Soil ecological study on dynamics of K,Mg, and Ca,and soil acidity in shifting cultivation in Northern Thailand

Abstract

Soil degradation caused by excessive land use is presently one of the major constraints on sustainable agriculture in the mountainous area of northern Thailand. In order to obtain basic information about soil fertility problems involved in the transition from traditional shifting cultivation to more intensive upland farming, the dynamics of K, Mg, and Ca, and soil acidity in the farming systems of both Karen and Hmong/Thai peoples were investigated. In the fields that lay fallow for more than 5 y, the soils were highly acidic and poor in exchangeable bases, mainly due to the fact that the fallow vegetation rapidly absorbed inorganic bases (K, Mg, and Ca) in the soils. In the fields both under fallow and cropping within 3 y after the slash and burn practice, the high acidity observed in the soils at the fallow stage seemed to be alleviated by ash input with high alkalinity. The aboveground biomass ranged from 9 to 10 t ha^?1 in the 8 y fallow field and the sum of inorganic bases and alkalinity, which were expected to be added to the soils with ash input, ranged from 3 to 4 kmol( + ) ha^?1 or kmol(-) ha^?1 , respectively. In the fields under continuous cultivation for more than 4 y after the slash and burn practice, the subsoils showed a more acidic nature than in the fields immediately after burning. Judging from the high concentrations of inorganic bases in the soil solution from the subsoils, the decrease of the content of exchangeable bases and resulting soil acidification might have proceeded through leaching loss of these bases. Among the exchangeable bases in the soils, Ca and Mg were generally predominant and K occurred as trace. Comparison of the total contents of the bases with the contents of exchangeable ones showed that most of Ca occurred in an exchangeable form while most of K and Mg occurred in the nonexchangeable forms in the soils. Therefore, Ca was likely to be readily depleted along with soil acidification in continuous cultivation.     

Effect of Calcium/Magnesium Ratio in Soil on Magnesium Availability,Yield, and Yield Components of Maize

Abstract

The effect of calcium (Ca)/magnesium (Mg) ratio in the soil on Mg availability, yield, and yield components of maize (Zea mays L.) was investigated both in the greenhouse and the field at eight different Ca/Mg ratios ranging from 1∶1 and 8∶1. In the greenhouse, exchangeable Ca/Mg ratios of 2∶1 to 3∶1 gave optimum yield of maize without Mg‐deficiency symptoms. The Ca/Mg ratios in the soil were positively and significantly correlated with Ca content and negatively and significantly correlated with Mg content. The Ca/Mg ratio in the soil was also positively and significantly correlated with observed Ca/Mg ratio in the maize plant tissue. The Ca/Mg ratio in the soil could be a limiting factor for Mg nutrition of the maize plant. Under field conditions, exchangeable Ca/Mg ratios in the soil showed no significant effects on the Ca and Mg contents of the maize earleaf. However, optimum maize yield was obtained at an exchangeable Ca/Mg ratio of 6∶1.     

Relation between the amounts of Ca,Mg, and k extracted from organic soils and their concentration in onion and alfalfa tissue

Abstract

The objective of this work was to appraise the double acid (0.05N HCl+0.025N H₂SO₄) extraction agent for assessing the availability of Ca, Mg, and K in organic soils. The evaluation was done by determining the relation and interactions between the concentrations of Ca, Mg and K extracted from soils and those found in onion and alfalfa tissues.

The extraction procedure was found to give good relations (r ≥ 0.848^**) between the concentrations of Ca and Mg extracted from soils and those present in onion and alfalfa tissues, though interactions between the amounts of Ca and Mg extracted from soils were found.

A differentiation among soils was found upon relating the amounts of soil extracted K to its concentration in onion and alfalfa tissues. Soil extracted K was shown to be related to its preponderance (K x 100/K Ca Mg) over other extracted bases (r = 0.975^**). A critical preponderance of 11% K was identified for alfalfa. The critical preponderance of K in crops appraises some of the interactions among available soil cations and, consequently, is suggested as an improved approach for predicting crop response to potassium fertilization.     

