Partial characterization of digestive proteases of the three‐spot cichlid Cichlasoma trimaculatum (Günter 1867)

Partial characterization of digestive proteases in the three‐spot cichlid Cichlasoma trimaculatum juveniles was conducted. It was determined that there is higher alkaline proteases activity (3.95 ± 0.32 IU mg^?1 protein) compared to acidic proteases (2.01 ± 0.57 IU mg^?1 protein). Optimal temperature for alkaline proteases is 60 °C which resulted in more thermostability to temperature changes. On the other hand, optimal temperature for acidic proteases is 50 °C. Optimal pH for acidic proteases was pH 2, while for alkaline proteases, it was pH 10, which resulted in more stability in relation to pH changes than acidic protease. The use of specific inhibitors and the SDS‐PAGE electrophoresis analysis revealed seven types of bands for alkaline proteases, which make evident the main presence of serine proteases. In acidic proteases, more than 98 g kg^?1 of the activity was inhibited with pepstatin A inhibitor. Therefore, it is evident that C. trimaculatum digestion is composed by acidic and alkaline proteases; thus, it should be considered an omnivorous fish.     

Effect of oxidized fish oil on growth performance and oxidative stress of Litopenaeus vannamei

A 4‐week feeding trial was conducted to determine the effects of oxidized fish oil (OFO, POV: 234.84 meq kg^?1) on growth performance and oxidative stress of Litopenaeus vannamei. Five diets containing various OFO levels (0, 25, 50, 75 and 100 g kg^?1) with the same dietary lipid level were fed to L. vannamei. The results showed that the body weight gain and the specific growth rate of the shrimp fed with 50, 75 and 100 g kg^?1 of OFO diets decreased significantly (P < 0.05), whereas the hepatosomatic index increased significantly (P < 0.05). The malondialdehyde concentrations in the serum and muscle of the shrimp fed with 50, 75 and 100 g kg^?1 of OFO diets were significantly higher than that of the shrimp fed with fresh fish oil (P < 0.05). The total antioxidant competence decreased significantly compared with the control group. Therefore, dietary OFO affects the growth performance and increases the oxidative stress of shrimp.     

Diets supplemented with microalgal biomass: effects on growth,survival and colouration of ornamental fish Hyphessobrycon eques (Steindacher 1882)

This study evaluated the effects of the addition of microalgae (Ankistrodesmus gracilis and Haematococcus pluvialis) to the fish diet in improving the growth and optimal pigmentation (red carotenoid) of Hyphessobrycon eques. The basal mixed diets consisted of a formulated diet, supplemented with dried microalgae biomass of A. gracilis (1.5 g kg^?1) and H. pluvialis (1.5 g kg^?1). The live food diets contained zooplankton was cultured in open ponds, associated with microalgae. All the microalgae were cultured in the laboratory. No mortality was observed with any experimental diets. Fish performance results showed significant differences (P < 0.05) between the basal diet (BD) and the live food diet. The higher weight and total length were observed with mixed diets (BD + H. pluvialis and BD + A. gracilis). The mixed diets promoted more intense values of chroma (Cab*), lightness (L*) and redness (a*) to H. eques. Diaphanosoma birgei (Cladocera) represented more than 32% of zooplankton ingested by ornamental fish in live food (zooplankton and zooplankton + microalgae), and Argyrodiaptomus furcatus (Copepoda) was the species most ingested by H. eques in live food dietary treatment zooplankton. The feeding behaviour observed in the laboratory as well as the food preferences of H. eques was dependent on the zooplankton composition present in the used open ponds. This study showed that diets with microalgae and zooplankton were able to enhance the pigmentation of H. eques, being a good tool to benefit the culture management of this species.     

Effects of organic selenium supplemented diet on growth performance and antioxidant response in Cirrhinus mrigala

The present study was conducted to evaluate the …effect of dietary selenium on growth performance and antioxidant enzymes activity in Cirrhinus mrigala for a period of 45 days. Four numbers of experimental diets were prepared, T_c for Control (0 mg organic selenium/kg of diet), T₁ (2.5 mg organic selenium/kg of diet), T₂ (5.0 mg organic selenium/kg of diet) and T₃ (10 mg organic selenium/kg of diet). The water quality parameters (temperature, DO, pH, conductivity, hardness, ammonia, nitrate and nitrite) were found to be insignificant (p > .05) among all the treatments. Superoxidase dismutase (SOD) was found to be significantly highest in T₂ (6.10 ± 0.07) when compared with the other treatments. Similarly, catalase (CAT) activity in T₁ (13.21 ± 0.13) was significantly (p < .05) different with that of Control, T₂ and T₃. Present study also revealed that 2.5mg organic selenium/kg supplemented diet (T₁) showed highest results in net weight gain (15.55 ± 1.36 g), net weight gain percentage (185.88 ± 8.98%) and SGR (2.35 ± 0.06 g) compared with other experimental diet.     

