptic Hydrolysis of Proteins in Skim Milk and the Characteristics of the Hydrolysate

Limited enzymatic hydrolysis of skim milk by trypsin was investigated in this paper,and it was found that the degree of hydrolysis (DH) of milk proteins had a relationship of DH^-1=a bt^-1 with the time(t) of hydrolysis at low enzyme concentrations some properties of the protein such as NSI in PI or TCA were changed obviously,It was suggested that mosk milk proteins would be degraded into peptides by trypsin.     

Improving Geese Production Performance in ＂Goose-Fish＂ Production System by Competitive Reduction of Pathogenic Bacteria in Pond Water

This study investigated whether competitive reduction of pathogenic bacteria growth in pond water alleviates lipopolysaccharide (LPS) contamination and improves geese production performances in the “go...     

A Study of the Biological Charactor of Bovine－Rvculture in cells and the Antigent glass－pieces of its infected Cells the dete

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