Tuber yield and allocation of nutrients and carbohydrates in potato plants as affected by limestone type and magnesium supply

Abstract

Magnesium (Mg) is a nutrient that affects the development of plants and is mainly supplied through liming performed to correct soil acidity. By acting on photosynthesis and influencing carbohydrate partitioning in the plant, supplementary Mg supplied through soil or foliar application can increase the yield and quality of potato (Solanum tuberosum L.) tubers. The aim of this study was to evaluate the effect of supplemental Mg fertilization by soil or foliar application on plant nutritional status, tuber yield, and carbohydrate partitioning in potato crops in soil corrected with calcitic and dolomitic limestones. The experiment was carried out in pots under greenhouse conditions with a randomized block design in a 2?×?3 factorial scheme with four replications. Dolomitic limestone application and supplemental Mg fertilization via soil increased the concentrations of this nutrient in potato leaves. Liming with dolomitic limestone reduced the uptake of Ca and K by plants, but supplemental Mg fertilization did not alter the uptake of Ca, Mg or K. Supplemental Mg fertilization did not increase plant growth and tuber yield, even when calcitic limestone was used to elevate the base saturation to 60%; the exchangeable Mg concentration in soil was 9?mmol_c dm^?3, and the Ca:Mg relationship was 3.7. Liming with dolomitic limestone or providing supplemental Mg fertilization did not increase sugar and starch partitioning to the tubers.     

Dryland corn response to tillage and nitrogen fertilization. II. P,K, CA,MG

Abstract

Nitrogen fertilization and tillage practices may influence the availability and uptake of essential plant nutrients other than N. This study was conducted to assess the interactive effects of N rate and timing and tillage practices on uptake and concentration of P, K, Ca and Mg in corn grown under dryland conditions. Potassium accumulations in no till (NT) soils were greater than in conventional till (CT) near the surface and lower than CT in the subsoil. Phosphorus and Ca levels decreased with soil depth, while Mg tended to accumulate in the subsoil. Phosphorus uptake and concentration of 5‐leaf stage corn was increased as tillage intensity decreased. Nitrogen rate at planting increased 5‐leaf P uptake but reduced P concentration; however, by silking no effect of tillage or N fertilization practice on ear leaf P concentration was obtained. Increases in 5‐leaf corn K uptake and concentration as tillage intensity decreased may have reduced Mg and Ca concentrations via cation antagonism. Ear leaf Mg and Ca concentrations were increased by N rate, probably as a result of solubilization of Ca and Mg and improved crop growth. Distribution of essential elements in the soil due to tillage in combination with varying N fertilization practices can influence temporal nutrient uptake, thereby altering plant nutrient diagnosis.     

Nutrient status of the lowbush blueberry,Lac‐Saint‐Jean area,Québec,Canada

Abstract

The lowbush blueberry (Vaccinium angustifolium Ait.) is an important commercial crop of the Lac‐Saint‐Jean area (Quebec, Canada). The major blueberry fields are located on sandy soils relatively poor in available mineral nutrients. The nutrients originate from a thin organic layer found on the top of these sandy soils. The leaf mineral contents (N, P, K, Mg, Ca, Mn, Fe, Cu, Zn and B) were measured in five blueberry fields during 1984 and 1985. Soil pH and soil available P, K, and Mg were also assessed. The results show that the leaf mineral contents are generally adequate. However, K and Zn might be occasionally deficient when compared to the actual established standards. The available Mg in soil was significantly correlated with the leaf Mg concentration. The data also suggest that the increase of the pH following the burn pruning seems to influence the nutrition of this species.     

Doybean growth and composition as affected by K,Ca, and Mg rates and corn rotation

Abstract

Different rates of K, Ca, and Mg were applied to bulklots of Decatur clay loam (pH 5.8) which had been collected from an area under natural vegetation. Nitrogen and P were each applied at the rate of 100 ppm. Soybean (Glycine max L.) and corn (Zea mays L.) were planted to pots in four replications of each treatment. Plants were grown for 6 weeks and subsequently all the pots were re‐planted to soybeans. This crop rotation was repeated until six crops had been harvested from each pot.

Potassium fertilization did not affect soybean growth but increased the dry matter of corn plants. Calcium application affected the growth of neither crop, but Mg addition to the soil reduced the growth of both crops. The composition of the plants generally reflected the available amounts of each nutrient. Additionally, Mg consistently decreased K in soybeans but increased Mn in the two crops. The inclusion of corn in rotation with soybeans resulted in the following effects on the succeeding soybean harvests: more tolerance to high Mg, greater reduction of plant Ca and Mg caused by K application, and lower levels of available K and Ma in soils and soybeans. However, the greater rate of depletion of soil K and Mn under corn rotation did not appear Co affect the dry matter yields of the following soybean plants relative to the plants under the continuous soybean cropping system.     