Effect of different feeds on the growth,survival and reproductive performance of zebrafish,Danio rerio (Hamilton, 1822)

Present experiment was conducted to determine the effect of different feeds with varying protein levels on the growth, survival and reproductive performance of zebrafish, Danio rerio. The control diet (T1) was wild‐collected zooplankton from local fish ponds, while test diets with 350 g kg^?1 protein (T2), 400 g kg^?1 protein (T3) and 450 g kg^?1 protein (T4) were formulated and fed to fish for a period of 210 days. The significantly (P < 0.05) highest mean weight gain and specific growth rate were observed in T1, which were similar with T3 and T4. The significantly (P < 0.05) highest number of egg production per female and relative fecundity were found in T1, followed by T4 and T3 while T2 produced lowest number of eggs. No significant (P > 0.05) differences were observed in brood survival rate, fertilization and hatching rate among the dietary treatments. The highest (P < 0.05) fry survival rate was recorded in T1, followed by T3 and T4. Thus, it is suggested that control diet i.e. mixed zooplankton exhibited better growth, reproductive performance and fry survival rate. However, diet containing 400 g kg^?1 crude protein also gave comparable results in terms of growth, survival and reproductive performance of zebrafish.     

Effects of dietary fish oil replacement by microalgae raw materials on growth performance,body composition and fatty acid profile of juvenile olive flounder,Paralichthys olivaceus

Replacing dietary fish oil with DHA‐rich microalgae Schizochytrium sp. and EPA‐rich microalgae Nannochloropsis sp. for olive flounder (Paralichthys olivaceus) was examined. Three experimental isonitrogenous and isolipidic diets with lipid source provided by 50% fish oil (F50S50), 50% (M50F25S25) and 100% microalgae raw material (M100) respectively were compared with a soybean oil (S100) diet as control. Triplicate groups of olive flounder juveniles (16.5 ± 0.91 g) were fed the experimental diets, and a group was fed the control diets for 8 weeks in a recirculation system. Results showed feed efficiency and growth performance were not significantly changed when fish oil (FO) was totally substituted by soybean oil (SO) or microalgae raw material (MRM). The whole‐body composition, lipid content of liver and muscle, and lipid composition of plasma were not significantly influenced by the total substitution of FO by MRM. The polyunsaturated fatty acids (PUFA) content of muscle and liver declined in fish fed S100 diet, whereas it was not significantly reduced in fish fed M50F25S25 and M100 diets. The total substitution of FO by MRM not only maintained the levels of arachidonic acid, EPA or DHA but also increased n‐3/n‐6 ratio. In conclusion, MRM as the sole lipid source is sufficient to obtain good feed efficiency, growth performance and human health value in olive flounder juveniles.     

Effects of ferulic acid on growth performance,immunity and antioxidant status in genetically improved farmed tilapia (Oreochromis niloticus) fed oxidized fish oil

A study was conducted to characterize the effects of dietary oxidized fish oil on the growth performance, immunity and antioxidant status of genetically improved farmed tilapia (Oreochromis niloticus) and to determine the role of ferulic acid on the oxidative damage induced by the oxidized fish oil. The tilapia (13.73 ± 0.31 g) were fed four experimental diets containing untreated (peroxide value, POV: 2.2 meq/kg) and highly oxidized (POV: 120.6 meq/kg) fish oil either with or without ferulic acid (0 or 400 mg/kg) supplementation for 12 weeks. From the results, the oxidized fish oil treatments increased antioxidant enzyme activities and MDA values but decreased the weight gain and the immunological parameters in tilapia. Meanwhile, the serum biochemical indices were significantly affected by the oxidized fish oil. Besides, the addition of ferulic acid partially counteracted the free radical‐induced damage and improved the health status of tilapia. In conclusion, the oxidized fish oil may induce oxidative stress, destroy liver, dysregulate lipid metabolism as well as reduce non‐specific immunity, and eventually result in growth inhibition of tilapia. The ferulic acid supplementation partially offset the negative effects of the oxidized fish oil on tilapia.     