Characterization of Spatial Variability of Soil Parameters in Apple Orchards of Himalayan Region Using Geostatistical Analysis

ABSTRACT

Knowledge of spatial variability in soil properties is critical for effective nutrient management plan in crop production. Poor productivity of apple orchards in Indian Himalayan Region (IHR) is due to lack of information on the variability of soil properties important for enhancing productivity in the region. The study was conducted in apple orchards with the hypothesis that spatial variability of soil properties is high due to adoption of varied management and passive soil factors. The major objectives of the study were to assess the spatial variability of soil parameters, viz. soil pH, electrical conductivity, soil organic carbon along with plant available soil nitrogen (N), phosphorus (P), potassium (K), exchangeable calcium (Ca), and exchangeable magnesium (Mg) at a regional scale through geostatistical methods. Coefficient of variation (CV) indicated that the fertility parameters varied from medium to high in heterogeneity (CV > 20%). Available N was found critical/medium in 69.6% of soil samples and might be one of the limiting nutrients for crop growth, P and K were in high, and OC in very high range. Significant correlation was found between OC with N; pH with K, Ca and Mg and EC with OC, P and K. The semivariogram parameters indicated that the spatial distribution of soil fertility parameters were inconsistent and showed strong to weak degree of spatial dependence for all parameters. The study highlighted the importance of delineation of soil fertility management zones in the apple growing region as a guide for precise and site-specific nutrient.     

Correlations Between Soil Exchangeable Ca2+, Mg2+, K+ and Foliar Nutrient Concentrations in Mature Biological Olive Groves (Olea europaea L., cv. ‘Chondrolia Chalkidikis’)

Leaf and soil samples were taken and analyzed from two mature biological olive groves (Olea europaea L., cv. ‘Chondrolia Chalkidikis’), in Thessaloniki, Macedonia, Northern Greece, in order to determine the correlations between soil exchangeable cations and foliar calcium (Ca), magnesium (Mg) and potassium (K) concentrations, and the interrelations among leaf nutrients. Τhe nutritional requirements of trees for both biological groves were exclusively based on patent kali supply and nutrient recycling (via pruning material and weed cut recycling). Foliar K, Ca and Mg were positively correlated with soil exchangeable K, Ca and Mg, in the 40–60 cm layer, then in the 20–40 cm layer. Synergistic uptake mechanisms among Ca²⁺, Mg²⁺ and K⁺ probably exist. Leaf N was negatively correlated with foliar K, and positively with leaf Ca, Mg and manganese (Mn). Foliar P was negatively correlated with leaf Ca, Mg and Mn, while foliar Ca was positively correlated with leaf Mg and Mn. Foliar Mg was positively related with leaf Mn. High phosphorus (P) may decrease leaf Ca, Mg and Mn. Enhanced Ca may increase leaf Mg and Mn, while high Mg may also enhance foliar Mn. Finally, based on the determination of foliar nutrient concentrations, the nutritional requirements of olive trees in Ca, Mg, K, P, Fe, Zn were sufficiently (or over-sufficiently) satisfied. However, additional organic fertilization is needed, in order to achieve optimum levels of N, B and Mn (since their foliar concentrations were slightly insufficient). The correlations between leaf and soil exchangeable Ca, Mg and K, as well as among foliar nutrients should be taken into consideration, in order to achieve successful organic fertilization for mature biological olive groves, and to avoid nutritional imbalances and disorders.     

Maize grain production,plant nutrient concentration and soil chemical properties in response to different residue levels from two previous crops

ABSTRACT

The incorporation of previous crop residues in agricultural management benefits soil fertility, crop production, and environment. However, there is no enough information about maximum residue application level without negative effect over next crop yield. To evaluate maize (Zea mays L.) yield under short-time conservation management with incorporation and/or importation of different residue levels, a biannual rotation experiment was conducted in ash volcanic soil in south-central Chile. The experiment consisted of two previous crops, canola (Brassica napus L.) and bean (Phaseolus vulgaris L.), and four levels of residue incorporation (0%, 50%, 100%, and 200% of generated residue; from 0 to 21.4?Mg?ha^?1 for canola and from 0 to 19.0?Mg?ha^?1 for bean). Previous crop species and residue level affected some nutrients concentrations in grain and plant and some soil chemical properties, without effect in maize yield, which averaged 16.6?Mg?ha^?1. Bean residue increased Ca and reduced S in maize plant, increasing soil P, Ca, Mg and K (P?<?0.05). Maize grain Ca content was positively and proportionally affected by canola residue level and negatively and proportionally affected by bean residue level. All canola residue levels increased soil pH and Mg, but the highest level reduced soil S; soil P concentration increased proportionally with bean residue level. The highest bean residue level increased soil S. Different crop and levels of residue did not affect maize yield but did some plant nutrient concentration, and also affected some soil chemical properties.     

攀西烟区紫色土pH值与土壤养分的相关分析

对四川攀西烟区有代表性的30个紫色土样品的pH值、有机质及有效养分含量进行了测定。结果表明，攀西烟区紫色土的pH值在5．13～8．14范围内，有半数左右的土壤偏碱性；有机质、碱解氮、有效锌和有效锰的含量均随pH值的升高而降低，交换性钙的含量随pH值的升高而升高，而pH值与速效磷、速效钾、交换性镁、速效铜、速效硼间的相关性则不显著。烟区紫色土的有机质和碱解氮含量偏低，速效磷、速效钾含量中等，交换性钙、交换性镁含量丰富；部分pH值较高的土壤可能会缺乏铜、锌等微量元素；在不同pH值的土壤上硼营养均严重亏缺，建议生产中增施硼肥。     

Compositional Nutrient Diagnosis in Maize Grown in a Calcareous Soil

ABSTRACT

Preliminary compositional nutrient diagnosis (CND) norms for maize (Zea mays L.) were developed from a small database as means and standard deviations of row-centered log ratios V _X of five nutrients [nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg)] and a filling value R, which comprised all nutrients not chemically analyzed and quantified in 72 leaves of hybrid ‘Pioneer 3044.’ Norms were derived from maize grain yields higher than 7.7 t ha^?1. Principal-component analysis performed using CND nutrient indexes allowed us to identify an antagonism between N and Mg explained by the calcareous nature of the soil and the relevant N fertilization with ammonium (NH₄ ⁺), and a positive interaction between P and K, and P-Ca and K-Ca antagonisms. Maize plants tended to take up lower amounts of N and K and higher amount of Mg in our calcareous soil than the concentrations of these nutrients reported to be optimum.     

Effets des engrais N,P et K sur le rendement et la qualite de l'ensilage ue mais

Abstract

Thirteen fertility trials were made throughout Quebec's corn growing region during 1972–74. These included eleven fertilizer combinations with corn silage as the test crop and were carried out on nine soils. Total dry matter and digestible nutrients (TDN) varied greatly from year to year, though, mean yields increased by 23 and 30 percent respectively with the 50 kg N/ha treatment, compared to control receiving no nitrogen fertilization. However, 150 kg N/ha was required to attain a maximum yield of 1250 kg/ha crude protein. Despite a 0.2 percent nitrate content found in the silage grown on the most northerly site, a 120 kg P/ha combined with 100 kg N and K gave the highest mean TDN production (9580 kg/ha).

Potassium fertilization affected plant K content of corn grown at the most northerly site only, where a 0.5 percent was found with the control on a suit containing low potassium levels. Further, striking increases in Ca and Mg concentrations were observed with corn grown on that soil. However, magnesium concentration ranging from 0.11 to 0.14 percent were found with nine field trials out of twelve. Also, low K:(Ca + Mg) ratios were found on three trials, which were increased with potassium fertilization levels of 150 kg K/ha. Accordingly, it is suggested that uptakes of 200, 48, 200, 30 and 30 kg/ha of N, P, K, Ca and Mg are required for good corn silage crops.     

The effect of Ca and K levels on the herbage yield and nutrient composition of barley plants grown in high Mg soils

Abstract

The effects of Ca and K levels on barley (cv. Johnston) yield were studied in soil media containing high levels of Mg. The dry matter yield of barley decreased with increasing concentrations of Mg in soils, but the decrease was small. Dry matter yield was positively related to concentration of K in the soil. However, additions of fertilizer Ca or K did not increase dry matter yield, indicating that depressed yield associated with high Mg levels was not due to reduced availability of Ca or K. In commercial agriculture, applications of either Ca or K to such soils are unlikely to prove beneficial in increasing crop yields.