The investigation of the effects of different feeding strategies on the growth of dolphin cichlid (Cyrtocara moori Boulenger, 1902) fish

This study was designed to investigate the effects of restrictive and cyclical feeding on the growth parameters of dolphin cichlid (Cyrtocara moorii Boulenger, 1902). In the study, 300 dolphin cichlid with an initial average weight of 1.12 ± 0.23 g were placed in 15 aquariums (each of them were 60 L). The five experimental groups, were designed as; the control group (A), feeding until satiation twice a day for 90 days, starvation for 1 week (B), starvation for 2 weeks (C), starvation for 1 day/satiation for 3 days (D), starvation for 1 day/satiation for 1 day (E); and the study was carried out in three repetitions. At the end of the study, groups B, C and D showed partial compensatory growth. The highest weight gains and specific growth rates were observed in control group (A), followed by groups B, C and D (p > 0.05). The lowest weight gain was observed in group E (p < 0.05). The feed conversion ratio did not show any significant difference between the groups (p > 0.05). In terms of feed consumption ratio, Control, C and D groups consumed similar amounts of feed (p > 0.05), while B and E groups consumed less feed (p < 0.05) than the other groups.     

Replacement of fish oil with a DHA‐rich Schizochytrium meal on growth performance,activities of digestive enzyme and fatty acid profile of Pacific white shrimp (Litopenaeus vannamei) larvae

This trial was conducted to evaluate the effects of replacing dietary fish oil with Schizochytrium meal for Pacific white shrimp (Litopenaeus vannamei) larvae (initial body weight 4.21 ± 0.10 mg). Six test microdiets were formulated using Schizochytrium meal to replace 0 g/kg, 250 g/kg, 500 g/kg, 750 g/kg, 1000 g/kg or 1500 g/kg fish oil DHA. No significant differences were observed in survival, growth, final body length and activities of digestive enzyme among shrimp fed different diets (p > .05). No significant differences were observed in C20:5n‐3 (EPA) in muscle samples (p > .05). C18:3n‐3 and C20:4n‐6 in muscle increased as Schizochytrium meal replacement level increased (p < .05). No significant differences were observed in C22:6n‐3 (DHA) and n‐3 fatty acids among shrimp fed diets that algae meal replaced 0 g/kg ‐ 1000 g/kg of fish oil. Shrimp fed diet R150 had higher DHA content than other groups and had higher n‐3 fatty acids than that of shrimp fed diets R50, R75 and R100 (p < .05). C18:2n‐6, PUFA and n‐6 fatty acids in muscle increased, while n‐3/n‐6 ratio decreased with increasing algae meal replacement level from 0 g/kg to 1000 g/kg (p < .05). In conclusion, Schizochytrium meal could replace 1500 g/kg fish oil DHA in the microdiets without negatively affecting shrimp larvae survival, growth and activities of digestive enzyme.     

Effects of juniper berry oil on growth performance and blood parameters in common carp (Cyprinus carpio)

In this study the effects of commercial juniper berry oil (JBO) as a feed additive in diets for common carp (Cyprinus carpio) was investigated in terms of growth performance, feed utilization and blood parameters. The experimental diets were prepared to contain JBO at rate of 0 (JBO‐0), 5 (JBO‐5) and 10 (JBO‐10) ml/kg. Common carps (3.07 ± 0.15 g) were fed with test diets for a period of 60 days in 40‐L aquariums of triplicate groups. The feeding experiment revealed that JBO groups showed significantly better growth performance and feed utilization compared with the control group (JBO‐0) (p < 0.05). No significant effect was observed on hemoglobin amount, haematocrit ratio and consequential erythrocyte indices (p > 0.05). In the JBO groups, serum cholesterol, triglyceride, glutamic–pyruvic transaminase and alkaline phosphatase levels decreased significantly (p < 0.05). In contrast, the total protein level showed a significant increase, while glucose, albumin and lactate dehydrogenase levels did not present any significant variation (p > 0.05). Therefore, dietary addition of 5 ml/kg JBO promoted fish growth and positively influenced the blood parameters in common carps.     