Concentrations of Mg in soil solutions of unfertilized soils were lower than levels which were previously shown to reduce crop yield. Additions of N fertilizer increased Mg concentrations to levels which could reduce barley yield     

A rapid method to determine cation exchange capacity and exchangeable bases in calcareous,gypsiferous, saline and sodic soils

Summary

A simple, single‐step extraction with LiEDTA for the estimation of CEC and exchangeable bases in soils has been developed. Multivalent cations are stripped from the soil adsorption sites by the strongly chelating agent EDTA, and are replaced by Li. In soils without CaCO₃ or water soluble salts, exchangeable divalent cations (Ca, Mg) are chelated by EDTA and exchangeable monovalent cations (Na, K) are replaced in a single extraction step using 0.25–2.5 g of soil and 10.0 ml of extractant.

In calcareous soils the CEC can be determined in the same way, but for the extraction of exchangeable Ca and Mg, another separate extraction is needed because dissolution of calcite by EDTA is unavoidable. This extraction is done with as much NaEDTA as needed to extract only exchangeable Ca and Mg in a 1:2 (m/V) soil/alkaline‐50% (V/V) aethanolic solution to minimize dissolution of calcite.

In gypsiferous soils gypsum is transformed into insoluble BaSO₄ and soluble CaEDTA by LiBaEDTA thus avoiding interference of Ca from dissolution of gypsum, which renders the traditional methods for determining CEC unsuitable for such soils. To determine exchangeable Ca and Mg, Na₄EDTA is used as for calcareous soils.

In saline/sodic soils replacement of Na by Li is incomplete but the Na/Li‐ratio at the complex after extraction is proportional to the molar Na/Li‐ratio in the extracts, so that the CEC and original exchangeable sodium (ESP) content can be calculated. Additional analysis of Cl and, if necessary, SO₄ in the extracts of saline soils can be used to correct for the effect of dissolution of the salts on the sum of exchangeable cations.

This new method is as convenient as the recently developed AgTU (silverthiourea), but is better suitable for calcareous and gypsiferous soils.     

Plant Availability of Cadmium in Soils: II. Factors Related to the Extractability and Plant Uptake of Cadmium in Cultivated Soils

Abstract

Eighty four soil samples collected from southeastern Norway were analyzed for Cd by extraction with NH₄OAc, DTPA, NH₄OAc-EDTA, NH₄NO₃, HCl and CaCl₂. The total Cd, pH, exchangeable K and Ca, dithionite-extractable Mn, available P and fine sand (0.2–0.02 mm) contents were the principal factors related to the extractable Cd, with some inter-extractant variations. Cadmium extracted by NH₄NO₃, NH₄OAc, HCl and CaCl₂ decreased with increasing soil pH, but the Cd extracted by all the extractants increased with increasing total Cd, exchangeable K and Ca, available P, and Mn-oxide contents in the soils. The Cd concentrations in plants were significantly related to the extractable Cd, exchangeable Ca and Mg, pH, Mn-oxides and organic matter content.     

Soil development and fertility characteristics of inland valleys in the rain forest zone of Nigeria: Physicochemical properties and morphological features

Abstract

Inland valleys are a widespread topography in West Africa and have significant potential for agricultural development, especially wet rice cultivation. This study investigated the physicochemical and morphological properties of the soils of two inland valleys in Abakaliki and Bende, Southeast Nigeria, where the soils are derived from shale materials, and discusses their agricultural potential as well as the soil-forming process. Particle size analysis suggested that the soils at both sites were fine-silty, fine-loamy or clayey and, thus, would be able to retain a high amount of water. In contrast, the higher content of clay and silt in the Abakaliki soils would enhance much more water retention than the Bende soils. The soils in Abakaliki, except for some subsoil horizons, generally had acidic reactions, low contents of exchangeable bases (Ca, Mg, K and Na) and high amounts of exchangeable acidity (Al and H) for which leaching effects under high precipitation in the area would be implicated. Bray-1 P values in these soils were generally low under such acidic conditions, while organic C and total N were recorded at relatively high levels, in particular at the surface horizons, reflecting large biomass production under a humid climate. The Bende soils showed similar chemical properties to Abakaliki except for relative accumulation of exchangeable bases throughout the profile on the downslope possibly because of the rolling topography. This result suggested that geological fertilization (i.e. afflux of nutrients released during the soil formation in the upland into the lowland) was more beneficial in Bende than Abakaliki. From the findings of the present study, we concluded that soils in both Abakaliki and Bende had good texture for sawah development (leveled and bounded rice field with an inlet and an outlet for irrigation and drainage), but their poor chemical properties would be constraints for agricultural production.     