Effects of dietary supplementation of Astragalus membranaceus,Codonopsis pilosula,and Glycyrrhiza uralensis extract mixture on growth performance,haematological parameters and hepatopancreatic performance in juvenile Pacific white shrimp (Litopenaeus vannamei)

Pacific white shrimp is the major farmed shrimp species in the world. It is known to be very sensitive to the environmental and management changes such as intensification, which is one of the primary necessities to increase shrimp production, but represents a stressful condition, and needs to be managed properly to diminish its negative effects in aquaculture. In this study, juvenile Pacific white shrimp were fed diets supplementing with equal quantities of Astragalus membranaceus, Codonopsis pilosula and Glycyrrhiza uralensis extracts in different concentrations (0, 1.0, 2.0 and 3.0 g/kg) for 8 weeks. We subsequently estimated the effects of supplemented diets on growth performance, haematological parameters, and histological changes of hepatopancreas. Results revealed that the supplemented mixture had no benefits on growth and survival rate, but had favourable effects on haemocyte count, number of granulocytes, and hemocyanin concentration in the haemolymph (p > 0.05). We observed that R cells and B cells were increased in hepatopancreatic tubules of shrimps fed on supplemented diets. Besides, a diet containing 2.0 g/kg of extract mixture maintained the decreased levels of alanine aminotransferase (p < 0.05) and aspartate aminotransferase (p > 0.05). Among the used concentrations, 2.0 g/kg seemed to be the most suitable concentration.     

Effects of dietary Bacillus pumilus on growth performance,innate immunity and digestive enzymes of giant freshwater prawns (Macrobrachium rosenbergii)

The present study was conducted to investigate the effects of Bacillus pumilus on the growth performance, innate immunity and digestive enzymes of the giant freshwater prawn, Macrobrachium rosenbergii. Prawns (mean weight: 1.81 ± 0.01 g) were fed different levels of B. pumilus at doses of 0, 1 × 10⁷, 1 × 10⁸ and 1 × 10⁹ CFU/g to produce one control group and three experimental groups (G1, G2 and G3), respectively. After a 60‐day experimental feeding period, the results showed that the final weight, weight gain rate (WGR) and specific growth rate (SGR) were significantly higher in experimental groups than in the control (p < 0.05), whereas these improvements did not differ significantly (p > 0.05) among experimental groups. The improvements in phagocytic activity, respiratory burst (RBs) activity and the activity of catalase (CAT), nitric oxide synthase (NOS) and acid phosphatase (ACP) were significantly higher in G2 and G3 than in the control and G1 (p < 0.05). Further, the activity of CAT, NOS and ACP significantly increased with an increase in the dietary level of B. pumilus (p < 0.05). The activity of phenoloxidase (PO) in G3 was significantly higher than that in the control (p < 0.05). The activity of amylase was significantly higher in the groups G2 and G3 than that in the control group. G3 improved the activity of protease compared with the control (p < 0.05). In conclusion, the results of this study indicated that the potential use of B. pumilusat a dose of 1 × 10⁸ CFU/g feed could improve the growth, immunity and digestive enzymes of M. rosenbergii.     

Effect of food restriction on female reproductive performance in the redclaw crayfish Cherax quadricarinatus (Parastacidae,Decapoda)

Aquaculture activity has grown remarkably in the last years, crustaceans being the most profitable products because of their high prices. Feeding costs represent a large portion of total operating costs in the aquaculture industry. In this context, the objective of the present study was to evaluate the effect of food restriction on the reproductive performance of redclaw crayfish Cherax quadricarinatus. Females at the onset of sexual maturity were stocked with males and were fed daily a formulated diet at 1.5% (control group) and 0.5% (restricted group) of their mean weight. The experimental period lasted 105 days. The percentage of ovigerous females and broods successfully hatched tended to be higher in restricted females compared with control females, but egg volume and weight did not differ between both experimental groups. However, the lipid concentration of rematuring ovaries was lower in restricted females than control females, suggesting that food restriction affects the amount of reserves transferred to the ovaries during vitellogenesis. The nutritional state of females was independent of the amount of food provided, as reflected by similar growth parameters and biochemical composition of the hepatopancreas in control and restricted females. Based on these results, we conclude that it is possible to reduce the feeding rate of C. quadricarinatus females to one‐third of its original value without affecting their reproductive performance and somatic growth, at least for a 105‐day period. This finding provides a useful tool for commercial producers to decrease production costs and improve water quality in culture systems.     

A Comparison between dietary effects of Cuminum cyminum essential oil and Cuminum cyminum essential oil,loaded with iron nanoparticles,on growth performance,immunity and antioxidant indicators of white leg shrimp (Litopenaeus vannamei)

This study evaluates the effect of dietary cumin essential oil's (CEO) and cumin essential oil's loaded iron nanoparticles (CEO‐loaded NPs) on growth performance, haematological parameters and antioxidant status of white leg shrimp. Shrimps with the initial weight of 3.38 ± 0.014 g were allocated into 15 fibreglass tanks (300 L, 60 shrimps per tank) and were fed with five experimental diets containing 0 g/kg CEO (the control diet), 5 g/kg CEO, 10 g/kg CEO, 5 g/kg CEO‐NPs and 10 g/kg CEO‐NPs for 56 days. After 56 days of culture, the shrimps fed with CEO and CEO‐loaded NPs supplemented diets showed a more significant increase in growth performance (p < .05) than in the control diet. Total haemocyte count (THC) value, large‐granule cells (LGC), hyaline cells (HC)%, superoxide dismutase (SOD) and Alkaline phosphatase (AKP) activity were significantly higher (p < .05) in the shrimps fed with CEO and CEO‐loaded NPs supplemented diets than the shrimps fed with the control diet. Glutathione peroxidase (GPX) enzyme activities along with the glucose and cholesterol concentrations significantly decreased in the shrimps fed with the CEO and CEO‐loaded NPs supplemented diets more than those fed with the control diet. The results suggest that the dietary CEO‐loaded NPs can improve the growth performance, the health status and the antioxidant capacity of L. vannamei.     

Improvement of reproductive indices,lysozyme activity,and disease resistance in live‐bearing ornamental fish,Poecilia latipinna using Artemia supplementation with treated yeast cell,Saccharomyces cerevisiae

In the present study, the effects of Artemia supplemented with 2‐β‐mercapto‐ethanol (β‐ME) treated yeast cell, Saccharomyces cerevisiae, on growth and reproductive performance, lysozyme activity, and disease resistance to Aeromonas hydrophila of freshwater ornamental species, Poecilia latipinna, were investigated. Within 60 days, molly fish were fed with three treatments including commercial food (T1), un‐supplemented Artemia (T2), and Artemia supplemented with β‐ME‐treated yeast cell (at concentration of 4 × 10⁷ CFU/L of water) (T3). After the feeding period, the fish were exposed to 100 μl of a suspension (1.1 × 10⁷ cells/ml) of A. hydrophila (BCCM5/LMG3279) and the cumulative mortality rates were recorded for 12 days. No significant difference was found between survival rate and growth performance of P. latipinna except for weight gain that was higher in fish fed through Artemia supplemented with β‐ME‐treated yeast cell compared to control group. Fecundity rate was significantly improved in fish fed using T3 with the maximum amount of 49.5 ± 2.29 per female (p < 0.05). Besides, lysozyme activity was significantly increased in group 3 (p < 0.05). Moreover, lowest fish mortality was significantly observed in this treatment (p < 0.05). In addition, the number of colonies formed by yeast cell in T3 (634 × 10³ CFU/g intestine) showed significant difference with other treatments (p < 0.05). In sum, Artemia enriched with β‐ME‐treated yeast improved reproductive indices, immune responses, and resistance against A. hydrophila of P. latipinna.     

Effects of native medicinal herbs (Alternanthera sessilis,Eclipta alba and Cissus quadrangularis) on growth performance,digestive enzymes and biochemical constituents of the monsoon river prawn Macrobrachium malcolmsonii

The medicinal herbs such as Alternanthera sessilis, Eclipta alba and Cissus quadrangularis were used to assess the growth promoting ability, enhancement of muscle biochemical constituents and profiles of amino acid in the freshwater prawn, Macrobrachium malcolmsonii. The herbal powders were separately incorporated with basal diet, and these diets were fed to M. malcolmsonii postlarvae for a period of 90 days. At the end of experiments, the survival of prawn significantly improved in herbal incorporated feed. In addition, the growth parameters, such as weight gain, specific growth rate and protein efficiency rate were significantly (P < 0.05) higher in herbal diets fed groups when compared with control. The activities of digestive enzyme, biochemical constituents, profile of amino acid, energy utilization parameters, concentrations of vitamin C and E were significantly (P < 0.05) elevated in experimental diets fed groups when compared with control. The profiles of essential amino acids were also found elevated in herbal incorporated diets fed PL. Among the three herbs tested, C. quadrangularis incorporated diet fed group showed better performance followed by the E. alba and A. sessilis. The present study suggests that these herbs can be incorporated in artificial feed formulations for Macrobrachium culture.     