Effect of soil pH and N fertilization oh uptake and fractions of Ca and Mg in two barley cultivars

Abstract

A study has been made of the amounts of Ca and Mg removed from dry matter of young barley plants by sequential extraction with water, M NaCl, 0.4 M acetic acid and 0.6 M HCl. Two contrasting cultivars were examined and other factors included the effects of the soil pH in which the plants were grown and the form of N‐fertilizer used. The distribution of total Ca and Mg and the different fractions between young and old tissues was also examined.

Water and NaCl between them removed the bulk of the Ca and Mg (between 80 and 90%) and broadly, where uptake of total Ca or Mg increased with increase in soil pH, there was little change in the percentage of either cation appearing in the fractions, indicating a constant removal by the extractants, irrespective of content.

Only about a third of the total Ca was found in the upper half of the plants, against over 60% in the case of Mg and the same distribution was found for the percentages of the water‐soluble and the exchangeable fractions.

Small differences between cultivars and between N forms in respect of fractions and their distribution in the plant were found, but these did not affect the overall general pattern.     

Effect of soil pH and N fertilization on uptake and fractions of Ca and Mg in two barley cultivars

Abstract

A study has been made of the amounts of Ca and Mg removed from dry matter of young barley plants by sequential extraction with water, M NaCl, 0.4 M acetic acid and 0.6 M HCl. Two contrasting cultivars were examined and other factors included the effects of the soil pH in which the plants were grown and the form of N‐fertilizer used. The distribution of total Ca and Mg and the different fractions between young and old tissues was also examined.

Water and NaCl between them removed the bulk of the Ca and Mg (between 80 and 90%) and broadly, where uptake of total Ca or Mg increased with increase in soil pH, there was little change in the percentage of either cation appearing in the fractions, indicating a constant removal by the extractants, irrespective of content.

Only about a third of the total Ca was found in the upper half of the plants, against over 60% in the case of Mg and the same distribution was found for the percentages of the water‐soluble and the exchangeable fractions.

Small differences between cultivars and between N forms in respect of fractions and their distribution in the plant were found, but these did not affect the overall general pattern.     

Changes in potassium availability and other soil properties due to soil ingestion by earthworms

An incubation experiment was conducted to study the changes that occur in potassium availability and other soil properties with ingestion of soil by earthworms. Two soils were used. Raumai soil with high non-exchangeable K and Milson soil with low non-exchangeable K were incubated with two species of earthworm, Aporrectodea caliginosa and Lumbricus rubellus, for 8 weeks. The casts and soil samples were analysed for exchangeable K, Ca, Mg, Na, and H, pH, organic C, and texture. The results indicated that in Raumai soil, the exchangeable K levels of the casts of both earthworm species were significantly higher than for the control soil, the effect being more marked for L. rubellus than for A. caliginosa. In Milson soil, the exchangeable K levels were significantly lower in the casts of both types of earthworm than in the control soil. The nitric acid-extractable K of the soil and casts was not markedly different for either soil type, but available non-exchangeable K values were significantly higher for the casts of L. rubellus from Milson soil than for the noningested Milson soil. In Raumai soil, the exchangeable Ca was higher in the casts of L. rubellus, exchangeable Mg and H were reduced, and exchangeable Na did not change markedly in the cast compared to the control soil. For Milson soil, the casts contained lower exchangeable Ca and H but higher Na and Mg than the control. The casts of both species of earthworm had significantly higher pH values for both soil types. There was no marked difference in the organic C content of the control soil and cast samples for Milson but a reduction in the casts of A. caliginosa for the Raumai soil. Finer fractions increased in the casts of both earthworm species in both soil types.