Effects of dietary zinc oxide and selenium nanoparticles on growth performance,immune responses and enzyme activity in rohu,Labeo rohita (Hamilton)

A 120‐day feeding trial was conducted to assess the effects of dietary supplementation of zinc oxide (ZnO) and selenium (Se) nanoparticles (NPs) on growth, immunological and enzymatic profiles in rohu, Labeo rohita. Fishes were fed with basal diet incorporated with laboratory‐synthesized nanoparticles, viz., zinc oxide nanoparticles—10 mg/kg and Se nanoparticles—0.3 mg/kg throughout the experimental period. At an interval of every 30 days, treated and control fishes were randomly collected and subjected to estimation of growth, non‐specific immune parameters and serum enzyme assays. It was observed that there was significant (p < 0.05) increase in growth and non‐specific immune parameters like respiratory burst, lysozyme and myeloperoxidase activities as compared to the control group (only basal diet). Serum enzymatic profiles such as lactate dehydrogenase and alkaline phosphatase activities down‐regulated (p < 0.05) in treated group compared to control group, and superoxide dismutase and acetylcholine esterase activity up‐regulated in treated group. The relative percentage survival (RPS) found to be significantly higher in treated groups (60.00 ± 8.82%) as compared to control group (45.00 ± 6.17%) while challenged with virulent bacterial strain Aeromonas hydrophila. These results indicated that dietary supplementation of zinc oxide (ZnO) and selenium (Se) nanoparticles stimulates immunity and enhances resistance to bacterial infection in L. rohita.     

Effect of replacing dietary fish oil with soybean oil on growth performance,fatty acid composition and haematological parameters of juvenile meagre,Argyrosomus regius

A nutrition trial with meagre, Argyrosomus regius was assessed to determine the effect of dietary replacement of fish oil (FO) by soybean oil (SO) on the growth, feed utilization, body composition, fatty acid composition and basic haematological parameters. Six isonitrogenous (47% crude protein) and isoenergetic (gross energy 22 kJ/g) experimental diets were formulated by replacing 0 (FO), 20 (S20), 40 (S40), 60 (S60), 80 (S80) and 100 (S100) % of the FO with SO. Fish were fed three times daily to near satiation for 14 weeks. The specific growth rate (SGR) of fish fed S100 diet was significantly lower than the other treatments, except SO80 diet. The fish fed SO100 diet displayed significantly higher feed conversion ratio than that of other diets (P < 0.05). It was observed that fish fed the SO100 and SO80 diets displayed haemoglobin (HGB) levels significantly lower (P < 0.05) than fish fed the SO20 diet. Packed cell volume (PCV) of fish fed SO20 diet was significantly higher compared to SO100. The white blood cell (WBC) and red blood cell (RBC) remained unaffected by dietary treatment. The docosahexaenoic acid (22:6n‐3, DHA) and eicosapentaenoic acid (20:5n‐3, EPA) levels of meagre were significantly reduced by the substituting of dietary SO by FO at the end of the feeding period. The level of linoleic acid (18:2n‐6, LA) and linolenic acid (18:3n‐3, LNA) significantly raised in fish fed with SO diets (P < 0.05). The results of this study showed that SO could be replaced FO up to 80% in meagre diet without negative effect on growth performance and basic haematological parameters. Furthermore, the maximum level of FO replacement with SO determined by second order polynomial regression analysis, was 30.1% on the basis of maximum SGR.     

Dietary administration of natural immunostimulants on growth performance,haematological, biochemical parameters and disease resistance of Asian Sea bass Lates calcarifer (Bloch, 1790)

This study was aimed to address the promising evaluation of Cissus quadrangularis plant (stem) and lipopolysaccharide (LPS from bacteria) supplemented diets on innate immune response in Lates calcarifer fingerlings against Aeromonas hydrophila infection. Fingerlings were fed supplemented diets containing four different concentrations of C. quadrangularis (0.5, 1.0, 1.5 and 2.0 g kg^?1 feed), LPS (25, 50, 75 and 100 mg kg^?1 feed) and control (normal formulated diet) for 60 days. The fish fingerlings fed supplemented diet displayed significant differences (P < 0.05) in specific growth rate (SGR) and relative percentage survival compared to the control group fed without C. quadrangularis and LPS‐supplemented diet. Fingerlings were injected intraperitoneally with 100 μL lethal dose of A. hydrophila containing 1 × 10⁶ CFU g^?1. Supplementation of C. quadrangularis and LPS diet significantly increased biochemical profile such as protein, lipid and carbohydrate content, haematological parameters of L. calcarifer fingerlings in different experimental periods when compared with the control group. Dietary doses of C. quadrangularis and LPS‐supplemented diet significantly influenced growth performance and increased survival rate in L. calcarifer fingerlings against A. hydrophila infection.